LOCUS       DASJON010000001        56076 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJON010000001 DASJON010000000
VERSION     DASJON010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.001"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..1072
                     /gene="mvk"
                     /locus_tag="U3989_000001"
     CDS             128..1072
                     /gene="mvk"
                     /locus_tag="U3989_000001"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN1713483.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1065..2042
                     /gene="mvaD"
                     /locus_tag="U3989_000002"
     CDS             1065..2042
                     /gene="mvaD"
                     /locus_tag="U3989_000002"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN1713484.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2055..3140
                     /locus_tag="U3989_000003"
     CDS             2055..3140
                     /locus_tag="U3989_000003"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN1713485.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3137..4201
                     /locus_tag="U3989_000004"
     CDS             3137..4201
                     /locus_tag="U3989_000004"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN1713486.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4450..5253)
                     /locus_tag="U3989_000005"
     CDS             complement(4450..5253)
                     /locus_tag="U3989_000005"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN1713487.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5273..6115)
                     /gene="kduI"
                     /locus_tag="U3989_000006"
     CDS             complement(5273..6115)
                     /gene="kduI"
                     /locus_tag="U3989_000006"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN1713488.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6131..7138)
                     /locus_tag="U3989_000007"
     CDS             complement(6131..7138)
                     /locus_tag="U3989_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN1713489.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7145..7789)
                     /locus_tag="U3989_000008"
     CDS             complement(7145..7789)
                     /locus_tag="U3989_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN1713490.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7889..8647)
                     /locus_tag="U3989_000009"
     CDS             complement(7889..8647)
                     /locus_tag="U3989_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN1713491.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8774..9925)
                     /gene="fucO"
                     /locus_tag="U3989_000010"
     CDS             complement(8774..9925)
                     /gene="fucO"
                     /locus_tag="U3989_000010"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN1713492.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(10007..10318)
                     /gene="rhaM"
                     /locus_tag="U3989_000011"
     CDS             complement(10007..10318)
                     /gene="rhaM"
                     /locus_tag="U3989_000011"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN1713493.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(10320..11165)
                     /gene="rhaD"
                     /locus_tag="U3989_000012"
     CDS             complement(10320..11165)
                     /gene="rhaD"
                     /locus_tag="U3989_000012"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN1713494.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(11179..12435)
                     /gene="rhaA"
                     /locus_tag="U3989_000013"
     CDS             complement(11179..12435)
                     /gene="rhaA"
                     /locus_tag="U3989_000013"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN1713495.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(12432..13892)
                     /gene="rhaB"
                     /locus_tag="U3989_000014"
     CDS             complement(12432..13892)
                     /gene="rhaB"
                     /locus_tag="U3989_000014"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN1713496.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            14045..14914
                     /locus_tag="U3989_000015"
     CDS             14045..14914
                     /locus_tag="U3989_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN1713497.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(15040..15939)
                     /locus_tag="U3989_000016"
     CDS             complement(15040..15939)
                     /locus_tag="U3989_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1713498.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16104..16928)
                     /locus_tag="U3989_000017"
     CDS             complement(16104..16928)
                     /locus_tag="U3989_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1713499.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(16939..18234)
                     /locus_tag="U3989_000018"
     CDS             complement(16939..18234)
                     /locus_tag="U3989_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN1713500.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(18250..18729)
                     /locus_tag="U3989_000019"
     CDS             complement(18250..18729)
                     /locus_tag="U3989_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN1713501.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(18735..19763)
                     /locus_tag="U3989_000020"
     CDS             complement(18735..19763)
                     /locus_tag="U3989_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN1713502.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(19780..20886)
                     /locus_tag="U3989_000021"
     CDS             complement(19780..20886)
                     /locus_tag="U3989_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1713503.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(21115..23712)
                     /gene="adhE"
                     /locus_tag="U3989_000022"
                     /gene_synonym="adhC"
     CDS             complement(21115..23712)
                     /gene="adhE"
                     /locus_tag="U3989_000022"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN1713504.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(24017..24397)
                     /gene="yajC"
                     /locus_tag="U3989_000023"
     CDS             complement(24017..24397)
                     /gene="yajC"
                     /locus_tag="U3989_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN1713505.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(24494..25639)
                     /gene="tgt"
                     /locus_tag="U3989_000024"
     CDS             complement(24494..25639)
                     /gene="tgt"
                     /locus_tag="U3989_000024"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN1713506.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            25857..26555
                     /locus_tag="U3989_000025"
     CDS             25857..26555
                     /locus_tag="U3989_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1713507.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(26723..29362)
                     /locus_tag="U3989_000026"
     CDS             complement(26723..29362)
                     /locus_tag="U3989_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1713508.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(29981..31012)
                     /gene="queA"
                     /locus_tag="U3989_000027"
     CDS             complement(29981..31012)
                     /gene="queA"
                     /locus_tag="U3989_000027"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN1713509.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            31205..32379
                     /locus_tag="U3989_000028"
                     /pseudo
     CDS             31205..32379
                     /locus_tag="U3989_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            32383..33024
                     /locus_tag="U3989_000029"
     CDS             32383..33024
                     /locus_tag="U3989_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713510.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            33021..33938
                     /locus_tag="U3989_000030"
     CDS             33021..33938
                     /locus_tag="U3989_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1713511.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            33935..34606
                     /locus_tag="U3989_000031"
     CDS             33935..34606
                     /locus_tag="U3989_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713512.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(35147..35635)
                     /locus_tag="U3989_000032"
     CDS             complement(35147..35635)
                     /locus_tag="U3989_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN1713513.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(35836..36102)
                     /locus_tag="U3989_000033"
     CDS             complement(35836..36102)
                     /locus_tag="U3989_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1713514.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(36102..36365)
                     /locus_tag="U3989_000034"
     CDS             complement(36102..36365)
                     /locus_tag="U3989_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1713515.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            36841..37026
                     /locus_tag="U3989_000035"
     CDS             36841..37026
                     /locus_tag="U3989_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1713516.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(37168..38991)
                     /locus_tag="U3989_000036"
     CDS             complement(37168..38991)
                     /locus_tag="U3989_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN1713517.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(39589..42294)
                     /locus_tag="U3989_000037"
     CDS             complement(39589..42294)
                     /locus_tag="U3989_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN1713518.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(42600..43547)
                     /locus_tag="U3989_000038"
     CDS             complement(42600..43547)
                     /locus_tag="U3989_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN1713519.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            43737..44006
                     /gene="rpsN"
                     /locus_tag="U3989_000039"
     CDS             43737..44006
                     /gene="rpsN"
                     /locus_tag="U3989_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1713520.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(44182..44544)
                     /locus_tag="U3989_000040"
     CDS             complement(44182..44544)
                     /locus_tag="U3989_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1713521.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(44555..45676)
                     /locus_tag="U3989_000041"
     CDS             complement(44555..45676)
                     /locus_tag="U3989_000041"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN1713522.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(45692..46042)
                     /locus_tag="U3989_000042"
     CDS             complement(45692..46042)
                     /locus_tag="U3989_000042"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN1713523.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(46172..47683)
                     /locus_tag="U3989_000043"
     CDS             complement(46172..47683)
                     /locus_tag="U3989_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1713524.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(48005..49357)
                     /locus_tag="U3989_000044"
     CDS             complement(48005..49357)
                     /locus_tag="U3989_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN1713525.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(49510..50586)
                     /locus_tag="U3989_000045"
     CDS             complement(49510..50586)
                     /locus_tag="U3989_000045"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN1713526.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            50782..52527
                     /locus_tag="U3989_000046"
     CDS             50782..52527
                     /locus_tag="U3989_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN1713527.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(52807..53244)
                     /locus_tag="U3989_000047"
     CDS             complement(52807..53244)
                     /locus_tag="U3989_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN1713528.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(53228..54601)
                     /locus_tag="U3989_000048"
     CDS             complement(53228..54601)
                     /locus_tag="U3989_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713529.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(54923..55411)
                     /locus_tag="U3989_000049"
     CDS             complement(54923..55411)
                     /locus_tag="U3989_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1713530.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(55433..56035)
                     /locus_tag="U3989_000050"
     CDS             complement(55433..56035)
                     /locus_tag="U3989_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1713531.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        15942 a        12171 c        10271 g        17692 t
ORIGIN      
        1 agtaaaataa aggaggaatg ttttttgaga cccttagtgg acaaaaaaat tcgtgaagca
       61 gcgcaactcg ttttgggcgc tttatttttt gcttaaaaat cacttttacg tgagaaggag
      121 ttagattatg gcaaactatg gccaaggaga atcaagcgga aagatcatat tgatgggcga
      181 gcacgcggtt gtttatggag aaccagcgat tgcctttcct ttctatgcaa caaaagtcac
      241 cgcattcctt gaagagctgg atgcaatgga cgatcaactg gtttcttcct actattcagg
      301 aaatttagcc gaagctcctc atgcattaaa aaatatcaaa aaattattca ttcacttaaa
      361 aaaacagcat gacatccaaa aaaacttgca actgaccatt gaaagcacga ttcctgctga
      421 acgtggaatg ggatcaagcg ctgcagtcgc cacagcagtc actcgtgctt tttatgatta
      481 cttagcattt cctttgtctc gtgaaatact attagaaaat gtccagcttt cggaaaaaat
      541 cgcccatggt aatcctagtg gaatcgatgc agccgctact agcagcttgc agccgattta
      601 ttttacaaaa gggcatcctt tcgactactt ttctttgaac atcgatgctt ttttgattgt
      661 cgctgataca ggaatcaaag gacaaacaag agaagccgtc aaagatgtcg ctcacctctt
      721 tgaaacccag cctcatgaaa ctggacaaat gatccaaaaa ttaggatact tgacgaagca
      781 agcaaaacaa gcaatcattg aaaattcacc agaaacgttg gcacagacaa tggatgaatc
      841 acaatcactt ctggaaaagc tgacaatcag caatgatttt cttaatctat tgatccaaac
      901 agcaaaagat accggggcat tgggtgccaa attaactggc ggtggacgcg gtggctgtat
      961 gattgcatta gcacaaacaa aaacaaaagc ccaagaaatc agtcaagcac ttgaagatgc
     1021 gggtgctgct gaaacttgga tacaaggatt aggagtacat acctatgttt aaaggcaaag
     1081 cacgcgcata taccaacatt gctctaatca aatattgggg taagaaaaat gaagaactca
     1141 tccttccaat gaacaacagc ctttcgttaa cattggatgc attttataca gagacagaag
     1201 tcatcttttc tgatagctat atggtagatg aattttatct agatggcacc ttgcaagacg
     1261 aaaaagcaac aaaaaaagtc agtcagtttc ttgacctttt tcgtaaagaa gccggcctct
     1321 cactaaaagc ttcagtaatc agtcagaatt tcgttcctac cgcagctgga ttagcctcct
     1381 ctgccagcgg gctagctgct ttagcaggcg cttgcaatac tgctcttaag cttggattag
     1441 atgatctctc tctttcaaga tttgctcgac gcgggtctgg ttcagcttgc cgaagtattt
     1501 tcggtggttt cgtcgaatgg gaaaaaggcc atgacgactt aagttcttac gctaagccag
     1561 tcccttccga ttctttcgaa gacgatttag caatggtttt cgttttgatc aacgaccaga
     1621 aaaaagaagt gtccagcaga aatgggatgc gtcggacagt cgaaacatcc agtttttatc
     1681 aaggctggtt agattccgtt gaaggggatc tatatcaatt gaaacaagca atcaaaacaa
     1741 aagatttcca acttctcgga gaaacgatgg aaagaaacgg actaaaaatg catggaacga
     1801 ctttagctgc ccagcctcca ttcacttact ggtctcctga cagcttaaaa gcgatggatg
     1861 cggtgcgaca attgagaaag caaggcattc catgctactt tacgatggat gctggcccta
     1921 atgtcaaagt tttagtggaa aacagccatt tatctgaggt acaagaaaca tttaccaaat
     1981 tatttagcaa agaacaagta attactgcac atgctggtcc aggaattgcg attattgaat
     2041 aaagggagat ttttatgatt gaagtatctg caccaggcaa actctatatt gccggagaat
     2101 atgcagttgt tgaaacaggc catccagcag ttatcgctgc agtcgatcaa ttcgtgacag
     2161 tcactgtaga atctgcacga aaagtcggaa gtatccaatc tgcacaatat agtgggatgc
     2221 ctgtacgttg gacaagacgc aacggagaat tagttttaga tattcgggaa aatccttttc
     2281 attatatcct tgctgctatt cgcttgactg aaaagtatgc acaagaaaaa aacattcttt
     2341 tatcctttta tgatctgaaa gtaacaagtg agttagacag ttcaaatggc cggaaatatg
     2401 gattgggttc tagcggtgcc gtgaccgttg caacggtcaa agccttgaat gttttttacg
     2461 cgttgaattt atctcagttg gagattttca agattgcagc actagccaat ttagcagttc
     2521 aagataatgg ttcctgcggc gacatcgctg ctagctgtta tggtggctgg atcgctttct
     2581 caaccttcga ccatccttgg ctccaagaac aagaaactca gcattctatc agtgagttac
     2641 ttgccctgga ttggccaggt ctatccattg agccattgat tgctcctgaa gatttacgtt
     2701 tattgattgg ttggacgggt agccctgcct ctacttctga tttggtcgat caagttcacc
     2761 gttcgagaga agataaaatg gtggcttatc agcttttctt aaaaaatagt acagaatgtg
     2821 tcaacgaaat gatcaaaggg tttaaagaaa ataatgtaac gttgatccaa cagatgattc
     2881 gaaaaaaccg acaattactg catgatttat ctgcaatcac tggggtcgtc atcgaaacgc
     2941 ctgctttgaa caaattgtgt aatttagctg aacagtatga aggagccgca aaatcttctg
     3001 gtgcaggtgg gggcgattgc ggaatcgtaa ttgttgacca gaaatctggc attcttcctt
     3061 taatgagtgc atgggaaaaa gcagaaatca ctccactgcc gttacatgtc tatagcgatc
     3121 aaagaaagga aaaccgatga atcgaaaaga cgaacatgtt tcactagcta aagcctttca
     3181 tgataaacaa aaaaacgaat ttgacttcgt acggatcatc cataaccctt tgccgcaaat
     3241 agcagtttct gatgtcgatc taagtacaca ggcagttggg tttacactaa gcagcccatt
     3301 ttatatcaat gcgatgactg gcggaagcga aaaaacaaaa aaaatcaacc aagatcttgc
     3361 aatcgtcgca agagaagcag atttgatgat tgccactggt tcagtcagcg ctgccttgaa
     3421 agtcccttct ttagccgata cttatacgat tatgcgacaa gaatatcctc acgggaaaat
     3481 cattgccaac atcggtgcag ggacttctgt cgaaagagcc aaagaagcta tccgattgtt
     3541 tcacgcagat gccttacaga ttcatttgaa tgcacctcaa gagttggtta tgcctgaggg
     3601 agaccgtgat tttaccaatt ggaaagtact tattcaagaa actcaaacag ccattgacgt
     3661 ccccctcatc gtaaaagaag tcggttttgg catgacaaga gaaacgctca atgatctagc
     3721 tgctttagga gttcatacag ttgatatcag cggtcgaagc ggaacaagct tcacccagat
     3781 cgaaaacgcc cgtcgctcca agcgggaact gagttattta gctgactggg ggcaatcgac
     3841 agtctcttca ttgcttgaag caaatgaagc agacacttct atggaaatcc tagcctccgg
     3901 aggtatccgc aatgcctatg acatatttaa agccctttgt ctcggcgcaa atgccgtggg
     3961 tacttcagga accgtactga cgcatctgat gaatcacggc gtagaagaaa ctattatact
     4021 gatgaagcaa tggcaagaag agcttcgtct gctttatact atggtcggcg caacgaatac
     4081 cgcggcccta caccaacaat ctctgatctt ttccggacca gtgaaggatt ggtgcgaagc
     4141 aagagggatc gacttagtga agtatggaag acgcacagaa aagagcgtgg gacaaaagta
     4201 aagaacactt ttgtctcacg ctctaaacac gtacaaacgg cggaagtaaa agcaactcct
     4261 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat tccttcttat
     4321 ttcttggagt taaatgcttc tgtcccagcc tcttttttcg aataaatgat gtttaatcag
     4381 ctgtccttcg gtattcgttc aatccgctga tttcacaacc tctccactaa acagtgtact
     4441 aaagctcttt taaggttgtt ttccgatata ggcaagtatg cctccgtcca catacaagac
     4501 atggccgtta acgaaatcag atgcgctaga agccaagaag acagatggtc cagccaaatc
     4561 gattggctcg ccccagcgtg ctgctggcgt acgtccaacg ataaattgat cgaatggatg
     4621 acgttcaccg ttttcttgtg tttcgcgtaa tggagcagtt tgcggtgtag caatataacc
     4681 tggtccaatt ccattacatt ggatattgta ttgaccgtat tcagaagcga tgtttttcgt
     4741 cagcattttt aatccgcctt ttgccgcagc ataagcactg accgtttcgc gtcctaattc
     4801 actcatcatg gagcagatat tgatgatttt accgccgcct ttttcgatca tatccggaat
     4861 aactgctttt gaaacgatga atggggcatt taaatccacg tcgattactt ggcggaagtc
     4921 tgccgcatcc atttcgatca tcggtgtccg tttgatgatc cctgcattgt tgactagaat
     4981 atcaactgaa cctacatctt ctttgatctg ttgaaccatc gcttgaaccg cttcttcatc
     5041 ggtcacatcg cagacataac cttttgcatc gatgcctgct tctttataat gcgctatacc
     5101 ttcatcaact gattcttgat tcaagttatt aaatacgatt tttgctcctg ctgtagctaa
     5161 agagcgtgca atctcaaatc caataccata gacagcaccg gtgactaatg ctacttttcc
     5221 atctaaacga aacatatcca tactaaattc catcattttc tctcccttat gcttatttta
     5281 attgatccat ttgcaccata tccatgtctg tataggtgat attttctccg cacattgccc
     5341 agatgaatga gtaattgctt gtacctacac ctgaatggat cgaccagctt ggcgaaataa
     5401 tggcttgttc attgtctaca actaaatgtt ttgtttcatc tggtttgccc atcatgtgga
     5461 acacacgtgt atcttcattg gcataatcaa agtagacata agcttccata cgacgttcat
     5521 gtgtatgaca aggcatcgta ttccaagcgc ttcctggttc taaaatcgta taacccattt
     5581 gtaattgaca gctttcgcat acatttggat ggatgtattg gtagatttta cgttcattca
     5641 gtgtcaggcc ttcaccagtc tccattggtt ttacttggtc gatactgatt tttacatttg
     5701 gatatttatg atgagctgga acagaactaa tatagaattt agctgggttt tcagcatctt
     5761 gagaagaaaa tacaacatgt cgtgtttctt ttccgatata gtatccgtct tgttttttca
     5821 tttcttcttt ttgcccatcg atctcgatga atcctggtcc accgatattg atcacaccta
     5881 gttcacgacg ctctaagaag taatcaacac ctagttcttt gtctaatgtg atttctaatt
     5941 ctttttcagt aggtgtcact ccaccaaaga tcattcgatc gttgtgcgta taagtcaaac
     6001 tgattttccc tggttcaaat accttttcta ctaaaaattc ttttctcaat tgttccgttg
     6061 aataatgacg gatatcttct ggactgtgtg tataacgtgt ttccatttct tgcattttct
     6121 tctactcctt ctatcgaatg atttttccag agccgcatgc taaaaattgt gcgacttctt
     6181 cttgactaaa ttggttgcag tccccatgga tcgtatgctt caaggcagaa gctgccgtag
     6241 caaaatcaat gatttcttgc ggagacatat tggataaaat cccatggagc acgccacctg
     6301 caaaagcatc tccgccacct actcgatcca cgattggggt gattctatgg acttgggatt
     6361 ccacatattc atggttcatc cacaatgtcc ccttcaattc attttcactt gctgaatgaa
     6421 cggtacgctt ggtcgaataa aacacctgaa tattcgggaa cttttcctgc attttttgat
     6481 aataataaat tagaggttgt tcttctgctt ctggcgcttt ctcgatacct aacaaataaa
     6541 gagcatccat ttttccagct gagcagatat ctactaaagg taaaagctga tggattactt
     6601 cgctggcttc ttgctgtgtc cataattttc cacgatagtt gatatcgaag ctgatcaggc
     6661 agccagcttt ttttgcagct tctatcacct gtttcgtcat tttttgccat tttttagaca
     6721 aagctggggt gattcctgaa acatgaaata tatctattcc ttggaataac tcctctagcg
     6781 accattccaa atgatccatt tcagcaaaac ttgatccggc acgatcatag atcactgagg
     6841 cggcgcgttc gccactacca gtttccatat aataagtacc tagtcttgat ccaccagata
     6901 atacctgttt cgtatctact ccataacatt gcaagtgctt tttgactgct tttcccaatg
     6961 cgttatcagg tacttttgta gcaaaaatcg cttcatgtcc gtaattagct aaagaaattg
     7021 ctacgtttgc ttcccctccg ccataatggg cagcgaatcg ttcacttgaa tcgatacggg
     7081 ttccctgctc tgtggacaat cgcaacatga tttctccgag tgtcactact tttcccataa
     7141 gcagctacct cttgatttct ttcatttttg ctgcatactg ttgcgcgact tccgttactt
     7201 tgtcaaaatc tcctgtagct gcgggtgcta aaaggttccc acctactccg actgttacga
     7261 caccagcatc gaaccactct ttcatattgt ccagactcac accgccagtt ggcatgatat
     7321 tgacttgagg catcggcgcc ttgaaggcag aaatgatact tggtccataa gcgcttccag
     7381 gaaatagttt gacgatatct accccgcttt tcagcgctgt tttgatctcg gtaatagtca
     7441 tacatcctgg taagtaagga atttgataca gattgcacat ctctgccgtt tcttgatcaa
     7501 aggaagggct aacaatatac tctgcgccag ctaagatagc caagcgagct gtgacagcgt
     7561 ctaatactgt gcctgcgcca atcactattt ctggttggtc tttgtagaat gacaaaagtt
     7621 ctttgattac ttgatctgct tgagggactg taaaagtcaa ctcaatacca gttagaccac
     7681 ctttgatgat tgcgtggcaa gcattcaaag cttcttcttt gctttttccg cgtaccaccg
     7741 caatcactcc ggcgtttttc aatcgagata aaatatcaat ttttttcatc ttctccacct
     7801 atttcctatt tagaaatttt attttataac ttggaaatct atacccatat tatacggcag
     7861 aaaagaaaaa ttgcaaacgc ttttttcatt atttttgaat tctttgctcg atttcgcttt
     7921 ttgtttgctt gagggcactg attatttgtt cttttgtctc atctgttaaa cgatatttcg
     7981 gcatacttac actaaatgca ccaatgattt gttcgttttt cacgattgag gctcctatac
     8041 aaaaaatatc acgttcgatt tcttcatcgt caaatgcgat gtgggtttct tttaccgttt
     8101 tgatttcctt tttcaaccgt aatgcattgg tgatcgtata ttctgtgtaa gggatcaacg
     8161 gatgcttttc aagataatca gtgtactgct cttcactgaa ttctgctaat acggctttgc
     8221 ccatcgcaga attgtataat ggtcgggtga ttccgatttt tgaagacatg cggatcgttt
     8281 gatgttttgg atctagtttg ttcacgtaca agatctcatt gttgtccaat acccctaagt
     8341 ggatagtctc atcgatcttt tgctgcagct gttctaaaaa cggtaaagtc aactctacca
     8401 aatcgatttg ttcaatgttt ttattcgcgt agcgaataag ctttgcacct aaacgatagt
     8461 ttttctctga atttttattt acgtagccga tcatcaacaa agtatctagt attttcaaga
     8521 ctgtagaaga ggtcattccc acaccctttg cgatcgtctg caaagagaca tcttggtttt
     8581 ccgctaaata gtctagtatt ttagaggctt tgatcaatac agttccataa ggctttttat
     8641 tttccatata atattctcct atttctttat aagaaaaatt atagcggaaa taccatcaat
     8701 ttaaaagaga tatccagata taaactacca tttctcttta caggcaattt aatgttgaag
     8761 atccgcttcg tttttacatc aaactttgat ataaagcaat gatttcttct actgtcgttt
     8821 ctcttggatt tcctggtgtg catacatctc gcaaagcatc ttctgcaatt gctggaatat
     8881 ctgcttcttt tacacctaat tcagaaatgg tttctgggat tccaaccgct ttgctcaacc
     8941 gatcgatctc accacaagcc gcttctctta catcttctaa agacatttca gctgctcctt
     9001 taatccctag aacaagagcg atctcacggt acttttctcc agtatattct ttgttatatt
     9061 tcatgacagt tggcaaaagt gctgcacacg ctacgccatg aggaatgttg taccatgcac
     9121 ttaacgggtg agccatcccg tgtaccagtc ctaaaccgac atttgagaag cccattcccg
     9181 caatatactg tcctaatgcc attttttctc gcccattttg atcgccatct acagaagctt
     9241 ccaaagaatg gccgatgatt tcgattgctt ttaaatgcag catatcagtc atttcccaag
     9301 cgccttttgt gataaaccct tcaatagcat gcgtcatagc atccatacct gttgatgcag
     9361 ccaatttttt cggcattccc atcatcatat cgctgtcgat gaacgcgacg attgggatat
     9421 catgaggatc aacacaaaca aattttcggt gatttacttc atctgtgatg acgtagttga
     9481 tcgttacttc tgctgctgtt ccagaagtcg ttggtatagc taatataggt aaacatggat
     9541 ttttcgtatc agcaacacct tctaaactaa tgacgtctga aaactctgga ttcgtcaaaa
     9601 taatcccaat ggcttttgcc gtatcgattg gcgaacctcc accgattgcg atcaaacagt
     9661 ctgcctctgc ctctttaaca aaagccactc catcttttac attttggatc gttggattag
     9721 gtacgatccc gtcgtataaa gcataagcga ttgctgcttt atctagaagt tctgttactt
     9781 ttgttgctat accgtgctcg atcaatcctt tgtcagtaat gacagctgct ttgccgaatc
     9841 ctcgctgctg aaattcaggg acgatcgact caatcgctcc ttttccaaaa tacgatgttt
     9901 catttaaaat cattcgatta ctcattttct attcctccta atttgtatta tttttcacaa
     9961 taaaaacgaa agaaccccaa gcaaccggtt gcccagttgc ttgttgctac agttcaaaga
    10021 ctggcatcaa ttcccaactg actggagagg catctggatt tgtctccatg atatcttcca
    10081 tgtacttcca ccatttttga ttgatagatg tatttgccat tttttcccat aacttttcat
    10141 cttcgatcac taagtaacca aataacaaac ttgtactcgg atcaagatag atcgaatagt
    10201 cgatgacccc gtgttcacga agtgctgtac gcatttctgg ccaaagttca tcatgtcttt
    10261 ttttgtattc ttcttgtttt tctgggtaaa ctttcatgca aaaagctttt ttcaccatgt
    10321 cagacacctg ctttcgtatg aacttcctcc aaataacctg cttttggagt gacaccaaac
    10381 gcatctgcta aatgccacag gttttcgtcc gtgattgttt gcaagattgg tccttgcgct
    10441 ttgacgtacg tatagacttc tgctgccttt tctgctgtct cgatcaaacc aaagacttca
    10501 tccatgtctt ttcctgaccc gtagattcca tgttgtggcc ataaaaccaa tcgagtttct
    10561 ttcatttttt cagctgttgc ttccccaatc tcatttgttc ccggaaccat ccacggaatg
    10621 atggaaattc cttctggaaa aacaactaag cattccgtac acatttgcca tagcgtacga
    10681 ctaaaggaac gttcgtctaa ttcatgcgta aaactcatcg ctaatagatg actagcatgg
    10741 caatgcataa cgatccgatt atctgggtct acttctaaac gggcgatatg actcatgaaa
    10801 tgacttggca actcgcttgt aggaaggcat tcgttttcca gcccccacaa aagttccagt
    10861 gttttgccat cttcagcaac tcgaacgatt ccaagattct cggcaggttc gctcgctaca
    10921 tttttaaaat atcttcctga accagttaca ataaaatagc atcctgctaa acggctggca
    10981 tcaaaaatca tcgagatgcg gcggatcact ttttcaggat ccaagaacgg tgtgatctct
    11041 tcttctttta gtaaataaga aatattcccg ccatttcttt catcccatcc caagcgataa
    11101 agattcgacg tcgtttcgat catttctttc acataaggcg cttgtaaaat atctattttt
    11161 ttcaatatag ccacactcct attttcttaa ttccaacacg tctttttgat attcctggac
    11221 ttcttccagc caatccaaac caactggaac ttgatttttg taacagaaat aattccatac
    11281 atcggcatac gggaaatctt tcaattcttc cgtataagcc attcttgaag taaagtcaaa
    11341 agccagctcc atttctttca atcgctcaac tggttccagc atagctttca gcagtgcttt
    11401 ttgtgtattt ctcgtaccga tgacccaagc agcgatacgg ttgatcgttg catcaaagaa
    11461 atccagaccg atatttgttt tttccaacaa ttcgttacgg actaattctt tagcgatttc
    11521 ctgcaattca tcatccatca aaaccacatg gtctgaatcc caacgcactg gacgggaaac
    11581 atgcaacatg attcctttgc tgaataaagc taatgaagat aatttattgg aaatcacttc
    11641 tgttggatga aaatgtccag catctagaca aacaagtttg tcacgagtca acccgtaacc
    11701 catataaaat tcatgggaac cgactgtata agcttcagcg cctaaaccaa atagtttgct
    11761 ttctactgca tcttgtacat accgttcatc taactcttct gtaaaaattt catcgagtga
    11821 ttccattaaa cgttcccgtg gggtaaaacg atcaatcgga ttatctttca tcccatcagg
    11881 tacccaaaag ttattgatac ttgtttgccc agtttctttc ccaaagtact ccgcgatttt
    11941 gcggcttctt ttcccgtgtt cgatccagta ttcacgaact tctttatctg gatgagccaa
    12001 tgtaaagccg tctttcatca ttggatgcga aaagaaagtc ggattgaagt caagaccgat
    12061 ttcttctttt tttgcccact ctacccattt ttcaaaatgt ttcggctcaa tttcatttag
    12121 atcgacagat tcttctgtat ccaaatagat agcatgaagg ttcaatttgt gtttgcccgg
    12181 tatcaacgaa taagccttct ccagatcttg tctaagctct tcaggtgttc gtgcggctcc
    12241 tggaaaattc cctgtcgcca tgattccacc ggtcaattcg ccatccggtg tcaagaaacc
    12301 tttgacatca tctccttgcc agcaatgcat agaaattttg atgtcctgta tcttttctag
    12361 tacagcatct gtatctacac cgatagttgc atatttttct tttgcttgtt cgtaatttct
    12421 ttcaatcgtg ttcatacgat tactcctttg attttattca cgtaccattc catcgtgttt
    12481 tgcttatttt catctgggta aaaagtttca catggaaacg actgtttgat tacatttctt
    12541 gcttgttcta tattcgaaaa ctgacctgta ctgagcattt gcatggcaac attcccgatt
    12601 gctgtggctt cactaggtcc tgcttctaca atgattccgg cataatctgc tgtcaattga
    12661 ttcagtagac gattattcga tccgccccct acgatgtgca gcttttccaa tttcgttcca
    12721 gttaagtgtt ctactttgat cagctcttgt gcgtaacaaa gagcaagatt atcatacaca
    12781 caacgagcga tagctcccga tgagacaggg actggctgct ctgtttcaaa acagtattgc
    12841 tgaatctctt ctatcatatt tgggggattc aaaaatcgag ggtcattgat ttccatcact
    12901 tgctgcagcg gtttttcttt tgcagcaagc tctgccaatt ccgcataact gtactcgtac
    12961 tcttgcattc gggcaacttc ctggatcaac cacatcccca tgatgttttt caaaaaacgc
    13021 accgtgttat gggcacccca ttcattcgta taattttcag aaaaagcatt gcgagaaaca
    13081 ttggcgatct ttgtttcaat ccctaaaagc gaccatgtcc cactgctgat ataaccccaa
    13141 cgttctcctt gacctggcgt tcctaataca gctgacgccg tatcatgact tgctactgtg
    13201 ataaaggtca catctggcaa atcatatttt gaaaattgtt ctttttgcag atctcctagt
    13261 atcgttcccg gctctgtcaa tggtggaaat aattccttcg agacaccgat tttttcaaga
    13321 agttctattt cccattcttt tgtccctgca ttcagcagct gcatagtaga tgcattagtt
    13381 ttctccgtca ccatgttacc agtaaagaca taccctaaat aatcaggtat cagtaacaat
    13441 ttatgtgctt ttgccaaatt ttctttttct tcagcaaaca attggaacaa ggtattgaaa
    13501 ggctggatct gaatccctgt ctgctcatac aacttatcta gagaatagat ttcagaaaac
    13561 tgttctactg ctccattggt acgcttgtcc cgataagcaa tcggatcatt tatccgatcc
    13621 ccttgttcat caatcagaca gtaatcgact gcccatgtat cgatccccgc aaaaaggcga
    13681 tcaatccctg tctgctttaa tttttcaaat ccaatcaaaa tctctttgat aagataatcg
    13741 atcttccagc gatcgtgacc gtctttgttc cgaaagccgt ttttgaaccg gtgtacttct
    13801 tccaagatca aagaaccttt tgcatccctc gtacttttca ttagtcgtcc gcttgatgca
    13861 ccgatatcga tggcaacaaa tgttggattc atttctccac ctcttttact ttattgttga
    13921 ttagcacttt attaataaag cgttatcatt tattgacaat gctatcataa tacagaatct
    13981 tatattttgc ttcacactat tttggtatat aattgcatta tcttgacaag ttgggagtgt
    14041 ttacatggat tactgggaaa aagccaacca ctcatggtcg gaagactcaa aaagagtgat
    14101 cttgacgcct acacaaagaa gcaggaattt atttttttat atccaagaaa tcggttactt
    14161 caaagcacca atcccttact atactgaacg agcacattta ccttcttact tgataaagtt
    14221 tactttgtct ggcaaaggtt cgcttacata taatgataat cagcaacagc taaaagctgg
    14281 agacttattt tttattgact gccaagaata tcagcgttat caaacgatca gcgaagaacc
    14341 atgggaaatg gattggatcc atttgaatgg tcagaacgtc caatcatttt atcaggagtt
    14401 cacaaaagac ggccataaca ccttccattc aacaggagat ccatcagaaa acaagatcca
    14461 ccatttgatc acagaattga tccacatgca acaaggcacg aatgcccgta ccgatttcca
    14521 gtcctctgta gctatccacg agttgctgaa tgaactgatt ttgcaaaagt atcagctaga
    14581 tttcgaagaa gaggatattc ccgcctacgt ttacaaactg aagcaatact tagacgaaca
    14641 tttcaaagaa acgattacac ttgatcaatt agaacaacaa ttcttattga ataaatatca
    14701 gctgagcaaa gatttttcca aatttatcgg ttctccacca atcgattatt tgatcagcaa
    14761 aaaactttct tacgctaaag accttcttcg gtatacagaa tttacggttc gaatgatttc
    14821 cgaagaaata gggattgaga attttgctta ctttagccgc ctgttcaaag caaaaacagg
    14881 actatcgccg agagactttc gtaaattcag ctgattcata taatatgtac gtcatttttt
    14941 caaaatattt tgaaatatat agataaaaat aaaataaccg cagtttctgc ttctatgtaa
    15001 acaacagcag ctactgcggt tattttttag tgatcattct cattgttcat cttcaaaatg
    15061 ttcataatga accaactgtt tctgtgtatc acgtttcaaa aattctttag gcgttatgcc
    15121 gaactctctc ttgaatgctt tcagaaaatg cggataagaa gaaaaaccac ttttactata
    15181 tacagcacta gagctgcggt attctcgcaa caagtgcttg gcatagatca gctttttctt
    15241 cattacatac tgatgaaacg taaatccagt gtacttttta aattctcgtg tgatatggta
    15301 tttgctgaca aaaaactgct tttccatttc ctctaaagaa agcggatgag ataaattttt
    15361 tgagatatat tgtaacattt gttccacacg ctcattagga actacagcat tctgttcttg
    15421 cgcactctct tctttttgta caacttgatt gagaaaaatc aagtattcta ccagagcttg
    15481 ttcatatttt atgtcttcac cgtaaatttt tttatcagga ggatggtcga taaatgataa
    15541 tttttgcttc aatacttcct gttctatttg caatacacga ctcctcttct ctcccattgg
    15601 ttgaaaacaa gccgatagat tagaccatgg cgtggaacga ctttctagaa aagaaggggt
    15661 gacaaagata tagacacgct cgaatacatc tgaagattgg cggacgatac gatgcaaatc
    15721 attggaatgg atgagcagca cacttcctgc tttgatcgta aattgtcgtc cgttcaaata
    15781 aaaagtcgct tctcccttta atgtagcatg gatctcatag aaatcatgat gatgatagat
    15841 cgttttattt tggctgacga catctttgac atgatagatt tcatactgat cagaaaccat
    15901 cgtattgact tgttcaccgc tgattttctt ttggagcatg tgccgcacct ccttttgttc
    15961 acttatgcac aattttatta taccacgttt tttgtaatca aaaagagctg cttttatata
    16021 ctcttataaa aaagaagaga cgtggcatct tctagccccg ccccctccat tctaaatatt
    16081 agttaaccaa cgtcttcttt acattattca agaagacttc ttccataacg aatcccatca
    16141 tcttttgttt cttcaagaac cacatacagg tacggacgat tcttcatgat gatttctttg
    16201 atttttgtgt actcgatcac accgtctccc aaatttaccg gcacgatttt ttcctgttcg
    16261 acacgaaaat ctttcaaatg aaccgcacat atcttttcac caaaacgttc aaaagcttct
    16321 tctaccaatt ggacttgttc ttgataatca ctacttgtga tcaaattggt cggatctaaa
    16381 atgattccta gatagtccga atctactgct tggatcagct gctctaccct tgctaaagaa
    16441 tacaccggat gattcaaacc tggttctatc cccaccatca tttggtattt ttctccagct
    16501 tttaccaagc gacggataac ggacacagct tcagcaaatg cttcatctgt gaaattttct
    16561 tccgtatatt ggatttccgg caaaacacat ccagtttccg aagcaaccat cgacgcacca
    16621 aaggaagaag catagcgaag atactgctcg aactttgtca gcaactcttg tcgtatcgtt
    16681 aaatctggat gaatcatatt gatatagcaa cttaaaattc ctacggtcac ttcttgcttt
    16741 tctaaaactc gtttgacata atttcccatc ccagaattga tctcatttgc gccagaaggt
    16801 atttcaggaa acgaaagacc taaggcgagc tgcagtgtat gaatcccttg ttcttttgtt
    16861 ttttctgcaa gatcttctat cgtttggact tcggaaagat catgtcctct aattgtaatg
    16921 ttcaacataa ctattctcct aactcagcat attgggtagc cacatgacga tttgcgggaa
    16981 gtaagtaatc aaaaacaaga cgactaagat aaccccataa aaaggaagaa gcggtttcag
    17041 cattgcctct gcttttacgc ctcctacact tgcaccgaca tatagtcctg tacctactgg
    17101 cggcgtgatg gagcctacac acagattcat gattgaaaac aacccgtaat gtacaggatc
    17161 aacccctaca cttgtgacga ttggtaaaaa tattggtgta aaaatcaaga tagcaggcgc
    17221 cacatccata aacataccca ccagtaataa cacaagattg actaataaca gaataataaa
    17281 tttattatcc gttacgccca taatcaacga actaattgcc tgaggaatac cggtaaacgc
    17341 catggcgaaa gacatcatgg aagaagtagc caataagaac atgatcgtcc ctgacattgc
    17401 tactgattga aacaacatat taggtatatc ttttagtttt accgttttat agtaaaacat
    17461 agcaatcaac agggagtaaa tgacagctat cgctgaagct tctactgctg taaatatccc
    17521 cgtcaagata ccaccgataa tgatgacaat caatagaagg ctaggaatcg cctcttttat
    17581 tatttttcca gcatgagcca tctgtccttt ttcgacaaca gggtatttat tctttttagc
    17641 aacgatgaat gctactagca tgattcctag cgcccacaaa gcgcctacga gataacctcc
    17701 cataaacaaa gcagagatcg aagcaccacc agcaacaagt gaatacatga taaaagcagt
    17761 acttggagga atgaccatac ctgtcggagc tgacgcgata ttgaccgctg tcgcaaactt
    17821 ccggtcatat ccttcttcca cttgctgagg gatcaacact ccgccaatcg cggtagaagc
    17881 ggcaatggcc gagctagaga tggaaccaaa taaagcattc cctaacacat tcgtatgagc
    17941 caaagccccc ggcacgcgcc cgacgaacaa catcgcaaaa tccactagtt tcttagctat
    18001 ccctccatta ttcatgataa tgcccgagag aacgaaaaac ggaatagcga tcagcgaaaa
    18061 actattgata cttcccacca ttttttgagc tgaactaaaa gcagaaacat caaacggtat
    18121 aactaacaat aaagtcatca tcgatgagaa tgcaatactt atagcaatcg gcatccctaa
    18181 tacaagcaac acaacaaata caaaaaataa cactaatccg gcttctattg ccattttatt
    18241 cctccatttt tatatttttt cttgaattgc tggcttgttg cttaatgtat cgattagatt
    18301 gatcaggcta tagataatga tgagtacccc cgatacaggt actgacagat aaacatatcc
    18361 cataggaata cttaaagaag gcgagatctg ccccattgtc aatgtgacag cattccatcc
    18421 gccatacacc atgatcatcg cagcaaatac cagaaataaa ctttgaacaa caacttctaa
    18481 aattttactt tttttgccag ttaatttttc acttaaaacc gttacggcta aatgtgcttt
    18541 ttgtcccaca acatacgcgg aagacaacat ggcaaaccag acaagaccga atcgaacaag
    18601 ctcttccgtg accgtatttg gatttcctaa aaccgttctg gaaaatactt gccataaaac
    18661 aacagcgacc atcccaacaa gcaaaacagc tcccaggatc tctaatacct tgtttaaaat
    18721 cattttcatc acagctacgc tccttgctct tggatctttt gataaaggtc tttgtaggta
    18781 ggatcgtttt tgaatatatc atgaagtggg gcaactgcct ctcgaaacgg tgtattatct
    18841 acatcattga attggactcc atgttcttct tgtgctaaag ctttttcttc ttcaattgct
    18901 tttgcaaaag cttcaatttc aaaaaccgtt gattctttcg ctgcttcttc aatggctttt
    18961 tcctgctcat cagttagctg ttctttgatg gcatcattca tgatgatgat atctggcaca
    19021 cgtgtatgct catcatagga atagtattta gccacaccac catgtccagc tgtatacaag
    19081 acaaattcat tgttttctga tccgttgatc aaattggatt gaagagacgt gtacacttca
    19141 tcacttccca taggtacggg cgaaccgcct aaaagctcta ccattttgat agcggtttcg
    19201 ctttgcatca cacggatctt ctttcccttc aaatcttccg gcgtatggat cggaccgtct
    19261 accatataaa agctgcgctg accagaatca taataggtca atccggtaaa tcctaaattt
    19321 ttggtcgatt ggtagattgg ctccattatt tcttcattat ccatcacctt aaagaaatgt
    19381 tcttcatcat tgaatagata aggaactgaa aaaatcgaat aatctttaga gaaactttcc
    19441 aacgctgagg cacttacttt tgtgaaatct actgctcctg actgtgtcaa ttcgatcagt
    19501 tcacgttctc cacccagttg tccatctggg aaatactgga tctgtacgga accatctgtt
    19561 ttttctttta ctaaacgccc aaattcattc attgcatcaa ttactggctg gctgttgctt
    19621 gcataagcaa agcgcaaggt aactatctca tttacatctg catctgcgac agttgccaca
    19681 ctagaattat ttccgcagcc gcttagacct agcagaccta ggctaagcga acttgctgca
    19741 aacagcagtg atgttttttt catatgggtt tcctttcttt taacaataag agacgagcaa
    19801 ttctgtcaaa caaagcaccg ttaacgactg tccataaggc attgctgtga ttccgatatt
    19861 ccgataaaaa tctaaactgt cccccattcc tgtcccgatt gaaacgtttt gaacttctcc
    19921 tttttcatcg atttgttcaa gcaatccttg gatagcacga taagcgactt ctttatattc
    19981 ttgtggtaaa taacgtttat ggaccgcttt caaaataccg taagcaaatc ctgcagttgc
    20041 agaagattct acgtaagaag aaggatcatc caacaacgta tgccataaac cgctttcaga
    20101 ctgataagtt tttaatgcac gcacttgtgc ttcaagtgtt tcgatcaata atttgcgcaa
    20161 tgcatcttct ttcgataatt ctaagatttc aatgatctct ggaatagcaa tcgtcaacca
    20221 gcagtttcca cgagcccaaa atgcttcggc atagttatga tttccttcaa acgtccagcc
    20281 gtgataccac aatcctgttc gtttatccat caagtatttg atatggatca taaactggta
    20341 acgagcttct tctaaatatt ctgggcgatt caacagtttc ccgatttttg ccaaaggaag
    20401 cactgtcatc atcaatgtat cgtcccacaa ctgattctta ttttccggtc catatgtggc
    20461 atgctgtaac ccgtcttcat ttgtccgagg catgtcattc atgacccatt ctgcccattg
    20521 ttctaaataa ggcaaatagc gtgaatcttt cgtgtcttca tacaagtaag ccatagtcaa
    20581 tagcggtgcc attgtattga cgtttttggg cggagcgcct tcttccatac gatcctcaaa
    20641 ccattcattg accacttgat aagcgcgagg attttttgtc atctggtaat ttttataaat
    20701 accatataga ccgacacctt gtggccaatt ccacactgtc cagcttttgt catctacttt
    20761 cagtccatca aagtccaaca aaaattcacc attatcgtct ttgatcgttg tcaggttttc
    20821 aacgagcagg tcaatttttt ctttcactaa ttctttaggt acaaaaatct cttctttaat
    20881 aatcatcttt acgccacctt tttaaacgct ttcattttat atccttaata tagcagtaat
    20941 accgtccact ataaatatcg ctttttgaac aaaaactgtc taaaattgac ctaaatgtta
    21001 aataacaaaa gtaactttca gttacttgga aattaaaaaa agaacttgga gaaacaacgt
    21061 tttccaagtt ctttttcatc tattttctta tcttttttct acgctttctt ttgcttatcc
    21121 gttgtaagca tcaatgatga tctgtttcaa ttcagagatc aatggttctt tcggattagc
    21181 tgtcgtacat tggtcttcgt aagctaattc agccatacgg tctacggttg tatctagtgt
    21241 ttcttgtgtg actccttgag cttttagact catgttgatg ccgacagatt ttcctaattc
    21301 ataaacagcc gttgctaaag cttcgactaa ttcctctgtt gtttcgcctt tcagacctaa
    21361 gaattttgcg atatctgcgt aatctgtatc tgcacggaaa tagtcatatt tagggaacat
    21421 cgcatgtttt tgtggatctt ttgcattata gcggataata tgcggtaaaa gaatcgcatt
    21481 tgtccgtcca tgagggatcc cgtattcccc gccgatttta tgtgcgactg agtgacaaat
    21541 acctaggaat gcgttagcaa acgccatacc agccattgta gaagcgttgt gcattttttc
    21601 acgtgattcc atatcaggtg tttttactga tttttctaaa tagtcaaaga ctaacttgat
    21661 tgcttgtagg ctcaatccac gtgtataatc tgaagccatg actgaaacat aggattcgat
    21721 tgcatgtgtt aacacatcca tcccggtatc tgctgtgaca gatgctggaa ctgacatgac
    21781 gaattgagga tcgacgattg cgacatctgg cgtcaatgca taatctgcta acggatattt
    21841 cacatgtgtt tcactgtcag tgatgaccgc aaatggcgtt acttcagaac cagtccctga
    21901 tgttgtcggg atacatacga attgtgtttt ttctggtttg tcgatcttat atgtccgttt
    21961 acggatatcc aagaattttt gttttgcacc aaagaaagat gtgtctgggt gttcatagaa
    22021 catccacatt cccttagctg cgtccattgc agaaccgcct ccaagagcga ttactgtatc
    22081 tggcttgaag tctgccatca gtttcgttcc tgcgtatacg gtatttgtcg atgggttcgg
    22141 ttcaacatct gagaagactt cgatcttcac atcattttta cgtcgtgcca atacttcacg
    22201 aactgtatcg cagtaaccga attggaccat accagggtca caaacgatca tgacacgttc
    22261 aacgttttcc attttctcta agtacaatag agagttcttt tcaaagaaga tttttggcgg
    22321 taatttgaac cattgcatgt tatttctccg tttcgctaca gttttgacat tgattaagtt
    22381 tacagcagag acgttacgag aaacagagtt cttaccataa gaaccgcatc ccaaagtcaa
    22441 tgacggaatc atttcgttat agatatcacc gatgccgcct tctgcagatg gtgtattcac
    22501 taaaatacga caagctttca ttcgaagacc gaatttgaca tgcaactctt cgttttctga
    22561 atggataact gctgtatgtc ctaatcctcc aagattcaac ataccttcac acaaatcaaa
    22621 cgcatggtcc gtgttgttag ctttgatcat ggcaagaact ggagaaagtt tttctcttga
    22681 tagcggataa tctgggccta cgccttccaa ttcagcaatc agcattttcg ttccttttgg
    22741 tacactgatt cctgccaatt ttgcgatttc catcgcagaa tgtccaacga ttgatggatt
    22801 gaccgcatat tttccttcgt tcatcactgc atcttctaat ttttgcaatt catctggttt
    22861 tacgatataa acttggtgtg cttggaattc tgcttttact gctgcataga tttctttatc
    22921 cacgatcaca gcttgttcag aagcacaaat cattccatta tcaaatgttt tcgaaacgat
    22981 caaatcgttc actgcacgtt taatttttgc tgttttttca atataagctg gtacgttacc
    23041 tggacctaca cctaaagctg gttttcctgt agagtaagca gacttgacca ttccagctcc
    23101 acctgtcgct aagacgatcg cgatacctgg atggttcatt agcatagaag tcgcttcgat
    23161 cgaaggatgt tcgatccatt ggatacaatt ttcaggagct ccggcagcga tagcagcatc
    23221 acgtaccact cgtgctgctt ctgcagaaga tttttgggca cttggatgga aagcaaaaac
    23281 gattggattt cttgttttca acgcgatcat tgatttgaaa atcgttgtgg aagtcggatt
    23341 cgttgttgga gttactccgc aaaccacacc gactggttca gcaatttcaa tcaacccttt
    23401 ttggacatct tcattgatca caccaactgt tttatcatgt ttgatattat gccaaatata
    23461 ttcggacgca tacatatttt tgattgcttt atcttcgtaa acaccgcggc ctgtttcctc
    23521 tacagccatc ttcgctaatg gcatatgttg atccaaagct gccattgcca tttcatgcac
    23581 gatatggtct actttttctt gatcaaagct ttccatttct ttcaatgctg catttgcttt
    23641 ttctgccaaa tcatcaatca ttgcttgtac gtcaattgtc ttcgtttttt ctttttctat
    23701 tactttagcc atcattatcc tcctaatggg tcatggttat ttgttaaata attcacatgt
    23761 atatcataca atatttattt ttctatgtaa acactttttt gtgatttttt tcacagttat
    23821 ttttaaatat aaaattgctt tatttacagg tgtttgtata gaaaaatata taaagttatt
    23881 tattattatt tatatgacac acccgattat tcaatattga gcaatatatc ccatgctctt
    23941 atctctttct tttttgaata aaaaatgagg taggacatct ttctgcccta cctcgtcttt
    24001 aaataagctt tgatacttat ttttcttcct tctcttctgt ctcttccagc gtttctgttg
    24061 tttcagtttt tacttcaact gtctcagctg gttcttctgt tgtttcaaca gtagtcgttg
    24121 catcatttgt tacgacagtc cctggtttga ccgttttgat tgctgcacga tcgtactcaa
    24181 ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc attgtcgatt tctgaaacaa
    24241 ctccatgtaa tccgccgatc gttacgactg aatcacctgg tttcatgcta tctaacaagt
    24301 tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat aaaaaaccac attgctaata
    24361 gcgcgataaa catcacaagc attggtaatg ttcccatttt tttcacctca ctaattctgt
    24421 atctacaaaa aacactttag cagaaaaatg ctgataacac tagtttttta cttaattttt
    24481 aaaagatttt cctttagaag ctttttgcgt tttctttatt gaacccgtat tcttcaaaaa
    24541 aggcttctct aaattcaagc aaattatcat ccatgattgc ttgacgtact tgtttcatta
    24601 gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg aatcccaaat gtttcatcac
    24661 atttgatcaa atggcggata taggcacgtg tatagttttt acatgtataa caatcacatt
    24721 tctcatcaat tggacggaaa tcatgggcat atttcgcatt tttcactact agacggcctt
    24781 gagaagtcat acatgtgcca tttcgagcga ttcgagtagg caagacacag tcaaacatgt
    24841 ctactccacg gataacacca tcgatcaatg aatcagcagc accgacaccc atcaagtaac
    24901 gtggtttgtt ttcggggatc aacggtgtag tgaattctaa tacacgattc atttctgatt
    24961 taggttcccc gacagataac ccgccgattg agtaaccagg gaaatccatc ccgactagat
    25021 ctttcgcgct ttgacgacga agatcttcaa accctgctcc ttggatgatt ccaaataatc
    25081 cttgacggtc tggattagca tgagctttca atcctcgttc tgcccatcga gacgtacgtt
    25141 ctactgattt tttcacgtag tcgtaacttt catcgaatgg tggacattcg tcaaaactca
    25201 tcatgatatc agagcccaat ttattttgga tgttgattgc tttttctggt gataaaaaca
    25261 tacgcgaacc gttcaagtga tttttgaaat gcacgccttc ttcttcgata ttccgcatat
    25321 cacttaatga aaaaacttga aagccgcctg aatctgttaa aatcggttga tcccagttca
    25381 taaatttatg cagtccgcca gcttcttcta ctaagtcttc accagggcgt agccataaat
    25441 gatatgtatt acttaaaata acgccagcac ccatttcttt caactcttct ggggacattg
    25501 tttttactgt agctaatgtt cctactggca tgaacattgg tgtggggaaa gttccgtgtg
    25561 gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc tttcttgatt aaacgatagc
    25621 gaatggcagg ttgcgtcatt catttcccta ctttctatat aaaatatctt gacaactccc
    25681 accatcataa tcgtttcaag tgctttttgc aagttccctc ggcttccttc tactttattt
    25741 aatattcttt tttttatcct ttttttatga ataacaaaaa aaagaaaaac attatgacag
    25801 aaatattttt tgatacatgg tatattggtg ttattaaaga aaaggaggga aaaatcatgc
    25861 aatcttctgc agaattaaaa cgccaagcaa aagagagtct aaaaggacgt tggggacaag
    25921 ctgttttact aaatctaatc cctacattga ttggtattgc tactattctt cttattatga
    25981 ttcctattat cttgtttgcc accaccatat atgatggatc agcgagcgtc tcagaagcga
    26041 caacttcagg aggcggtagt tcaggaggcg gtggcggaat tatttccagc atcattagcg
    26101 ctttgttcat gagcggtatt tcttggactt atcttgatat tttacgtgga caaaaagatc
    26161 agatcgaacc attcaaagat gcatttcgtg gattcaaagg tttgtttatc ggcggagtga
    26221 tcagcttagc tgtgctgatc tcgatcttta caacattatg gggtttgcta ttagttattc
    26281 ctggaatcat taaaagctat tcttattctc aaagctattt tatttactac gatatcgtcc
    26341 aagaaacagg acaaacacct aaagtactgg atacgatcac tgcaagtcga aaattgatgg
    26401 atggttataa aggacaactt ttctggttag atctttcatt tatcggctgg catatcttag
    26461 cattagcaac tgctggtatt ggttatctat ggctcagccc atacatttct gcaacaaaag
    26521 cagcatttta tgaacaattg ccaaaagata tttaaaagag agtgtgactt gtgaccgagg
    26581 gggtggcagg agaacaccgt ttatcgagag gcagtaaaag aaacattcag cccattagca
    26641 aaaaggaggt gtgacaacat tcatgtcaca cctccttttt gtatagttcc agtaaaaaga
    26701 aaatcaactt agaaacagtt aatcattcct cttcttctgc ttccggtaat cgatggaact
    26761 tgatcagttc attcaccgcg attggtacga tagataaaat ccctaccaag aaccagtgat
    26821 tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa tggcagcaaa gcaacagccg
    26881 ttgtaatagc taatgccagt aacgccattt ctaccaaagc tttattggtc gctaaattga
    26941 ctttgaagat cgattggctg ctgcgtacat tgaacacatg cagaatcgat gaataagcta
    27001 agatcaagaa tgcaacagtt tgaccgacat gctgacttgc gtctacccat gggctgatag
    27061 tatcgacata actacctaaa taataaccaa acaaggttac gatggtgaag acccctgcat
    27121 ttatggcaat tttttgcaat aaacctcgag caaagatccc ttcatttttc ggaatgggtg
    27181 gctgatccat gatgccgtgt tccgctggct ctcttcctaa agcaaatcct ggaagtccat
    27241 cagcaactac attgacaaac agtagttgca cagctgtaaa tggtgctccc catcctaaca
    27301 gcatcgcaat caaaacaata aagatctccg aaatattaca gcttagcaaa aagttgatag
    27361 ctttacgaat attctgataa accgaacgtc cttgactgac agcgtctacg atcgtcgcaa
    27421 agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc cacatctgtt cctgtgatcc
    27481 ccatggcaca cccaacgtta cttgctttca acgctggtgc atcattcaca ccatccccag
    27541 tcatcgctac gactgcaccc gtacgttgcc aagatttgac gatccggatc ttgtcttctg
    27601 gcgttacccg agcatagaca gagagagctt tgacacgcgc atctaattct tcgtctgata
    27661 attgatgcag ttctgatcct gaaagtgctt ctgacggatc attcaagata cctaattctt
    27721 tcgctatggc actggcagtc accacatgat ctccagtgat catgactgtt ttgatgccag
    27781 cctttttcgc tcgcttgatt gctcctttac tttctggacg tggtggatcg atcattccga
    27841 tcagaccaat caaacgtaag tctttttcca gtgcttcaga agaaatatct tgcggttctt
    27901 cgtcataagt agcataacca acagcaatca ctctcagtgc ccgtttacca aagcgatcat
    27961 taacgatcgc cgcttgttct acatcgcctg aactaaagcg cggagcaaga acatcgaatg
    28021 ctcctttggt gattgagatg tatttttcgc ccatttggtg aactgttgtc atcatttttc
    28081 gttcagaatc aaaaggaagc tcgtttactc gtggatattt ttcttctaat tctgcctttg
    28141 tatgataatt ctcttcaaca gcccttacga tggctacttc tgttggatta cctttgataa
    28201 caaggtcgtc atcttcttgc tcgaccgtca catctgtaca tagtgcagcc attttcaata
    28261 tttccatcgc gctgttcgtc attgaatctt cgatattcgt tacttcatct tctttgctcc
    28321 atacccggcg gacgcgcatt tcattttgtg tcagcgttcc tgttttgtct gagcagataa
    28381 cattagcggt tcctagcgtt tcaactgctg gcaactgtcg gatgatcgca tgtttcctcg
    28441 ccatcttctg cacaccgtat gcaagtgtca acgtaacaat aacagtcaag gtttccggta
    28501 cagctgcaac agccaaggaa atcgaagtca tgaacatctc aaggagtggt tctccttgca
    28561 gttcaccaat cacaaatact aaagccgcag ccgctaaagc gatcatactg attcgtttgc
    28621 ctagctgatt caatcggacc tgtaaaggtg ttttttgcat agtattttcg ttcaaaagat
    28681 ccgcgatctt acccatttct gtagccatgc caatggcagt gacgatcgct ttcccacgac
    28741 ctgcagcaac cgtacatcct ttaaatacca tattaagttg gtcacctaat gactcatctt
    28801 cttcagcaat aaatgctgca tctttttcaa ctgcttcact ttctccagtc aacgccgatt
    28861 cttctacacg taattgtgat gcttcgatca aacgggcatc ggcagaaata gcatcaccgg
    28921 actctaaaac aagaatatca cccactacca gttgctctga ctcaacagtg gtcactttgc
    28981 cgtctcgtat gacagttgcc gtttgtttat tcatatcttg caaggattcc agtgcttttt
    29041 ctgcgtttcc ttcttggaca atcgccaata ccgagttgat cacaacgata gcgatgatca
    29101 gcagtccttc aaataaatcc ccatgttctg tagcaaatgc ggtataaaag gaaatcaccg
    29161 ctgctacaag aaggatgatc gttgtaaaat cggacaagct tcgaaagaat tttttgatca
    29221 tcgattcttt cggcgcctca tcgaatttat tcaatccctg cttttttcgc cgttcatcca
    29281 cttcttgtga agacaaccca gtatctggat ctgcttctgt taccttcttt acttcttcta
    29341 ttgtctgctt gtattcttcc atcctattcc tcctttaaaa tcttcgtaca aaaaaaggta
    29401 cttattttac tataacatcc tttttttatt tacttcaaat aaagggctta tcgattattt
    29461 gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa cgctaccaaa ggatgctatt
    29521 atatagatgt aaggaaggag gataccatca aatagacaga agaatttctc gatagaacag
    29581 tgtaagcaaa caaagttgtt agaagtacag gtttcaccca gacatgcgtg tagagtacaa
    29641 acctataaat agtgaatgcg tatggatgtt tggatcacga agtatccgaa acaagaagta
    29701 atgcgatacg taacaattga ataacgtaaa cgtctgaaaa ttgaatagga acaagagatg
    29761 tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt taacatgaag aaattcaaca
    29821 ccgtgctttg catgtatgat ggaaagttac aatcatttct gtttatttga atccttccga
    29881 ggtacaagaa agccgagctt atcgagagct cggctttttc tttgtcataa aatctttgta
    29941 aagctttttt gttattcaaa actagcggct tttcataacc ttatttcacg aacatcgcat
    30001 ctccaaaact aaagaaacga tagcgctctt caatcgcatg ctggtaagca gataaagtca
    30061 attctcttcc tgcaaaggca ctaactaaca tgactaaagt cgattttggc aaatggaaat
    30121 tagttgagaa agcttctacc actttgaatt catagccagg cgtaatgaaa atatcagtcc
    30181 atccgctgtc tgctttgatt tctccattaa atttcgttcc aatcgtttct aatgttcgaa
    30241 ttgaagttgt tccgacagcc acgattttcc cgccattttt acgtacttgg ttcaatcgct
    30301 cagccgcttc ttccgtcaaa cggtagaatt cactatgcat atggtgttct tcaattttat
    30361 ctacgctgac aggtcggaat gtacctaacc ctacatgtag tgtcagatag actaattcaa
    30421 caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa atgcaatcca gctgttggcg
    30481 ctgcagcaga accattttct ttcgcataga cagtttgata gcgatctggg tcttccagac
    30541 gttctttgat atatggaggc aatggcattt ctcccaatga ttcaagaatc tctagaaaaa
    30601 ttccttcata ctcaaagtca atgatccgtc cgccgtgttc caattcttct ttcactactg
    30661 cttgcaatct gccatcacca aaagagattc ttgtcccaac tttggcacgt ttcgcaggtt
    30721 tgatcaatgt ctcccacgta tctccttgtg tattcgttag aagaaggact tctaaatgcg
    30781 caccagtatc tggtttttct ccatataaac gagcaggcag aactcgtgta ttattcataa
    30841 ctagcgcatc accaggctgt aactcttcaa tgatttcatg gaaatgttta tcttccatct
    30901 ctccagtttt ccgatccaaa acaagtaaac gagaatgatc gcggtctttt aatggtgttt
    30961 gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc tgttgttaac atatcgtcac
    31021 tcactttcta tcatgtgctt catcattcta tcatgttttt cgttctgttg gcaaaatcag
    31081 cactttttca cttctatttt ttgttttcct aaataaaaaa actaccctat ttgtattttt
    31141 tcattgtttt aggcatacta gtaatagata gttcttcatt gaaaatagaa aaagaggtgg
    31201 caccatgatt gaatttcaaa atgtgtcaaa aatttataaa ggtggaaaag tcgctgtaga
    31261 ggacgtcaat ctttcatttg aaaagggtga gttcatttgt tttatcggta cgagcggaag
    31321 cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact gatccaacac aagggaaaat
    31381 tttgattgat ggaaaatcca tccaagaaat tgatccagtg gaattgcgac gtcagatcgg
    31441 ctatgttatc caaagtattg gattgatgcc tcacatgacg atccgtgaaa atatcactct
    31501 tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat aaaacagctg aaaaaatgat
    31561 tgatcttgtg gaattgcccc gtgagatgtt agatcgctac ccacatgaat tatctggtgg
    31621 tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg aatcaagata tcatcttgat
    31681 ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat tccttgcaag accttgtcaa
    31741 agatttacaa gagcgtttgg gaaaaacgat cgtatttgta acccatgaca tggatgaagc
    31801 gattaaacta gcaagccgta tcgccatcat gagtgaaggc cgtgtgatca atttgataca
    31861 ccagaaaata ttttacgtca tcctgcaaac gatttcgtag aagagttgat tggagaagat
    31921 cgcctactac aagccaagcc aaatgcgaca agagtcggcg aagttatgct aaatacagca
    31981 atcacgatta caccggaaaa atcattacaa gaagcaatca aactgatgag ggaaaaacgt
    32041 gtagatacgt tgttagttac cgataattcg aatgtattaa aaggatttat cgatgtagaa
    32101 acgttgaata aaaaacgagg aaaagtatcc agtgtcggcg acattttgaa taaacatgtt
    32161 ttttacgtaa aagaatccgc ctatcttcgt gataccttgc aacgcatttt aaaacgcggg
    32221 ctgaaatacg tgccggtcgt tgatgaacag aacaaagtcg taggtatcct gactcgtgca
    32281 tcattagtgg atatcgtcta cgatgtgatt tggggagaag atcccgcaac tactgatgtt
    32341 gcagagtcag ctactcctga agaatctaag gaggtctgac ctatgcagca gttttttcat
    32401 cagcacggtt cagaaattct ctcaaaaggt tttgaacatc tttatatttc aggacttgct
    32461 ttgatcattg gtatcatcat tgctgtaccg cttggtgtct tactgacacg ttttccaaaa
    32521 gctgccacga ttgtgattgg cttaaccagt gccttgcaga caatcccttc tcttgcttta
    32581 ttggcattga tgatcccgct atttggcgta ggtaaattac ctgcgattat cgcactcttt
    32641 atttattcat tattgccgat tttacgtaat acgtacattg ggatgaaaaa tgtcgattgg
    32701 aattatcggg atgtggcaaa aggcatgggg atgactgagt tccaatccat tttttccgtt
    32761 gaattgccca ttgctatgcc aacgatcatg gctggtattc gtttagctgc cgtttacgtg
    32821 attgcatggg ctacacttgc ctcttatatc ggcgctggtg gtttaggcga tttgattttt
    32881 agtggactga ataattacca accagatctc attttggcag gtacgatccc tgtaacaatt
    32941 ttagcactat tggctgattg gcttctaggt ctcttggaac accggctgac accgatcgca
    33001 cttagagagg agaatgaaga atgaaacgta tactaaaaat gaccatggtt cttttctctc
    33061 ttcttatact tgcgagttgt tccttccctg gtcttgccag caatacagat gaagacacca
    33121 tttcgatcac aggtggaatc acttcagaag cacagatctt agcaagtcta gttgccggaa
    33181 tggttgaaca ttatacagat aaaaataccg caatcatcaa caatctggct actacaacta
    33241 tcaaccatca agcgatgatg aacggagatg cttcaatttc agcggctcgc tacacaggaa
    33301 cagatttaac gactacattg aaccttcctc ctgaaaaaga tccgaaaaaa gcgtttgcta
    33361 ccgtgaaaga tgaatttgag aaaagatacg gtcaaacgtg gttcccgtcc tatagttttg
    33421 aaaatacgta tgtctttctt gttcgaaaag acaccgcaca aaaataccat ctatctaaag
    33481 tcagcgatct gaaaaatgta gcggatgaat tagtagctgg tgtagatact tcttggatca
    33541 atcgtaaagg agacggttat gacggcttcc aagaaactta tggcttctcc ttcaattcga
    33601 tattacctat gcagattgga cttgtttatg acgcagtcga agcaggaaaa atggatattg
    33661 ttcttggcta ctctacggat ggacggatcg ccagttatga tctagtcatg ttggaagatg
    33721 atcgtcaatt cttcccacca tatgatgctg ctccagttgt agacaacaag atattagctg
    33781 atacgcctca tttaaaagaa gccttaaata agttaggtca taccatcagt acggaaaaaa
    33841 tgcaggaatt gaactacgaa gcagataata atctagtgga accatcagta gtagcacaac
    33901 gtttcttaca agaaaaccac tattttgaag ggaagtgaag caattggata aaatgaatgt
    33961 ctttcaacaa tttttctatt atttccagga aaatggctcc tatatctttg cacaatttat
    34021 ccgtcacttc ctgatttcta tctatggtgt cctctttgca gcagttgtcg ggattccggt
    34081 tgggatcatg atttctagga gacgtaaatt agcaaactgg gtgattcgtt tggcgaatat
    34141 catccagacg atcccttctc tagccatgat ctccatttta atcattggat taggcctggg
    34201 agtcaatgtc gtgattgtta ctgtattttt gtattccctc ttaccgatca tcaagaatac
    34261 gtataccggg atgatccaag tagacaaaaa catccttgat gtcggcaaag gcatggggat
    34321 gactgctaga caacgactat tcatggtaga gttaccactc tctgtttctg ttatcatggc
    34381 aggcataaga aacgcgttag ttgttgccat cgggataacc gcgatcggtg cattcgtcgg
    34441 tgctggtggt cttggagata tcattatccg aggaaccaat gcaacagagg gcacatcgat
    34501 tattttagca ggtgcattgc caactgcatt aatggcgatc atcacggact ggcttttagg
    34561 tattttggaa agacggctag atccagccag ccgtacatca cgctgatgtt attgtaaaaa
    34621 acaaaagctt ctgcgaccat ttgttggtcg caaaggcttt tttagttctg tcatttttaa
    34681 ggcaataatc cactctgatt cttccctcct tatgggcaaa gagttgcttt tcttagctat
    34741 ttatccattc cgtatactcc aacttcatac tgccaacggg ttcatatctg catccaccga
    34801 ctaagttttt ttcttatccg ctaaggcatt ttgccaatcc attttcccat cactcgtcat
    34861 ggaattcata ccacatgatc tctaaatact cgtctgcttg ttctttgaat tgggcatcaa
    34921 ttcttacatc ctttggcgtg gctttcattt aagccttttc catcagtcct cgattgttcg
    34981 atgccttcag ctgcatcatc aaaactggtg actgccgtat cggtaacttc gatcatcttt
    35041 tatacaatcg ggaacttgcc atcctcatcc ttggctaaaa acatatctag ccattccagc
    35101 ttctcgccat gggaggtttg cggttcctaa aaccggtaca cgattcttat ggactgcatc
    35161 cgtcgcaact agacttggcg atacgatgat ccttcaccag aagagcaccc ccgtaaacca
    35221 attgatcgat atattgccaa taagaaaaga cattggcgta aaagatatcg atccctcgtg
    35281 atgcattgcc gttgatactg ctattcatgg ccgaaagcgc cacgacttta acttcttccg
    35341 tttgagtgac attcatgttc ggcaatgtcg ctttggatac ccgctctgct aaaggaacga
    35401 cactggtatt gtatttcgta tctgtatctt ttccaaaaga ccatgccaac gaatcctctg
    35461 ggaaccaata agacgactcc ggttgattgt ccattgcttt tttcaacata cttttggctg
    35521 tttctcgata aacgacattt ccaattgtct tcgcctcact ttggcgataa tcgatggtcg
    35581 ttgccactgc caacactaac aacgatgctc caattttact gattttcctt gtcacacttc
    35641 ttcctccttg ttttacataa acccgtcaac attccattcc taaactcaaa caatgaccct
    35701 tcaaaggtgg agatatagga acaaattaaa gaaatcatgc aataaatcca agaaggttgg
    35761 ttttagaaca aaaaaagagt ctcctttttg gggagactct ttgttaacta tctaaaaagg
    35821 ttggctcttc tcacttcact cataatgtgt ccaggcagaa taaatttcta caactttatt
    35881 ttcctcatca actttgtaaa ccactcgatg ctgtctattc agcctgcgtg agtatctgcc
    35941 ttcatgtgta ggtctcagct tttcaaaaga ctgcgtgggc atatatggat cttccttcaa
    36001 tgtctgaatc acttcttcaa attgtttctt taaattcgtt tgcttgattt ttcttaaatc
    36061 cactttcgca gagtttttga ttgcgactgt atagttactc atcaaagagc ttcccaatca
    36121 atatcatcga tattcgtaaa gctactgtcg tctttttcgc ggtctcgtac tttatctaaa
    36181 gtacctgttt gctcaagaaa aagggtttct tgaatcgctc tccagtcttc cagcccaatc
    36241 aatacagcat tgtttccact acgatcacta ataatctcga tttcagtatg attttcattt
    36301 acctctttca ataactgata aaaattttta cgagcagtgc taggattcat cgttgtatta
    36361 gccattgtct ctcctccttt ttctgatata attatagcgt acttgattaa gtacgtcaaa
    36421 aaaatacaat aaagcactta aattcagcaa acttttcctc ttttttacat agagaaaaag
    36481 accccttttt agggaggatc tttgtccacg ctcacttctt actcctttac gactagcggt
    36541 agccaaacgt aaagtaattg gtaacaacgt atcatcttta gcaaaggtcg aatccaaagc
    36601 ctgtgagtct atgcaacaac accaatacac gaaggttcct cgttgcagtg tgacttgatt
    36661 gatatcttga ataacctgag gatacgcacg aaagatttgt aacggttatt caaaactgat
    36721 tttggccgtc aagtcttcta ctgcctcaaa aatgattgcc tctttcgtct cctgtcattc
    36781 atgttcacta tcgatttgat acttgcttag accgttgggt ttgtgaatca ccaattaaaa
    36841 atggcactga gttatggtaa caaaaaatgg tcacttgacg aggctgagat tcctacttat
    36901 gaagacacat ggcacttaag agtcgaggta aaggaagaag ctgaaaatat aggaggctgt
    36961 acattatttg gcaaaggatt cggaaattat gatcaagaca tcaaaatcaa aatgttttac
    37021 agctaaatag gaagaataat aacatttcat acgctgttcc catctttaat ggcttataga
    37081 gggcaccttt agctgagaat aagacaaaac agaccctcta gcttcaatta agagctagag
    37141 ggtctgtttt tgattttaaa acttggttta tttcttcaaa tggtagctat aggtgctgac
    37201 aacgatattt tcacgccaag acaatcgcaa tcttaaccgc aaacttgttt ggttatggag
    37261 aatattctgc cagcgtctgt ttggtacaaa ttgaggaatc aacacagtag ttgtatagtt
    37321 ttgtttcgct gcatttttac ttacgatatc tacataacgg ataattggat tttcaatcga
    37381 gcgataagaa gagtggacga ttgaaaaacg gacatctggg aaatgctttt taaattctgc
    37441 ttcgatttct ttttcttttt cgacatcttc gtctagcgaa acatgcatcg caacgacata
    37501 atcaccgatc gaacgtgcgt aattcaacgc accgatattt acccgtgtca cattcccgac
    37561 taatacaaga acggtattcc cgtcataatc atgtagtttt gcatcatttt ctaaacgaag
    37621 ctgttctgct acttttttat aatgatcatg gatcttataa aaaatgaaca taacgattgg
    37681 cataatgatg aagaaaggcc aaatgtcccc taaccgatag acaaacaaaa tcgcaatgat
    37741 tgcatatgag atgaacgcac ccgtaatatt ggcaatcgat ttgcttaacc atctttttcc
    37801 ttcttttttc catttgacga ccatccctgt ttgagataaa gcaaatggaa tgaacacgcc
    37861 gatagagtat aacggaatca agcgttctgt tgagccatgg aaaataaaca gcaatacgat
    37921 cgaacctagc gctagtgtga taatcccatt ggaatagcct agtcgatccc cacgatcttg
    37981 atacatgtga ggcatgaatt tatcttttgc cagattgtat gcaagaactg gaaaagcaga
    38041 aaaaccggtg ttagcagcaa ctgctaaaat cagcgctgta gcaaattgaa ggacgtaata
    38101 caaaatacca tgtccaaaga cagctttacc aatttgagac aaaacagtga cttctttttc
    38161 aggaacgatc ccataccaat agttgataaa tgtgatcccc acgaagaaga aacctaagat
    38221 caatgccatc atcgtcaatg tagcagcggc attttttgct cttggcttct taaagaatgg
    38281 tacagcatta ctaatcgcct ccaccccagt taaagaggaa gaaccagaag aaaatgctcg
    38341 taaaatcaaa gcgatagaaa cacccggcac agccgctcct ggaagtgcag tagcttgcaa
    38401 tggcacagca cctgtcacga tattatataa accaacaacg atcagtaacg agatcacaag
    38461 gataaatgtg tacaccggaa ataataggaa cgatgcagat tccctcagcc cacgaagatt
    38521 cagcatcatc agtagcaaaa cgattgtcac agaaatagcg acttgatgac cgtagagtgc
    38581 tggcaccgct gatgtgatcg cttcagcacc tgctgagaca gatactgcta ctgtcaacat
    38641 gtagtcgatc aataaagagc caccggaaat caagccagca ttctttccaa gattctcact
    38701 gctgacgaca tatgctcccc cgccatgagg ataagcatgg atgatttgac gatatgatag
    38761 cgttaatgaa atcaataaaa taataacaaa agcggcaatg ggtagcgaat accagatggc
    38821 tgcagcagac aaagcgacta aaacgacaac gatttgttcc gtaccatatg caatagatga
    38881 taacgcatca gacgacaata atgccaaagc agcaaatcgg gttaatttgt gctcatcatt
    38941 ttcagcagat tttaaaggct tacctactaa caaccttttt aagtaatcca atttctaaac
    39001 tcctttacac acaattttac ttcaagtttt atagtcgaaa atttatagta aacaagatgt
    39061 tatagtacgc tcactgacct taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac
    39121 agaaaaatat caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta
    39181 tgggcttttt ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc
    39241 tatccttttt gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat
    39301 tcttactact cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc
    39361 ttgcatctgc ggtaataagc ccaaccaaat taaaaacata agaagctgtt tttatttggt
    39421 cgttcttatt acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat
    39481 cagctgaccc tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt
    39541 tttttctcta atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat
    39601 atggctctca tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat
    39661 tgccgttcct acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt
    39721 agcatttggc caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa
    39781 tggcaagaac ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc
    39841 gcctccgcca gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc
    39901 gaataatgca gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa
    39961 tgacacgctg tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt
    40021 aacgatcaag ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg
    40081 ttctcgtttc gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac
    40141 acttgaaaga agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc
    40201 tgaaccgtta tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa
    40261 gaagtcactt tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac
    40321 ttcttgttcg ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg
    40381 ccagtcattg ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt
    40441 ttcatttact gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg
    40501 gataaaatca gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag
    40561 gtctgcccct tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt
    40621 ggcatcatgg atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc
    40681 cactgcattc gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc
    40741 cgctttgctc agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga
    40801 agtgacagtg gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt
    40861 aacaacggca tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac
    40921 agctgtgttc aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt
    40981 tagctgatca ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag
    41041 gaactcgacc agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt
    41101 caatacattg ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt
    41161 tcttacagta tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt
    41221 aacatctaca ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt
    41281 tgtttggttt gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc
    41341 ttgtacttgt tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt
    41401 ctcttggatc gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat
    41461 ctctgggatt ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat
    41521 tttgtcattc aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac
    41581 gaattcattt gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag
    41641 gacttgtttt gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc
    41701 tttaacttta ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac
    41761 tttcaataaa gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga
    41821 acttgctcct ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata
    41881 ttcgattttt ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc
    41941 ctttggcaag taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc
    42001 ttttgaatca acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg
    42061 ctccccaatg gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc
    42121 aattggcagc tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc
    42181 atatagcgaa gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa
    42241 aatccgtttc cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct
    42301 cccccttctt cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg
    42361 gcttttttca aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc
    42421 ttacatattg agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa
    42481 agataatttt atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt
    42541 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
    42601 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
    42661 gcttggatga catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct
    42721 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
    42781 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
    42841 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
    42901 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
    42961 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
    43021 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
    43081 ccgaccgcac ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
    43141 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
    43201 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
    43261 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
    43321 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
    43381 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
    43441 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
    43501 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
    43561 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
    43621 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
    43681 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
    43741 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
    43801 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag
    43861 atgctcgtcc agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca
    43921 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
    43981 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
    44041 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
    44101 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
    44161 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
    44221 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
    44281 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
    44341 tattcctgtt ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt
    44401 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
    44461 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
    44521 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
    44581 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
    44641 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
    44701 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
    44761 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
    44821 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
    44881 ttccttcacc tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg
    44941 gctgcaatgg ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta
    45001 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
    45061 tgacatgtac atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt
    45121 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
    45181 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
    45241 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
    45301 tctctagctt acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg
    45361 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
    45421 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
    45481 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
    45541 gaccgtatcc gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
    45601 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
    45661 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
    45721 ctgtttcatt tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca
    45781 caacaggctg accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
    45841 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
    45901 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
    45961 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
    46021 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
    46081 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
    46141 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
    46201 cgcgtttttt atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat
    46261 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
    46321 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
    46381 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
    46441 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat
    46501 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
    46561 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
    46621 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
    46681 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
    46741 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
    46801 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
    46861 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
    46921 attcgtctac acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat
    46981 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
    47041 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
    47101 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
    47161 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
    47221 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
    47281 taatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
    47341 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
    47401 cgcgccctaa acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga
    47461 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
    47521 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
    47581 ctagtggaat cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg
    47641 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
    47701 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
    47761 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
    47821 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
    47881 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
    47941 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
    48001 atttttattt catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc
    48061 cttttagcat aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat
    48121 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
    48181 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
    48241 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
    48301 acacagcgat tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca
    48361 tggctgttgg atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
    48421 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
    48481 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
    48541 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
    48601 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
    48661 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
    48721 cgtctgatac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
    48781 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
    48841 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt
    48901 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
    48961 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
    49021 tggttttacc attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat
    49081 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt
    49141 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
    49201 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
    49261 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
    49321 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
    49381 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
    49441 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt
    49501 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
    49561 taactggatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
    49621 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
    49681 atttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
    49741 agctaacttc gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat
    49801 ctgcatttcg attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt
    49861 tacgacttca ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat
    49921 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
    49981 taaagcattt tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat
    50041 attcgccggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
    50101 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
    50161 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
    50221 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
    50281 cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg
    50341 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
    50401 caagacatct ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
    50461 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
    50521 aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
    50581 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
    50641 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac
    50701 gagcgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
    50761 taaagaaaag gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc
    50821 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
    50881 caggtaaaaa aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt
    50941 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
    51001 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
    51061 tcattatcag atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg
    51121 caggctcatc gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct
    51181 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
    51241 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
    51301 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
    51361 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
    51421 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa
    51481 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
    51541 aagcttttag ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat
    51601 cctcatccag ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc
    51661 cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
    51721 tctgcagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
    51781 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
    51841 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
    51901 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
    51961 gatgaaatca acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat
    52021 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
    52081 atccgcgtcc tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat
    52141 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
    52201 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
    52261 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
    52321 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
    52381 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
    52441 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
    52501 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    52561 aaataagaag gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    52621 ttcgaagagg ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    52681 cagtaataag aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc
    52741 tttttgatgt attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg
    52801 taaaccttag tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt
    52861 aaagggtttt ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg
    52921 ttgttctaga agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac
    52981 tcgcacaccc ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc
    53041 attcttctta accataccga cgattttata atccgcacta tcagctccgc ggtctacgta
    53101 gacgatttgg acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat
    53161 aaccacgtca agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc
    53221 atctccatca atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta
    53281 ctaatccgct cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac
    53341 tttctatcgg cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca
    53401 tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt
    53461 tattttgagt agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt
    53521 gataatattc cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga
    53581 cacctatcgc aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca
    53641 attgcatagc caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg
    53701 ctgcaatcaa gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt
    53761 acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta
    53821 gaaaagtaaa cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa
    53881 caaacacgaa caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt
    53941 catcgaataa caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata
    54001 aaacaagctc tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac
    54061 gcggataatg actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc
    54121 ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga
    54181 tagctgcaaa taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa
    54241 aattgaccgt ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg
    54301 aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac
    54361 caatcagcag agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga
    54421 ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc
    54481 aattatgcga gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac
    54541 ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca
    54601 tgacaatggc tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg
    54661 ggaaataaac caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa
    54721 gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa
    54781 agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg
    54841 cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt
    54901 tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt
    54961 gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat
    55021 aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg
    55081 tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta
    55141 ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag
    55201 tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt
    55261 ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac
    55321 caatgatcgg cacaaaaact tccggccata cttcttgtat gactgccatc agctcatcca
    55381 gttcattttc tgttactttt attttttcca tacacccacg tcctttttta cattataaat
    55441 gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg
    55501 gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta
    55561 ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt
    55621 taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata
    55681 aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc
    55741 caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc
    55801 ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca
    55861 gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc
    55921 tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac
    55981 caataatggg catgatctga agacattttt gtaaaactga tcctttttta gacatctata
    56041 ttccccacat cttttttcat ttattaaaac tgcctc
//
LOCUS       DASJON010000002        49232 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJON010000002 DASJON010000000
VERSION     DASJON010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.002"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(15..1742)
                     /locus_tag="U3989_000051"
     CDS             complement(15..1742)
                     /locus_tag="U3989_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN1713532.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1735..2928)
                     /locus_tag="U3989_000052"
     CDS             complement(1735..2928)
                     /locus_tag="U3989_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1713533.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3115..3759)
                     /locus_tag="U3989_000053"
     CDS             complement(3115..3759)
                     /locus_tag="U3989_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1713534.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(3853..3987)
                     /locus_tag="U3989_000054"
     CDS             complement(3853..3987)
                     /locus_tag="U3989_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN1713535.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(3989..6121)
                     /gene="feoB"
                     /locus_tag="U3989_000055"
     CDS             complement(3989..6121)
                     /gene="feoB"
                     /locus_tag="U3989_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN1713536.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6118..6591)
                     /locus_tag="U3989_000056"
     CDS             complement(6118..6591)
                     /locus_tag="U3989_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN1713537.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            6992..7216
                     /gene="nrdH"
                     /locus_tag="U3989_000057"
     CDS             6992..7216
                     /gene="nrdH"
                     /locus_tag="U3989_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN1713538.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7375..9534
                     /gene="nrdE"
                     /locus_tag="U3989_000058"
     CDS             7375..9534
                     /gene="nrdE"
                     /locus_tag="U3989_000058"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN1713539.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9584..10549
                     /gene="nrdF"
                     /locus_tag="U3989_000059"
     CDS             9584..10549
                     /gene="nrdF"
                     /locus_tag="U3989_000059"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN1713540.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10638..11777)
                     /locus_tag="U3989_000060"
     CDS             complement(10638..11777)
                     /locus_tag="U3989_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1713541.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12086..12799)
                     /locus_tag="U3989_000061"
     CDS             complement(12086..12799)
                     /locus_tag="U3989_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN1713542.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13053..13754)
                     /gene="nagB"
                     /locus_tag="U3989_000062"
     CDS             complement(13053..13754)
                     /gene="nagB"
                     /locus_tag="U3989_000062"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN1713543.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            13988..15520
                     /locus_tag="U3989_000063"
     CDS             13988..15520
                     /locus_tag="U3989_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1713544.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15711..16913)
                     /locus_tag="U3989_000064"
     CDS             complement(15711..16913)
                     /locus_tag="U3989_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1713545.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(16995..17684)
                     /locus_tag="U3989_000065"
     CDS             complement(16995..17684)
                     /locus_tag="U3989_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1713546.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(17790..18158)
                     /gene="rplL"
                     /locus_tag="U3989_000066"
     CDS             complement(17790..18158)
                     /gene="rplL"
                     /locus_tag="U3989_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN1713547.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18211..18714)
                     /locus_tag="U3989_000067"
     CDS             complement(18211..18714)
                     /locus_tag="U3989_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN1713548.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(18918..19045)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19124..19813)
                     /gene="rplA"
                     /locus_tag="U3989_000068"
     CDS             complement(19124..19813)
                     /gene="rplA"
                     /locus_tag="U3989_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN1713549.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(19917..20339)
                     /gene="rplK"
                     /locus_tag="U3989_000069"
     CDS             complement(19917..20339)
                     /gene="rplK"
                     /locus_tag="U3989_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN1713550.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20682..21350
                     /gene="sdaAB"
                     /locus_tag="U3989_000070"
     CDS             20682..21350
                     /gene="sdaAB"
                     /locus_tag="U3989_000070"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN1713551.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21363..22235
                     /gene="sdaAA"
                     /locus_tag="U3989_000071"
     CDS             21363..22235
                     /gene="sdaAA"
                     /locus_tag="U3989_000071"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN1713552.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22326..23345)
                     /locus_tag="U3989_000072"
     CDS             complement(22326..23345)
                     /locus_tag="U3989_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1713553.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(23518..24423)
                     /locus_tag="U3989_000073"
     CDS             complement(23518..24423)
                     /locus_tag="U3989_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1713554.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24482..25027)
                     /gene="nusG"
                     /locus_tag="U3989_000074"
     CDS             complement(24482..25027)
                     /gene="nusG"
                     /locus_tag="U3989_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN1713555.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25128..25298)
                     /gene="secE"
                     /locus_tag="U3989_000075"
     CDS             complement(25128..25298)
                     /gene="secE"
                     /locus_tag="U3989_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN1713556.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25322..25474)
                     /gene="rpmG"
                     /locus_tag="U3989_000076"
     CDS             complement(25322..25474)
                     /gene="rpmG"
                     /locus_tag="U3989_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1713557.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25636..26280
                     /locus_tag="U3989_000077"
     CDS             25636..26280
                     /locus_tag="U3989_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1713558.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26336..26839)
                     /locus_tag="U3989_000078"
     CDS             complement(26336..26839)
                     /locus_tag="U3989_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1713559.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(26852..26898)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27436..28401
                     /locus_tag="U3989_000079"
     CDS             27436..28401
                     /locus_tag="U3989_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1713560.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28500..29672)
                     /locus_tag="U3989_000080"
     CDS             complement(28500..29672)
                     /locus_tag="U3989_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713561.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29659..30804)
                     /locus_tag="U3989_000081"
     CDS             complement(29659..30804)
                     /locus_tag="U3989_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713562.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(30818..31742)
                     /locus_tag="U3989_000082"
                     /pseudo
     CDS             complement(30818..31742)
                     /locus_tag="U3989_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            31859..32938
                     /locus_tag="U3989_000083"
     CDS             31859..32938
                     /locus_tag="U3989_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1713563.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            32970..33563
                     /locus_tag="U3989_000084"
     CDS             32970..33563
                     /locus_tag="U3989_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1713564.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(33673..33900)
                     /locus_tag="U3989_000085"
     CDS             complement(33673..33900)
                     /locus_tag="U3989_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1713565.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34003..36591)
                     /locus_tag="U3989_000086"
     CDS             complement(34003..36591)
                     /locus_tag="U3989_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN1713566.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36611..37270)
                     /locus_tag="U3989_000087"
     CDS             complement(36611..37270)
                     /locus_tag="U3989_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1713567.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37319..37822)
                     /gene="trmL"
                     /locus_tag="U3989_000088"
     CDS             complement(37319..37822)
                     /gene="trmL"
                     /locus_tag="U3989_000088"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN1713568.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38043..38909)
                     /locus_tag="U3989_000089"
     CDS             complement(38043..38909)
                     /locus_tag="U3989_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1713569.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39018..39659
                     /locus_tag="U3989_000090"
     CDS             39018..39659
                     /locus_tag="U3989_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN1713570.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39721..41082)
                     /gene="mgtE"
                     /locus_tag="U3989_000091"
     CDS             complement(39721..41082)
                     /gene="mgtE"
                     /locus_tag="U3989_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN1713571.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41100..41999)
                     /locus_tag="U3989_000092"
     CDS             complement(41100..41999)
                     /locus_tag="U3989_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1713572.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42001..42798)
                     /locus_tag="U3989_000093"
     CDS             complement(42001..42798)
                     /locus_tag="U3989_000093"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN1713573.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(42776..43480)
                     /locus_tag="U3989_000094"
     CDS             complement(42776..43480)
                     /locus_tag="U3989_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN1713574.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43611..44187
                     /locus_tag="U3989_000095"
                     /pseudo
     CDS             43611..44187
                     /locus_tag="U3989_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44305..44982
                     /locus_tag="U3989_000096"
     CDS             44305..44982
                     /locus_tag="U3989_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1713575.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45061..46869)
                     /gene="pepF"
                     /locus_tag="U3989_000097"
     CDS             complement(45061..46869)
                     /gene="pepF"
                     /locus_tag="U3989_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN1713576.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47045..48043)
                     /locus_tag="U3989_000098"
     CDS             complement(47045..48043)
                     /locus_tag="U3989_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN1713577.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48269..48922)
                     /locus_tag="U3989_000099"
     CDS             complement(48269..48922)
                     /locus_tag="U3989_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN1713578.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14718 a        10352 c         8685 g        15477 t
ORIGIN      
        1 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
       61 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
      121 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
      181 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
      241 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
      301 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
      361 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
      421 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
      481 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
      541 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
      601 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
      661 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
      721 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
      781 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
      841 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
      901 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
      961 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     1021 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     1081 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     1141 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     1201 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     1261 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     1321 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     1381 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     1441 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     1501 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     1561 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
     1621 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     1681 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     1741 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     1801 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     1861 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     1921 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     1981 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     2041 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     2101 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     2161 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     2221 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     2281 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     2341 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     2401 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     2461 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     2521 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     2581 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     2641 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     2701 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     2761 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     2821 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     2881 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     2941 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     3001 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     3061 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     3121 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     3181 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     3241 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     3301 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     3361 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     3421 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     3481 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     3541 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     3601 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     3661 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     3721 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     3781 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     3841 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     3901 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     3961 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     4021 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     4081 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     4141 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     4201 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     4261 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     4321 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     4381 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     4441 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     4501 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     4561 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     4621 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     4681 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     4741 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     4801 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     4861 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     4921 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     4981 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     5041 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     5101 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     5161 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     5221 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     5281 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     5341 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     5401 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     5461 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     5521 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     5581 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     5641 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     5701 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     5761 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     5821 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     5881 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     5941 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     6001 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     6061 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     6121 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     6181 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     6241 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     6301 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     6361 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     6421 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     6481 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     6541 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     6601 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     6661 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     6721 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     6781 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     6841 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     6901 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     6961 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     7021 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     7081 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     7141 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     7201 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     7261 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     7321 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     7381 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     7441 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     7501 gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat
     7561 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     7621 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     7681 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     7741 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     7801 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     7861 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     7921 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     7981 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     8041 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     8101 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     8161 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     8221 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     8281 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     8341 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     8401 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     8461 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     8521 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     8581 ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     8641 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     8701 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     8761 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
     8821 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
     8881 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
     8941 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
     9001 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
     9061 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
     9121 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
     9181 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
     9241 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
     9301 gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
     9361 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
     9421 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
     9481 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
     9541 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
     9601 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
     9661 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
     9721 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
     9781 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
     9841 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
     9901 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
     9961 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    10021 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    10081 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    10141 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    10201 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    10261 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    10321 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    10381 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    10441 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    10501 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    10561 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    10621 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    10681 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    10741 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    10801 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    10861 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    10921 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    10981 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    11041 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    11101 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    11161 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    11221 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    11281 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    11341 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    11401 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    11461 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    11521 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    11581 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    11641 aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    11701 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    11761 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    11821 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    11881 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    11941 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    12001 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    12061 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    12121 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    12181 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    12241 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    12301 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    12361 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    12421 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    12481 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    12541 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    12601 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    12661 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    12721 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    12781 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    12841 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    12901 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    12961 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    13021 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    13081 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    13141 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    13201 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    13261 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    13321 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    13381 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    13441 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    13501 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    13561 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    13621 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    13681 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    13741 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    13801 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    13861 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    13921 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    13981 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    14041 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    14101 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    14161 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    14221 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    14281 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    14341 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    14401 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    14461 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    14521 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    14581 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    14641 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    14701 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    14761 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    14821 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    14881 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    14941 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    15001 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    15061 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    15121 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    15181 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    15241 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    15301 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    15361 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    15421 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    15481 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    15541 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    15601 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    15661 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    15721 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    15781 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    15841 agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg
    15901 ttgcttgctg cttcacctag cccctgtact cgtgtttgat tggcgacttg accaaattga
    15961 ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc
    16021 aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca
    16081 gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa
    16141 atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa
    16201 atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct
    16261 tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat
    16321 gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta
    16381 agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca
    16441 acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta
    16501 cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt
    16561 tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa
    16621 ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat
    16681 tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct
    16741 aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct
    16801 acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt
    16861 gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc
    16921 ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact
    16981 attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa
    17041 atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg
    17101 ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga agggatgact
    17161 ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa
    17221 ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc tatttttgga
    17281 tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat
    17341 tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg
    17401 atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta
    17461 ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa
    17521 gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat
    17581 aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg
    17641 attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa
    17701 aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat
    17761 aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct
    17821 tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca
    17881 ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact
    17941 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    18001 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    18061 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    18121 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg
    18181 ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    18241 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    18301 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    18361 gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    18421 gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc
    18481 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga
    18541 atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    18601 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    18661 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    18721 gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta
    18781 tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta
    18841 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc
    18901 ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    18961 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg
    19021 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    19081 agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt
    19141 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    19201 tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta
    19261 atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca
    19321 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa
    19381 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac
    19441 ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt
    19501 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    19561 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    19621 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    19681 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    19741 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    19801 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    19861 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat
    19921 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    19981 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    20041 actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt
    20101 ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    20161 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    20221 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    20281 ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    20341 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    20401 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    20461 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    20521 taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt
    20581 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    20641 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    20701 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg
    20761 gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag
    20821 atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat
    20881 tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat
    20941 tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    21001 caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt
    21061 cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    21121 tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga
    21181 ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa
    21241 aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa
    21301 aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa
    21361 aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag
    21421 aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga
    21481 tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta
    21541 cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg
    21601 cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg
    21661 aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg
    21721 ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg
    21781 aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg
    21841 gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt
    21901 tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga
    21961 aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca
    22021 aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta
    22081 tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa
    22141 tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac
    22201 gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta
    22261 agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt
    22321 ttttattaat ccatcaattt cgtatatttt acttcatcga tcgtttttgt tcctgcaagt
    22381 tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc
    22441 ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt
    22501 gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca
    22561 cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct
    22621 agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt
    22681 actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg
    22741 attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt
    22801 ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt
    22861 ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa
    22921 tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca
    22981 ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca
    23041 tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca
    23101 gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct
    23161 gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg
    23221 gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta
    23281 ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct
    23341 tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt
    23401 tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat
    23461 aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta
    23521 ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact
    23581 atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa
    23641 caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc
    23701 atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg
    23761 ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag
    23821 agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg
    23881 aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc
    23941 tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa
    24001 aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa
    24061 tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc
    24121 atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg
    24181 caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat
    24241 agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt
    24301 agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc
    24361 tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc
    24421 catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt
    24481 attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca
    24541 tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta
    24601 atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat
    24661 tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg
    24721 gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc
    24781 ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg
    24841 ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa
    24901 caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg
    24961 cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag
    25021 attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt
    25081 tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc
    25141 cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata
    25201 gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact
    25261 tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg
    25321 tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt
    25381 ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa
    25441 cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc
    25501 agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact
    25561 atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca
    25621 attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc
    25681 gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt
    25741 tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat
    25801 atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa
    25861 aacgctacaa agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa
    25921 gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat
    25981 agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg
    26041 acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa
    26101 cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt
    26161 acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac
    26221 ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa
    26281 tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga
    26341 cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca
    26401 tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg
    26461 ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata
    26521 ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa
    26581 gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat
    26641 agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat
    26701 tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag
    26761 ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc
    26821 tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg
    26881 ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca
    26941 atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa
    27001 tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg
    27061 aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg
    27121 aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca
    27181 tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt
    27241 tgtgtttttc ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga
    27301 attaattttg taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa
    27361 agcttgctaa ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa
    27421 tagcaggagg aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc
    27481 ggaaatggct ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac
    27541 tatacacttc attatagtga atacaaagga cacgatgctg agattgctga aactttggca
    27601 aaagaaacat tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt
    27661 ttgatcatcg taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg
    27721 gaagtagaat tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg
    27781 gcaggccttc caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca
    27841 gaaaaagtcc acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt
    27901 aataatttcg gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca
    27961 aaaaagcgat tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt
    28021 gctttattca ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt
    28081 ttttcaaacg catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg
    28141 attgttccaa ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc
    28201 aatttcttta aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg
    28261 aaatatttcc atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat
    28321 ggtcaagaag atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact
    28381 aaacagttgt tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt
    28441 ttattcggaa aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt
    28501 taattctttg cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg
    28561 atcattgaaa acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct
    28621 gcaaatgttt gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa
    28681 tatgttggtg tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca
    28741 aacaccccac cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt
    28801 tctgagtttt ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac
    28861 aagaaagtat ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac
    28921 agaaccatta gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag
    28981 gttactttgc gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc
    29041 tctttgcgat taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac
    29101 ccataagaca agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat
    29161 cccgcactta gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct
    29221 gcatcgattg ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact
    29281 tgctgtgtaa caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc
    29341 atctttccta cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca
    29401 cgattaaaat aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact
    29461 gtttccttgt cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta
    29521 atcaaggcga ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa
    29581 aagaatgtgg caacaacagt gataattaca cctatcagta aattcgattt atttgcttta
    29641 agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt
    29701 tcctaacgca aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc
    29761 caaatcagtg tagaaataaa gtttatacaa actttcgctg atcaagttga cgagatttaa
    29821 ttgaccaaca atcggaatat gaagatcaat ccaatacttc acttgttccg accccatcat
    29881 accagcaaaa aagctcatcg caatcgtcac tgaaatcaag atgctatcct tttgcaccag
    29941 attcattttt ggtatcaaat taccaaccaa tgtacctaac aaaatcgcac acaacgaccc
    30001 cagagcggta acaatcatca accattgcca acgttcacca aagtctactt gatagataaa
    30061 acgaaagaca gctaacataa tataaatctc tgtcaaaaca atcgtaaagc ttgctgccaa
    30121 attagcgatt gaaactaacg attgctttct aggaatcaag gacaagcgaa taccatttga
    30181 cgattgattt gcttgctgat cctgtacatt gcggaccccc cacatcgtcc cgtagagaat
    30241 cgccattcca atcagagtga agaaataaaa acttttgaaa ctcatattac gacctgcatg
    30301 aacttcttga aaagatactt cttgttcaaa aacagtcatg atgggcgcga gatcaacgtt
    30361 ctccacttct gccactttac tcgaaattat accaatacgc tgtaaaaatt gatccatgac
    30421 gtttttcatc aacgtctgac tcattcctgc ttgacccaca tggagcgaaa cagatgaacc
    30481 atcaatgaga taaaaaccag ccaaatcatc tgcatttagc ttttcattcg cttctacttc
    30541 cgatagttcc tgtacttcaa ataatggttt atcattgact tcgacttgtt ctaagactgt
    30601 ggtaaaattt tccgaaagcg ctgtttgatc cgctttgatt acaattccag tattaatcgt
    30661 ttcgagattg ccaccttgat caatctcgcc aaaggccgcc ataaacatca atcctaaaag
    30721 aattggaaag gccaatgtcc aaaacaacat ggatttattt cgcagcagta tttttgtgcg
    30781 atatttataa aaatgccaaa acattatttc ccctcctcta gtctctcaaa tccttgccag
    30841 taatctctaa aaagacatca ttcaaggacg cttctttggt tgctattttt ccgtaactga
    30901 tcttttcttc ttgtaagatt gtaagaatcg gcaacaatac tgtatttagt tcagtagttg
    30961 ttacttggag ctgacctttt ttataagcga ttccatcaga agctattgtt cgctgacaac
    31021 acgttttctg ggagtaatgg gatgtcgatc gtcaaacgat ccgccgtccg taccatatct
    31081 tttagagatt ctttggttcc tttagcaatc acctgtcctt gatccatgat cacgatctgg
    31141 tcgcaaagaa tttctacttc ttccatgtaa tgtgttgtat agacaatggt tgctccttgt
    31201 cgattcagct ccttgatact ttctaaaatt ttattccgac tttgggggtc tacagctact
    31261 gtcggctcat ctaaaaaaat taaccgcggc ttgtgggcaa tcccacatgc gatgttcaac
    31321 cgacgtttca agccaccact aagttctcct ggacgaaacg aaacatattt ttctaatccc
    31381 actaattgaa tcacctcttc aatcaaggct ttccttttct ctttttcgga aacatataaa
    31441 ccgcaaaaat aatcgatatt ttcttgcaca cttagttcgt caaagaccgc aacgtcttga
    31501 ggaacgaccc caatcattgc cttgacagca taattttcag gagtcattgt tgtcccgaac
    31561 aatttaatgg ttcctttgtc ataatttaac aaagaaagca tacagttgat ggcagtcgat
    31621 ttcccactgc cgtttggtcc taacagacct aagacttctc cttcttgtac ttgcagatca
    31681 aaatgattca atgctacaaa atctccatag cgtttgacta atttatcaat ttcgataatc
    31741 atcatgaaaa actccttcca aatttcttgt tgatctgact ataataaagt gcaagaaaag
    31801 aaactagtga cgattggcta aaccacacat gacatttgtc acaatgaagg agtctgctat
    31861 gcataaatct cggctactca acgaaactag ctttctactg gttatctcat tgcttttgct
    31921 ggtaagtcaa ggattcgttg ctatgcaagt tatctattta ttgatcgccg ttttattcac
    31981 tggtgtcagt tatttactgc ctacaaagcc aaattggatt ttccaagtta ttctatttct
    32041 tttatgtgcc tttttacctg atcttgtcct gtttctgcca gcgacgatgc gagtgatcgc
    32101 ccctgaagaa agccaccttg attacttgtt tttgttaggt tttttgttga tttcgacggt
    32161 tggtaatcat ccccccatca aggtcgcctt tgtcgtaatt ttgctgtttt tttcctttta
    32221 cttacgaaaa aaagatcaac gaacgaccga tcttgagaaa aagtacattg tctccaaaga
    32281 tactcattgg gaaaaacaac aagagttatc ggaacaaaat aaagcattgg ccctgtcaca
    32341 agaaacacaa ttagccttgc aaattgtgga agaacggaac cgaattgccc gagacatcca
    32401 tgacaatgtc ggtcatctgc tgtcaagtgc gattttacaa attggtgcgc ttgaaatgat
    32461 caatcaagaa ccaaaactaa aagaaccatt agaaaattta agtgccaccg ttcacactgg
    32521 gatggatcgg attcgtcaaa gtgtccatga cctccatgag acatcgatct cttttcaaca
    32581 ctccttagag tttctgatcg ctgattttcc ttcttctgtt acgattgaag gaaacttgtt
    32641 tgaaggattg aatgaaacgc agcaaaactg cttcttaatg gtcatcaaag aagccttaac
    32701 caacagtatc aaacatagtc atgccaaaac cgttgtttta cattttcgaa cgttgccggc
    32761 cttttatcgc ttgcaaatca cgaatgatgg caccagccca caaaaaaatt ctgctcccgg
    32821 tattggtttg aaaacgatgc gtcaacgtgt tcaagaacaa aagggacaag tccatatttt
    32881 ccaagaaaac cagcaatttc agttaacgat cattttacca agggagggac accaatgata
    32941 acagtagtca ttattgagat gatccatttg tgacgaaatc gttacaaacc attttagaaa
    33001 gcacgaaaga gatccgtgtg ttaggcattg gtcattgtgc caaggatgcg ctagtcttat
    33061 acgaaaccca tcgtcccgat gttttgctga cggacatccg catgcctaaa caaacgggca
    33121 tcgatgctgc caaagaaatt ttggctaaat ttcccaaagc catccttttg cttttgacga
    33181 cattcaaaga tgaagaatac atccgcgaag cattttccat cggtgtcaaa gggtatttaa
    33241 tcaaacagaa cttgcaagcg attattcctt ctgtcaaatc cgcttataat ggacaggccg
    33301 tatttggtaa tgaaatcatc gagactttca ctcaaatgat gaagaataaa ccgacgattg
    33361 atagtcatgc ttatgcttct tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg
    33421 ccgggaaaaa caataaagaa attgcagatg ccttgtactt gagtgacggc actgttcgca
    33481 attacatcag ccaattacta gaaaagctag atttacggga tcgcacgcaa ttggctatct
    33541 atttttatca gcatttgcaa taagtcttat ctaacacaaa aaaacctgaa tgatgctcct
    33601 tcgcatcatt caggtttttt tcacatcttc tattcaattg tcccgcaaac catttcctca
    33661 cgattgaaag aatcaaggat tcgcagggct gccaggattc gttggattgt tcggcgtgtt
    33721 aggcggaaaa ataggcgcct tgtctaacgt tttttctagt tttgattctt cacgatcgtc
    33781 tcgttctaca tcaggacttt cattcaattt gaattctttt tccatctcat ctaacttttt
    33841 aaagtcattg ccagattctt ttgctaattt cgctaattta tcttcggttt ctttagccat
    33901 aaagtaacgc cccccttttg atttcagctt agcatatcac actgaaaaaa caaaagaaaa
    33961 cgctaaccaa cgatcatttt tagctatttc tcatctttaa aatcaagcat tttttgccgg
    34021 tataaaatcg tagggagaac tgttttccat accaatcatc ggatctacta actggttatc
    34081 gatcacttct gctgtaagaa cgaaatcttt agaaacagca ggttctacct cttctttctt
    34141 ttcttctcca acaaatgaag aaaacagatc tggttccact gtttccatta cttggttttt
    34201 cgtttcattc gtcccactgt catcttctac aggtagcttt tcttttacaa cccgtggctc
    34261 ttttgtatag ttcccatctt caaatggatc atttccgacc aaagaatctt cataagctgc
    34321 tagtttggtc cggagagtga tattcatttt atcattatcc atgatccttg atttcaacgt
    34381 agtcatccgg cttgctgatt cattcgctac acaagtgaca taggcttgct cttcccctaa
    34441 caaaatcaac agttccttac tgcgagtcac tgccgtgtag agcaaattac gctgaagcat
    34501 ccgctgatat tgatggacca tgggcaaaat aaccatcttg aattcactgc cttgtgattt
    34561 atggattgag caacaataag aaagagtgat tttattccat tcgtttcttt tataagtgac
    34621 ctcatttgca tcaaactgga taactaattc atccacttta tcttcagaat cttttgccaa
    34681 gatgataccc gtgatttctc ccatatctcc attgaataca ttcaattcgg gagtattgac
    34741 taattggagg actttgtctc ctatccgata aacggtgtcg ttccaatgta cttccttttt
    34801 tccaggtttc cctggattga atatctcctg catcatttta tttaacgcat cgatccctgc
    34861 aggtccccga tacattggtg ctaatacttg gatatcttgt gcacggaatc ccttattttt
    34921 cgctttttcc acaattttag agatcaaggg ttcaatttga taagcatcac ttcggaaata
    34981 ggaacgatct ttttgattct ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg
    35041 ggcaagagga ataatactag aaccatcacc ttgccgataa atctgattga gttccatttt
    35101 gggaatttgt tcgatttcca aaagatcatg gagtacttga ccaggaccga cagagggaag
    35161 ctgatctttg tctcctacta agataacttg catattcgtc ggaatcgctt tgaataatgt
    35221 gttagccagc catgtatcga ccatagacat ctcatcaaca atcaaaagtc cgccttctaa
    35281 ttctttcgta ctgactgaag gtgttttttc ccgtccattc agccccagaa gacgatggat
    35341 cgtactggca ggcagacctg tcgtttcgtt cattcgttta gctgctcggc cagttggtgc
    35401 tgcaagtaga ataggaaaaa tttcttgggt atattttgaa ggatccaatt ccagtccgtt
    35461 caactctgca aaaagattga cgattccgtt gatcacagta gttttccccg tacctggtcc
    35521 tcctgttaaa ataaatagag gggaacggat tgcttcagcg atagcttgtt cttgcgaagg
    35581 tccatactgt atgccgaata ttttttcgat acgccgaata ttcttctgca cttctttttc
    35641 aggataatgg atttcttttt tacgactgag aaggcgctgg atcgaattac cgatccccca
    35701 ttcagaaaag tacaaactgt tttcgtaaag attagtctct tcttgttgta tctttccttc
    35761 ttcaacaagt tggataatta cagtagcaac ctgatcaggc tggatttcaa ttgggcgact
    35821 tgtttctaaa atacggatcg tttgctgaag caatgtctcc gccggtacgt atgtatctcc
    35881 tgtttgaact gcatgctgaa aaatctgatg caagatagct gctcgaattc ttctcggtga
    35941 atctgctgca ataccgattt gttcggctag attatctgca cgtttgaacc cgattccttc
    36001 tatgtcttcg actagctggt acggattttc ttcgatgata tccaaagact cgttcttata
    36061 ggcctggtag atactgaaag ctaactgact accgaacccg taacgattca gtcccatgat
    36121 cacttgatcc atcccgtggt ttagccgaat cgtctcagca agcatttctc tttttggctt
    36181 cgtcaaaccg gagatttgtt ttaataccga aggatcagca atgatttttt cgatagcatc
    36241 ttctcccagc aattctacaa ttttttctgc tgttttcttc ccgattcctg ggaatttttc
    36301 gctggaaaga tacgctacga tcccatttgc agttgttggc ttttcttgct catatctttc
    36361 gagtttaagc tgccttccgt atttaggatg atccactaat tctccataaa aacgataagt
    36421 ctcattttct tgaatatcgc cgaaacttcc ggtgactaca atctcttttt cacggtaatc
    36481 agctgtggtt tcactgatgt gtaccaaaag cactttgtaa aaattactag gattttgaaa
    36541 aaaaatcgcg gcaactgtac caacgaaata aggcatttct tctggctgca tcggaagccg
    36601 cctccttctt ctatagtaaa ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt
    36661 tccatacttt cagttcatat ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca
    36721 aactactatt gaacaatcca cccatttcac gcaactcact tagctctttt cctgtcatcc
    36781 attgaatggc tgcagttaaa gaagctccat gtcctacgaa taaaagtggg ccatctttat
    36841 gttgggcaac cgcatctgct acaacagttt cgatacgtgt gattgccgat tcaatcgttt
    36901 ctccgtgaaa aggagaagga tcatacagat ctaaatgatt acgcaaattc ggcagattat
    36961 ccggatattt ctggtacatt tcatcgatcg actgaccttc taaagtaccc agccctagtt
    37021 ctcttagctt atctgtataa aaaatctcta ccggacgagc aagctctttg ttgatacctt
    37081 ctgctgtatc acgtgctcgc tttgctgtac tggaataaat tgctttaaaa ggaacatatt
    37141 ttaccttttt cccaaatgct ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc
    37201 cattgctccc ttgaaaacgt ctttcctgat tccattgtgt cttcccatgt cgggtaaaat
    37261 atagttccat acgcagcaaa ctcctgtctt tcttttattt tactctataa taaactgtct
    37321 aatccagctt gtcattttca tagtgatgcg tcaattctaa attagggaat ccttgctggc
    37381 gaagtgcttc atacactatc attgcagctg tattcgataa attcaatgag cggacatgct
    37441 catcattcat agggatgcgt aaacactttt cttcgttttc acgcataaat gtttctggca
    37501 aaccggttgt ttcttttcca aataaaaaga agtgctcttc tccatcagca aaattttcgt
    37561 cactatatac tttatgggca aattttgaga tcaaatgtag tttacgatta cctaaaaatg
    37621 ttaaaaaagc ttctaaatta ctgtggtaag taatatttac atcattccaa taatccaacc
    37681 ctgcccgttt tagatgtttg tcatcagttg aaaaacctag tggctcgatt aaatgaagcg
    37741 gagtattcgt ggctgcacac gtacgggcaa tatttccagt gtttgcaggg attaatggtt
    37801 caaataaaac gatatgattc atcaggatca ttcctctcat atttatgttc gtttctattt
    37861 tctgacaatt tgatttttgc ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag
    37921 tcgttacgtt agtgaagctc ttctcactaa cttttatcag aacaggaacc tgataaaaac
    37981 caatgaaaaa acaacttgtt gctaatataa caccctcaac ttttttttgc aacctctact
    38041 cattaaaaac tgctagaaaa acgttttctc ttagttcctt ttaataattc tatgtcaatt
    38101 gtaatcttct tcaaaggatt cttatataag tctgctttga ttcgctcttc tacctgccat
    38161 tctaaaggtg acatggccaa aagatctttt tgattttcct ctggtatctc aaattcatag
    38221 gttacacgct gacgttcagt taatttcatt gcttcagcaa acttggctac tacacgttca
    38281 ttggaataac tctgtttttc aggttgattc ggataaaaag cctgacgtag ttcctttaaa
    38341 taattttctt gtggcaccac cttcaacact tctccatctt ctgagagtac acgttggaat
    38401 tcttggtaat gagaaggcga aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac
    38461 ggcaaattgg tcaaatcagc tacacaccaa aatgcttcgg cagattgttc tgtagctgcg
    38521 tagactcctt cttttgaaat atcaaatcca acagcatttt gaatcttttt caattttgaa
    38581 agttcattca agaagctacc ttctccgcag ccaacatcta atagcgagac ttcttcaggt
    38641 aaatgctgcg caatagtctc aataacgggc tgatacatgc cagattggat cattctacgc
    38701 cttggaacga acattccttg atcgtattct gttttaaccg catgggataa aaaataaagt
    38761 gttccttttt tggaaaggtc aaaacggtga ttatttttgc atatcaagcc attatcagac
    38821 ttttttactt cttcttggca aaccggacag cggaacagct gatgattttc tgccaaaaac
    38881 tggcgtcctc ggtcaatttt ttttaacata gttccccctc atttctgttt tatcttaccg
    38941 ttagatgctc actttggcaa ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag
    39001 ataaaggaga agattagatg atcaaagaat tttgcgctga aaactacaca tctattcctc
    39061 tagcaattgc tagaggagcc gatcggatcg aattgtgtga caacttagcg gtcggtggca
    39121 caaccccgag tactggtgtg atcgaagaaa cgttaagtta tgctggggaa aaagatatcc
    39181 ccgtaatgac gattattcgt cctagagggg gcgacttcgt ctataacgat atcgaattga
    39241 aaatcatgca tactgatttg attgaagcaa agaaattagg gacggatggc gtagtatttg
    39301 gttgtcttac tccttcaggc tggctagacg aagaggcctt agaactattg atcgaaacag
    39361 cagaagggtt gcagatcacc ttccatatgg cttttgacag tattcctgaa gatcggcaat
    39421 tcgaagcaat cgattggctg gtagaacatg gtgttgaccg tatcttgact catggcggtc
    39481 ctgctgacaa aactattgta gaaaatttcc cacggcttaa agaattgatt gcctatacag
    39541 atggccgctt aatcattttg cctggtggtg gtgtcactgc agagaatgca gcacatgtag
    39601 ctaaagaatt gaacgtaagc gaagtacacg gaaccaaaat cgtggatctt cagttataat
    39661 cttataaaca aaaaaacatt tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt
    39721 ttacataaaa tagcccataa ataaagaagc aatattgaaa taaatcagta cactcgtcaa
    39781 atcgctcaaa gtcgtgataa agggaccgct tgctactgct ggatcaaatc ctagacgatc
    39841 cattagcatc gggattaaac tacctgcaag attcgccact gtgattgcaa atagcatagc
    39901 catccctatg acaaacccca agataaaatt atgtttccaa aaacctacga ttagaaaaat
    39961 cgtgatcccc gtaacagctc ctgtgacaac gccggtcaag acttcagcca aaacaagtcg
    40021 aacaaatcca tgatctttct catcagaaat cgcaagtcga cgaacagcca cagctagcga
    40081 ctgggttcca gcattaccgg cagttcctgt aatcagcgaa atgaagactg ctaaaatact
    40141 cgcttcactt accaagtctt catagtgact gattaaagaa gcggtcgaca tacctaaaaa
    40201 caacagagtg atcagccaag gcaaacgtcg agacgctgcc tttactggat tttcattaat
    40261 ttcttccaca ttgaccccgg caagtcctga ataatcgcta gcagcttcat catcaataac
    40321 gtcgatgata tcatcgactg tgacgattcc taacaaatga tcatcataat cggtaacagg
    40381 aagagctaag aagtcataat ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac
    40441 atgaactgaa agcactcgtt cgctcatcag atcagaaatc attgcgtcgt catcattcac
    40501 gattaggtcg cgcagagaaa taacccctac tagctgacct tcttgattaa cgacatagat
    40561 gtaatagatt gtttcagcta catctgcttc gttcttcagc acgtacatcg cagagcgaac
    40621 agtttgattg gctacgatcg aaacaaattc agttgtcatg atcgcgccag ccgtttcatc
    40681 ctcgtaatgg agaagttcct tgatttcact ggcatcgtct gccgacatca aacttaaata
    40741 tttagcaatt tgctttttat ccagcgtatt aagcaagtca acagcattat ctgtatacat
    40801 ctcagaaagc atatcagctg catagctagg acgcatttca tctaggtatt ctttcatatg
    40861 ttcgttgtct tcttcaatca catcgaacat atccgctaat tctttaggcg acagatagga
    40921 atagatcagc tgcctgttct cttctgtaag agattggtag aattgcccct gctcatagat
    40981 atgcatctct aaaaaactgt ttctgaatgc ctgaatatta ttttccctta aatcttgagt
    41041 caactgggca aatctttcct ctaattcttg attttcatcc aaaccaatct cacctcgcct
    41101 tattcttcaa accgtctcac aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc
    41161 agcaactctt ctgtaaatgg atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt
    41221 tttattccca gatccattcg cccgccatac atatcatcac cgagcagggc acagccaatt
    41281 gcggaaaaat gaactcttat ttgatgtgtc cttcctgtat gcagctggat atcaactaac
    41341 gcgatttctg ttagtcgctt ctctatccag tactcagtct gtgccgaacg tccttcatca
    41401 gagaccattc gcttcaatag cgaagaataa tctcgggcga tcggcaaatc gatatgtcca
    41461 tgtgccgata aatctgaaag ccttccactg actaaggcct gatatttttt cacaaacttc
    41521 ttttcacgaa gttgcatatc taattttgcg tgcgcaaatc catgctttgc aaaaagcatc
    41581 aaaccagatg tatcccgatc tagtcgcgtc accacatgga ttacttggtt ttcgtaacct
    41641 tgttttttat aataagcctt tacacgatta gccattgtcc catttggatg gtattgggca
    41701 ggaatagacg aaacgcctgc tggtttattt actaccagaa cgtgttcatc ttcatacacg
    41761 atatcgatcg gtgtttcatc caaaagtacg gtttcatgtt ctccttcagc aggaatcaca
    41821 atggttacct tatcatcctt agctagagaa aacaagacat tttctacttg atcattaact
    41881 ttgatttgac cgccttgaaa ttttattttc gctaacagac cctttgagat ccctttttct
    41941 tttaaaaaat attttacttg ctgagaatga tctttctcat aatcccatgt aaactgcata
    42001 tcaatcctca ctaataaagg catctttcac acgatgccaa aaatgcatat gtcgataaga
    42061 agcaaaatgg atccgttctt ctgctattcg ataataaatt gagcggatat ttgcttttgc
    42121 gacattcaat tgatcaaccg ttacaagata atcttctgta ttttccaatt ttatttctag
    42181 ccattcatca tgggcaatca cgatcggaga acccaaagta cgaaacactc gattattcag
    42241 tgaagcaatc tctgccaact gaaaggcatt gatactagga tggatcacag caccgccgac
    42301 acttttatta tatgctgtcg aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa
    42361 actttcaaac agctcgtttt tgatgtatac atcggctacc atcgtacgat tggcccgttt
    42421 aatcgttgat tcattcagtg ctaaaaaatg ctggtccggt gtttcatcca aataactgat
    42481 ccgaacatcc agcaagggat aactaacact ttgttcccgg ttcgttcgta agctgtctac
    42541 tagttcttcc agctcgtaat cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac
    42601 gcccaagaaa cgcacatcat tcagacaatg actgtagtga tggaacgccg acaataatgt
    42661 gccgtctccg ccgactgaga tgaccaattc tggctcatga tcatctcgtt gaatccctgc
    42721 ttgttctaaa agtgcgttca aacgttctgt cacttctata gtctttgctt ctttattatg
    42781 aacgatcgct actttcatcc cctgcctcct ctctttcgct ttctttcttc aaaagcgttc
    42841 gatagtagac atcatctgat tttcctcttc catgggagaa taaccgctgt gcttcttgga
    42901 tttcttcacg gatactggac atctcttcat ccaactgata ggcagcttct gccgcacgct
    42961 gaagtcgttc actcatttct tctggaaaaa cgccttgata tttataattc agcgaatgct
    43021 caatcgttgc ccagaaattc atcgcaagag tccgaatctg gatttccgcc aaaattttct
    43081 tctcaccgct gatcagctgt acaggatact cgatcaccag atgataagaa cggtagccac
    43141 ttgctttttt atttgtgatg taatctcgtt cttggagaac tttcatgtct tttcttttac
    43201 ggatgatctc aacaacttga tggatatctt ctacaaactg acacatgatc cttaacccgg
    43261 caatatcaga catttcttct tctaaacgtc ccattggaat atgccgtaat tgtgattttg
    43321 ttacaatact atcgactggc ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat
    43381 tttgttcacg gaactgtttg cggatccccc gcagtttcac tttcaattct gatacagttt
    43441 gttcatatgg tgctaaaaat gcttcccaat ctcgttccat agctgatacc tctacttcat
    43501 taacattcat tgtttattct accatatatg caaagttttt tcgtactctt gatttatgca
    43561 ttgaccaaaa aaagaaaatt aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa
    43621 tgcttgaaat cgaatataaa tctatgctga caaagcaaga gtacgagata ttcatcgctc
    43681 actatcaatt aacagataaa gatttccgtg tacagactaa tatttatttc gataccgttg
    43741 atgaacagct gaagaaacaa aattgtggcc tacggatccg tttcgttggt catcaagctg
    43801 aatatacatt aaaaacacct gctgaaaaag ggcgtctcga aacaacggat acctttataa
    43861 cagaagaagc agaagcattt atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc
    43921 aaaaactttc tgatttgaat atcgatcctg cttcattaat ccaaaccgga aagcttacaa
    43981 caaaacgggc tgaattccct attgaagaag gacttttagc tattgatgaa agctggggag
    44041 acaatctaca cgattttgaa ctggaactag aggtaagggg atgcatctgt gggaaaaatt
    44101 gcttttataa attttttaaa acgtttttct ttaccttatc gtcctgctaa gaacaagatc
    44161 caacgtatgc ttgaagctaa aaattagaaa cctttcattt tcacaaggca cttgagttca
    44221 tttttttact tttttctgtt aagttatagg atgtgaaaga atctagtcac tagttgtata
    44281 gaatctattt tgggggagat tcatatgatc gaaatctatt tatttgttaa tcctttaggg
    44341 gaatactgtt tcgaaatgga acaacagtta ttacaattta tcgaagaaga gtacgggaca
    44401 ccatcaaaag aaaagatgca atttcgcttt cttcctttgg tcaatttgca gacaattggg
    44461 gatgtcatgc aacaaaaagg aatttctcag aacaatcttg aggaacgtaa tcacttattc
    44521 tcaatgactt attctgcagc attggactgc aaagcggcgc agtttcaagg aaagaaaaaa
    44581 ggccgccgtt ttttgatcaa attgcaagaa gcagtcggct gtaatgacat accatattct
    44641 caagaacttg ctgaatccat atttgaagaa atcggcggcg atttgaatat gtttaaagaa
    44701 gatcgtcagt ctgacttcgt taaagaagta ttcttttctg atcaaaaaat tgctcgggaa
    44761 atgggcatca caaaacaccc ttctgcagtc gtttacaatt atgcctgcga ccgtgatttt
    44821 ggtgttttag tagaagtaaa aggtacaagt agcctttgga gaatcacaga gctgtgtaaa
    44881 acaacagata actcatatca agtttttaat ggtgaacatt tacatacaaa tgattctcag
    44941 ttccattcaa gaactagcca tttacacttg ctttctaatt aaaaaaatac gtcgactccc
    45001 ttacaagaga gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa atagaaagcc
    45061 ttataaacga tcgatcaatt cttctaattc atttagacgt tgttcaaaca tcgacattgc
    45121 atcttcaata tatgcagcct gtgtcatatc aactcctgct tttttcatta cttccacagg
    45181 ataatcgctg ttacctgctt ttaggtaagc caagtagttc tctagtgcct ctggttcctg
    45241 gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt attggaaaac
    45301 atagtaatta taataaaaat gcggtatccg tgaccattcg aatttaattt ctgggtcttc
    45361 ttcgactgct ggaccataat attttgcatt caatttacca taactatcac ttaggtattc
    45421 actagtcaat ggcacacctt tttcatcttc cgtatgcatg aaatgctcga attcagcgaa
    45481 ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag taatggttga gtacataagc
    45541 ccgtacacga gggtcttttt ctgtttccaa taaatactcc gttaggatat tttcattcgt
    45601 tgtcgaagca atttctgcta aaaagatgga gtagtcgccg tacacataag gctggttcga
    45661 acgagtgaaa taactatgaa cactatgtcc catttcgtgg acaagcgtaa atagctgatc
    45721 cagcgtatca tgccaattca ataaaatata tggatttgtg tcatagcttc ccgaagaata
    45781 agcaccgctt cgtttccctt tattttcgac aacatcgatc caacgttcag agaatgcttt
    45841 ttctacgatg gccatgtatt cttcacccat tggtttcagt gcttctaaag ctttttcttt
    45901 tgcttcttcg tacgtaaagg taattggagc ttcccctaag accggtgtat aaagatcata
    45961 catgtgcagt ttttccactt ctaataaacg tttccgtaat tccatgtatc gatgtaacaa
    46021 aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat acactttcag gaatatgatt
    46081 attgctcaaa gacgcttctc tggcagagct gtaattacgg actttcgctt taaaattatg
    46141 tccttttata tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt cgtaaacact
    46201 gtacaatccc ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta gcaactgacc
    46261 atacacgcca tgagataatt ggactatttc accattttct ccttcaatcg ttggaaaaac
    46321 tagatctgca ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga tttcacctgc
    46381 tccagcaaga agagattcct gctcagcaga taagacatgg gctcgattat ctactatttg
    46441 ctggatataa tggcgataga cttccaattt tggttcttct ttgaaatatt gccaaatctg
    46501 gtcatctgac aattgcaata tttctggttc aaaccaggaa acggcttcac taactttcga
    46561 aaacagactg cttgctcttg cataaagcgc ttggtaatct gtatttccag tgtcttgatc
    46621 gtttttaagg tgcgcatata cataaatgac ttcagtttgg cgataaaccc tcaatacgaa
    46681 ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt gtttttcaga
    46741 ttgttttaac tcttctgata attccaaata tttctcatca aactcttggt cgcttgaaaa
    46801 gatcttggtc aagtcccaag ttaaattttc aggcaattct tctcgttttg gcaactgctt
    46861 tacttccatg aaatccactc cttttcttcc cttagctcta tctatcaaaa tagatcatag
    46921 tctaacatcc acatgtcaca gcatctaact tttctactca ttcatgtgtc tgcttccatc
    46981 aattttagca taactaaatc ttgttttaca tagaaaaccg attcctagcc tatttttata
    47041 cttactattt tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc tgatagacca
    47101 tctcttcatc aatcatagga aaaagccatt cccttttatg ttcttttatt tggcaaagcc
    47161 atgagttgaa atcaggaact ttctggtttt ccaaggcttc ttcgtacaac attcgataaa
    47221 gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt ttgatagatc cacgatgata
    47281 gatgcaaaag atgtttatga cgcaagtagc attgttcttg tatggatact gtattctttt
    47341 gcttgtgaat aagttggcgt gtcagccatt tcttcccatc ttcactcact ttttctattt
    47401 ttttcatttg gttcatttta atttcgttac tagtgaatat ttctttaagg tccctactag
    47461 aaaaagtcca gcagctactg caaaaggaaa tatgccctga tacagtctct gtaatatgat
    47521 gatacaaata aagcttttgt ttcttgagat cagctttcca gcaatgcact cctctttttc
    47581 tattatagta gcagcaactt ttttctatca atgagaattg cgattgtcca agaaaagaat
    47641 taccaagtat ccaccatact tggtaactgt tattcaaata attttgggtt ctttcaataa
    47701 atctctggtg agacagtcta gaacattgga tttctattgc tagattcgcc aacaaaatat
    47761 ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa tactggttct actgactgtc
    47821 ccagccaatc catgaagcat tcctttaact gtaagtgctc ttttgattct ctttcactaa
    47881 agaccgaaca gttatctgat ttttgatggg caaaatgagc acattttttc tttccctttt
    47941 tcaaaatcac tctttttcga cagccacgac aataatattt ctttcctttt tctgcatgtt
    48001 tagcaaaaca gcacttatta tattcattca ttgcaagcag catttttctc cctctgaact
    48061 tatttacgtc taagaattta tttacacgaa aaaagagggc gtgaaaaagc tattctgcat
    48121 caagtaataa gaacagctaa acaaaaatag ttcccaatat ttttcgtttg gttgggcttg
    48181 ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg acacaatttt
    48241 tgtcacagtc tcttactata atgctgattt atacaaaata aaatttcgtt cgctcgatag
    48301 cattacgcgc catcagacat tgtccatatt cattcaatgt ttctggtgta cgatcgctct
    48361 tttcagcaaa ctccaaaatt cgagcaatct gattctctac atccgcttgg ttgagatttt
    48421 ccatccagaa gtgaacgact aggtaatagc gctcttcgaa taaatacaaa tcagtccaag
    48481 cagactctag ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct tcaaaagcaa
    48541 aagttgcttg tctttcctgt tcttctaggg cgcttccaga taattcttcc tccggatcgc
    48601 tgccaataat ctgctgacga agaaagtcac tcacatcttc tgaatcaaca gtagaatctt
    48661 ctgattgaga aatttcttca tccggaagat ttttactgat aaacagttct aatccgtctc
    48721 cttttggcaa aacttggaaa gtgacagctt cgcttcctcg gaactcctct tcgatatcaa
    48781 cttcttccaa aatactataa aagaaatttt cgatttcttt gtgattgcca agtaaatcca
    48841 aaaatgaaac gccccgttcc tctaaatctt catgaccaat tagtacacgg atcgtatttt
    48901 cattaatatg ttccatttcc attttgctac acctcacttt gccacaatgt ttaatataga
    48961 ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt aagcgaattt
    49021 aataaaacca gcggattttt caacctttca agctatctta tttaactata acatttttgg
    49081 cagacgaata ctatatagcc gacttttcag ctaattttcc ctctctattt ttcgtgcaat
    49141 catcatcttc tactaccttt ttatacatct aatcaagaat actttttgta aaaacaaaaa
    49201 aacttggaaa aactatcttt gtaatagtct tt
//
LOCUS       DASJON010000003        42301 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJON010000003 DASJON010000000
VERSION     DASJON010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.003"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..878
                     /locus_tag="U3989_000100"
     CDS             <1..878
                     /locus_tag="U3989_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1713579.1"
                     /translation="AQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFLEYSVL
                     EEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMTEIEVA
                     NQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYYEGYVS
                     DMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKHGYGEA
                     FGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDLLITED
                     GNRVLTRSPKELIIL"
     gene            1124..1549
                     /locus_tag="U3989_000101"
     CDS             1124..1549
                     /locus_tag="U3989_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1713580.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1542..1991
                     /gene="nusB"
                     /locus_tag="U3989_000102"
     CDS             1542..1991
                     /gene="nusB"
                     /locus_tag="U3989_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN1713581.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2088..2942
                     /locus_tag="U3989_000103"
     CDS             2088..2942
                     /locus_tag="U3989_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN1713582.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            2943..4280
                     /locus_tag="U3989_000104"
     CDS             2943..4280
                     /locus_tag="U3989_000104"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN1713583.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4285..4509
                     /locus_tag="U3989_000105"
     CDS             4285..4509
                     /locus_tag="U3989_000105"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN1713584.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4511..5401
                     /locus_tag="U3989_000106"
     CDS             4511..5401
                     /locus_tag="U3989_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1713585.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5410..6228
                     /locus_tag="U3989_000107"
     CDS             5410..6228
                     /locus_tag="U3989_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN1713586.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6335..6784
                     /locus_tag="U3989_000108"
     CDS             6335..6784
                     /locus_tag="U3989_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1713587.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            6802..8484
                     /gene="recN"
                     /locus_tag="U3989_000109"
     CDS             6802..8484
                     /gene="recN"
                     /locus_tag="U3989_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN1713588.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8539..8655)
                     /locus_tag="U3989_000110"
     CDS             complement(8539..8655)
                     /locus_tag="U3989_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN1713589.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(8728..9684)
                     /locus_tag="U3989_000111"
     CDS             complement(8728..9684)
                     /locus_tag="U3989_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1713590.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            9855..10223
                     /locus_tag="U3989_000112"
     CDS             9855..10223
                     /locus_tag="U3989_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN1713591.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10393..10824
                     /gene="mraZ"
                     /locus_tag="U3989_000113"
     CDS             10393..10824
                     /gene="mraZ"
                     /locus_tag="U3989_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN1713592.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            10845..11804
                     /gene="rsmH"
                     /locus_tag="U3989_000114"
     CDS             10845..11804
                     /gene="rsmH"
                     /locus_tag="U3989_000114"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN1713593.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            11809..12210
                     /gene="ftsL"
                     /locus_tag="U3989_000115"
     CDS             11809..12210
                     /gene="ftsL"
                     /locus_tag="U3989_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN1713594.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12211..14403
                     /locus_tag="U3989_000116"
     CDS             12211..14403
                     /locus_tag="U3989_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1713595.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14429..15391
                     /locus_tag="U3989_000117"
     CDS             14429..15391
                     /locus_tag="U3989_000117"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN1713596.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15405..16766
                     /locus_tag="U3989_000118"
     CDS             15405..16766
                     /locus_tag="U3989_000118"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN1713597.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            16792..17880
                     /gene="murG"
                     /locus_tag="U3989_000119"
     CDS             16792..17880
                     /gene="murG"
                     /locus_tag="U3989_000119"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN1713598.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            17895..19115
                     /locus_tag="U3989_000120"
     CDS             17895..19115
                     /locus_tag="U3989_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN1713599.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19271..20596
                     /gene="ftsA"
                     /locus_tag="U3989_000121"
     CDS             19271..20596
                     /gene="ftsA"
                     /locus_tag="U3989_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN1713600.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20619..21860
                     /gene="ftsZ"
                     /locus_tag="U3989_000122"
     CDS             20619..21860
                     /gene="ftsZ"
                     /locus_tag="U3989_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN1713601.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            21877..22554
                     /locus_tag="U3989_000123"
     CDS             21877..22554
                     /locus_tag="U3989_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN1713602.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22568..23167
                     /locus_tag="U3989_000124"
     CDS             22568..23167
                     /locus_tag="U3989_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN1713603.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23182..23469
                     /locus_tag="U3989_000125"
     CDS             23182..23469
                     /locus_tag="U3989_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN1713604.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23518..24300
                     /locus_tag="U3989_000126"
     CDS             23518..24300
                     /locus_tag="U3989_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN1713605.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24321..25028
                     /locus_tag="U3989_000127"
     CDS             24321..25028
                     /locus_tag="U3989_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN1713606.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25290..28088
                     /gene="ileS"
                     /locus_tag="U3989_000128"
     CDS             25290..28088
                     /gene="ileS"
                     /locus_tag="U3989_000128"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN1713607.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28137..29663)
                     /locus_tag="U3989_000129"
     CDS             complement(28137..29663)
                     /locus_tag="U3989_000129"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN1713608.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29678..30325)
                     /locus_tag="U3989_000130"
     CDS             complement(29678..30325)
                     /locus_tag="U3989_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN1713609.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30509..30838)
                     /locus_tag="U3989_000131"
     CDS             complement(30509..30838)
                     /locus_tag="U3989_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN1713610.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31015..31743
                     /gene="sfsA"
                     /locus_tag="U3989_000132"
     CDS             31015..31743
                     /gene="sfsA"
                     /locus_tag="U3989_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN1713611.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            31759..32772
                     /locus_tag="U3989_000133"
     CDS             31759..32772
                     /locus_tag="U3989_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1713612.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            32772..34049
                     /locus_tag="U3989_000134"
     CDS             32772..34049
                     /locus_tag="U3989_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1713613.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34112..36814
                     /locus_tag="U3989_000135"
     CDS             34112..36814
                     /locus_tag="U3989_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1713614.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            36966..37283
                     /locus_tag="U3989_000136"
     CDS             36966..37283
                     /locus_tag="U3989_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1713615.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37313..37633
                     /locus_tag="U3989_000137"
     CDS             37313..37633
                     /locus_tag="U3989_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1713616.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            37741..39201
                     /locus_tag="U3989_000138"
     CDS             37741..39201
                     /locus_tag="U3989_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1713617.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39269..39490
                     /locus_tag="U3989_000139"
     CDS             39269..39490
                     /locus_tag="U3989_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713618.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39521..39703
                     /locus_tag="U3989_000140"
     CDS             39521..39703
                     /locus_tag="U3989_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN1713619.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39703..40116
                     /locus_tag="U3989_000141"
     CDS             39703..40116
                     /locus_tag="U3989_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN1713620.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40239..41420
                     /locus_tag="U3989_000142"
     CDS             40239..41420
                     /locus_tag="U3989_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1713621.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41491..41655)
                     /locus_tag="U3989_000143"
     CDS             complement(41491..41655)
                     /locus_tag="U3989_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713622.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            41809..41882
                     /locus_tag="U3989_000144"
     tRNA            41809..41882
                     /locus_tag="U3989_000144"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41843..41845,aa:Arg,seq:cct)
     gene            complement(41951..>42301)
                     /locus_tag="U3989_000145"
     CDS             complement(41951..>42301)
                     /locus_tag="U3989_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1713623.1"
                     /translation="INQKELFENFEIKNPHNFVFFNLKNGLVSSNAVSKYLRKKLKEL
                     GIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKE
                     KINTVFDSLYKNDN"
BASE COUNT        14134 a         7183 c         9084 g        11900 t
ORIGIN      
        1 aggctcaagg gtttgaaatt attaaaaatg tcggacctat ttttgatgag gtagcaaact
       61 tagtacgcaa agaagaactg cactcattag cttttgagga aacaacagtc tcatttttag
      121 aatattcggt tttagaagaa atcatcgatg cggaattagt acctgtttca cagatgatcg
      181 aagaactcag agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata
      241 ttgcggatat ggcatatgat cacatcctga aaatgatcca accgggcatg actgagattg
      301 aagtcgcaaa tcaactagac ttttatatgc gttctcttgg cgcttcaggt gtttcttttg
      361 atacgatcgt tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa
      421 tcatcgaaca aggtgacttg atcacattgg actttggttg ctattacgaa gggtacgttt
      481 ccgatatgac acgtacattc gctattggtg atcctgggga aaaattaaaa gaaatttatc
      541 aaatagtctt agatgctcaa ttagcagtta ttgatgctgc aaaaccagga atgactggtg
      601 tccaattaga tgcagttgct agagattata tttcaaaaca tggttacggg gaagcatttg
      661 gacattctac aggacatggg atcggtttag agatccacga aggaccaaac gtttcttctc
      721 gtgcagaaaa gcaatttgta ccaggaaatg tgatcactga tgaaccaggg atctacttac
      781 caggaatcgg tggagtacgt attgaggatg acttgttgat cacagaagat ggaaatcgtg
      841 tgttgactcg ttcaccaaaa gaattgatca tcttatagtt tcatcaatag aagaagtaag
      901 cgtaaaaatc agtgttcaca gactttgtta tagaaaatca ctcgatttaa tgatacacta
      961 tagaagaact tttaagctgt aagagataat aaagttgggt gttttctcca attcatagca
     1021 gtttgcagct gatcggcggc agttttctta ccacgtgatc actatgccgc atcatagaga
     1081 attcggatgt atctcgtgct taggcaaagg gaggaataaa taaatggcag atgaaaaaaa
     1141 tttgatttta aacgcaaacc aagatttagg agagatcgtg atcgctcctg aagtcatcga
     1201 agtaattatc gggattgcag cttctaaagt agaaggcgta tacggaatgc gcggaacttt
     1261 tgccagcaat gtcactgaat tattaggacg tgcagctcac ggaaaaggtg tttacttagc
     1321 aaacgaggaa gatggcttga aagtagatct ttactgttat ctagaatatg gtgtttcagt
     1381 tcctaaagta gcaatggaaa tgcaggaacg ggtcagccag caagtccttt tcatgacgga
     1441 tatcgattta tctgaagtca atatccatgt agtagctgtt gttcctgaaa aattagaaca
     1501 accaacaatc gaagaacttt ttgaagacga agaggaagag aatgaataaa gaattatcaa
     1561 gacacgagat tcgggaaatg gcattgcagg ctttatttcc attagacttc aatgcagacc
     1621 taacaaaaga agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg
     1681 aagatgaatc agagtttgtt cctgtttatc ttgatacgct agttggcggt gtctgtgcaa
     1741 aaaaagatga attggataaa gtgattgaaa aacatttaaa aaacaattgg cacatcaatc
     1801 ggatctcaaa aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga
     1861 cagatgtacc agcggctgtt gctttaaatg aagcaatcga gttggcaaaa acatttagtg
     1921 atgaccgttc acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa gaattagaag
     1981 ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat aaatcgggaa
     2041 aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg gcagaattga
     2101 ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat
     2161 tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa aatccagcca
     2221 gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc cattcattgg
     2281 tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt gaaaaataca
     2341 atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag catatagatg
     2401 aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt catccgatga
     2461 atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca tacggcatca
     2521 tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta gttatcggac
     2581 gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat gcaactgtta
     2641 ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca gatattttag
     2701 tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca ggtgctgtag
     2761 tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat gtgaaatttg
     2821 atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta gggccaatga
     2881 ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga gcaggtgaat
     2941 agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa agaaaatttg
     3001 atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat ttccgtttac
     3061 gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata tctgctatta
     3121 tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg aaagtccttg
     3181 tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat gtagagcaca
     3241 tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa gaaaagctga
     3301 tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg
     3361 cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg gtgaaaagaa
     3421 gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac caagcagcgg
     3481 acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat acaatgatca
     3541 tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag cgggttgctc
     3601 gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa acagatacca
     3661 cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct gcagaattag
     3721 ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct agattgcagc
     3781 aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct
     3841 atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta gaccagttga
     3901 aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg
     3961 cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca gctcgccaag
     4021 aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg aaacaacagc
     4081 agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg aaaatcatgg
     4141 gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc accgagattc
     4201 atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga caagtacaag
     4261 agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc actgcaagaa
     4321 ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc agctctagat
     4381 tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc taaagcagaa
     4441 gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga cggaaacgag
     4501 gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg tagaaaaaga
     4561 aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg cgatgagtta
     4621 ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg ccgttgcttc
     4681 ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag aaatggtgca
     4741 tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc ttcgtagagg
     4801 aaaacctacc aatcataaag tattcggcga agcattagct atattggccg gagatggttt
     4861 gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc ctaagcttct
     4921 gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg ggcaagctgc
     4981 agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta ttcatgaaag
     5041 aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag cgaagcaacc
     5101 tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag cttttcagat
     5161 aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa cagctggtaa
     5221 agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa agacacgtga
     5281 ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag aagaggacgt
     5341 tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg tggagaaatg
     5401 agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg attatttgaa
     5461 acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga aaaaaacgag
     5521 cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt caaaggtaaa
     5581 aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa agcatttgat
     5641 ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg atttactgat
     5701 gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta caatcagcta
     5761 gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat
     5821 gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga tgtttctttt
     5881 atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga gggagaagtg
     5941 attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa aaacggaatt
     6001 gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa
     6061 ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga aggaaatatc
     6121 gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc agctattaat
     6181 atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat tgtaactgtg
     6241 aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata agctatcatt
     6301 gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt
     6361 gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg ttgaaatcct
     6421 gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa aggaactgaa
     6481 actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag ctgaaacaaa
     6541 cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg cagttgacca
     6601 aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag cagcaaattt
     6661 gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg ataatgtttt
     6721 gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc tgcattacct
     6781 ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt tgcgattatt
     6841 ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga gactggtgcg
     6901 ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg ttcaagcgat
     6961 tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa
     7021 gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga caatctgatt
     7081 gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg tcatattatc
     7141 acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg acaaaacgaa
     7201 catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt tggagatgta
     7261 gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg tgagctggaa
     7321 aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat agatatgctc
     7381 catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga agagaaatta
     7441 aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt agctgcaggt
     7501 tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt ggccgtttca
     7561 gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga aaatatccaa
     7621 agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat tgatttattg
     7681 gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat ccgacagttg
     7741 aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga aatcaccgaa
     7801 gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga ggaactagag
     7861 caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc
     7921 gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat ggaaaacact
     7981 gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga tggatttgac
     8041 caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt ggtgaaagtc
     8101 gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt ttcttctgag
     8161 caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg aagagttgca
     8221 caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt gtgcatcact
     8281 catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa agcagtgatt
     8341 gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt
     8401 gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc aaaagaacta
     8461 ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct
     8521 gatagctata ttttattttt acatgacact cattacagca gtcaatacaa cagatccaat
     8581 cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag tacttgtttg
     8641 ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag tgtacaatct
     8701 tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta aataataggt
     8761 agttacgata caaataaata atgttaatag aactagccat ataaaggcat catccctttc
     8821 agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg gtatagttaa
     8881 aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg atacgattgc
     8941 tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag tagtcattgc
     9001 ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat tttcttggaa
     9061 aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg cttctctgaa
     9121 ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt cagtggatac
     9181 ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat aactgttgta
     9241 agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg aaatatcact
     9301 ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat tcgtaattgt
     9361 gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca cttgaaaata
     9421 accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg gctttgtcaa
     9481 ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata tggaagtgat
     9541 gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt gattaggacg
     9601 ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca gtttctcatt
     9661 atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt ttattataac
     9721 ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa ctgcataaat
     9781 ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca acgataaaaa
     9841 aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat atttacccgg
     9901 taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat cgtcgtttta
     9961 aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat gcattgtcta
    10021 gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg ctaatgggaa
    10081 tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg cgttatttca
    10141 agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc ttgatcattg
    10201 tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc
    10261 tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc
    10321 ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta gtggaggaat
    10381 ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta
    10441 atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac acgcggattg
    10501 gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc taaactgaac
    10561 gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta ttcggctgcc
    10621 actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt aagaaattat
    10681 gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga aatttgggat
    10741 gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga aattgctgaa
    10801 agtatgattg atttcggact ataagaatag gaagaggctg actaatgact gaaacatttc
    10861 aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa ccggatggcg
    10921 tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta agccaactaa
    10981 gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc
    11041 tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg aacttcagag
    11101 aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg tatgatctag
    11161 gggtttcctc tcctcagcta gatgaagcag aacgtggatt tagttatcat caagatgcac
    11221 cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta attaatgaat
    11281 attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa ttttccaaac
    11341 aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca actggtgagc
    11401 ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg
    11461 caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt gccgaagagg
    11521 cctctttaga acaggcaatc cggttattga aagtcaatgg aagaatcagc gtaatcacat
    11581 tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt acggtacaag
    11641 atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg aaagtcatta
    11701 ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga tcacgcagtg
    11761 caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat ggcagaatta
    11821 aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc ggaacaacaa
    11881 atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc aaaacgatta
    11941 aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc tattgttgct
    12001 ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg
    12061 tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa
    12121 aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg attgtctatt
    12181 aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat tcggcggttt
    12241 atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat cattttattt
    12301 gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat cgttattgga
    12361 ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca aggaagtgaa
    12421 gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct cgcagaagat
    12481 gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg ggataaaaaa
    12541 ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa gaatctttct
    12601 atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga gctgtatcaa
    12661 gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg
    12721 gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc tcggatgtat
    12781 ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa agatgagaat
    12841 ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag cggtaaagat
    12901 ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac agtggcagaa
    12961 gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag actacaaagt
    13021 tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga attgacagca
    13081 gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc tactttcaat
    13141 cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt cttggtacaa
    13201 gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa
    13261 ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga agatgctacg
    13321 atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg
    13381 tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg gtataactat
    13441 ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga agtaaacggt
    13501 gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca agcggtaggt
    13561 gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg tacaatgatt
    13621 caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg aacaactcaa
    13681 acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg
    13741 agagatgttg tcgaaagcga aaactacggt agtgcttatg gtgtatatag tgtacctgga
    13801 tacaatgtat cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg gggatatcaa
    13861 acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga agatccagac
    13921 tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc attggctaaa
    13981 atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga agattcagat
    14041 acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga agcggacgtt
    14101 gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa tggtaaaaaa
    14161 gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa actgatccta
    14221 tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc agatatcatg
    14281 aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta ttgtgtcaaa
    14341 caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac tttagaagaa
    14401 taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga ttcccattgt
    14461 cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt ttcaaatgaa
    14521 aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca aagccggaac
    14581 tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa tttgggttgg
    14641 agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat tagcgttata
    14701 cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa acatgggact
    14761 gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt atcttgttta
    14821 ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac ctttaggaat
    14881 catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag taaatctgac
    14941 agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag cttacggtct
    15001 aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg ttttaggagg
    15061 tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg gagatgtcgg
    15121 ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc aagaatggac
    15181 attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc ttcaagttac
    15241 ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc atcactttga
    15301 aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca gcattgtgac
    15361 ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa
    15421 catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta agtgcggcca
    15481 aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct tttgacgaaa
    15541 atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt agtcatccaa
    15601 tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt ccctacagtc
    15661 atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa gttgagctag
    15721 cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga aaaacaacga
    15781 caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt gcacgtttag
    15841 cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa
    15901 ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc cctcatatag
    15961 cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga aaagaatatg
    16021 tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg atccttaact
    16081 ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta ctgcctttct
    16141 cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata tactataaaa
    16201 aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat gtagaaaatg
    16261 cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct atacgagaaa
    16321 cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa atccaaggca
    16381 gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg gctctaagtg
    16441 gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga aatacatttg
    16501 atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc caaacaaaag
    16561 aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca gccgattctg
    16621 tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta gttttattat
    16681 caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga aacagattca
    16741 tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt
    16801 ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt tgtcaattat
    16861 gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa
    16921 aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca aggatttaaa
    16981 cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag tatccgtcaa
    17041 gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg aggatatgtt
    17101 tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat ccatgaacaa
    17161 aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg gatcgctctt
    17221 tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt aggtaatcct
    17281 cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt tgatttggat
    17341 ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa aatcaatcaa
    17401 gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt gtatgcttca
    17461 ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca aaatattagt
    17521 gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct gctggtgggg
    17581 cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc agtgttggtt
    17641 cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct tgttcacgca
    17701 ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag tcagacagtc
    17761 aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc taaagaacaa
    17821 ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat caaaaaataa
    17881 aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg
    17941 atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca gaacaagaga
    18001 tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt tcagattctg
    18061 aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg
    18121 aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt tcttttttga
    18181 accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg ttagctttga
    18241 tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct ccttatagca
    18301 aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa atcatctctg
    18361 atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga tctacttata
    18421 tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat ttcaaaggga
    18481 tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc gcaaaaaaca
    18541 atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta gcaaatccta
    18601 caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa gaattaacaa
    18661 agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc aagtacacgc
    18721 caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat caagttattg
    18781 taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc aaagatatgg
    18841 atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac ggtagcactc
    18901 aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa aatgcggatc
    18961 aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta
    19021 gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata gaagataatc
    19081 caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa aagtaagaat
    19141 gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat atctattaat
    19201 aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta agtaggaggg
    19261 aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt
    19321 taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc
    19381 aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat
    19441 acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt
    19501 cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg
    19561 agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg
    19621 ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg
    19681 gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct
    19741 gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg
    19801 attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga
    19861 cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc
    19921 tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt
    19981 cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat
    20041 aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt
    20101 gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc
    20161 tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga
    20221 gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt
    20281 ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag
    20341 aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt
    20401 gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt
    20461 tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga
    20521 tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac
    20581 aaatattttt gattaattca tcctgggagg aataagatat ggaattctca attgataata
    20641 atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg
    20701 taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc aacacagacg
    20761 tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc aaatatactc
    20821 gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa gaaagtgaac
    20881 aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca ggaatgggtg
    20941 gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat
    21001 tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt ggccgttttg
    21061 ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt atctcaaata
    21121 accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc cgtgaagcag
    21181 ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg ccaggttacg
    21241 ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg
    21301 ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct
    21361 ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac atcacaggcg
    21421 gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca aatgcagcaa
    21481 ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc gatgaaatcc
    21541 gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac
    21601 cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat atggatcaag
    21661 ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc agacgtgaac
    21721 aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa aaagaatttg
    21781 atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat acgccaccgt
    21841 ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa cttgcagaaa
    21901 atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca
    21961 ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt tagagctggc
    22021 gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca agatttattt
    22081 gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg
    22141 attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc agaagaaatc
    22201 cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc tggtgaggaa
    22261 tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt agctgcctgc
    22321 acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc tgatgagcaa
    22381 atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat
    22441 gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca aattgcgatt
    22501 gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga ataaaggagg
    22561 aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat tagctgatga
    22621 ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac agccaactaa
    22681 aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata ctcatgaaaa
    22741 acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa
    22801 aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa gaacagcttc
    22861 aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa tggaaccaag
    22921 agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag caaccttagt
    22981 gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc taacaggaac
    23041 agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc tttgtacacc
    23101 gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga atttgtttga
    23161 tctgtaagag gaggaactac gatttatact attgcactat tacttcattt acttcaacgg
    23221 gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg gtttccaggc
    23281 gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta tatcagtttg
    23341 ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg ggcaatcctg
    23401 atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac tgtccattta
    23461 gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg gaaaattttg
    23521 gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga ttcggtaggc
    23581 gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt tcttgaccca
    23641 agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg tttcatgttt
    23701 tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga ttattacgaa
    23761 cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag caaatttgcc
    23821 agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa acgctcttac
    23881 tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat
    23941 cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc
    24001 aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg aacagttagt
    24061 tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca gcggtccaag
    24121 cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag acctgatttt
    24181 gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca gatccaagaa
    24241 cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt acgtaaataa
    24301 aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat aagacattcc
    24361 cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat ttagttgttc
    24421 gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc aaacattctg
    24481 aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca atcatcgtag
    24541 ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta
    24601 cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat
    24661 taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa
    24721 gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc catcaacgta
    24781 tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg gatgaaattt
    24841 tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa gttttagcaa
    24901 aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc atagaagcac
    24961 ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt gaaaatgaaa
    25021 acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga gtggaagttc
    25081 ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg aaattagagt
    25141 aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat agatgcaact
    25201 aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc
    25261 ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg caattaggaa
    25321 aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat
    25381 gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa cctagctttg
    25441 ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat tcattgaata
    25501 aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt gcaccatatg
    25561 ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat aaaggcgtga
    25621 aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat gcactttccc
    25681 aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat tgggaacatc
    25741 cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg
    25801 ctgaaaaagg ctatatttac aaaggattga aaccaattta ctggtctccg tcaagtgagt
    25861 cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct atttttgtag
    25921 ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt gtcatttgga
    25981 cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt
    26041 atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag
    26101 tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa
    26161 tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg attttaggcg
    26221 atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga catggggaag
    26281 atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta gactcaaaag
    26341 gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa gcaaatccta
    26401 tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc tttactcata
    26461 gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt
    26521 ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa gtagagtggg
    26581 ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc gattgggtaa
    26641 tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa aatggagaag
    26701 caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa catggatcaa
    26761 ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc cacccaggtt
    26821 ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt gattctggtt
    26881 cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca gatatgtatt
    26941 tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact agtgtggcta
    27001 tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta gatggtgaag
    27061 gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat
    27121 ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg
    27181 tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga aatacgatgc
    27241 gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt tcgtacaatg
    27301 atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc
    27361 gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt atgaacttct
    27421 tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt tatattgaag
    27481 cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt
    27541 tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg tcattcctga
    27601 aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag tttgcaaacc
    27661 aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat gtcttaaaag
    27721 cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca aaactaactg
    27781 tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat attgcacaat
    27841 tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa gtgccagata
    27901 acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg gaaacttgcg
    27961 atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct catctatgcg
    28021 gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa ggattcgaag
    28081 aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt ttgttatcaa
    28141 tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca ttcgaatccg
    28201 tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt gtagttggga
    28261 aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg ccaagattga
    28321 gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc taatagtaat
    28381 ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa ttccaatttg
    28441 attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa agaaaatcct
    28501 tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt
    28561 tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat gttgatccga
    28621 gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc tgaccaacgg
    28681 aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg agcatccact
    28741 ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg agcatattgc
    28801 ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc tttcaatgct
    28861 ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc cattgcaagt
    28921 aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc actatgatcg
    28981 tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg gatattgtcg
    29041 atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat agctgagatg
    29101 ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg ttctgggaat
    29161 acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc gaacggtttt
    29221 tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt caaatgagaa
    29281 acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt accgtttaat
    29341 tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg cgttgaatca
    29401 tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa
    29461 ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc gctccaggga
    29521 cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg
    29581 aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa tatcgtaaac
    29641 aatacatttt tttcattaga catgtggata cactccttta tattttatcg acaaaaatcg
    29701 tgctggccgc cttaaagctg acggggattt tctttctatc attttggata gtgatcggtc
    29761 cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg tcaatagtga
    29821 ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact tttgtaccga
    29881 caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct tttttaggaa
    29941 tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa tgatctgcaa
    30001 gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca ttccagctat
    30061 agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc agactgctag
    30121 ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt tctacaagct
    30181 cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca tctagactag
    30241 atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa atgattttca
    30301 aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa aaccatcatt
    30361 agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa caaaggattt
    30421 ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct ttcgtttata
    30481 aaggatatat atatacattg ggagggatct atttttcaga taacaactta tatgaagtgt
    30541 ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa aaaccatact
    30601 gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca agagactgac
    30661 cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc tggctatcac
    30721 taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct tgttttttct
    30781 ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa tagatcattt
    30841 tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat tctttcttct
    30901 ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact ggttttttta
    30961 ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca taatttgaag
    31021 tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat agcacattgt
    31081 atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag aggaaaagag
    31141 gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa acggaaaacc
    31201 gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga tagtcaattg
    31261 cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc aggattaact
    31321 ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt tgatatttat
    31381 ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac acttgaaaat
    31441 aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca tataggagaa
    31501 ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc tcagttcgaa
    31561 catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga tatggttcga
    31621 catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc ggcagatgag
    31681 gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga tcctatacaa
    31741 tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg gattactcat
    31801 caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt atattctaga
    31861 catttggaaa aagcacagga atttggttct cattatgaag gggatatcga attcgcaaca
    31921 gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc ttctccgaat
    31981 agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt gatagttgaa
    32041 aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc aaatgaacac
    32101 aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa aaagattgcc
    32161 gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat gaaatattct
    32221 tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac tcatttctca
    32281 ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc ttggtttgga
    32341 atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga tggtcttgga
    32401 tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa aatcggtgat
    32461 tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa tggagtcaat
    32521 gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga tttggcggta
    32581 acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt catggctcat
    32641 ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc tagaaatgtg
    32701 aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc agataatgag
    32761 gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag aaaaaatatg
    32821 aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag ccactaagta
    32881 ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg gcttatcttt
    32941 ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt
    33001 ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa gacaaagagt
    33061 tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag ggtttgaagg
    33121 aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa
    33181 aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag ctatttgacg
    33241 ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag tatcagctag
    33301 cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa
    33361 cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg agacattact
    33421 ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg catatagaag
    33481 attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg gaagaaaaat
    33541 tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag ctactccgaa
    33601 aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc acagatgtgg
    33661 cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa gttccgttaa
    33721 gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat gaaggaatag
    33781 taattacact cgtacaagat aataatctaa gagatttaaa acggctttta cgtccatcaa
    33841 atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag cagccaacca
    33901 aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct ggaaaggagc
    33961 agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag
    34021 agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt atgaaaacgg
    34081 gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca
    34141 gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc gcttaactat
    34201 cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc atattgtaaa
    34261 aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat ctgatgaagt
    34321 gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa atgattcttt
    34381 aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg ggttgcatac
    34441 gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta tgtatcattt
    34501 tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca attgtgcgga
    34561 ttatgatcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa aaggggctta
    34621 cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg gtatattgtt
    34681 tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta ctttcattga
    34741 taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat ctgttcagat
    34801 cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc gacggattcc
    34861 tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt atgaaatcat
    34921 ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga
    34981 agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga
    35041 cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg agaaaaagct
    35101 aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga aaatcaagga
    35161 aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga catttgaacc
    35221 aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg tctataacga
    35281 tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg atctagagac
    35341 cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat tgactaaaag
    35401 ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc aattgtatga
    35461 tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg cttttgcttt
    35521 gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc acccagattt
    35581 gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc tttcaacgat
    35641 cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta ttagtatgtt
    35701 tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag atgttctcat
    35761 attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag aactattagg
    35821 aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa caatgtatga
    35881 agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc tactcttgtt
    35941 aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga ctggcattcg
    36001 gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac ggatggcaag
    36061 tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg aatcgatcgt
    36121 acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt
    36181 gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta tgccagtgat
    36241 taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat cattcaaaaa
    36301 acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa cagttgatgt
    36361 tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa
    36421 tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg attcacttgc
    36481 tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc aaaagacagt
    36541 tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata ctggaatcgt
    36601 tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc ggcaatttga
    36661 gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc gggcaaactt
    36721 gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg catttcttac
    36781 acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact gtttacacac
    36841 taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct
    36901 ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt
    36961 taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt acgagtttat
    37021 tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat atttttgcgg
    37081 tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta ttattattag
    37141 gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc
    37201 cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa gttcttgcac
    37261 aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca acgtggaaga
    37321 tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg caaagagcga
    37381 tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg ataggaaaat
    37441 cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca tgttgacaca
    37501 agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc aagatcactt
    37561 aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt
    37621 atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt tgaacggatt
    37681 ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag gaagtaaaaa
    37741 atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc aaaaatcgga
    37801 gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcatactgcc cgcaacaatg
    37861 gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca acagtttaac
    37921 agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt gaatgtcatc
    37981 atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt gatctttgcc
    38041 ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc tggggggatc
    38101 gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc agcagcatta
    38161 gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat tggtgcaacg
    38221 attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga tcatttgaat
    38281 ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat tttttgtaaa
    38341 ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc agtatctaaa
    38401 gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat tatcaattat
    38461 atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa atatattgca
    38521 gaaccattcc taggattatc tcaagggttg ggagcagtat tgatcgtcac agtatttgtt
    38581 caagtattct ggttctttgg tattcatgga tcaaatgtat tggcaccagt gctagaggga
    38641 atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta taacggaaaa
    38701 actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat gtgggtccgc
    38761 ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt cttactgata
    38821 gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt atccattggg
    38881 cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt attgaatgca
    38941 atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg atggacagct
    39001 acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt accgccgttc
    39061 ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac attagtatgt
    39121 atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa aatggaccct
    39181 gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag tttcgttcct
    39241 cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa tgcaacaaga
    39301 tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta catttaaaga
    39361 aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag ttgcggctaa
    39421 aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt ttcaatcaac
    39481 agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa acggtttatt
    39541 tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta
    39601 tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct atttcttcat
    39661 gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat cacgaaaaaa
    39721 ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct tttcgatgta
    39781 cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga gtacgtcaaa
    39841 caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga aaaccagtct
    39901 tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca aatcaaacca
    39961 ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta tggcatgctt
    40021 caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg gagacgtttc
    40081 aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg gtactattta
    40141 gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga tctttcctgc
    40201 gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc
    40261 taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt aatggggcag
    40321 atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg aattacggat
    40381 tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt ccagtgaatt
    40441 tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat ccagaagcaa
    40501 ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc cgtgatcgta
    40561 aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc tatggctatg
    40621 aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg tttatcaaaa
    40681 aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca atcagaagaa
    40741 tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac tggtggtata
    40801 cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat gataatgagg
    40861 aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt gcggagtggg
    40921 gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg
    40981 cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat tatctattag
    41041 aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc gtagatattt
    41101 ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc ctacaaatag
    41161 aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa
    41221 aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga agtatccaac
    41281 atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc caagaaagaa
    41341 tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag caacagccaa
    41401 ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc accacccttt
    41461 tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt
    41521 gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga ttgcttcttt
    41581 cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata ctttttcttt
    41641 tggcttatca ttcattggaa aggacctccc tacattattt cattctacct ctagtttatc
    41701 atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt tttagcagat
    41761 aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt tcctgtagct
    41821 cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa
    41881 cgtataaata acccctgtat cctttgcggt acaggggttt gttttgtatt tgtgccaaaa
    41941 atgtgccaaa ctagttatca tttttatata aactatcgaa cacagtattg attttttctt
    42001 tatcctgatc ttcaagctct cgaacaatat gaagataagt agacattgta gtttctaaac
    42061 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
    42121 atgcatgtgt atgccttagt ccatgcaaag taaattgctt ttcaatccct aattctttta
    42181 attttttgcg caaatatttg ctgacggcgt ttgatgaaac taatccattt tttaagttga
    42241 aaaaaacaaa attatgaggg tttttgattt caaaattctc gaataattct ttttgattga
    42301 t
//
LOCUS       DASJON010000004        41081 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJON010000004 DASJON010000000
VERSION     DASJON010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.004"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..1125
                     /locus_tag="U3989_000146"
     CDS             79..1125
                     /locus_tag="U3989_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1713624.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="U3989_000147"
     CDS             1474..2106
                     /locus_tag="U3989_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN1713625.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="U3989_000148"
     CDS             2694..4133
                     /locus_tag="U3989_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1713626.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="U3989_000149"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="U3989_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="U3989_000150"
     CDS             4895..5110
                     /locus_tag="U3989_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713627.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="U3989_000151"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="U3989_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="U3989_000152"
     CDS             complement(5770..6681)
                     /locus_tag="U3989_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1713628.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="U3989_000153"
     CDS             complement(6700..7704)
                     /locus_tag="U3989_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1713629.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="U3989_000154"
     CDS             complement(7701..9824)
                     /locus_tag="U3989_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1713630.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="U3989_000155"
     CDS             complement(9829..12276)
                     /locus_tag="U3989_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1713631.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="U3989_000156"
     CDS             complement(12263..12652)
                     /locus_tag="U3989_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1713632.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="U3989_000157"
     CDS             complement(12712..13215)
                     /locus_tag="U3989_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1713633.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="U3989_000158"
     CDS             complement(13228..13452)
                     /locus_tag="U3989_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713634.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="U3989_000159"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="U3989_000159"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1713635.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16349)
                     /locus_tag="U3989_000160"
     CDS             complement(16212..16349)
                     /locus_tag="U3989_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1713636.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16346..17530)
                     /locus_tag="U3989_000161"
     CDS             complement(16346..17530)
                     /locus_tag="U3989_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1713637.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="U3989_000162"
     CDS             complement(17789..18079)
                     /locus_tag="U3989_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713638.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="U3989_000163"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="U3989_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19287..19451)
                     /locus_tag="U3989_000164"
     CDS             complement(19287..19451)
                     /locus_tag="U3989_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713639.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19444..20118)
                     /locus_tag="U3989_000165"
     CDS             complement(19444..20118)
                     /locus_tag="U3989_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1713640.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20196..21536)
                     /locus_tag="U3989_000166"
     CDS             complement(20196..21536)
                     /locus_tag="U3989_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1713641.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21619..21954)
                     /locus_tag="U3989_000167"
     CDS             complement(21619..21954)
                     /locus_tag="U3989_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN1713642.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(21964..22338)
                     /locus_tag="U3989_000168"
     CDS             complement(21964..22338)
                     /locus_tag="U3989_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1713643.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22351..22665)
                     /locus_tag="U3989_000169"
     CDS             complement(22351..22665)
                     /locus_tag="U3989_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1713644.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="U3989_000170"
     CDS             complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="U3989_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1713645.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            26793..27116
                     /locus_tag="U3989_000171"
     CDS             26793..27116
                     /locus_tag="U3989_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713646.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            27280..28197
                     /locus_tag="U3989_000172"
     CDS             27280..28197
                     /locus_tag="U3989_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1713647.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28190..28972
                     /locus_tag="U3989_000173"
     CDS             28190..28972
                     /locus_tag="U3989_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713648.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            28973..29653
                     /locus_tag="U3989_000174"
     CDS             28973..29653
                     /locus_tag="U3989_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1713649.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            29646..30542
                     /locus_tag="U3989_000175"
     CDS             29646..30542
                     /locus_tag="U3989_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1713650.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            30754..32676
                     /locus_tag="U3989_000176"
     CDS             30754..32676
                     /locus_tag="U3989_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEN1713651.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            32677..33123
                     /locus_tag="U3989_000177"
     CDS             32677..33123
                     /locus_tag="U3989_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1713652.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            33139..33453
                     /locus_tag="U3989_000178"
     CDS             33139..33453
                     /locus_tag="U3989_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN1713653.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            33479..34528
                     /locus_tag="U3989_000179"
     CDS             33479..34528
                     /locus_tag="U3989_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN1713654.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            34540..37143
                     /locus_tag="U3989_000180"
     CDS             34540..37143
                     /locus_tag="U3989_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1713655.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            37260..38909
                     /locus_tag="U3989_000181"
     CDS             37260..38909
                     /locus_tag="U3989_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1713656.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            39225..40160
                     /locus_tag="U3989_000182"
     CDS             39225..40160
                     /locus_tag="U3989_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1713657.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            40153..40923
                     /locus_tag="U3989_000183"
     CDS             40153..40923
                     /locus_tag="U3989_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713658.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT        12507 a         7853 c         7687 g        13034 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaataa
    19501 ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat cttttattat
    19561 gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct ctcttgattt
    19621 gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa tttcacgttc
    19681 ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg tcactcgaaa
    19741 atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa aagttgcatc
    19801 cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta ccaaccaaga
    19861 agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact gggtgcgaaa
    19921 gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa tggcaacctc
    19981 tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa cttcggggga
    20041 tttccctttg ataatataag aaataaactt atccttattt gcttttgcat actctaaata
    20101 agaatcatta ctttccatat taattatccc ctttcttcct actatataaa agtattatac
    20161 gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt ttctaattta
    20221 ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa ctcactaatc
    20281 acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa gaagtttttt
    20341 tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa agccacgcga
    20401 aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc
    20461 aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa
    20521 atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt
    20581 aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt
    20641 tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa
    20701 atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca
    20761 tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg
    20821 aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa
    20881 tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg
    20941 tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct
    21001 ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt
    21061 aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct
    21121 ttcattcgat aacagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc
    21181 aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga
    21241 atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga
    21301 atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct
    21361 tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta
    21421 aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga
    21481 acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg
    21541 atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt
    21601 tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa
    21661 aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg
    21721 ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca
    21781 gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat
    21841 aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc
    21901 gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt
    21961 cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa
    22021 gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt
    22081 ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg
    22141 taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa
    22201 attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac
    22261 ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat
    22321 accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa
    22381 cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt
    22441 cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac
    22501 cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac
    22561 cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt
    22621 tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt
    22681 tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac
    22741 gttggttctt aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat
    22801 aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt
    22861 tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa
    22921 cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta
    22981 aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat
    23041 cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt
    23101 ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag
    23161 atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag
    23221 cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca
    23281 catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa
    23341 catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg
    23401 ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga
    23461 caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg
    23521 ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt
    23581 catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc
    23641 agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca
    23701 gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt
    23761 cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca
    23821 gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg
    23881 taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt
    23941 ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc
    24001 cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg
    24061 tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa
    24121 aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga
    24181 aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat
    24241 ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat
    24301 atccttcggg cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg
    24361 caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct
    24421 tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa
    24481 acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt
    24541 tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac
    24601 tggtaacagg ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg
    24661 tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg
    24721 cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa
    24781 cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat
    24841 tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt
    24901 tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca
    24961 tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg
    25021 taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg
    25081 caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt
    25141 ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt
    25201 aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg
    25261 cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat
    25321 tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat
    25381 tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg
    25441 attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt
    25501 caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc
    25561 gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa
    25621 tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat
    25681 cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga
    25741 ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa
    25801 cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg
    25861 ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg
    25921 taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata
    25981 atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat
    26041 aagatgctcg cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat
    26101 agaatggatt tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa atatactgcc
    26161 tttaaattag aacacatttt agaacacaaa catttcaaat ttaatcaaat ataataaaat
    26221 atatttacgg gaaaaccttt ataaatcaag gtttttcaaa cttattcaat gatacgaaga
    26281 aaaaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
    26341 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
    26401 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
    26461 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
    26521 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
    26581 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
    26641 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
    26701 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
    26761 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
    26821 ggcttcagcg agaagttcca acgcatatac aaggacgact tatgtcaacg ataacaacga
    26881 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggaaga
    26941 caacgcaacc acgtctgcga gacagtctaa tttttctggg agggagtctt gctatagtta
    27001 tccttataac cggtctggta aaattactca agcttgatct gaaagaagca aaaattttca
    27061 gtgaagagga atacagagaa acgactttgg tcgaaaagaa agaatcagga aactgataca
    27121 agcgtataac aaactgcctg ataataggaa agacacgtat tgtccgattg ttttttttga
    27181 cattcaattg gaaaatttac actttcttta tacatttctg gctgctttct ttatacccgt
    27241 caactatact tcactcatca ataagaaaga aggataagta tggattttgg aaatgaaacg
    27301 gtcttgaaag caactggtat aaccaaaaaa tacggtgctg ccaaagcatt agataaagta
    27361 tcaatcgaaa tcaaacgtgg aatgatctat ggattgattg gggaaaatgg agcgggtaag
    27421 tctaccttta tgagaactat tatgggtttg atctcgattg atgaaggttc gatcgaatta
    27481 tttggaacga cggatttgca ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct
    27541 gctctttatc cagaattaac tgcaagagat aacctacgta tccaagcagc gaacggcggt
    27601 gtgtcggata gagaaatcga agacctgctg aaaatgatgc gattggaaaa tacagggaaa
    27661 aagaaagcaa agaatttctc tttaggtatg cgccagcgtt tagctattgc aaatgcactg
    27721 atcacgaatc cagaattttt gattttggat gaaccgacga atgggatgga tccagcaggg
    27781 atggcagaaa tgcgagaaat catccaacgt ttggtaaaag aacggggcat caccgtcttg
    27841 ctttctagcc acttgctaga tgaattgtca cagatcgcca ctcactatgg tattttgcat
    27901 gaaggacact tgatcaaaga attatcgaaa gaagaattgg cacaagaatc tcgtcaattt
    27961 ataaaaattg acacttcggc gacggaacaa gcagtaacgg ttctcgactc tcttggttat
    28021 agggattatt ttgtacaatc tagccgtgtt atccagcttt ttgaaggaat tgaccaagta
    28081 gcagccatca atcaagcatt ggtagaagca aaagttcctg tggatggtat tcacctagtt
    28141 gggcaaaaac tggaagatta tttcttacaa ttaactggag gcaatccaca tgtttaatct
    28201 attaactgct gaaaaaataa aagtatggaa gagccgtaaa atgtgggtct cactaggact
    28261 gatggtcgta cttccgcttt tttattcttg gaacgaatgg tatatgcata caaaatacgg
    28321 caatgaatta aaccaagcaa cggatacagt catcaacggt gcaacaggga ttttaatggt
    28381 agaaaaaatg gctgcatgga ttcttctggc tttcgcagca tttgcgtgct tttatattgg
    28441 ggaagaattt caaaacggta cgattcgcaa tacgttatct ttaggacgga atcgaatgac
    28501 ttattatgtg tcgaaacttc tcgttacgct tttgatcaca acacttggcg tggttttgat
    28561 cacgggacta gccatgcttg cttacacact tgctttcgga tttggtgaag ttgagggaat
    28621 caaaaactat ggaaattatg ttctaaaagt atttcctgta ttgcttctgc tgacattagg
    28681 gacattatct gttccagtgg ctttgacatt catcactaga agtaccagtg tcagtttgct
    28741 tctttctttt ctttatatca tgggaacggc cttcattccc ggtgttttcg caaaaatcaa
    28801 aggcttggaa tttctaacgg agtggtttac ggaaacgtgg ctcatgtata cggactttgc
    28861 gcaacaagca acttattcgc aagcaccgaa aatgattctg gtcagtctag taacaatcgt
    28921 ggtatcatca gctctaggaa tgtttatttt ccaaaaatct gacattaagt aggtggtcct
    28981 gctggaaact attttgatta tcgaagacga tgaagctgtt cattccttac tggaggaggt
    29041 tcttgaacag agatacaaac ttcttgatgc ttattcaggt acggaaggaa aattattgtt
    29101 aagcacatat cctgtcgatt tgatccttct agatctcatg cttccagggc tttcaggtga
    29161 agcattgtta gcagaaatcc gacagacatc gaatgttccg atcatcgttc tttcagcaaa
    29221 aagcgatcaa agagataaag tttccttgtt ggcagcagga gcggatgact atgtgacgaa
    29281 accatttgat atcgaagaac ttttgttacg aatcggcatc caacttcgtc atcagacttc
    29341 tgtccaagta aaagatctgt cgcaacgcat ctattacaag gaaatcttac tggacaaaga
    29401 gtcgagggat gtaaatgtga atggtgtggc tgtccatttg acaggaagag aattcgatct
    29461 gctgaagctt tttttagaac atccgaaaaa agtattttcg cgagcaaatc tttatgaaac
    29521 agtttggcaa gaaccatatt ttgatagtga gaaaacagtg aatatgcata tttccaatct
    29581 tcgagggaag ctagcaaagg cacatgctgt gtatattcat acggtgtggg gagtggggtt
    29641 tagatttgat taaatggctg ttgcttctcg ttgttatctt tatgggaagt tttattttct
    29701 tcctgcaaag ggaaatcaag agattaacta agaaaatcgc cactctttct gaacgacaag
    29761 aatatggtgg tcgtctttca gtcgaatttc gagaaaagaa tctaatggac ctagtagatg
    29821 cactgaatac atggttggat gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag
    29881 aaaacatcag actatcgatc gtcggccttt ctcatgatct cagaactcct ttgactgcta
    29941 ttaacggtta tgtacaatta ctggaacagg tagaggatac ggagaaacgg gagaagtatt
    30001 taacgatcat caaacaatct gttgcaagat tactcagtat gacggatcag ttttatgatc
    30061 ttgctcgaat agagacgaag caaaaagaaa tggatctcca tacgctttct ttttctaaag
    30121 aggtggaaga agtatttttc tctttttatg aacaattttc tgagaaaaat atcgaggtag
    30181 cgttcgctga acactcgata aatacaactg tattagcaga tccgctcatg ttgctgcgag
    30241 tgctgcaaaa tatcatacag aatttattgc gttatgcaga tggacaagca aaggtgacat
    30301 tttcaaaaga ggatccattc attcgattaa cagtggccaa tcctatcaaa ccaggaacgc
    30361 aaacctctgt tgaaaaagtc ttccagcgtt tttataccga aaataccagc cgcacaaata
    30421 cggaatctag cggaatcggg ctttacctgt caaaaaaatt ggtggaagga atgggaggag
    30481 agatgaccgc tagattagac gggggcatct tctccatctc cgtcaagttg cggagggttt
    30541 aattatacaa gattagtgag aactagtaga aaaatgtatt atcttatata tatttaatag
    30601 agaaaaaagc ctgtgatata aatattgcag gcttttttat tgatttgaaa taatcaagaa
    30661 tattagaaac ttctacatta atcaagaact ttcttctgta tgaaaataga gataataatt
    30721 gtacacttaa gctgtattca ggaggtgaag ataatgggca tcgaatttag cgaacggcaa
    30781 atgaaaatac tatacttttt gattgaaaat gagcacacaa cttacaaaga attatcagaa
    30841 caattatatg tatctacgaa aacaattagc aaagatattt taattttgaa agaaataatc
    30901 caaaaaatgc cagatgtaca aatgttggtt caacctaaaa aaggcgtttc gcttattgga
    30961 aaaaaagaaa cacttcgaaa tttattatcc agtcataaaa tcaatatcgc agactctccg
    31021 gaggaccgtc taacttatgt gtttgctaaa ttattgaata ctaatgattc tctaaagatt
    31081 caaaatcttg ctgacgaatt atacgtgagc aggtcgacgg tagaaagcac aatgaaagat
    31141 gttagaaaaa gactaaacaa gcatgggatg tccttagttt cagatcgaaa tggcgtgcgc
    31201 atcaaagctg atgaaaaaag taaaagaagg ttgatgtcag aagttgtgaa ctattattgg
    31261 ggtggcttta cagcatctaa cagtaaaaca gatctcttag acttgaatat caatttcatt
    31321 ggtgaagcga ataatttctt aaataaggac atattgaaaa aagtagccga attgcttaat
    31381 cgatacatga ataagacaca attgaaattt acagattatg aatttcaaac tttagctatc
    31441 caccttgcta ttgcgataga aaggattgaa acaagcaatt tcttatcacg tagtaataat
    31501 tctgaagaag aaatctcaaa gaatacactt attttaatca aaatgattga aaaagcattt
    31561 tcaattattg tgccaataga tgaacaagct tatgttgaca ttcacattac ttcgattgaa
    31621 aaaaatgcta tgaatcacga cttagatcaa acgaagcttt cctatttaga tatgggtgtt
    31681 gagctacaag agttagtaag aattaaatta aatgaatttt atcctgatga agaattgatc
    31741 aataatctag tattacatct caatgcagca gttaaaagac tcaaattggg agtaaatatc
    31801 tataaccctt atacggacaa aatcaaatat tcttttaaaa ggtcatttat gataagtgta
    31861 gatttattag aggaaatcga agagagattc tgtatacatt ttaatgaaga tgaaatcgct
    31921 tacgtaacac tccatattca atcacttttg gatcgttaca aacctgacaa aacgaaagtg
    31981 atattagttt gtagtagcgg atatggaaca tcaaaattat tagagcaaag aataaataac
    32041 ggttttgcag atatggttga gatagaagat gtactaagca tcaatgaact acaagataga
    32101 aaagtcactg atgaacttgt tataagcacg attccgattg aaaatacaaa ttttcctgtt
    32161 attgttgtta gtcctttgat gttaggggca gatgtcagaa aagtaaaaca gtatctacag
    32221 ttaaatgaac aaacagaaga tgcttttcta aatttattag ataaacgatt ttgctttttt
    32281 acccaccaaa taaaggataa tcaagtgaac gttttaaata taattaccaa aaaattggta
    32341 aaagaaaatt acgcgaaaga agggatacta gagggagcaa tcaaaagaga aaaactatct
    32401 tcaacagcga tgaatgattt tgctatgccg catatcgaag tagattttat aaaaaagcca
    32461 gtaatctgtg tctacgttaa tccagaagga attgattggg atgggacact tgttcattct
    32521 gttttctttt ttgcacttaa tgaaaaagta aaacctgaat tgaataaaat ttatgaaaca
    32581 ttcaatgaaa tcgcttcgaa tcatgagtta ttagcaaatt taaaaacgag ttcgtcgttt
    32641 gaaaaattag tttatatatt gaaagaaggc ttataaatga tcagtgaaaa aatcaatata
    32701 aataatatag tgatagattt aaatgcatct gataaaaaaa gtgtagtcga aaaattatcg
    32761 aaattgttag agactaatgg gtttgtgtct gaccacaaac aatttcaaaa agacgtattt
    32821 gacagagaaa accatatgac cacaggaatt gggaaaaata tcgccattcc tcatggcaaa
    32881 agcctagcag taattgaatc aacagtggct gtagccaagt taaagcggtc tgttgaatgg
    32941 ggcgcattag atgatcaacc agttgatctc gtctttttgt tagcaataaa agacactgat
    33001 aaaggtacta atcatttacg tattttagca gaattatctg gaagattgat ggatgacgag
    33061 tttgttaaaa gaataaaaga ttcgagaaat aaaaaagaat tggtaaaagc tctatcattt
    33121 taaaaggagg aatttcatat gacaaaaata gtagcagtaa cagcatgtat cgcagggatt
    33181 gcccatactt atatggcaaa agctaattta gaaaagtttg cgaagaagaa agggttagat
    33241 attaaagtag aaacacaggg ggctatgggg atagaaaaca aactttcaat ttcggatgtt
    33301 tccgaggcag atgttgtcat atttgcagta gatacaaatg tttcagaacg aggtcgcttt
    33361 gacggtaaac cgattttaac agtagggacc tcagaggtag taaaagatgg tgaagagacg
    33421 attgatgaag caattgcatt aattaacaaa taaaaaatat atcgtggggg agttactaat
    33481 ggaattcaag aaacaaggaa gagaaattct aaatcatttc caaagtgggg tttcctatat
    33541 gatcccgctt gtcgtagcag cagggttact aacttctatt gcagtcattt ttggtggcac
    33601 aggtgtttgg gaccagacgg atacattctg gggagtcctg cgaatgattg gtcagacggg
    33661 attacagttt atcgtaccga tgatttcagc ctatatcgca tattcaattg ctgaccgtcc
    33721 cggtctagca ccagctttta tcactgggat gatgtgtcaa aatcttggta tgggatttat
    33781 tggaggaatg gttgctggat tagcttgtgg ctatttagct aatgcattga aaaaagtaaa
    33841 agtaccgatg caagttatta gtctaaaatc aattatgctg attccttttg ttactacatt
    33901 agttgttgga atcatattat ggtatgtact agctacgcct attcaagcaa tgacaacagg
    33961 actgactaat tggctagaag gcatgtcagg agcaaatgct gctttgatgt cggctatttt
    34021 aggaggaatg atggcgtttg atatgggagg acctatcaat aaaatcgcat atgcttttgg
    34081 ggttgcttct ttttcaagcg gaagctttgg acctagtaca gccatgttat taggaattgc
    34141 tattccgcca tttggaatgt ttttagcaac aattttaaac aaaaaacttt atgatgaatc
    34201 ggaacgagat aatgcaaaga cagctatgat tatgggcctt gtggggatta ctgaagggac
    34261 gatcccgttt gccgtgaaag atcctttacg agtaatacca agcatcgtgg tgggcactgc
    34321 agtagcatgt ggtctaaatg gattttttgg tgttactcaa gaaacaatgc tttcaacctt
    34381 tatggcaatt ccttttgtta cgaatatacc tttatattta gtatcaattg ttattggggg
    34441 agtggttaca gcgttaatgg tcaatgcact aaaatccatc aagtataaaa aagaacaaca
    34501 agtaaattca gtagaagaag gaaagtagga gaatctgata tggtaaatgc acatataatt
    34561 caacacacgc actgggacag agaatggtat tttacaaatg aagatgccat cgtattgtct
    34621 gatcaggtct ttactgaagt tctagatgaa ttggaaagga accctaaagt aaacttttgt
    34681 ttagatggac aaacttcaat cgtcgatgag tatttggaga tcaatccgca aaagcttaag
    34741 gttatccaac gactgatgaa aacaggacaa ctattcgtgg gaccgtggta tgcacaaaca
    34801 gatgccttat tagtggatgc tgaatcaatt ttaagaaact taatgattgg aatcagtgat
    34861 acgatcctta aatatggaga tccaatgatg gtagggtatt tgcctgacac gtttgggttc
    34921 aatgcgaatt tgcctacact tttgcatcaa gtagaaatcg ataatttcat gtgttggcga
    34981 ggattaaact ttgaaacgat ggttccatct ccttatttta tctggaaagg attgggagat
    35041 aaatttgttt atgccatgaa ttttccattt ggttatatga ctggaatgat gaccttagac
    35101 gcaattaaag atattcctga atttgcaaaa gaaaagttgg atgaagcagt tgaaatgctt
    35161 catatgcaag gaaaaaacaa agatatttta gtaccttcgg gtgtagatca aaagtcaatg
    35221 attttaaatt ttgatcaagt cgtagagaag ttgaataaat acagtaaatt ccacaatatt
    35281 atttcagatt atccttctta tgtagaagtt atcagaaaca aaaaagaatt acctgaatat
    35341 aaaggtgaac ttcgtcagcc ggtttactct cgtgttcatc gttctattgg ctcagtaaga
    35401 acaaaaatga aaatagaaaa ttttaaattg gaacaaaaaa ttctaaaacg agttgagccg
    35461 ctcatgctga ttgcagaaag aaatggtatc catatcagta aaggcctact tcgtcggtta
    35521 tggaaaaaga ttttgcaaaa ccaggctcat gatagtattg gtggctgtgt ttcagataat
    35581 gttgcggaag atattttcca tcgaatcaaa gaagctaatg agatagctga cggaatcgaa
    35641 aaccttattg taaaacggat ggctgatgca ttaaagctaa aatcgaatga agtccttgtt
    35701 ttcaacacaa cgactgagaa ttatagtgga tcgaaaatcg tgcatgtagt agctgattca
    35761 aagaaaatac acttcaaagg tagtccctat aatatcattg aaaaagaagt ttattttcca
    35821 gaacggaaaa atgcttatcg gatgattgct acaggtttcg aatattttga tgaaccagct
    35881 tattatgaac ttgacataaa aataaatgta aatgtgccag cactaggata cactgttgtt
    35941 gaatttgaga aaaataatga aaaattagaa acagcaatcg agatagacaa aactgagata
    36001 agtaacaata aatataaact ttcttttaaa gatgggcaat tgaatttgat agttggagat
    36061 agacaatatc ttgatttcgt tcatttgatc gattcagcaa atgatggcga tacgtatgat
    36121 tattcaccat tagaaggaga tacagaacta tctctaaaat tagaaacagc taaagtatat
    36181 aaagattctt tacaagaaac attagttgtt tacggaaaag ctcaacttcc taaagattta
    36241 aaagatcgtt tatctgaaaa acccgaaatg gaagaaatat cttatgaaat atcgttcagt
    36301 ctgggagaaa gtcaaatcgt tgaaggcaca ctcaaaattc ataatactgt ttactcgcat
    36361 cgtatgcgct tagcaattaa tgttttagat aaatctgaaa aatcaattgc acaaattcaa
    36421 aacggattta ttgaaaatac ccctgatgtc attcctccga attggtcaga aactatggta
    36481 gagaaacctg ttaatttaga aatattcgat aagagtgtct ctgtggcaga aaatgatcat
    36541 tattttacgt tttatgcaga tggtttaaaa gagtatgaac gtatagaaaa tcaattaatc
    36601 ataaccttat tttcaacaac aggggaactt ggaaaaccta atcttgcttg gcgacctggg
    36661 cgagcttcag gagatacgac aaatgaaggg catgtcatga tggaaacacc gctagctcaa
    36721 gagataggtg agtataaagt cacttttgga tttaatgtag aagaaggaag atttaatgag
    36781 ttcaaagtgg caaaacaggc agaaaaacgg ttagaacaat ctatttctta tcaaaagcag
    36841 aaactgaatg tatttattca caggttagat aacaagattt ggccattaca aaatgaactt
    36901 gatgttcctc gtaaattttc attgattgaa ttacctgaag acttaattgt atcatcgatt
    36961 tatccttctt attttcatga acaaggattt gttcttcgct tagctaatcc aacagaacaa
    37021 gaaaaaatag tacctgaggc tatcctttcc cttgggacag ttgtcaatgc actggagaat
    37081 aaacaagaat taacaacaat tcctccctat gattatttgt caattttgat aaatgagaga
    37141 taaatatgta atatacaaga aaaaaacacc ttttattttg aaaggtgttt ttttaccctc
    37201 ttttcaaata gcgcaacagt gctatactaa agctaacaaa taaaggaacg gagagggata
    37261 tgggtcaaag agaatttatc gttttaatta ttattgcagc tgttatttta ttagttgctt
    37321 tagacatttt ttcacgtttg aaattaaagg aaacagttcg tagcaaatgg gaaaaaattc
    37381 cttatcaacc gagatttgat aaagaagaaa gcttgaagga agcatggctc acggagaaga
    37441 agttccgttc atgggatagc gaaatcgatg atcttacttg gtatgacctg gatatgttcg
    37501 aagtattcga aggaatcaac agtacctatt ctagtgtagg ttcagaagcg ttgtatcaac
    37561 gtttacgcag ctttgatttt ggtgaagatc agcagttaga aaaactgatt gctttttatc
    37621 aggaaaatcc tcaactgaga gaaaaaatcc aatatcaatt cgctcgttta ggaaaaaaag
    37681 atcataattt tgcaaaacaa tatctagcgg atggaaaaag taaaaagatt ggacgcgtag
    37741 gtttatttat ttttcttggc ttgcttccgc tggtgggttt gggtttgctt cttttaggac
    37801 aagtactagg aatctatttg gcaatcggtg ggttagtatt caatacggtc tattatctag
    37861 tgaagaaaca aacactagaa acagagctga atagcatgag ttatttggtt tcaacgattt
    37921 cagccgccaa tcagctagca aaactgccga caccacttca agaagagctg aaacagcaaa
    37981 ctaaaccatt aagagacatc cctaaattag gtttttcatt tcgtatgaag agtgggagtg
    38041 aagcagagtt gatttttgaa tatgtcaaca tcatgttcat gctgccattt atctcctatc
    38101 atttcgttct cactaaatta gaaaagcatt caactgaagc catcaagctg tgggaattgc
    38161 tgggagaatt agaagtagcc gctgcaatat tgaattaccg tacttatatg cccatcactt
    38221 gccgaccaga gtttactgag ggcggtgtga cagcgaaaga tatctatcat ccattgttaa
    38281 aagaagcggt agtgaatccg gtcaactggc agaagaatac attagtcacg ggttccaatg
    38341 catcaggaaa atctacgtat gtcaaaagta tagccatcag ctgtattcta gcacagacga
    38401 tccaaacagc gattgcagaa cagtttaccc tgcaacctgg acatgtgttg acttccatgg
    38461 caatcgaaga tgatttattt gaaggcgaca gttattttgt ttcagaaatc aaatcgatca
    38521 aacgtttgtt ggaccaagtg gcttccaaag aacgctgcta ctgcttcgta gatgagattt
    38581 taaaagggac aaatacgatc gaacggattg cttcttccgc ttcagtcgtt cggtggcttg
    38641 cggagtatcc atctttagca tttgtcgcca cacatgatat cgaattgaca gagattttaa
    38701 agaattattg tgagaatgtc cactttgaag aacaagtaac agaaggaaaa ggaatcagtt
    38761 ttgatttcaa attaaaagaa ggaccttcaa ggacccgaaa tgcgattgcc ttgctgaaag
    38821 ttttggatta cccagagact ttagtagaaa ccgcaaaaac ggaagccgcg ttatttgatg
    38881 aaaagcggca gtggcatgta ttgggatagg cgaagtgatc gaaagtattt attggatcaa
    38941 aaatcttggc ttttgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
    39001 ttacttttgt ctcacgctcc ttttgttgca catatgaata gtaatccttt atcttcgatt
    39061 tgttttttct gaaaaggttg tctgacttcg tccgattgat gtctttcatt aggagatttt
    39121 cctgagccca gcaacctgat ttttcgctgg aaccaagttg ttttttcatc tgctgtattc
    39181 catgatacaa taaagaatca tgtctataag gagataggaa ataaatggca aatgcgcttg
    39241 aaataaataa tttgaaaaag gtttatggaa gtggggtcga agcattgcga ggaatcgatc
    39301 tttccgtgaa agaaggagat ttctatgcgc tattaggacc aaatggagct gggaagtcta
    39361 caacgatcgg aatcattact tctttagtca ataaaacatc tggaaaagtg aatgtcttcg
    39421 gttatgatct ggatacggat ctagtccgag caaagcaaca gattggatta gtcccgcaag
    39481 agttcaattt caatcccttt gaaacggtcc agcaaatcgt cgtgaatcaa gcaggctact
    39541 acggtgtctc tcgcaaagaa gcgatgaaac gcagcgaaaa atatttgaag cagtccaatc
    39601 tttgggaaaa gagaaatgtc cgtgcacgta tgctttccgg gggaatgaag cgaagactga
    39661 tgattgctag ggcattgatg catgaaccac gtttgctgat cttggatgaa ccaacagcag
    39721 gagtcgatat tgaattgaga cgcgagatgt gggagtttct ccgagaattg aacgaaaatg
    39781 gtacaacgat cattttgacg acgcattatt tagaagaagc tgaaatgctt tgtcgcaaca
    39841 tcgggatcat ccaatcaggt gaactgatcg agaatactag tatgaaagag cttctatcca
    39901 aactgcaatt tgaaacattt atctttgatt tagcgccata tgagaccaaa cctgtgattg
    39961 aaggatacgc ttgtacattt gaagatgaac agacaatcgc tgtcgaagtt gaacgaaatc
    40021 aaggggtgaa cggaatcttc gatcagctga caagacaagg aatcaaagtt ctttctatga
    40081 ggaataagtc gaatcgttta gaagaactgt ttttgaaaat cacagaagaa aataatcata
    40141 cggaggagaa aaatgtttaa tctttatttt actgcattaa aaagtttagc tatcaaggaa
    40201 acgaaccgct atttgcgtat ctgggtccaa acactggttc cgcctgtgat cacgacatcc
    40261 ttgtactttg ttattttcgg aaaaatgatc ggtggtcgga tcggtgacat gggtggtttt
    40321 tcatatatgg aattcatcgt gcctggtttg attatgatgt ctgctatcac tagttcgtat
    40381 gccaacgttt cttcgtcctt cttctctcaa aaattccaga aaaacatcga agaaatcttg
    40441 gtagcgcctg ttcctacgca tgtgatcatt tggggctttg tgattggcgg agtgggtcgc
    40501 agtattctcg tcggctctct cgtaacgatc atttccttgt tctttgtacc acttcatgtg
    40561 tattcatggt tcatggtcat cattacatta ttgatgacgg caatcttgtt ttcgttagca
    40621 ggactgctca acgggatttt tgctcagtct tatgacgatg tatcgatcgt gcctacattt
    40681 gtcttgcagc cgttaactta tctaggtggc gttttctatg ccatctctat gttgccgcca
    40741 ttttggcaag ctgtttcgaa agtcaatccg atcgtttata tgatctccgg tttccgttac
    40801 ggattccttg gaacaatcga tgtgccagta gcaacatcga tgatcgtact tgtgctattc
    40861 atcgttgttt tgtacgccgt tacttggtat ttgatcaata aaggaagagg attgagaagt
    40921 tagaaaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacgggaaaa
    40981 tagttcctca tattttgtcg tttgttgagc taataccgag ggtcagctga ttgaacaccg
    41041 tttagtggag aggttgtgaa aaagctgttc tgcatcaagt a
//
LOCUS       DASJON010000005        40813 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJON010000005 DASJON010000000
VERSION     DASJON010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.005"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(191..610)
                     /locus_tag="U3989_000184"
     CDS             complement(191..610)
                     /locus_tag="U3989_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN1713659.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(612..1196)
                     /locus_tag="U3989_000185"
     CDS             complement(612..1196)
                     /locus_tag="U3989_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN1713660.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1243..2718)
                     /locus_tag="U3989_000186"
     CDS             complement(1243..2718)
                     /locus_tag="U3989_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN1713661.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2899..3210
                     /locus_tag="U3989_000187"
     CDS             2899..3210
                     /locus_tag="U3989_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713662.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3325..5661)
                     /locus_tag="U3989_000188"
     CDS             complement(3325..5661)
                     /locus_tag="U3989_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1713663.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5803..6471
                     /locus_tag="U3989_000189"
     CDS             5803..6471
                     /locus_tag="U3989_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1713664.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6474..7517
                     /locus_tag="U3989_000190"
     CDS             6474..7517
                     /locus_tag="U3989_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1713665.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7754..8635)
                     /locus_tag="U3989_000191"
     CDS             complement(7754..8635)
                     /locus_tag="U3989_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1713666.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8794..9522
                     /locus_tag="U3989_000192"
     CDS             8794..9522
                     /locus_tag="U3989_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN1713667.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9678..9995
                     /locus_tag="U3989_000193"
     CDS             9678..9995
                     /locus_tag="U3989_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1713668.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9988..10596
                     /locus_tag="U3989_000194"
     CDS             9988..10596
                     /locus_tag="U3989_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN1713669.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10651..11442
                     /locus_tag="U3989_000195"
     CDS             10651..11442
                     /locus_tag="U3989_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN1713670.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            11580..12068
                     /locus_tag="U3989_000196"
     CDS             11580..12068
                     /locus_tag="U3989_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN1713671.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12119..12991
                     /locus_tag="U3989_000197"
     CDS             12119..12991
                     /locus_tag="U3989_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1713672.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13167..13529
                     /locus_tag="U3989_000198"
     CDS             13167..13529
                     /locus_tag="U3989_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1713673.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13538..14290
                     /locus_tag="U3989_000199"
     CDS             13538..14290
                     /locus_tag="U3989_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1713674.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14304..16106
                     /locus_tag="U3989_000200"
     CDS             14304..16106
                     /locus_tag="U3989_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713675.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16383..17423
                     /locus_tag="U3989_000201"
     CDS             16383..17423
                     /locus_tag="U3989_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1713676.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17475..19619)
                     /locus_tag="U3989_000202"
     CDS             complement(17475..19619)
                     /locus_tag="U3989_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1713677.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(19976..21349)
                     /locus_tag="U3989_000203"
     CDS             complement(19976..21349)
                     /locus_tag="U3989_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1713678.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21349..21744)
                     /gene="rbsD"
                     /locus_tag="U3989_000204"
     CDS             complement(21349..21744)
                     /gene="rbsD"
                     /locus_tag="U3989_000204"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN1713679.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(21772..22689)
                     /gene="rbsK"
                     /locus_tag="U3989_000205"
     CDS             complement(21772..22689)
                     /gene="rbsK"
                     /locus_tag="U3989_000205"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN1713680.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(22797..23810)
                     /locus_tag="U3989_000206"
     CDS             complement(22797..23810)
                     /locus_tag="U3989_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1713681.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(23891..24490)
                     /locus_tag="U3989_000207"
     CDS             complement(23891..24490)
                     /locus_tag="U3989_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1713682.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            24676..25377
                     /locus_tag="U3989_000208"
     CDS             24676..25377
                     /locus_tag="U3989_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1713683.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25387..28098
                     /locus_tag="U3989_000209"
     CDS             25387..28098
                     /locus_tag="U3989_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713684.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            28549..30177
                     /gene="treC"
                     /locus_tag="U3989_000210"
     CDS             28549..30177
                     /gene="treC"
                     /locus_tag="U3989_000210"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN1713685.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30237..31418)
                     /locus_tag="U3989_000211"
     CDS             complement(30237..31418)
                     /locus_tag="U3989_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1713686.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            31670..33193
                     /locus_tag="U3989_000212"
     CDS             31670..33193
                     /locus_tag="U3989_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1713687.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33262..33990)
                     /locus_tag="U3989_000213"
     CDS             complement(33262..33990)
                     /locus_tag="U3989_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1713688.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34003..35283)
                     /locus_tag="U3989_000214"
     CDS             complement(34003..35283)
                     /locus_tag="U3989_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1713689.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(35424..36026)
                     /locus_tag="U3989_000215"
     CDS             complement(35424..36026)
                     /locus_tag="U3989_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1713690.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36271..36477
                     /locus_tag="U3989_000216"
     CDS             36271..36477
                     /locus_tag="U3989_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713691.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            36789..37508
                     /locus_tag="U3989_000217"
     CDS             36789..37508
                     /locus_tag="U3989_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1713692.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(37589..38272)
                     /locus_tag="U3989_000218"
     CDS             complement(37589..38272)
                     /locus_tag="U3989_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN1713693.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            38490..40676
                     /locus_tag="U3989_000219"
     CDS             38490..40676
                     /locus_tag="U3989_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1713694.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13233 a         7246 c         8174 g        12160 t
ORIGIN      
        1 tgtggaaacc catgtttaca aaatttttct tactactcga aacgaagcgg ctttatcaca
       61 acctcttgat aaacggcgtt caatcagctg tccctcggta ttagctcaaa aacggcaaaa
      121 tatgagaagc tatttttcca tttttttctc tcatactcag gtcataacgc tgatttcaca
      181 acctcttctt tcataatggt ttttcaccat ctttataata aactggttct ttgatatgga
      241 aatctatttt ttccaaccaa tctaaaatct tgtaacctaa ctcttctgac atcggaaaag
      301 aggcgttgat ttgtgcattg gagatatggt caacatggat accggaagtg atcgtacaac
      361 ttcccggaac ggcagttttg atgatcacag ctattttttc ggctaagaca tcgtctttat
      421 ggaaccgacc atcgtggcta gggaaacgta cagtttgcat ctctgtatca cgagaaaatg
      481 tagtgactgt tccgatatgt ggtgtatctc cgcctgttaa agtgatcaat aagtctttac
      541 cgattacttt aacagcagct tggatcgtga aacctagttt ggttacttca actatcgttt
      601 ctttttccat attaatcacc ctgccaacgt tttgaatgct catttacaat ttcaaattga
      661 tccaaaagtt tcattgtatg gtgattaaca agatcttgga tcgtttgagg ctgattataa
      721 aaagcaggaa ttggtggtat gatttgcaca cctagtcgtg ctaatttagt caagttttcc
      781 aagtgaatgg catttaatgg cgtttcacga ggaacaatga ctaactttcg ttgctccttc
      841 attgttacat cagcagcacg tgcaatcagg ttgtcgccta aaccaacaga gatacctgcg
      901 actgttttca tacttgccgg tacgatgatc attccatcgg tcaaaaatga tccgctggcg
      961 acagcagcac ctaaatcttt gtttgaataa tggtaatcaa acaggctttc gaattcagtt
     1021 agtgataagt cggattccag ctttaagttc tgttttgccc atggactgat gattccgtgt
     1081 gtttctatca tcggatattc ttttagtttc ttcactaggt tgatggcata aatggtgcct
     1141 gaagcgccac ttacaccaat cacgattttt ttcttccgtt gtgccaattg ttccattttc
     1201 cgcactcctt tttatgatgt caaaacagtt gcagatcatt cattatttca aatatttttc
     1261 ccaatcttct acttcttgga attttgcacg tacaaaatgt tctttcatat cgaaaggaac
     1321 tgtaccgtca aagatagttt ttgcacttat cccacggaaa cgaatagagc caggattata
     1381 ttctggtaat tctgatgggt ccaatggatg gttcctcatt cctgggatca cgatgatatc
     1441 tttatctcct tggaaacgag tgttgaaagt ccaaactaca tcgttcatgt cgaaaatatc
     1501 aacatcttca tcgactaaaa tgactgtctt caattctttg aaggctgata aagcaagaat
     1561 agctgcttga cgctgaatac cttcatccgc ttcgttttct ttacggattt gcatgatggt
     1621 catcaattta ccaccacctg caggaggatt gtagacattt actacttttc ctggaatagc
     1681 tttatcgacc aaggcaagaa tacttgcttc tgtaggaata ccggccatag atacgtgttc
     1741 ttcactggga ccaatcgttg tttgcatgat tggattgtct tttcggtgag taactgcttt
     1801 gactttcact acttgtacag ctggattagc atccccagta tagccaggaa attctggcat
     1861 tgcttttcct gagttcgtgt tgatatcttc ttgcattgtt tcatttggca tgatttctgc
     1921 ttcaatgatg aattcagcac gagcaatacc cacttcatcg actgctatac catcaacgat
     1981 ttgaataggt tcgttacgca aagctcctgc acaccatagt tcattgtagc caagtggagt
     2041 ggtaggtggt tcgaaagtcg tgccaatggc aattgctgga tctaaaccaa tattgatagt
     2101 gataggcatt ggtttattca aggcttcgaa ttgtttttgg aagtgaccaa tgtggcgtcc
     2161 gccaggcatg atgtacatac cgatcgtatc tttatcttct agaaccatac gatggatcgt
     2221 tacatcgctc attgtttttt ctggatctga tcctaagact acacccattg tgatgtatgg
     2281 accagcatct tcaggagtat ttgtaggtgc aggcaaaatg ttgcgaatat cgaagtctgc
     2341 atcggtagct agatgaacga cttcttgtgc tggagcgtct gctttgtcta ctttcacagg
     2401 tttgattgga ttttctacag cgtctttcaa gaaacgacca agtgttcgat aatcatggtg
     2461 caaaatttca cctacacgtt tacgactagc cattgttccg atgttcacac gtgtgccggg
     2521 gaaacctttg atattgttga aagtcatagc tggtccttct tgagtcggac gttttactgt
     2581 accgccagca ccgatataac ggtaaacacc agataattct gcttcaggat cgatttcaat
     2641 atctgtttcg tgatattgac ctggaagtgt ttttagttct tcgatgactt ttcttaaatc
     2701 atacggttga tctgtcatga gagattcctc cttagttgtc tttacctatc tatagtaaaa
     2761 ggtgaaaaag aggaaaacaa ttcaacttgt tcggtaaagt attctacaat ggaatggatg
     2821 cgtttttttg ataatagaat aaactatgat atacttatca ctaaaagaaa gcgcatactt
     2881 agtgttggag gaaggaaagt gaaaaaaatg gaggagtcat ttcctaaagc agtaaaagtg
     2941 gaaaatatag cgaatatttt gaaagttaca tttgaaaacg gagaagtaaa atacgtgaaa
     3001 tctcattgga cagaagagat aaccgatgcc ttgcaatttg gaaaaaaagg aagaggaaaa
     3061 agaaaaaatc ttctcgcact ttctcgtaat atgtggattg ggactgaagt aacgattgaa
     3121 gcagatggaa cagtattcat aaatggaaaa gacaggtata cgccagaaga gttgtggtac
     3181 aaaggaaaga aaagtatacc agaattataa aaaataatta aaaaaaacag cgcgaaacaa
     3241 ggaacatatt acttcctcgt ttcttgctgt tttctactat ttcagcgctc ttatgactca
     3301 aatctaaatg tggttgtcac aaccttattc tgcacgcaaa gcgatagcgg cgtctttttt
     3361 cgctgccatt cgtgcaggaa gatgaccgcc aagcatcgtc aatatagtag agacgatcac
     3421 tagaataatc gcatggacag gatttagttg agcaacattt tttagatccg tcatgttgta
     3481 taagatgcta ttgataggga acgtagctag ccatgcaata aacacaccta atatcccaga
     3541 agaaatacct aaaatacacg tttctgcgtc aaatacgcga gtgatgtctt ttttacgtgc
     3601 acctaaagct tttagtacac cgatttcttt tgtccgttca atcacagatg tataagtaat
     3661 gatactgatc atgatcatac ttgttactaa agagatccct gcaaaagcaa taaggacata
     3721 agtgatagca tccatcaatc cgccagtcag ttcagtcatc gttccagcta aatcagtata
     3781 aatgatttta tcttcatctg atttgccttt attgtaatca tctagataat ccaagatctt
     3841 ttctttgtct tcaaaattgt tcggataaat catgatactt gaaggcaagc tgtctccacc
     3901 taaataacta agcaatgttt gtttggcatt ttcgtcaact ttttcagtag ttaagacatt
     3961 tacaccactg tctctttgag ctttgacgat ttctgattct ttgttttcgt tgacgatttg
     4021 agtcgttaat tgatcgctat aagcgattcc tggagataag agattcattg tagaagaaga
     4081 tttcacgcgt aggatccccg agatcttcaa ttcatcagaa gcattctgat acatcgcatc
     4141 ataatctgta ttagggataa aattcccagt tggaagtttt gtatagtaag tattgttgtt
     4201 tactaatttg aatgttgtac caacgatatc atcaaaatca agtttttcgt cttctttcac
     4261 atcaaatcca agattcttca atgcgttgat attagtatta ttattgccgt ctacgatcag
     4321 cacgacatct gttgctgagg ctggataact tccggcaagt aaagaataat tgtcttttaa
     4381 aaagttttct ttgctagtgt caagctgtgt agggaaactt gacactccca cacctgtcat
     4441 tgctgacatc gtactagata aagaaagaga ttccgtatca ggattttgat tcgagaagct
     4501 gacaggctgt actttgccat ttacatcacg taaaagattg attcctgtag tacgggtaaa
     4561 tccgatatta ttacttaagt taggatcgat gtctgttacg taatcgacgt atgtttgatc
     4621 aatcttattc gtgtgctgtg ctttatcttc ttcgctgact tttgcagtaa tcgttttgct
     4681 gtctggataa tccgctttgc ttgcacccaa tccggcatcg tcacgagtct ggttagtcgt
     4741 taccttagaa atagtaatcg gaaatttcgc catcgtttca gcttgcgtgt tatcgatttg
     4801 tttttggaat ccagaagaaa gggacagaac gatcgcgatt ccgatgatcc caatactgga
     4861 agcaaaggag gtcaagaaag tacgtccttt ttttgttcgg atattgttga aggaaagctt
     4921 taacgctgtc caaaaactca tctttgttct gcgtaagtta aattgatcat cttttggtcg
     4981 ttcgatgtga ggatgggaat ctgttaagat cttaccatca gaaaattcaa tgattcgatc
     5041 tgcgtattga tgggccagtt caggattatg tgtgaccatg atcactaatt tttctttcga
     5101 taactcttgg atcaattcca tgatctggat acttgtttct gtatctaacg ctccggttgg
     5161 ttcatcgcac aaaaggatat ctgggtcatt ggctagtgct cgagcgatgg ccactcgctg
     5221 catttgtcca ccagataact gatttggctt tttatgcata tgatttgtca agccgactcg
     5281 gtgcaatgca tcttctgccc gttttcgttt ttcgtcttta gaaacaccac ttaatgtcat
     5341 cccaagttca acgttttcaa taatgcctag atgaccaatc aagttgtaac tttgaaaaat
     5401 gaaaccgata gagttgtttc ggtaggcatc ccagtcactg tccttaaagt ctttagtaga
     5461 ctttccgttg attaccatat caccggaatc ataattatct agtccaccaa tcacattcag
     5521 catagtcgtt ttaccagaac cactaggacc taaaatcgct acgaattctt tttgtctgaa
     5581 agcgacagac acaccatcca atgctttggt ggtggtttcg ccgactttat aatatttttt
     5641 tatatctttt aattgaagca taatcaccct tctcccgtat tgaaatattc ggtatactaa
     5701 taagtatatc agaacaatga tcgtagtata cgaaataaat gttaactgaa gatgaactac
     5761 aagaaaaaat ctagaaattt ttcaaaaaac agagggagat caatgaatcg tattttagta
     5821 gtagaagatg atccgcaatt acagttgctt taccgttctg tgttggaacg agtgggcttt
     5881 tcagtcatca ccgcattgaa tggagaggat gcgctgaaaa aactagaagc gacacgtata
     5941 gaattaatca ttacagatat catgatgccg aaaatggatg gctatgactt gctagactca
     6001 ttacgtagca ccagatttga cacacctgtt ctaatgatta cggcaaaggc cgattttgag
     6061 gataagaaac gcgggttcaa attaggagca gatgattata tgacaaaacc catcgatgtc
     6121 aatgagatga tcctacgtgt ggaagcattg cttcgacgtt caaagatcaa ttacagcagt
     6181 agcttgataa tcggacaaac aaaattagag ctagatgctt atacagtgtt acaagatcag
     6241 cattcgttga ttctgcctca aaaagaattt ctgcttctgt ataaattact gtcttatccc
     6301 aacaagatct tcactcgcca acagttgatg gatgatattt ggggattaga tacgaataca
     6361 gaagaacgaa cgatcgatgt acatatcaaa cgtttaagga cccgttttga aaagacggaa
     6421 gattttcaaa tcatcacagt acggggacta ggctataagg cggttattta aagatgaaaa
     6481 aaaagctatt ttcacaatta tggatctatt ttgctagtat tgtcttcatt tcgatcttag
     6541 tgacactgct gtgttttctc ggttttattt tcttattgtc tcgacattct atctcgcctg
     6601 ctgaatcggc tggaaaacca acgttatacc cgttattcgt ctttgcgggg ttcagtatgg
     6661 tcgtgggtac aggaatatcg atttttgttg ggcggcgtat cctgcatcca atcagtgcgc
     6721 tagggacaaa tatgagtctg gtagcaacgg gagatttttc gattcgaatg gatgaacagc
     6781 agaaagtagc agaagtacag caactgtata aagatttcaa cgttatggtc caagaactca
     6841 atagtatcga gacactgagg aatgactttg tgtccagtgt atcccatgaa ttcaaaacac
     6901 cactagcaac aatccaagga tatgttcaat tgttacaagc cccaaatctc tctgacgaag
     6961 aacgccaaat atttttatat cggattatcg aaagtatcac gcagctttca caattaacag
     7021 agaacatctt aaaattgaac aaactggaaa accagcgtat ccaactggaa aagaaagagt
     7081 atcgattaga tgaacaaatc agagaagtaa tcgttttttt tcagcctaaa tgggagaaag
     7141 aacagctaga attggatatc gaacttgctg cagtaaatta cacaggcaat gaagagtttt
     7201 tgtaccaagt gtggctgaat atcatggata acgccataaa gtacaatcaa atcaatggac
     7261 agattcatat caaactattt gagacagcca cagagattgt actggaagtg accgattcag
     7321 gtgtaggaat gaatgaagag accagagatc gaatgtttga aaaattttat caaggagata
     7381 caagtcgtca aatttctggg aatgggctag gactttcact ggttaaaaaa attctagaac
     7441 tgcatgatgg acgtattgat tatagcagta tcgaaggagt aggaacgaca gcaatgatac
     7501 ggttaagtaa acaataggaa aaagaataaa acccctaaaa attctattga aggtttttag
     7561 gggtttttag tcgttttttt gtgattaggg aaatgttctt acggttcttt tggcataaaa
     7621 atcgctgctt tcttattgaa ctacgagaaa tgttagaggt gtcattgaaa caaggacatg
     7681 atgcaagaaa gagagcgaaa gtcatgcgtc gctctctttt tttgggtgga tagatggaca
     7741 gaaaatgtag ggcttagttt tcttcttgat ttaaatactc ccaaaatgta tctgaagcca
     7801 aagaatgctc accgacttta cgagtaaatg caagataact ctctgttgtt ggagaaatag
     7861 ggagaatcac agtattttct tttaatagat tcttgactgt tttttccata acaatagcaa
     7921 tccccatgtc atttgcaatc atattgatga tcaagtcgat tcttgctcct ttataagaga
     7981 tttttggttc aaatccagct tcgcgacaca gatctagtac aggttgatac aagttcgtag
     8041 tggggccaag gagcaaaaag ttctcctttt tcagttcgct tagatccaat ttctttttac
     8101 tagctaaagg atgattttta ggtagaactg ctacaaaatc gtctgtttca gtaggcaacc
     8161 actcaatcga acttggtggg gcctggaaac ttctagcaaa gtggatcgtg tcactatgtt
     8221 cattggcgtc attgtaagtt aattcgacac tttccacttc tttcaactgg actgtaaatt
     8281 caggatggcg tttcaaaaac tcggtgattt tggaaaaacc tttataattg atcattgtcg
     8341 gtatcgttaa gatatttaat gaaagattgt tgtctttcgt ataggtttca agcacttctt
     8401 gcatagcatg gtattgttct acgacatttt ttacatatgg gagcaataag cttccagctt
     8461 cggtcaattc gatttttcta tgggcgcgtt cgaataattt gacgtctaat tctgtttcta
     8521 acgaaagaat ctgtttagaa atatttcctt gggtggtgaa ttgtcgatca gcagttttgg
     8581 tgaaattcat tgtttcggct aaatcaagaa aaacttcgaa tttatggata tccatcatat
     8641 tcctcctact tattccaata aagaatactt taacacaaaa aatgaattgg tgtaatcttc
     8701 tctgtctgtt acaatctttc ttgcagcgaa agcaataatt acagaaattt ttctgatttt
     8761 tttcgcttcg tagaaaaaga gaggagtttt ttgatgccaa ctggagtttt gatcaatgta
     8821 ggttccgttt tgtttggcgg actaattggc ggattagtag gaaataaatt aagtgaacat
     8881 tttaaagcac agctgacgat ggtttttggt gtgtgttcga tggggatggg aatctattcc
     8941 attgcgccga tgaaaaatat gccggctgtt atttttgctt tagtgatagg aacagcgatt
     9001 ggtttgattg ttcatttagg taatgggata aataaggggg ctgcgctgat gcaagtacct
     9061 atttctaaaa tcttccctag tgaaaagctt ggtatgaccc acgatgaatt tatcagcaca
     9121 ttagtgacgg tcattgttct attttgtgca agtggtacgg ggatctatgg aagtttagat
     9181 tctgggatga caggagacag tacgattttg atttctaaat ctgtccttga ttttttcaca
     9241 gcagctattt ttgcgtgtaa tttagggtat gtcgtttctg tgatagcaat tccccaattc
     9301 attatttttt atattttgtt cttattggca aaatttattt atccattgac tacaccagac
     9361 atgatcttag attttaaagc ttgcggcgga ttcttgatgg tcgctaccgg ttttagaatg
     9421 atcaacgtga aaatgttccc agtagctgac atgatacctg caatgatcgt tatcatgcca
     9481 ctgagctgga tgtggacaaa ttggattatg ccactgttat aataaaaatc aaagagaagc
     9541 cattttttgc tgactaaaaa ggatcaaatc ttttagtcag tttttttgta tttgacaaac
     9601 tgattttgaa actatattat atgttgtata atatagattg aaataaaata ttacacttga
     9661 gaatagaagg tgacgtaatg cacatccaga ttccaactgt tcttcttgat ggtactgtgc
     9721 tagcagcatt aagcaaggaa gatatgtatg ggtatgcctt aacaaaaatc gtacaagaaa
     9781 gtctctctgt cagtgaatct acactatatc cggttttgag aagattgaaa aaagaaggat
     9841 atcttgagac ctatgatgaa ccttttgaag gcagaaaccg tcgatattac tcgttgacac
     9901 caatgggaag tcgtcattta gaagaaatta ttgatgaatg gattgttttt cgagattctt
     9961 tagaccggtt attggaggag agcacaaatg aatgaatatt tgaatgaagt tgtggatcat
    10021 cttcaagaaa ttcctgaaga cgatcgtttt gatttgatcc agtattatga ggaatatttt
    10081 ttagattctg gaaaaacatt agatcagatc attgatgaat atggtacacc aaagcagttt
    10141 gccttaaaat taaaaatcag ttacttttca gaagaagaac aagcagaaaa cgagttagtg
    10201 aaaaatccaa aaagacaaat tcgcctgatt tggttaatca ttatcggttt atttgcttct
    10261 cctgtcctca ttccactagc actggggttt atattggtta ttgggggact attggttgct
    10321 ttactagcgc tcatttttag tatctatgtt ttatgtatca gtatcttagg tgttggccta
    10381 ttcatgatca tctcaggaat cagtgtgttt ggacaatcga ttctgagtgg attattcttt
    10441 atcggtgtcg gtctgttcgc cacaggaagc gcagtattta tttcgccact gttgctgaaa
    10501 ggaacaaaag ggctttttta tgtcatgatg aactttgtta aatggatggg acgacgtttt
    10561 attacaaaaa gagatctgca ttcagcgggc gtttgaagga ggacacataa attgagattg
    10621 aaacattttt tgattaccgg tattggtctg atgcttactg gtggcctcct agcaggtgca
    10681 acttatacat taggcgctca aaagagtctg acttgggata atggtcctcg gttgatcgat
    10741 atggttactg agacaaaaaa aatcaaggat gcaaacaaaa tcgtcatcca tgcagaaaat
    10801 caaacagttt cggtcatacg cggattggat tttgagataa aaacgactta tgataagaca
    10861 gaaaagccag aaataaagga gaaaaatggc gtgttgactg tgaatgcagt caaaaaatca
    10921 acaggtgcaa tcattagttt agaagacaca agtgcacaaa tcactattgt tgtccctgca
    10981 gatcatgaat tatctgaatt gacaatcgaa gggaataata gtgcaattga tatcaacgaa
    11041 gtatcaatca aaaaagtgaa tactaagaca catgatggct gggtatcgtt caatgaattt
    11101 acaggaggaa caataactgc gaaagatgaa ttaggctcac ttcgattaga tgaggccaat
    11161 gtggataact tgatcgtcac aggtgaaaac agcaacttcc atctagaaga cagtttgatc
    11221 agcaaagaag gaaaagtcat cctaaacggt ggcaatttat ctttgcatga gatggaaaac
    11281 agtggctata ctatccaaac gtctggtgaa tcttatattg aaaaagacta ccagccaata
    11341 acaaatactt tgttagaaca aggacagaaa aagctaaaga tcactgctaa agacacatcg
    11401 atcaatatta cgacggatca agaggacaat gaaacagatt gatcctgtac tcaactggat
    11461 attttaagaa aaaacagcaa agatccttcc aatctttgct gtttttcatc gcttaacgca
    11521 cataactata gtaaaataag aaagaaaata tctaaaaaga aaacagggtg aaagagtata
    11581 tgaaggtaga agaaggtcaa agagaaaagt taaaaacaga aatggaaaga ttacatactt
    11641 atatcactca actaagccaa accttctatg atccagacaa agaaaaagtg atggttaatt
    11701 acccgaacaa ttcagaaggt cgccaattag agcaagtgta ccatgaagtt ttcaaacatt
    11761 tattgacggt gaagaaagag ctggattatt attctttacc gattatcgat acaggaatat
    11821 tgaagtatga tgggaagaag gaacgattta tctttaaatc tgttcgggaa gatttgcctc
    11881 tttcagcagg gatggatcta gaggtcttgg tagaagacta ttttacagaa gaaaagcact
    11941 gggttcgaac aaaattggat tatttaccgc aggcagccgg agggacacag gcaagcggtt
    12001 ggtacatcac tgaagacaaa gagttagaac tagaaggtgt aatggcgcgt attcgaaaaa
    12061 aaaattaaaa aagtaacatt agatatttat tgaaggataa aggagcgcag gaagtagcat
    12121 gagagaaaaa agagcatttt atatcaatgg ttatgcaggg attgtcggat tggtcgtatt
    12181 agctttagtt ggattgtttt tcttctattt aggtatgtgg caagcgaaag tttgggcact
    12241 ttttttaagt atttttcttt ggttgatcac gttattgttg ttaagttcgg caactgtagt
    12301 cagtcctaat caagcaaaag taattttgtt ttttggacaa tatttaggta cgattcgaga
    12361 aaatggcttt ttccttacga tacctttggc acaaaaaatg actgtttcgt tgaaagttcg
    12421 gaatttcaat agttctgttt taaaagtcaa tgatttggat ggaaacccca tcgaaatctc
    12481 agcagtagta gtctttaaag tcgttgatac agcaaaggca ttattcgatg tagcgtatta
    12541 tcaagatttt gtagaaatcc aaagtgagac agccattcgg catatcgcta gccaataccc
    12601 gtatgataca ttcaatgaga atgatttgac gcttcgagga aacacgacgg cagtgtctga
    12661 cgagttgcaa aaagagctgc aagagcgttt agcagtagca ggtgtcgaag taatcgaaac
    12721 acgcttgaac catttagcct atgcaacaga aatcgctagt gccatgctcc aacgtcaaca
    12781 agcaaaagcc attttgtctg ctcgacaaac cattgttgaa ggagctgtca caatcactca
    12841 aatggcctta gagcaaatcg aagatggaca agaaatcaat ttcaccgatg atcggaaagt
    12901 ccaattgatc aataatttac ttgtctcaat cattacagac aaaggaacgc aacctgttat
    12961 caatacaggc gacatgaaag accgtaagtg acatactttt tgtaagatag gaagaaggag
    13021 tttaggacgg attattgtct tgaactcttt ttttagttgt tttaaagaaa atgacaaaat
    13081 tgaaagaaga atgatagttg attcgaaggc agaattgttc gcttttttct ataatcagta
    13141 tataaagtaa gagagaaggg gaaaaagtga aaaaacttct gatcggtcta cttgttttca
    13201 tcgttgtggt acttgcaggg gtgaaaatcg cagattacgt agttatgggt ggagaagaat
    13261 actatgtaca aattacgaca gatggtacaa aaacagtttc aaaatcagac tctggacaag
    13321 aaacagtcaa ctatagatat atcctgcctg gctatacgaa agagggtaag gaaaaagaac
    13381 tggacttcaa gggacaattg actcgaccgc ttcgtaaagg agcatattta aaaatcacct
    13441 ggaacaaaaa taaaggtgtc acttcgtatg aagaagtcga aaaaaaagac gtgccgaaaa
    13501 cagcactgga aaaactaatt aaagggtagg gaaagaaatg aacaaaatag tcgaagtaca
    13561 gaatgtaaca aaagtatacg gaaaaaacaa tgagaaaaag acgcaagcat taagcggcat
    13621 cagcttcgat gtagaaaaag gcgaatttat tgggatcatg ggcgcttctg gttctggtaa
    13681 gtcgacactt ttgaatattt tatccacatt agataaacca acagatgggc atatccgaat
    13741 caatcaaaaa gatgtgacca cattaaaagg aaatcaacta gcagattttc gtgcgaatga
    13801 gattggcttt attttccaag atttcaattt attagaaagc ttgacagcac aagaaaatat
    13861 cgctgttcct ctttctttac aaggcgtcag accaaaagaa atcaagcaac gtgtccaaaa
    13921 aattgcagaa cgtttatcca tctctcatat tttggaaagc tatccttctg agatttccgg
    13981 tggacaaaaa caacgggtcg ccgccgcacg tgcattgatc acacaaccaa cgattctatt
    14041 aggggatgag cctacaggtg cgctggattc caaaagtgcc cgggatcttt tagatacgat
    14101 ggacgaattg aatacaaaag atcatgtatc gattcttctt gtgacgcatg atccgttttc
    14161 tgcaagttat tgccaacgta ttttattcat caaggatggc ggtatccatc aagaagtgaa
    14221 acgtggagat caaagccgtg attccttcta tcgagaaata ctatctattc ttggaaactt
    14281 ggaacagtag ggggagattg actatgcttt ggaaattatc acttactggg atcaagagcc
    14341 gtttaagaga ctacttggtg ttattctcag gattagttat ggcatctgct attttttaca
    14401 tgtttgaatc aatggcaagc aacgaagctt ttttgaaaag taatacgatc gttgcggcta
    14461 cagttatcat tttccgattc ggttctgttt tactagggat catcacattc gtttatattt
    14521 tatatgcaaa ttcctttttg atgacgatgc ggcaaaaaga ctatgcaatg tttatgatgc
    14581 tgggagcaaa aggcagaaaa atcgcacaga tgattttttc tgaaacattc ttagtaggga
    14641 tcgcagctac tttagtgggt tcagctgtcg gtgtaggatt gacaagtatc gtccaccgat
    14701 tattagtgga tcagttagat ctcaatattt cgcattttac accttttagt attcacgggt
    14761 tgttgatcac tgttttgttt tttgctgttt tgtttttatt agctgctgtt gtcaatgctt
    14821 tttcgattgt caaaaaaccg attttagcat tgattcgtgc ggatcagaca ccaacaagaa
    14881 tcaagcagca taaattcttg tttttccttg aagtagtact gggaattgct tttctaggct
    14941 gcggttacta tatgatgaaa cacgtgggga cctatcaagt ctttggtata ggggtagcct
    15001 tagttacgat cgtattagga acttatttca ttttccattc tgtgatcatt ttctttttat
    15061 cattactaaa gaaatcagaa cagatttcat tgaaaaaatt gaacaatttc acattgtcac
    15121 agttaaactt tcgtattcgt gaatatacac aaatgctctc aatggtcgct atgctgtttg
    15181 ccttggcact gggcgctttg acggtaggat taggttttaa aaatgaaatt gcagacttga
    15241 cagaaaacaa ttctggatat gatctagtga tgaataatcc tcgggcagaa gatcagcaaa
    15301 aaatcaaaga actttcacca acactttctg cttcttatac tcaaaaagaa gatgcgggaa
    15361 cgatttacta taatgcaagt gaatttgcgg ctcaaccatt gatcatgata aaacatgaag
    15421 atggaacacc gccgaagatc acgaaagaaa aagttcctgc taaaaaaatg aatgaactga
    15481 ctgtacaaga ggaacttcga agctatgaat tgccggaaca gcaagaaaaa gaaatcaagg
    15541 tagtcagtca atcagaattt gatcaactga acttgccaca atccacttta cagctagttc
    15601 aggtaaaaga cttccaagca aacctgaaac caatcgaaac acttgttgct caaaataaaa
    15661 tgaacaatcc atctttgcaa aaagtagagt acagcaacca gaaatatgaa atgtatgaag
    15721 tgtataatgg tctattctct ggttttgaat ttatgggatt cttcctaggt ttagctttct
    15781 tgaccatgct tgctagctgt ttgatgttca agatcttgtc aggaagcaag agtgacattg
    15841 ctcgttatga aatgcttgaa aagataggaa cacgacgcgg gttgcttaaa caatcgatcc
    15901 gtagagaagt aggcgtgtta ttcttagcac caggaatctt aggtgtagtc catgtcttat
    15961 tcggattaca aatgtttggt ctattcatgc aaaatccata caaagatatt tgggttccgt
    16021 ttacgatatt cttcgtattg tatttcatct actatgtact gacgacatgg ttatatactg
    16081 gaatcgtatt gaaaaagaga ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
    16141 aagcaaaaat agctcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
    16201 tttgaacacc gtttgttcgg aaaaagaggt tgtgacaata tttttgtcac aacctttttt
    16261 tcacggtctt ttattagaat atagtaaatt ttttctcgtt actcacggaa taagctgtta
    16321 agaaaacggt ttccatgtat actggaatca gtgcaaatga aagaaaagag ggtgcaagga
    16381 acatggaaat caatgaacaa aattttggcg aaaactatca cttgattacg atcacgaatc
    16441 aaaatgggac atctttgtct gtcagtgatc ttggggcacg ggttgttcat tttcaaacaa
    16501 taatcgataa tgaaaaacga gagttgatct taggttttga tgaagcaaaa gaatatctag
    16561 aaaaagaccc ttatatcggt gcttcaatcg gacgtatggc tggacggatt acgaatggca
    16621 cattcaaatt agatgatgaa acgttcgaag taaaaacaga cccggacact ggtcattgtt
    16681 tacatggtgg tgcacctagc tttgaagcca agcagtggca ttatacagtc atcaatggtg
    16741 aaaacgaagc atcggtcatt ttctatcaga ctagcccaga tatggaaaac ggatttcctg
    16801 gaactttaga tgtagaagta cggtatacgc tgacgaacga tgatacatgg cgagtgacgg
    16861 tccaagggat cagtgaccgc aaaacagtat tcaatcctac aaatcatgtc tatttcaatt
    16921 taaccggtga tccagcacag tcaatcgacg aacatatact ttggttgaat agccgattat
    16981 ttgctacgct caataaagat acaacgccta caggagaagt agtttcagta agtggcacag
    17041 catttgactt ccaacgtcct aaaaaattag gtgaggtctt tgccagtgat tttcgccaat
    17101 caatgatagt gggcggcatc gatcatccat ttttgttgaa aaaacaatct ggtttactaa
    17161 atgctgcgga attgacaagc ccagatgaaa aagtatcgat ccaagtaaaa acagatgctt
    17221 caggaatcgt gatttttaca gcaaattttg gagaagatgg accggaaatg cgcgggaata
    17281 aattagtaaa tcatggcggt atcacatttg agactcaaga actgccagga gcggagcgac
    17341 ttgaaagttt tggagatgtc actctggaac ctaaccaagt acgcgagttt gtaacggaat
    17401 acaaaataaa tataaagaaa tagaataaat taaaaaggga gtgtgacagt gtcacatccc
    17461 ctttttaatt tattctattt ctttaaaggc agatcaagcc agaatgattt gtctaaactt
    17521 aacaaggtta gtacaggaac agttgcagct ttaactgtac gagtagttgt ttctggtatc
    17581 tgtcttgccg taaaattgtt ccacaaatcc cagattgaag cgacacggaa gatggaagaa
    17641 gcgttgttga cgcgttttat cgttgaaaat tctaatcctg tgattggcac gccatctgtt
    17701 gtccatttga ttggcacatc gaatcgctta gacatattga ggatttcagc gtttgcttct
    17761 tcagttgttg ctgcttcttt tttcggttca tcatattttg tcaggtcgta cgcttcgatg
    17821 atcgcgttca atttttgtga atattggcga tctgtagcat aacgtccttc caaatatttg
    17881 gttgcttctt tgtagtcttt tgtttccgac ttccaagttg gtttgtagaa atcaggattg
    17941 ccagtcagtc catttttgat caagtcggca tagtcttcca aactttcctt gtaagaagga
    18001 tatttacgga attgtgcgcg gatttggtag ctttgtccgg aactatcttg ttcaagtgta
    18061 ttgaaggtca cgctgcttcc tttatatgat cctttgatac caaaaaggtt atgatttggt
    18121 tcgcttgata ataagctgtt tccagatcca ctttcaagga tagcttgagc catcatgact
    18181 gatgcgtaga ttccttcttt ttgcccgatt tcctgtgcat catctgcaat ctttttcaag
    18241 aactgatcag ttgtttggtt ttgactgaaa tggaacgagc catctaatcc atctaaaata
    18301 gatgagccgc ctgtcagcga acctgaagca gcaggtaaac ctgctaagtt tactcggaca
    18361 gcaaaatcag gcgtgaaatc tgaaatagaa gttactcgaa ctttatctcc tggttgaggc
    18421 gcatggataa attgtccgtt cccaatatag atacctacat ggtgagtaac accggaagaa
    18481 ggtccccaga agacgaggtc gccaggttgg gcctgactta ctggaatctg cgtacctgca
    18541 tattgctgtt cgcctgtcca gccaccgata taatgaccgg ttgctttcat atatacgtaa
    18601 taagtcaaac cagaacagtc aaaagtattt ggaccttttg ctccccatac atatggtttt
    18661 ccaagatgct tgaatgcttc tcgaacgatt gcttgttgga cattcgttgt atttgtaggc
    18721 aatacagcat caattggtga ttttacaaca aaatcagaag aagcagaaga tggattctct
    18781 ataccgcttt gatttgctgt atttggacga gatgaagcat tgttcgttcc gccattcgat
    18841 gaaccattgt ttggatggtt tggttctctc tgagtatttc cattgttatt cgatccagat
    18901 gggtttgttt gattcgaaga attcccgctg ttcgaattat tgttattatt gtttgaatta
    18961 tttgaaccat tattattgtt gttattaccc acatctggtt tgtttggcgt ttccggatct
    19021 ggtgtactag ggacttcttc actaggatca gttggtttct ctgttgaagt atccggtcca
    19081 gctttaaatt ctgcagtaat agataaattg ttcgtaccta ctgtaaaggt ctttgcatca
    19141 taatttactt ctttggcacc attaacagcg actgaagcaa tttgttcacc ttcagctggc
    19201 gtaatagtgt aagttacctt cgtttccgct aataaacctt ctaccgttcc actttccgta
    19261 atatccaaag gttgctgttc aggggaatcc gctgtcgaag cagtcagttg gatcttacca
    19321 atcaaagaag gagcaattgt aagggaatat gtctcggctg gcttttcatt ttctgatgga
    19381 tcagccggat caatttcagt tggctcggtc gatcctgttg ttgagttagt tgttccagtt
    19441 gggtcttctg taaaagaatc cgttgtttca ctagtagctg agctggatgt agtcgtatct
    19501 gttgtgactg tttgatcagc tatttgatta tctacggcaa atacggtatt ggtcgacaaa
    19561 acagacggag caatcagcca agtagcagct agcaaacgta tcgttttttt atttttcata
    19621 ataaactagt ccttttcctt tcattgattt tctgtcttat tcagcattca ttagacattt
    19681 tttggaacag ataatcgtgt cgaaaagctt tttcttttca taaatctgtc tgtgtattct
    19741 aaataaaaca atttgtaaat aagcaacgag cattcattcg tttttctttg acgttcattt
    19801 tattgcatag accacctttg tttggggata aacaagtggg gataaaaatg gttatgcata
    19861 tgtaaattat accaaagttt ggaaagtatc acaaaaattt ataaaaataa tgatcctaaa
    19921 aagaaaaaga ctgaaatatg tgataaacat ctcatacttc agtctttttc attaactatt
    19981 cataagacat taggtcttca tcaaacccgc taacgagagt agtgatcatt gctacgacta
    20041 gactaacagc cagtcctgct aagtaccaga aaaaagtacc ggaaatgaat gcaggcaatg
    20101 tggtcagact accaaataca aaagcgatcg ctttcacttg ggcaaatccc attacagctc
    20161 caccagcagc tgcaccaatg caagcagcaa caaatggttt tttcaatggg atcgcaattc
    20221 cgtaaacagt tggttcggtc actcccgcaa gtaaaatcga agcaaaagca gaaccggaga
    20281 tcgaacgggt ttttgaacgt ttacttcgca gaaatacacc taaagctgca ccagccattc
    20341 caaaattggc tgctcctgtc aacggaacga ttcggtcgaa cccataagca gagatattac
    20401 tgatcatgac agggtttaca gcccattgga taccaaatat taccaaaata ttccaaccac
    20461 cagcgatgat tgcgccactg atcggtccgt taatatccat catccaagta attgcggcag
    20521 ctaaaccttc gccaaggtaa acacccgcag gcccgaatac cattaacgtc aaaggaaccg
    20581 tgataactaa tgaaagtaaa ggtacaaata ctaattgcat attttcgcga atatattttt
    20641 tgaggaattt ttcaacaaaa gaataaaaga aaatacctaa aatcgccggc atgacagtag
    20701 acgtatactg catcagtact acaggaatcc ccagaaaatc agtagctgct ttgccactgt
    20761 ctgaaagtaa agaggtcaga ttcggttcaa tcagtgctgc agcaatgaca gccgcaatat
    20821 atccattcac tttaaacgtt ttggcagcag aaaaagcaag aaagattggc agaaaataaa
    20881 agacggcatt cgacgcagca gacaaaatat gataagttcc gcttgtttca ctaagccagc
    20941 caatcgtagt cgctaaaaca accagacctt ttaatatacc agaaccggca aacagcggaa
    21001 tgatcggcgt aaagatttcc gatatcaaac caaatagttt ttcgatcgga tttagctttt
    21061 ctttcggagc tgaatgatcg atttgacgcg tcaattcctt gtaagcagga gccaatccct
    21121 gatccatgat tatttctgca tatctatcga gtaaaatgaa atacttaatg gaagggattt
    21181 gctgttttaa tttttctgca ttggctactg gctgtttttt caatttgata cgaagccgag
    21241 tcgcacatgg aaggacagaa tcaatattgt cttttcctcc caactgttca atcagcatat
    21301 gaatctgctc ggacagtacc atctgttcag gattcattgt cgtttccatt agaagaccac
    21361 tccactcact aatataatat tggcgtaagg tgtcatttcg cctgtacgaa taaatgcatg
    21421 acagttattc aactcttttt tcatttcttc atgtgggata aagctaatcg gtgtatctgg
    21481 taacaataat ttgatttttt ccaatatttc cgggttcatt gtctttattt cttccgccaa
    21541 ttcaatcgat tgtacctcaa gttcttctaa aacgttttct aaaacagata aaaaacttgg
    21601 aagaccgttt gagacagcca aatctatttt ttcagtacca aaggggacag gcatgcctgc
    21661 atccccgata cttaatttat caaagtgccc catttgtgcg attactcgtg aaatatctga
    21721 attgattact tttccttttt tcatgtttgt ttcttcctct cattgttaat tttagactgc
    21781 tgattgttct aaagcttcgc ggtaagggat agaaggttgc gcaccataag actgcacagc
    21841 aacagaagag gctagatttc cataaaggat cgcttctttc aagttactaa ggtctggctg
    21901 aagtgtgctg cttaatgcgc caataaatgt atcacctgcg gctgttgtgt caacggcatc
    21961 cactttaaaa gcagggacga taccgtgttc tttttctgta tgatagaaag cgccggcact
    22021 gcctagtgta atgattactg ttccaatacc taattcatgg agtttttcgg ctgctgcgac
    22081 caaactgtta tgatctgtca ctctgattcc tgtaatgatt tctgtttctg tttcatttgg
    22141 tataatgata tctgtctttt caagtagttc agtaggaatc gtttcacgag ctggtgcagg
    22201 attcaggatc gttgtttttc ctgcgtcttt tgcaatagaa aaagctagga tcgtcgcatc
    22261 taagctactt tcaaactgag caataataaa gtcactgttt gtaatcagtt gcttgttttt
    22321 taggacttct tgttcatgga aagcattatt tgcccctgca tggatcaaga tactattttc
    22381 tcctgctgcg tctaccataa tcattgcttg tccggttgtg gcgccttgga tttcagctat
    22441 cccagatgta tcgatgtttt cttttgtcag tagatctaat aactgttgtc cttcgctgtc
    22501 agccccaacg cctccaataa aagatgtccg tgcgcctgaa cgtttggctg ccactgcttg
    22561 gttggcacct ttacctccgc cagcgtgaaa aatctcatgc gcatgcatcg tttcgcctgg
    22621 cttgggcatg ttggttaaac gaagggtagt gtccatatta atacttccaa ttactgtaac
    22681 cgtgttcatt ttacaaacct ccaactatct catttatttt gatgggaaaa ttatttttta
    22741 aaacgtttta gtaaaacgtt ttaatagttc gataaaaata tagcacacct tgaaagctat
    22801 ttcaagggtg cagttgaaaa tcttttttct aatcgtacag gtaatctttt ttcttcccat
    22861 tctttttctg ggaattggat ccgatctaac aataacttgg cagatgtttg tcccaattcg
    22921 aagattggtt gtgcgatcgt tgtcagtttc ggcttgatat actcgcacat atctatatta
    22981 tcatagccaa taatgctgta gtcttcaggg atgctttttc cagcttcctc taatccgcga
    23041 tataatccaa aagctaattc atcatttaaa gcaaacacac cagttgcagc agaatcaaga
    23101 agttctgctg tgatctggta gccaccttgt tttgagaact gtgtaggtaa gaggatcagt
    23161 tggtcgtgag ggatttggta aacatcaaga gcagatttaa acccctcaat ccgagcgtgg
    23221 acattttccg gtggattttc aggatagaca agggcgatcg tttgatgtcc tagactaagc
    23281 aagtgcatgc ccgctaggta accaccgcga aaatcgtctg ttcgtacggc atcactaaat
    23341 ccttcagatt ttttctgatc caaaacaatg aagggccggc cttgtttttt caaattttcg
    23401 ttgattgcat ctgtagagac agcagaagtc gcgataatga atccatccac acctcgacgt
    23461 gtgagctcgg ctagatattc gatttctttt tgatgatcag aatcagcatt acataatatc
    23521 gtgacgaaat tctgcttata caagatatcc tcaattcctc gcatcagtgt actgaagaat
    23581 ggattcgtga tatcaggaac gagtacacca attgttttcg tttccttggt aatcatttgt
    23641 ctagcaaaat agtcaggctg atagcctaat tcttctttgg cagctaaaac tttctgaaca
    23701 gttttcggac tgaatttctc cgtatttcca tttaatatca aggagacagt tgtgattgaa
    23761 acgccggaaa gtttagcgac ttctttgatt gtgatctttt ttttcaccat gttcatactc
    23821 ctatctaaat cctgtggtta ttttcccatg aaatcaaaaa gaagtaaatg aaaatcacgt
    23881 gtgccagtac ttatttgctt tgttggatag cttcgattat ttgtttcgtt gagcgtactc
    23941 gtgcagtttt cggaaatatc ttttttatga acaattgatg ggattctttg tcacgatcac
    24001 tgcaagcatc ttcagcaagc acgatatggt acccgtgttg aaaggcatct aatgcagtag
    24061 catatacacc gtttgttgtc gctacaccgc ttaaaatgat agtttcgatt ccacgccggc
    24121 gaagttgaag atcaagagaa gtaccaaaaa aagcacttgg attgtatttt gtgatagtca
    24181 acatattatc tgtatctttc aaagaatcct tcaaaagtaa atcagtgaat tctggaggta
    24241 cagaagcagt cattccaccc atatcagttt gttgactcaa ataagggaaa gtggagatgt
    24301 ctacgttcac taaaactttc aaggcagcgg tacgtttcaa tgttcttact aacaaatcat
    24361 tgtttgtcag aatagatgct gcagtgtgtg gagagagtgt accgttattt acgatctctt
    24421 tttgcatgtc gatcgcgaca aaagcagttt ttgataaatc aaaaatcata ttttccttta
    24481 aatcattcat gatgttcctt ctttcaaata acaatgtttt ttatgtaagt aaatcataaa
    24541 tcgtttattt gtcaactaaa ttaaaggatt cctttcttta gtctagagaa attcagtggt
    24601 aaaataaata agtgtgagaa aacgcattcg atgcatagat atagcgagaa atatcgtgaa
    24661 aatggaggaa tcagagtgaa gtatgtcgaa gtaaaaaatg aatacaaacg atacaaaatg
    24721 ggagaaacga ccattacagc aaatgacgat atttcttttt caattgaaaa aggcgaatta
    24781 gtgattatac ttggtccaag tggagcagga aagtcaacga ttcttaatat tttaggtggg
    24841 atggatacac ctgatgaagg tcagatcatt attgatgaca cagatattgc gcaattttca
    24901 gataaacagc ttactgctta ccgacgaaca gatgtagggt tcgttttcca attctacaat
    24961 ctcgttccta atctaacagc aaaagaaaat gtggaattgg caacggaagt ttctcctgat
    25021 gcgcttgatc ctgtagaggt attgaggcaa gtgggcctag ctcatcgatt gaataacttt
    25081 ccgtcccaat tatcaggagg cgaacagcag agggtttcaa ttgctcgtgc actagctaaa
    25141 aatcccaaac ttcttttatg tgatgaaccg acaggcgcgc tggattttga aacgggcaag
    25201 caagtattga aattattgca aaacgctagc agacaacacg gcaataccgt tttgatcatt
    25261 acacataatt ctgctttagc gcccatcgct gatagagtca ttcacattaa tgatgctaaa
    25321 gtaaggtctg ttgaattaaa tgatcatcct tcatccattg atgagattgt atggtagggg
    25381 gattttatga aaacaagaac atatcttaaa gcaggttttc gcgaaattcg gcaatctaaa
    25441 ggacgattca ttgcaatcat ccttattgtc atgctaggaa cacttttatt tgtaggtgtc
    25501 aaaactgctg gacctgtcat gcaaaaaacg atggatcact atgttggtac agcaggactt
    25561 tctgatttgc aaatcgtttc tactggtggc ttgacacaaa aagatattgc tcaagcagaa
    25621 aagatcccag atgcaaaagt agaaacagga aaacagatct attattccaa tactggcaaa
    25681 aacgaagtga ttcaaatatt ttcttataac aaaaataaca agcagaacaa gctacaatta
    25741 actgatggaa aactcccgga aaaaccaaac cagcttgtat tggatgaaaa agcggaagat
    25801 gaagggtata aaatcggtga tatttatcag attgattcag atgaattgaa agaaaaagag
    25861 tataccatca cgggctttgt ccgttctcca ttgttcatca acaatttgga gagaggatac
    25921 gcgaacgtcg gaaatggtag cgtcgattat tttgtctatt tacctgaatc cgtttttaaa
    25981 acagatattt attctgtctt atatcttgat tttacaaacg taaagaattt gaatacgtac
    26041 agcaaagctt acaaagataa aatggagcaa aatcaagaaa aggcagaaaa gtatttacaa
    26101 gatcgacccg aagaacgctt agaagagctg aaaaaatcgg ccaacgaaga attagaaccg
    26161 gcacagcaaa aaatagcgga tggcaaagca caaacaacgc aagcaagaac acggttagaa
    26221 gatgctaaaa aacagttagc gcaacagtca gcaactatcc agcagcttcc tgaagcgcaa
    26281 cgaacaatcg ctgttcaaac attatctcaa caagaagctc aacttgctga acaagaaagg
    26341 caattagttg agaaagaacg tgaactggcc tctgcagagg aagaattgaa tcgaaatgca
    26401 gaaaaaattg aaaatttaac taaaccatca tacctataca atgaacgttc agaaaatgtc
    26461 ggttttcaag aatttggtga tctgtctgat cgtatcgctg cgattgccaa tgtttttcct
    26521 gttttctttt tctttattgc cgcattgatc acctttacga caatgacacg tatggtagaa
    26581 gaaaaccgca gggaaatcgg gacattaaag gcgttaggtt atacgaaagt tgaaatcgca
    26641 aaaaaatatg caatctatgc aagtctagcc tcagggatag gtattgtttt aggaacgata
    26701 ttaggaacga atcttttgcc aaggatcatt tttgagctgt ctaacgagcg gtatgatatt
    26761 gggagtgcgc ttatttttta tgactggccg ccgattttac aagcagctgc cgcattcttc
    26821 attgccgcat ttggcgctgc aatgatcgtt ttattcaaag atttaagaga acggcctgct
    26881 gcattgcttc aaccgaaagc accgaagcca gggaaacgca tttttcttga atacatcact
    26941 ccgctgtggt cacgccttag ctttaatcaa aaaatcagtt accgaaatct cttccgatat
    27001 aagtcaagaa tgcttatggc gattatcgga attgctggat gtgccgggct gatggttgca
    27061 ggcgtgggat tgaaagattc tctaagttct gtttcagcca aacaatttgg accgatcatt
    27121 gactatcaag caatcgttac attggattca tccacggaag atagtcgtca aaaagcagcg
    27181 gatatgctgc agaagcaaga aaaagtaaat gagatcctag ctattcatac tcaaaccgta
    27241 gaattgagaa aaaaaggaca tgcttcgcaa agtctgacag tgatcgtacc aagtaaaaca
    27301 gctgatttgg atcgttttat ccatttaact gatcttgaag agaacccacg ctccttgcct
    27361 aaaacaggaa tcgctattac gcaaaaagta gcagaactat ttgacttatc tacaggagat
    27421 catatttcgc tttatgatga agatcaaaaa gaatggaaag tcgaagtaaa agaaatcgtg
    27481 aaaaactatc taggtagctt tatttatatg tcccctgaat attatgcttc agttactgat
    27541 caatcgatga tggataatgc atttttgctg cgtacagata agatgacaac aaaagaagaa
    27601 gaaatgcttt ccgagaaact gcttgccaca gacgctgtga caaatacgtc tttcatgtcc
    27661 aaacaaatcg aaacgcaaga agaatcaatg gcgaacttag actcggttgt cttaatcttt
    27721 gtcgtattat caggactgct ggcttttatc gtcttgtata atctaactaa tatcaatatt
    27781 tccgagcgtg tacgcgaatt atctacaata aaagtccttg gattttttga taaagaagtc
    27841 acgatgtaca ttgttcgaga aaatatcatc tttactcttt taggaatcat cggaggattt
    27901 ggtgtaggat atatattgac tgacttcatc ttgcaacaag cgtctatgga gaatgtcatc
    27961 tttccattag tgatcacatg gtgggcttat gtcttatcag caggattaac tatcgtattt
    28021 acggttattg tgatgattgt tacccatttc aaattaaagc atatcgatat gattgatgcg
    28081 ttgaaatcga atgaataaaa acagaggttg tgaaatcagc ggtttgacct gagtattaga
    28141 gaaacaaacg gcaaaatagt ttttcatatt ttgccgtttt ttgagctaat accgaaggac
    28201 agctgattga acaccatgaa tcagaggttg taaaaaagcc gtcctgcatc gagcaaaaag
    28261 aatactcaaa taaaattagc tcctcatgtt ttacatttga gaagactgat tgccgtagat
    28321 aaaagctttt gaacaccatt tatagaagaa acaagaatat tttgctattt taaacaagag
    28381 gatagacaaa tacatgtctg atctcttgtt ttttattggg caacagacct cttagaatgt
    28441 ataaagataa attttacaaa cgctttcggt tcagttataa taataaatga taacttgtat
    28501 atataagttg tggtaggtaa taaatcagtt gaatggaggt aaatatccat gacatttaaa
    28561 gacaaagtca tttaccaaat ctatcccaaa tcttacaaag acagtaatgg tgatggaatt
    28621 ggagatttaa aaggaatcaa agaaaaactc ccgtatctga atgatctagg catcgacatg
    28681 atttggctaa accctattta tccaagccct caaaaagaca acggttatga tatctctgat
    28741 tacacagcca tcgatcctat ctttggtacg atggaggaat tcgaacagct aatagccgaa
    28801 gcaaagaaat accggataga aatcatgctc gatatggtgc tgaatcacgt ttcgacagaa
    28861 catgaatggt tcaaaaaagc actagcaggg gaccagcatt atcaggactt cttcatttta
    28921 agagaagagc caacggattg ggtatcaaaa tttggtggaa gtgcttggtc gccatttgga
    28981 gatacgggga aatattattt gcacttatat gatcggacac aagctgactt gaactggcgg
    29041 aaccaagcag taagagagga actattcaaa gttgttcgtt tttggatgga aaaaggggta
    29101 aaaggattcc gtttcgatgt tatcaatgtt atcggtaaag acgaacaatt gaaaaataat
    29161 aaagaaaatg atggcaaacc tgaatatacg gacaaaccaa tcacccatac gtatctaaaa
    29221 gaattgaacc aagcaacgtt tgggacggat tccactatca tcacagtcgg tgaaatgagc
    29281 tctacgacga tcgaaaattg tatcttgtat acagaaccta gtagagaaga attatcaatg
    29341 gtctttaatt tccatcattt gaaagtcgat tatgaaaatg ggaataaatg gtctactcct
    29401 ccatttgatt ttgcagaatt aaagcgacta tttcatacat ggggagaaaa gatgagtgag
    29461 ggtaacggct ggaatgctct attttttaat aatcatgatc agccacgtgc gctcaatcga
    29521 tttgtagcta tcgatcagta tcgaaaacaa ggagcagaga tgctagcaac catgatccat
    29581 ttgaaccgtg ggacgcccta tatttatatg ggagaagaaa tcggcatgat cgacccagac
    29641 tattcatcga tttccgacta tattgacgta gaaagtttga atgcttataa gttactcctt
    29701 gaacaaggat tcacagaaaa agaagcgttt cgtcggatca aagcaaaatc tcgcgataac
    29761 tctcgaacac ccatgcaatg gtcagacaca gaacaagcag gatttactac agggaaacct
    29821 tggttaaaag ttggaggcaa gctagaagag attaatgtag aaaaagaact atcttctgaa
    29881 gattctattt tgtcctatta caagaaattg attcggctga gaaaaacata tccaatcgtt
    29941 gcacaaggag attatcatgc ttatgaagca aatcatccgg aagtttatgg ctttcttcgc
    30001 caatttgaag gacagcagct gcttgtatta acaaatttct atgataaaga tacaaggatc
    30061 gccatcccag aaggattcac agaagccaat attcttatcg ataattattc cgatactgtt
    30121 cttcatcaaa gtctggagct aaaaccatac caggcaattg ctttgctgaa aaattaaaac
    30181 aaaaacacaa aacgtcgttc ctacgagcta ttctcgtaag atcgacgttt tgtgttttat
    30241 ttaaaataat cttcttcaga aaatgaagag ttttcatact ctcctttatc gccataaaat
    30301 ttattgtagc gttgtttgaa tttacggaaa ataaaagtaa cgagtaattt gactaacgta
    30361 tagataggaa taccaaaaat aacgcccaca attcccatta gatcacccat gacaagtaaa
    30421 acgattaaga tcgtaatcgg atggatctgt aaacgatcac ccattactcg aggaatcacc
    30481 aagtcaccgt ggagaagttg tacggcaaac cagacaatga caaatttgac agccataaaa
    30541 gtagagtctt ggaatgcaat gatcaaacct ggaaagaagg cgataaacgg cccaatatat
    30601 ggaatgatac ataacaaccc agcagaaata gctaaaacac tagcaaattc taatccgatc
    30661 agtaagaaac aaacccagta gactgctcca aggataaatg aagcaatgat ttgccccttt
    30721 agaaatgaac ccaattgttg attagcaatc tttgtcaaat ttttaaaatc tgtacgaaat
    30781 gctggtggga caatcgctaa aactgactga taaaattttt gaggatctct caaaaggaaa
    30841 aaagcgacaa ttggaccagt aaataaagta ataaaaatag ctgtaacagt tgaaaaaatg
    30901 ttgctgatcc gttgtgcgcc actttcccaa taatttccaa taaatccagc tatatcagac
    30961 gtcacgtcgt caatagaagc gaaaaattga ttaaatgaat cggcaaaagg tgttttctcc
    31021 aaaaattgtc ggagactctc ttgaaaatct tgaaacaaat ctggcaaact actgatcaat
    31081 gtctttgcct gttcttcaag aaaaggaata agccatatgc ttccaagccc caataaaaat
    31141 aaaataatca cataaacgat aatgactgac aagttcctcg aaaggtgctt gctcaaacgt
    31201 ttgatgactg gttccaaaag ataatacaag atcagtgcaa acaataatgg gggcaagata
    31261 gtagcgataa tgattccgac aggcttgaat ataaacgaga tctgatggaa aaaataaatg
    31321 atcagcgcaa gtaagataac tattcctaaa acatagtaag aggtttttcc tcctaaaaag
    31381 cggatcaaac gattcgttgt atctgtttct tttttcactc tttttcctcc tttatctata
    31441 aattcattat agacaaaagc gggtataaaa ggaaaatgat agaggttgat tgcaaaaata
    31501 tcgtcaatca ttcatataaa ctttctcatc aattaaaata agaaaacgct tgacagttct
    31561 tttaaccata tgataaatta aatcctgttg ctaaaacgta actattacgg tttgcgaaaa
    31621 gcgaatcgga acattcaaat gaaagaagat gagaagagga gtgtcataaa tgaaacgatt
    31681 gataggtata ttggccatgc tggttttagc aggtatgtta actgcttgcg gggcaagtgg
    31741 gaaagccgag gacagtaaag aaaaattgag cgtgatgacg accttttatc ccatgtatga
    31801 ttttacaaaa gcaatcgtcg gggatgaagg agaagtagag ttattgattc cagcagggac
    31861 tgattcccac gattatgaac cttctgctaa agacatggca aagatccaag atacagatat
    31921 ctttgtttat aatgatgaaa acatggaaac atgggtcccc gcaatccaaa aaacattaca
    31981 agaaggcaat gtccatacga taaaagcgac agaaggtatg cttttacttc cgggaagtga
    32041 agaaggacat gatcatgacc atgaacacgg ggaagaaggc catacacatg agctagaccc
    32101 acatgtatgg ctagctccaa gtttagcaat caagcaggta gcaaacatac gtgatcagct
    32161 gattgaagct tacccagaaa aacaggaagt atggacaaaa aatgcagcag cctataccga
    32221 gaaacttcaa gcactccatc agctttatca agaaaccttt aagcaagcaa aacaaagatc
    32281 ttttgtcacg caacatgctg cattcaacta tctagcgtta gaatatggat tgaatcaagt
    32341 atcgattgct ggtctttctt ctagtgaaga accgtctgcg gcgagaattg ctgaactgaa
    32401 acactttgtt aaagaacatg gaatcaacta tatttatttt gaagaaaacg ccaaagatag
    32461 tattgccaga acgttagcaa acgaagcagg tgtatcttta gaagtattga atcctttgga
    32521 aggattgacg aatgaacaaa tagaaaatgg agaaaactat ctttctatca tggaagcaaa
    32581 cctcgaagcg ttgaaaaaaa cgacagagac agaaaacccg ctagaagata ctttgacccc
    32641 caaaaaagaa aagagcgtct acaacggtta ttttgaagac gaggatgtac aagatcgctc
    32701 tctttcagat tgggcaggaa cttggcaaac agtagatacg tatgtagaag atgggacttt
    32761 tgatcctgtt tttgaatata aagaaaaatt gaatcaggat aagacagcac aagaatataa
    32821 agaatattat acgaagggct atcaaacaga tattgcttca atcaagatca gtgagaaaga
    32881 gatggcattt acgtttaaga atggaacaac gaaacgttca aattatcgtt atgtagggaa
    32941 agaaatcttg acctacgagg caggaaaccg tggcgtaagg tatctattcg aagcagaaga
    33001 taaggaaagt ggcgctttta gatatattca attcagtgat cacacgattg caccggctga
    33061 ctccgaacat tttcatttat atgcaggaaa tgaaagtcaa gaagtattgc tgaaagaaat
    33121 ggagaattgg ccaacatttt atccagatac aatggatgga cgtgaaattg cacaggaaat
    33181 gcttttgcac tgataggcaa aaaagaaccg aagggtttgg atcggctggt catttagtcg
    33241 gtccaaacta ttcggttctt tttatgaaat caatttcttt aatccacgga tagaggctgg
    33301 gatggtctcg ccgtttgcca acgtgacttc tctggttttt ttattaatcg accgcacatt
    33361 ttgtgtattg ataacatagg atttatgaca acggataaaa ttccgatgca ttttctcgat
    33421 ttctttgatc ttctcataga attgtaattg ggcatgagtc aaatggactt ccagtttatg
    33481 tggcgctcct agcgaagtag aaaaatattg gatgtctttg atcggtaaaa atttgatctc
    33541 atcattaaac tctattttga agatatcttt ttggatttgg gacatttgaa aatcttcgaa
    33601 aattttgctc aggatatgct cgacattttc ttttaaatca taaagatctt ctttgacgat
    33661 ataatcagtc ggttcgatgt tgctttttaa gattttcatc gacatttccg tatgggaagt
    33721 cacatagatt atttttccta aaggatctaa ttcacggatc aatttaccga tcgctacacc
    33781 atccatttca ctgtcttcta gttcgatatc taaaaagtaa ataccaaaag aagaagttct
    33841 gatcttggct gcattgagaa gttcttccgg atcagaggta gaaatatgga cataactgtc
    33901 taagttttca atcatgattc tatttttgat caccgttgat aaaacttcta attgttttgg
    33961 atcgtcttca caaatataaa tgggaatcat atcattactc ctttatttta tttgtatata
    34021 aatcgtttga gaaaaacgct ggttttcaat tgatgtttca aggaaaagat aaggattggt
    34081 ctttgtcaat tcatccaaga taaacaagcc aaggccttcg ttttttggat ttgtttttgt
    34141 tgagaaccct ttttgtttca actggtttaa cggtaatgta tttgttgttg tattgatcac
    34201 acgaatcatc acggaatgat tcttcttgag tatggtcaat tcgatttttt tcttctttgc
    34261 agcactagct ccttcaatcg cattgtccaa gataatacca agcatacgta caaggtttac
    34321 cgtatgtttc gtatccagtt cgattggctc aggaaaatga agctggacga tcaacccttt
    34381 ttcctgtgcc atcatgagtt ttaatgacag taaactacgg atctcaggaa ctttcatatt
    34441 atccaacata cttagattgg ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt
    34501 ttgatcataa taagaccgta atccttctaa gtcgccttct gaaatatagc tgtgaagaga
    34561 aaaaagcaga ttttggtaat catgtttgaa ttctctcaag tcttgatagt ttttttctag
    34621 atctttgata taatcctgtt gcatcctgac ttgttgctct aataactgat cagctaattt
    34681 cttattagag attttccaat aataaaccaa gatgatcagg ccaacacaca gatatagaat
    34741 gaaaaaactc gtattcaaag ctaagacatc aggtgtttca ccagaaaaac gggtataaaa
    34801 aataatgata tagtatgtaa ataatatcaa tgtcccgatc gtaccgatca tacggcggtc
    34861 cacagtgtga aaccaagtct ctaacgattt ttttaaacaa aaagctaata aagcagataa
    34921 aaaagcggat aagacaatgt gaaaatactg caatgaatct cctggttcaa taaccgaaga
    34981 tttcaaaaca aaaaaatcgc cgatgcttgt taagtgatct cctaaaagcg acgtcaataa
    35041 cccaaaagga agcgcgtaaa ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag
    35101 catcagcatt cctatagcaa ttagagcagt aaaaatccct aaaatcgaat agacccatcc
    35161 aatcaaaata caagaaatca gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg
    35221 gtcaatcaat aagatacaaa ttgtgtaaat caaatattgc aacgctatgg accataaaaa
    35281 cataaagtac agctcccatt gatttttacc aaattatagc acaattggcg tattattttt
    35341 ttaatttttc agggattttg aattcagaac tcataccaaa gcagcagcca gatacaaaaa
    35401 agtcagataa gttagttaat tgtttaatga ttgttttttt catgaaagat tcctccaaaa
    35461 ttcatcaagt agtttgtaat aggaagcaac gtagctattt ctaaaacaat cccaagtagc
    35521 aaataggtag aaaagtaaga agctgagaaa aataacgtaa tgagagaaaa aattagaaac
    35581 atgctgatcg ccttctttaa taatggggga cgtttctcgg gttcaatggg attgattgct
    35641 gtacctcttg atccatagcg aagcagtaac aaaaagcagc tgattaacaa aaagaccatc
    35701 attgggaagg agatagtcaa ttgcagatgt ttcatcacca agggaacgcc ccataaataa
    35761 agtagtgtcc acagcagaca aacgaattca gagtcggaat gcaatccata agcatatctg
    35821 cggatgcaga tataagaaag aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga
    35881 gaaaagacta gaacgaccgc tagttttcca agattattca ataaaacttc taagacaaat
    35941 tttacttgga catacaccat atcttcttcc gatttgtctc cgctgaataa ggcttctaat
    36001 aatttgttca caactttatc tttcatctca tacgtacctc caccacaaat tttatgctaa
    36061 aaaaaataaa aaacgtcttt tgtttcataa aaagtggttt tgacatcaaa accgtcatga
    36121 tctcctaaaa aaatcttcgt tttccaagaa ccttaccaaa aaaagtgaca gttttgatac
    36181 gaaaagtact ttttatgaca agtttgtgct ttttcgagtg atttatgtta aaaaattact
    36241 gaccttataa aaggtgatgg agggattctt atggaaaatg aacaattgtt tgaagtcggc
    36301 gaaacatatg catttaaatc tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa
    36361 atctatgaaa atagtgtgat gatacgtgtc atgaaatgcg cagtagaaga caatgatacc
    36421 gctcataatc tgcactggca gttagttgta cgcaaatcaa gtacaataga agaataaaaa
    36481 cagataaaat gttgagagca taaagaggtt gtgagtctcc cgtttagctc tgagttacaa
    36541 ggaataatgt taaaaaacag cttttcatgt tttatgaata ttgccttgtg accgaggtgt
    36601 tggcaggaga atactgttta tttgaaaaaa gggctgcgac aagacttttg tcacagcctt
    36661 tattttcatg aataggagtt acttttggct acttttagag aagtagcttt taaggagcag
    36721 ataaagtcaa ggcttttatt tctataaatg aagtagtaaa atgaagaaca gattttatta
    36781 caggaaggat gccgataatt gtgatcattg ttgcaggaac gatcggtgca gggaaaagca
    36841 cactaactga aatgcttgcc caagatttag aaacaaaacc tttttatgag aacgtggaag
    36901 ataatgaagt attgccgcta ttttattcta atccagaaaa gtatactttt ttattacaga
    36961 ttttcttttt gaacaaacgc tttttggcaa tcaaagatgc tttcagccat gatgacaacg
    37021 tactagaccg ctccatctac gaagatagta tgctgtttca tttgaatgca gatttagggc
    37081 gtgtttctga agtagaagta aagcagtatg aaggtctttt agaaacgatg ctgaaggagt
    37141 tagaagaaat ttcgccacaa aagaaaccag atctactggt ttatattcgt gtatcctttg
    37201 aaactatgtt agcgagaatc aaaaaaagag gccgggaata tgaacagcta gaacaagatc
    37261 ctgaactcta ttcctactat aaagaactca atcgacgata cgaagagtgg tatgaacagt
    37321 ttgatatatg tcccaaaata gtgatcgatg gggacaaata tgatttcgta gccgatccgg
    37381 catgtggaga acaaatcgta caagaaatca agatgcgggc aaagaaaatg acagaagagg
    37441 cagatgccca gtcgatccgc tacactacaa ataacccgaa agcaaaggga atctttacaa
    37501 ctaaataata taaaaactaa gcaaattgct cactcgcaga tatttctctg tgagcagcat
    37561 cttgcttagt ttttttgatt cattgagatc aaatagtgaa ccgattcgta tgataaaatt
    37621 gccataaatg aaatgtctga gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa
    37681 tcgaatgggc gccaaacaga tgatgacaga aaacaaggac aatagctcct gcgataaagg
    37741 caagaacgac acttagatag tggaaaaatc gatcaaaatc tgctttttct ttagaaatac
    37801 aataatcata taagttcgtt gctacccctt ttaaatttcc agttgtaaat aaattagtgt
    37861 aggcacgccc gttgatttta tcaaaagcta cccactggat cgatgcagta aaagcaatca
    37921 aaatcgttac taatgcggga tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca
    37981 ctgcttcata atatagaaca aacaagcgcc agaaatgttt ttcttttttt ctaaaataat
    38041 caattagata ctttgttaga agaatcccta agaaaaagaa aagggtagac aatacttttt
    38101 taccaacact tagccattct ccgtcaaatg cttggatgat cgatagaatg atattgccag
    38161 tttgagcaga ggcaaaagca ccatattgga tataagtata actgtccatg gctcctccga
    38221 taaatgtcag caaaagtcct attgtacgcc cctcgtgaaa ggaagtctgc atttttttca
    38281 tcacctttta tgaatatttt tgtcttcctt atcttagcat aaacgattga aaaaaaggaa
    38341 aaattttcag aaaactaaaa tcatatcgct tcccattttc atttttagaa aggtctgtta
    38401 gtataaagaa aaaatagaca acgaaacttt aaataaccaa cttgaaataa acaaattcaa
    38461 agaagaaaga tgggaaggaa atcaatcagt tgaatgaaaa agaaattctc cgattacaaa
    38521 aagaattgaa acgctgggag aacaagtata aaaagcttca acaagtcttt cggctacaag
    38581 aagaaatcat tgctacctat gaagcggctt atggcgaagc acccggactt attgaggaag
    38641 aagtacaagt aaaagaaagt gaaggaaaca gctctttctt taaaatgatg gagaaacgtt
    38701 cctataatcg cttcaatcaa gaacaaaaag cagctatcac atatgatatg aagaaacatc
    38761 ttcgaatcat agcaggagct gggagcggca aaacgcaaac aatctgtgca aaagctgttt
    38821 atctgatgac gcaaaaacaa gtagacgaag aacgcatact aatgatcact tttacaagga
    38881 acgctgcaaa tgaactaaaa aaacgagtag ataatttcag ccagagaaaa accgctgtcc
    38941 atatcggaac attccatagt atctttttta ggatatacaa tgaaatttgt cgaaaatttc
    39001 ctgaagtagc aatgcaagga atacaaggag acttttcaaa ggatactgca caaaaagtga
    39061 atgctgtttt gcaacagctg atacgtaaat ataatcttta tatatttgat caatatggag
    39121 aaaaaacgat tgcttctcgg ttagactatt ggcagaatat gaacctttca gtagaagaaa
    39181 tggttcagtt agttaaagaa aaatatgatt ccattgataa gaatgcacga cagccgatat
    39241 cagaacgact gtatggattg ctcacggaac tccaagaaca aaaaagaagc cagcaattgc
    39301 tagagttcaa tgatgtactc caaaatctga aaagcgcttt ggagaatgag gagatccagc
    39361 attatatcgg ccaaaaatat gattatctat tcattgatga atttcaagat actaatccac
    39421 ttcaatggga aatcgtgaaa cggatgacaa aggaaaacag gatcaagttg attgttgttg
    39481 gagatgatga tcagagtatc tatggattca gaggatcaga gccagcatac atcaagaact
    39541 ttgaaaaaga atatcctacc aaaactcttt tcttattaac taattatcgt tctcgggcag
    39601 ctattgtcca aggagcaaat agattgatca gctataataa aaatgatcgg ataccaaagg
    39661 cgatgattcc agcacaaaaa gaagcaggtg ttatggaagc ctatttgttt tctgatacta
    39721 aagcagaaag tcagtggcta atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat
    39781 ataaggaatc aatcatatta taccgatcag cttcacaaac gcagcagctt gtccaatctc
    39841 tgctgaaaac gaatattcct tttgtactgg aagcagatag tccatatgaa ggtatctttg
    39901 gtatcaagtc tttccaacat ttctatcaaa aaattgttca ttggcaaaca gcactgaatc
    39961 tgcaaaagaa aaatcaggcc taccagcaat tgctccggca gctgatggca gactgctacc
    40021 tgaaaaaaag tgaatgcgat caatactttt ctcctaaata tcaaaatata gccgttgcag
    40081 attatatttt gtcgataagc cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg
    40141 aggaagcgtt gcgtaaaaca gaccagcatc aagaaatcta ttcgcttgtt caaagctatc
    40201 ttcatctgcc acgtatagct aaggaaatcg accagagtga tcaagaatgg atacgagaag
    40261 aagtaaagca gcatgctact ttcagaagac tccaagcctt atcgcaagaa acccaagcga
    40321 cagctaaaga attaaaaacc cgcttgattg cttacagaca aggaaaactt gatgcactat
    40381 gtatccagag cattcacaaa agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt
    40441 gtaacgaagg aagtttgcct tataacggag cgacagaaaa gaaagtaatc gactttaatg
    40501 aaataaaagc agaaccagta acgacgatag aagaagaacg aagactattt tatgttgcga
    40561 tgactagagc aagaaatcgt ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat
    40621 tgaaagtatc gagatttatc aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga
    40681 agagagggtg tgacagaagt ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc
    40741 ttctcaaatt tttgtgaaat ttcagcttat tttcgaagaa gctatttctg gaacacagtt
    40801 tattcggaaa agg
//
LOCUS       DASJON010000006        34574 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJON010000006 DASJON010000000
VERSION     DASJON010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.006"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(178..2070)
                     /locus_tag="U3989_000220"
     CDS             complement(178..2070)
                     /locus_tag="U3989_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1713695.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="U3989_000221"
     CDS             complement(2067..3794)
                     /locus_tag="U3989_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1713696.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="U3989_000222"
     CDS             complement(3810..4460)
                     /locus_tag="U3989_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1713697.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="U3989_000223"
     CDS             4664..4960
                     /locus_tag="U3989_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1713698.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5132..5752
                     /locus_tag="U3989_000224"
     CDS             5132..5752
                     /locus_tag="U3989_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN1713699.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="U3989_000225"
     CDS             complement(5844..7010)
                     /locus_tag="U3989_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1713700.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="U3989_000226"
     CDS             complement(7022..7375)
                     /locus_tag="U3989_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1713701.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="U3989_000227"
     CDS             7503..8525
                     /locus_tag="U3989_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN1713702.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8544..8705
                     /locus_tag="U3989_000228"
     CDS             8544..8705
                     /locus_tag="U3989_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713703.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8964..9398)
                     /locus_tag="U3989_000229"
     CDS             complement(8964..9398)
                     /locus_tag="U3989_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713704.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="U3989_000230"
     CDS             complement(9508..11367)
                     /locus_tag="U3989_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1713705.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11485..12645)
                     /locus_tag="U3989_000231"
     CDS             complement(11485..12645)
                     /locus_tag="U3989_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN1713706.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..14376)
                     /gene="araA"
                     /locus_tag="U3989_000232"
     CDS             complement(12952..14376)
                     /gene="araA"
                     /locus_tag="U3989_000232"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN1713707.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14392..15090)
                     /locus_tag="U3989_000233"
     CDS             complement(14392..15090)
                     /locus_tag="U3989_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1713708.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15047..16708)
                     /locus_tag="U3989_000234"
     CDS             complement(15047..16708)
                     /locus_tag="U3989_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN1713709.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16926..18326
                     /locus_tag="U3989_000235"
     CDS             16926..18326
                     /locus_tag="U3989_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN1713710.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18578..19657)
                     /locus_tag="U3989_000236"
     CDS             complement(18578..19657)
                     /locus_tag="U3989_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1713711.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19743..20702)
                     /locus_tag="U3989_000237"
     CDS             complement(19743..20702)
                     /locus_tag="U3989_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1713712.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20714..22225)
                     /locus_tag="U3989_000238"
     CDS             complement(20714..22225)
                     /locus_tag="U3989_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1713713.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22247..22810)
                     /locus_tag="U3989_000239"
     CDS             complement(22247..22810)
                     /locus_tag="U3989_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN1713714.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22816..23679)
                     /locus_tag="U3989_000240"
     CDS             complement(22816..23679)
                     /locus_tag="U3989_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1713715.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23693..24589)
                     /locus_tag="U3989_000241"
     CDS             complement(23693..24589)
                     /locus_tag="U3989_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1713716.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24764..26053)
                     /locus_tag="U3989_000242"
     CDS             complement(24764..26053)
                     /locus_tag="U3989_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1713717.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26025..27542)
                     /locus_tag="U3989_000243"
     CDS             complement(26025..27542)
                     /locus_tag="U3989_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1713718.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27740..28630
                     /locus_tag="U3989_000244"
     CDS             27740..28630
                     /locus_tag="U3989_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN1713719.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="U3989_000245"
     CDS             complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="U3989_000245"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN1713720.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29539..31011)
                     /locus_tag="U3989_000246"
     CDS             complement(29539..31011)
                     /locus_tag="U3989_000246"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN1713721.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31648..32010)
                     /locus_tag="U3989_000247"
     CDS             complement(31648..32010)
                     /locus_tag="U3989_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1713722.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32233..32985
                     /locus_tag="U3989_000248"
     CDS             32233..32985
                     /locus_tag="U3989_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1713723.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33125..34048)
                     /locus_tag="U3989_000249"
     CDS             complement(33125..34048)
                     /locus_tag="U3989_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1713724.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34063..34335)
                     /locus_tag="U3989_000250"
     CDS             complement(34063..34335)
                     /locus_tag="U3989_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713725.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10218 a         7196 c         5938 g        11222 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    13861 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    13921 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    13981 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14041 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14101 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14161 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14221 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14281 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14341 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14401 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14461 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14521 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14581 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14641 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14701 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14761 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    14821 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    14881 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    14941 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15001 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15061 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15121 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15181 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15241 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15301 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15361 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15421 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15481 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15541 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15601 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15661 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15721 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15781 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    15841 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    15901 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    15961 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16021 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16081 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16141 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16201 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16261 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16321 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16381 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16441 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16501 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16561 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16621 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16681 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16741 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16801 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    16861 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    16921 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    16981 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17041 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17101 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17161 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17221 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17281 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17341 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17401 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17461 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17521 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17581 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17641 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17701 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17761 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    17821 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    17881 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    17941 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18001 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    18061 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18121 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18181 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18241 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18301 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18361 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18421 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18481 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18541 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18601 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18661 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18721 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18781 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    18841 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    18901 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    18961 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19021 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19081 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19141 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19201 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19261 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19321 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19381 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19441 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19501 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19561 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19621 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19681 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19741 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19801 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    19861 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    19921 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    19981 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20041 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20101 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20161 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20221 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20281 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20341 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20401 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20461 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20521 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20581 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20641 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20701 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20761 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    20821 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    20881 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    20941 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21001 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21061 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21121 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21181 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21241 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21301 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21361 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21421 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21481 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21541 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21601 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21661 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21721 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21781 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    21841 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    21901 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    21961 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22021 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22081 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22141 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22201 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22261 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22321 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22381 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22441 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22501 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22561 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22621 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22681 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22741 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    22801 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    22861 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    22921 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    22981 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23041 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23101 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23161 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23221 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23281 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23341 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23401 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23461 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23521 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23581 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23641 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23701 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23761 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    23821 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    23881 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    23941 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24001 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24061 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24121 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24181 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24241 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24301 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24361 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24421 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24481 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24541 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24601 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24661 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24721 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24781 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    24841 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    24901 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    24961 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25021 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25081 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25141 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25201 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25261 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25321 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25381 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25441 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25501 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25561 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25621 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25681 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25741 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25801 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    25861 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    25921 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    25981 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26041 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26101 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26161 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26221 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26281 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26341 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26401 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26461 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    26521 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26581 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26641 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26701 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26761 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    26821 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    26881 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    26941 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27001 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27061 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27121 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27181 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27241 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27301 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27361 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27421 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27481 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27541 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    27601 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    27661 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    27721 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    27781 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    27841 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    27901 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    27961 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
    28021 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    28081 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    28141 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    28201 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    28261 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    28321 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    28381 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    28441 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    28501 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    28561 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    28621 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    28681 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    28741 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    28801 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    28861 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    28921 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    28981 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    29041 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    29101 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    29161 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    29221 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    29281 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    29341 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    29401 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    29461 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    29521 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    29581 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    29641 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    29701 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    29761 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    29821 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    29881 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    29941 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    30001 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    30061 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    30121 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    30181 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    30241 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    30301 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    30361 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    30421 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    30481 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    30541 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    30601 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    30661 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    30721 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    30781 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    30841 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    30901 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    30961 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    31021 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    31081 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    31141 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    31201 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    31261 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    31321 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    31381 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    31441 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    31501 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    31561 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    31621 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    31681 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    31741 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    31801 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    31861 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    31921 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    31981 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    32041 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    32101 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga
    32161 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    32221 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    32281 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    32341 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    32401 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    32461 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    32521 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    32581 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    32641 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    32701 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    32761 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    32821 cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    32881 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    32941 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    33001 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    33061 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    33121 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    33181 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    33241 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    33301 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    33361 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    33421 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    33481 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    33541 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    33601 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    33661 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    33721 cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt
    33781 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    33841 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    33901 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    33961 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    34021 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    34081 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    34141 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    34201 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    34261 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    34321 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    34381 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    34441 aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc tttattatta
    34501 gtgaatggat atttactttt aatagataaa atattataac tattaaaaat aactattttc
    34561 attataatta caca
//
LOCUS       DASJON010000007        32497 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJON010000007 DASJON010000000
VERSION     DASJON010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.007"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="U3989_000251"
     CDS             complement(<1..563)
                     /locus_tag="U3989_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN1713726.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="U3989_000252"
     CDS             complement(750..1400)
                     /locus_tag="U3989_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713727.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U3989_000253"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U3989_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN1713728.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="U3989_000254"
     CDS             complement(2912..3910)
                     /locus_tag="U3989_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN1713729.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U3989_000255"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U3989_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN1713730.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="U3989_000256"
     CDS             complement(4926..5657)
                     /locus_tag="U3989_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN1713731.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="U3989_000257"
     CDS             complement(5661..6926)
                     /locus_tag="U3989_000257"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN1713732.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U3989_000258"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U3989_000258"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN1713733.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U3989_000259"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U3989_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN1713734.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="U3989_000260"
     CDS             12268..13770
                     /locus_tag="U3989_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN1713735.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="U3989_000261"
     CDS             13772..14509
                     /locus_tag="U3989_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1713736.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="U3989_000262"
     CDS             complement(14680..15873)
                     /locus_tag="U3989_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1713737.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="U3989_000263"
     CDS             complement(15952..16158)
                     /locus_tag="U3989_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN1713738.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="U3989_000264"
     CDS             complement(16151..17293)
                     /locus_tag="U3989_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1713739.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="U3989_000265"
     CDS             17805..18440
                     /locus_tag="U3989_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1713740.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="U3989_000266"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="U3989_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="U3989_000267"
     CDS             18916..19032
                     /locus_tag="U3989_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN1713741.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="U3989_000268"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="U3989_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN1713742.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="U3989_000269"
     CDS             21086..21253
                     /locus_tag="U3989_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1713743.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="U3989_000270"
     CDS             complement(21313..21666)
                     /locus_tag="U3989_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1713744.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="U3989_000271"
     CDS             22170..22589
                     /locus_tag="U3989_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1713745.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="U3989_000272"
     CDS             22615..22821
                     /locus_tag="U3989_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1713746.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="U3989_000273"
     CDS             complement(23049..23222)
                     /locus_tag="U3989_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713747.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23198..23863
                     /locus_tag="U3989_000274"
     CDS             23198..23863
                     /locus_tag="U3989_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1713748.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="U3989_000275"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="U3989_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN1713749.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="U3989_000276"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="U3989_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN1713750.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="U3989_000277"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="U3989_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN1713751.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="U3989_000278"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="U3989_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN1713752.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /locus_tag="U3989_000279"
                     /pseudo
     CDS             complement(29076..30241)
                     /locus_tag="U3989_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(30257..30484)
                     /locus_tag="U3989_000280"
     CDS             complement(30257..30484)
                     /locus_tag="U3989_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN1713753.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31558
                     /locus_tag="U3989_000281"
     CDS             30968..31558
                     /locus_tag="U3989_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN1713754.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            31748..32461
                     /locus_tag="U3989_000282"
     CDS             31748..32461
                     /locus_tag="U3989_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713755.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DASJON010000008        32027 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJON010000008 DASJON010000000
VERSION     DASJON010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.008"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1389)
                     /locus_tag="U3989_000283"
     CDS             complement(<1..1389)
                     /locus_tag="U3989_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN1713756.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKK"
     gene            complement(1376..2407)
                     /locus_tag="U3989_000284"
     CDS             complement(1376..2407)
                     /locus_tag="U3989_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1713757.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            2438..2659
                     /locus_tag="U3989_000285"
     CDS             2438..2659
                     /locus_tag="U3989_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN1713758.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(2824..3561)
                     /locus_tag="U3989_000286"
     CDS             complement(2824..3561)
                     /locus_tag="U3989_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN1713759.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(3714..5054)
                     /locus_tag="U3989_000287"
     CDS             complement(3714..5054)
                     /locus_tag="U3989_000287"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN1713760.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(5291..6814)
                     /locus_tag="U3989_000288"
     CDS             complement(5291..6814)
                     /locus_tag="U3989_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN1713761.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(7192..7803)
                     /locus_tag="U3989_000289"
     CDS             complement(7192..7803)
                     /locus_tag="U3989_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1713762.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(7926..8051)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(8133..8849)
                     /locus_tag="U3989_000290"
     CDS             complement(8133..8849)
                     /locus_tag="U3989_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1713763.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(8855..9424)
                     /gene="scpB"
                     /locus_tag="U3989_000291"
     CDS             complement(8855..9424)
                     /gene="scpB"
                     /locus_tag="U3989_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN1713764.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(9408..10205)
                     /locus_tag="U3989_000292"
     CDS             complement(9408..10205)
                     /locus_tag="U3989_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN1713765.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(10175..10546)
                     /locus_tag="U3989_000293"
     CDS             complement(10175..10546)
                     /locus_tag="U3989_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1713766.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(10565..11452)
                     /gene="xerD"
                     /locus_tag="U3989_000294"
     CDS             complement(10565..11452)
                     /gene="xerD"
                     /locus_tag="U3989_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN1713767.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(11548..12021)
                     /locus_tag="U3989_000295"
     CDS             complement(11548..12021)
                     /locus_tag="U3989_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1713768.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(12126..12989)
                     /locus_tag="U3989_000296"
     CDS             complement(12126..12989)
                     /locus_tag="U3989_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1713769.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(13185..15284)
                     /locus_tag="U3989_000297"
     CDS             complement(13185..15284)
                     /locus_tag="U3989_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1713770.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(15504..16613)
                     /gene="rpoD"
                     /locus_tag="U3989_000298"
     CDS             complement(15504..16613)
                     /gene="rpoD"
                     /locus_tag="U3989_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN1713771.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(16637..18478)
                     /locus_tag="U3989_000299"
     CDS             complement(16637..18478)
                     /locus_tag="U3989_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1713772.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(18660..20975)
                     /locus_tag="U3989_000300"
     CDS             complement(18660..20975)
                     /locus_tag="U3989_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1713773.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(21160..22020)
                     /locus_tag="U3989_000301"
     CDS             complement(21160..22020)
                     /locus_tag="U3989_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN1713774.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(22079..22600)
                     /locus_tag="U3989_000302"
     CDS             complement(22079..22600)
                     /locus_tag="U3989_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN1713775.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            22878..23639
                     /locus_tag="U3989_000303"
     CDS             22878..23639
                     /locus_tag="U3989_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1713776.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            23665..26256
                     /gene="mprF"
                     /locus_tag="U3989_000304"
     CDS             23665..26256
                     /gene="mprF"
                     /locus_tag="U3989_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1713777.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(26444..27631)
                     /gene="tuf"
                     /locus_tag="U3989_000305"
     CDS             complement(26444..27631)
                     /gene="tuf"
                     /locus_tag="U3989_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1713778.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            27999..28355
                     /locus_tag="U3989_000306"
     CDS             27999..28355
                     /locus_tag="U3989_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1713779.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(28596..29600)
                     /locus_tag="U3989_000307"
     CDS             complement(28596..29600)
                     /locus_tag="U3989_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN1713780.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            29766..30617
                     /locus_tag="U3989_000308"
     CDS             29766..30617
                     /locus_tag="U3989_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1713781.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            30634..30879
                     /locus_tag="U3989_000309"
     CDS             30634..30879
                     /locus_tag="U3989_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713782.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            31066..31770
                     /locus_tag="U3989_000310"
     CDS             31066..31770
                     /locus_tag="U3989_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713783.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         9095 a         6686 c         5536 g        10710 t
ORIGIN      
        1 ttttttaaat aaatttagta aaatcgttcc ccaatgaagt tgttcagtct tagaaaaaag
       61 cttcctcttt ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt
      121 ttcggagagt ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt
      181 aatatagcga agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg
      241 ttccacccaa ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag
      301 ctcatcaaag ggagcttgct ctgtggcata ttccaaataa agctcaaaac tttgtcgctg
      361 ttctctagaa agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag
      421 aattgctgca ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc
      481 tagactgtct ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat
      541 accaaatgca ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag
      601 ttgtcttctt tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct
      661 gaataaatga tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc
      721 ttgtgctctt tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa
      781 aagcgagata ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag
      841 tacatgacgt atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac
      901 cgggtttcct aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac
      961 tccccattga gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt
     1021 cagatgctct aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata
     1081 ttgcgataga tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc
     1141 agctactcgt ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag
     1201 cagcggtgac acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag
     1261 gctttttccc gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg
     1321 aataatttct tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt
     1381 tattaacatt ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc
     1441 cataatctaa ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct
     1501 tatttggttc caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat
     1561 caaaaaacaa ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga
     1621 ttgccatgat ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc
     1681 tttgattgaa attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc
     1741 gtttttcgat ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaaccgctg
     1801 actcatcatt gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata
     1861 attggttagc taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac
     1921 tctcatacgc agtatcaatc aaaatttcac gtggcaattg gctttcacta agccattttt
     1981 ttagttggaa agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg
     2041 ccagattaga aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc
     2101 tgccatagcg gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag
     2161 cccctgcagg cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa
     2221 ttttttgcaa aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa
     2281 acaaaagttc gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga
     2341 gttggtaaag agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa
     2401 caatcatatt attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt
     2461 acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta
     2521 tgatgaagaa ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat
     2581 gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt
     2641 attagaaaat aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa
     2701 cttgaaaata agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag
     2761 attcatttgc tatctatagt taattgacta gttttttcac ggaccctata ggacccattt
     2821 atattatttc aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc
     2881 tgcgttagaa atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc
     2941 atgatttccg ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc
     3001 ttgataagct tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac
     3061 agctgattct gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt
     3121 taaatgtttg tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga
     3181 atggccaccg atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt
     3241 tccgcccatc actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt
     3301 tgctaaatct tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa
     3361 aataagtaat ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc
     3421 tttgtttttt ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg
     3481 ctgtatatag taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag
     3541 tattcgtcta aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt
     3601 aatcaagtat gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa
     3661 taaaaaaaga cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta
     3721 gcacataaac gatacacagc ttctgcataa atttccattg ctttatacat actacttaat
     3781 tcccatcgtt cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat
     3841 gccacacact ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc
     3901 atgtctccag taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt
     3961 ggcacgtata atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct
     4021 gttttagtta atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct
     4081 acaccgattt ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg
     4141 ccggattgtt catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact
     4201 aattttccta agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga
     4261 cgagaaatag cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg
     4321 acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact
     4381 tcagaaagct tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca
     4441 gcttttacag aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt
     4501 tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct
     4561 ttttcgttat atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa
     4621 cgaatacgtg tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc
     4681 gcaatagaag gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata
     4741 gttggatcga atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata
     4801 tgacacaaga taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct
     4861 tctggatctt taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa
     4921 gcttcaatca cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg
     4981 gaaggaattc ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc
     5041 acaaattttt tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc
     5101 cgacacaacc tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa
     5161 tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttatccac
     5221 gtgcttatcg atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat
     5281 ttgtctaggt ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata
     5341 tcgcaattaa tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta
     5401 cagcacccat tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt
     5461 gataagcagt gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga
     5521 ttcccattgt tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat
     5581 agaaaataaa tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc
     5641 cccaatgtaa gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag
     5701 caacagctac tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga
     5761 caaattcaga ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata
     5821 acatagtaat ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg
     5881 tacctagtac cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg
     5941 cattcaaggt tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca
     6001 atacattaac atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat
     6061 cgaaatgctt catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt
     6121 cgatctttga tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca
     6181 tcaagaatcg aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc
     6241 ctgttgcaaa aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga
     6301 ttgctacagc tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat
     6361 agaatggaat cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca
     6421 tcaatactgg aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc
     6481 cggcatctag tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga
     6541 agaaagagat tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta
     6601 gcatcccatt gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc
     6661 gttcatatga tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta
     6721 taaaccaagt aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag
     6781 gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag
     6841 agacaacaat tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata
     6901 acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt
     6961 ttcaggtttt taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa
     7021 cattttcgtc ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat
     7081 gcataaatca aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt
     7141 gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac
     7201 tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc
     7261 atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat
     7321 cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa
     7381 aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag
     7441 agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac
     7501 actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc
     7561 tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa
     7621 cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc
     7681 actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa
     7741 aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt
     7801 catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa
     7861 tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa
     7921 ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc
     7981 tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc
     8041 tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa
     8101 cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg
     8161 acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct
     8221 ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc
     8281 acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga
     8341 tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct
     8401 tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact
     8461 ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt
     8521 agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc
     8581 atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact
     8641 cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc
     8701 acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt
     8761 acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg
     8821 tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa
     8881 agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca
     8941 gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata
     9001 tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac
     9061 gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg
     9121 gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg
     9181 tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat
     9241 ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta
     9301 attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt
     9361 cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg
     9421 tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt
     9481 tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact
     9541 tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt
     9601 atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt
     9661 ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa
     9721 tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg
     9781 ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca
     9841 gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct
     9901 tcttcccata attcttcatc gtctgtgacg aattcttgga caggaagcaa tgtcttgctt
     9961 ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt
    10021 gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca
    10081 tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat
    10141 acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta
    10201 gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact
    10261 tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca
    10321 aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt
    10381 gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt
    10441 acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata
    10501 gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct
    10561 atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat
    10621 gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca
    10681 aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta
    10741 cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc
    10801 gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa
    10861 gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag
    10921 gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa
    10981 gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt
    11041 ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct
    11101 tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga
    11161 tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc
    11221 tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct
    11281 ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct
    11341 gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca
    11401 atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc
    11461 ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc
    11521 tgagcaaaga aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata
    11581 gcattcctga cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg
    11641 ttcttctact acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc
    11701 gcagttcaag catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata
    11761 acgggctaat ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc
    11821 aagggttcga taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa
    11881 gatttcttct gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc
    11941 tctttgcggt gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc
    12001 cgaaatagca ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa
    12061 aaagatataa cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg
    12121 gattctcaat tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct
    12181 tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc
    12241 atgatttctt ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct
    12301 aaaatggtta aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat
    12361 gaaagattca gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct
    12421 agacgaggtt cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt
    12481 aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc
    12541 atttcttctg ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc
    12601 caaatacggt cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc
    12661 catagctctg tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga
    12721 ccgatatcga caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaagcgtaa
    12781 tgaccgatac ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg
    12841 taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca
    12901 aaggtttgtc cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct
    12961 gtaaatatag ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct
    13021 taatacattt ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt
    13081 tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat actccccttt ttcgaataaa
    13141 cggtgttaaa cggcaggctc acagcctctt tcatttgcct aaaattaatt gctcccattt
    13201 cctccgaacc atccagtttt acagttgatg gttacgatgt aaaatggcaa agcactttct
    13261 ccaatgcctt tcatcaatgc agattttgtt gaagtggcat atagattata atagccttcc
    13321 ccattttcga ttctggcttt ttctaatctg taacctcctt caacgatata tccacgttgg
    13381 atacatgtag caatcacttt gctttggaca cctggtgccc acgtccatgc tggattagat
    13441 gcatcggcaa tatcacttgc tctggcagat ttgattggct gattcgctga gtttgaagag
    13501 gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg
    13561 gcttgagctg cagctgctgc ttgagcggct tgttttgctt gttcttcagc tgcttttttc
    13621 gcttcttctg cttttttctt ttcctcttct tcttttttct ctttatcagt gagcatctta
    13681 tctacttgtt tcaattgttc attcaactga gcagatagat ctgtattttt cactttttct
    13741 actgcttctt ttgccgtctt gtattggtcg cgtgttgctg aatccactac ttttccgtct
    13801 ttaaagacta tagctgtttt ttctttagct gtttgcaatt cagcatattg atttttagct
    13861 tctgtttgtg cttgttgaat caattttcca aaatctgtgt tatccgtttc gattgcttgg
    13921 atctctttat ctgctttcaa cttgatgtca tctgctagat gatcatcgac gatggcaggt
    13981 gctatgaacc actcattcac tttttggata tctcggatct tagattggat atcttggtaa
    14041 gttgtttcta actctgaata tcctttttca ttcttaagac tggataaatc attttttagt
    14101 tttgataaat cttgatgaat catatctgca cgaataaatt gatggtgatc atctgtataa
    14161 aaggcagcaa gtttctcatg aagttcttcc atttgttgct gtctttttgc ttctgctgct
    14221 acttcggctt ctactttttg atgatggtca taataagccc agcttcctcc accgagtaga
    14281 agtactgctg ctaaaacgag ataaggtact ttttttgatt ttttcttttg cgctttatct
    14341 ggatctgttt tttctaagtt tgattcttga tgtgtttttt cagtatctgg cacgattgtt
    14401 attgatttcg cttctgcttc aaacagctta ggctggttct cttctttttc tatttttgct
    14461 tcaagatcag taccctcttt gcttacagat tgattttcag catcggattc actatttatt
    14521 tcatcgggct ctgttgactt tttgtctgtg ttttcgagta tttctttttt ctctgcttcc
    14581 ggcagtttat tcgttttttc cttttctaat tgtttttcag taaatacttc agttttttct
    14641 gtgatatccg tttttacgtc ttttattttt actgtttctt tacctgtggc gttttctcta
    14701 tcagagtctt tttcagtttc ttgttccttt tctggctcta ctttgttttc ctgtgcaatt
    14761 ttttcactga tcattttgag cagctctttc gcgtgctctt cttttgtatc ttcacgatgt
    14821 gctttgatat aagcggatag tatcgaattt tcttcctcat tatcgacgat ttctttcagt
    14881 tcagctactc ttcttttttg ttttgattca agcgtttctt cttgattctt tcctgattct
    14941 tccgaagtct catgaacagt tgctttcttc tgttcatttt tctctatatt agttacttga
    15001 tcgtgagcag gtggttcttc gtcttctggt tctgcttttt cgaataattc cattacagat
    15061 tctagaggta aatctttcaa ttccgaccat tcgattggat cgtttaattc tgtatcagga
    15121 aagactgcat cttcagttga tttatcctgc atagctgcat ctgtatttgt ttcttcaatt
    15181 gattctttta tttgctgatc ttctgcagtt atttgatgac cgcagtctgg acagatagta
    15241 ttctgctcca tttttttcga tccgcaattt ggacattttt tcatcactta gcgatcggct
    15301 cctttactag gtttacgcta ccatttaatc tatcatattc tatacgtcac gccaatagct
    15361 tatttttagg atttacttaa aaataatgaa tgaatttcct tcctatagaa aaaaaagctt
    15421 gagactgcgt agtctcaagc tttttttctt ttaaaataaa ccaaacagtt ttactgattt
    15481 ctcttctggt caatagtcca cgcttattct aagaaatctt ttaattgttt tgaacgagaa
    15541 ggatggcgca attttcttaa tgctttagcc tcgatctgac gaatacgttc ccgagtaaca
    15601 ccaaatactt tccctacttc ttccaatgtt ctcgtccggc catcatctaa accaaaacgt
    15661 aagcgaagga cattctcttc ccgatcagtc aatgtatcta gtacatcttc caattgttct
    15721 tttagtaact cataagctgc gtgttctgct ggacttgttg cttcctggtc ttctatgaaa
    15781 tcacctaagt gagagtcatc ttcttcaccg attggcgttt caagggaaac aggttcttgc
    15841 gcgattttca agatttctcg gactttttct gttggaagat ccatttccgc accgatttct
    15901 tctggcgtag gttctcttcc aaggtcttgc agcaattgac gttggatacg tatcaattta
    15961 ttaattgttt caaccatatg aactggaata cggatcgtac gtgcttggtc agcgattgct
    16021 cgagtgatgg cttgacgaat ccaccatgta gcataggttg aaaacttaaa tcctttgcgg
    16081 tagtcaaatt tttcaactgc cttcatcagt cccatgtttc cttcttggat caagtcaagg
    16141 aactgcatgc ctcgtcctac ataacgttta gcgatactta ctactaaacg aaggtttgct
    16201 tctgccagac gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc taacgctact
    16261 tcttcttccg cagttaaaag ggagacacgg ccaatttctt tcagatacat acgaaccggg
    16321 tcattgattt ttacaccggt tggcgcagaa agatcttctg tttttgcttg ttcagctttt
    16381 ttctcagcac tcttcaagct gtgaattgat ggatcaccat tttcatccac cacactgata
    16441 cctgcgtctt caatcttttg gatcaatttg tccatttcat ctgcattcaa agtgaatggt
    16501 gtagcaagct gattggataa atcatcatat acaacttgtc gtttgggttt gttttctttg
    16561 atgaaagctg ctactgctgc ttcatacgtt tttttatctg ttccatttgc cataaaagaa
    16621 ggcccccctt gagtagctat ttggcttgtt ttaactgttt cgttaaattg atgatttcaa
    16681 tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt
    16741 tttttaatgt tatttcatca gctaagccag atttgctgat cacatctagc aagtcttgga
    16801 tttctcgtgg cgaactttct tctgccaccg gtaaatattc gatttccacc accaaccgcc
    16861 ttaaattttc atcttgcaaa aagtctaaaa actctgcaag gttgaattca taatgttgtg
    16921 agacatatgc atcgaataaa aaataaactt ctgcatatgc atcatgaata aactgaaatt
    16981 ctcgttcttt taaaatatta cggacagaaa cttctttaaa tgcacgatat aatagaatct
    17041 gctctgcctt ttctacttgc gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa
    17101 cctgagtgtg ttgactttcc tgaggttctt gacgattttg ctgatttgtc cttcgaagat
    17161 cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa ctctgttgac agttgagata
    17221 gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt aattcttcga
    17281 ggtattcgat cttgtctttt tcattatcta aatttttccc ttgttttaaa taacgtgatt
    17341 taaaagtaaa gactgtttcg cgcccatgag ccactaattc ttggaaagat gcttctccat
    17401 acttacggag atattcatca ggatcaagct tttctggaac gactacgaca gaaagtgcta
    17461 atcgactgtt ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt
    17521 ctccgtcata gcaaaagacc aattctttcg tcagcttttc catttgttgg atctgttgag
    17581 ctgttagact tgtccccata gaagctaatc cagttttgat accagcctgc catgcagcaa
    17641 tcacatccat aaacccttcg aacaacaaaa tttctccgtt tttccggata tcgcttcttg
    17701 ctttatcaag attaaataaa acttgtcttt tattgaacag ttctgtttct ggactattca
    17761 agtatttggg ctgatctttt tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac
    17821 cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac tctttgataa aaacgatcag
    17881 acatacggcc atcatcatgc gtgacaaaca atccagagcg ttctaacaat tcatttgaaa
    17941 ttgattcatt cttgaatact tgctgaagaa aagaacgctc attaggtgca aaacctatgt
    18001 tgaattcttc aattaattct aaagacaacc cacgactttg caaatattct aaagctggct
    18061 gtccagcttt tgtatgcaat agcatgtgat gatagatttc cgctgctttt tcatgagcag
    18121 caatcagttt ccccgtagca gattcaggat ttcggacagc tatagtacgc tgacgccatt
    18181 gatcttctaa cggtatctgt tcgaagtctg ccacttttac cactgcttct ggaaaactta
    18241 gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat
    18301 gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt acgttcttca tgaaaaggac
    18361 acagccccaa gtaattttta ccagactttt tcaactggac atattgtcca atcacctcaa
    18421 cgatattcgt ttgactgcgt actgtttcga taacctcttg aggaatccgt tgagccattt
    18481 tttcaccccc tgctctattg atatcttttg ggtttaacag aagatatttc aggagaataa
    18541 ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta aaggacacaa ttatacatgt
    18601 ttgattgtat catatttcat tgctttttca agtataacac atttctatct cattaagcat
    18661 caatttgcta ctcttttcag taactgattt tgaatatcag ccttccagta caatactcga
    18721 agaacaatat ttacgagcat gaatacaaag aagtatccta cgctctctcc caaccctaag
    18781 tttacttcca aaattccatg gaacagcatt gcagctacat atatccctac gatcgtcgtt
    18841 actgctagga agatgcgcaa tgggttgaag ggataacaag cttttacaac tgccatgcaa
    18901 ctgatcccaa tcagcaagta atacatcaaa gtagtagttt cagcatttcc ccatgcgaat
    18961 gtttttccaa gtagatacac cgcaatgata ttcactacta caagcaaggc atttggtaaa
    19021 gcattaataa gtgctgtagg caaataacgg taagtgattt ttcttttatc ttgttcaaaa
    19081 gataaaaaga aggaaggata cccttcaatc gctaaatcaa tcagcgtgat ttggataggg
    19141 ataaacggaa atccaattgc tgtaacggca catataatcg ataaaataaa tgaataaatc
    19201 gttttgataa aaaagatgcc tgagactttc gtcacattgt tgaccactcg gcgtccttca
    19261 aacaggactt ctggcaatgt agtaaaatca gagtccaaca ataccagatt cgcaatctga
    19321 cgtgttgctc catcaccttc tgccatagca atgctgcaat ctgcttctct taacgccaaa
    19381 acatcattca caccgtcgcc tgtcattgca accgttctgc cgctttcttt caattcattt
    19441 accagcaatt tcttttgctg cggactcact cgtccaaaaa ccgtatagcg atgagcagct
    19501 tctcgcactt ccatttcttc agtaatttga gaaagatcga catacgcatc ataattcggt
    19561 aatcctgcac gtctagcgat attggagaca gtaaaaggat tgtctccaga aatcactttc
    19621 agatccacac cttcctcatg aagataagct agtgtttctt gagcgttttt tcggattgga
    19681 tcatcgattt caaacagagc aaccggctca acaaatgctg gtttttcttc gttcaattgc
    19741 tgatttggag cgatccccaa cataagtacg cgatagccat tcgcttgggc tgtttttaga
    19801 ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta cacgactccc
    19861 agttctggaa attccatcgc gccccatttg cgatcagaag aaaatgcgac tttgtttgtc
    19921 acatcatacc gattagaaag ctcaaaataa tctctcatag cccgcatagt gatgttgtta
    19981 tcactgcttt ctgccagata cgtccccata atttctggga tggctttctc gtacagcgga
    20041 tgtagtgctt ctacccgctg gatactcatt ttcccttcag taatggtccc tgttttatcc
    20101 aagcataatg tatcgacatg ggccagcgtt tcaattgaat acatgtcttg aacaaggatt
    20161 cgtttttttg ccagtttggt tacccctgtc gttaatgcaa tacttattag caataccaaa
    20221 ccctttggaa gcatgcctaa caaagctgct gcagatgcaa taacagaaat tttgatagaa
    20281 gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa
    20341 ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt cagaacgaat agctttgtga
    20401 gtctttgcct cgctagtcaa gcggacagca taattatctg cacctactcg gataacttca
    20461 gccatgacag aaccgcttgt caaaaagctt ccagaaagca attcagctcc agtttctttg
    20521 acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg tccagatata
    20581 acctgtaaat cggctggtat ctgttctcct gctccgattt ttaccaaatc tcctaatacc
    20641 agctctttag tagaaatcga ttgttcttta ccgtcccgga tcactagtac ttctgattgg
    20701 gaaacaatcg ataatttccg tacaagattt cttgcatgta tttcttgata aattccaata
    20761 acgatattga cgacaataat tgctaaatac gccatgttgg aatacgcacc tacaaataac
    20821 agacaaatcc caataccaaa gttcagaaaa ttgaataacg tcaatatatt atcgctgaat
    20881 atttgttttg tactttttga tgcatcttcc tggtagtcat tttgctggcc agaagccatt
    20941 ttctgaacta cctcttgctg tgtaagtcca ttcattttct ttttctccat tttccgccaa
    21001 ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca agattaaaaa caacttgttt
    21061 ttataaaatt tatagaaaaa cacacgaatg attttacaaa aaagatgcca acctttttgt
    21121 ttgaatcacc cgtgtgctgt ttttattatc aatctatctt tacttatatt gttcctccaa
    21181 acggctcatc caccaaccta gtgctgtacc ggctataaaa agaacaggac aagctagtac
    21241 cccagcaact cccaatcccg cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac
    21301 tacacctaaa atgaaatgga atagcttgct atatgccttt gagaaaatgt aatccatgat
    21361 ttttgacaat ccaagaacac aaagaatccc accaattccg atcggcacaa tcacttcaag
    21421 tcgtacttct ttaaatccgt cagccatcgc tttatacaat cccatataca ccaaaaaatt
    21481 agaagggctc aatcctggga cgatcatacc caaaccaatc aatccgccgg caatcagcca
    21541 tgttccaaaa ttctgatcga cttgcccgtc aaataattga gcgccgtata ataaaaacaa
    21601 ataagccaga acaaaagtaa caaccatgat gatcacatca gaagtatctc ttcctttttt
    21661 ccctgcttct ttccataacg caggaacagt tccagcgata catccgacaa aaaaccataa
    21721 tatcgttgtt tcatacgcac ctaataaaaa actgactaaa aaagaaagta gaaagatgcc
    21781 tgtcactccg cctagaccaa caggcagaaa aaacaaaaca ttttctttga aattttttgt
    21841 aaggtgggct aaaaaagaaa tcagccgctc atacatgcca aagacagctg ctaatgcacc
    21901 accgctgact cctggcaaga taaatcctga accgataaac attcccttca caaaccgtaa
    21961 catccagtct ttggttgtgt accccacttc ttgattttcc attttttgcc ttttttccac
    22021 tatttgcgta ctccttcttt catttaattt tattcatgat tgaacatttc ctgctatttt
    22081 acttaaagac tttttcatag gtctctatta ttttttcatt cattttgaca atatcaggat
    22141 aaaacatgtt acctccatta tgatagagat agccgccatt gtaaagcaag gactggatcc
    22201 gccaatatcg gtattgtttc ccttcctcat ttcctaaaag tgggctcagc acatctttag
    22261 aatacgcttc tgctaattct actgtgtttt ctccgccatg attttttaca taggcaatat
    22321 aatctttccc aaaattatat gcttggacag ctgtccataa atcgcatcct tgtttctttg
    22381 cttctactat catttccgcc aaatatttga caccttgatc aatactgtca gctggattat
    22441 caatgatctc agcttctcca tgcagacttt ccgagctctg catgagatct gtcccttttc
    22501 cttttgattc ggtcaatatt atcgccttga tcaaattctc gtagttttcc atatgataat
    22561 tttttgcagc tgttgttatt tctatttgat agctttccac tcgttttacc gaacgataag
    22621 ttcttcctca taaaaacaac accgcaccaa taaccgtcag aatgatcatg taaaaaagaa
    22681 tacggataat agataatcgt tttcgcttca tttcatcacc tagttatttt gacatctttt
    22741 tttatcttac tatttcttta tcttcttggc tatttcttcc ttgatttctg tgtacgcctt
    22801 aagaaaaaat gaattttcct gtattttttg acatcttacg aaaatgatcg ttgtttttca
    22861 gtaaaaacaa ttgcccaatg actgatataa tcgttatact ttacacagta ggtgattatg
    22921 tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga
    22981 gcggtcaagg cttccctctc tttttactgc atggcaatga cggcagcggc cgcttttttt
    23041 cagaacaggt ccctgtactc gaacgctact ataccgtgta tctagtagat agccgaggac
    23101 atggtcgttc gacaaacgaa gcatctatgt tgaattttca gttaatggcc gaggatctga
    23161 atacgatcat gttgctggaa aaaatcgatc aggcagattt tcttggattt agtgatggag
    23221 caaatctagc actggtattt gccagcagct tccctaaaaa agttcatcga ttgattttaa
    23281 attctggaaa cacgcttgtt aaaggcgttc gtttttctgc cagagtcatc agtaatattc
    23341 attatgcatg ggtatggtta ctctctttat ttcgcccaag tcttcgcaaa aatcttttag
    23401 ttatcaaact tttgctgcat gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact
    23461 cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata
    23521 tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa agaacaagga catgaattag
    23581 ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca atttctttct gaaacataac
    23641 tattaggaat agaaggtgat ttatttgtta aaaaatacca tacaatggct gaaaaaccac
    23701 ctaggactgt tcaaaacaat ctttttgatt tctgtcattg tcattattgt cggtgaattg
    23761 atgtccatcg gtaaaactct atctatccag caattaggag aaacgtttgc cactatacct
    23821 gtatggaaaa gcggactaat gctattgatt ggtcttatct ctgtgttacc tatggttggt
    23881 tatgatatta ttttgaatcg gatgcttgaa caaaagcaga atcctcgtta cctgtttgag
    23941 acaagctggc tgatcaatac gatcaacaat attgccggtt tcggcgggtt tgttagtgtg
    24001 gggcttcgct ctgaactata tggacaaaag aaagagagca gaaacgtgat ccaagcatta
    24061 tcgaaagttt tcttgttttt gatggctgga ctttctattt acagtctgct ttctttttta
    24121 cttgtagtct tcacgccagt tgctcctttt ttgaagcaat attggatatg gttgatcggc
    24181 ggcggtctat attttcctgt cgtcttttta tttactactt tcaaaaagaa aggattcctt
    24241 ggtggtacct cacttaaaac tcaaggacag ctattgctag tttcattgtt ggaatggacc
    24301 ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg aaatccggat tgatctgtgg
    24361 caaacaattc cattattcat tgctgcttca gtgataggaa tcgtgtctat gattccagga
    24421 gaaattggga gtttcgatgt aatgatgatt atcgggttgt ccgctattgg tgtgccaaga
    24481 gaaatagtcg ttgtgtggat cttgctctat cgcctgttct actacatcat tccgtttttg
    24541 attggaatcg tctttttctt caaaaatatc ggttccactt ttgatcagcg ttattctggt
    24601 atccctaaac aattagcaac agaaattgcg cataaaatcg tggttgtctt attatacttt
    24661 tctggaatca tgctagtact ttctgccact atcccgcaag cttttacaga gtttcgctgg
    24721 cttcatagtt tgaacccatt gaaatttcat tttattatcc aatttcctag tatactattg
    24781 ggatttcttt taatcattat gggcagaggg atcgcagcac gtgtgaaacg agcatatttg
    24841 cctacaatct tcttgattgt tctagcttta ctttatgttt tattaagtga ttttagtttc
    24901 acaccggtgg ttttcttgtc tattctgctt ctgatcattc ttgcttctaa aaacgaatta
    24961 tttcgggaac aattgatcta ttcatgggag tggcgtacga tcgatggaat cattattggt
    25021 gcactgtcct tgctttacat cgttattggg gtatacaatc tgccggattt ccctcatcgg
    25081 cgtcaccatt ttatttcctt tttcttattt ccttctgaaa agatttggtt ttcgggattg
    25141 ttggccatca tcgctgttag tttcatgatc gttctatttg ttcatttcct tcaaggagaa
    25201 aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc tgaagatcct tacaacttat
    25261 ggaggaaact ccgatagtca attgatcttc ttaaaagata agcggatgtt tgcctatgaa
    25321 aaagacggtg aaccaactgt tcttctccaa ttcgcttgct tcaataataa atgtattgta
    25381 atgggtgatc cttctggtaa gaaagaagat tttccagaag caatcgaagc ttttatcgaa
    25441 gaaacagatc gactatgcta tttaccagtt ttctacgaaa caagcgaaga gattgttatg
    25501 attttgcatg aattcggcta cgatttcatt aagatggggg aagaagctta tgtagacctg
    25561 aatagtttta caacttcagg gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc
    25621 gaacgagaag gatttacatt tgatgtctta caaccaccat tttctactga gcaaatgagt
    25681 actttcaaaa atatctctga taattggttg ggttcacgaa aggaaaaagg gttttcttta
    25741 ggctttttct ctgaagatta tctacaaaga gcgcctattg cagttgtcaa aaatacccaa
    25801 gaagaaattg tcgcttttgc aacgatcatg cctacttaca cgaacaataa agtaggaacg
    25861 attgatctga tgcgatatga ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc
    25921 ttgcatttgt ttgcctatat gaaagaagaa catatccaat ggttcaattt agggatggcc
    25981 cctctttcaa atgtcggaac ttccagaaag agttttttac aggaaaggat tgcatacttg
    26041 gtttacgagt ttggttctca tttttattct ttccatggat taagagaata taaaagcaag
    26101 tacgctacaa actgggtctc acgttatact ctatattcta gagatagttg gattgcatat
    26161 gttatgattg ctttgctgat tattgataat gcgccagttg agcagacaca aggtagaata
    26221 agtggtttga aaaaatggat acgaagaaag tattaaaaac tgcataccga tagatgaaca
    26281 ctctcatgat gaataaaaga gactgaagta aaacgaaagt tcatgcttct acaatctctt
    26341 cttatacaat tgaagaacaa agagcggtcc aaacagaaat cctgtttgga ccgctctttt
    26401 attttaaaca tttttcatta ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg
    26461 taacgatacc tgaacctact gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt
    26521 caacagcgat tggatggatc aagtcaacat caatcgtcac gttgtcacca ggcatgacca
    26581 tttctgtatc ttctggcaat gtgattgttc ctgtaacatc agttgtgcgg aagtagaact
    26641 gtggacgata gttgttgaag aatggagtat gacgtccacc ttcttctttt gtcaacacgt
    26701 acacttctgc tttgaatttt gtatgtggag taatggaacc tggtttagca agtacttgtc
    26761 cacgttcgat atcgtcacgt tggatccccc gtaacaacac gcctacgtta tccccagctt
    26821 ccccgtaatc taacgtttta cggaacattt ctacacctgt tacaactgct ttttgtgttt
    26881 caggtttgat ccctacgatc tctacctcat caccgacacg aacagcacca cgatcaatac
    26941 gacctgaggc aacagttcct cgacctgtaa ttgagaagac gtcttccact ggcaatagta
    27001 atggtttatc tgtatcacgt tctggtgttg ggatatattc atctactgta tccatcaatt
    27061 ccataatagc agcttcagca tctggatcgc cttgcaatgc ttttaatgcg gaacctttga
    27121 tcacaggagt gtcatcgcct gggaaaccat attcgctcaa taactcgcga acttccattt
    27181 ctaccaaatc gatcaattct tcatcatcga ctaaatcaac tttgttcaag aaaacaatca
    27241 ggtattttac accaacttgg cgtgacaata aaatgtgttc acgtgtttga ggcatcggac
    27301 cgtcagttgc agaaacaact aagattgcac catccatttg tgctgcccct gtaatcatgt
    27361 ttttcacata gtccgcgtgt cctggtgcat caatatgcgc gtagtggcgt ttttctgttt
    27421 catattctac gtgtgcggta ttgatcgtaa tcccacgttc acgttcttct ggagctgcat
    27481 caatactagc atagtcttga ggatttgcta ggcctttttt gcctaatact gtagtgatcg
    27541 cagcagttaa agtagttttc ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg
    27601 gtttacttct gtcataatgt tcttttgcca taatttacaa acctcctaaa taatatagat
    27661 cactgaaaaa tttcagctct caccttttta ttatagtctt tttttgacta aatcaaaaag
    27721 aatacgtccg acttactttt tgtaagattc gaactaagct tattatacgt caatattggt
    27781 caaagtcaac taagagcact cgtctgtctt atacgcgaaa aaagtacctt ttataaataa
    27841 aaaaatgtgt tttttgagaa aaacttcaag atagtcctta actttctttt gaagcaataa
    27901 gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa caactttcaa tagacgaaat
    27961 cttctatttc gttaataata aggaaagagg tgaagtacat gaaaaaatta attggcgtac
    28021 ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc ttatcattat ttttacggcg
    28081 gcgaagctta ttatacaaaa atcacgacga atggcgaaat gtcagctacc caagcagata
    28141 acggagagaa attcacgact tatacctaca agcaagcggc ttataataaa gatggtgaaa
    28201 aaaaacaggt gacgcttcgc gaagaaagag aacatccatt aaagatgaat gcgtatctta
    28261 aactcaaagt caatcctcgt aaaggtgtat taagctggga agaaataaag gcttctgaag
    28321 tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa ttttattaga
    28381 taaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct
    28441 tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtctcacgc
    28501 tctttttttg ttatattcgt cttttccgat ccttctcttg acacaattca tttaactgat
    28561 cactggtact tgaacaccta atattctagt catttttata aacgattcaa tacttcatcc
    28621 acatttttga tcatcacgga tatcgtacca tccggattat tcacaaattc aatcaaatct
    28681 gggttgcgat atacatctac tggtacgatc agttcgatcc cgtttgatag cttgaatttt
    28741 tgttttccaa attttttctc agaaatttct tgtacttctt ttacaggagg tgcttctggg
    28801 acaaatcctt gctcttgtac ttcttcttta aaagccattt ttgccgtgat gttttccttg
    28861 aagacttttt ccgcaatcat ctcatgatcc aaccgacctt tttcttctat cgtatcatag
    28921 acagcttctt tcaaatcagc catgatatca aattcttctg tctcaaattt ttttccaaga
    28981 tgcttggcta ctttttggat cacttttaca ttttcttcta aagaaggtgc aggaacactt
    29041 tcgatcactc ttccagaaaa ataaagctct tttttgccag aaaactcata ttttttttca
    29101 attaattcgt aggtccaatc agtcaaattc acagcgaatg cttcatctgc tttttgtgtt
    29161 ttcgccccta gaattgctcg attcaaaatc aagcggttgt cgatccctgc ttcgtctgct
    29221 tcaacaaagt gagtgtaagc ctcacgataa ttgactttca ataaagataa gtacatcact
    29281 gtatctaatt catacaagac aacaaataca tctgcactag gtgcttcttc actttcttgg
    29341 tagatatcga accaccgctg aacgattgct tcacttgctt ccacaaaatt ttcttgagcg
    29401 attttaagtg attcaacgat catgctttct tctgctagtt gtccagtttt tgtttgagca
    29461 gaagagattt tttggatctt cttttgaaga aaatcacgaa tatattcttt tgtcagatcc
    29521 agctcttttt gtgaaaagac tggagaacca gtttctctgt caataatatg cagaattgct
    29581 ttttttaaat agatatccat tttctcattc ccttctgtct tagtttacgg aaaaaaaatc
    29641 aaaaatacca gaatcaaaat cattttcttt taaacaaaaa tttcaagagt agaaacaaaa
    29701 tggattcttt ttttcgaaaa atgcgctaca cttatagttg aacgaataaa aaaggagctg
    29761 aaagtatgaa ttcattaaca agcacttatc gtctatcaaa tggttacgaa atcccggtag
    29821 taggattcgg cacatggcaa acacctgatg gggatgtcgc tgtttcctct gttaaagaag
    29881 cgctggctgc aggttatcgc catatcgata ctgcccaagg atacaagaat gaagaaagcg
    29941 tcggccaagc gattaaggaa agtggaattc ctagagaaga gatattcttg acaacaaaat
    30001 tatggaatgc aaaccacagc tatgaacttg tgatgtcttc ttttgaagaa tcattgaaga
    30061 agctccaaac agattattta gatctgtttt tgatccactg gccaaaccct gttgcttttc
    30121 gtgataattg ggaacaagca aatgcagata catggcgagc atttgaagaa ctgtatgaag
    30181 caggtaaaat caaagcaatc ggtgtgagca atttcttacc ccaccatcta gatacactag
    30241 caaaaacagc aaaaattatg ccgatggtca atcaagtttt ccttgcacca ggcgaattac
    30301 agacaactgt agttgagtat gcaaaaaaac atgatatgat cctcgaagca tatagtccat
    30361 taggcactgg taaaatcttc gatgtgcctg aaatgaaaca aatcgctgaa gcacatgaca
    30421 agacgattgc acaagttgct ttgcgttggt cgctgcaaca cgaattcctt cctcttccta
    30481 agtcagtgac accaagcaga atcaaagaaa atactgaatt atttgatttt gaattaacag
    30541 aagaagaaat gaaacaaatc gatcagttag atggtgtagt cggcaaagca aaagacccag
    30601 atactaccca attttaagtc gaaaggtgat tttatggatc caatcgaacg cttgaattct
    30661 ctctcggaag aagttaccca gacttttcat tccgattttg tctttttgat cgatgcagag
    30721 aaaatccaac attttcctgc aagaaactgg acacatgatc aaatcatcga agaactgaaa
    30781 aaacggttcg atcattcgct aatggtcaaa ccctggcacg aacatgaagt catttattca
    30841 cctgaactac ctgtttttgc cttgataccg aaaaaataga aagaatggaa cggattatgt
    30901 atatatacat aatccgttcc attttttatt tttctttcaa aaaacgggaa aatacgcaaa
    30961 tttttctgtt gtaatttgcg tatacgcaag tttttttgca ttactccttt tcatttggct
    31021 gtttttttct tataatcgaa agggtatgag cgaaggagag aacatatgaa gaaaaaacag
    31081 aaacaaaaat tattaaatca attccgtcct tcattggatg gtgtaagagg acaattattc
    31141 cgttctctag aagaagagag cttgcgacgt tatggtttgc cgttacaagt catgcttgat
    31201 cccaaaaacc gtcaaatctt aacgatcgga ttagctggga aaacaaatga agatacacgg
    31261 atcaacgtgc caatcgatga caacttcaat actgtattaa agagaattcg ttcaggagaa
    31321 aacgggattt tcgaacgatt ccgtgacaat cttcttattg aaatcgtgtc ttactggaat
    31381 gatcaacgat tgaaaaacga tgaaccaact gcgcaagctg ttgcgacacc agttacagaa
    31441 gaaacagtca gtgtgccaga agaaaaaacg gaaaaaccaa aagcggacac aactgaaaat
    31501 acacgctcaa ctgatacgaa tgccgcttta agttttgctg atttttcaaa agccgttgct
    31561 gagtatccta aattttacac agaacaaaat gaacatgaag tagttattaa tgaaaaaaat
    31621 gctgatgaac ctcgtaaatt agcaactatt tctatgacta ctgaaaatga gttcgttatc
    31681 gaaaaagcac tcgaacggaa atacaaagtc aaattgactt tgattccttt gatcgaacaa
    31741 tttgcagcga cagcgattac tgatcgataa aaattctatt tgtcataaaa ggctgggatt
    31801 taaaatccca gcctttttag tcatataata atttctacag caagtaaaaa gaaattcctt
    31861 ctaacatcag gctcaaatat cagcttgacc tgtccaacaa atcacctatg atcctacaat
    31921 gttaggcgac ttctcttcta cctgaagata tcttggagtt gcctgatttc ttaagatgcc
    31981 taaccttaac tataaaaaat tctaataata aacatatttt ctgatag
//
LOCUS       DASJON010000009        29403 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJON010000009 DASJON010000000
VERSION     DASJON010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.009"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(159..446)
                     /locus_tag="U3989_000311"
     CDS             complement(159..446)
                     /locus_tag="U3989_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713784.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            580..1248
                     /locus_tag="U3989_000312"
     CDS             580..1248
                     /locus_tag="U3989_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1713785.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(1539..2279)
                     /locus_tag="U3989_000313"
     CDS             complement(1539..2279)
                     /locus_tag="U3989_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713786.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(2409..3356)
                     /locus_tag="U3989_000314"
     CDS             complement(2409..3356)
                     /locus_tag="U3989_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1713787.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(3485..4249)
                     /locus_tag="U3989_000315"
     CDS             complement(3485..4249)
                     /locus_tag="U3989_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1713788.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(4253..4642)
                     /locus_tag="U3989_000316"
     CDS             complement(4253..4642)
                     /locus_tag="U3989_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713789.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            4777..5319
                     /locus_tag="U3989_000317"
     CDS             4777..5319
                     /locus_tag="U3989_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN1713790.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            5467..6099
                     /locus_tag="U3989_000318"
     CDS             5467..6099
                     /locus_tag="U3989_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713791.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6233..6358)
                     /locus_tag="U3989_000319"
     CDS             complement(6233..6358)
                     /locus_tag="U3989_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1713792.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(6492..7385)
                     /locus_tag="U3989_000320"
     CDS             complement(6492..7385)
                     /locus_tag="U3989_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN1713793.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(7567..8493)
                     /locus_tag="U3989_000321"
     CDS             complement(7567..8493)
                     /locus_tag="U3989_000321"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN1713794.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(8578..9339)
                     /gene="pflA"
                     /locus_tag="U3989_000322"
     CDS             complement(8578..9339)
                     /gene="pflA"
                     /locus_tag="U3989_000322"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN1713795.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(9583..11814)
                     /gene="pflB"
                     /locus_tag="U3989_000323"
     CDS             complement(9583..11814)
                     /gene="pflB"
                     /locus_tag="U3989_000323"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN1713796.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(12091..14541)
                     /gene="parC"
                     /locus_tag="U3989_000324"
     CDS             complement(12091..14541)
                     /gene="parC"
                     /locus_tag="U3989_000324"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN1713797.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(14553..16598)
                     /gene="parE"
                     /locus_tag="U3989_000325"
     CDS             complement(14553..16598)
                     /gene="parE"
                     /locus_tag="U3989_000325"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN1713798.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(16764..17198)
                     /locus_tag="U3989_000326"
     CDS             complement(16764..17198)
                     /locus_tag="U3989_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN1713799.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            17325..17963
                     /gene="plsY"
                     /locus_tag="U3989_000327"
     CDS             17325..17963
                     /gene="plsY"
                     /locus_tag="U3989_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN1713800.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(18095..18970)
                     /locus_tag="U3989_000328"
     CDS             complement(18095..18970)
                     /locus_tag="U3989_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1713801.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(19052..19843)
                     /gene="codY"
                     /locus_tag="U3989_000329"
     CDS             complement(19052..19843)
                     /gene="codY"
                     /locus_tag="U3989_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN1713802.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(19861..21261)
                     /gene="hslU"
                     /locus_tag="U3989_000330"
     CDS             complement(19861..21261)
                     /gene="hslU"
                     /locus_tag="U3989_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN1713803.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(21275..21823)
                     /gene="hslV"
                     /locus_tag="U3989_000331"
     CDS             complement(21275..21823)
                     /gene="hslV"
                     /locus_tag="U3989_000331"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN1713804.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(21860..22765)
                     /gene="xerC"
                     /locus_tag="U3989_000332"
     CDS             complement(21860..22765)
                     /gene="xerC"
                     /locus_tag="U3989_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN1713805.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(22963..24339)
                     /gene="trmFO"
                     /locus_tag="U3989_000333"
     CDS             complement(22963..24339)
                     /gene="trmFO"
                     /locus_tag="U3989_000333"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN1713806.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(24308..26386)
                     /gene="topA"
                     /locus_tag="U3989_000334"
     CDS             complement(24308..26386)
                     /gene="topA"
                     /locus_tag="U3989_000334"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN1713807.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(26508..27365)
                     /gene="dprA"
                     /locus_tag="U3989_000335"
     CDS             complement(26508..27365)
                     /gene="dprA"
                     /locus_tag="U3989_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN1713808.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(27421..28188)
                     /locus_tag="U3989_000336"
     CDS             complement(27421..28188)
                     /locus_tag="U3989_000336"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN1713809.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(28181..29041)
                     /gene="ylqF"
                     /locus_tag="U3989_000337"
     CDS             complement(28181..29041)
                     /gene="ylqF"
                     /locus_tag="U3989_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN1713810.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         7870 a         6191 c         5026 g        10316 t
ORIGIN      
        1 taataaaaag aaacagccaa taaagtagaa atctcgtggg ggagaacacc tttattggcc
       61 tgttcaacga atattatact gtatcatgac acttttttgc caaaaatgta aaaaatggtt
      121 gttttgacat cataaacgtc ttgttttctt atttttactt atatcaccac tttattttct
      181 atctctttca ctccataaag aggaatgagt tcttcttttg actgataata aacagctttc
      241 tcacggttct tcatcaaaaa aagaaagatg tctttcatag caagtggtgc tttgccgtgt
      301 tcaaataaaa agataaattg atttgcttta tttatctgga ccattgtcac aggcaccagt
      361 ttttgttctt gagccaaaaa aactccttga tactctgttt ttttgggtga ttttgtaaga
      421 attgtcaata agtctgctgc tcgcattttt ccactcctta tttgataaat aaaaagaaac
      481 agccaaaaat aatttttttt cattttcctc ttgaagtaag agtataagtc agtataataa
      541 agaagttgct gaaaattttc aaaagaaaag aggctagaaa tgaaaaaatt aaatttattt
      601 acagaattgt gcggaatcat tttaggcgca ggaatctacg gattagctgt gacaggaatc
      661 aatattccat ctaagctggc agatggtggg gttactggta ttgctttgct tttgcataat
      721 ctttttggat tcgctccttc tgtcacttca ctgcttttta atttaccttt attgcttatc
      781 tcattgtttc tttttggaaa acacgctttt ctccggacga tcgtcggaac atttgcgcta
      841 gttttctttt tacatttatg ggagagcctt catatacatt ttgctgtaga tagtctactg
      901 atcaatagtt tgatgacagg tattctttct ggcattggtt gcggtctcgt ctttcgattc
      961 ggcggaagca caggcggaac agatatcatt tatcaagcca ttgaaaaata ttttcatatt
     1021 aagattggca aaagtctttt cttgattact tttggtattt tagttgtctc cttgctttat
     1081 ttggatctca cacattttct gtatacatta cttagctgtt ctattctatc ttatacattg
     1141 aataaagtga agtatctgga aattggatct gttttgcccc aattggaagc aaaacagact
     1201 acagaaacat ttgaaccact tgatcctctt gaacagttag agcactgatt cttatttttc
     1261 actactttat ttaatttaca gaaatatcat tatgtaaact aaaatgatat tattcagtaa
     1321 aatcttcttt ataaaaaaat aaactatttt tgcaaacgat ctgcgaaaat agtttgtttt
     1381 tttattttgc cattattttc ttacttattg ggagaaaaaa ttcgcttaaa ctcaaaaaaa
     1441 tttgaggata cgatataaac tttcggatag agtataaaat aaggaaacgc cgcagcgttt
     1501 cctttccaac tgtagccgaa tataaatttg tatatcactt atttaaaata gctattttct
     1561 cccaaagagt ttccttgatc atcgatcaat ccttgtaatg agcgttctaa gataccatgg
     1621 ctttttttcg attttttttc ttctgactgc tttgtttcct ctaacgtttt tgttcgattg
     1681 aatgttcgta cagacggctc accatttctt ttttcttgat acggcgtcgg ttctgtatcg
     1741 aacaacagat aagagtcatc cgtttttttc atcgattcga ttaaatgatc ctcagaaatt
     1801 ttgctttctc tagggtctgg gataatcgat gcgggtacat atttaggaac aaaataagaa
     1861 cgctcccgca ccttcgtgtc aacagatgcg tttcgtgtag tatgcttgga ctctgtcctt
     1921 gtttcacttt gtctgacaga acgagaggaa tcaccaaaaa gtttttttcg tcctgtcgat
     1981 gtgatttctg attggtaatg gccggtataa tccggtaatt ttcctttatg cttttccaaa
     2041 ttttccttac gcggagctga cttcaatcca tttgttgcac gacgcttgcg acggattttc
     2101 tcggtgttcg tgttcgtgaa atgaaattct ttcatctgtt caaacgggga ctgattctgt
     2161 tcagtcaaaa gatcttcttg tccgtcaaat aatactcgct tcttgtcgtt ggttaatttt
     2221 acgcctaact catcatcgta cgcaggaaaa cggaaatgtt ttcgtctgat ttctttcatt
     2281 caaaccatcc ttctcaaaaa tggaactttt ctcaataata gcacagattc attgttttca
     2341 gtagcaatgc gctgattatt ttaagaaaat agcgatgaat ttcataaata tatatatagt
     2401 aataagactt aattcgttgt ctctctgata actagttcag gaaaaaaacg acgataacct
     2461 tgactcgccc ctttttcttc tatttttttt aacaacattt ctccacatac ttcacccata
     2521 cgtataatat cttgcttgat cgttgtcaat ttaggtgaac tgatttggtc taaaaatacc
     2581 ccatcaaatc caatcacacc aaattcttcg ggcaagtgtc ctccagcatt tataatccct
     2641 cttactgctc ctagtgctag ccgatctgaa ctacatacaa aacaagtttt atctgtaaaa
     2701 tctgcccagt tttctgtcat atatctttct acttctgtcg aatgatttgg aaaccgcaag
     2761 attttactag gaagatcaac ttccttcatc acagataaat aaccagtttc ccttgaaatt
     2821 tcaaaaggct cgtccacttc gataccgata taaataatcg tttgataatc tctttctaag
     2881 gcatattgcg ttgcttttgc tgtaccatat tcattatcgc tgtctacata atcatatcct
     2941 tcagaatttt ccccaaacaa gacaactggt ttttccaaac ggtcgatcca atcgaagtct
     3001 ttttttctga cacctgtgat gatgtatcca tcacaagaac cgacgtcgaa ggcatcattt
     3061 gtaactaatt ggagagaata atgcgcttta ccgatccgtt tagcgattcc catcagcaaa
     3121 ttcatgtaat agggctcagt tgtgtcgatt tcttccagta taaataattt gatgatctgc
     3181 gaccgattcg tgacaagtgc ttttgcagca atatttggac ggtaatccag ttcagccatt
     3241 gctgagaaaa ctaactcttt caattcgtca gtcacctgtt ccggatgatt gatgactcga
     3301 gagaccgtca tttttgaaac attggcacgt tttgcaacat ctgctaaagt gggcatgctc
     3361 tcttcctttc taacatgttg tcattcttac tatcaattat accaaatttt cctaacagag
     3421 tttattatag cccaaaataa aaaacggaaa atcctctatc gtcatgagtg attttccgtt
     3481 tcaattattt atactttttt atcaaatttt ttgctaaaaa agtggaagct tcttccatta
     3541 gtggtatttg taccatatgt cgctgtgttt ttgaggaacg aaaaacaatt tcttttccgt
     3601 aagacttatg ccgatttttt tctacaaatc gttccgtttg tatataaggg attttttcgt
     3661 cagcttgtcc atgccaaaag aacagaggac gtccagctaa cttttccggc tgcaatgaca
     3721 aatcatagct cttaacccat gaaatcaaat caaaatagtc ttttggtacg acataattat
     3781 attccgatgc gcgccttacg atttcttttc catattccaa aggtgcaggg gaacccatca
     3841 aacacacgcc cgctactata tgaggatgtt tagccaacaa ggcgcatgtt gtcattcccc
     3901 ccatcgaaac cccgccaact gcaagctgat catgtagaag acctcttttt tctaaatgct
     3961 tgataagtgt gtcaaattca aacagatttc cgtagatact atcccaaaat gtataggatg
     4021 gaatagaaga aaccggctgt ttgcgctccc catgattcat cgcatctggc aggatcacac
     4081 gaatgccctg ctgcgcaatt tttcttcctt gcgtcaaaac taactcctta cttgtctgcc
     4141 atccgtgata atagatgaca acgggaatac gttcctctct taattctttc agaacaactt
     4201 ctaacactgg aatatgatgg acagaacgtt tcaatataat tggattcaat tgttactcac
     4261 ctattttcat taaatttcct gatcgccata gcaaccatgc tccaacagtt gtctggaaca
     4321 ggttcagtgt accaaataat ataaatgcag gactgaatgg ctcgggaaat aagcacagag
     4381 aacaaagtcc caccactcct aatgctagcg aaaggagtgg ataaatagaa aaattcaaaa
     4441 aactggcaac gctaccgaaa atattaccaa taaacagtat caaaaagaaa aaaacgctta
     4501 aaattaaacc atctgtcagc gacatctgaa aaacaatgtt ctctacctca attggctgtt
     4561 gaatcgaata gacacctaag tatatactga gaaggagact tagcacgtgg ctggcacaaa
     4621 acacacgatt gaccgttttc attttaatca cctaacctat ctttataatt ctattataat
     4681 cattttatca tttttttgta tgtataaagt ttcaattttt cttcacttac gatttttttt
     4741 gatattgttg ttatataaat gaaaggagtt ttacaaatga ttgatccaaa aatgttgggg
     4801 atgatcttcc tcattaattt tgcttatatc actctgaaca cgttacgttt catgttgaca
     4861 atgaaaggct atcgactgat tgctccactt gtcagtatgg tcgagatcac gatttatatc
     4921 gtcggcttat cccttgtttt agatcgttta gataatccac tgaatttatt tgtctatgcg
     4981 ttaggttacg cagtaggtat cagtgtcgga attttgattg aagacaaatt ggcattagga
     5041 tacatcatgg tcacaacgat tttgccagca aatagcgcag aagataaaag tttgccgggt
     5101 atactgagag aacacggtta tggtgtgaca cagacttctg caatggggct ggaaggtgag
     5161 cggctagttc tcgaaatact ttctcctaga aaaagcgaac gtgaactgta tcgcctgatc
     5221 aaagaaatcg aagagcgtgc ttttatcatt tcttatgagc caaaatatat ttcaggtggt
     5281 ttctggacta aaaaagtgag aaaacgacaa ggaaaataac tcacaaactt aaaaactgtc
     5341 taatatctaa attaaatttt aaatgttaga cagtttttat ttattatttt gattaaaaaa
     5401 catcgacata tactcatcaa tttgttatta tactttatat acttatcaat ttaatttgga
     5461 gatattatgg aaattcaacg tagaaccact aattttttac atttaggctc tttttttgca
     5521 actttgacga tcttattttt tctatgttat tcatacctta agacctcaca tgcgccttct
     5581 agtgcattag gctggttgct tttggatcat tctgatatca cagaagccga agaaaatccg
     5641 ttttttttga ttttgtcttc tctttgcggc ttactgtttt ctgtttattt cggtgcctat
     5701 ttttttcatc gacatcctgg tacgttaaac gaagcttcaa gcgtattgat tgcatttaaa
     5761 acgttttctt ttttagcaat tcttttttat tcgtttaatc acaatacgct tttcttcttt
     5821 atcatgaatg gtttagtcgt gattttatcc atttttactt attttatttt ttcactcatt
     5881 tttagccaat tgaaatgtcc gctcttcttt cgattagttc ctgtcacctg ttttatttac
     5941 agcttgtttt ccttttttgt ccttgcttta atgccactga ccaacccttc gacgatccaa
     6001 gtcgcaggaa atctgttgga tatgctcttc atcgtcagtc taggtgtatt tatgtggcaa
     6061 agaaaaaaca aacaaaataa aaagcatttt gaagcctgac tattacgact tcataaatgc
     6121 ttttttagtt ttaaacactt caatctaaaa gaataaccca agattggata aattattaat
     6181 gactcgccat tcgtttctta gtgattctgg attatcaaat ggacttccta atctaatcag
     6241 ctagttcttc ccataaaatg ccatacttga ctgaatcata atattgatta tttaaaaatc
     6301 taacttttct tatcactact tcctttgtca ttccacattt ctcaccattt ttttgcattc
     6361 ctttgtttcc agaccatgta gtgaatctaa taggtggtag ataggcaaaa tactcaaata
     6421 attcttttaa ccataaagga agagaaaggc tcctcctgca agagtcagta taatttttgc
     6481 ttactgctct cttaggtttt actcactaaa actgttccat attctttgat ataagaaaat
     6541 gcatgcggta taaatttctt gctcagctca gaaaagattt gtcgtttaag aaaggctatt
     6601 tcctccattg tcaatgaagg atccaatgtt ggtaattcaa tctcaaaatt gtttttttcg
     6661 acaataggta attctaatgt gaatgattct tcttccttta tagtattcgt cacaagtaga
     6721 gactgatgga aaatactagg ccggcgattt ttatatttat tcttacgtat cctgtcaacg
     6781 ataacttcta tttgatcaaa aaagtacgat tcatttacgt ctccaataag ttcggctaat
     6841 tctttgaatg ccttcgttac aggaagctga ttcttctgta caaaaaagta attagccaaa
     6901 gtaagcggga agtgttctat agaggaaagt gtgataaaac ggtacttctc atttcctgca
     6961 gttgtcctca gcaattctgt ctctcggctg tttcttactc cataaaaata cgcttctttc
     7021 cgttcatctt cgaaacaata cgcccaaata acttctccca tcgctaattt ttgttcatca
     7081 acagagagtc tcttcaattg ttcagctatg gtttcagcag ccgaagtggc ttgttgcaag
     7141 taatctttag gatcaaatat tggaaagttc aaatgatatc ttcgttcttc cctcttgatt
     7201 aatcctgcat cgatcaataa atcaagaaaa tgggaaaatt tattttgcga aaattctttt
     7261 ttcaattctc ttagaatcgt ttcttgttct cgatgttcat atagataatc ggtcaatgaa
     7321 gaaaaaagcg gatcatcggt cagtttttga taaaagtttt ccttattttt tgaataatag
     7381 ttcatatatg ttctcttttc tatccgtttt attttactta aaaggctttt acgtaaaaag
     7441 gaggaagaac aagagtcata attgactgtt attctcctcc atttcttcgg cctttagtat
     7501 gttctatgtt tctttttata tcaatcgctt atccattcat tttgaatgct tcatactacc
     7561 atttgattag ctaaatacag ccgtcaattg aggtacgact tgttttttac gtgaaacgac
     7621 acctttaagc aaagcacggt tgttggtcaa agttgtttgg aaagcttctt ctactttttc
     7681 ttttccttta ccagcaacaa gtaattctga atcactgtct aagatgtttg tgacaactaa
     7741 tacgaacaga tcgtagccat ttgctttcat ttcatcttcc attgctgctt ctagctctgc
     7801 ttgtcgatcc atcacttcat taaggtccac tgtattgact tgagcgatac gaacgttgga
     7861 actgcccatt gggaaacttt ttgcatccaa atcaagaaga accgcagctg atttatcact
     7921 tagatttgtt cctgctttca acatttcaag tccataagtg tttagatcga cttcagcgat
     7981 tgctgcaagt tcttttgcag ctgcttcatc ttcaggcgta catgttggtg atttgaacaa
     8041 caatgtatct gaaatgatag ctgagagcat gattcctgcg atttctttag gaattgttac
     8101 ttggttttct ttatatagtt ttaaaataat cgtacttgta catccaactg gttctgcacg
     8161 atagtacaat ggattagctg tttcaaagtt tgcaatacga tggtggtcta ctacagctaa
     8221 aatatttact tcgctgatat ctgaaatact ttgttggaat tcattatgat ccacaagcat
     8281 gacatctgca ttttctgctt tagctgactc tactactcgt ggtgcagata gctgaaagtg
     8341 gtctaatgcg tattgtgttt cttcattagg tgtacctaga gcaactgctt ctgtttcttt
     8401 acctaattca ttttgcaagt gtgaaaacgc aatggctgcc ccgattgcat ctgtatctgg
     8461 attttggtga ccaaaaacta aaatttttga catgtcattc gctcctttgt gatttctcct
     8521 atttaataat agcaaaaaaa cggaaagaag caatacttat cttctttccg atgcgaccta
     8581 tcgcgttaaa tattttgtat actcttctgt atgaagcaat cgtttagcat tttcgacacg
     8641 ttcttttgta ggtggttcga tcccttctaa tggatatttc aaaccaagtg tttcccactt
     8701 gtattttccc atcgtatgat aaggaagaat ctctactctg tctacatttg acaaggtttg
     8761 aataaagttg tctagtcgga tcaaatattc atcataatca cttcgctcag gaactagtac
     8821 atggcgaatc caaaccggtt tgttgatttt tgacaaatat tgagccattt ctaaaatatt
     8881 ttcattgtcc caatgcgtta gttttttatg ctcttcatta tcgatgtgtt tgatgtcaaa
     8941 taacagtaaa tctgtatact tcatcaattc ttgaaaacgt gaaaagaaag gttcttcata
     9001 cgtaaaaggt tttccgcatg tatccaatgt agtatgcatc ttccgctctt ttgctttttt
     9061 aaatagttcg atcaaaaaat cgatctgcaa tagaggttct ccaccactaa cagtaatacc
     9121 acctttatct ccccagtaag gtctgaagcg ttctgcttta tctaaaagtt catctgctgt
     9181 gtattctttc cctccgccaa tattccaagt atctggattg tgacaaaatt ggcaacgcat
     9241 gcggcatcct tgcataaaca cgacaaagcg cagtccaggt ccgtctactg aaccgaaagt
     9301 ttcgatagaa tgaacatagc cgattgtttt ttcttccatt taagttcaca tcttttcttt
     9361 gatttcattt taagtacaag ttagctgtcg aagcgaagct ttctgaacaa aatttttcgc
     9421 aattgccgct tcgttatgtg taaaatttac agggacggaa catacaagtc ctgcccctgt
     9481 aaatcgtttc tttcaaataa gcatactgat tattcgattt tgatgcgatg gctattcaac
     9541 gtcctttcga cgctaagtcg aaaaactttt ttcacgaact ttttacattc tgtcgtgtga
     9601 agttctcatc aagacatcta attgttgttc tcttgtcaag tcacggaatt taactgcata
     9661 accagatacc cggatcgtca agttcggata tttcattgga ttttccattg catcacgcaa
     9721 taaatcatta ctgaatacat tgacattcaa atgataagct ccttttgaga agtatccatc
     9781 taagacatta cgcaagtttt cgattcttgt atcttgttct tttccaagac ctgaaggatt
     9841 gattgtttgg gtatttgaaa ttccatccaa tgcacagccg taattcattt tagctgttga
     9901 gttcaggcta gctaataacc cactttgttc cgcattgtaa cttggatttg ctcctggcgc
     9961 aagtggtttt ccagcttcac ggccatcagg agtatttcct gtatttttac cgtaaacaac
    10021 atttgaagta atcgtcaata atgaagttgt tggttttgag ttgcggtaag ttttatgacg
    10081 tttgatttgt cccatgaagt attccaagat ccatgaaact aattcatctg cacggtcatc
    10141 atcatttccg tatttaggga agtcgccatc cacttcaaaa tcaactgcta ttcctttttc
    10201 attacggatt gcttttactt ttgcatattt gatcgctgac aagctgtctg ccgcatgaga
    10261 gatcccagca atacctgtag caaatgtgcg ttgcaagtca gaatccatca aagccagctg
    10321 agctgcttca taagaatatt tatcatgcat ataatgaatg atgttcaacg tattgacata
    10381 tagttctgct aaccaatcca tcatattcat gaagttatgt ttcacttctt caaagtctag
    10441 tatttctgat gtgattggac gatattctgg tccaacttgg gcgccagtca cttcatcgac
    10501 tccgccattg atggcatata atagtgtttt agccaagtta gcacgagcgc caaagaattg
    10561 catgtctttt ccgacaactg ttcctgatac acagcaagca atcgcacaat catcagaacc
    10621 ccattgttca cgaagctgat tatcattttc gaattgaatc gaagaacttt cgatagcgat
    10681 tctgctagca tatgttctga atccttctgg aaggtgttca gaatacaaaa cagtcaagtt
    10741 tggttctggt gaaggtccca tatttgttaa tgtatgaagc agacggaaat cattctttgt
    10801 aactaatgaa cgaccatcta atcccatacc tgcaatggat aaagtagccc agatagggaa
    10861 tcctgagaat aattggttat attcaggtgt acgcgcgaat ttgaccatac gtaatttcat
    10921 tactaagtgg tcgatcaatt cttgtgcttc tttttctgtg attactccat tatccaaatc
    10981 acgttggata tagatatcaa ggaatgctga gatacgtccg attgacatcg cagcaccatt
    11041 ttgtgatttg attgctgcta aatatccgaa gtatagccat tggacagctt cttttgcatt
    11101 tgcagctggt ttagaaatat catagccata tttcgcagcc atttctttca tttgcgctaa
    11161 tgcacgatat tgttcagtga tttcttcacg taaacgaatc acttcttcac tcattgtatg
    11221 ataaccagtc atatcatgat cttttttctt ttgttccatc aaatagtcga ttccatacaa
    11281 tgccacacga cgatagtcac cgatgatacg tccgcgaccg taagcgtctg gaagccctgt
    11341 aatgattttg tttttacgag cagcacgcat ttcagcagta taagcgtcaa atacaccttg
    11401 attatgtgtt ttgcgccagt cagtgaaaat atgtgtcaat tcatcgtttg gtgtatagcc
    11461 attgcttttt agcgcattgt tagccatatt gattccgccg aacggcatga atgcttgttt
    11521 caaaggtttg tctgtctgta gaccgacgat tttttccaaa tctttatcca aatagcctgc
    11581 atcgtgtgaa gtaatagttg atacgatttc tgtgtccata tcatagacac cgttacgctc
    11641 gaattggata tcatttaact tttgcaattc atcccataat ttttctgttg cttctgttgg
    11701 gccttccaaa aagctgtcat caccgcgata ttcagtgtag ttattttgaa taaagtctct
    11761 agtgtctacg ccttctttcc atttggtacc tttaaatcca ttccattgct ccattgtatg
    11821 gacctcctaa atttctgttt tatgtaacag tttttacgtg attagtataa cacagcaccc
    11881 tatctttgca agggttttct tgttttttat ttaacaattt ttatgtttta tttacatata
    11941 tttaaatcaa cgttgacata ccaacattta agcattgtga acactttcat ctttttttgc
    12001 aaacgtataa ggatttatta aaaatgaatg aaaaagagac gccacctgta ttaatacaga
    12061 tggcgtctct tcattaaaat actacaacag ttacgactcg atgaaaatct caggagcatc
    12121 gataacttcg atgacttctc cacctttttt ctcatccatc gcaaatgatc cattcgatgt
    12181 tcgatcacct atagggaaat cagttgggtc aatcgttaac tgctgccctt tttgattcat
    12241 caaggttatt tcttttgatc ttccttccga catatagata atacggtgcg gatttttttt
    12301 cagctcacgc atgaccgtca ctccccgctt cgcacgtgaa gtttggctga tttcttgagc
    12361 aagcatgcgt ttaacagctc ctcgctgagt aacgattacg atcggcgtat caccttctgc
    12421 taacacaagc agcccgttga taatcctatc tccatctttc aaattcatag acttcactcc
    12481 agctgccttt gctccaacaa cgggaacatc tgttaaagga tagcgcaaac ccattccttg
    12541 atgcgttact aagaaaacat ctaagcctgg ctgatcttct ttcagataca cagcaatcag
    12601 ttcatcttct tccgccttta gcttcataca gctgagcgga cggctcttat aggtgcgcca
    12661 tggttcgaac tctgtcattc gtgtttgctt gatcaatcca ttcttgctga tgaatacaaa
    12721 tgttttctct gcttgaattt tttgatatgg gaaaacagct aaaatcgatt cgtctacaga
    12781 cagattggag attgtttgag aaatgtgctc accagcatct ttccatttca aatcgggtag
    12841 ttcatggaca gggcggtaga tcacatttgc tttatttgtg atcagaagaa catggtccaa
    12901 tgtactcaat tcacctgagt atagcaagta gtcgccttct cgcatcccga tttcttctgg
    12961 tttggaagca ctataagaac gaaggcttgt tcgcttcaca tagccttctc gcgtcacact
    13021 gacaattacg tcttcttgtg ccactaagac ttgcgtatca attttgattt cttcgatttc
    13081 gtcttcgatt gtcgttagac gagcggaaga ataatttttt ttcacttctc gcaattcttt
    13141 tttcataaca gagaaaagct ctttgtcatt tgaaaggatt ttattcaact ctgtgatttg
    13201 cgcaatcaag ctttcggatt ccttttgaag ctctgtgata tccgtgttcg tcaaacggta
    13261 taattgcaat gtgacaattg cttctgcttg ttcttctgta aattgaaaga cgtcgactaa
    13321 attctttttg gcgtcttttt tgtctttact ttcacgaatc gttgcgatta cttcatccag
    13381 aatcgataaa gctttcatca atccttctac gatatgctgt cttttttgag ctttggctaa
    13441 atcgaattga ctacgttttg ttatgacttg ctttcgatgc tcgatataac ttcttaaaat
    13501 gtctttcagt ccaacttgat gcggagtcat atggtcgata gcgaccatat tgaaattgta
    13561 attgatctgt agctcagtgt ttttgaacaa ataattcaaa attccttgag cattggcgtc
    13621 ttttttcaat tcgacgacaa tttgcagacc tgttctatca ctttcatctc gtacttcagc
    13681 tattccatcg attttcttat tcaaacggat ctcatccatt tttttcacca aggtcgcttt
    13741 atttacttca tatgggattt ctgtgatcac gatttgctgt ttattaccct tcattgcctc
    13801 tatctttgtt ttcgaacgaa gaataacttt tcctcgtcct gtttcataag cttttttgat
    13861 ttcatctttt ccttgcaaga tccctcctgt tggaaagtca ggtccaggaa tatattccat
    13921 caatttttca aggctcgctt gtggatgatc gatcatatag attgtgccat cgatcacttc
    13981 agctaagtta tgagtcggga tttctgttgc atatccagca gatatacctg tcgatccatt
    14041 gacgagaagg tttgggtatc gagctggaag gactgttggt tctttttctg tatcatcaaa
    14101 attccacacg agatcgactg tttctttctc gatatctgct agcatctctc cacttaactt
    14161 ggataatctg gcttcagtat aacgcatcgc agcaggtgga tcaccgtcca tactcccgtt
    14221 attcccgtgc atttcgatca gtacttcccg caacttccag tcttggctca tgcggaccat
    14281 cgcttcataa atactgatgt caccatgagg atgataattc cccatgatat ttccgacaga
    14341 ttttgctgat ttacggaatc ctttgtcata cgtattgcca tctttgttca tggcaaataa
    14401 tatccggcgt tgcactggct tcaatccatc acggatatca ggcagtgcac gttcttggat
    14461 aatatatttg gaataacgtc cgaagcggtc acccatcact tcttctaaag ttaattcttg
    14521 tacttcttgt cgattttcca tcaggtgacc tcctattcta cttcaaataa ctgattgttt
    14581 tcatttttat ctgctcgttc ttcatctaat aaatcattag aaactgaagg agagatttca
    14641 gtatcctctt ttttatctaa gatactgcca tcttcttcga gcgtaaattg cacgtgattt
    14701 tcgatccatt ttcttcgagg ttctactttg tcacccatca gtgtggttac tcgacgttca
    14761 gcttgcgcag catcatcgat acggacgcgg atcaacgtac gggaagtcgg atccatcgtt
    14821 gtttcccaca attgttccgc attcatctct ccaagtcctt tatatcgctg cagcatatat
    14881 cctttgccga cttttttaat cattgctgcc aattcgtcat ctgtccacgc atattcaatg
    14941 acttgcttct ttccttgtcc tttggatact ttatataaag gcggtaacgc gatatatact
    15001 ttcccagctt caatcagcgg tttcatataa cgataaaaga aagtaagcag taatacttga
    15061 atatgggcgc cgtctgtatc cgcatcggtc atgatgatta ctttgtcgta attacagtct
    15121 tcgatagaaa actcaggacc gactcctgca ccaatcgtat agatcatcgt attgatttct
    15181 tcatttttca agatatcttg cattttagct ttttcggtat tgatgacttt tcctcgtaaa
    15241 ggtagaattg cttggaactt tctgtctctt ccttgtttag cagatccgcc agcagaatcc
    15301 ccttcgacta aatacaattc atttttctta gggtttcggg attgtgcggg tgtcaatttt
    15361 cctgaaagaa gagattcgcc ttttttacgc ttcttcccat ttcggctttc ttctctggct
    15421 ttacgtgctg cttctctcgc ttctctggct ttgattgctt tacggaccaa catctggctc
    15481 atttcgctat tttcctgcaa ataaaagccc atttgttcac tgatgacgtt atctactacg
    15541 gtacgagcaa ctggcgtacc aagtttttct tttgtttgcc cttcaaattg cagaagattt
    15601 tcaggtacac ggatagaaag tacagcagct aacccttctc tgaaatcact accttccaga
    15661 ttcttgtccc gttctttcag caaaccaact tttctcgcat attcattata agatttcgtc
    15721 atcgccgctt tcataccagc ttcatgggtt cccccatctt ttgtccgaac gttattgaca
    15781 aaagataaaa cattttctga ataaccgtca ttgtattgat aagcgacctc tacttcgata
    15841 ccttcttttt ctcctgaaaa ataaacaact ggtgtcaatg tatctttttc ttcattcaga
    15901 taatcaacaa attctttgat tccttcttcg taatgaaaaa tttccttgac tggttcttct
    15961 ccacgaagat cagaaagaga gattttaacg ccttttagaa gaaaggcaga ttctcttaac
    16021 cgttcggcta aaatttcata tgaaaatttc gttgtactga atattgtatc atctggtaaa
    16081 aaatggacag tcgtcccgtt tgcttttttt gtttttccta tctttttcaa agtaccgtca
    16141 ggtttaccgc cgtttttaaa tttctgctga tactctacgc catctctaac gatcgttaca
    16201 attagccatt tcgataaggc atttaccaca ctggctccta caccgtgcag tccaccagaa
    16261 gttttgtacc ctccttgacc aaactttccg ccagcatgca gcacagtaaa aatcacttca
    16321 actgttggta ttccggaagc atgcattcct acaggcatcc cacgtccgct atctgcaaca
    16381 gttatactat tgtcttcatg gatggtcaca tcaatctcac tgccataacc tgataatgct
    16441 tcgtcaactg cattatcgac gatttcatag actaaatgat gcaagcctcg gctgtcagtg
    16501 gatccaatgt acatccctgg tctttttcta actgcctcta atccttccaa tacttggatg
    16561 gacgagtcat tgtattcgtt gtttactttt tttgccaagg caaacgctcc ttcaataata
    16621 ctttgcggca aaaccgcacc acgattatca tactagaaaa ggtcgtgaaa aaaaagaggg
    16681 tagacgagaa atcaatttta attatctcct aaccgctttt tcgacattcc cctctatcga
    16741 tcatctcttc tttgtggtca tttttatttt tggttttctt tcaacttacc aagttcaatc
    16801 ttaatacaac gatccattac gatatctgta cgtcctgctt ttcttagcat ctcagctgct
    16861 tctttgtttt caatccctaa ttgtgcccag aacactttag catcagtttt caaaaaatca
    16921 cctgctattt caggtaaaaa ttcactccgt ctaaatacat ctacgatatc gattggatca
    16981 tcgatttctg tcaaattcgc atagactttc tcgcctagaa tttcttcacc agccaataat
    17041 ggatttactg gtatgatttt atatccattg tcttgcaata cctgtgcaac catgtaactt
    17101 gttcgttctg gtttattgct caagcctacg acggcaatat tttttgcttc ttccaaatag
    17161 ttgaaaatcg tttcttgtgt tggatttttt acagacattt ctgccacccc ttgcttttct
    17221 ttttattcca tctattatac ttgtatcttt cattttttca attgttcctg ttacctattc
    17281 atgctaaaat aaaggatagt tttcaacttg aaagagggta aaaaatgaaa ttgatcttat
    17341 tacttatttt agcatatctt ttaggttcta ttccttccgg tgtctggatt ggcaaattgt
    17401 ttttcaaaaa agatatccga caacacggga gcggtaatac tggaacgacc aatacatttc
    17461 gtgtattagg caaaacagcc gggattgttg tcctgcttat ggatatttta aaaggtacat
    17521 tggctacttg tttgccgatg atcttccacc tttctgggat taaccctctt tggtttgggg
    17581 tatgcgccat tcttggccat acatttccga tttttgctgg gttcaaagga ggaaaagctg
    17641 ttgcgacaag tgcaggaatg cttttaggat ttaatcctgt attttttgtt tattcttgta
    17701 tcatctttat cgttagttta ttttgtacaa gtatggtgag tttgagcagt atgatcagtg
    17761 cagtattgat cactttatct acgatcgtcc ttccttatgt agctcctgtg attttggcta
    17821 agcccaattg gttactgacg atcattgcct ttttagtttc ttcctttatc ttttatcggc
    17881 ataaagacaa tatcaagcgt atcagaaacc atactgaaag tcgaatcccg tttggtttga
    17941 atagcagcaa gaaaaaatcc tgatactttt tcattccaaa gagataaaac gggagtgtga
    18001 caaatcgatg tcacactccc ttattactta caggatagct tttttacaca ccttggtaaa
    18061 cggtgttccc tgctaacttc tcggtcacaa cctcttactt cactgtgatg gaatattttg
    18121 tcttgaagat ttcatgggca cctagttgat tgatccctac tttttccatc aagtttccac
    18181 tactgttcac cgtgtcagcg attccgcacc acggttcgat acaaacgaaa ggcgcttctt
    18241 ttggataagg agaccagatc cctacataag gcatatcttt atagcttaaa gttacgctat
    18301 gggaagattt ttcacttcga atggcgtacg catttagacc ccttgtttca aaaaccaagg
    18361 catcttgctc aaaaagttca cgattcaaag ccagattcgt atttgtctga cctaacgtct
    18421 tttgatccat atcgatgaat gggcctttca atggtaggcg taatcttgat ttcaatggag
    18481 aaaatgccag atagaaatct tcaaatgaca atccttcttc aagaggtaca ttgaaagcag
    18541 gatgtccacc gatagaaaag aacatttctt tttcaccggt atttgccact tgataatgaa
    18601 ccgtgatgcc gtctcctcct aattcatagc tgatcactaa ttcaaaatca aatggatact
    18661 tttctaatgt ctccggcgta ctccttaaga tgaaactagc catttcttgt ccatgttcaa
    18721 tgacttcaaa atccatatcc cgagcaaatc cgtgttgccc catttgatat gttttccctt
    18781 gataaacata ctgatcatct tttaaacgtc caacaaaagg gaataaaacc ggtgcatgtc
    18841 tgccccaata cgctggatca ccttgccaga tatactctaa accatgttct tttgatttca
    18901 gactgatcaa ttccgcaccg tgttcagcga tttcggcgat cagctggtca ttttgaataa
    18961 taacagtcat tgccattcct ccaacttcga cttacttttt aaaatgggaa ggctggaaca
    19021 aacatttcta tctgccccag tccccttact tctaataact gtgttttgct aactcgtctt
    19081 taaaacggga attcaatact tttaaataag ttcccttcat gcctaacgaa cgagattcaa
    19141 tgattccagc tgattccaat tttcgtaaag cattgacgat cacagaacga gtgatgccga
    19201 tactatcggc aatattagaa gcagtcagac gaccttcatc tccatccaat gcttcaaaga
    19261 tagcttgtac agccttcaat tcgctataag ataatgtgtt gattgccatt tggacagccg
    19321 ttgcactccg tacatctgct tcgatgcttc gagacttctg ataaaggatc tgcataccga
    19381 caaccgtagc tccatattct gctaagacta gatcgtcatc tgcaaacgat tgttccatac
    19441 gagataagat aatcgtaccc aaacgttcgc cagctccaaa gatcggaacg acagtcgtca
    19501 gaccaaatgg atatttctct cttgactcta ccggaaatgc agtaatatca ctagtaattg
    19561 ggatattagc ttcagttttc atcaactgtg ccactgtatc agtataagat tcaggaaatt
    19621 gtttctcaac aaacattgtt ttgattcgtt cattgttcac atcgtgacgt tcattataac
    19681 ctagtaatac tccgtcactt cctatgatat aggtgttgct gtcaagaata ccacctagtg
    19741 tgacagccat tttatcgtaa ggcaaatctg cttgcatgtc aaaagtattc ttttgctgca
    19801 aaagtgtgtt tatcttgcgg gttttcgtta gcaagtctgt cattgtcgtc ctccgatttt
    19861 ttataaaata tagcgactta aatcttcatt tgccgcaatt gagccaagtt tgtcatccac
    19921 atatgcttct gtgatcgtta tttctcccat ttgcatatct ggtgcttcaa ataatagatc
    19981 ttctagtagt ttttccaaaa tggtatgcag tctgcgtgcg ccgatattat ctgtgtcacg
    20041 gttgacatcg aaagcgattt gagcgatccg ttcaattgct tcttttgtaa acgtaactga
    20101 cacattttct gtaccgatca acgcgacgta ttgttttact aaagcattat ttggttcggt
    20161 cagaatcttc acaaaatctt cagctgtcaa atcatctaat tctacccgta tcgggaagcg
    20221 tccttgaagt tctggaatta gatcacttgg tttagacaaa tggaaagctc ctgaagcaat
    20281 gaataggacg tgatctgttt ggatgatccc gtattttgtg tttacttgtg aaccttcgac
    20341 aattggcaat atgtctcttt ggacgccttc tctggatact tctccagatt gctgtgattt
    20401 agaagtgatc ttatcgattt catcgataaa aataattcca ctttcttccg ctaatttgat
    20461 tgcctcacta tggatatcag catccttgac taattttgct gattcttcct tgatcaataa
    20521 ctctaaagct tctttgactg ttaccgtccg ttccactttt ttcttcggtg tcaaagcacc
    20581 tagagtttca tttaggtcga tccccatttg ctccaatcca ttgttcatca ttggtgcagg
    20641 tttcttttgg tcttcaactt caatggttac ttcacgacta tccagtaaac ccttttgtag
    20701 ttgttccaag actgtacggc gattgattcg aatatcttct gtcacttctt cttgcggttc
    20761 agcggtattt tgctgactag cattaaaaag attcatcatt tgttcaaatt gattagtgga
    20821 ttgtttctgt tcttttttga taccaggtac caagattttt accaaacgtt tttctgcatt
    20881 ttttttagca cttgtgtata cacggctgta ctgctctttt tctacgattt ggatcgcatt
    20941 ttctactaaa tcccgaacca ttgattctac atctcttccg acataaccga cttcagtaaa
    21001 ttttgttgct tcgactttga caaatggtgc attgacgatt tttgccaaac gtcgtgcgat
    21061 ttctgtttta ccgacccctg taggtccgat catcagcata ttttttggtg taatatcttg
    21121 ttgcattttt tcatctaact gcaaacggcg atagcggtta cgcaaagcaa cggcaacaga
    21181 tttttttgct gcgtgctgtc ctacgatata ttcatctaat tcacttacga tttgtcttgg
    21241 tgtttgtttt aattcataca tatctactca cctcttacag ttcttctacg ataatattat
    21301 gattagtaaa gatacagata tctgcggcaa tatttaacgc attttctgca atttctttcg
    21361 cactcatgtc gttttgagca tagtttttca ttgctcgggc tgcagccaac gcaaaattcc
    21421 ctcctgatcc gatagctaag ataccatcat ctggtgtgat aacttctcct gtacctgaaa
    21481 caagaagcat ttctttatca ttcattacga tcagcatcgc ttcaagtttt tgcatagatt
    21541 gctgtgtccg ccattcttga gctaattcga cagctgcgcg ttgtagattc ccgttatatt
    21601 cgtttagttt gccttcaaat ttttcttcta aagtaaacgc atctgcaaca cttccggcga
    21661 aacctacaac aacttctcca ttgtagatac gtcgtacttt ctttgcagtg cctttcatga
    21721 caacagattc acccattgtt acttggccat ctccagccat agcaaatttc ccgtcttttt
    21781 cgacggcaca gatagtggtt gaatgaaatg gtgattcgac catgtttgtt tcctcctaaa
    21841 tttgttcttt tccttatttt tacgctcttg gatggaaaga acgatagttt ttttgtaaac
    21901 tttcttttgt tacatgggca tagatctggg tagtcgataa attggcatgt cctagcaatt
    21961 cttggactgt ccgcaaatct gccccattat ttaataaatg agtagcaaat gtatggcgca
    22021 gcatgtgagg atggatatca ctatccaaac tgcttttttt gatcatttga ttcaagacat
    22081 attcaattcc agtaggcgtg attggttttc catgatgatt cacaaaaacg atatcatgct
    22141 cttcgtgata ttttgtcatc aatacctggc ggccttccct gaaatattct tgcagagcat
    22201 cttgagcaaa agaacctaga ggagcatatc tctctttgtt ccctttacca tggatcaaca
    22261 tgacctctga atcaaaatca atgtctgata ccgacaaact gctacactca cttaaacgga
    22321 tgcctgaccc gtaaaggact tccagtaaag cgcggttcct caaatccaaa ggactatctc
    22381 ctgctacgct gtcaaaaagt gcttgcattt cattttcata aaaaaaacgt ggcagtctca
    22441 aatttttctt tttgagatga acgtaagaaa atggattttc ttttatcact tcttctttga
    22501 gaagatactg ataaaacgaa cgtaggctgg ctatcttgcg actgatagaa tttctgctat
    22561 agctaggacg atgatcagct aatgcactca aatagacacg aaccgtaaaa tattccgcct
    22621 tcagcagatc agcgtctcca gattcctcca aaaaatgttt gaaatggaga atgtcttctt
    22681 catatgcttc tttcgttttt tctgaatatc ctctttcgac aatcaaataa cgcaaaaatt
    22741 tttgttccca cgtggcttct gtcattttct caccttcctt ttgtcccaga gaataattta
    22801 gcacagcgtt tataaaaaga caaataaatt gtcagaattt aaccaatatt tctaagtttg
    22861 ttcacaattg gcgcaattcc tcacattttg tttgagaaat tcttaagaaa aacgcaagaa
    22921 aaagagcgag actatccgtc tcaccctctt catggagttt ccttactcat ttgggattgc
    22981 agttttttct aattctgttt ttattgcctc attagctttt aaagctcgtt gtgcaattgc
    23041 ttcatatctt gcttttttgt ctcgaatgcg ctctggcagt tcaggaaaca gaccatagtt
    23101 ggcattcatc ggttggaaat gttttccttc cgcatgagta atgtagtaag ccatacttcc
    23161 aatagctgtt tcttgtggaa actcgactgg ttccatttct tttgctaagc gtgcggcgtt
    23221 gattccagca agtagtccgc tcgctgcact ttcaacatag ccttctacac cagtcatctg
    23281 acctgcaaag aacagatctt cgcgtttcgt tgattggtaa gtcggtttca gcaattctgg
    23341 cgaattcata aagctgtttc ggtgcatcac accatagcga acgaattcag cattttctaa
    23401 cccaggaatc atccggaaaa cacgtttttg ctcgccccat ttcaagtgag tctgaaagcc
    23461 cacgatgttg tataaagaag ctgcagcatt gtcttgacgc agctggataa cagcataagg
    23521 acgtttgcca gttttaggat cttccaatcc aactggtttc ataggtccga aaagcatcgt
    23581 tttgaatcct cgctgtgcca ttacttcaat tggcatacat ccttcaaaat atttttcttt
    23641 ctcaaaagat ttaagttctg ccacttcggc attgattagt gcctcatgga aagctttgaa
    23701 ttcctcttct gtcatcggac agttcaagta agctgcttct cctttgtcat agcgggactt
    23761 caaatatact ttatccatat cgatcgtagc tttgtctacg attggtgcag ctgcatcata
    23821 gaaataaaaa ccatctgaac cattgaatgc tttgatgctt tctgctaatg gatgagaagt
    23881 caaaggtcct gttgctacta cggtgattcc ttccggtatt tcagtaattt cttcgtttat
    23941 cactgttact aatggatgct ctttgacttt tcgggtaatt gtttcagaaa aagaatcgcg
    24001 gtcaacagct aaggcaccac cagctggcac tgctgtttca tctgccgaag tgattactac
    24061 cgaattcaat cggcgcattt cttctttcaa taaccctact gcattagcca aactgtttcc
    24121 tcttaatgaa ttggaacata ctagttcagc aaaattactt gtgtgatgcg cctcagtcga
    24181 tttgactgga cgcatttcat ataaacggac agggactcct gcttgtgcga tctgccaagc
    24241 agcttcactt ccggcaagcc ctgctccaac aacatttacc attttcaatg aaagaacctc
    24301 ttttctatta tttttggatg tcttcttcgt aatctccatt tacacagact acttgtttac
    24361 cgcctttgac tttcttctcg accagatatc cgtcacattt tggacaattt ctgcccactg
    24421 gtttatccca agaagtgaat tcacattctg ggaaacggga acaaccatag aatagtctgt
    24481 ttttcttaga ttttcgttca atgacttgtc cttcatgaca tactgggcaa gtcacaccga
    24541 tttcttttac gattggtttc gtattccgac aatcaggaaa attactgcaa gcataaaact
    24601 tcccatattt ccctaatttt atcaccatcg gatgtccaca gacatcacaa tcgaatcctg
    24661 ctggttcatc tttgatttga attttttcga ttttttcttc cgcattcgtc agttcttttt
    24721 caaacggttt atagaaacga tcaaccactt tgacccacgc ttccgtgcct tcttcaattt
    24781 tatccaaatc tccttccatt tctgctgtga aatggatatc gacgatttga gggaagaatt
    24841 cacagattaa tgaattgaca atttctccta gttcagtcgg ctcaaatctt ttttgtgtca
    24901 attttacata gtagcgtctt tggatcgtat caagtgttgg tgcataagtg gaaggtcgcc
    24961 ctaccccgtt ttcttctagt gcacgaatca acgtagcctc actaaaacgt gctggtggtt
    25021 gagtaaaatg ttgttttggt tcaatatcaa cagattggac tacatcgcct tcttcaagtt
    25081 ctggaagaat attttctttc tcttctttgc cgtcatctct accttccaca tacacttgca
    25141 tgaatccttt gaatttgatt tttgaaccgt tcgcaatgaa gatgacacca ttttgttcta
    25201 atgtcacttt cattgtatct aaaatagctg gtgtcatctg actagccaca aaacgtgacc
    25261 agatcaatgt ataaagtttc aattgatctt tatctaaata ttgtttgagt tcatctggtg
    25321 tacgaagcac actcgttgga cgcacagctt catgggcatc ttgtgcgcct tgagcatttt
    25381 tgatctttcg gtgatcatgt ctagaaaatt cttttccata cgtatcttca ataaatgctg
    25441 cggcctctgc ttttgctgta tcggcaatcc gggtagaatc agtacgcatg tatgtaatca
    25501 agccgactgt tccttgttta cccaaagtga ttccttcata taattgctgt gctaccatca
    25561 tggtttttct tgttcgaaag tttaacttac gggcagcttc ttgttgcagg ctacttgtag
    25621 tgaaaggatt cgcagggttt cttttgcgct ctttcttttc tactttttta acttcgaagt
    25681 ctgttccatc aattctagca gtgacttctt ttacactttg tgcatctggt aatttctttt
    25741 tcttaccgtc gattccccag aaatttgctt tgaatttctt gcgagctttt ttaaaattac
    25801 cgtcgatgct ccaatattct tctggaacaa attctcggat ctctttttca cggtcaataa
    25861 taatttttaa agcgacagat tggacacgtc ctgcacttaa ccctttcttg accttacgcc
    25921 agagaatagg actgatcgaa taaccaacga ttctgtctaa ggtacgacga gcttgctgtg
    25981 catctactaa atctacatcg atcgttcttg gttccttaaa agctgctttg actgcgtctt
    26041 ttgtgatttc attgaagacc acccgatttt tttctttcag atccaaccca agtaggtacg
    26101 ctaaatgcca agcaatcgct tctccttctc tatccgggtc acttgccaag taaacttttt
    26161 gagctttttt tgctgcggct ttcaggcttt tgatgacatc gccttttccg cgtatagaaa
    26221 tataatgggg ttcataattg ttttcaaaat cgatacccat tttacttttc ggtaaatctc
    26281 ttatatggcc aacactggcc attactttat agtttcgtcc aagatatttc tcaattgtct
    26341 tggcttttgc aggtgattct acaatcacta aatatttata cgccattact gaatggactc
    26401 ctttccattg tcgtgttcct actattaaaa gaggattcat acaaatcgta gctaatcata
    26461 tccattcaga aaacgtttgt caagaaaagc aaatgaaaag tatggtgtta ttctttaaat
    26521 tctgccaaga tatcttcacc cgaaaccgtg caaactgcac cgtcttggat caaatgatga
    26581 catccggtag aatgttctgt taaaatattt cctggaacag aaaaaactgt tcttccaaga
    26641 tccaatgctt gctgtgctgt tatcagagag ccgctttttt tcttggcttc aaagacacat
    26701 gttcctttag acaaagctgc gataatccga tttctcattg gaaaacaatg ctttttcggg
    26761 ccggattgag gaggatattc actcaaaaga agatgatcat ttgcaatcac ctcttgcaag
    26821 tcacgatttt cagcaggata gacttgctct atccctgttc caatgactgc gattgtccgt
    26881 ccaccatgag agattgctgc ttggtgaccg tatgtatcta tccctttcgc gcagccactg
    26941 acgatcacat atccagcttc tatcactggt tttagtaaat aataaactgt ttttttggct
    27001 aaagcagaag tgtgtcttcc accaacaacg cttaacatgt tacaagaaag taaagataga
    27061 tcaccattat aaaacagtac aattggcgga taggtcaagt gaagcagctg agggggatat
    27121 ttgggatcaa agcaagtgat aaacgactgt ttcgattgga ttttgcccca gttagcgtga
    27181 atttcctgcc aacttttgcg aaacagctcc cggtgtttgg ttattttacc tatcttaatg
    27241 atgtcttcaa atccgatttc tttataagga tactgcatca tccagttagc aagtgcccat
    27301 tttccttgac tactgattcc ttttgtataa gcaagtttca ataaattttc ttctatttga
    27361 tacatcataa aaccgccttt cgtctctctt tctaataaga tacgtaaaag cggctgtttt
    27421 ttattgtaaa tattttttta caggtgcaaa acttttacga tggattggcg taattccttg
    27481 tcgttcaagt ccttctaagt gttcctttgt tccataacct gcattatgcg agaaaccata
    27541 tccaggataa agcacgtcat attctttcat caattggtct cgatatactt tagcaatgat
    27601 acttgcagca gcaatcgaca cagatcgtgc atcccctttg attatttttt cctgctgaat
    27661 aggcaaatcc aaggtcatcg catcgatcaa caaatagctt ggtacaatgg acaatttttc
    27721 tactgcttgg atcatcgcta atttacttgc ttcataaata ttcacatcat cgattgtctg
    27781 ctgatcaacg atcccgatcc cgattgccag ggctttttct tgaacttgtt gataaagaag
    27841 ttctcgcttt ttctctgata attgtttgga gtcattcagc cctaatatga tctcattttt
    27901 cggcaagata actgcagctg ccacgacagg tccagctaat ggtcctcttc ctacttcatc
    27961 aatgccggca atccattggt gaccttgtgc gtacccttgc ttttcaaagc aaaacatttc
    28021 ttcatatttt tccttaatta atgcttcctt tttcaatcgt cgttcaaaag aagccagtgc
    28081 ttgctgcacg cctttgcgtg gatcatttct taatgattct agtgtaggat catctattct
    28141 ctgtatgctt ttcaatcgtt ctttgatact ttggatggat tcagtcatca cttttttgct
    28201 ccatttcttc gcaacgatcc agtgtgtatc gtccaagttt tccttgacga atatcatgca
    28261 cgactaattc gctgccacga tcatagtctt ctttataccc acgttttgct gtaatatcca
    28321 tcagtaattc cggtgccaat ttttcttctt gctcgattga taaaccataa cgatcgctga
    28381 ttcttcctga ataatatcgg gcaaaaaagt ctaatccgta aatcgctaaa tcatctaaat
    28441 ggacaagctg gtctttgatc gcgcctgtca gcgctagttt tttgccgatt tcttcatctt
    28501 caaatttggg ccataaaatc cctggtgtgt ctaacaattc caactcatta cctaaacgaa
    28561 gccactgctg accttttgtt acgccaggct tatttcctgt ctgtgcaatt ttcttgccta
    28621 ctagtcgatt catcaacgta gatttcccaa catttggaat acctaagacc attgcccgaa
    28681 tagcgcgagg cttgatcccc cgagcagcat ctcttgcccg tttttctttc aatgcttctt
    28741 tagccgcagg tactagacct ttaattcctt tactttcttg tgccgtgata gctaatgcaa
    28801 gatggccttg tttacgaaaa tattcttgcc acagctttgt ctgttgtgga tctgccagat
    28861 ccgctttatt caataaaaca aggcgaggtt tttgctgaat gatctgatct agcattggat
    28921 tacgagaaga taaaggtaat cgtgcgtcta ccaattcaaa tacgatgtcc acatatttca
    28981 ttttttcaga aacttctcgt cgggcttttg ccatatgtcc cggaaaccat tggatggtca
    29041 ttgttctctc cattcctttc ataccggaat attccccggc tttacttgtt tttttctttt
    29101 tcaatataaa ttcacttttt tattataaag aaattccatc tattagttaa gtgaaagtga
    29161 accagtcctc attctatcac atcttcactt ttttgatgat gcaaattttt attttggttt
    29221 ctatatatga atctaaattt ttgacagcgt ttcctaaata agagatgata gacgaacaaa
    29281 acagaaagga gacgaaacaa atgaatagta tggtttttgt taattttcct gttgctgatg
    29341 tcaatcgttc cgttgctttt tatgaaaagc tgggttttaa aaaaaatgaa gaattttcta
    29401 ctg
//
LOCUS       DASJON010000010        28076 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJON010000010 DASJON010000000
VERSION     DASJON010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.010"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            854..2023
                     /locus_tag="U3989_000338"
     CDS             854..2023
                     /locus_tag="U3989_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1713811.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            2496..3050
                     /locus_tag="U3989_000339"
     CDS             2496..3050
                     /locus_tag="U3989_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1713812.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(3223..3696)
                     /locus_tag="U3989_000340"
     CDS             complement(3223..3696)
                     /locus_tag="U3989_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1713813.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3708..4244
                     /locus_tag="U3989_000341"
     CDS             3708..4244
                     /locus_tag="U3989_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1713814.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            4284..4655
                     /locus_tag="U3989_000342"
     CDS             4284..4655
                     /locus_tag="U3989_000342"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1713815.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            4939..6210
                     /locus_tag="U3989_000343"
     CDS             4939..6210
                     /locus_tag="U3989_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1713816.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            6455..7480
                     /locus_tag="U3989_000344"
     CDS             6455..7480
                     /locus_tag="U3989_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1713817.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7774..8883
                     /locus_tag="U3989_000345"
     CDS             7774..8883
                     /locus_tag="U3989_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1713818.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            8966..9862
                     /locus_tag="U3989_000346"
                     /pseudo
     CDS             8966..9862
                     /locus_tag="U3989_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            9878..10702
                     /locus_tag="U3989_000347"
     CDS             9878..10702
                     /locus_tag="U3989_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1713819.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10734..12044
                     /locus_tag="U3989_000348"
     CDS             10734..12044
                     /locus_tag="U3989_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN1713820.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12192..13451
                     /locus_tag="U3989_000349"
     CDS             12192..13451
                     /locus_tag="U3989_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1713821.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            13461..14333
                     /locus_tag="U3989_000350"
     CDS             13461..14333
                     /locus_tag="U3989_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1713822.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            14345..15175
                     /locus_tag="U3989_000351"
     CDS             14345..15175
                     /locus_tag="U3989_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1713823.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            15215..17398
                     /locus_tag="U3989_000352"
     CDS             15215..17398
                     /locus_tag="U3989_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1713824.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            17459..18679
                     /locus_tag="U3989_000353"
     CDS             17459..18679
                     /locus_tag="U3989_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN1713825.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18676..20136
                     /gene="gtfA"
                     /locus_tag="U3989_000354"
     CDS             18676..20136
                     /gene="gtfA"
                     /locus_tag="U3989_000354"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN1713826.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            20213..21319
                     /locus_tag="U3989_000355"
                     /pseudo
     CDS             20213..21319
                     /locus_tag="U3989_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21641..21850
                     /locus_tag="U3989_000356"
     CDS             21641..21850
                     /locus_tag="U3989_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1713827.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21843..21992
                     /locus_tag="U3989_000357"
     CDS             21843..21992
                     /locus_tag="U3989_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN1713828.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22146..22811
                     /locus_tag="U3989_000358"
     CDS             22146..22811
                     /locus_tag="U3989_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1713829.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23095..24180)
                     /gene="xerS"
                     /locus_tag="U3989_000359"
     CDS             complement(23095..24180)
                     /gene="xerS"
                     /locus_tag="U3989_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN1713830.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            24514..24747
                     /locus_tag="U3989_000360"
     CDS             24514..24747
                     /locus_tag="U3989_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN1713831.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24744..25151
                     /locus_tag="U3989_000361"
     CDS             24744..25151
                     /locus_tag="U3989_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1713832.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26288..26806)
                     /locus_tag="U3989_000362"
     CDS             complement(26288..26806)
                     /locus_tag="U3989_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN1713833.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27924..>28076
                     /locus_tag="U3989_000363"
     CDS             27924..>28076
                     /locus_tag="U3989_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1713834.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         9628 a         4253 c         5144 g         9051 t
ORIGIN      
        1 aattttctac gttttccttt tcatatatat taaatattac ggaaaattat ataaactaat
       61 ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt
      121 taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc tttcgtactt
      181 aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata
      241 tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct
      301 ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta
      361 tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga
      421 ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt gataataaag
      481 tactacctat taaaggtagt actttattta atttagtata aattaacata ctactatatt
      541 ttaggtttca tattatataa attaataata tactaccatt tgttggtagt ataatttaaa
      601 ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa gtttaataag
      661 agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct ttagtgatat
      721 aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa tagatattta
      781 gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg aaattttata
      841 ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa tcaataaagg
      901 tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat tattgcaggc
      961 tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca attcaggtaa
     1021 ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat taaatttggt
     1081 aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac
     1141 caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga
     1201 aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa ccatttcaaa
     1261 tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct tagaatccag
     1321 atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa ctgtttcaaa
     1381 agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg ttcttgggtt
     1441 tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag acctaaaaga
     1501 ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca ttaacgatag
     1561 tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa
     1621 tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt
     1681 ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga tagataaatg
     1741 gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat taacctttta
     1801 taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg aaggttttaa
     1861 caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag aatctctgga
     1921 gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc gagttcataa
     1981 aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc aacagaatgg
     2041 gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct tgcgtaaagt
     2101 tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg ttattcatat
     2161 gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa acttaaattt
     2221 catataattt aattatgtaa attagctatt ctaagaactt ctacggataa ctgcccgtct
     2281 aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt tgtacaaaaa
     2341 agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc ccttgatttt
     2401 attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat atacataatc
     2461 attaactcag aatattttta agaagggaaa caatagtgag ctataatgta caaccgttaa
     2521 ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt aatccggaaa
     2581 gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca gatattatag
     2641 gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa cataaaacag
     2701 gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta tatactgaag
     2761 gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat ataacaaggg
     2821 atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat gatataggaa
     2881 cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga gacattgcta
     2941 ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac attggtataa
     3001 ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa tttaaaaatt
     3061 tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat tttatttcat
     3121 aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt gttatataaa
     3181 ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa agagaatcaa
     3241 tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact caacgtttag
     3301 cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt tgaccttctg
     3361 caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa ttgccgtttc
     3421 ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt taatatctgt
     3481 atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga gcagactaca
     3541 atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt taatgatgat
     3601 tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg atgaaaacaa
     3661 aagttgttca ccatctttta taggtttgat tctcattttt agatattatg cccgcacgta
     3721 ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt tatcataata
     3781 tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc atgtatcacc
     3841 tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt gaagtacaag
     3901 aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta aaagttcctt
     3961 ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt tgccattatg
     4021 gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat gcaacacgat
     4081 tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg tatgtcacag
     4141 accagatccc tacagagcat gccattttag gaattgatgc cattcaacta cgcgtacaat
     4201 attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa gaatacgtca
     4261 gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact cagttctatc
     4321 aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg attggtagcg
     4381 tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc gacaaacaat
     4441 tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt gtatcacttt
     4501 attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga aacctagatg
     4561 caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt ttaccgcagt
     4621 acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc catgacgaag
     4681 agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc cattctaaag
     4741 aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta ataaaactac
     4801 ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt aaactaaaca
     4861 tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat aaaggggagt
     4921 accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa aaatgtcagt
     4981 ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc aggaaaatca
     5041 tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga cacctatgat
     5101 agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga gattgataat
     5161 ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg ctcaacgtta
     5221 gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt tgcaacacct
     5281 agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg ttctgtctgt
     5341 cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc ggctattgat
     5401 aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg aattgaggaa
     5461 caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc agaagatgtt
     5521 aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt acgtgtaaaa
     5581 gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga tacgcatctt
     5641 tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt aaatacaaat
     5701 gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca tgagaaaata
     5761 gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga acgacatcaa
     5821 gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg gatatataac
     5881 aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat tgttgttact
     5941 ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa acgagtgatg
     6001 gaacaattat cgattattgg atttgatgat attacttttg cagaattaat ggatcctgcg
     6061 ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca ccaactgatt
     6121 ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg agaattgatt
     6181 tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt atagttaaca
     6241 ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc gtcgcaaatt
     6301 cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt ttttaattat
     6361 gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga ttatatgtta
     6421 gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg atgttgcaaa
     6481 acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc cgacactttc
     6541 agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc attatgtcaa
     6601 aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag tttctgaagt
     6661 agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag tcgaagcaga
     6721 acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt tggagcaaga
     6781 cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt ctgtgattga
     6841 agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta ttgaagctga
     6901 agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag atcgattgta
     6961 taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt tagatagcca
     7021 aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga cgtggatgaa
     7081 acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca cacttgacgg
     7141 aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc cgactgctat
     7201 catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct
     7261 acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg ctgaattttt
     7321 aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac tagcagttcg
     7381 catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga ttgtttctaa
     7441 taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa
     7501 gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa aacattaagt
     7561 aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg aattaagatg
     7621 aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac attactcgca
     7681 gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag ctatgcgagt
     7741 tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga aaaacaaaat
     7801 cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt taaattagaa
     7861 gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag cgaatcctat
     7921 aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga aaatgatatt
     7981 gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga cgaactatac
     8041 gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt atttgataat
     8101 gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca agctgcaatt
     8161 gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg aacagagact
     8221 ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga caatacaaaa
     8281 tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga tattgcactt
     8341 gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt aagtcgtttt
     8401 caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg atttttgaaa
     8461 aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa agttaatgca
     8521 acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc agaagaagtg
     8581 aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc ggaaacgggg
     8641 gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc agatacaatt
     8701 gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata caataaagat
     8761 ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc tggtgaagta
     8821 actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt gagcggaaat
     8881 taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg tatattaatg
     8941 gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat atcgtaaaga
     9001 tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa ttttattaaa
     9061 tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag ggttcggtga
     9121 tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg aagtttttca
     9181 gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg ttgtattatc
     9241 cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt atcggacaat
     9301 ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc gctggatgtt
     9361 caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg atggaccagc
     9421 atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag tttggagtgc
     9481 tattggttat aacatggtgt tattactagc cggcttataa gaaattccta aagattatta
     9541 tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca ttacgttacc
     9601 attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg ctttccaagt
     9661 atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta aatcacaatc
     9721 attagcctat ttattctata aacattcatt tattttaaat gacaagggat atggttcggc
     9781 aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa tgaagttgca
     9841 gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat ttttttcaaa
     9901 tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac cttttatttg
     9961 gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac ctacaatttt
    10021 ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac cattcatggc
    10081 tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg ttttattttc
    10141 agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata tccttttcag
    10201 cttagtactt attcaaatga tgattcccgg ttccattttt attactccac agtatttatt
    10261 agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag gggtagttag
    10321 tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg aattggagga
    10381 agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta tgttgccgct
    10441 tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat ttaaagattt
    10501 aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc cgggattagc
    10561 aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt cgttattagc
    10621 aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg aaggaattgc
    10681 tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag tacatgacaa
    10741 aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga
    10801 tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat gaaaaacgac
    10861 taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg
    10921 atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt gttatcaatg
    10981 ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt cctaaaaaat
    11041 atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt agaaccctac
    11101 ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt ccaaatgctt
    11161 ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta aaagcgacga
    11221 gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc
    11281 atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt gcaggcatta
    11341 atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat ccaacaattc
    11401 gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt gaattgttga
    11461 aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac catccatact
    11521 ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg gatgaatact
    11581 tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac attgtaaata
    11641 atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg gattctatta
    11701 cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta atcacaaact
    11761 taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa
    11821 cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat atcaatgttc
    11881 aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat caagcagcgt
    11941 atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat ttagtggatg
    12001 atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa gctgaaagag
    12061 taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat attgacaaaa
    12121 cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg
    12181 aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa
    12241 cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag
    12301 agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct aaagattttg
    12361 aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa
    12421 ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat cctcaaagta
    12481 tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa gaaccgtatt
    12541 tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt gtatatagca
    12601 taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt gaggaaatgg
    12661 ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag attaaagatc
    12721 aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat ggttatcacc
    12781 aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt tggcgtttct
    12841 cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt aatcgattag
    12901 atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt tataacgata
    12961 cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc
    13021 cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt ccagcagatg
    13081 aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt tctgcaactt
    13141 ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct gaagcgatgc
    13201 agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc gagaatactc
    13261 aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat ttagtttggt
    13321 tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat tatttacaca
    13381 atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca atgaaagctg
    13441 acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac tgggcaatca
    13501 tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca atggttcaag
    13561 gtgcttttta cagtttaact aattggactg gtctgactta taattttgat tttgtgggtt
    13621 tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt ggcttcactt
    13681 taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg attgcgcggg
    13741 cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt tttccagcgg
    13801 tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat ggtttaccaa
    13861 aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga acaacatggg
    13921 gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg ccagtcatca
    13981 ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct agaatggatg
    14041 gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta ccaagtattt
    14101 caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa atttttgctt
    14161 taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt tataattatg
    14221 cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta tttatcttaa
    14281 ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc taggaggttt
    14341 cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat tattaggggg
    14401 cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa caacacccga
    14461 tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt tccgaagatt
    14521 gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa tcattgcagt
    14581 agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa aaatgactcg
    14641 tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg ttcctttcca
    14701 agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg
    14761 attaatctta ctctatctca cttatgccgt accacagaca ctatttttat atgttggttt
    14821 tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg gggctagtca
    14881 ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg cgacgactat
    14941 tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa ttttaaatcg
    15001 tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac aatattttaa
    15061 cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta cactagttta
    15121 tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga aataaccaat
    15181 aaattattct cattataaag gagttcataa acttatgatt atatttgaca atcacacaca
    15241 aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag aaaataatta
    15301 tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt atcgctaccc
    15361 ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt tattatcttt
    15421 agacacatta ccattagaat ttccaggata tggacatggt gattttagag aaccggcgca
    15481 taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata gctacgaaat
    15541 tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag atgatgatga
    15601 agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt tacagttgtt
    15661 ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag taaataatag
    15721 tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc caaatcgtcc
    15781 actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa
    15841 aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc atcaacaaaa
    15901 tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg catatggatt
    15961 taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat ttgaccaaac
    16021 acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa ctggcgactc
    16081 atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata cgttatcaca
    16141 gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt taaaagaacg
    16201 tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag aaaaaatcat
    16261 gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag atgatggttg
    16321 gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa cagaaggcaa
    16381 acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga tgaaatttgg
    16441 gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag cacatccaga
    16501 ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga
    16561 tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga tttttgatcg
    16621 tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag tctattctgt
    16681 cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag ggttatatga
    16741 atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt gttcgggtgg
    16801 aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga cgagcgacaa
    16861 taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt atccaatttc
    16921 aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag aaacgagttt
    16981 aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag atctaacgca
    17041 attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata aaaagcatcg
    17101 ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag gcaatgatac
    17161 tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt tccgagtatt
    17221 agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag aagaagcaac
    17281 ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg gtttttatat
    17341 tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac agatatagta
    17401 gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg aagaaaatat
    17461 gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa atgaagaagt
    17521 acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag gcggatttag
    17581 aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg aatctgatgg
    17641 agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca ttgaagtgtg
    17701 gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg tagcagaagc
    17761 cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt attcgatggt
    17821 ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt ttaaagatag
    17881 ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag ttacgcaaga
    17941 taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa ttaataaata
    18001 tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc tttttatgtt
    18061 actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca acatagaaat
    18121 ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac gggcattaga
    18181 atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc gggataattc
    18241 tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt ctgataatgt
    18301 atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt taaccaatca
    18361 acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata gtgtatacaa
    18421 agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac atattttgct
    18481 ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta cagcaacaga
    18541 acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt atgaagatgt
    18601 tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg tattagctaa
    18661 ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta tgcagatagt
    18721 ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca aggtgttatt
    18781 ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt tgcgccgagt
    18841 gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc attaggtgaa
    18901 gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc aaaatttttc
    18961 caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat ccgtattcat
    19021 gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta caagcgtaaa
    19081 gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga ggtttggaat
    19141 acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa agaatttatt
    19201 cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt agatgcgttt
    19261 gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga tatttgggaa
    19321 cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt accagaaatc
    19381 catgagcatt attccattca aatgaaaatt gccaatcatg attattatat ctatgacttc
    19441 gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg tttagccaat
    19501 tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga tggaattggt
    19561 gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc tgaggaattg
    19621 tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa taatttagat
    19681 atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg tagctatctg
    19741 ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta cgttgggtta
    19801 ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg taatattaat
    19861 cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt gattcaacaa
    19921 ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt
    19981 gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa aaacggtagt
    20041 gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga gtctaaagat
    20101 ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc
    20161 tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat acatggataa
    20221 gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag gaaaactttc
    20281 tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac tgtcttttga
    20341 agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt
    20401 gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat cgattagtca
    20461 tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag aaatttttca
    20521 acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc atctgtatct
    20581 gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga ctttccgtca
    20641 aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata ctacgatgta
    20701 tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac taccagtatt
    20761 ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact ttgaaaagat
    20821 ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa acaccaaagt
    20881 tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag acgagctggt
    20941 tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg ttcaagataa
    21001 gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc
    21061 tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca aacaacacat
    21121 gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac
    21181 gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg aaaaatcagt
    21241 gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat gttgtctaga
    21301 aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt tttcagttga
    21361 ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag ttttttttga
    21421 cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca acactagtga
    21481 tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc aagtatacaa
    21541 agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact atttttagta
    21601 gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta taacaaaaaa
    21661 tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac gacaggcaaa
    21721 agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag gactagtacc
    21781 tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg attttcagaa
    21841 tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct agtttgggat
    21901 tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa cagttaaaga
    21961 agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga aacttctcaa
    22021 agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta tcaagggaaa
    22081 gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt tcttatttct
    22141 ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa aataatttat
    22201 tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag atgtcattag
    22261 atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt ggaaatgtga
    22321 agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa gttatttcaa
    22381 ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg ttacttttta
    22441 tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt gattttgaaa
    22501 ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc tatgagtttg
    22561 tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat acaattacct
    22621 ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt gtactttctt
    22681 ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga catgcaacga
    22741 tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct tctttgctta
    22801 tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct gatgtacaaa
    22861 cgactttagg aacttatggt catttgtatc taaattcatt gagagagata acaaatgatc
    22921 ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt tacgataata
    22981 aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag aaaaactata
    23041 atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt atactcataa
    23101 agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag ttgttgtatt
    23161 ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct gttggtagag
    23221 tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa agccttttga
    23281 atattttgct accatccgtt cgattgcaat accggatata cgcttggctt ctccttggta
    23341 aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt tccgaatagc
    23401 tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat ctcttttatt
    23461 tccttttcta gttactacca cattattctt atctaaagat aggtcatcta aattaatatt
    23521 tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg ccaggtctct
    23581 ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt tatattcatt
    23641 ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt tctcaatggc
    23701 attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat ccataggatt
    23761 attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat atttaaatag
    23821 aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta agttattatt
    23881 atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc gacgtttcaa
    23941 atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg tgatagaagt
    24001 atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat tttttagata
    24061 ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa aatcttttat
    24121 ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg tatttttcat
    24181 gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga ctataccaat
    24241 tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc aaaaaaagca
    24301 tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata gggaaggaaa
    24361 tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga aagaacaatc
    24421 tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca agcgtatata
    24481 ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag aaaatttagg
    24541 gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga aaatgggtta
    24601 aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat tacaaaaaaa
    24661 caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga atatgatact
    24721 acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa gatattataa
    24781 aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt gtaaaggatg
    24841 cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt gaagaggttt
    24901 atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag aaacatcctt
    24961 ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg aaaatcaatg
    25021 gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat attgctacgt
    25081 atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag acacatacgc
    25141 agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct atagcgaaat
    25201 aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa acgtaaattt
    25261 tctatatagt tggattaaga taacgactat ttattttatc atatttttac tataccctat
    25321 acttttactt ggtatattta tgaataatcg caaatttacc cctatactac tagtttgtaa
    25381 gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc tacaacttat
    25441 aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag taaagtttgt
    25501 tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc tgaaaactta
    25561 tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac taagcaataa
    25621 aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat tctgataaat
    25681 gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga tctcttcagt
    25741 atcagtaata ggataggcga taggccacat accctttgat ggttagaata aactgctcaa
    25801 aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa ttataaacca
    25861 taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca gaagatataa
    25921 ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta aagggaattg
    25981 tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc ccctgctagt
    26041 ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga agtgagtcat
    26101 ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc tacggcattc
    26161 taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga acttatgtag
    26221 tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct tagcagtaaa
    26281 cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa gtcccaaatg
    26341 gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt ttggttgcct
    26401 cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt gaatcaatca
    26461 tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc aatggtagat
    26521 gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat ttaaattgat
    26581 cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt agaattaaat
    26641 tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact tcctcaactc
    26701 ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta gtataagtcc
    26761 tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata ttacctccat
    26821 aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt ttaatccgtt
    26881 ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga tttataaaat
    26941 atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa aatacgaata
    27001 tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta aatatcaaaa
    27061 tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta agaactgtta
    27121 agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga aagtggtaaa
    27181 actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg cgttgggtaa
    27241 aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc agatgaaaca
    27301 gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc gattgccata
    27361 ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc atccttttta
    27421 ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga tgttttcacc
    27481 agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga aaattcaccg
    27541 cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt ccaaaattaa
    27601 gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt ctttttaaac
    27661 aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt ttatgacttt
    27721 cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg gacgtttacg
    27781 agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg aactaccccc
    27841 acttagcttg gataggctac aattaactag acagaaaaat taaggtgtgt agactagaag
    27901 aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt tcacaagaat
    27961 tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag
    28021 aatatgaact aacggcttca gcatttgaca aatgggtaaa gcaatctaaa acgagt
//
LOCUS       DASJON010000011        27494 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJON010000011 DASJON010000000
VERSION     DASJON010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.011"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..344)
                     /locus_tag="U3989_000364"
     CDS             complement(<1..344)
                     /locus_tag="U3989_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713835.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
     gene            complement(416..1696)
                     /locus_tag="U3989_000365"
     CDS             complement(416..1696)
                     /locus_tag="U3989_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN1713836.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(1787..2239)
                     /locus_tag="U3989_000366"
     CDS             complement(1787..2239)
                     /locus_tag="U3989_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1713837.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(2252..2662)
                     /locus_tag="U3989_000367"
     CDS             complement(2252..2662)
                     /locus_tag="U3989_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1713838.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2937..3149
                     /locus_tag="U3989_000368"
     CDS             2937..3149
                     /locus_tag="U3989_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1713839.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            3152..3364
                     /locus_tag="U3989_000369"
     CDS             3152..3364
                     /locus_tag="U3989_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713840.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            3504..3659
                     /locus_tag="U3989_000370"
     CDS             3504..3659
                     /locus_tag="U3989_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713841.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3656..3880)
                     /locus_tag="U3989_000371"
     CDS             complement(3656..3880)
                     /locus_tag="U3989_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1713842.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3958..4146
                     /locus_tag="U3989_000372"
     CDS             3958..4146
                     /locus_tag="U3989_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713843.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            4143..4316
                     /locus_tag="U3989_000373"
     CDS             4143..4316
                     /locus_tag="U3989_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713844.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            4472..4807
                     /locus_tag="U3989_000374"
     CDS             4472..4807
                     /locus_tag="U3989_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713845.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5040..5981
                     /locus_tag="U3989_000375"
     CDS             5040..5981
                     /locus_tag="U3989_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN1713846.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5983..6873
                     /locus_tag="U3989_000376"
     CDS             5983..6873
                     /locus_tag="U3989_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN1713847.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6916..7716
                     /locus_tag="U3989_000377"
     CDS             6916..7716
                     /locus_tag="U3989_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1713848.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7731..8582
                     /locus_tag="U3989_000378"
     CDS             7731..8582
                     /locus_tag="U3989_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1713849.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8783..9244
                     /locus_tag="U3989_000379"
     CDS             8783..9244
                     /locus_tag="U3989_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1713850.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            9270..9722
                     /locus_tag="U3989_000380"
     CDS             9270..9722
                     /locus_tag="U3989_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713851.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9828..10022
                     /locus_tag="U3989_000381"
     CDS             9828..10022
                     /locus_tag="U3989_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713852.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            10053..10346
                     /locus_tag="U3989_000382"
     CDS             10053..10346
                     /locus_tag="U3989_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713853.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            10376..10816
                     /locus_tag="U3989_000383"
     CDS             10376..10816
                     /locus_tag="U3989_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1713854.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            10891..11358
                     /locus_tag="U3989_000384"
     CDS             10891..11358
                     /locus_tag="U3989_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN1713855.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            12105..12335
                     /locus_tag="U3989_000385"
     CDS             12105..12335
                     /locus_tag="U3989_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713856.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            12494..13327
                     /locus_tag="U3989_000386"
     CDS             12494..13327
                     /locus_tag="U3989_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN1713857.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13320..14735
                     /locus_tag="U3989_000387"
     CDS             13320..14735
                     /locus_tag="U3989_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1713858.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14747..16276
                     /locus_tag="U3989_000388"
     CDS             14747..16276
                     /locus_tag="U3989_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1713859.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16194..17246
                     /locus_tag="U3989_000389"
     CDS             16194..17246
                     /locus_tag="U3989_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN1713860.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17248..17565
                     /locus_tag="U3989_000390"
     CDS             17248..17565
                     /locus_tag="U3989_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713861.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17692..18315
                     /locus_tag="U3989_000391"
     CDS             17692..18315
                     /locus_tag="U3989_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN1713862.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            18330..19220
                     /locus_tag="U3989_000392"
     CDS             18330..19220
                     /locus_tag="U3989_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN1713863.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19243..19458
                     /locus_tag="U3989_000393"
     CDS             19243..19458
                     /locus_tag="U3989_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713864.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19470..19811
                     /locus_tag="U3989_000394"
     CDS             19470..19811
                     /locus_tag="U3989_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713865.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19811..20182
                     /locus_tag="U3989_000395"
     CDS             19811..20182
                     /locus_tag="U3989_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713866.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20175..20570
                     /locus_tag="U3989_000396"
     CDS             20175..20570
                     /locus_tag="U3989_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN1713867.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20572..20949
                     /locus_tag="U3989_000397"
     CDS             20572..20949
                     /locus_tag="U3989_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713868.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20950..21558
                     /locus_tag="U3989_000398"
     CDS             20950..21558
                     /locus_tag="U3989_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1713869.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21558..21899
                     /locus_tag="U3989_000399"
     CDS             21558..21899
                     /locus_tag="U3989_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713870.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22141..24930
                     /locus_tag="U3989_000400"
     CDS             22141..24930
                     /locus_tag="U3989_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN1713871.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            24920..25660
                     /locus_tag="U3989_000401"
     CDS             24920..25660
                     /locus_tag="U3989_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1713872.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            25657..>27494
                     /locus_tag="U3989_000402"
     CDS             25657..>27494
                     /locus_tag="U3989_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1713873.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9811 a         3957 c         5445 g         8281 t
ORIGIN      
        1 tcttgtataa gattactatt aacgtttttt acaagtacat catcttttat atattttgaa
       61 attaatgata gataaacctg agttgttcga taggaacttt cttttacagt tggcttgtat
      121 ttttcatccc atttagtttt taattctcca aaagttagtg gtaattcttc atttcgtttt
      181 attttttctg cgattttttc attcaaaagt aagttagctt gattccaagc ttggcgtgaa
      241 ttgctagata aagtaacgga cacttttctc tttttttcag ttaagggatc cgtataacgt
      301 tcggtgtact taaatttttt gtcatttatt ttttctaacc acattatttc tattcctttc
      361 gaatatacgt tcctattaat tgttaaaaga aaaagcccga aggcttttta tatatttaat
      421 cttttgacaa ttctaaagat cgtctatatt gttcagcttt agctaatttt gtaaatgtta
      481 ctgttccatt ataattttct aaaacaactt tctctatttc gtctagtgaa actttgaaaa
      541 attcttttct ttgattaatt ttattaactt cttgttttct aaaatgatta tgcaatttag
      601 tttccaaaga cggagcgtcc tctgaaaaaa tcatcgcatg aacgtcaaat tcaaatggaa
      661 ccgatgcact actcaattct tttattctat ccataggttc taatcttcta gtcataccaa
      721 ttttatatat gtcttcacca aaagacccaa tattactaat tacatacaca taaccggctc
      781 tagtatttaa ttctctttgt aatacatttt cacgtataac ttctaattct tttaattttt
      841 cttccagttc ttgaatttta tttgcatata attctttttc cacatctgca ttagcttttt
      901 gcatatatgc gattaatttg gaaatttcag aattgaattg acgttgttct ttatcaatac
      961 gtttcttttc acgatcaatt tctctacgaa ctttttcctc ttctatcatc tgttctttta
     1021 ttgctttttg ttgttctttt tcttcggtaa ttttcactaa ataagagtaa tgaagttcta
     1081 gttcttgaag tttaaactct aaaagaccct tccttaattc agcattgtca ttcttgaata
     1141 aattattaat tttatcaaat atttttatga ttttttccct agtcgagtct atattagaaa
     1201 tagttagttt attgagtaat cctttcactt ctgaatcaaa tggaatgagc atttttttct
     1261 ttaaattatt tttctctcgt ttatttaaaa taagagggac agaaacagca tcgtcaataa
     1321 tatattcctt ttcttttaat tttaacaaag aaagcttatt ttttatttca gaagatgaag
     1381 tttcttctat atttgaaacg ataagtgaag aatagacatt ttttaatgtt tcatcatact
     1441 tttgatttat ttcatcattt aaatcttctt tctctttttt taataaaata atatcatttt
     1501 ttaacatagt aatttgttta tttaaatctg atagtgtttc ttccttactt aatataaatc
     1561 tttctctatc atgtaattta tttagttttt catcattttt ttgttctaat tcataagctt
     1621 ctttttttat tgtgttagct ttcgacagtt tagtgattcc tataattaat aaaataagtc
     1681 caatcaataa taacattcaa aactcctcat ttctttgata taattattgt agagtttact
     1741 cagaaatgag gaaagagtcc atgtgaaagc atgggctttt ttatttttaa tgttgtaata
     1801 ctaaatgttc aagattatga ggaagcccta aatattcaat aatttggaac tgactcatat
     1861 cagataaatc ttgttcatct attgttctag ttaacatttc aattgcaaac atattagctt
     1921 cagcttctat accataaatc atacaactaa ggttgtttgc tctaaaagct gctgtgcaaa
     1981 tacctttatg gagtctaacg tgggctattt catgccataa tacaaatctt ttcatagaag
     2041 gtgataggtt ttgattaatt aatatcacat aacatctttt attgtgtatc gtttgtccta
     2101 atgttttatt ccctaaatca gtttcaatta aatctatatc agaatatttg attattttat
     2161 atggattaat cgcatcagat ccaaactcta caaataattt ttctatttct tctaatatcc
     2221 agtatttgtt taaatgcata ccaaagccca cctattgacg atatttttta ggagtgaatt
     2281 ttttctttgc tttaactttg gttaaggcaa gtgcaagttg aatggcatcg tataattttt
     2341 ccttatcttc atcgtctaaa ggttcaccat aataatttgt ttcagcatct gaattcaagc
     2401 cttcaagtaa tttttcagct tgtaaagcaa tatcttgttt atctttttct gttaaatcat
     2461 aataatggcg tttatcagta cgtcctaaaa gataatcagt tgatacatta aagtaatctg
     2521 caactttttg tattttatct ccagatggag tacttttatt ccatctagac atgcttccat
     2581 ttccgaaatt taactttctt tcaagttctg ccaacgtcat tgaacgttct tttaataaga
     2641 tttttatacg gtctaatagc ataggatatg cattcctttc aaaataccga caaaaaaagt
     2701 atttttctgc aaattatcta tttacaaata gagaatttgc agttatacta gttttgtaag
     2761 caagatattt acggcataca aagactaaaa gcaataaact aatagtctcc ccagacatca
     2821 gtaagctcgc tttttgtgcg ctgtattttg tatgcttaca taatagcata tttgcagttt
     2881 ttgtcaaact tattagacaa aaatattaga aaatattcga taagggggta tgaagcatga
     2941 ctttaaaaca gcaagtatta attgctttag taaagaaagg ttggagtcaa agagagttag
     3001 ctcgacgaat gggtatatca attacttatt tacgagatat cttcatcgaa aaaagaaaac
     3061 ctaaggaacg tttaaaacaa atcgaagaac ttttggatat caagttagaa gtagattcta
     3121 atgatcagcc atcagaacag gaggcgtaaa aatggtacaa cgtttgagca aagcggagtt
     3181 taaagagaaa tataagtatt ctgaaagtac ttatcaaaga agaatgaagg aatttaaaaa
     3241 atcacgtttt agtgaggggt ataacgcagt tacttccaaa gaaataacaa ttgatgtcga
     3301 tttatatgaa cagtttagaa catggcgttc taaaaatcga tttagagtta gagctgtttg
     3361 ttaatagcta aaaaatctaa attcaccact tctgaaaaga ggagttgctg ctctcctttc
     3421 aagtttgcca aagtccacgt tttttcattt tggcaactcc tcttcttgga ggtggtgacg
     3481 gcgagagacg aaggagggat gacatgaagg aagaatcaaa aatagggatt ttgaatgaga
     3541 acaattacac aagattaatt attgaaacag aagaagggaa aaaagtagca gaagttacat
     3601 tgaccgatgc aactcctgct gacggttatc gtattcgatt aactcctaaa tatgattaac
     3661 cttttggtgg gtgtggatcg tttccgtgac tatcacggct ttgaattttt ccatctctgc
     3721 catgaataaa tagctcagat ccttgatttt tagaaatctc tctagcgatt tttactgctt
     3781 ctgattttgt attagtatgt gcagtcgctt tagaatttcc agcacctttg acattccaac
     3841 caccattgga attaggtaca acatgttgat tttttcccat agttttcacc tccatttcta
     3901 tagaaatatt ttatcaaata gaaatggaga aattcaataa acgaaaggag aagtgatatg
     3961 gcatatacct tgcaacaaga acatcaaatt ctcggtttga ttaaacaacg caggaaacaa
     4021 ttacaagatg accgtgcagc gcttagaaaa gccgatgagc tatcagatag gcaagctgaa
     4081 ctaattgctt ctgaacttga ggatttgaga atgctagaaa taaaaaatag ggagattaga
     4141 ttatgaagaa gacagacaca ctttttatag gattcatttt ggggttatta gtgattgtag
     4201 cgcaccaaag tattatcggg ggaagcttgt tcgcagcatt gatggtttta atcaatctgc
     4261 ttgattcaaa agaaaggagc aactatggca cgagaagaag cgctaaaaat cggtaaagtg
     4321 attgctgata atcggtgggg aaatagccgc cctattattt taagcaagca acatatcgaa
     4381 aagcaaaaag catggcaaca aataaaaaag tgactctgcc agccagcaaa gagtcacata
     4441 caaatttata aaactaggag aattatatca catgaataag aaaattgaaa atcttatcga
     4501 agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt atttgtacag cccaaaaaga
     4561 aggggaactt aaaagtctcg tttatggtga gacaacagaa attttacttt gtcttgctat
     4621 gcaagaagaa catttagatg aaaatctccc attgtcagca catattatgc gtagaattgc
     4681 tgtggatgct tacgaacaag cgaaaaatga agaagaaaat caaccttcaa atcatacttt
     4741 tgttattaat aacaaggaag atttagctga tgtgatgact cgtattctca agggggaatt
     4801 tcaatgatga atgaaaaaac aattgatcta ctcattattt tatttttcac gttagtgatt
     4861 gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa ttaccatgct tgtgctttgc
     4921 gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg acaactattt
     4981 aactgatcct gattatgcta aaccaccata tgaagcacca attgatgagg aggatgaaga
     5041 tgagtaaatc taccttagaa atgagccatc aagaatggct tgaagaccgt aagaaaggca
     5101 tcggaggttc ggatgttgga acaattttag gattgaataa atggaaatct ccttatcaac
     5161 tatggctaga aaaaacagga caagtagtac ttgaagaatc aggaagtgag cccgcttatt
     5221 ggggtaatgt tttagaagaa gtggttgcta aagagtttca ggaacgtaca ggcaaaaaag
     5281 tacgcagaag aaaccaagta tttgaacatc cattacatcc atttttaaga gcaaatattg
     5341 atcgggaagt agtgggagaa aatgccattc tggaatgcaa aacagccaat caatttctcg
     5401 gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt caacattaca
     5461 tgaacgttct aaacaaagac tattgttaca tcgctgtctt aatcggtggt caaaaattta
     5521 tctggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa caattagtag
     5581 agttttggga aacaaacgtt cttggaggta tcgagccaat tattgatggt agtcaagcga
     5641 cagctgattt cttgaaagag aagtatgcag atgttgaaga cgttcaaacg acgttaccaa
     5701 ttcatttcga tgaactagtt gaacagaaaa acgaactcaa acggactaag aaagaaattg
     5761 aatcagctat ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa cgtgaagcca
     5821 gtatcggtat cactcaaaaa aacatcatca gttggaaatt tgtccgtacg agacgtatga
     5881 actcgaagaa actagcagag aaatatccag atgtcgcaaa tgatgaagag atttataacg
     5941 ttactgaatc aagaaggcta accgaaaggg agatcaaata atatggcaac aaatgaatcg
     6001 ttaaaaaatc aattggcagc aaagccacag aaacaagttg caccaggtca attaggactt
     6061 aaagctttga tgaatacacc aacaatgaga aagaagttcg aagaggtgct tcatgacaat
     6121 gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta tcttgcagat
     6181 agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa tcttgggcta
     6241 gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa gcaaacagga
     6301 aaatgggaaa agaaagctca attcatgctt ggctataaag gatatatcca attagcccaa
     6361 cgatcaggta aatacaaagc attaaatgtg atcgaagttt acgaaggaga actaaaaagc
     6421 tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacgtc tgatgaagtc
     6481 attggatatg ttggctattt cgagttactg aatggattca aaaaaactgt ctattggacc
     6541 aaacaagaaa tcgaagctca tcggattgct aacaataaag atcgcgataa gacaaagtta
     6601 agtggtgtgt gggcatctga ttacaatgca atggcacgaa aaactgtttt gagaaatctt
     6661 ctttctaaat ggggaatctt gtccatcgaa atgcaagaag ccaccacatc ggatgagaga
     6721 gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa cagaagttga agaagatatt
     6781 cctgaaagaa aagaagcaga ggttatatct gaagaaaatg aagatgtaca aactggatta
     6841 tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gctttctccc ttgcttttct
     6901 agaggagaaa tacgaatgaa cacaggatat gtcaaattac atcgaaaagt gatgaattca
     6961 ttcgtttgga ccaatcctta tatgtataaa ttatggaact tgtgtttgat gaaagcgagt
     7021 catgagaatc gcaagatact ttttaatgga aaagagatac aagtgaacag cggagaattc
     7081 gtcacagggc gcgatgctat cacatctgag atgaacgaag gcgtgaaacg tgaacatcaa
     7141 gtgaacagcg gttctgtatg gagatggcta aaacaatttg aaaaaaacgg aatgttgaac
     7201 atcaaatcaa ctacgaaata cagcgttata tcaataaaaa actggtcttt gtaccaaagc
     7261 agtgaacaac aaatgaacat caaacgcaca acaagtgaac aacaagtgca cacaaacaag
     7321 aatgaaaaga attataagaa tgaaaagaat aataaaacat catcgtcgca accacgcaaa
     7381 aagcgtgttt acgacaccga ctcagtttac tacattctcg cggaggagtt attcaaacag
     7441 atttgtcaga atcaggaaat caaaaagccg aatctgcaaa gttgggcaga tgacattcgg
     7501 aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac ggggaatgat tgaatggagt
     7561 cagcacaatg tgttttgggc atcgaatatt ttatctgcta aaaaattgcg agaaaaatac
     7621 gacacaatgg cagcgcaagc aaatcgtgat tataaaacaa aacaaactaa aacgcttgaa
     7681 tacgagaaat ttagcacaga tgagttgcct atttgagagg aggcgtaggc atggaaactg
     7741 ttggggaaat catggaaaag ctgatacaga aagtgcttgt ccaacgtggc gaatgtcctg
     7801 aatgcggaca gcctttgtat ggatggcgta cgaagaaccc tgatggctca gaacgttgta
     7861 agccaacctg tatgcaatgt gggtataaag cattgcgagt tcaggaagac ttacaaacag
     7921 aacgaattta caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa ggtggttctg
     7981 ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa ttatcaaatt gtcgatcaag
     8041 aaacaagaca agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg ttaggaaatc
     8101 caagtcacct tgttttaact ggaaaacaag gaacaggtaa aagtcaccta gcgatggcag
     8161 cggcttggga agtcttgaag cgatcaaact acgataaaaa aatcttattt atcgggttac
     8221 aggaaatgct ggatcaaatc aaattttcgt acaacaatcc tgaactcaga aaaacgattg
     8281 agggatcgtt gattgcagat atcaaaacag cggatttagt catcattgat gatatcggtt
     8341 cagaactagg aaaagatgca tcagatagtc gagcgtttgg cataaacacg ctaaattcgt
     8401 tcttggatgc acgacagaac ctagcaacga ttatcacaac gaacttgctt ggtgaagaac
     8461 tgaaaaaagc ttacggtacg agaacaatat caagaatgtt tgtcaactct gatggattta
     8521 cgatggtatt ttctcaaaca gcagacaagc gcataaaacc agtcaaagga gcgatcgcat
     8581 gaatttgact aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa ttataggtga
     8641 tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg gttcggaaga taagtaagct
     8701 aattatttat cgggatacag aaagatttct cactgtttta ggtaacgtct acgagaatcc
     8761 agagttattg gaggtagagt aattgccaaa aatattagat gcatgttgcg gtagtagatt
     8821 gttctggttt gataagaaca attctgacgt aacatttatg gattgtcgtc agcagtatga
     8881 agaattatca acaggtcatg tgatcaatgt tgatccagat gtggtcgcag actttcgcga
     8941 tatgcctttt gaagatgatg tgttcgatat ggttgtattt gacccgccac acttaagaca
     9001 tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa ttagacgaac tttggccaga
     9061 agacatcagg caaggttttg cagagtgtat gcgagtttta cgaccttcag gaacattaat
     9121 ttttaagtgg aacgaggaac agataccact ttctgatgtg ttagaggcta ttggagaaca
     9181 gccattgttt ggtaataaac gaagcaaaac acattggctt gtgtttatga aaaaattcag
     9241 ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt attaaataaa tgttttggtg
     9301 gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat attgaagtat
     9361 tcccatacat caataacttt agctatgact cagatgatga atacacgaga tacacaggcc
     9421 aaaagctagg ttcgatggat tttatctact atttcaaaaa agacccgaaa atcgacaaag
     9481 ttacaggaat ttatagcgaa attgaccgac tgtacggaat tgctgatgat tccagttttt
     9541 caagtgattc aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa ctaggaagtg
     9601 aggccagtgg accatatgca agtcttaaag tcgttgatat acccgatggg gctgagtggg
     9661 aaataagtga ttatgacggc gttgaaactg cacattatgg ttttcaaaca ggtagtattt
     9721 agtccactaa tgacaactaa agaggaggca gaaggatgag tgaagatgaa tactttgaac
     9781 agtagtctat taacgacaga gatttttaac tggaaagggg tgtagtcatg gacgcatcgg
     9841 aagtagtaaa taaaattaat agtctcaaag aggttttcgg agacgtcgaa gtagtgataa
     9901 acgcacaagg ctatgacaac gaatttttta agcagattac cgacattagc attcagcaag
     9961 gaatggaaga cgaagacgga aactttatcg atgaagtagc cattttagtg acttgcgagt
    10021 aagtaattga cagctaaaga ggaggaagcg gaatgaaact aaaagacgga ttttactcca
    10081 gcggccacgg catcggcggt ttaatgctag atatgccgac aaagaatcct aaaacacgta
    10141 agaaaccaaa attcaaagtc ggtgacatgg ttcgctgcga agcagaagga ttcatctatc
    10201 catttcgtgg atatgtagaa cacgtctata atcactcagc aatcattcgc attgaaaaca
    10261 cgatggaatg tgataagtgg ttagcgaaaa gcaaagagaa tttagcagtg gtgagattgg
    10321 tggacatgga agttataaaa tcttaatttt gcttttttac aactatccta caacaatgag
    10381 cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga atatagatat
    10441 taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt tagaacgttt
    10501 ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt tgaaattcac
    10561 aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt cggttatcga
    10621 agcattaaca cggctaagta gaatccattt ccaggtactt tattatagtt attgttttcc
    10681 taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata gaactatcga
    10741 gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag gagaactcat
    10801 ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt tccgacataa
    10861 gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa gaaatatgag
    10921 acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc tgtgtttctt
    10981 tcagcatatc tatcagctag atgtagagta gggcaacatc gagaaccaaa agtgacagca
    11041 tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga aagaatgctg
    11101 atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt tgttcgaggc
    11161 tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa gaagatattc
    11221 tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa taatactagc
    11281 gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc atcgttagaa
    11341 ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta ccaatttggt
    11401 ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta ttatgatagt
    11461 gtcgaaaaat taggaaacag gatcgacaaa ataaaatgta agggaggaaa tctccctcat
    11521 cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat gtgaatttca
    11581 actccttcta aattgttctt attatctatc atccgttgct gtctattgtt tttttaatta
    11641 ttcacacgat aactaaaggt aagtgaagag aaatgattcc attaataatt tcaatttttg
    11701 cgctttgtct taatgtatat atgattggat ttaaaaatgg gcaaaataaa aaatagtagc
    11761 agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgc
    11821 taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagtgact ttggcaaccg
    11881 aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg
    11941 taggttgcta ttacataact ggttaggtta gattgagatt tgggattcgg tacaaatgaa
    12001 tcgtcaaatg tctcaagcac aggatcggaa acgtccctgc ctgtgcatta catattagat
    12061 cactctttga gtggtctttt tctttacata aaggaggcta cataatgaga aactactggt
    12121 atgtatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc
    12181 gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat ggggagagaa gcagaaccat
    12241 gtgagattga tgcgtgcaag ctcgtttatt gcggtcatgg tttctatgat gaaccaaaca
    12301 ttcaaaataa tattaacaag aatttgaggg attagaatgc aagaaatggc ttaaaaatct
    12361 ccaatcagga caaatcaagt tagacaaagt ttccgattta aagatattga ttgaagctga
    12421 tttaatgcta aaagatattg aaaattagaa aacaaaactc aacctaaaca gaatgtgagg
    12481 tggtgtgtat tgaatggcaa gacaacgtga tccaagacgt gatgaagcca aaagaatttg
    12541 gttagaatcc aacggagaaa agcagttaaa ggaaattgca tctgaattaa atgtttcaga
    12601 ttctcaggtt agaaaatgga aatcgcaaga caaatggagc gctgaattga ttagtaacgt
    12661 taccaatggc aaaagtaacg ttactaatca aggtggcgct cctattggta atcaaaatgc
    12721 taaaggtaac aaaggaaata gccgagcttc tccgccagtg ggtaataaaa acgctttgaa
    12781 aacaggtgag tatgaaacaa tattttttga cacgttaacc gataatgaga aggacatcta
    12841 ttctagtttg aatgatgatc cttcttttgc tttgtccgaa gaaatacgac ttcttaaaat
    12901 aagacaactt cgaatgatga aaagaatcca acaagctgaa gctggactaa atgatgaaga
    12961 agtcgaacga ttgcagcagc taagaaagat taaaactccg attgaaaaaa atggtaaaaa
    13021 gctagaaatc aagcgtgagg ttatgcaaga tgtgcagatt agcagaaaaa aacatcgcaa
    13081 aattgatgat attctttcaa ttgaagattc attgactcgg attagcaacc agttagctaa
    13141 agccatcaag caaatgaatg aactttatat gaatgaatac agaactgatt taattaaagc
    13201 tcagactgat aagatccaag ctgagacaaa tgaaattggc ggaaataatt caggtgaaga
    13261 aatagaagaa tggaaacagg cagttttaaa tgccgcaaac aaacgggcgg tgaaagaaaa
    13321 tgaataatga atttattcct tttgctgata ttggttctgc cattgattat tactatgata
    13381 aaccagtagc tttttgccaa gatattttgc atttgaatcc tgatgaatgg caagaaaatg
    13441 ttttaaatga tttagctgaa ttttcaaagg tttctgttcg ttctggtcaa ggagttggaa
    13501 aaacagcatt agaagcagga gcaatacttt ggttcttaac gtgtcgaccc tacgctaaag
    13561 taatagcaac agctccgaca atgaagcaac tttacgatgt actttgggca gaggtagcta
    13621 aatggttaaa tgatagcttg atcaaaaact tactgaagtg gacaaagacc aaaatttata
    13681 tggttggtga ttcagagcgt tggtttgcta cggctagaac agcgactaaa ccagaaagta
    13741 tgcaaggttt tcacgaggac catatgttga ttgtggtaga tgaagcttct ggtgtgtctg
    13801 atccaattat ggaagctatt cttggtacgc tatcaggttt tgataataag ctgttgatgt
    13861 gtggaaaccc caataatatt gaaggtgtct tttatgattc ccataattca gaccgtgata
    13921 aatacagagt tcataaagta tcaagctatg atagtaaacg tacaaacaaa gacaatatag
    13981 aaatgattct taaaaaatat ggaaaagaaa gtgatgttgc tcgtgtccgt atttttggag
    14041 aatttccgaa aggtgcgttg gattcattta tcagtcttga aacagttgaa ttggctacag
    14101 aaaaacaaat tagtgattct ttagtcaata aaacaaaggt tgctcatatt ggtgtcgacg
    14161 tagctcgata tggtgatgat tctacgattc tctttcctag aattgctacc agggcattgg
    14221 agtatgagaa gtattcaaaa cgtagcacca tggaaacaac aggatatgtc atcaacatgg
    14281 ccaagaatct aatgagtcaa tatccgagta ttgataaagt gatgattaaa gtcgatgaca
    14341 ctggtgtcgg aggtggtgta accgaccgcc tagaagaact tatagaagac aaacattatc
    14401 cttttgaggt gtttggagtg aataacggtt caacatcaga agacgatttt tacgataatt
    14461 taggtactca actatgggga aacatcaagg aaatgttaga agaaaatatg acagcaaatc
    14521 ttaacggaga acagcctgtt attgaattgc cttctgatag ttcgttaatc aaagaattaa
    14581 gtactcgcaa attcaaaatg acaagtagaa gtcgtatacg tttagaaagt aaagatgata
    14641 tgaaaaagcg aaatattggt agtcccgata ttgctgacgc actggcttta gcgttttatg
    14701 agccaccaag tcactatcaa tttattcaat tttaggaggt gagcttttga ttagtacagt
    14761 attatcttta gagagatata agaagctacg tgttaaatat gcaacacaaa ttgaagatgg
    14821 aatgtttgat ccgaatggtt ttatagaaga tatgaaacca ttttttgctg atcgcgaaag
    14881 aaaatatcta gcttatacta gcgaaaagaa tgaaatagat aatagaccaa agcctaacac
    14941 tgatattgta aaagttaata ataaacttca tgctggtatg tattcaattg tcgtagacca
    15001 agcagtcaat cattttactg gtatacctat caaatgggat tacgatgtat cggaacaaaa
    15061 aagaacactc attcaaagat taaaagataa attcttaaaa aatgatatag aacttccgac
    15121 agttccagaa gcttttaaca aattaacaag caatcttgat tccatgagat tcgcaatgct
    15181 tgattctgaa actgcaactt ttcaaggagc ttgtggagta gctttccgat tgctagagcc
    15241 tgtggaagaa gatgatggtt ggaagttaag agcaagtaat attgaacctt ggagagcaga
    15301 aagatatgga aatgccggaa tctatatcaa agaaaagtat gactcttacc agaaaaaatt
    15361 ttttcaagaa atgaaagtta tgacaagaaa taaaattctg acatatgctt gctatggtga
    15421 tttgaatttt gttacaagcg gaacgttcaa aaaaattgat gagaccgata accctttggg
    15481 gacgattacc ttggcagaat ttaaaaataa tacgaatcgt tattgtgatt ttgaagtagc
    15541 tgaggaaatt ggagatgcaa ttgatcgtgc tttgtctgat caacaaaacg aaatcgaaca
    15601 atttaagctt gcgtacatgc ttgttactgg cacaacaatg agcaaaggca ctgctaaaga
    15661 aatgatgaat cagctaggga ttatcaactt aaaagcccct actgcaaaag ctgaatatgt
    15721 cacaaaaaat ttagcaaaag atttcaatga atatcatatg gatctattga aaaaacagtt
    15781 ttacaccatt tgtaaagcaa tcgattttaa cgatgaggtg tttaaatcaa acagttctgg
    15841 agaagctcgc aagtggcaaa tcattagcct agaagcgaaa acaaatacta aagagcaata
    15901 ttttagagaa ggattgaaag aatgcgcaga aacaattgct gcttttctta agtttcatga
    15961 caaagtagaa attgagcctg aaaaaattat ttttactttc tctagatctt tgccaactga
    16021 tttaagttat ttagctgaag ctttacctaa acttgcacca tacgtatcca aacgaactat
    16081 tcagagccaa attccatttg taacagatgt tgattatgaa aatgaaatga tggaattgga
    16141 aagtggaagt gcttatccag atagtgaata caattttggc ggaggtggca atagtgacga
    16201 tagaaacgaa gtattggacc aaacgtcgag aactggagga tcaagcaagg ctcaaacaag
    16261 aaaatcagac acttaaaaaa ttaactagtg tatttcctga agcactgaaa gagatacaag
    16321 caaaactatt atcacaagct gacttacaca atatcactta tccagaaatg atggagtttt
    16381 acagtacaag taatcagaaa aaatatcgtg aatatgtgga aaaaaattat aagtcattaa
    16441 aaatgtatga tgcaaaatac aaagagttta tcgatgaatt ttttccacca tttgactatg
    16501 caaaagtcaa tcgcttatta caaatacgat cagatgtatt taaaattctt gcagaatatg
    16561 cgatggatgc agatgtgaat caatattttt ctgatcgctt agaggaaatt cttcaaagaa
    16621 catattcttc taatgctaat gtttttgttc aacttttaaa cgttgatata ccaaattatt
    16681 taccagaaaa tgaaattaaa cattatttga actatccatg gtcaggaaag acattttcaa
    16741 gaagactttg gggaaatatt tcatcgcttg aacagaaact ctctaatgct attgtaaaaa
    16801 gtgttgctag tggagaaggc gttatacacg cattaaacac catgagatta gactcagaaa
    16861 tttgtgacat gtttaagtta gaagaatcaa agtataacaa agcgatagaa aatctcgttc
    16921 gaacggagta tgcaaaattt gcacaagatg gtattgaaaa atcatattta gaaacaggta
    16981 ttgaggaata caacgtattg actgcaaaag atgagagagt ttgccggatt tgtggaggaa
    17041 aggcaagtaa gaatccctat aaactgaaag atgctgtcat aggtgaaaat cgagcacctt
    17101 tccatagtcg ttgtagatgt acggatgttc ctaatttacc aaaattagga aaggatattg
    17161 atgaagaata tgaccgttta tttggcgatt tattagatga gtttgcacat gattcttttg
    17221 gaattaattt gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt
    17281 aatgtaaatg ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct
    17341 aatggcttga gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact
    17401 gttaattatc aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta
    17461 ttatctcatt cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa gatgggatgt
    17521 attgttgtta gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt
    17581 ccgaaatgac gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt
    17641 aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgagcatg
    17701 aaacacaaat cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag
    17761 ttttcttttg atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt
    17821 cagtcacaat ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta
    17881 gataaaatta aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct
    17941 gagcaaattc aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg
    18001 acaagtgata ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag
    18061 ttaaaagagt ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc
    18121 actaagaatg ctgttgaagg ttttacaagt gttttgaatg caattaatga gaaacatgca
    18181 gatgcattaa aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac
    18241 caggttcagc agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt
    18301 caacaattta actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt
    18361 atttggatat ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct
    18421 atttacttgg tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat
    18481 atgatatcaa caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg
    18541 aatcaggtcg aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat
    18601 ctggtacatt agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag
    18661 cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag
    18721 ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag
    18781 caaataaggc agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat
    18841 ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata
    18901 tgcaggagtt aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg
    18961 aaagagtaac ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg
    19021 ctctactaga accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga
    19081 aactacgtcc attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac
    19141 gtatcggtct tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt
    19201 taaatttaaa gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca
    19261 gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact
    19321 aaaaattttg ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat
    19381 aacgaattta tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt
    19441 gatgttaatg aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg
    19501 agtctacaaa agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa
    19561 taattttgct agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt
    19621 taaaaagcgt gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag
    19681 tatcttcagt tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta
    19741 gtgagttact tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg
    19801 agttttttta atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat
    19861 tgaaagactg acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa
    19921 agtatatgat aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt
    19981 catagaagat ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc
    20041 taagattaaa ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat
    20101 tttttctcta tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat
    20161 aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga
    20221 attttaaaaa tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa
    20281 tgaataaagt cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc
    20341 gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagaagtta
    20401 taaacaatga acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct
    20461 ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc
    20521 ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat
    20581 gaaatcattg ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat
    20641 cttgattcag ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct
    20701 ggaactgaaa atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg
    20761 attgataata accctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt
    20821 tttaatacga ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa
    20881 gcagatggtg ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt
    20941 gaaagataaa tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact
    21001 tggtttcctt tggaagatga gacagggaat tttccggtat atggtactgc gttgccaatg
    21061 ggaacagcag taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat
    21121 tcagtggttc aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat
    21181 ggatatactc ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc
    21241 ttgcgttctg gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg
    21301 aatgggaaat accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa
    21361 acatcaacga ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt
    21421 gtagatgtac caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat
    21481 aaagcaatga tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt
    21541 tcaggaggta cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagacg
    21601 aagaaggtaa atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg
    21661 tagaagatgc tgcaggagtt atggaagaaa tgcaaacagc gactgataag caatcagtta
    21721 aaaaagcttt aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta
    21781 ctggtgaaga ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat
    21841 tattgaagtc tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag
    21901 tgaggcgctc aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa
    21961 gacgcaatta ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga
    22021 gttaatgcgt ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta
    22081 tgataatttc actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag
    22141 atgatactag atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac
    22201 tcttttgatg aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt
    22261 tggaaaatat ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc
    22321 tttgatctaa tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac
    22381 gctctacgaa atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga
    22441 aagaatgggc ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata
    22501 agtcatgttc aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt
    22561 aaagctttaa atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct
    22621 gttattcaat tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca
    22681 atgatcaacg ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca
    22741 atgggagatc tgaaagaagg tttatctcaa ggtaagattt ctgttgaaga attccaaaat
    22801 caattaatag aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct
    22861 aaagattcta ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt
    22921 ggtgttggtg aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga
    22981 tttaagggaa ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct
    23041 gcaaatattc ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt
    23101 ggttctgctt tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg
    23161 ttcatgtttc aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat
    23221 gccataattg gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaatcca
    23281 tttgttttaa ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca
    23341 accaatgaag agcttagaaa caaggttata tctatttgga atgatgtgaa agattccgta
    23401 attggtgtat taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa
    23461 atggcatcaa atgcagttga gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt
    23521 tctaacacct ggaaaggtat taaggatggg gccacaggtt tatttgataa aacagtagag
    23581 acttcaagaa atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt
    23641 tctgatactt ggcaaagtat aaaagattca gccagagaaa aatggaatgg aatcaaaggt
    23701 tctattatgt cagttgctgg tccattgatt acaggtataa agaatgcatt tttacatgtt
    23761 actttttatc tagagacctt atggaataat ttagttgaga ttggtaaaaa cgtctttgag
    23821 attttaaaaa atgttatact tgcgccggtt ttgtttatta cctccatgat ttctggtgga
    23881 tgggaagaaa caaaaaataa tatgattgct gtttggaata atattaaaga aagtgccata
    23941 aatatttggg aatctataaa gaatatattt gtaagttatt ttacaaatat ttattttgct
    24001 gcactcaata tttggacagg gttcaagctt acattgataa atatttggaa tgaagtggta
    24061 aatcaagcta aatcgatttg gttaaacata aagtattttt ttattaatct ttggattgat
    24121 ataaaatatt ttgcaattca gaaatggatt gaattgaagt tttctattat tcaaacttgg
    24181 atcgatttaa aatataatgc cattactact tggaataata ttaaacagtt tttcaaagat
    24241 acttggaaaa atattaaaga tacggcatac aatacgtgga tttctataaa aaataccatg
    24301 attaatacat ggaataacat taaggactct ttctggaata ttgttactgg aattgttaat
    24361 tctgctgaaa atgcatggac aaatctaaaa aatggtgttt caaatgctat taatcgggta
    24421 aaagaaatct ttaattcgtt aagggaagtc aatttatttg aaattggcaa gaacatcatt
    24481 gatggactta taaatggtgt aaaagaaaag tggaatgctt tgaaagatac tatcaaaggt
    24541 attgcaggta atatcaaaaa ttggattaaa ggagctttag atattcattc tccatcaaga
    24601 tggatgcgtg atatggttgg taaaaatatt gttcaaggta ttatcgttgg tattgataaa
    24661 gagcaaagta agttagatca aaccatgaca aatttagtaa aaacaccgtc tgttcaacct
    24721 gtagtaaatg gaataagtca cctaccaatt acacaaacaa agcaaaatat atctgctagt
    24781 gaaacaaaag aaattcattt acatttaaat gtttatggag atttacctga ttcaatggtt
    24841 cggaagatta ccacaaaaat taaaacagaa ttgacaagac aaatgaaacg agatgctggt
    24901 gcagtaggag gaacattata tgcaacttaa gagaggacaa ttttttatta atcaacatta
    24961 ttcttctgaa tttaatgtgt atattcaaaa caggcctgct tctgtttcag ctagtcgagt
    25021 aattgaattg agagagcgag aaggaaatga ttctatcatt attgataaag gctattacaa
    25081 aaatgtgact agaaagattg agtgttacta taaagcacct tcaattgata tggtgcagga
    25141 gtgggaagat cgaatcactg aatggttaga tatgtcgtct tatagtgatt ttattcttta
    25201 ctatgatgaa caatatattt atcaagctgt agtaatagaa ggacctgaat ttaaaggaac
    25261 aagaaaaaca ggaaatattg tcccttttga atttacagtg agtattcgac catttaagga
    25321 aaattacaat ggtagattta ctattcgaca aacagaaact tttgaaattt ataacccaga
    25381 gaagtattct tcaaaaccgc ttattaaatt gagtggttct ggagatgctt ctttttatat
    25441 taataaggat aaatatgatt tgaaatcgtt ggacagagaa ttatttatag attctaaatt
    25501 agaagaggct tatcgaaaat tagatggtaa tttagaacat caggatcagg tcaccttatt
    25561 tttagatttt ccatttttat atcctggaaa aaatgaaatt aaatggacga aaaatattca
    25621 ttcatttgaa ataatgccta ggtggtggag aaaagtatga aaccaagaat atatagtcct
    25681 actgaaacag atttctcaac gaatggctta ggaattttaa aggattgtac aagatgtgga
    25741 atatatgaag tagctaatgg aaaatatgaa ttagagttgg attatccttt aggaactaga
    25801 tttgatgaat attttgaaaa tgactatcaa ataaaagcaa agccaaacga tcaagaagag
    25861 tatcatatct tttttattga tgataaagat atagatactt ttttaaatac agtaactatt
    25921 tatgctcaga gtcgtacaaa tcgacttgga agacgggtag tcactcttgt ggaagtggac
    25981 tctaaaactg gtcaagaagc gatatcaatt attgaaacta aaatggataa aaaatctgac
    26041 atacgacttt attctgatat tacggctgtt tctagcacaa tctttgaagc gagaaatgtt
    26101 ttaaattgta ttgctggtga acaaggatca ttgcttcaat attggggcgg agaaattaaa
    26161 cgtgaaccat ttaagctttc tttgttaaag cgaagaggtc gcaataatgt tggaacgatt
    26221 cggtatggaa aagatatgtc tggtttgaag gtcaaattag attggacagg tataaaaaca
    26281 agaattattc catatgctga tcctcaaagt gaagtaggta cgactaatcg aatttatggt
    26341 tcgccagtag atagtgccta tattaataac tatcctgatg tctatacaga acatgttcag
    26401 tttacagaag aacaaggagt aaaagacatt aagagcttaa ataaaatagc taaaaattac
    26461 tttaaaacga tcaatcctgg ctgtgataag cctaaagttt ctattacggt tgaatttgat
    26521 aagttgactg atagtgaaga agcgaaagaa tttgcgaaga ttagaaatta tggtctgttt
    26581 gatacattta aaatatatca taaaaaatat aatatttatc ttgaatcaaa ggttagtgga
    26641 ctacaatacg attctttatc tgaaaaaact ttgaaattag aagcaggaga cattcaagtt
    26701 gctttttatc agcaacaagc tgtaactatt caggataaat taaaagatta tgcaacgaat
    26761 aattatatga gtgattttaa tgattatgtt tcttcaatga ttacaggtca aggcagtgca
    26821 ggaggatatg tagttttatg gccaaaagaa aaaccttcca acatctttat tatggataat
    26881 ccagatttag aaaaggcaag agaagtacta agaatgaata aaaatgggat tgctttttct
    26941 aagaaaggat ggcgggggcc ttttaattca gcttggacat tagatagtat atttaatgcc
    27001 aattttatac aaactggatt aatcaaagca gatatctttc aaaattcttt taacaagact
    27061 ggagatgtat tgaaattagt taatggacta ctccaaattt ggaacaacaa aaagaaaata
    27121 atggagttaa ctaaaaaagg aatggaattt tggaattcta agggttcaat tggaacgatt
    27181 ggaacaactg attctgttgg taatcctttt cctggggctt ctactccaac ccctattgaa
    27241 gataattctt tagttattcg tacaaatgga gacggcaaat atattttgat ttctcctaaa
    27301 gtaggaaaag gattagtttt attaggaaat ggtaaagcaa tttattttgg agacttagat
    27361 gtacaaggaa aacttacagt taaaggaaaa gaaataattc ctggacaagg tggtggtcca
    27421 tcgggaggtg gagaatctac tggtgggtat cctagtgaat taaaaacaga tgcagaaaag
    27481 agagcttgga gaat
//
LOCUS       DASJON010000012        26763 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJON010000012 DASJON010000000
VERSION     DASJON010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.012"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..800)
                     /locus_tag="U3989_000403"
     CDS             complement(<1..800)
                     /locus_tag="U3989_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1713874.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKV"
     gene            complement(1024..2964)
                     /gene="thrS"
                     /locus_tag="U3989_000404"
     CDS             complement(1024..2964)
                     /gene="thrS"
                     /locus_tag="U3989_000404"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN1713875.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3301..3480)
                     /locus_tag="U3989_000405"
     CDS             complement(3301..3480)
                     /locus_tag="U3989_000405"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN1713876.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3527..5350)
                     /gene="cadA"
                     /locus_tag="U3989_000406"
     CDS             complement(3527..5350)
                     /gene="cadA"
                     /locus_tag="U3989_000406"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1713877.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5628..7907
                     /locus_tag="U3989_000407"
     CDS             5628..7907
                     /locus_tag="U3989_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1713878.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8000..8647)
                     /locus_tag="U3989_000408"
     CDS             complement(8000..8647)
                     /locus_tag="U3989_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713879.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8826..9545)
                     /locus_tag="U3989_000409"
     CDS             complement(8826..9545)
                     /locus_tag="U3989_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1713880.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9676..10827)
                     /locus_tag="U3989_000410"
     CDS             complement(9676..10827)
                     /locus_tag="U3989_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN1713881.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11044..11784)
                     /locus_tag="U3989_000411"
     CDS             complement(11044..11784)
                     /locus_tag="U3989_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1713882.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11788..12111)
                     /gene="rsfS"
                     /locus_tag="U3989_000412"
     CDS             complement(11788..12111)
                     /gene="rsfS"
                     /locus_tag="U3989_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN1713883.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12168..12761)
                     /gene="yqeK"
                     /locus_tag="U3989_000413"
     CDS             complement(12168..12761)
                     /gene="yqeK"
                     /locus_tag="U3989_000413"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN1713884.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12745..13395)
                     /locus_tag="U3989_000414"
     CDS             complement(12745..13395)
                     /locus_tag="U3989_000414"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN1713885.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13405..13716)
                     /gene="yhbY"
                     /locus_tag="U3989_000415"
     CDS             complement(13405..13716)
                     /gene="yhbY"
                     /locus_tag="U3989_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN1713886.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13732..14841)
                     /gene="yqeH"
                     /locus_tag="U3989_000416"
     CDS             complement(13732..14841)
                     /gene="yqeH"
                     /locus_tag="U3989_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN1713887.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14838..15365)
                     /locus_tag="U3989_000417"
     CDS             complement(14838..15365)
                     /locus_tag="U3989_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN1713888.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15545..16330)
                     /locus_tag="U3989_000418"
     CDS             complement(15545..16330)
                     /locus_tag="U3989_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN1713889.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16323..17192)
                     /locus_tag="U3989_000419"
     CDS             complement(16323..17192)
                     /locus_tag="U3989_000419"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN1713890.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17193..18572)
                     /locus_tag="U3989_000420"
     CDS             complement(17193..18572)
                     /locus_tag="U3989_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN1713891.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18580..18999)
                     /gene="fabZ"
                     /locus_tag="U3989_000421"
     CDS             complement(18580..18999)
                     /gene="fabZ"
                     /locus_tag="U3989_000421"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1713892.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19024..19500)
                     /gene="accB"
                     /locus_tag="U3989_000422"
     CDS             complement(19024..19500)
                     /gene="accB"
                     /locus_tag="U3989_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN1713893.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19503..20738)
                     /gene="fabF"
                     /locus_tag="U3989_000423"
     CDS             complement(19503..20738)
                     /gene="fabF"
                     /locus_tag="U3989_000423"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN1713894.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20757..21494)
                     /gene="fabG"
                     /locus_tag="U3989_000424"
     CDS             complement(20757..21494)
                     /gene="fabG"
                     /locus_tag="U3989_000424"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN1713895.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21506..22423)
                     /gene="fabD"
                     /locus_tag="U3989_000425"
     CDS             complement(21506..22423)
                     /gene="fabD"
                     /locus_tag="U3989_000425"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1713896.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22530..22754)
                     /locus_tag="U3989_000426"
     CDS             complement(22530..22754)
                     /locus_tag="U3989_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1713897.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22807..23769)
                     /locus_tag="U3989_000427"
     CDS             complement(22807..23769)
                     /locus_tag="U3989_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN1713898.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23779..24219)
                     /locus_tag="U3989_000428"
     CDS             complement(23779..24219)
                     /locus_tag="U3989_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1713899.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24525..25490
                     /locus_tag="U3989_000429"
     CDS             24525..25490
                     /locus_tag="U3989_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1713900.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25547..25777)
                     /locus_tag="U3989_000430"
     CDS             complement(25547..25777)
                     /locus_tag="U3989_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN1713901.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25863..26750)
                     /locus_tag="U3989_000431"
     CDS             complement(25863..26750)
                     /locus_tag="U3989_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN1713902.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
BASE COUNT         7394 a         5906 c         4632 g         8831 t
ORIGIN      
        1 actttttcgc taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta
       61 gcaaattggg ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta
      121 gatgtttgta cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac
      181 gcaatgattt cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt
      241 ctacctgagt ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg
      301 agcaagacgt ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc
      361 agaatatctt ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc
      421 gattccaaac actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct
      481 tttatcgcta cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt
      541 gcttctatat cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt
      601 ttgaagtccg tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca
      661 ctcaacccgg aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta
      721 ccgataatgt agttggccaa ctttttttct gctgaagaat aatcttccat catactttca
      781 atacgactaa ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata
      841 ccatatcaga cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc
      901 aacagaatat cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga
      961 atacctgtta tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac
     1021 ctattagttt tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt
     1081 caaatcttct acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc
     1141 caattccttg tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat
     1201 cttatagccc attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa
     1261 acgttctttg atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt
     1321 tgcttgtata ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc
     1381 gacaaaacgt tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt
     1441 ttccccgtct tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg
     1501 tatagttgat aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg
     1561 tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc
     1621 ggctttcagc attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt
     1681 attgtttgga tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata
     1741 tactgccacc atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac
     1801 aaatgtatga ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc
     1861 tgatttttca tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata
     1921 agaatggatc gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac
     1981 aagcatttcg ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca
     2041 gtgtccagat gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta
     2101 acctagacta acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc
     2161 ttttggaagc cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag
     2221 ttcttttcct agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc
     2281 tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt
     2341 tgagtttcca cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc
     2401 agtggatgga acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt
     2461 aatcacttct tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa
     2521 aatttctaat gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac
     2581 gattttcatc atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc
     2641 attatctgta tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt
     2701 tggatagagg cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc
     2761 aagtgcatct tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg
     2821 agtcaaatca acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt
     2881 tgaaatgctt tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc
     2941 atctggaaaa gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga
     3001 tcatcaagct gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa
     3061 agttcgtggt tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc
     3121 gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa
     3181 agtttcagcc tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat
     3241 caatcaatta tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac
     3301 ttattttttc cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac
     3361 atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat
     3421 catattttct aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat
     3481 aatgctctct cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg
     3541 tcttgggtac tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa
     3601 tagagaccgt atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg
     3661 tacttgcaat taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg
     3721 ctattctcat cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt
     3781 tcaagatgac cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa
     3841 taccgatatc tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa
     3901 caggtctttg atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac
     3961 gggcatgtac agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat
     4021 ccccggtgat catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg
     4081 ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg
     4141 ttacatagat tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc
     4201 caatctttac ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca
     4261 gttgactaac aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga
     4321 tatggctaga ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc
     4381 cattgactgg ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat
     4441 caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga
     4501 tcccgttttt acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca
     4561 gtggacaagg tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc
     4621 accaagaaat accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag
     4681 ctaaacggac aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt
     4741 gatactggct gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta
     4801 aagaccctga cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca
     4861 aagaagattc atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt
     4921 cgccaggctt gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca
     4981 attttccttc tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt
     5041 ctttacccgc tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca
     5101 atatcatcaa actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg
     5161 ctaatacatc tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca
     5221 agaaggcgag aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa
     5281 aaacaaattc acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga
     5341 aatgtgtcat tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc
     5401 agctgcctct ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt
     5461 aattgaaaac aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat
     5521 attcagcaag gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta
     5581 tgctatactt ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac
     5641 aactagaaca aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc
     5701 tggatacaaa acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta
     5761 atcacaagat tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc
     5821 aacatgagca ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc
     5881 aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc
     5941 gtgaaacaga aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa
     6001 tcgttattga ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg
     6061 cattttatga aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta
     6121 aaatccaaga aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact
     6181 ttttgctaga gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga
     6241 tacaaaaagc acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag
     6301 gaatcgccgg aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc
     6361 ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt
     6421 ctgattacat ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat
     6481 tcaaaaatta tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag
     6541 aaagtggttt tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct
     6601 tagtcgatca gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg
     6661 atatgaaaat aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag
     6721 aaacaaatga acaattacct ttacatcata gactttctct attacaaacg aagctcctga
     6781 aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg
     6841 aagaacagct ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg
     6901 agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc
     6961 atcgagggat catgaattat caatttgtca atataacgaa acaatatctc cactttctac
     7021 aaactgtgcc actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac
     7081 atttgacaac tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac
     7141 tattcttttt actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata
     7201 tctttattga tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt
     7261 tcccaaaagc aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa
     7321 cgatcgttgg atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa
     7381 cgacaagcta tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag
     7441 aacatcaagt agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa
     7501 cagatgatat caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc
     7561 ggtattggcg tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc
     7621 aactgccaga cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga
     7681 agcgttccat tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt
     7741 tcttgtggga tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca
     7801 ccatgtgtac acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt
     7861 tgttaaacaa tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac
     7921 tgctatcatt aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa
     7981 ttcaatcatt taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta
     8041 atttgacaag cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa
     8101 ataccttgct atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt
     8161 caatgaattg gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct
     8221 ctgatacacg aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct
     8281 tctcatttct ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta
     8341 gaaagaaagc ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac
     8401 gaaaaagcag cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca
     8461 tacactctaa ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt
     8521 caacgccgaa tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta
     8581 actcttctac tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg
     8641 cttccatatt cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat
     8701 gctctcatca taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac
     8761 aatctgttag tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg
     8821 gttgcttagt cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc
     8881 agtgcatcaa tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt
     8941 tgagggatca tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct
     9001 tcttgtatac tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc
     9061 attacatcgc gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct
     9121 tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca
     9181 cccatgttcc cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca
     9241 ttgttcgtca atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc
     9301 aattcagagt atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata
     9361 tgttttggta cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca
     9421 tgcggatcag gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa
     9481 atccggctat tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt
     9541 cccatatttt cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc
     9601 attatacttg tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca
     9661 tttttagtca attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt
     9721 gcctcccaat tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt
     9781 ttttccgatt cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc
     9841 atcggtaaat ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt
     9901 tttgatattc agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt
     9961 tgtttttacc tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat
    10021 gcgcgattct attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga
    10081 taacaaccga taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga
    10141 aagatcttct tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc
    10201 atggcgaatg gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc
    10261 tgcactttta cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt
    10321 tgcataactt aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata
    10381 cagactgcga aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc
    10441 ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta
    10501 atcaaacgaa gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa
    10561 gatttttact gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag
    10621 ctccactact aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat
    10681 gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc
    10741 tcctgttatt tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta
    10801 ctctacgata atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag
    10861 aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat
    10921 tctcgcaaac acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg
    10981 ttcggatata taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa
    11041 ccgctagtct ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc
    11101 tgtaaaatca gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata
    11161 actttccaat ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga
    11221 cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc
    11281 tggataacta tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg
    11341 aaaaacctca tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc
    11401 taacgcttga tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca
    11461 cagcgaatca gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat
    11521 attgccttcc acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat
    11581 caacatctct tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac
    11641 cgctaatgct ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg
    11701 acgtttcgaa aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat
    11761 gaaagcaaat gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg
    11821 agcatctgac catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg
    11881 tacgatcaca tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg
    11941 ttttacttca tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat
    12001 ttgacgttcg ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg
    12061 aacgtctaaa gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt
    12121 taattgttct ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct
    12181 acccagtaat tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa
    12241 tgggccaatg tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg
    12301 gctcgtgcat cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa
    12361 atgattttgt caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga
    12421 agaatctctg catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca
    12481 tgccagattg cattattata attcaataac tccggatcaa aaccgtctct tacgattacc
    12541 attttgaact cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta
    12601 gctttttcag gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca
    12661 agcacatgct tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct
    12721 ctagaataag cagtatattc attactatag tccatattca tacaaccctt cattttgaat
    12781 ataatcaatt actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg
    12841 acgtattttc gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg
    12901 agtatccgtt gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc
    12961 atcgatttta taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata
    13021 atctgtatcc ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc
    13081 accacgagct aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc
    13141 caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg
    13201 ataagaaggc atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat
    13261 cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt
    13321 cttctctaag aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc
    13381 ttgcgtattc atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata
    13441 cttttctttt gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc
    13501 gcaatctatt tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg
    13561 aagtaacgat actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat
    13621 tgctgaattg atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct
    13681 acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa
    13741 tgctttgcgt ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc
    13801 tgttacagtg atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa
    13861 ctcaaaacga acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa
    13921 tccacctaca tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt
    13981 tacatcattt gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct
    14041 tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat
    14101 ttcttttaac ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg
    14161 gcgatggata attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat
    14221 tttatccaag gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt
    14281 atttttgatg atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata
    14341 gacatcccgt ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga
    14401 tttcttagca ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc
    14461 cttcatccat tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt
    14521 cccaactaaa agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc
    14581 attgaagtca aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt
    14641 cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct tgggtctcat tataatggcg
    14701 taatctgaaa caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt
    14761 ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat
    14821 acattgtaat tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct
    14881 aatagatagg ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct
    14941 gttgcgataa tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata
    15001 tcagtcataa tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc
    15061 tttgctatgt tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca
    15121 aatttttcaa tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg
    15181 attcctgcat ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta
    15241 ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt
    15301 aattgggcag gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa
    15361 aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg
    15421 tattatactg catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag
    15481 catttcttta caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa
    15541 acaactaata ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc
    15601 gaacaagttc cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc
    15661 gtggcaactg ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc
    15721 gtagttctgg cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt
    15781 tccacaaaat cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca
    15841 tccagacttc atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga
    15901 tgatggcgat gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct
    15961 ctccttcgcc gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg
    16021 taatcaccgg tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc
    16081 cttcaggatg gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt
    16141 gaataccgat cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt
    16201 cgtcatctgc aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa
    16261 aaaaatccaa gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg
    16321 tcttatccac gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc
    16381 tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca
    16441 gccttttgga aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca
    16501 gcaaaaccga tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg
    16561 gcagttactc ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg
    16621 ctatgccgtt tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct
    16681 tgcatccgtg cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct
    16741 cgttcgaata atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa
    16801 ttaggatcca tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg
    16861 actgcttcat gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga
    16921 aatgctagcg gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca
    16981 ataatcaaag ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat
    17041 actttttctt caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc
    17101 cacatgttat caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc
    17161 cgaatatatt tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt
    17221 gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact
    17281 gcaggatgac taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata
    17341 aattcactta aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg
    17401 attatttttg caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca
    17461 ctgtctactc gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga
    17521 gaaggagcaa aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct
    17581 tggaatacta cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt
    17641 ttttttacta aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga
    17701 gtattcattt ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg
    17761 gcattttcat aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt
    17821 tttgtttgtg agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt
    17881 aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga
    17941 acttctatat ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat
    18001 gccgctgctg cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga
    18061 actttccgaa tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca
    18121 attctgtctg ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga
    18181 atgactggga catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca
    18241 tcgatcgttg cacttttagg accaataaat gtgatattgc actcttcaca catggaggca
    18301 aatcggctat tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg
    18361 attgcagcgc tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca
    18421 atgcagattg cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat
    18481 atcgctacgg tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca
    18541 ccgcggttag ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa
    18601 ttccgcttcg gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga
    18661 agctttcact ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt
    18721 acggaatttt gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag
    18781 agaaagtaat gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat
    18841 aacaggttct tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt
    18901 agcaacgatc cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata
    18961 acgatgtgga atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt
    19021 tgcttatttt tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc
    19081 tacaaggatt tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat
    19141 cgcttcaata atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc
    19201 tggtttgtcg ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat
    19261 tccatcaaat actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc
    19321 aggtatgaca ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat
    19381 gcctcgactt gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa
    19441 ttccgttagc gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa
    19501 aattattcct cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa
    19561 ttactcattg cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca
    19621 atctcaggat cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc
    19681 agcaaagttg ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat
    19741 ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt
    19801 gcttctgcct ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt
    19861 tccggctgta caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca
    19921 tctggagtag gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct
    19981 ccatagattt tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct
    20041 gctccttcac ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta
    20101 ggatcagttg attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa
    20161 atcgttgctt ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg
    20221 aatgcttccc caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata
    20281 ccttttgcac ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt
    20341 acgaacattg gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg
    20401 attgttggaa gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt
    20461 ttttctttat ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc
    20521 gaaaacatat ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct
    20581 ttgacttcac ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg
    20641 atgccattcg tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt
    20701 gaagtgactc catagccagt aattaccact cgattcattt gtttgctctc cttttcttat
    20761 ccgtgcatga ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt
    20821 gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt
    20881 tgcttttccg cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg
    20941 aatcctggcg caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat
    21001 tttgttaatc ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca
    21061 atcaacccag atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt
    21121 ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg
    21181 aaatcttctg catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact
    21241 aacacatgga tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca
    21301 taatcagaga tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct
    21361 atcttttctt ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca
    21421 aaagctaagg ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt
    21481 ccttttacat ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct
    21541 tattctcaac acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata
    21601 cttttccggg gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac
    21661 tctcatagaa acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt
    21721 tcttcattat ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa
    21781 tctgacttag gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag
    21841 gcccgctgac atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag
    21901 cttcgtctac tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg
    21961 caggagatac gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg
    22021 gcgcattcat cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag
    22081 cgcctctttt tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta
    22141 atgccgaata ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct
    22201 tcaataagcg ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct
    22261 gatccaaacg ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac
    22321 ttgcttcatc aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt
    22381 gatattgtgc accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat
    22441 ccagaatata tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa
    22501 tgtcccagcc cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact
    22561 aaatcttgaa ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca
    22621 atttcattga tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca
    22681 gtagtcaatt tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt
    22741 ttttcgaata ccataataat atattcctcc gatattgttt gctttttatt tttataattt
    22801 aacagtctac aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa
    22861 tattactttc tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa
    22921 gatagggatg cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc
    22981 ttgcggcatc tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa
    23041 aagcagataa tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg
    23101 tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc
    23161 ttctgaatag aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt
    23221 accgtcagct gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat
    23281 cccacctgct ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt
    23341 agatagtgtt tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc
    23401 agccatgctt aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc
    23461 atgagttgct cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg
    23521 cgacattgtt gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat
    23581 caatttttcc gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg
    23641 ttcacgaata cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag
    23701 atcattatta gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa
    23761 ttgttccatt aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc
    23821 atccagcaag gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc
    23881 acgatgaaaa tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt
    23941 tacaacgcgg cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt
    24001 gttaatagca gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt
    24061 tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc
    24121 tttgaattga gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact
    24181 aacaagataa tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag
    24241 tttgattatc aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct
    24301 taaaagatca tttttttact atatgagctt tttttaagat tttactcatt taaaacaact
    24361 gataaatcag catttataat ttcttttatt agaaaaatca tctatctcac atattttgat
    24421 tatcaaagta ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca
    24481 aaatttgtta tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa
    24541 tgaaaactct ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca
    24601 tctatgttgg taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata
    24661 aacagcattc aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg
    24721 tagtagacac ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc
    24781 ctttttataa ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata
    24841 agggatttgt attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata
    24901 aaaaaatgcc gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg
    24961 aaattagttt gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac
    25021 agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact
    25081 tgaaaaaaag acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc
    25141 aaaaagatat cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt
    25201 taagaacacc atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc
    25261 actacctcgt ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc
    25321 ctgacttcta ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag
    25381 aagaaatcga tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt
    25441 ttgaaattga actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg
    25501 gcttctttta ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt
    25561 catagatttc catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg
    25621 tttcagttaa ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc
    25681 cttctttttc aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca
    25741 gtcgttttat gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt
    25801 ctgttatatt gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt
    25861 ttttatgatt ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg
    25921 gttccgtcat tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta
    25981 gttgctggca atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg
    26041 ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa
    26101 ttgcttaaag accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa
    26161 tcaagtccgt ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca
    26221 atcatgatct gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta
    26281 aatgtcgtac caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca
    26341 ccgaccatga tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca
    26401 tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt
    26461 gtacttgtag tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac
    26521 tgtccatagt agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta
    26581 ctttgttctg ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga
    26641 tcagcttttg cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg
    26701 cgtgcgacag cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc
    26761 tcc
//
LOCUS       DASJON010000013        26708 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJON010000013 DASJON010000000
VERSION     DASJON010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.013"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            350..2044
                     /locus_tag="U3989_000432"
     CDS             350..2044
                     /locus_tag="U3989_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN1713903.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            2732..4609
                     /gene="ltrA"
                     /locus_tag="U3989_000433"
     CDS             2732..4609
                     /gene="ltrA"
                     /locus_tag="U3989_000433"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1713904.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4740..5117
                     /locus_tag="U3989_000434"
                     /pseudo
     CDS             4740..5117
                     /locus_tag="U3989_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            5117..6322
                     /locus_tag="U3989_000435"
     CDS             5117..6322
                     /locus_tag="U3989_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1713905.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6338..6982
                     /locus_tag="U3989_000436"
     CDS             6338..6982
                     /locus_tag="U3989_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713906.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6993..7799
                     /locus_tag="U3989_000437"
     CDS             6993..7799
                     /locus_tag="U3989_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713907.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7821..8051
                     /locus_tag="U3989_000438"
     CDS             7821..8051
                     /locus_tag="U3989_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713908.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8597..8872
                     /locus_tag="U3989_000439"
     CDS             8597..8872
                     /locus_tag="U3989_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713909.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            8914..9336
                     /locus_tag="U3989_000440"
     CDS             8914..9336
                     /locus_tag="U3989_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713910.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            9355..10947
                     /locus_tag="U3989_000441"
     CDS             9355..10947
                     /locus_tag="U3989_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713911.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            10959..11294
                     /locus_tag="U3989_000442"
     CDS             10959..11294
                     /locus_tag="U3989_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713912.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            11441..11635
                     /locus_tag="U3989_000443"
     CDS             11441..11635
                     /locus_tag="U3989_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713913.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            11667..14039
                     /locus_tag="U3989_000444"
     CDS             11667..14039
                     /locus_tag="U3989_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713914.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            14100..15560
                     /locus_tag="U3989_000445"
     CDS             14100..15560
                     /locus_tag="U3989_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN1713915.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            15571..17775
                     /gene="topB"
                     /locus_tag="U3989_000446"
     CDS             15571..17775
                     /gene="topB"
                     /locus_tag="U3989_000446"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1713916.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            17791..17940
                     /locus_tag="U3989_000447"
     CDS             17791..17940
                     /locus_tag="U3989_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713917.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            18014..18607
                     /locus_tag="U3989_000448"
     CDS             18014..18607
                     /locus_tag="U3989_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN1713918.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            18623..19522
                     /locus_tag="U3989_000449"
     CDS             18623..19522
                     /locus_tag="U3989_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN1713919.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            19525..20010
                     /gene="ssb"
                     /locus_tag="U3989_000450"
     CDS             19525..20010
                     /gene="ssb"
                     /locus_tag="U3989_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1713920.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            20376..20636
                     /locus_tag="U3989_000451"
     CDS             20376..20636
                     /locus_tag="U3989_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713921.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            21077..21283
                     /locus_tag="U3989_000452"
     CDS             21077..21283
                     /locus_tag="U3989_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN1713922.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            21283..21534
                     /locus_tag="U3989_000453"
     CDS             21283..21534
                     /locus_tag="U3989_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713923.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            21549..21986
                     /locus_tag="U3989_000454"
     CDS             21549..21986
                     /locus_tag="U3989_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713924.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            21979..22686
                     /locus_tag="U3989_000455"
     CDS             21979..22686
                     /locus_tag="U3989_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713925.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            23067..23246
                     /locus_tag="U3989_000456"
     CDS             23067..23246
                     /locus_tag="U3989_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713926.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            23587..23850
                     /locus_tag="U3989_000457"
     CDS             23587..23850
                     /locus_tag="U3989_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN1713927.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            23844..24194
                     /locus_tag="U3989_000458"
     CDS             23844..24194
                     /locus_tag="U3989_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1713928.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(24218..24832)
                     /locus_tag="U3989_000459"
     CDS             complement(24218..24832)
                     /locus_tag="U3989_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1713929.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            25146..25568
                     /locus_tag="U3989_000460"
     CDS             25146..25568
                     /locus_tag="U3989_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713930.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            25578..25781
                     /locus_tag="U3989_000461"
     CDS             25578..25781
                     /locus_tag="U3989_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713931.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            25992..26576
                     /locus_tag="U3989_000462"
     CDS             25992..26576
                     /locus_tag="U3989_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1713932.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         9923 a         4271 c         5480 g         7034 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaccactc tacaaaaaag aattgatcaa gattctgtat cgaatgaaca atatgaatga
      181 tgagtaggaa tggagaagag agaatgaaat atccgttgat atggttagaa aaagatgttc
      241 cgaaggaaga gaccactaga aaatttcctc acactttaag cctgggtaag ttgggaaaag
      301 ggcaatcttt taaccaagta aagaatcaaa caagaagagg tgactgtaca tgttagaagc
      361 aattaaaaca gctttttcac cacaaaatga tcaaaaagta cttgatccat taaagcaact
      421 aggaaatcgg gaaatagaaa ttgtaaaatc caaagggtat gatttagatt ttttaaaaca
      481 agtgatgcca caaggtgggt taaattttga tgaagaatat gtgacgtatg gtaatggtta
      541 ttcacgatgt gtgacagttt atcactacac aaaaaatcct acgctttttt ggttggccaa
      601 tctcatgaac aatcctcata cagtggccac actcgatgtt agcacagatg aaaagcaaaa
      661 agtgattgat caaatcaatc gtgccttaga tgaattaaat gatcgatcag ataatgaacg
      721 aaaagcgact gaccgaaata aaagcgcgga agaatacctg gaactagcgg aatatgctag
      781 aaaattgact tctaatgggg aaatttctaa gcaagtcaaa gtgagagtac atgtttacgg
      841 aaacacgatt caagaagtag atgaacgagc gcaggaactg atcgaaaatc tcaaagggat
      901 ggattatcgt gcggtgactt atctgcataa aaccaaacga gattggcaat cgatgtttac
      961 catgttcgat gaacaagctg aatggttcgg aaatatcaaa gggcaatcac taccatcgtt
     1021 aaacattggc ggaggtttcc cgttccacca ccaaacgcta aaagatccag gagggacaca
     1081 atatggggaa accatgacag gcggcccatt tgtctggaat gcgacgtata aaaatagtgt
     1141 acggttgtct actaatacct tgcttctagg aatgatggga gctggtaaat cgaccgttct
     1201 taaaatgatc gcggaagccc atttagctgc aggagacttt atttggggct ttgaaaaggg
     1261 aaaagacttt attcctttct taaaggaata taacggtatt atggtgcgtt tagacggatc
     1321 tgacggaatg atcaatcctt tagagatatt tgccacacgg acgtatgatg aagcatccag
     1381 tttatatgac gatggatcag tcaaagattt gaagatcaat gaagcagcgt cctatcaaac
     1441 acacttagac aaagtggtct atcaggtaca gttagtgagt cctcagctaa aaggaacgat
     1501 gaaagcagaa tttaagacct acttaaatcg tttttatgaa gagtatggaa cagtaccacg
     1561 cggctttact tcctcaaatt cgcgttcaaa tactgatacg caagtcacag ggaaagatcc
     1621 agaagcttat ccgacgttca aagaattttt agacttttta ggacaactgg aattaccagg
     1681 tgcttcccaa gagaaaaaga atcgaaaaga agaaatggaa tccatcgtag aatccttatg
     1741 tgagacgtat ggcatgattt ttgatggtca tagtacgatt cgacaccttg accaacaaca
     1801 gttggtcttt tttgacattg actctattac aggcttagat cgaggggtac aacaatgtca
     1861 aatgtacatg gctatgacgt tgatttggaa tcaggcattg attcaaggaa gaagacaacg
     1921 acgtttaatt aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat
     1981 tgatgagtgc cataatggtg cgacctgttc tgcagtgaaa agctgtgggt tgcaagtagt
     2041 ctaaaaacta ttgtaataac tcttataacg agaagtcaaa tgagtgggta acgccacaaa
     2101 gggcttccac tgatactccg acatgcaaca agtagtttat gcagaccaac gttgtgtgaa
     2161 gctcggtgaa gtcggctgaa ggataccgta aggtaaaaga aatagtgaat accacgaaag
     2221 ttctctagag gtaaagggtg tatccgataa gtcgggtgtc caaaaaaact taatatggtg
     2281 agtatgtaca aacgtactga cgaactagcg aatgtactgt ccgaaaggca tagctcctag
     2341 aaatgggaga gacagtgagc aaaaaatcac tcattgtgct aacttgtggt tgagtaaaag
     2401 tcgttgttat gaaagaccat gctgcgttac aggcgtagga gaagttaggg ggcaaaagct
     2461 agcatctaag ttaaggcatg tggatagata taagggaaca ggggaagcca tcaacatgga
     2521 gagtgcatac tcgatgaagt ggtagtaaga aatatgtgaa tgacaaataa cttactttag
     2581 tgatggtgaa agtagagtca cgactgatga aggttctgta atggaactgg aggaacaggc
     2641 tcgattcgtc tgtaaaagtc atgaaacccg ttcaaccatg ggattctcgt atgactaaaa
     2701 aggtcacgag ccaattggag cgtgataaac catgacaaaa gaaacaagtc caaaagatag
     2761 gaagttgcgg catgcagaat attatggcat gacggaaatc ttcgataaga tatactccaa
     2821 aagtttgaag ggagataatt tcaagaatct aatgcagatt atcatttctg ataacaacat
     2881 tgtcctagca tatcgtaata tcaagagaaa tcacggtagt gtgacaagag gagaagatgg
     2941 agtaacgatt aaagatattg aaaatctcaa ccgtaatgaa ttcgtagaaa ttgttaggaa
     3001 gcgattcgca cattataacc ctcggaaagt aaggcgtgta gaaattccta aatataatgg
     3061 taaattaaga ccgttgggta ttccatcaat atgggataga atcgcgcagc aatgcatctt
     3121 acaagtatta gaaccaatat gtgaagcaaa attcaataat cacagctatg gtttccgccc
     3181 aaatagatca gcagaacacg caatagcaga ttgtatggcc cgaatgaatc aaagtcacat
     3241 ggaatatgtg gtagatgtag atatcaaagg tttcttcgac gaagtcaatc atactaaact
     3301 catgagacaa atctggtcta tgggtatacg agacaaacag cttctcttaa tcattaggaa
     3361 aatgttaaga gcacccgtag tacttccaaa tgggaagttg atatacccaa caaaaggaac
     3421 accacaaggc ggaatactta gcccattact tgcgaacatc aatctcaacg aatttgactg
     3481 gtggatagca aatcagtggg aggaaaaaga tgccaaagag ctaaaacatc attttaggaa
     3541 aaatggagct tgggataaag gtaatatcta tcacaagctc agaaagagta caactctaaa
     3601 agaaatatac ctagtgagat acgcagatga ttttaaaatt ttctgtagaa attataatga
     3661 agcgaaaaaa gttttctatg caactaaaat gtggttacag gagcgtctaa agttaccaat
     3721 atctgaagaa aagtcaaaaa tcacaaatct ttcaaaagaa gaaagtgaat ttttaggctt
     3781 tacgatgaaa ttagtgacca aaggtaacaa aaaggttgtt aatacgcata taggtacaaa
     3841 agcacaaagc atgattaaag tgaaactaaa aaaacaaatc aaacgaattc aaaaaagtcc
     3901 taatagcaaa gataccttta tctatattgg agtctataac agtatggtta ttgggattca
     3961 taattacttc aaaattgcga ctcattgtag tgtagcaatg cgaaaaattg caagagaact
     4021 cagaccaagt tttgaaatac gactacgaga acaaggaatc tcaaaagaag gacaatacgg
     4081 aaagtacgaa ggatacaagc cttacctacg gtcaggacaa atacgatata taagagggtt
     4141 tccaatatta ccagtagggt atattcaaca cagacatccg atgtgtaaaa aaagaaatat
     4201 taataaatac accgaagaag gtagggaagc cattcataag aagcaacaaa ccgtagaaga
     4261 atggaagata tcgtggttgt gtagcaatcc agtgcttagt aagagagcaa cgattgaatt
     4321 tgcggacaat cgaatttcaa aattcatcgc tcaaaaggga agatgtgcag taacaggtga
     4381 ggagcttatc ttatctgaaa tgcattgtca tcatattatc ccttatcatg aaagcaaaag
     4441 tgatagctat gaaaacctag tgattgttac tgaggaggtt catcgggtaa tacacgctac
     4501 acaaagtgaa acgattgagg aacttctaaa atatctaaaa ttaaatccaa aacaaaaaga
     4561 aaaactcaat gagttgagat taaaagttgg taacgaagaa atatcttaat aaatatttgt
     4621 aggacgggaa aacagaatgt cttttataga cgagggaacg ccgtatgcgg tgaaagtcgc
     4681 acgtacggtg tggactgggg gaaaagacgg agataatatc aaactcttac ctatcagtat
     4741 cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa cgagaaatgc
     4801 gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa
     4861 atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga
     4921 tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtg ggtaaatcaa
     4981 tgacgaactc agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaatt
     5041 ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt
     5101 ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaagcagt atgttaagcg
     5161 tcgtgcaaag cggcgcttat ttttgtggtt gtttggtact tctgcaggtt tgattactat
     5221 tttgatcact gtgttcgtga ccctatttct aatacttgcg gcgggctcga ttgataattc
     5281 ggattcagat tcaagcagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc
     5341 gatttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgccgt
     5401 aggtgcagca gtgaaatata aattattacc aagtgtgatc ctttcgcaat acggttacga
     5461 atcagcgttt ggtacatcgg cgtccgcaag aaatgatctc aactattttg ggattacttg
     5521 gtttgatggt tgtctgtttc ctaaaggaac tgcacgagga attggtggca ttgaaggtgg
     5581 ctggtacatg aagtttccca actcaaaagc tgcattttcc tattacggct ttatggtagc
     5641 cacacaatcc aattttaatg cttgtgtagg aaataaaagc cctggcgcaa gcttattgat
     5701 cttaggacga ggcggttatg ccgctgcagg aattacggaa gatagtccgt attatacagg
     5761 ttgtatgtca attatcactt ctaataaatt aaccgaatat gatgaattcg ccatcaaaca
     5821 ttggggtgaa ggtggcaata acaacggcac gattactggt gaatggacga atccattccc
     5881 tggcagcagt ttagataaaa gttccttcag cggtggtcaa ttatttggaa cgaatccagg
     5941 cggtgaattc cgtccgaacg gcttccatga tggattagat tttggttcag ttgatcatcc
     6001 aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg tacgttggca atcctggtat
     6061 ttcaggtttg ggtgcgtgtg tgatcgtgat taattacgat ggcttgaata tggtgtatca
     6121 agaatttgca aatagtacag gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg
     6181 tcaagtaatt gggatacgag atacagcaca cttgcattta ggatttactc gaatggattg
     6241 gcgtcaagca caaggacatg cattcattga tgatggtaca tggatcgatc cgttaccatt
     6301 tttgaatagc agtaaaaaat agatcaggag aaaaaatatg gaaaataaaa cgttaaaaat
     6361 cgtcttaaca gctgcaatta gcgttagttt aatcggcaat attgcgttgt tttggcagct
     6421 gaaagaggaa caagcagcaa atacctataa aattgaagaa gtggctaaaa acgcgtctga
     6481 gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa atcaaaccat
     6541 caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga
     6601 ggaagtaact gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa
     6661 atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat
     6721 cgacaaatta agtcctacag acgatgatca ccaacatgat gattacggaa gttactcctt
     6781 tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg aacaaaattt
     6841 tgtcgtattt gttgattata caatcggcaa tccgcaattc aaagatgtca aaccacaaaa
     6901 aataaaaggt ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcataga
     6961 ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gaaagacaac
     7021 gtttcaaaaa acgaaaaagt tatcttagat ggctgggaat ctttatgatt ctcggctttt
     7081 tgattcttgc tagtccgatt tatacgaatc agaaggtaga tataccagat gaggtaagca
     7141 cgaacgcctt tttaaacacg aaagagctga caatggtttc aaaagaaaaa ggagcgaacc
     7201 agcaattagt ttttgaattt gcgatcgatg atgaagatca aagcttgtta aaagaactgg
     7261 ctaatcttaa ttatcgcgta gaagtaaaga cagctaaagg ggattatcaa gcaatacgag
     7321 ccaaagtgat taaggtgtct gatgattatt ttgcagtgaa gctaacgcat gtaccagaag
     7381 aatatatcgc catgcgttta accattattc ctgaaaaaat tgatccaaaa gtggatatgc
     7441 aagaaccaca agatctgatt ttttatattc acgaagataa ggtcaaagat cacgtgaaag
     7501 acgaagatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa
     7561 aagcagctca aaaacaaatg gaatctttcc aagcaaccat tgatttgaat gaagaattag
     7621 tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga tgatcaggaa aatactgaaa
     7681 acaagattaa tagctaccag caagaaattg aaacatcgaa aacgcaactg aaagatcaag
     7741 aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aagatttaga
     7801 aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc tgacaaagat actttcgctg
     7861 agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcgag
     7921 aagtgaattt atctaacaca acaatatctg agttgaagaa tggtcgaaag aatttagata
     7981 ccttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat tttacagctg
     8041 ataacaaata aaaaacagga gtattttatg tgaaaaaagt atataaaaaa tgcttatgaa
     8101 tcagctatat caccttaacc atttgttaat gagatgggag aaaacattaa ccacgattct
     8161 aacgcaaaca tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa
     8221 ccaaacagga agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat
     8281 atgacaaaat atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca
     8341 caaataacct taatttttaa agagcataag gaaacgtgat atcactttct cacgaaaggt
     8401 gttaggtagg ctgtgcctac cacgtttcta agaaagataa atgatatcaa aattttggat
     8461 ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg cagtaaaata agcgtttgtg
     8521 tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta cacatatccc tacacataat
     8581 aaatttggag gaaaatatgg acttattaat tagaaatatt gaagaaaagt atattaataa
     8641 aattgacaaa cgttgtgaag aacttacggt caagactggc aaaaaatgga gtcgaaatca
     8701 atacctaaaa gttttgatcg aaaatgattt tgatcacgct cttctgaatt ataaaaaaga
     8761 ccaatttgat cggttgttag aaaaatttgt ggatattcaa acttataata cgaaaacttt
     8821 agaagaatat attgcaacaa ataatcaatt aattggtttg ttaattgaat aataaaaata
     8881 gtattgtgaa tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta
     8941 tatctttacg atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata
     9001 tctaattata gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa
     9061 aaaataaatc caattcaaat taaattgaat gcaatgggaa aagaaatttc tattttaacc
     9121 gaaatggttt cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca
     9181 aattcaactg tttatcaaga agcagaagaa cgtgtggaag gaaaaattaa atccaatcaa
     9241 accaaaaaat attcttctaa gttaaagaaa ccagtgaatg aatctgttca taccagagta
     9301 gatccatttg atccaacgaa gattctaaaa agataaaagt agaaggagaa cgtgatgaat
     9361 aaatcgcctg cagttattct tacttgtcaa tttaccttgc ccaataaaaa atctttttca
     9421 acatacattg attatatgac aagagaaaaa gcactggaag aaatggaacg acgtacgccc
     9481 gaagaagaac aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa
     9541 ggagaaactt tttcggaaag taagacggat aaagaattca gtgaaacgtc gaatgaagct
     9601 caatcgttga tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaaa
     9661 tatctttctt atatgacccg tcactatgcg ttagaacaaa agaaagaact gacgcaaagg
     9721 gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa aatatcaaac agagccacat
     9781 aaaaaagctt caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga
     9841 agtgatctga aggagatcaa acaaatcttc caaagaggac aacaaaaagg ctcaatcatt
     9901 tatcaagatg ttatctctca cgacaattcg tatcttgaaa aaatgggctt gtacgatccc
     9961 aaaacagata ccttagatga agaaggtttg aaagcagctg gaagaaaaat gatggagaca
    10021 ttattcgaga aagaacaatt gaatgaaaca ggttactgga tggcaacgat tcaccgtaac
    10081 actaagcata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccgtat
    10141 actgagattg aaaatggtgt gagttatatc gtaccgaagg ggaagagaag acaagcaaca
    10201 ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac gtttctctta tgaaaaggat
    10261 cgaatgatcg gtatggagaa acgaaattta ttgacacgaa aaagtgattt gcgtaacacc
    10321 ttaacaaaag tagtaaaaga accagcacgt tacgatcgac acgccatgaa cttattaaat
    10381 gaagtatata aaaccctacc tgaaaaacgt tcggaatgga attatggaga tgaaaatcga
    10441 accaagcttt caccatttac aaggggaaaa cttgatgagt taaccaaata tgttttaaca
    10501 aaagaacctg gatatcaaga atatatacag atttcaaaag cattgaaaga agaagccaaa
    10561 agcctttatg gggaaagcaa aagagaaaca aagaatgcag aaaaaaacgc cctctttgat
    10621 ttaaaaaagc gcacaggaaa tgcgatttta acagaactga agagacagga tagtcaaata
    10681 aaaccattaa tagaaagtgt caatcagatg caatctattt acaatgaacg atcctttcga
    10741 acactcaaac agttgccaac aaatgaatac aagtatagtt atcaagaatt cgtaaagcgt
    10801 aggaaacaat tccaacaaag agttgtcacg cagcgaagct tgcgtcaatt aaatcgaaca
    10861 attaatgcac atgaagaaaa ataccacgca agtaaagcat atgaagagca acagcgaaga
    10921 atccaaatgg aacaagaaag acaataggag gaagagaaat gaaacaatat ttagatcaat
    10981 ggaaagtcat agaaggatca ctaaaagaag aacgaatcga acaacttcct gattgtttag
    11041 aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa cgaacagttt gatccaaatc
    11101 aatttttagt tgttgagtac ccagctactg gtgtgtattg ttgtaatcat gtgaagggtg
    11161 aaaaatattt tattattcaa gaatacgaag gtaagttagc gccttattat actacttggg
    11221 aaataaatga agagggaatc aataatttcc catgtgaaag cattgaagag tctatttctc
    11281 tcacggaatg ttaaataaat agagacgtaa aagagtcctg aacgtgattg tccaggactc
    11341 tttttcaatg aattgttttc cgccaattca ctgagtggtg cgattcaatt ttattatatc
    11401 aaaagaaagt gagattccaa agtgaaggga ggaagaagaa atgactcaga caacgattgg
    11461 tgttgtggtt gaaaaaagca gggataatct cgtttttgtg acagaaattc aaacaggtag
    11521 agcttttgta gtcacagaca aagctgcaaa agcgtatcaa aatggcaata ttttaacgtt
    11581 aaatctgact acgaagatgt tcgttgatgc agtagaaaat tatccatttg tttagacaaa
    11641 cgatatttta caaggaggaa acggacatgg cagaagaaaa agaaccacta gataatagtc
    11701 ggttgggaaa gtccaaaaga aaattagtac ggttgcaaaa cgaattaaat aatcaatttc
    11761 aaaagctatt tgatcaccaa aaattgacaa atgggcaacc aatgaatgac aaacgaaatg
    11821 gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa aatacaaagt ttgagagaag
    11881 agatcaagca acaagaaaaa caggtagaaa aacttgaaag acaagaagag ctaaaggaaa
    11941 tgggctataa caagtacggc ggactggata tgacgatcga taatattcca agaatcaaag
    12001 aagaaatcga acgctttgaa aaaggtgaat cgactttttc agccgccacg atccgaaaat
    12061 atcaacggaa gttggaaaca ttagaacagc tgaaagaaaa atcagaaaaa ggaaaagaaa
    12121 atatcctccc tgaagtacaa gcgatcattg acagcggacg agtgacgcaa tggaaaaaaa
    12181 atccaaccat ttattttttg aaaggctatc gaaaagttgc tttagaactg aatgaaaacg
    12241 ggggatttga ggaatcaaaa aaataccaag ccacgtcgga agaggaaaaa gcagctgttc
    12301 gtgaattact tgaagaggct gcaccaactg attctctgaa ggagaaagaa aaagatcatt
    12361 tctatcgagt agaattcaac gaaaatgatc cagaaaaagg attgaaaagt tatggtgggg
    12421 agatcgtaac attagaattg atcgatcaac ttaaaacgtt ggataaccaa tacgctgcag
    12481 aagcaggtta ttataaattc tattttgaag aagtgaagaa cggagaagta gtagatcaga
    12541 tccgaatgga tctaggggat ggcgtcaaca gtaatcaccc catctattta gagttagcaa
    12601 aattgtgcga acaagtacct gagaaggaac aaaaacaatc ggtcaaaaat gaacctgttt
    12661 cgaaagaaca aatgcagtcg aaagaaacaa aaacaaaaca aaagggctat caaaaacgaa
    12721 cggcagaaga gatcaaacaa gaagttaaat ccctttcgca acaggcactg gatgcggtga
    12781 aaaagcatac ccattcttct gaagacgtca aagaactttt gaactttatg tctcgttttc
    12841 cagaacgatc ctttagaaat caattattga ttgaggaaca atttccaggt gcaacggctt
    12901 gtttaggtcg tgccgcgttg aaaaaagaag gaatctatat caaaaaaggg gaacaaggga
    12961 acaagatctt tgtcagaaaa acggtcaaag gattttatga gaaaggacgt ggctttgttc
    13021 gagaaacaga agctacacca gaagataaaa aacgaatcga aagtggtcaa atcgaagtga
    13081 tcaaaaaacc ttactacacg attgaaaaag tgtttgatat cacacaaaca cagttaaaac
    13141 ctgaagacta tccgaaaatc tttccgaacc gtgtctttga ttttcaattg gataaaaaag
    13201 gacagtcaga acttcaagct gggattcaag cggtagccaa caatattgga atacaaattc
    13261 gagatatgac agaaagtgaa gtctatcaaa gagaattagg acaagcacgt ggggcgtatg
    13321 ttcgtaacga atttactggc gaagaagaaa tcgtcatgaa tactcgaaat actcctacgc
    13381 agcatttagc tacaagtatt catgaattag cccatgcaag aatgcacaaa ttttcagaat
    13441 tagataccgc aactaaagaa ttgcaggcag aaatgacttc ttatattgtg tgcaaacatt
    13501 ttggaatgga cacttcggag aaagctattc cttatattgc agcatggacg aagaatggtc
    13561 agacgttgga tgataaggaa gcggcggaac gtgggaaaat catgaatgat gtcagccgtg
    13621 tcgcaaatga attcattcaa accatttcta atgaaatcaa tcaatacaga gaaagagaac
    13681 cagagatcca accagcagaa gtgaagcagg agcataaaaa tactgtagaa acggcagaga
    13741 ataagaatat tgtgcccgtt ggttcacttt ggatcgacaa gaaagacggg aaagtaatgg
    13801 aagaagatac gggtcgaacg ttacatttga aggatgcggc gtttattgaa gatccgcagc
    13861 ataatcaagt gattttaaaa ggaaaaagtt atggaaaaga gattcaaacg gcgttacctt
    13921 cagaagaatt tgcgctagga aatccattcc gattttcact tgaaacagtg tcagaagtgg
    13981 ccaaacgaaa aaatgaaatc tattatgaac aacaattgtc acaaaaagaa cgcgtataaa
    14041 acaagtaaaa aagactatct gaaaaacgga tagtcttttt tcttttggga ggtttgaaaa
    14101 tgaccgaaaa aaaaatgagt cttattgatc gatgtaaaca gatcgatata gtggattttg
    14161 ctcgaaacaa tggaatggct gtagtcaata aaggacgaga ctatcgctta gaagaccatg
    14221 attcatttgt ttttgatcga agaaaacaac gtttttattg gaacagtcaa aatattagtg
    14281 gcgacattat tgaattagct aaactttttt tcatagacaa agagatccaa gactctaagc
    14341 aacaatttaa agcagccact gattttattc taaagaatga agacaaaaca gaacgagtgg
    14401 aaaatcttca ttttgaaaca gaaaagtaca aagatcaccc cgttgattat caaccactca
    14461 cagaaaaagg aaggaattat ctgaaggaag aacgaaaact accagagtgg ctgattgatt
    14521 atgcagaaaa agaagggttg attgccgaac taaagccaaa atatgaacgg caaaatttct
    14581 tagttcgaga cgatcgttta gatcatgcgg tcgccttttt atggaaagat ccacagacaa
    14641 aagagacagt aggggcgagt tatcaaggaa cgttcattga ttatgaacgt tttggtgaga
    14701 gaggcacata taagcatata gataaaaatt cgacagcgaa tcatggcttt aacctaaaga
    14761 tcggtgaccc taagcagtta aaattttttg agagtagtat tgatttactt agctatgctg
    14821 cactgaatcg tgatcagtta aacgatacgt ggttagtctc tatggaaggg ttaaaacacc
    14881 atgtgatcag tcattatttt ggtgaggcag tatctgaatt gagaaagaaa caagcctttc
    14941 ctcaatccat cgagatttgt gtggataatg accgtgcagg acatattttc tacgaaaaag
    15001 aacaattaat gggcgcagtc gatccattta cgaatcagaa agtacgttgt gaacgaggaa
    15061 tcgctaatga ttggcaagtg ccaaaagaat acaaaatcat ttatgaagaa gtcgcaaaag
    15121 aagaaaaggt cacaccagaa gctattatgg ccatacacaa gacagaaaac aatctgcaat
    15181 taacgaacca actggtttcc gctcacaaag taaatgcgtt ttttggccag cagctttctg
    15241 ttaacgattc aatagaagcc attaatttga aagatatttg tagagaagtc gcaaaagaat
    15301 taaaagtttg tgaacgcgtg gatggtacgt acgattttga tcgtttttat caagaaaaag
    15361 gggatattaa tgcgcagatt cttttctctt ataaagcaga acaatactac aaaggctata
    15421 aaaatcatga acatgaattt atacctgaag taaaaaagga ctggaacgat caattgaagc
    15481 atgagatcca gcaacaagaa atcagaaagc aaaaacgtgc catgttgttt caacaaggca
    15541 gacaacaaga aagggagtga aatcgtcatg atgaaaaccg ttattttagc agaaaagccg
    15601 tcacaagcga aagcttatgc cgatagcttt tctaaagcta cccgaaaaga tgggtatttt
    15661 gagatccaag atcgattgtt tacaggagaa actgtgatca cgtatggttt tggtcattta
    15721 gtcgagttag attcgcccga tatgtacgat gaaaaatgga aacaatgggc attagaacat
    15781 ttgcctattt tccccaatca ataccactat catgttccta aagataagaa aaagcagttt
    15841 agtgtcgtca aacgacagct gcagtcagct gatacgattg tgatcgcgac tgatagcgat
    15901 cgtgaagggg agctgattgc ctggtcaatc attcaacaag caggcgccaa tcaaggaaaa
    15961 acctttaaaa gactttggat caactcctta gaaaaagaag ccatttatca gggattccaa
    16021 cagttacgag acgcagaaga gacctatcca aaatttgaag aagtacaagc acgtcagatc
    16081 gccgattggt taattgggat gaacggtagc ccactgtata gtttactctt gcagcaaaaa
    16141 ggcataccag gaagtttttc tttaggtaga gtccaaaccc caacactcta tatgatttat
    16201 caattgcagg aaaagattag aaacttccaa aaagaaccat attttgaagg aaaagcccaa
    16261 gtaatcgctc aaaatggcgc atttgatgcg aagcttgatc caaatgaaac ccaagcgaca
    16321 caagaagcat ttgaagccta tttaaaagaa aaaggtgttc aattaggaaa acagccaggt
    16381 acgatccttc aagtcgaaac agagaaaaaa agtgcggcta gcccacgtct tttttcttta
    16441 tcgagccttc agtctaaaat gaatcaactc atgaaagcta gtgccaaaga tactttggaa
    16501 gccatgcaag ggttgtatga aggaaaatat ctaagctatc cgcgaacgga taccccttat
    16561 attacggaag gggaatatgc gtatctgttg gatcacttag acgaatacaa gcatttttta
    16621 aaagccgaag cgattccgac tcctattcat acaccgaata gtcggtatgt gaataataag
    16681 aaagtccagg aacattacgc gatcattcct acaaaaacgg ttatgacagc agcggctttt
    16741 gaacaactaa gtccactgca gcaggctatt tatgaacaag tgttaaaaac aacggtggcc
    16801 atgtttgcgg aaaagtatac ctatgaagaa acaacgatct tgacgcaagt tcagcagctt
    16861 cagttaaagg caatcgaaaa ggtgccgcta gatctaggtt ggaagaagtt atttggaaaa
    16921 gagagcgacg gaaaagagaa ggaagaagag ccgctacttc ctaaagtgac aaaaggagaa
    16981 atggtgacgg tggatctaca agttcttgaa aaagaaacca agccaccaca gccttatacc
    17041 gaaggtacgt tgatcacggc gatgaaaaca gctgggaaaa ccgttgatag tgaagaagcg
    17101 caatccatat tgaaagaagt ggaaggaatc ggtacagaag cgacacgggc gaatattatt
    17161 gaaaccttga aacaaaagga atatatcaag gtagagaaaa ataagctcgt ggtgacgaat
    17221 aaagggattt tgctttgcca agccgtggaa aaagagccgc tgcttacgag tgccgaaatg
    17281 accgccaaat gggaaagcta cttgttaaag atcggtgaac gaaaaggaac acaaaccact
    17341 tttctagcca atatccaaaa atttgtctct cacttattgg aagtcgtacc agggcaaatc
    17401 caatccactg attttggatc gacgttacaa gaagtgaaag cagccagtga aaagcaagaa
    17461 gccgcgcgtc atttaggaat ttgtccaaaa tgccaggaac aagaagtctt actctatcaa
    17521 aaggtggcag catgtacgtc tgaagcctgt gatttcaaac tatggacaac gattgccaaa
    17581 aagaaactta cagcgacaca gctgaaagag atcattcaaa atagccgaac aagccaacca
    17641 gtgaaagggt taaaaggtca gaagggttcg tttgaagcaa cgattgtttt aaaagaagac
    17701 ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa aagaacacga
    17761 cgcactacaa aataatggag ggttaatctt atgcgtaata caacacacaa acaacaagaa
    17821 atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc cagcaaacga
    17881 agactgcagc aattgaagaa ccagcagcgg attcaaaaag aaatcgaatt agatcgataa
    17941 attcctttct tgttaatata aaactaacat gttatagttg tgttagtttt atattaactt
    18001 taggggaaga gtaatgaaag acaaaatcca atgtattttt gataaacata atggcaacct
    18061 atccttgaag gatgctagaa gtgaaggatt atcgcctatg acgttagatc gattagccga
    18121 aaaggataaa atagaaaagg tagcaccagg tttctatacc ttaaaggatg cgttcgtgga
    18181 tgaattgtat ctcgctcaga gtatctattc cagagggatt ttttctcatg agactgcgct
    18241 cgatcttttt caagtaggaa catatatccc taaaaagatc aatatgatgt ttcctaaagg
    18301 atacaatgta tcaaaggaga aattagatca gtatgccatt caaccgcatt acactaataa
    18361 agaaaatttt gacctgggag tcacagaaac agaaagcttt tacggcaata aaattttagt
    18421 ctatgattta gagcgaacgt tatgtgacat gtggaatcca agatacaaag caagcgtaga
    18481 ggtaaaacaa gaagcattaa aagaatatat gacaagtacc agtagaaata caaataagct
    18541 aagaagatac atggatattt taaaatcacc aaaagaaatg actttgtata tgttgccact
    18601 ttattgagag gagtaaagat gaatgacacc gacacaattt aaagagaaaa caagaaattt
    18661 cgcagcagag aaacatttaa acccacaaat tgtccaaaga tattatatga tggaaaaatt
    18721 attagaatgt attgttgaat cgccttatca tgatgatttt gtattaaaag ggggatttct
    18781 gattggatca aaatacgggt tagaaaaccg ttcaaccgtt gatattgata cgacttatcg
    18841 aaattcgaaa ctaacaaaag aaaagttaga aactgttttt tctgaattaa cagctaatcc
    18901 tacaaaagat ggaattcgtt ttgaattaaa gggattaaca gagacgaggg aagccgatta
    18961 ttatccaggt tttcaagcaa agtttattgc ttatcttgaa aatgcacgtg taccttttaa
    19021 attagatatt acttcaggtg attctattat tccagcagca ttgatgtatg aacacaaatt
    19081 aatgtttgaa gataaaacga ttcatattcc agcttatcct acggaacaaa ttctagctga
    19141 aaaattacat gccacgttta gttttggaaa agataattcg cgcatgaaag atttttatga
    19201 cctgtatatg attccgaaat tagaacgtat cgatgagaaa aatttgtata agagtgttca
    19261 aggaacattc aaggaaagaa ataatcaatc ggtgtttaag catttgtatg atcaggaaat
    19321 gcctcaattg gtgaaagatg agggcttgag agaaaagtgg aaaagatacc aacaagaaaa
    19381 ctattttgcc aaagacattt ctttcgaatc tacactaact tccgttgatt ccctgatgca
    19441 atcaattgta gagcaagaga aggcagaacg aaaacaaaat tttaagagat cacaacaaat
    19501 gaatcaagaa atggagagat aaagatgatc aataatgtaa ctttagtagg gcgattaact
    19561 aaagatccag atttacgtta taccgcaagt ggcacagcag tggcgacttt ttccctagcc
    19621 gttaatcgta atttcacgaa tcaaaacggc aatcgagaag ctgattttat caattgcgtg
    19681 atttggcgca aaccagcaga aacgatggcg acattagcta aaaaaggcag cttgatcggc
    19741 gttgtcggac gaattcaaac acggacttat gacaaccagc aaggacaacg tgtgtatgtg
    19801 acagaagttg tggccgataa cttccaacta ttagaaagta aagcagcgac agaaagccgt
    19861 atgcacgctg atcaatcgtc cagcagtcca agccctacca cctttgaaca acgggataca
    19921 gcgacgccta acaacaatgg attgaacgct tcacagaatc catttggtgg acaatcaatc
    19981 gatattagcg atgatgatct accgttctaa gtagcatttt ctattggttg catttgctac
    20041 ggtttatgtt ataatttaga caacaaaaag aattttcaat ctataaccaa aaaccactag
    20101 ctggcgggct agtggctttc ttttacgttt ctattataag aattaacggg taggcttact
    20161 gtctttcttg gcaaatttac aatcaagcca tttgcagata tagtgcgcgg ctacttctgc
    20221 catgattgca aaaacaaaat caaaaagaat tttcaaacct ataacctcct ccccttgcca
    20281 gattaggccg gggtagcaga cagcaagact attatagcat aattgaaata tccaaatgat
    20341 tggatttttg ctataatttt agcgaggtga ttgtgatgaa caaatatgtt ggtttgcttg
    20401 ataaaatccg agtaataaaa acgcaaccgt tattggttcg ctttacttta caaacgattc
    20461 atgaatcgat taattgtgta gttgcagata ttgaaattat cgataaattg ctaattatgg
    20521 atgatggaaa atacaatatt gccgtcacag gtcacttcaa taaacgaaat cagctagtta
    20581 ttgctagcat gtatgttaga aatcccgatc attttacgcg atcgatgggc atataaataa
    20641 aagaagagtt gtagtcctaa atgaggattg cagctctttt tgttttacat agaaacagga
    20701 aaagaccttt ttaggaagac acaagtgcct tctcaattga aaaacctttc tctcactcca
    20761 accgccaccg ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg
    20821 tcttgcaaag caagacaaaa tagacaaaac ttcctctcac caaaaggttc gttcctgttc
    20881 gttatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga
    20941 caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattta
    21001 ttctggccaa caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga
    21061 ggaggcagaa tcaaacatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt
    21121 attcccaaac tatcatgcac cacatactgt tgaatgcgat tgtggagaaa gagcgaaatg
    21181 tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta
    21241 tggcgattac attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa
    21301 ttattgactg taaaagaagt cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa
    21361 gtagcagaag ctttacttat tccgcggtac tggacgatag aaaccccttt agtagaggat
    21421 gcaccgacta tgctatttga agacctagtg aagattgcag gatatgaact gcattatcaa
    21481 gaaaacatgg aaacttatga aatcgaatat tggttaacca aattagagaa ctaaaaagga
    21541 gaaaaaatat ggctcaaaat tactatgacg aatttgtcaa attacctttg gataaaatgg
    21601 cacaaaaaat ggaggatatg acgttcctat accatgaaac acgtgtaccc aaaaaacatt
    21661 acaaggaaaa attgtcagtc gcagtggaag aaatgattga atcaggtgtc gaaatgaatc
    21721 tcattgcgac gtactatcga acactggaag aactgaaaaa acaaaatgga aaatggtttt
    21781 tccaagcctt actatgcttg gaagtaggtg tcaaaccaag cactatcaaa ccttccgaat
    21841 atcaagcctt agaactgact tatgcaaaat tcgcacagat taagaaagct aaagcaatgg
    21901 ctagcgactg gctagatact tttgaaagta tcgaaaaaaa tggtgcattt tatacgattg
    21961 aatcggagga gaaagataat gaataaggtg gtttatcagc taacaacaga agatgtttcg
    22021 caatttttaa caagcaaaga acacttgaat gaagtaatca gacggattca aaaagacttt
    22081 agttgtatgg aagaaatgaa gtactttatt caaagagtgg aagaacagtt gtatatggaa
    22141 agcgaaacaa gtcaaacgaa ggtcacagtc agccataatc atttagacgt gtataaacgt
    22201 cgtcctgaaa cggtaaaaaa aggaacagag cttctacgac tagaaacaga aaaatacatc
    22261 atgaatgtgt acctagaaag gagtgagctg gtcgaaaact ttgtcagcta cacgatttgt
    22321 aggaaacact attactcaga atcagaatat tattcaattt tcgagagcat ggaattaatg
    22381 gaagaagaaa tggaagagct aaacagcaaa aatgcgacgg tagacgatta ttaccttgta
    22441 tttactaggt ttttgacgtg tgggacggaa tctatcgtgt taatgggaat ggattatgaa
    22501 ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag aagtagataa cccaggcgac
    22561 gactggtaca cgactttaac tgatttttta gacacctaca cttacgacga tacagaagaa
    22621 atccaaaatg aacttgaccg taaacgaatg ccatatcaac gatttgaaag gaaaaaaggt
    22681 aaataaccat ttataccgaa aagtgaggat gtgtgaaata agaacaaaaa accttgaaat
    22741 taagggaatg gttcttattt cgcaccaatc gctccaaccg cccccgccgc ttaggtggtg
    22801 cgaaataagc gggcaaagcc cgataaccta agagaaagaa ggagatccat atgaataaaa
    22861 tcattcctct attgttcgta ctgtttgttg gcgagatact tatgcgtcat gctgcagctg
    22921 tacatttaaa cttcgtagta ttcttaggaa tactggtatt ttgtttgatc ttttggcgat
    22981 ttgtattagc ccttgtgagt ctactattca agatgattgc agccatattt ttcatagggt
    23041 tatttcgatg aaaggagaga gtactaatgc caattgcaat aggaaataaa cggttacccg
    23101 tgacattaga tgaaaagaga caaaaagaat tgcagcaact aaagcagaag tacggaaaaa
    23161 gtgaatctaa gattatgtgt attgcgttag atctattgat cgcgcaagaa aaagcaggat
    23221 ttgatatacc agcactaaaa aagtgacgtc acttttcatt tgaagaaaca aaagtgatag
    23281 cacttaattg ttcctactat ttagaatatt ttctatgtcc tttttattcc attttgtatc
    23341 aataactatg aaatctttaa agtatccaga agagattatt tttagatcaa tttttttctt
    23401 acctgattct aaagattttg ttttcttaaa catattatga tttcctttcg actattttca
    23461 tataaatgtt aacatagttt atggattcat ggaatgttaa tctaatttaa tagaaaagga
    23521 taattaagta tatacgtttt ttcttatgta tgttatacta tgtatataca gaaagaggtg
    23581 tctactatgg aaatgttacc tataaaaaaa tggggaaata gtaatggcct tagattgcca
    23641 aagtatatta tggaatatct tggaattcat acagaagaca aagtgaagat aatacaagaa
    23701 gaagtaaacg gtcagaagcg gctaattatc gaagcggcta atatagaaaa tgaattaaca
    23761 attgaacagt tatttgaaaa ttacactgat gaaagaaatc atgtgacgat tcaaaattta
    23821 ggtgaagcag taggtaatga aaaatggtga gaaaacctag acaaggcgat attctattat
    23881 tgaatacagc acctcgatct ggacacgaac agacaggtaa aagaccttat attgttttaa
    23941 gtcatgatat tattgcggat tatagtaacg ttgttacagt tgctccaatt tctagtacaa
    24001 ctagaaacta tccgctatat gtaaatataa actcagaata tgaaatgaag acaacaggaa
    24061 aagtattatt agatcaacta acgacaattg attatgaagc cagagagtgt atttttttgg
    24121 aaaaagcgca tgatagttta gttgaggagt tattgatgaa agttagaaca gtttttcaaa
    24181 aagtaagcaa ataaaataga aacagcagca tgatcaacta ttcgtgattg accatgctgt
    24241 ctttttttat tctatatacg gtttgtctcg taacattgta gtcttttgca atcttagcga
    24301 tcgcaacacc tttttttaga tcctcaacga tgtttttata aacaagacga cgttggggat
    24361 cttttgcatt ggcactgtat agcttaggac gtcctttata aacaccgttc gcttttgcaa
    24421 tcttaatgcc ttgtgcctga cgtcgtttag attcggtacg ttcttgttca gcgatcatgg
    24481 ccaaaatttg aatgatcaaa tctttaatga atttatccag gagaggattg ccaattgctt
    24541 cggccatgat cggtagactt gtgatgatca gctgcacatt tttcttcttc aagtaattga
    24601 ctgaagcgat gatctcgtcg taatttcttc caagtcgatc aatagcttcc acaataaaaa
    24661 tatcttgatc tcggacgaaa tccagtgctt cttgaaaaac aagtcgatga gtaatcgttg
    24721 cgccagattg tttctcaatg aaaattctct ctgcaccgaa ttttttcatt tcttcaatct
    24781 gccgttgttc attttgatcg atggatgaga cacgtatgta tgcaactctc aaaattttta
    24841 cctactttct gcaactattt aaaaaacacc ctaatcatac acgagaaaac cagatttttc
    24901 aatgtcgcgg tcgtgtctag ttagggtata ccttatatag acaaaaacta atgatggagg
    24961 aaatagtatg tgtcactttt ctttttaaat ggcaatattt tttgtaaata aatgtcaatc
    25021 aagaaaattt aaacgattat gacattaaaa atatatttta tgaattttct agtatataac
    25081 ttgatttatc tgttaagatt tattaagaaa acgttttctt atttttgatt tcgaggtgat
    25141 agacaatgaa aaataagttt attttatctt taggtatttt aactattgga attagtgtaa
    25201 ctggaactac aattcctaca tttgctgata gtacaaatga accaactact gtagtagata
    25261 ataaccaaga attaactcct ttagatagga acggaatagc cattggtaac ccaactaacg
    25321 acttaactgt tactgaaaat tatttaatag tttctccatt acttgctcga gcagcttcta
    25381 aaacgatacg agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc
    25441 ctcaatcacg gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt
    25501 tacaaagtta taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta
    25561 gaaagtaggg attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg
    25621 atattctaag taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt
    25681 tacaggagac acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa
    25741 gaaaaaaaag agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg
    25801 tctagtcgac attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca
    25861 ctttcttttt ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa
    25921 caatatctat gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag
    25981 gagtgatttt tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt
    26041 tacaagaaga cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg
    26101 gggcaaaaag taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata
    26161 cacttgtcgt ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg
    26221 tcaatcgact gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg
    26281 acaaatcaag ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg
    26341 agcgtaattt gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac
    26401 ggtttggtgg tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag
    26461 tggatagcgg tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca
    26521 tttatcgtta tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt
    26581 tgtcatttaa tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat
    26641 tttttgtaag tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt
    26701 taatatta
//
LOCUS       DASJON010000014        26407 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJON010000014 DASJON010000000
VERSION     DASJON010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.014"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..111
                     /locus_tag="U3989_000463"
     CDS             <1..111
                     /locus_tag="U3989_000463"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN1713933.1"
                     /translation="QDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            375..1799
                     /locus_tag="U3989_000464"
     CDS             375..1799
                     /locus_tag="U3989_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1713934.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            2260..3369
                     /gene="pdhA"
                     /locus_tag="U3989_000465"
     CDS             2260..3369
                     /gene="pdhA"
                     /locus_tag="U3989_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN1713935.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            3372..4349
                     /locus_tag="U3989_000466"
     CDS             3372..4349
                     /locus_tag="U3989_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN1713936.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            4374..6017
                     /locus_tag="U3989_000467"
     CDS             4374..6017
                     /locus_tag="U3989_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN1713937.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            6025..7431
                     /gene="lpdA"
                     /locus_tag="U3989_000468"
     CDS             6025..7431
                     /gene="lpdA"
                     /locus_tag="U3989_000468"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN1713938.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            7593..7868
                     /locus_tag="U3989_000469"
     CDS             7593..7868
                     /locus_tag="U3989_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN1713939.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            7874..8641
                     /locus_tag="U3989_000470"
     CDS             7874..8641
                     /locus_tag="U3989_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN1713940.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            8809..10638
                     /gene="typA"
                     /locus_tag="U3989_000471"
     CDS             8809..10638
                     /gene="typA"
                     /locus_tag="U3989_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN1713941.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            10899..12671
                     /gene="recQ"
                     /locus_tag="U3989_000472"
     CDS             10899..12671
                     /gene="recQ"
                     /locus_tag="U3989_000472"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN1713942.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            12785..13087
                     /locus_tag="U3989_000473"
     CDS             12785..13087
                     /locus_tag="U3989_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN1713943.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            13090..14253
                     /locus_tag="U3989_000474"
     CDS             13090..14253
                     /locus_tag="U3989_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1713944.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            14279..17707
                     /locus_tag="U3989_000475"
     CDS             14279..17707
                     /locus_tag="U3989_000475"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN1713945.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            17728..18852
                     /locus_tag="U3989_000476"
     CDS             17728..18852
                     /locus_tag="U3989_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1713946.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            18842..19279
                     /locus_tag="U3989_000477"
     CDS             18842..19279
                     /locus_tag="U3989_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1713947.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            19263..19601
                     /locus_tag="U3989_000478"
     CDS             19263..19601
                     /locus_tag="U3989_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN1713948.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            19720..20280
                     /gene="rsmD"
                     /locus_tag="U3989_000479"
     CDS             19720..20280
                     /gene="rsmD"
                     /locus_tag="U3989_000479"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN1713949.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            20277..20768
                     /gene="coaD"
                     /locus_tag="U3989_000480"
     CDS             20277..20768
                     /gene="coaD"
                     /locus_tag="U3989_000480"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN1713950.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            20758..21801
                     /locus_tag="U3989_000481"
     CDS             20758..21801
                     /locus_tag="U3989_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN1713951.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            21960..22688
                     /locus_tag="U3989_000482"
     CDS             21960..22688
                     /locus_tag="U3989_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN1713952.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            22804..23049
                     /locus_tag="U3989_000483"
     CDS             22804..23049
                     /locus_tag="U3989_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1713953.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            23046..23264
                     /locus_tag="U3989_000484"
     CDS             23046..23264
                     /locus_tag="U3989_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN1713954.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            23400..23939
                     /locus_tag="U3989_000485"
     CDS             23400..23939
                     /locus_tag="U3989_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN1713955.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            23932..26205
                     /locus_tag="U3989_000486"
     CDS             23932..26205
                     /locus_tag="U3989_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN1713956.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            26243..>26407
                     /locus_tag="U3989_000487"
     CDS             26243..>26407
                     /locus_tag="U3989_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN1713957.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFL"
BASE COUNT         8866 a         4545 c         5737 g         7259 t
ORIGIN      
        1 caagaccttc aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa
       61 gttgactatg tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat
      121 aaaatattag gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa
      181 aaatagttct ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa
      241 catcattatt cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc
      301 aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata
      361 gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa
      421 ttatggaaca gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg
      481 aatcagaacg aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat
      541 tggaagagct tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta
      601 atcagactga atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc
      661 ctcgttatga gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa
      721 aaacatcgtt tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg
      781 agtttccttc ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag
      841 gctatactcc gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat
      901 tttcccccaa ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac
      961 aaacgattct ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat
     1021 tacctctttt caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga
     1081 tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca
     1141 acccgaattt tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag
     1201 tagaattgtg tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt
     1261 catttctaaa ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat
     1321 acgtcggttc gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac
     1381 ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata
     1441 tccttacaca aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag
     1501 atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat
     1561 cgtttttcaa gcttcattca attttaggtg gttactatgc ctggattact tgggatggga
     1621 aaaaactaga ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa
     1681 gtctactttt tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa
     1741 accaaatacc gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat
     1801 ttcacaaagt aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct
     1861 tttttttcgg aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc
     1921 cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt
     1981 taaatagaaa cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat
     2041 aaaacgcttt ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa
     2101 tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat
     2161 ttattaatga agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg
     2221 aagagatttt cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg
     2281 atcgacttcc aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt
     2341 gatcaagatg gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc
     2401 gttgaattaa tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg
     2461 aaccgccaag gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg
     2521 gctagtgctt acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct
     2581 caactagtaa aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct
     2641 gcaggtaatt cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca
     2701 caatatgtac aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc
     2761 gtcttcactt atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat
     2821 ttcgctggcg cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc
     2881 tctacacctc gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct
     2941 gggattcctg gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa
     3001 atggcacggg aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat
     3061 cgttatggac cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca
     3121 gatgacgatt ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa
     3181 ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa
     3241 gcagctattg ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac
     3301 atgttcgaag tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag
     3361 tcgaaataat catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg
     3421 aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag
     3481 ttttccgggc aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc
     3541 ctttagctga atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc
     3601 cagttcctga aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc
     3661 aaatggctcg tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt
     3721 cgccatttgg tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga
     3781 tcgctcagtc tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac
     3841 tattgatttc ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat
     3901 atcgctcatt ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag
     3961 cagttactcg tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag
     4021 caatcaaagc agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc
     4081 gtacagttgc gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg
     4141 tagttgttgt ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga
     4201 tctcagaacg cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata
     4261 cagtcttccc atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag
     4321 acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt
     4381 atcaatttaa attgccggat atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt
     4441 tcgtcaaacc aggagatacg atcaatgaag atgatacatt actagaagtt caaaatgaca
     4501 aatcagtaga agaaatcccg tcacctgtta caggaacggt taaaaatgtg attgttccag
     4561 aaggtaccgt agcaaatgtt ggggatgtat tagtagaaat cgatgcacca ggacacgaag
     4621 acaatgaagg ggacagtggt gtagccgcag aatcccaaac tccagctaaa ccggctgctg
     4681 aacccactgt tgatacagaa tcagctggat cgtcaagcga aggagtcttc caatttaagt
     4741 taccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag
     4801 gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa tcagtagaag
     4861 aaattccgtc acctgtcaca gggacagtta aaaacgtgat tgttccagaa ggcactgtag
     4921 caaatgttgg ggatgtatta gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa
     4981 ctagtgcgcc atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg
     5041 tcgttgaagc tgccgatcca aataaacgtg tattggctat gccatctgtt cgtcaatttg
     5101 cccgtgaaaa agatgtagat atctcacaag taactgcaac tggaaaaggt ggcagagtaa
     5161 caaaagagga catcgaaaac ttcttggcag gtggtccaag cagtgcacca gcgaaatcag
     5221 aagcgccaga agcggcagca ccgaaagaag cagctcctgc agcagaaagc aaaccagctg
     5281 caccagcaaa accattcaag tccaacttag gagacttgga agaacgtgta gcaatgacgc
     5341 caacacgtaa agctatcgct aaagcaatgg tcaacagtaa gcatacagct ccacacgtga
     5401 cattgcatga tgaagtagaa gtgtctaaac tatgggataa tcggaaacga tttaaagaag
     5461 ttgctgctgc aaatggtacg aaacttactt tcttgccata tgtcgtgaaa gctttaacag
     5521 cgactgttaa gaaataccca gtattgaatg catctatcga cgatgcaaac caagaaatcg
     5581 tttataaaca ttactacaat atcggtattg caactgatac ggatcacgga ctatatgtac
     5641 caaatgtgaa agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga
     5701 aagcaaaatt agctcatgac ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta
     5761 cgatcagcaa tatcggatct gtaggtggcg gttggtttac accagtcatc aactatccag
     5821 aagtagctat tttaggtgta ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg
     5881 aaatcgttgt cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt atcgtagatg
     5941 gagcaactgc gcaacaagca atgaataata tcaaacgtct tttagctgat ccagagctat
     6001 taatgatgga aggatgataa acaaatggta gttggagatt ttgctgtaga attagataca
     6061 gttgtaattg gtgcgggacc tggtggctat gtagctgcca tccgcgctgc tgaaatggga
     6121 caaaaagtag caatcattga aagagagtat attggcggtg tttgtttgaa tgtcggctgt
     6181 attccttcaa aagcactgat agctgcagga caccattatc aagagtcact agattctgaa
     6241 atgtttggag taactagtga aaacgtcaaa ctagattttg caaaaacaca agaatggaaa
     6301 gaaaacaaag ttgtccatac gttgacttct ggtgttggtt tcctattaaa aaaacataag
     6361 gttgaaacaa tcgaaggaga agcgttcttc gtagatgatc acacactacg cgtcattcat
     6421 ccagattcag ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca
     6481 atcgagattc caggatttaa attcggagga cgtgtgttag attctactgg cggattagca
     6541 ttgaaagaag tacctaagaa atttgtgatc atcggtggcg gtgtgattgg tgccgaattg
     6601 ggcggagcgt acgcaaactt aggagcagaa gtaacgattc ttgaaggctc accacaaatt
     6661 ttaccaactt acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc
     6721 gtaacagtcg taacgaatgc aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca
     6781 gtgaaatatg cagtggacgg gaaagaagaa tccgttactg ctgattatgt gatggtcact
     6841 gtaggtcgtc gtccgaatac agatgatatg ggcttagaac aagcgggtgt cgaagtagga
     6901 gaacgaggat tgatcacggt ggataaacaa ggacgtacga acgtacctaa catctatgcc
     6961 atcggtgata tcgttccagg ggcggcatta gcacacaaag ccagttatga agcaaaaatc
     7021 gctgctgaag caatctctgg caaaaaagta gctgtagatt ataaagcaat gccggcagta
     7081 gccttcactg atcctgaatt ggcttctgta ggtatgacga tcaaagaagc aaaagatgct
     7141 ggtatcgaag caaaagcata taaattccca ttttcaggga acggtcgggc attatcgctt
     7201 ggtaaaacag aaggattcat ccgtctagtt acgacgatcg aagataacgt gcttattggc
     7261 gcgcaaatcg gtggagtcgg cgcaagtgat atggtctctg aattagcttt agcaatcgaa
     7321 tcaggaatga atgctgaaga tatcgccttg acgattcatc ctcatccatc tcttggcgaa
     7381 atcgtcatgg atgcttcgga attagcatta ggtttaccaa tccatatttg atttgaataa
     7441 tacaggcgaa acgcaactga aaaatcagtt gcgttttgtt ttttagaatt atcaataata
     7501 ttattatgta aacttaaaaa tatgacaact tcttggttta ttaccacttc ctatgctaca
     7561 ctgaagagga atatgaaata ggagagttga agatgcaaga atatcagtat ccacttgatt
     7621 tagattggac aacagacgaa atggtcattg taatgaagat gtgggaagat ctggaacgtg
     7681 cgaatgaaaa agggataaac aatgaagctt ttttgaagac ttatcagcag tttaaaactg
     7741 ttgtcaaatc cattggtgag gaaaaacgat tgggacggga atttgaaaaa gcttccggtt
     7801 attctttata tcgtacagtg cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga
     7861 acgggtgaga taaatggaac agatgattga tcatatcaag tcctggcttg aagaagctgg
     7921 tgagaaaatc aaatcttctc aacaaacatt aacaatctcg caaaaatcaa atcgaacgga
     7981 tctagtaaca aatgtcgatc gaaacatcca gaattttcta atcgaaaaaa tccattcctt
     8041 tgatccagag gcgaaaatat tggcagaaga agaagggtgt aatcaattat cggatctcga
     8101 tggaagagtc tttatcatcg atcctattga tggcaccttg aattttgtga ttgaagggga
     8161 aaatttttgt attatgctcg ctgtttatga atccggcgtt ggaaagcttg gtttcattta
     8221 tgacgtgatg agaaatgaac ttttttgggg tggaaaagga ataggcgtgt ataaaaatga
     8281 tgagccattg cctagaccag caaataaatc cttatcggaa ggcttacttg gtgtgaatag
     8341 ttatcttttc ggtcataata aatatggaat ccgagaaatt gcggaaagca gcatggggac
     8401 acgtatgtac ggatgcgctg gtcttgaact agtggcaatc ttgaaaggaa accacattgc
     8461 ttatatttca aaccttagcc cttgggatta tgcagcggga aatgtcttat tggaagagtt
     8521 tggtatggta acaagcggtc tttttggtac tcctttgaca tttgaaaaaa gggaatacta
     8581 tttagcaggg acttcttgtg catatgcaga aatcgcagac cttttgaaaa aagaaggctg
     8641 aaaaaaaggt agaaatcatt cacaaaaatg ataaaaacga gcaaaagatt aattttctcg
     8701 aaaaaatgaa aacattttac tatatttcat gaaaaaagtc tgctataatt agcagtcgag
     8761 taaaatttgc cctgtcataa agaaccaata aaaaaggagt tagttacatt gaattataga
     8821 aacgatatcc gtaacgtcgc aattattgcc cacgttgacc acggaaaaac aacattagtt
     8881 gatgaactat taaaacaatc agatacatta gacgcacata cgcaattgca agaacgtgcg
     8941 atggactcaa atgcgctaga aaaagaacgt ggtatcacga ttttagccaa aaacacagct
     9001 gtagactata aaggtattcg cgtaaacatc atggatactc caggacacgc ggacttcggt
     9061 ggcgaagtag aacggatcat gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat
     9121 gaaggaacaa tgcctcaaac acgttttgta ttgaaaaaag cactggaaca acatatcaca
     9181 ccaatcgttg tcgtaaacaa gattgacaaa ccttctgctc gtcctgagca tgtcgtagat
     9241 gaagttcttg aactattcat cgaattagga gcagatgatg atcagttaga ctttcctgtg
     9301 atttatgcat ctgcactaaa tggtacgtca agcttgtcag atgatccagc agatcaagaa
     9361 ccaaccatgg cacctatttt tgatacaatt attgaaaaaa tccctgctcc tgtcgataac
     9421 agcgacgaac cattacaatt ccaagtttca ttacttgact acaacgatta tgtaggtcgt
     9481 atcggaatcg gacgtgtatt tagaggaaca atcaaagtag gagaccaagt agcattaatt
     9541 aaattagacg gaacagttaa gaaattccgt gtaacgaaat tatttggttt ctttggacta
     9601 aaacgtctag agattcaaga agcaaaagct ggagacttga ttgctgtctc aggtatggaa
     9661 gatatctttg tcggtgaaac agttactcct gtggatcatc aagatgcgtt acctatcctt
     9721 cacattgacg aaccaacatt gcaaatgact ttcttagtta ataattcacc atttgccggt
     9781 cgagaaggta aattcgtaac cgctcgtaag attgaagaac gtttgatggc agaacttcaa
     9841 acagatgtat ccttacgagt tgaaccaaca aactctccag atgcatggac agtttctggt
     9901 cgtggagaat tgcatttgtc tatcttgatt gaaaacatgc gccgtgaggg atacgaattg
     9961 caagtatctc gtccagaagt tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt
    10021 gaacgcgtgc aaatcgatac accagaagaa tatatgggta gtgttatcga gtccttgagc
    10081 ttacgtaaag gtgaaatgca agatatggtc catacaggaa acggacaaat ccgtttgaca
    10141 ttcttaacac ctgcccgcgg tttgattggt tattcaactg aattcctttc aatgacacgt
    10201 ggttacggaa tcatgaacca tacttttgac caatatctgc caatgcttcc tggtcaaatt
    10261 ggcggacgtc accaaggggc gcttgtttcg atcgatacag gaaaagcaac gacttattct
    10321 atcatgagta tcgaagaacg cggtacagtc ttcgtagaac caggtacaga agtatatgaa
    10381 ggaatgatta tcggggaaaa cagccgtgat aatgatttga ctgtcaacat cacgaaagca
    10441 aaacaaatga caaacgtacg gtctgctact aaagaccaaa catctgtcat caaaaaacca
    10501 aaacaattga cgttggaaga atctttggaa ttcttgaatg atgatgaata ttgcgaagta
    10561 acaccagaaa gcattcgctt gagaaaacaa attttagaga aaaatgctcg tgaaaaagca
    10621 tctaaaaaga aaaaataatc tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa
    10681 tagctggttt ctttttttgt ccaactcttt tctgtggaga agtcatcttt gtcaatggac
    10741 ataaattcag aaaatacgcc gaaagacgtt taaaagactt ttaaaaaact tatttctaga
    10801 gagtttttgt ttcataataa gataatgttt cacgaaagga gatcaacttg tccttcgaag
    10861 aaaatcgaag taaaatggca agagaaagga gcgggaagat gaacgactta ttaaaacaat
    10921 actttggcta tgatgagttt agacctggtc aaaaagaaat tatccagaaa gtcattgatc
    10981 aggaaaatgt attaggaatc atgcctaccg gaagtgggaa atcaatctgt taccagcttc
    11041 cagctttact attagatggg cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg
    11101 atcaggtgga cgcggcgaat caattaggta tacccgccac ttttatcaat agctcgttag
    11161 atggttacga aacagctaga cgcttccaag aaatcgatag acagcagtac cgtttacttt
    11221 atatcgctcc tgaacgtttt atcatgccgg attttatcca agcaatgaag cgatggaacg
    11281 tccgtatgat cgctattgac gaggcacatt gtatctctca gtgggggcat gatttccgcc
    11341 caagttattt acagatggcg aaccagctag atcaactgcc aaatcgtcct gtaattgttg
    11401 ctttgacagc tacagcgact gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc
    11461 cagaaaacaa ccatattcag actggttttg aacgagaaaa tctacgattt caagtaatta
    11521 aagatcaaaa aaaagaacaa tatctaatcg aatatttaaa gatcaacaaa aatcaatctg
    11581 gtatcattta tgcagcaacg agaaaagaag tagaccgtct ctatcatttg cttaaaaaat
    11641 tcgatttttc cgtgggtaga tatcatggtg gtctaaatga gaatgagcga acagaaatgc
    11701 aagaagcctt tttatacgat cgcttgcagc tgattgtagc gacaaatgca tttggtatgg
    11761 gtataaataa aagcaatgtc cgttttgtta tccattatca aattcctggc tctctagaag
    11821 catactatca ggaagctggt cgggcgggaa gagatggtct ttccagtgaa gccatcctgc
    11881 ttttctctcc tcaagatatc caagtccaaa agttttttat ccagcagtcc caaagagaag
    11941 aagggcaaaa gcaaaaagaa tatgaaaaat taaaagcgat gacagaatac gtgtatattg
    12001 agtcctgtct tcagcaatac atcctaaact attttggcga gacaagcagt ccgtgcaacc
    12061 gttgtggtaa ctgtttagat gacagagaaa tagtagaagt aacgacagaa gcacagatgg
    12121 ttctttcttg cttaaaacgt atgggtgaga actatggtaa acaaatgctg atgaaagtct
    12181 tagcaggatc gaaagaacaa aagctgcaag ctttaggttt tggacattta tctacctatg
    12241 gactgatgaa aaaccaatcg caaaaagaaa cgatgcagtt aattgaatat ttgatatcga
    12301 atggctatct tctgaccatc aatggtgagt atcctgtttt gaaagtaaca gaaagaggga
    12361 tacaagtatt gaaaggacaa gaatctgttt atcgaaagga acccaagaaa gtccagcagc
    12421 tatcggatga agaaaccgat acactatttg aagtgctgcg ggaactgcga acggatctag
    12481 catcagaagc aggcgttccc ccgtatgtcg tattttcaga ttccacattg aaagaaatga
    12541 gccgaatacg tccatcaagc agattggaga tgcttcagat aaaaggagtt ggccaaagca
    12601 agcttgataa atatggcgaa gcctttttat ctcggataaa aaatgcggac tctaacgttc
    12661 tggataagta gaaagaacga atacgtaaaa atgaataaaa acaactggtt aaatctgttt
    12721 tttttgctcc ttatgaaaat caaatatgtt ataattaagg aattagaatg ggaggtaagc
    12781 acagatggat tctttgtcga aaaaacttgc gatccaagta ttaaatgatg aagcagaacg
    12841 gatcaaacga ttaatccgaa atcaaaacaa cagtctctgt atctcacaat gcaaagcctt
    12901 cgaagaagtg gtagatacac agatgtacgg cttttcccgg caagtcggtt ttgctgtccg
    12961 tataggagta atcggaaaga acgagggcca gctgatgtta agtgagctag aacgagaatt
    13021 gaatcagtta tatagtgagg tttaccaaga aatttatgat aaaaaagaag ttggcaagga
    13081 ggaatagact tgcctaagaa agtgaaaaaa aggcatctat tagactatag catcttgatt
    13141 ccctatttaa ttttatgtgt attgggattg atcatggtat atagttctac gtcgtatcta
    13201 ttattagaaa acggacaaaa tccgtccgca tcagtcatta accagagtat tttttgggtg
    13261 cttagcttga ttgctatcgc actactttat aaaatgaaaa cagatgtttt gaaaaaccag
    13321 cgtttgatca tggcagccat cgcagttttg acgattttgc tgctgattgt tgtgtttttt
    13381 ggtaaggaga tcaatggggc gaaaggttgg ctgcaaattg caggattctc gatccaacct
    13441 gccgaatacc taaaaatcat ttctatctgg tacctgtctc tgactttatc taaaagacaa
    13501 aacagtgtac aaaaagattt tcttgggact gtgaaacgtc cacttgcaat ggttatcgga
    13561 cttactgcat tagtcgctat tcttcccgat tttggtaatg cggcagttat ctttttgatt
    13621 attttggttc tgcttttagc cagcggagtc aattatgtgt atactttgat tgtaggagta
    13681 ggaggctttt gtctaagtac ttttacgatc tggctgatca atatcacaaa cggaaagatc
    13741 ttacccggac gtttacagta tatctataat cgatttgcta tttaccaaaa tcctttctca
    13801 gacgaattaa ataaagggca tcaactagtc aacggctact atgccatgtt caacggagga
    13861 ctatttggca ggggattagg taacagtatc cagaaaaaag gatttttaca agaagcacaa
    13921 accgatttta tttatgcaat cgtagtggaa gaactgggag tgattatggg gattttgata
    13981 ttagcgttgc tattctttat gattgtgcgc attgtccttg taggtatccg ttcaaaagat
    14041 ccgttcaatt cgcttttgtg cattgggatc ggcgcaatgt ttttgatcca agtatttgtc
    14101 aatcttggag gaattactgg cgttattcct ttgacaggga tcacgttccc atttttaagt
    14161 caaggaggct ccagtttgct gatgctttcg atttgtgtcg gctttgtcct caacatcagt
    14221 gcggatgaaa aacgcaagag cttaggttta taataaaaaa agatgtagga gtggttcgat
    14281 gaaaaaagtt ttagttgcta acaggggtga aatcgctgta cgtgtatttc gtgcttgtac
    14341 agaattagga attaaaactg ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg
    14401 tttcaaagcg gatgaagcat atttagtagg acaaggaaaa aaaccgattg atgcgtatct
    14461 agatattgaa gggatcattt ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg
    14521 ttacggtctt ttgtcggaaa acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat
    14581 ttttgttgga cccaaactgc atcatttaga tatttttgga gataaaatca aagcaaaagc
    14641 tgcagctatc gaagcgggga ttgcttcgat tccaggcaca gatggaccaa tcgcttctat
    14701 agacgatgcc ttggagtttg cgaaacaata cggttatccg attatgatca aggcagctct
    14761 aggcggcggc ggacgcggca tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta
    14821 cgaacgtgcg aaaagcgaag caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa
    14881 atacatcgct aatcctaagc atatcgaagt acagattctc ggggatacgc atggaaacgt
    14941 catccatctt tttgaaagag attgttctgt tcagcgccgt caccaaaaag tagtagaagt
    15001 agcgccttgt gtctctatga atgaacagca acgtcaaaaa atctgccagg cagctgtcca
    15061 attgatgaaa tatgtcgggt atgtcaacgc aggtacagta gaatttcttg ttgaaggaga
    15121 tgatttttac tttatcgaag tcaaccctcg cgtccaagtg gaacatacga tcacagaaat
    15181 gattacagat attgatatcg taacaacgca actgttgatt gctcaaggtt tggatctgca
    15241 taaagaaatc ggattgcctc agcaagaagg aattaaatta aatggttcag ccattcaatg
    15301 ccgaatcact acagaagacc cgttgaataa ctttttacca gacacaggga aaatcgatac
    15361 gtatcgttca cctggtggtt ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta
    15421 tgtagtgaca ccgtattttg attcattgct agtaaaagta tgtacgcatg gtgctacctt
    15481 tgaaacagcc attcagaaaa tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa
    15541 aacaaacatt ccatttatgc tcaatgtcat cacacatcct gaattccaat caggaaatgc
    15601 aaaaacaacc ttcattgata gtacagcaac attatttgaa tttcctcgac tacgagaccg
    15661 cggaaacaaa acgatgaaat atatcggtga aattactgtg aatggttttc caggtatcga
    15721 aagtggcgaa aaaccatttt atgaagaacc acgtatgcct aaagatctaa tcacaagaac
    15781 tgattacgta acggctaaga atgtattaga tgcgaagggt gccgatgctt tggtcgaatg
    15841 gatcaaagga caggaaaatt tgttgttgac ggatacgaca tttagagacg cccatcaaag
    15901 tctgctagca actcgtgttc ggacaaagga tttcaagcag attgcacgtt tgactggaga
    15961 aggtcttcct gaactctttt caagtgagat gtggggggga gcaacatttg atgtggctta
    16021 ccgctttttg aatgaagatc cttggcaacg gttgagaaaa attcgttctt taatgcctaa
    16081 tacgttgctg caaatgcttt tcagaggttc aaatgccgtt gggtattcta attatcctga
    16141 caatgtattg gtggaatttg tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat
    16201 ctttgatagt ttgaactgga ctccacaaat ggaaaaaagt atccaagctg tacgggatac
    16261 tggtaaaatc gcagaggcag ctatttgcta tactggcgat atcaacgatc caagtcgggc
    16321 aaaatacaat gttcagtatt ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca
    16381 tatcattgca atcaaagata tggctgggct gcttaaaccg caggcagctt atcgtttgat
    16441 tagtgaattg aaagaaacga cagatctgcc aatccatctg catactcatg ataccagcgg
    16501 gaatggaatc atcacttatt ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc
    16561 aatgagtgca atgagtggga atactagtca gcctagcatg agcagcttgt attatgcact
    16621 ggtcaatggt ccacggctgc cagaaatcac gattgaaaat gcacagaaat taaaccatta
    16681 ttgggaagat gtgcgcatgt attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac
    16741 agaagtatat atgcatgaaa tgccaggagg acagtattcg aatctacagc aacaggcaaa
    16801 agctgtcgga ttaggtcatc gctgggatga aatcaaacaa atgtatcata ctgtcaactt
    16861 gatgtttggc gatatcgtta aagtaactcc atcatctaaa gtagttggag atatggcact
    16921 atttatggtc caaaatgatc ttacagaaga agatatttat gaaaaaggcg aaacactaag
    16981 tttccctgaa tctgttgtga ctttcttcca aggagaacta gggcaacccg ttggcggatt
    17041 tcctaaaaaa ttgcaaaaga ttattcttaa aggcagacct gcattgaatg aacgtccagg
    17101 tcttttggct gaatcagtcg acttcaacga agtaaaaaaa gaattggcag aaaaaattgg
    17161 ctatgaacca aaacaagacg aagtattaag ctatttgatg tatccgcaag tctttttgga
    17221 ctatcaaaaa gcatataatc aatttggcga tgttacatta ctggacacac caacattttt
    17281 ccaaggtata cgtttgggtg aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat
    17341 cattcgttta gatgaaatcg gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa
    17401 tttgaatgga caaagacgtg aaatcactat caaagataat tcgatcatca gtacggtcca
    17461 aacccgattg aaagctgaac cgacaaaccg agaacaaatt ggagcaacga tgtctggttc
    17521 tgttttggat gtattagtga aaaaaggcga taacgttaaa aaaggtgata cgttgatgat
    17581 tactgaagcg atgaaaatgg aaacggccat cgaagcacgt tttgacggag aagtcgcaca
    17641 tgtatatgtg tcttcaggag acactatttc gtctggtgac cttttgattg aagtgactga
    17701 gaaataataa gcgaaaggtg gggaaacatg aaaagactgg gtttgttttg gctgattctg
    17761 ctattagtta ttgtggcatt ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg
    17821 aaggaaaact ctgatcaaca aatatcaaac caaatgacaa cgtggaaata ccaggagctt
    17881 aaagcaactg gtttttctac ttatatcgga aaaccgatcg atcagctgga aaaggatttt
    17941 ggccaagcct atgatcgatt aactagcgga tttggttttg aaacaagata ttatgaaaat
    18001 aaagctagtc atctcacatt tgaagcgaac atccaagaca gcagagtcac aactgttaaa
    18061 gtactgcaaa cagggcctgc tgatatcgcg ccgttttatt tagggatgac gatgcaagat
    18121 cttacgaaaa tcactactat ctatactaat ttcacttttg agtaccatga tacagaagtt
    18181 ggaatcgaac taatggaaga ggatatgaat tatcgtccgc taattgcttt tgataacgac
    18241 acttttgcta ttttattttt tgatcaaaca acaaacggcc tattttcagt tgtgtattta
    18301 gataaagata gtcttttgaa actattgcca tatcaagtgt ttggtgaagg ccttccacat
    18361 tatcaagcag ataaatcggc tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt
    18421 acgactttat taaatatgct tagaagagaa gacgaacttc ccatatacaa tcaaacactt
    18481 gcctttcaaa accacagtca gctgctacta catgattttt tgacaaggcc tgaggaaata
    18541 ttgtcagagg aacgtatggc tgattggcaa aaatcgctag gcagtgctca aacgacagtg
    18601 aaattctcac tgacgaatga tgaattggag aagttaatca gtaaacaaaa attagaaaat
    18661 actagtgggc tatttattca tccggcgatt gacgctactt tttcgttttt atattggtac
    18721 agcgatcctt acaaccatga ccgtttcatg gacccgggaa cggattctgt aggcattgct
    18781 ttttctaaag aaaatatgtt agtcttattg caagaagaaa acggagaaag tagtgatagc
    18841 agtgatcgtt aacgaagagc tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat
    18901 gataaaaaag agtaaaacga tggcttctta tcttgaacat aaacagtcaa tggaagaatc
    18961 tgcaaaagtt tgccagttga aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt
    19021 agcggcatat ggtgagtatg cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc
    19081 aaaaagacag cttgatatgg atgagcatgt tgcagctttc cgatatagtg aaacacagct
    19141 gcaaagtctg ttggatcaaa ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa
    19201 agtagatgca ggtagcccgt tttttgaaac gaaaaaacac tttggatgcg gaggtaattg
    19261 tcatgcaatt agacaatagt gaatttgaaa taacaaaacg acgcgggctg atcgtatggg
    19321 tctacagctt aaaacaatta aagaatctga aaaagttcgg cttgttgcat tatgtctcaa
    19381 gaagaatgaa atatgtcgtg ctttacttaa atgaagagac attggaagaa acagaagaac
    19441 gaatccgtaa attacatttt gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa
    19501 tgaattttgc cgaaaaaata ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg
    19561 aagagctaaa tacagaaatc cgattagctg aacatcttta aaaatctctt atgagcagtt
    19621 tttaaataaa aaataacttg gataacgatc gatttttgat cattgtccaa gtattttttt
    19681 ggtatactag taccagtacc atttctaagg agtgagtaga tgcggataat atcaggaaat
    19741 tatggcgggc gccgtttaaa aagtttagca ggggcgaaca ccagaccaac tacagataaa
    19801 gtaaaagaat ccatattcaa tatgattgga ccatattttg atggagagac agtattggat
    19861 ctgtttgcag gaagcggtgg tttagctatc gaagcagtat ctagaggctg cagtcatgcg
    19921 gtatgcgtag acaaaaatta ccaagcgcta aaaatcatca aagaaaacat cgaaattacg
    19981 aaagaaccag agaaatttac cacaataaaa cttgatgcgg acaaagcaat cgaacggcta
    20041 gcagcggaga aacaacaatt cgactacttg tttcttgatc cgccatatgc aaaacaaaaa
    20101 ataatcgatc aaatccataa aatggaagaa ctgggacttt tttctaaaac cgcagttatc
    20161 gtatgcgaaa cggataaaac agtcgaattg ccagaaactg tggctggatt ccatcaaata
    20221 agaaaacaga tttatggcat aacggccgta actatctata gaaaagaggg aaatcaatga
    20281 aacgtgcatt attcccagga agttttgatc cttttacaaa aggtcactta gatacggtgg
    20341 aacgtgcggc taaattattc gatgaggtag taattggtgt ttttatcaat acttcaaaga
    20401 aaagtttgtt tcccccagaa gaaagaatga cattgatcac aaaagctgtt tctcatcttc
    20461 ctaatgtaaa agtcatgcat caagaaaatc aattgacggt agaaacagca aaagagattg
    20521 gtgcagatgc gttgatcaga ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga
    20581 ttgctcagat gaatcatcat ctatatgatg aattggaaac tgtcttctta ttagcaaaac
    20641 cagaatatag tcacgtgagt tccagtattt taaaagaagt actgcacttt ggcggagatg
    20701 tgagcagcta cttaccgcca gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg
    20761 aaagctaaac aatggttgaa aacagtactg attatcatat ttggaatgtt gatcattgga
    20821 tttgtagtag ttcccttgcc gtattatgta gaatcaccgg gagccacgat cgatctgaaa
    20881 gaactaatta cggtcaatga taaggaagat aaaggttctg ggtctttctc tcttacctct
    20941 gttggtatca ggcaagctac aggtttctta gctttaaaag ctaaaatgac cccttttgaa
    21001 gaattgatca gtgaagaaga attgactggt ggggcgacaa atgaagaata taatcaaatg
    21061 caacagtatt atatggattc ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca
    21121 gacaagcctt ataaaatgga attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg
    21181 aatttctttg gcaagctttc agttggagat acagtaacaa aagtagacgg gaaaagcttt
    21241 caaagtacag aagcatttat ggattatgta aaaagccaaa aagttggaca aacaatcgaa
    21301 atcagttatt tgcataatgg tgaagaaaaa acagcttctg gtgatctgat cgagttacca
    21361 acagataaaa aggcaggaat aggtatcacg cttactgacc ataccgagat cgaatcagat
    21421 acagatgttc gtattaattc tggctctatc ggtgggccat cagcgggatt gatgttcact
    21481 ttagaaatct atgaacaagt cactggtaaa aacttgcgac atggtaagca aattgcagga
    21541 actggaacga tcaacagtga aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg
    21601 gcaagtgcgg ataaagctgg cgtagagatt ttctttgcac cagatgatac gatttcagac
    21661 gatgttaaga aagaatatcc tgaaataaaa accaactacg aagaagcaaa agctgccgct
    21721 aaaaaattga attcctcaat gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac
    21781 tatctagaga aaatgaaatg aaactaaata attaaatagg ggttgtgaca cgagttctgt
    21841 cacaacccct ctttttcgtc aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt
    21901 gacgtaatta gatgttgaag ggacgatttt gccagagctc actgtttctt caattttaaa
    21961 tgaaaggagt aatgaatgat ttttactggt cattctttga aaaaagaaca atgatagaaa
    22021 aaatcagcaa gaaaatacta gcttcaccca aattgatcat ttggatgtct atgatagttc
    22081 ttatcttttg tatcgcaagt ggttttttat tatcaaattt gctattttat agagaaacag
    22141 aagagactgc tgcaaaagaa atactttctt cgtttagttc cagtggagaa gatccatcag
    22201 ctgaaggaaa cgatgtagaa gaggatgctc gtccaaaggt catgtacacc gatatcaaag
    22261 gaagtgtgaa ggagcctggt atctattcct tttcttcaga agaacgggtc tatgatgtac
    22321 taaagcgtgc aggtggatta ttagaagaag cggatagcga tcgaatcaac ttttctgcga
    22381 agatagaaga tcagcaagtc ctatatatcc ctgctgtagg tgaagagctg ccagaacatc
    22441 ttgatcagtt ggcatcctcg gaaggaaaac agcctacagc agattccgag cctcccaaaa
    22501 taaatatcaa tacagccact ccatcagaac ttcagcagat tcctgggatc ggatcagtca
    22561 aagcgcaaga aatcattcga tttagagaag aaaacggatc cttccagaaa gtggaagacc
    22621 tgcaggagat ttctggtatc ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa
    22681 ttaaatagaa tgattcaaaa tcgactggca cgagcaataa atggacgcta atggatacta
    22741 agaataatcc aattctaaga gcttggcagg acgaggaggt agtatttttg gataggaaat
    22801 agcttgattg ttcgcaaaga aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat
    22861 aaaatatctg ggattcatat tatgaatacg gctgtcagcg gcaataacag tcaagctcta
    22921 cgagaacgct ttgccaaaga tgtacttagc tatacagcag ataaagtatt tattctcatt
    22981 gggacgaatg atctagcaga gcataaacaa ctttctaaag aaacgtacca aaaaatctgc
    23041 agtggatgat agttcagctg aaaaaagact atagttctga tcatgctttc tttatcacgc
    23101 caccagcagt cgatgagaca aaacaaaaat atcggaataa caagttgatc aaagaatata
    23161 tctctgttct cacagaagta gcaaaacgcc atgattgtga agtatcgatt tttatacgga
    23221 atttcttgaa caaatggatt cgaagaattt ttataatggg ctaatggatg acggacttca
    23281 tttcggcagt gaagggtagg atcttttgtc agacaggatg attcgttata tttaaagaat
    23341 attttgttta agcaatgagt actttttaat caacttgaaa aaacggagga atataaaaca
    23401 tgttagaaaa atcttcaaaa gcatgcgata cgaataaaga aaaagttact gataagtcac
    23461 ataatgaacg cattccttgg gaccagtact ttatggctca ggcagtactg ttatctttac
    23521 gtagtacgtg tacacgttta gaagtaggag cgacactcgt tcgagaaaaa aggatcattg
    23581 ctggcggcta taatggagca gtcagtggag atgtccactg catagatgaa gggtgttacg
    23641 tcgtggatgg acactgcttg cgaacgatcc atgcagagat gaatgcactc ctacagtgtg
    23701 caaaacttgg tatcccgacg gaaggttcag aaatctatgt cacacatttt ccatgtttgg
    23761 cctgtacaaa ggctttgttg caagctggtg tgaaagggat caattatctc catgactatc
    23821 gaaatgatcc ttatgcccaa gcgttgatcg aacaattagg tgtatctgta caccaagttg
    23881 ctttagactc acagcatttt tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc
    23941 gttttagaaa ggcatgttcg aaatcggttg atttttccgg caattacgtc gatgattatc
    24001 tgtttgttgt attacactga caattggggt tatcgtttgc ttcttgccgg aattttttta
    24061 tggttttcgc taaaagaagg acgttttatc gcgtacttgt gtattttgtg tagcataacg
    24121 gtatccattt cttataggct acaagagtat tcatttttag aggaaaaaac aacatctgcg
    24181 caaattactg tatttacaga tacgattcgt gcaaatggta attttttgac aatggaagga
    24241 aggttatcag agaataaaca aaagctccag ctttcttatc aagtaaacac agaagcagaa
    24301 ttgaaaacat ggcttaactt ccgaggaaag ggaatagaat tgactgctga aggcgagttc
    24361 atctctccag caaaaaagcg gaatctttat aattttgacc aagaagctta cttcaggaac
    24421 catagaattt ctggacggtt ccaaattcaa aagattgaac agatcaccat gcaaaatcac
    24481 tggcgaaact ggcttcagag aaaaagagga tcttttatct cctttgtcca atctgaattt
    24541 ccgcaaaaaa caagtgtata tattaattct ttattatggg gctataaaga tgcaggattc
    24601 agagaaccag aagaaatttt tagggagagt ggtttgctgc atctgttcag tctatcgggt
    24661 ctacacatcc aattttattt agggtggctg tattatttgt ttcgacgtat cggatggcca
    24721 ctacacgtca gtattattcc gttgagtcta ctaactatct gttatgtttc tattgcagga
    24781 agttctattt ctgttttgcg tgctagtttg ctgtttttat tgcgattgct agtcaaattg
    24841 ctgaataagc attattccac catggactta tttgcagttg tcttttggct tcttttacta
    24901 tttgatcctc gatttctttt tcatgcgggt ggacagctta gttttttcat gagttttttg
    24961 tttatcctga tttcttttga tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt
    25021 actatgatca cgatgccgtt gattgtatgg catttttatg agtggcctat tttaggtagt
    25081 ctttttacat tactgattgc accggtattc aagtttcttt ttcttcctgg tgtgttattt
    25141 ctcacactct tcaatcgctg gctgccggaa actttacttt tgctgataga agagggatta
    25201 atcttatttg agcgaataat cgatttttct tcagggtcgt ctctcattat aggacgtttg
    25261 ccccttgtgc tatctgggat gtatattttc ggattactga tttgtatgga taggttgaaa
    25321 gaacaacctg tgaaacactt gttttatgtt ggtctttttt cttttatttt tttgactttg
    25381 ccatttttac gcttttcttc aacgatcgct tttatcgatg tcggtcaagg agacagtatc
    25441 tttttgcaaa gtccttttcg aaaagaaacg attttgatcg atactggagg tcagttacac
    25501 tttgaaagag aaaagtgggc gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt
    25561 ccatatctaa aaggtcaggg gataagtgta atcgataaac tcatcttgac acacgacgat
    25621 acggatcatg tgggagaatt acccacgtta tctcagcatt ttactatcaa aaaaatttat
    25681 atcggttggg gcgctggacg aaatgagcca ttacaaaggc atttatctga agctcaaaaa
    25741 aatggtactg agatcattga aatcaaacat ggggacagga tcagcggtta ttatgatctt
    25801 tatgtattga ctccttttga aaagggagag ggaagaaatg aagattcgat tggtctatgg
    25861 atggaatata acaataggcg ctttttattt ttaggagatt tgaatcaagc aatggaaaaa
    25921 caattgcttt tgatttatcc taatctgaaa gccgatgtag tcaaattagg tcatcacggt
    25981 agccgtacgt caagtaacac agattttctt tcccatattg aagcaaaaca tgggattata
    26041 tcctgcggcg taaataatcg ttatggtcac ccgcacagcg aagtattgga gacactagaa
    26101 gaaaatcaga taaaaacatg gcgaacggat caacaaggaa tgatcaccta tcgctggcat
    26161 ccagtttttc atcctcatgg gctagtggaa acaatgatag actagtaatt ctcatctgct
    26221 catggtagga taatttttga aagtggtgag taatttgagt atacaagcag agttacaaaa
    26281 aatacgtaaa gaaaaacttg catcttgtta cttgatttta ggaacagaaa agtttctcca
    26341 agatcaagtt cggacagaaa ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa
    26401 tttttta
//
LOCUS       DASJON010000015        26036 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJON010000015 DASJON010000000
VERSION     DASJON010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.015"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(218..1015)
                     /locus_tag="U3989_000488"
     CDS             complement(218..1015)
                     /locus_tag="U3989_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN1713958.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="U3989_000489"
     CDS             complement(1012..2196)
                     /locus_tag="U3989_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1713959.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="U3989_000490"
     CDS             complement(2221..3468)
                     /locus_tag="U3989_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1713960.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="U3989_000491"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="U3989_000491"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN1713961.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="U3989_000492"
     CDS             5448..5762
                     /locus_tag="U3989_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN1713962.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="U3989_000493"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="U3989_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN1713963.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="U3989_000494"
     CDS             6447..7091
                     /locus_tag="U3989_000494"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN1713964.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="U3989_000495"
     CDS             7106..7435
                     /locus_tag="U3989_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN1713965.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="U3989_000496"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="U3989_000496"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN1713966.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="U3989_000497"
     CDS             8423..9247
                     /locus_tag="U3989_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN1713967.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="U3989_000498"
     CDS             9240..9587
                     /locus_tag="U3989_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN1713968.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="U3989_000499"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="U3989_000499"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN1713969.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="U3989_000500"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="U3989_000500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN1713970.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="U3989_000501"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="U3989_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN1713971.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="U3989_000502"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="U3989_000502"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN1713972.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15217..16011
                     /locus_tag="U3989_000503"
     CDS             15217..16011
                     /locus_tag="U3989_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1713973.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="U3989_000504"
     CDS             16001..18043
                     /locus_tag="U3989_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1713974.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="U3989_000505"
     CDS             18324..18473
                     /locus_tag="U3989_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN1713975.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="U3989_000506"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="U3989_000506"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN1713976.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="U3989_000507"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="U3989_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN1713977.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="U3989_000508"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="U3989_000508"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN1713978.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="U3989_000509"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="U3989_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN1713979.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="U3989_000510"
     CDS             22890..23972
                     /locus_tag="U3989_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN1713980.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="U3989_000511"
     CDS             23972..24775
                     /locus_tag="U3989_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1713981.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="U3989_000512"
     CDS             24845..25585
                     /locus_tag="U3989_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1713982.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..>26036
                     /locus_tag="U3989_000513"
     CDS             25596..>26036
                     /locus_tag="U3989_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN1713983.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAH"
BASE COUNT         8152 a         4640 c         5405 g         7839 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcat
//
LOCUS       DASJON010000016        25796 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJON010000016 DASJON010000000
VERSION     DASJON010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.016"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(109..1407)
                     /gene="eno"
                     /locus_tag="U3989_000514"
     CDS             complement(109..1407)
                     /gene="eno"
                     /locus_tag="U3989_000514"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN1713984.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1523..2278)
                     /locus_tag="U3989_000515"
     CDS             complement(1523..2278)
                     /locus_tag="U3989_000515"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN1713985.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2382..3572)
                     /locus_tag="U3989_000516"
     CDS             complement(2382..3572)
                     /locus_tag="U3989_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN1713986.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3682..4683)
                     /gene="gap"
                     /locus_tag="U3989_000517"
     CDS             complement(3682..4683)
                     /gene="gap"
                     /locus_tag="U3989_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1713987.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4724..5815)
                     /locus_tag="U3989_000518"
     CDS             complement(4724..5815)
                     /locus_tag="U3989_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN1713988.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5964..7298)
                     /gene="rpoN"
                     /locus_tag="U3989_000519"
     CDS             complement(5964..7298)
                     /gene="rpoN"
                     /locus_tag="U3989_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN1713989.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7358..7429
                     /locus_tag="U3989_000520"
     tRNA            7358..7429
                     /locus_tag="U3989_000520"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7391..7393,aa:Arg,seq:ccg)
     gene            complement(7621..8505)
                     /locus_tag="U3989_000521"
     CDS             complement(7621..8505)
                     /locus_tag="U3989_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1713990.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8592..9491)
                     /locus_tag="U3989_000522"
     CDS             complement(8592..9491)
                     /locus_tag="U3989_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN1713991.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9508..10014)
                     /locus_tag="U3989_000523"
     CDS             complement(9508..10014)
                     /locus_tag="U3989_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN1713992.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10203..10823)
                     /locus_tag="U3989_000524"
     CDS             complement(10203..10823)
                     /locus_tag="U3989_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN1713993.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10899..11336)
                     /locus_tag="U3989_000525"
     CDS             complement(10899..11336)
                     /locus_tag="U3989_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN1713994.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11499..12329
                     /locus_tag="U3989_000526"
     CDS             11499..12329
                     /locus_tag="U3989_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN1713995.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12313..13680
                     /locus_tag="U3989_000527"
     CDS             12313..13680
                     /locus_tag="U3989_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1713996.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13699..14508
                     /gene="yidA"
                     /locus_tag="U3989_000528"
     CDS             13699..14508
                     /gene="yidA"
                     /locus_tag="U3989_000528"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN1713997.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14551..14994)
                     /locus_tag="U3989_000529"
     CDS             complement(14551..14994)
                     /locus_tag="U3989_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN1713998.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15128..15499
                     /locus_tag="U3989_000530"
     CDS             15128..15499
                     /locus_tag="U3989_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1713999.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15668..16450)
                     /locus_tag="U3989_000531"
     CDS             complement(15668..16450)
                     /locus_tag="U3989_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN1714000.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16447..17418)
                     /locus_tag="U3989_000532"
     CDS             complement(16447..17418)
                     /locus_tag="U3989_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN1714001.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17510..18904
                     /locus_tag="U3989_000533"
     CDS             17510..18904
                     /locus_tag="U3989_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1714002.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(19096..20451)
                     /locus_tag="U3989_000534"
     CDS             complement(19096..20451)
                     /locus_tag="U3989_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN1714003.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(20481..21116)
                     /locus_tag="U3989_000535"
     CDS             complement(20481..21116)
                     /locus_tag="U3989_000535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN1714004.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(21123..22499)
                     /locus_tag="U3989_000536"
     CDS             complement(21123..22499)
                     /locus_tag="U3989_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN1714005.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(22492..24273)
                     /locus_tag="U3989_000537"
     CDS             complement(22492..24273)
                     /locus_tag="U3989_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN1714006.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(24295..24606)
                     /locus_tag="U3989_000538"
     CDS             complement(24295..24606)
                     /locus_tag="U3989_000538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN1714007.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(24596..25582)
                     /locus_tag="U3989_000539"
     CDS             complement(24596..25582)
                     /locus_tag="U3989_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN1714008.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            complement(25597..>25796)
                     /locus_tag="U3989_000540"
     CDS             complement(25597..>25796)
                     /locus_tag="U3989_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN1714009.1"
                     /translation="YLSQTLLDEWNNELPFMMVLSDETVADQAGFLINDQGVQYNFLF
                     SSLVQDIQGTMSFEIANQLFE"
BASE COUNT         7267 a         5496 c         4574 g         8459 t
ORIGIN      
        1 actctctacc tgctgatatt tcagcaagta gagagttcga ttacttaact aagtgatcat
       61 actcgactat ctttcaatag tcgatcatgt atcttcatta gaaaagaatt atttgttttt
      121 taagttgtag aaagatttca aacctttgta ttctgcaact tcaccaagtt ggtcttcgat
      181 acgtagtaat tggttgtatt tagcaatacg gtcagtacgt gatagagaac cagttttgat
      241 ttggccagca tttgttgcaa cagcgatatc agagattgtt gaatcttctg tttcaccaga
      301 acggtgagat acaactgctg tgtagccagc ttctttagcc atttcgatag cttcgaaagt
      361 ttctgttaat gtaccgattt ggttcacttt gataaggatt gagttagcaa ttcctttttc
      421 gataccttca gataattttt gagtgtttgt tacgaacaag tcgtcaccaa ctaattgaac
      481 tttgtcgcct aatacgtcag ttaatttctt gaatccgtcc cagtcgtttt cgtctaatcc
      541 atcttcgatt gagatgattg gatatttaga aactaattct tcgtagaatt tgatcatttc
      601 gtcagttgtt ttttcgcctt cgcctgagtc agctagtacg taaacacctt tttctttgtc
      661 gtagaattct gaagaagcag catccatagc aagaacaacg tctttaccag gtacatagcc
      721 agctttttcg attgcttcga tgattacttc aaaaccttct tcgtttgaac caaggtttgg
      781 agcaaatcca ccttcgtcac caacagaagt tgctaatccg cgagctttca agattgaagc
      841 taatgcgtgg aatacttcag cacccatacg tagagcttct ttgaatgttg gagcgccaac
      901 aggcatgatc atgaattctt ggaagtcgat tgagttatca gcatgtgatc cgccgttgat
      961 gatgttcatc attggtgttg gcaatacttt tgtgttgaat ccgcctaggt agtggtataa
     1021 aggtacttct aggtagtcag cagctgcacg tgcaacagcg atagaaacac ctaaaatagc
     1081 gttcgcacct aatttacctt tgttaggagt tccgtctaaa gcgatcatag ctttgtcgat
     1141 agccatttgg tcacgaacat cgtagccaat gattgcttca gcaatgatgt tgtttacgtt
     1201 gtcgacagct ttagttacac ctttaccgcc ataacgagct ttgtctccgt cacgtaattc
     1261 aactgcttcg tattcaccag ttgaagcacc tgaaggaacc attccgcggc caaaagctcc
     1321 gctttctgtg tatacttcta cttcgattgt tgggttacca cgtgagtcta agatctcgcg
     1381 agcgtaaaca tcagtaataa ttgacatgtt ttgtctctcc tttgagtttg ttttttccta
     1441 agggatttcc ctcatatacg attttagtta attttgctca ttagtgcaaa catttcttgc
     1501 atttttcgca aattttcaac aattatttta cagcgtctaa caatgacaaa aatgaatctg
     1561 cttgcaagct tgcgccaccg actaaagcac cgtcaacatt ttcttttgcc atgtattcag
     1621 caatattttc tggttttaca gaaccgccgt attggatacg aactgcttct gatacttctt
     1681 ttccataaag tttttcaact gtttgacgta caactccaca aatttcgtca gcgatttgtg
     1741 cgtcagctga tttaccagta ccgattgccc agattggttc ataagcaata accatgtttg
     1801 acacttgttc agctgaaaga tcagctaaac cagctgtaat ttgtccttcg atccattcag
     1861 cagttttgcc tgcttcatag gtttctaaag attcgccgca gcataggatt ggtgtcatac
     1921 catttgcaaa gatagctttt gcttttttat tgatgtcttc gtctgtttca tggaaatatt
     1981 cacgacgttc tgaatgtccg atgatcacat aatgaacacc tagatcagca agagctgctg
     2041 gtgaagtttc accagtgaac gcacctgagt tttcccagta gcagttttgt gctgagattt
     2101 ttagttctgt accttcagct gatgtcatta attcttgtaa gaataatgca ggagatccga
     2161 tgactgaatc aactttgtca tttgaaggga ttttgttttt tactgcttca gcaaattctt
     2221 ttgcttctga tgcagttttg ttcattttcc agttacctgc gataataggt ttacgcattg
     2281 aggaacacac tccttaattt attgaaaaat ggctagggag tcatgaaaac tcccttcacc
     2341 ttttgatcga ttactcgtca gaattgagca aacttggtcg tttatttatc gttgattgct
     2401 gctagtcctg gtaattcttt accttcaagc aattccaagc ttgcgccacc acctgttgag
     2461 atgtgtgtga atttgtcagc aaatcctaat tgttctgctg ctgcagctga gtctccgcca
     2521 ccgatgattg ttgttgcgcc ttctaagtta gcgattgctt cacaaacacc gattgtacct
     2581 ttagcaaagt tactcatttc aaatacaccc ataggtccgt tccaaacaac tgtttttgca
     2641 ccttgtaatg tatcagcaaa taattttact gatgctgggc cgatgtctaa agccatcaaa
     2701 ccatcaggaa tgtctccttc gattacttgt gtttcaacat catttgaaaa ttctggtgca
     2761 cagacattgt cgattggcaa gacaagtttg tcgcctgctt tttcgattaa ttcttttgct
     2821 agttccactt tatcagcttc tactagagag ttaccgatct tgattccttt tgcttcgtag
     2881 aatgtgtaag tcattccgcc accgatcaag attttatctg ctttaggaat caagttttcg
     2941 attacgccga ttttatcaga aacttttgcg ccaccaagga ttgcaatcat tggtcgtttt
     3001 ggttcttcta ctgcttcacc gatgaacttg atttcttttt ccatcaagaa tccagcaact
     3061 gttgggatac cagttgaagc gatacctacg ttagaagcat gtgcacggtg agctgtaccg
     3121 aaagcatcgt taacaaatac atcgcctaat gaagcccagt atttacctag ttctgtatca
     3181 tttttgcttt cttttttacc gtcaatatct tcaaatcgtg tattttcgaa tacgacaacg
     3241 tcgccgtcat tcatgttgcg gattgcttct tctaattgtt ctccgcgagt ttcaggtacg
     3301 aatgttactt ctttacctaa taattcgcct agacgttttg caactggagc taaagatttt
     3361 ccttctttat cttcttctgt ttttacacgt ccaaggtgag agaaaaggat tgcttttccg
     3421 ccttgttcta gaacgtagtt gattgttggc aatgctgctt tgatacgagt gtcgtcagtg
     3481 atcacgccgt ctttcaaagg aacgttgaag tcaacacgga caagaacttt tttgtctttt
     3541 aaatcgatat ctttcactgt tttcttagcc attttatttc ctcctgaata aacttattat
     3601 caaaaaaagc ggaagaagcg cgattgcttc cccgctttta aatgtacatg tctataattt
     3661 acacttgtac gtttgtctat cttataagtt agcgaagtat tctaaagtac gtactaattg
     3721 tgcagtgtat gacatttcgt tgtcgtacca agcaacagtt ttaactaatt gtttgtcgcc
     3781 aactgtcatt actttagttt gtgtagcatc aaataatgaa ccgtaagtca taccaacgat
     3841 atctgaagaa acgatttcgt ctgtgttgta tccgtaagat tcgtttgaag cttctttcat
     3901 tgctgcattg atttcgtcaa cagttacttc tttttcaaga actgttacta attctgttaa
     3961 tgaacctgtt ggtacaggaa cacgttgagc agctccgtct aatttaccgt tcaattcagg
     4021 aattactaaa ccgatagctt tagcagcacc tgttgagtta ggaacgatgt ttgcagcagc
     4081 agcgcgtgca cggcggaagt cacctttagg atgaggtccg tctagagtca tttggtcacc
     4141 tgtgtaagcg tggatagttg tcattaatcc ttcaacaaca ccaaatttgt cgttcaatgt
     4201 tttagccata ggagctaaac agttagtagt acatgaagca cctgagataa ctgtttcttt
     4261 accagttaat gtttcgtggt ttgtgttata aacgattgtt ggaacatcgt ttccgccagg
     4321 agcagagata acaacgcgtt tagcaccagc tgttaaatgt ttttcagctg ctgatttaga
     4381 agtgaagaaa ccagtacatt ctagaacgat atctacgcct agttcgcccc atggtaattc
     4441 ttctgggttg cggttagcta atactttaac ttcttttccg tttacgttga atgaaccttc
     4501 atgaacttca actgttccgt tgaaacgtcc ttgagttgtg tcgtatttta acaagtgtgc
     4561 caacatttta gcatctgtca agtcgttgat tgctactact tcgattccat ctacatcttg
     4621 gatacgacgg aatgctagac gtccaatacg tccaaaacca ttaataccta ctttaactgt
     4681 catttaagat ttcctcctta tgaaaatcaa aaaaatattt attttaaagg gttacccctt
     4741 ttaaaatctc atttgcagca gcttcatcag tgatgagcca cgtttgtttt ggtgcatttt
     4801 tcatataagc tcgaattgct ttagcttttg attttccgcc cgcgattgcc atgatgatcg
     4861 ggactttttc caaatcttgc aattctatgc cgattctagg gaccttataa acgacttcac
     4921 ctttttcatt gaaaaagtag ccaaaagatt ccgcaacagc atttgcttgt ttcagcatga
     4981 ctaattcttt ttcagtcatc ttacgacgtg ctgccatgtg taatgcacgc ccaatactat
     5041 gaataacaca attcgcttca ctaatcaagt ttaagacttc ttggatagat ggctcattga
     5101 gtaaagaatt gtaagttgcc agacttaatt gttccggaac ataaagtgca cggtggttac
     5161 cgtttgcttt agcagccatt cttgcactga cagaatttgc ctgtacagtt atcgcttcac
     5221 caatcccgcc tcttgcaggg acaaacaggt tatgccgttt cttattttcc agattagaca
     5281 attgttcggc taccacagcc atagtagttc ctcccatcac agcgatgata ttctcgcctt
     5341 ctggaagtag tcggtctagg gcttcgctca gcgcttcgcc gaatgcatca gccactttgg
     5401 tctgctcttc gctgtttcct gaaacaacga tacagcgttg gttaccaaaa tattctgcca
     5461 actgttgttc ggtttgatgc gtacctaaaa gctgatccat gaagttttca aggctttgat
     5521 aaacttcttc gcccttagtt gtcagcgtca taccgctttt tgaggtgtcg atcaaattca
     5581 gatttttcaa aagatctgtt tctgttcgca atactcgttc tgtgatcccc atactttccg
     5641 ataaacttcg acgtccaatc ggctgcatcc aatagatatt tcgtaaaatt ttatagcgtt
     5701 cttgcaacac ttcgataata tctggtgcaa cagattcaat catttttatt tcatccagca
     5761 tagcgatctc cttagttgga cactttacgt ccaatgtaga cttaaaacga cccatatacg
     5821 ataaaaaaag aaattaacta ttcgaatttc acatcaataa agggaaatct caacctcacg
     5881 actgtaagtc taacagtttt gctctcgttt ttcaactaaa aagactagaa aaagtgaaat
     5941 actttttcta gtcttttgac tgattagtta tcataacgtt ttctttttga tgaactcggg
     6001 attcctaata aatcacgata ttttgctact gttcgtcttg agatcgctat ctcatctttt
     6061 ttcagcaatt caaccaattt ctggtcagac aacggcttgt ttttgtcttc ttgatcgact
     6121 aattcctgca gctttttctt cgctgtatct gctgaaaggt cttctgtctg ttctgtacta
     6181 ttcgtaggaa ttcgtgtagt gaaaaatttt tttaattcaa atacaccgaa gtctgtttcc
     6241 aaatatttcc cgttgactgc acgactgacc gtggattcat gcacatctat ctcagttgca
     6301 acttccttta acgtaagagg cttgattgga cgttccttgt cgataaagaa agctttttgc
     6361 cgggaaacaa tgacttgagc aacccgcaaa attgtatccc cacgttgtag gatcgttttc
     6421 ttcaaccatt cgaattcctg tttccgttct tttaaataat tttcagtttc tttatctgct
     6481 tgttgtgaca tttgtttgaa gtaactctct tgaaagcgta tatttggctg attttttcgg
     6541 tttgagataa cttgtacatt tttatcgtcg tccactaata cccggacatc aggaataatg
     6601 tacaattcgc tgctacggtc gaaaatccgg ccagggcttg gactcaacgt ttgtaaatag
     6661 tcgaatatct gctgaacagc atgtagatca acagaaaaac gttgtgcgat ttctttccat
     6721 tttcgctcca ccagttcctc aaaacattct tccaacacga tataagctaa ttccggcgca
     6781 taatcatctc gttctgtttg aagcatgaga cattcttgca aactacgtgc acctactcca
     6841 gctggttcta attgctgaat cagagtcaac gcatcgagca tttggatagg cgtagctcca
     6901 atctcttttc cggcttcttc taaactaatc gtcaaaaatc cgttcaaatc gatgtattct
     6961 acaagataaa gaacgatttt cctcaaaaaa gtatctcggt aattcaaatg gacttgttcg
     7021 atcaatgaat caaataaaga cccttttgta tcaggaatct gatttaggta atttgtctct
     7081 tctccttcat aagaagatga agaagctttt atgtaatctg gctgccattc cggctcaaca
     7141 acttccacca atggattttc cattgcttga ttctcaacga atgccaacag ctcctcactg
     7201 ttatattgta aaacttggat ggactgttgc aactgttgcg tcatggctag tttctgtgtc
     7261 tgctgctgtt tttgtgaaaa attttgctca aatttcattt ttttgtccaa tccccttgta
     7321 attttttatc tgtttagtta caattatccc agtgcttgcg tccttagtgt agtggatatc
     7381 acgtaagatt ccggttcttg agacgggggt tcgattccct caggacgcgt tctactgcta
     7441 ttttagcata atttacgaat agttgcttct ttatttttaa gtgaaaactg tccgccaaag
     7501 aaaattggtg gacagttttt tgttgtacct gtatagatat tgttaagcct ttgcaagtaa
     7561 ggtagttatg tgttcaacta cttattaaaa tgtgttattt attaaagata cttgtctcac
     7621 ttactgaact ggccgatgat cgtttctgcc ataatagttc tcgacagccg ttggatgccc
     7681 taataaatcc ccaactgtcg tgatgcgata cccttgctct ttcagataat cgatcacttg
     7741 aggaacggca cgaatcgttt caggatagat atcatggaat aaaataattg ccccatcgta
     7801 tactgttgct tggatcttct gcaaaatcaa atcaggattt cttgtcttcc aatcttctga
     7861 atcaacagac cattcgatga tcggtcgctg gatgatcgtt gctcctacat tcgtgacaga
     7921 accatagggc ggacgaacaa aagtgggggt tttgccagtg acttttttga tctcagtgtc
     7981 tgctttcgtc acttcctccg tcatcgactg ggcagaaaga gtggccagat tctgatgatc
     8041 gtatgtgtga ttgccaatct cgtgccctgc ttcagctgtc tgcttcacga tttcaggatg
     8101 ctgcgccgta ttttctccta agacgaaaaa agtggcatgc acgttttttt cttttaagat
     8161 tcgcagaagc tgcggtgttg tggtcgctcc tggtccatca tcaaagctga ttgcgacgct
     8221 tttgacagcc ggatctcggc tcgtttttgt actgtcttgt gttgattgtg atgactgact
     8281 ttgtaccttt tccgctgatt cttgaatggc ttctttgacg ttagctgttt cagttgattg
     8341 gcttttaatg gttgattgtt ctgattgttt gaaagattgt gacgaatgct ccgaagagtg
     8401 tgtttgtcct tttacttcgg tgatcaagga ggccaccgaa gtaatttcga aaaacaaaag
     8461 cacggataaa gcaataatac tgataactaa ataatttctt ttcactatat ctcttctttc
     8521 tctttttctc ttcttattat ttacttgtac ttacttgaaa attgaaactg ctagtcgcaa
     8581 ctacttctcg tctaatgatc caaataatct tccgatgcca aaatcgtttt tgtcacggac
     8641 ttaggaatga caaattctgg tgtacccata taaccaggag caatttcgta ttgagaaaaa
     8701 gtcaaaacta attgatgctc tttgttcaaa taaaaacttc gtgtaggcgt aacaagtgtt
     8761 ggtttttcta tcgtcccttc ttgaatatct tgctcagtcc aatagtactt agatggatct
     8821 tccttgactt gttcggccat ctgccgtttg acttcttttg tcaaaacaga tagataggtg
     8881 tcatctttga ataaaagtgg aagtgagagc tggacactgt ttttcttgtc gattgtgtcg
     8941 tatctttttt caacatggct atctgctgct gtttgggtga tctgcctttc aatcacgaga
     9001 aaccggttat cgtctacgat tttctgataa tctcccaaaa cttggtaatg atccgtttca
     9061 aaatttccat attctttttc agctttttca aaatctgctt gtccttcgcg aaagtatttt
     9121 tgatttaatc catgaattgt tttattttct ttctcatttt gtgtattgat cttcggtacc
     9181 tgaatatcga ttttttcaga tcgatcagta aagcgttcgc ctaagatcaa ttcaatgact
     9241 gatcctaaga tgggtaaatc ttctgcaaag cttcgaactt gactattaaa aagaaggcta
     9301 cttgttgtaa tcattacgat agccaaagat actccgaacc cttggagacg tctctttctc
     9361 cgctttgctt ttataaatga ttgctcttcg ataataaaac gctggcggat ctcctcttta
     9421 agccctttgg gaattgcttg ttctttataa gttttcttta gtttttttaa attttctttt
     9481 tgggaataca tggcactctt ctctccttca ttctaggatt tgacgtaaag cattcaatga
     9541 gcggtacatt ttcgttttaa ctgtgttttc attcatatcc aagatagtcg caatttcttt
     9601 caaacgaaat ccttcaaaaa agtagaggat cagaatctct ttttccggac tgtctatttt
     9661 atctaaaatc atatggagct ctgcaacgga agtttctaaa ggtaactgtt gggggatatc
     9721 ttgaatttca aataagtcca caggcgtcct tttttgtctg cgccaactat ctaatgccgt
     9781 gcggatcaat atccgataaa accatgcact aaaatgctcg atctcttttt ctttttcaaa
     9841 atagttcagg gctttatgaa aactatcttg catcacatct aaggcctctt ctttatcatg
     9901 tacgtaattc atcgtgatac ggaacatttt ttgataatca gtttcgatga gtccattcag
     9961 caggtgacgg tatttttcga tctgccgttt tttcttatat ccagtaaaaa tcattctcca
    10021 ctcctttctg cctcacttaa tatgacgtac aaggtgcgct aaaagtttca atttttttat
    10081 tttttatgta tttacttggg atttttatat acaaaaaaga aggtgtgaca aaaatcgtgt
    10141 cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta agaaaataat
    10201 gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc atcatcgtct
    10261 tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc atcatctgtc
    10321 gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc ttcatcttca
    10381 tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt atcttctggg
    10441 tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt gactttcttg
    10501 cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt tacttcttca
    10561 tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc taatgagata
    10621 aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg aatttcactg
    10681 tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc catgacatcg
    10741 ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct tttgtttgca
    10801 ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact ccttatcata
    10861 caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa atccaaggtc
    10921 aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat tcgaaccttc
    10981 cctgctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt agcaatctgg
    11041 atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc aaatttcaaa
    11101 cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac taatttgatc
    11161 gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat gtacagcgta
    11221 tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac ttccccttgc
    11281 tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa ttccattatt
    11341 cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt gtcaaccaac
    11401 ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta gagtataata
    11461 ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg attcgactag
    11521 atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat atattaacgg
    11581 aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac tgcacagtca
    11641 tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa acactggaag
    11701 atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta tcagatgaag
    11761 gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata accatagatg
    11821 acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca ccgatacaag
    11881 cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc catggcaaaa
    11941 aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg atgtacttaa
    12001 gtgtcaacgg taatcaggaa aaacgtgggc aagtcatgaa gcgattttat caatctgctt
    12061 taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca atggcaacac
    12121 ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca tttgcaaaag
    12181 cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg ctaaaaaaag
    12241 aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt aataacataa
    12301 aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga aaaagttttt
    12361 cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga tttgatcaat
    12421 tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc tttcactttt
    12481 cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat ttcaagacga
    12541 atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg aggctgggat
    12601 gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg tcatggtcct
    12661 tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac tgtagagatc
    12721 atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga aggttttcct
    12781 gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt ccaaatgatt
    12841 tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta tttcactgga
    12901 acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc ttatgaaggc
    12961 gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt tagccgatat
    13021 caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt aattttggag
    13081 catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt tgaactccat
    13141 tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata cctcaaactt
    13201 gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga tcctatttta
    13261 aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac tttttctggc
    13321 aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt cggatataat
    13381 cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt ttatcgccca
    13441 gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac tttgatagaa
    13501 ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg agatgagcga
    13561 tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga tttatttgac
    13621 gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga aataaattaa
    13681 aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga tggtacttta
    13741 ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc tgctgttgca
    13801 aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca ggaacagtta
    13861 aacgagttga acctttttca agataatgat tacgttatta cttataacgg ggcactcgta
    13921 caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga agatttccta
    13981 gagatcgaag taatggcacg ccgtgtcggc agtcatctcc attcgatcga tgacaaagcg
    14041 atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag tttagtcaag
    14101 atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat cgtcaaaatg
    14161 atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc gcaaactttc
    14221 cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact aaataaagaa
    14281 gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga tcaagatgaa
    14341 atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc tggtctagga
    14401 gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat cacttcatca
    14461 aacgacgagc acggtgtagc tgaagcaatc aaaaaatatg tactgtaata aataaaaaaa
    14521 ctgagagaaa gctgatctac tcgctttctt tcagttttta tttttttcga tccatctctc
    14581 gactgcccat cggtggctac cacgtttcaa tttcacaagc gcttcttgag ggctcaccca
    14641 ccaaatttta ttggtctttt cagttggttc tcctatacgc ttccaagaat cagcaacaaa
    14701 gaaatatcct ggattataat aataagttgc acgataattc gagtaaaaat actcatcagc
    14761 ttctcccaga taagaagaaa tttctgccac aattcccatt tcttctatca gctcacggac
    14821 aatcgcttct tctttcgtct cttctccttc aatttccccg ccaggaagaa aaaaagcacc
    14881 atttggagcc tcgattactg caatctgctg atctctttct atgacaatat atgctgcata
    14941 acgggtttga taatcagcaa attgcttttt ttcaccaaaa attggtttat ccatgaatag
    15001 tttccacctt aacagtttta ttttctatcc attttacatg agaaatttct catgaacaag
    15061 cacaaatcaa ctatttcctt acttcttttt gttatgattg agtaaaagga aaaggagaga
    15121 tgaatctatg aaaatggctc acacatgtgt tcgagtgaaa gatcttgaag catcaatcga
    15181 attttatcaa aaggcatttg gttttgaaga aagccgccgc cgtgattttc cagaaaacaa
    15241 atttacactt gtttatttga cacttcctgg ggatgactat gaattagagt tgacttacaa
    15301 ctatgatcat ggtgcgtatg atctaggaga cggctatggt catatcgcta tagcagctga
    15361 tgatctagaa aaattacacg agcaacataa agctgctggt cttgagatta ctgacttaaa
    15421 aggattacct ggcactgcgc cttcttacta ctttgtcatt gaccctgatg gttataaaat
    15481 cgaagtcatc cgaggatagt tagtatgggg cattttactc ttgattgaat aaaaaacaaa
    15541 caccaataat taaaatgaga cgcaaacttt tcattaaatt gaaaaagttt gcgtcttttt
    15601 ttaacaacca ttgtagctat aaggttgcca ctctccacca atttattttt aattcatttg
    15661 aaagaaatca aataaacgag gtacctttag aaaacaaaac tgcttttcct cctattatca
    15721 cacgatcttc ttttaattca cattctagat ccccgcccct tgatgacatt tgataagctg
    15781 acaattttgt tttattcatt ctttctgccc aataaggaat aagattagaa tgtgctgatc
    15841 cacatactgg atcttcattg attgttaatt ttggaaagaa ggttctagaa acaaagtctt
    15901 tttgcacact ttctgctgtc acgatgactc ctattcctaa ttcaaaattt ttgatagctg
    15961 taaaatctgg ctgcaattta gccaccgttt tttcatcttc taatacgaaa aataaatcac
    16021 gtgctaagta ggcttcttta atcttagccc caatcgcttc ttcatattca gacaatatcg
    16081 gcactttttt cggcataata ctaggaaaat ccatgtaata ataatcttta attcttgtca
    16141 caaatagatt accgctttga gtagaaaatt taatcgtttc atcaggtatt ttgtaatagt
    16201 taaataaaac aaatgctgtt gctaatgtcg cgtgaccaca aagatcgatt tctcgatctg
    16261 gcgtaaacca tctaagctca aattcttggt ttttcttcac tgtaaatgct gtttcagata
    16321 aattgttttc aatggcaatt ttttgcatcg taccttctgg cagccattct tctagcacat
    16381 aaaccgctgc tggatttcct ttgaacacct catctgcaaa tgcatcaact acgtaataat
    16441 ctaagttcat tttaaagccc ctttctctaa tttgttataa atatattctg cacaacaaag
    16501 atcttcaata gctagaccaa ctgcgtcgaa aatcgtcggc ctcgattctc ctttataaaa
    16561 tgtttttttc tcagaaacta attcgcctaa tgtaccaaca atacaagatt cctctaaatc
    16621 tccttctgcc atggctataa gcaggtcccc gctttctgtt aaggcagctg agtaatcatc
    16681 tacatagatt tgagaagttt ccatcaacga tgtacttatc tctcttgtat caggagtaaa
    16741 tgtgcccaca gcattgatat gtgcgtcttc ccttacccac tctttttgaa gaaatgcctc
    16801 tttactagag gtcaacgtac aaatgatttc cgctggacgt actgcatctt ctactgatat
    16861 tgcatttata aacgttattt taggatactg ttttttagct ttagcgataa aagtatttcg
    16921 tctttcttct aaaatatcgt aaacaaacac tgtagtgatg ggacgaatgg tagaaatcgc
    16981 agtcaaatgc gacaatgctt gttgaccagc tccaattaaa gcaagtgttt ctgcatgcgg
    17041 atttgctaag taatctgttg ccagcgcaga tacagcagct gttcgaatcc aggtgactgc
    17101 atttgcgttt atcaatgctt tgggtagtcc gttttgagca tcaaacaaga aaatctctcc
    17161 taaatgagaa ggtaaatgat actgatgatt atccggaaaa gcggtaatga cttttgtgcc
    17221 aaaatatctg ttctccccca aataagcagg catcatcgca aacatatttt tgccttttcc
    17281 gtcaggtaaa atttccacca atcgaggatt ttggctgatt ttaccacttt cataatcaaa
    17341 aaaacatttt ttcattactt gaatcgcttc ttcaaaacta aaagaagcta ctacttcctc
    17401 aaaattaata attttcatag acaattccct cctcacaaaa gattaacttc taaaatcaat
    17461 acaatcaatg gtaaaattgt atggtataca attttaagga gtggagaaaa tgcctattaa
    17521 ttcttatgac aattacccgt taacttggcg accagacaaa caaaaattga agcgtcctta
    17581 ttatcgatcg ttgattcaac aattagaaca agatatttta aagaataaat tacagaaaaa
    17641 cactcagtta ccctcccaaa gagagctagc tgattattta gatttaaatt ttactaccgt
    17701 tggcaaggct tataaatatg gaattgagaa aggtttgctg tatacgaata ttggaagtgg
    17761 cacatttatt tctcccaatg tctttgagtc catcaccatc tctaccaatg aagtaaccga
    17821 acatatcatt gattttggtc tggttagttc ttttgaagaa tgtaataaat tcgttgtccc
    17881 gtttattcaa tcaactagta ataacccaaa tcttgttggt ttgcttaatt accaagatcc
    17941 caaaggcaca aattttcaat tgactactgc agcaaattgg ttaaaaactc aaggagtcat
    18001 tccgtcctta gaatctctat ctattgtttc cggtgttcaa aatggattgg ctatcacact
    18061 ttctgcactt ttctctccag gtgatagaat agcaacagac cgatatacat acgctaattt
    18121 cattgaattg gctcagttat atcatttaga gatcgttcca attgattttg acgagcaagg
    18181 aatgcgacct gatatattga ttcaggaatg tagaaaaaag aagattcatg gtgtctttct
    18241 tatgccttct tgtaataatc ctatagggtt tcaaatctct cacaaaagac gagagcaatt
    18301 agccgaagtg attgaaaaag aaggtctatg ggttattgaa gatgatatcc attcattttt
    18361 aacaacttac tatcaacata cgatcattcc tcctttccaa aatttattac ctaaccaaac
    18421 aatctactta gctggaatga caaaatttat ttgttctgga ttgcgagttg cttatcttgt
    18481 agttccagta catgtccaaa gcaagataga acaagcaatt tttaatataa atgtgaaaac
    18541 ttctggatta gatgtggaag tcatcactca aatcctccat tcaaatgaag caaataggat
    18601 cttagaaact aaattaagtc ttacaaaaag agccaatcag ctttttgatt ctcttttcaa
    18661 agtagaaaaa ccaacgaatc gtgtccctta ctatcgaatt cttccagttg atcctgagat
    18721 tgaccctaag ataattgaga atgactttct tgctaacggt atccgacttt atcattccag
    18781 acgctttact acacgtgcgc aaccagatgc attcgttcga atttccctat cctctaataa
    18841 ccttgaaatt ttagcttctg gactaagtat cgtaaaagaa aaaataaaaa aatatgaatt
    18901 ataacctatt gataaataaa ggaatcaaat cttattgatt cctttcaata aaaaagaggc
    18961 actacccgag gttcaacaac gggtagtgcc tcaaaaaata aacgaacatt ttgcacttaa
    19021 agtatccaag aagcaagaaa aattatttta ttttcttatt ttactaaatc actaggtttc
    19081 tgtcagaaga tgaaattatc caatcatgac actttcttct ggatatttgt attttgattg
    19141 tgttctttgt gcttttgtca tcagagagaa gaccacagtc aatacaccaa ctcgtccaat
    19201 gtacataagc gctataatga tcaatttccc tattgcagtc aagtctggcg tcaatcccat
    19261 cgtaagacct actgtaccaa acgcagagaa gacctcaaaa gcaatatatt cgatgccaga
    19321 agttttaggt atcgtttcag tcaccgataa aatcatgatg gcgactacac acaacgtcaa
    19381 cgtaacaaaa aataaagtaa gtgcccgcaa cactgcgcct tgtcggatgg tacggccaaa
    19441 tgcttccgct ctcgtacgtc ctttcaacat cgaacgcatc tggatcagca acacacctaa
    19501 cgtagttgtt ttcaatccgc cggccgtcga cccagatgtt ccgccgatgt acatcaaaaa
    19561 catcgttaaa atcagacctg catggctcat atggaggtaa tcaactgaat aatatccagc
    19621 cgttctaggt gtcacactca taaaaaaggt gtttgccaat cgttgaaaca tattcccttc
    19681 cactagcgtt gccccattgc gctctgtgaa aaagaacaca acgaatccgc ctaataaaag
    19741 ggtacctgta acggttaatg cgatttttga atgaatcgtc atcttctttt tctgatgata
    19801 ttccagtaaa tcacgccata cgatgaagcc taacccacca gaaatgatca aagcagatac
    19861 caccatgatc aaataaacat tttgctggtg attggctaaa ctgtctccta acaaatcaaa
    19921 ccctgcatta caaaaactag agattgcatg gaacaaactg taccagatac ctttcaacca
    19981 gccaaattca ggcacaaata caaaagataa agcaaccgta ccaatcaatt ggatcacaaa
    20041 ggccaatttt aggatgtaaa gcatgagctt catcacacct gatacttctt ctagattcag
    20101 cgcttctttt aatataatac gtgtgctgag actgactttc ttctttatca atgcaaagca
    20161 tagaatcgga accatcataa aaccaagacc gccgatctca atcaaaagca tgatcaaaaa
    20221 ttgaccagca ctgttccaat gctctgctgt attcaatgtt gtcaatcccg ttacacagac
    20281 agcagaagtt gcagtgaaaa gcgcatcgat aaaatttgtc ccatgcccac tattactaaa
    20341 aaatggtagg gtaagtaggc cgccgccaac taaaatcagg agaaaaaatc ctaatgcgag
    20401 aagctgaaca gccggcaaac gtttctttat ttgtttgctt tgtttaatca tcgttttcct
    20461 cttttaaaaa aagataccac ttagtcagtg gtacccatat tttttacttt gatcaacctt
    20521 gttacttctg cgcgctcgtt ttcttccagc ttcatacgaa tataatagat cgtttcttct
    20581 aattgaggga tcgtcatata ttctaatgca ttgactcgcc gacgtgtctt ttcgatttca
    20641 ctcgccatca gttgacaagt tttttcgatt tctgtcagtt ccaaaagttt tggaagcagg
    20701 gtagtaaacc gatcgattga ccgatccagt tcggcactgg aattcaaata cccgtattcc
    20761 aaaggtgtgt ctgtcagctg ttcatcatat tcaaaattca tcacaggtac ttttacactc
    20821 atgatatttt tgtctacgat agacaaatca acgttttccg ccggcaaaac aaataattct
    20881 tcgatgaaag gttccgtgat cgaagcattt gccaatacaa aatcagacat tgcttctgtc
    20941 atagactctt ccatttcttg acgcagctgg ttgttctttc gaatcaacag gatgaattga
    21001 cgcatcagtt catcttgctt atcttttaat aatttatgcc ctcttgtggc agttgtcaac
    21061 tgttttttca atcgagtcaa ttccatgcgg gtcggattca cattcaatcg tgccatcaga
    21121 actcacttcc cttctggcag atatttgtcc agcatgtcgt ctttgattcg ttttaattcg
    21181 gttcttggca acatcgatag cagttcccac cctagatcaa gtgtttcagt aatcgaacgg
    21241 ttcgtgtaga atccttgatt gacatattct ttttcaaacc gctcagcaaa cttcgcatag
    21301 atcttgtcta catcagataa agcagattca cctaaaacta cggctaattc ttttgcctgt
    21361 tttccttgtg cataggctgc aaaaagctga ttcatcgtag ctgcatggtc ttctcttgtc
    21421 tttccgggtc cagttccctt atcttttaac cgcgaaagag acgggagtac atcaattggt
    21481 ggctgaatcc cactcttgta aagctctcta gtcaagatga tctgtccttc tgtaatataa
    21541 ccagtcaagt cagggattgg atgagtttta tcgtcttctg gcattgttag aatcggaatt
    21601 tgagtaaccg atccttttag tccgcggatt cttcctgctc gttcgaataa agtagctaag
    21661 ttcgtgtaaa gatacccagg atatcctctt cttcctggaa cttctcgccg tgctgcagaa
    21721 atctctcgca atgcctctgc atagttggtc atgtctgtca tgataaccaa aacatgcatt
    21781 cccttttcat aagcaagata ttctgcagca gtcaaagcca tacgaggagt agcgatacgt
    21841 tcgattgcag ggtcgtttgc taaattcata aacatgacag aacggtcgat tgcgcccgtt
    21901 tgacgaaaat cttccatgaa aaattcagct tcttcaaaag tgattccaat agcagcaaag
    21961 actacggcga aatcatctga tgaatcgagt accgttgctt gtcgcgcgat ctgagcagct
    22021 aattctttat gaggaagacc agagccagaa aatactggta atttttgccc acgaactaat
    22081 gtattcaaat gatcgattgc agagatacct gtttggataa actcatctgg ataatcccga
    22141 gctactggat tgattacttc tccgttaata tccaaatgct tttcaggcag gatctctggt
    22201 ccgttatctt tcggtcgtcc caaaccatcg aatactcgac cgatcatgtc ttccgataca
    22261 cctagttcta aaggatggcc taaaaatcgt acagaagagt cacgtaaatt gataccactt
    22321 gttccttcga atatttggac cattgcttta tcttcttgga cttccaatac ttgtccacga
    22381 cgaatctcgc cattttgcat acggacttcg attaattctt catatttgac accagcaact
    22441 ttttcaaccg ccatcaatgg accgacgact tcaccgatcg tacgatattc tttaatcatt
    22501 gctcatgcct ccttctgaaa cgatcgtttg catcgtttgc ttgatttcac caataatttg
    22561 atctagtttt tctaattctg tttccgggat atatttgctt cgtccgatcc tttcacgaat
    22621 cgatacggta ccgtccatga tctcacttaa ataagcacct aacgacaacg cacggcgtcc
    22681 ttcttcacca aaggtcaaga tcatcttcaa cattttgaat tgtttctctc ttgaagtgaa
    22741 tgtatcgaca tcatcaaaag cattttgctg caaataatct tcacgaatgg attttgccac
    22801 ttctaatgtc agacggtcat tatctgaaag ggaatcaatc cctaccaaac gcacgatttc
    22861 attcagctgc tgttcttcct gcaaaatacg catgccctcg gtgaccatgg atgcccagtc
    22921 agcttgaagg atatgatcca tatacttccc tacttctgtt gaataaagag aatagctttg
    22981 tatccaatcg atcgaaggaa aatgtctttt ttgggctaaa ctggaatcaa gaccccagaa
    23041 tacttttacg acccgcaatg tattttgtgt gactggttcc gaaatatccc ctccagacgg
    23101 ggatacagca ctgattgcgg ttatacttcc ttcgcgacct tctgatccta atgcaatgac
    23161 tcttccagat cgttcatagt attccgcaag ccgtgaacca agataagctg gataaccttc
    23221 atcaccaggc atttcttcca aacgtccgct catctcacga agtgcttctg cccatcgtga
    23281 agtggagtct gccatgatag caacgtcata acccatgtca cggaaatatt ccgcaattgt
    23341 gattcccgta tagatagaag cttctcgagc tgctactggc atgtttgatg tattagcaat
    23401 caataccgta cgttccatca acgattctcc tgtattaggg tcaatcaatt cagggaattc
    23461 atttaaaaca tccgtcatct catttccacg ctcgccacat cccacataga caaccatgtc
    23521 cacatcggcc cattttgcaa tttgatgctg aacgactgtt ttccctgcac caaaaggacc
    23581 aggtacagct gctgctcctc cttttgtcac tgggaagaat gtatcaatca ctcgctgtcc
    23641 agtgatcatg ggtgcatccg ggtttaattt ttctttgatt ggccgactac gtcttactgg
    23701 ccatttttga agcatcgtca aatttttgtc accattttcg gtttcaatga cacatactgt
    23761 ttcatcaata gtgaatgaac ctttttcgat tgttttgatc gttcctttta tcccatttgg
    23821 caccatgatt ttatgtttta taacttttgt ttcttctacg atcccgataa catctccagc
    23881 actcaccgtt tctccggctt ctactgttgg ttcgaaccac cattttttct catggtccaa
    23941 tgcgggcaat tgaactccgc gccctaagaa attactttgt gtgatttcca taaaggtatc
    24001 taatggccgt tggattccgt caaacatttg tgaaatgatc cctggtccta attcaacaga
    24061 taaagcttca ccagtagagc gaaccggttc gcctggacca attccagaag tctcttcata
    24121 tacttgaata gacgccacat cgccacgcat ctcgataatt tctccgataa ctcctagatc
    24181 tcccactaga cacatgtctt ggatactggc atcagacatg ttctcagcca ttacaagagg
    24241 tccggagact tttatgattt ttccaatttg caaaatttct atctcctttt catactataa
    24301 gatattttga cctactgctt tttctacgtt tttttgaatt tcttccagcc ctatccctaa
    24361 tgatccttga tgattgggaa tcaatacgat agcaggtatc atttgttcct tgtaacgttc
    24421 gatcgtttct gggctcaatg ctgcacattg ctctgtgaca taaatcacgc catactcatt
    24481 ctttgccatt tcttcaattg cctgacgaac ggtttgcttg gttgtaccat aacgaacatc
    24541 aaagccaaac aatttgaatg gcaaaacaga atctttgtcg ccaactacac cgatcttatg
    24601 cgtcatagac ttgtctcacc cgctttctga ttgtttctga ttcgaaatgg cttcttttgc
    24661 cgataaccag taaacgaagg tttttgcttt ctacctcttt tgcgttcaaa aaggctagta
    24721 gtggcagtgg tccgaaagcc tgggtttgtg cttcttgata caatgaactt agataatcat
    24781 ctttcaactg ttcaagcttt acgagatcaa tatttccctc acggatgaca gggcgcagaa
    24841 aatcagcata atctgtctct aatagaaaat tcgtgtaatc ttccatatct ttatctacaa
    24901 agttcaaaaa tacttctttt tcgatacttc ccgagctgga aagtaccgtc gtcatgaacc
    24961 catatgttct tttctgcaat attcctcgag ccatcgttgt gatattggtc aagtcaataa
    25021 aagcaatgac ttctgtcagc aattcttgat atcctagctt atcggcaagc cgtcgctgct
    25081 ctgtcaaaaa ctgtcgatca taaatcacgt caattccctg aagaatggta gactcttcaa
    25141 aataatcacg tacttctcgt atactgtcca taatgctttc aggaagttca tttgaaactt
    25201 gcgtatgaat ggcatccttc aaggtttcta atgaataaaa tccatcgtcg atatacaaat
    25261 ggtccagatt ttgatttgtc atctcagctt ttgtcaaaac cttcagatta tggaaagtat
    25321 aacgcatcgt atagatccaa acgatttcag gctccggtgc tgattccttt aaccagcgaa
    25381 atagacggct ctgctccttt aaaagattct gttcgaagtc atattcatat ccgtcataaa
    25441 tatatggact gtaaatcgtg gatttcaata tttcaccaag tgcttcgaca gaatttgttt
    25501 gaatcatccg ttcaaatgtc tcattggaca aaagttcaag ctctcttccg cgaatcaagg
    25561 gatttagttc gtggtattcc atacactgat ccctccttat tcgaataatt gattggcaat
    25621 ctcaaaactc atcgttcctt ggatatcttg tacaaggctg ctgaaaagaa aattatactg
    25681 aactccttga tcgtttatca gaaaacccgc ttgatcagca acagtttcat cacttaaaac
    25741 catcataaag ggaagttcat tgttccattc atccaacaac gtctgagaaa gatagg
//
LOCUS       DASJON010000017        24653 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJON010000017 DASJON010000000
VERSION     DASJON010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.017"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="U3989_000541"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="U3989_000541"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1714010.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="U3989_000542"
     CDS             complement(860..1678)
                     /locus_tag="U3989_000542"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1714011.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="U3989_000543"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="U3989_000543"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN1714012.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="U3989_000544"
     CDS             complement(2967..3923)
                     /locus_tag="U3989_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714013.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="U3989_000545"
     CDS             complement(3923..5056)
                     /locus_tag="U3989_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714014.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="U3989_000546"
     CDS             complement(5049..6611)
                     /locus_tag="U3989_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714015.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="U3989_000547"
     CDS             complement(6823..7905)
                     /locus_tag="U3989_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1714016.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="U3989_000548"
     CDS             complement(7971..8369)
                     /locus_tag="U3989_000548"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN1714017.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="U3989_000549"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="U3989_000549"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN1714018.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="U3989_000550"
     CDS             complement(9098..10399)
                     /locus_tag="U3989_000550"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN1714019.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="U3989_000551"
     CDS             complement(10540..11556)
                     /locus_tag="U3989_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1714020.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="U3989_000552"
     CDS             complement(11864..12469)
                     /locus_tag="U3989_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1714021.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="U3989_000553"
     CDS             12665..13588
                     /locus_tag="U3989_000553"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN1714022.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="U3989_000554"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="U3989_000554"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1714023.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="U3989_000555"
     CDS             complement(15703..17334)
                     /locus_tag="U3989_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN1714024.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="U3989_000556"
     CDS             complement(17348..19021)
                     /locus_tag="U3989_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN1714025.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="U3989_000557"
     CDS             complement(19034..19351)
                     /locus_tag="U3989_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1714026.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="U3989_000558"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="U3989_000558"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1714027.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="U3989_000559"
     CDS             21013..21798
                     /locus_tag="U3989_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1714028.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="U3989_000560"
     CDS             21801..22493
                     /locus_tag="U3989_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1714029.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="U3989_000561"
     CDS             complement(22767..23138)
                     /locus_tag="U3989_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN1714030.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="U3989_000562"
     CDS             complement(23538..23768)
                     /locus_tag="U3989_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714031.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="U3989_000563"
     CDS             complement(23862..24200)
                     /locus_tag="U3989_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714032.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT         7159 a         5463 c         4354 g         7677 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aat
//
LOCUS       DASJON010000018        24551 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJON010000018 DASJON010000000
VERSION     DASJON010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.018"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U3989_000564"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U3989_000564"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1714033.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="U3989_000565"
     CDS             complement(937..1518)
                     /locus_tag="U3989_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1714034.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U3989_000566"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U3989_000566"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN1714035.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="U3989_000567"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="U3989_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN1714036.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="U3989_000568"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="U3989_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN1714037.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="U3989_000569"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="U3989_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1714038.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="U3989_000570"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="U3989_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1714039.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="U3989_000571"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="U3989_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN1714040.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="U3989_000572"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="U3989_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN1714041.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="U3989_000573"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="U3989_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN1714042.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="U3989_000574"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="U3989_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN1714043.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="U3989_000575"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="U3989_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN1714044.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="U3989_000576"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="U3989_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN1714045.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="U3989_000577"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="U3989_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN1714046.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="U3989_000578"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="U3989_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN1714047.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="U3989_000579"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="U3989_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN1714048.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="U3989_000580"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="U3989_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN1714049.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="U3989_000581"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="U3989_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN1714050.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="U3989_000582"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="U3989_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN1714051.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="U3989_000583"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="U3989_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN1714052.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="U3989_000584"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="U3989_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN1714053.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="U3989_000585"
     CDS             13972..14157
                     /locus_tag="U3989_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN1714054.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="U3989_000586"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="U3989_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN1714055.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="U3989_000587"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="U3989_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN1714056.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="U3989_000588"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="U3989_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN1714057.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="U3989_000589"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="U3989_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN1714058.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="U3989_000590"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="U3989_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN1714059.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="U3989_000591"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="U3989_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN1714060.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="U3989_000592"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="U3989_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN1714061.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="U3989_000593"
     CDS             18232..18879
                     /locus_tag="U3989_000593"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN1714062.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="U3989_000594"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="U3989_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN1714063.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="U3989_000595"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="U3989_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN1714064.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="U3989_000596"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="U3989_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN1714065.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="U3989_000597"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="U3989_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN1714066.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="U3989_000598"
     CDS             20317..21255
                     /locus_tag="U3989_000598"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN1714067.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="U3989_000599"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="U3989_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN1714068.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="U3989_000600"
     CDS             21935..22774
                     /locus_tag="U3989_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1714069.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="U3989_000601"
     CDS             22750..23619
                     /locus_tag="U3989_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1714070.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="U3989_000602"
     CDS             23609..24406
                     /locus_tag="U3989_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1714071.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT         7953 a         4548 c         5279 g         6771 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa a
//
LOCUS       DASJON010000019        24337 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJON010000019 DASJON010000000
VERSION     DASJON010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.019"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..2477)
                     /locus_tag="U3989_000603"
     CDS             complement(132..2477)
                     /locus_tag="U3989_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1714072.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="U3989_000604"
     CDS             2756..3658
                     /locus_tag="U3989_000604"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN1714073.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="U3989_000605"
     CDS             3869..5644
                     /locus_tag="U3989_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN1714074.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="U3989_000606"
     CDS             complement(5710..7296)
                     /locus_tag="U3989_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN1714075.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="U3989_000607"
     CDS             7547..7771
                     /locus_tag="U3989_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN1714076.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="U3989_000608"
     CDS             7913..9664
                     /locus_tag="U3989_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1714077.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="U3989_000609"
     CDS             9664..11448
                     /locus_tag="U3989_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714078.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /locus_tag="U3989_000610"
                     /pseudo
     CDS             11783..12635
                     /locus_tag="U3989_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12732..14618
                     /locus_tag="U3989_000611"
     CDS             12732..14618
                     /locus_tag="U3989_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1714079.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="U3989_000612"
     CDS             complement(14659..15561)
                     /locus_tag="U3989_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1714080.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="U3989_000613"
     CDS             15791..17194
                     /locus_tag="U3989_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1714081.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17191..18627
                     /locus_tag="U3989_000614"
     CDS             17191..18627
                     /locus_tag="U3989_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1714082.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="U3989_000615"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="U3989_000615"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN1714083.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="U3989_000616"
     CDS             19911..21638
                     /locus_tag="U3989_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN1714084.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21654..22004
                     /locus_tag="U3989_000617"
     CDS             21654..22004
                     /locus_tag="U3989_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1714085.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="U3989_000618"
     CDS             complement(22098..22958)
                     /locus_tag="U3989_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN1714086.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="U3989_000619"
     CDS             23050..23478
                     /locus_tag="U3989_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1714087.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /locus_tag="U3989_000620"
     CDS             23640..23816
                     /locus_tag="U3989_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN1714088.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="U3989_000621"
     CDS             23843..24289
                     /locus_tag="U3989_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN1714089.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5186 g         7090 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaattc
//
LOCUS       DASJON010000020        23829 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJON010000020 DASJON010000000
VERSION     DASJON010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.020"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..789)
                     /locus_tag="U3989_000622"
     CDS             complement(55..789)
                     /locus_tag="U3989_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1714090.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="U3989_000623"
     CDS             1116..1526
                     /locus_tag="U3989_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN1714091.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="U3989_000624"
     CDS             1519..2220
                     /locus_tag="U3989_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN1714092.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="U3989_000625"
     CDS             2220..3806
                     /locus_tag="U3989_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1714093.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="U3989_000626"
     CDS             3825..4046
                     /locus_tag="U3989_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN1714094.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="U3989_000627"
     CDS             4043..4804
                     /locus_tag="U3989_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN1714095.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="U3989_000628"
     CDS             5184..5693
                     /locus_tag="U3989_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1714096.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="U3989_000629"
     CDS             complement(5768..6307)
                     /locus_tag="U3989_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN1714097.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="U3989_000630"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="U3989_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN1714098.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="U3989_000631"
     CDS             7320..8171
                     /locus_tag="U3989_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN1714099.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="U3989_000632"
     CDS             8199..8834
                     /locus_tag="U3989_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN1714100.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="U3989_000633"
     CDS             8854..9627
                     /locus_tag="U3989_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1714101.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="U3989_000634"
     CDS             10035..10832
                     /locus_tag="U3989_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN1714102.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="U3989_000635"
     CDS             10810..11223
                     /locus_tag="U3989_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1714103.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="U3989_000636"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="U3989_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1714104.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="U3989_000637"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="U3989_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN1714105.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="U3989_000638"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="U3989_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN1714106.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="U3989_000639"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="U3989_000639"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN1714107.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="U3989_000640"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="U3989_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN1714108.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="U3989_000641"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="U3989_000641"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN1714109.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="U3989_000642"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="U3989_000642"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN1714110.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="U3989_000643"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="U3989_000643"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN1714111.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="U3989_000644"
     CDS             21907..22485
                     /locus_tag="U3989_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1714112.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="U3989_000645"
     CDS             22537..23019
                     /locus_tag="U3989_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN1714113.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="U3989_000646"
     CDS             23136..23510
                     /locus_tag="U3989_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714114.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4286 c         5117 g         6644 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagtt
//
LOCUS       DASJON010000021        23724 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJON010000021 DASJON010000000
VERSION     DASJON010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.021"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..613
                     /locus_tag="U3989_000647"
     CDS             <1..613
                     /locus_tag="U3989_000647"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1714115.1"
                     /translation="FQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFDTEPLSHGN
                     KGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTS
                     GTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSN
                     HFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(806..1045)
                     /locus_tag="U3989_000648"
     CDS             complement(806..1045)
                     /locus_tag="U3989_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1714116.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(1069..2592)
                     /locus_tag="U3989_000649"
     CDS             complement(1069..2592)
                     /locus_tag="U3989_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1714117.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(2610..2732)
                     /locus_tag="U3989_000650"
     CDS             complement(2610..2732)
                     /locus_tag="U3989_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1714118.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(2762..3745)
                     /locus_tag="U3989_000651"
     CDS             complement(2762..3745)
                     /locus_tag="U3989_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1714119.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(3769..3918)
                     /gene="rpmG"
                     /locus_tag="U3989_000652"
     CDS             complement(3769..3918)
                     /gene="rpmG"
                     /locus_tag="U3989_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1714120.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(3939..4316)
                     /locus_tag="U3989_000653"
     CDS             complement(3939..4316)
                     /locus_tag="U3989_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714121.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(4348..4527)
                     /gene="rpmF"
                     /locus_tag="U3989_000654"
     CDS             complement(4348..4527)
                     /gene="rpmF"
                     /locus_tag="U3989_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1714122.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(4542..4811)
                     /gene="rpsN"
                     /locus_tag="U3989_000655"
     CDS             complement(4542..4811)
                     /gene="rpsN"
                     /locus_tag="U3989_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1714123.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(5334..7100)
                     /locus_tag="U3989_000656"
     CDS             complement(5334..7100)
                     /locus_tag="U3989_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714124.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(7060..8814)
                     /locus_tag="U3989_000657"
     CDS             complement(7060..8814)
                     /locus_tag="U3989_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714125.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(8924..9493)
                     /locus_tag="U3989_000658"
     CDS             complement(8924..9493)
                     /locus_tag="U3989_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN1714126.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(9511..10926)
                     /locus_tag="U3989_000659"
     CDS             complement(9511..10926)
                     /locus_tag="U3989_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1714127.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(10937..11605)
                     /locus_tag="U3989_000660"
     CDS             complement(10937..11605)
                     /locus_tag="U3989_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1714128.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(11736..12320)
                     /locus_tag="U3989_000661"
     CDS             complement(11736..12320)
                     /locus_tag="U3989_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1714129.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(12655..12885)
                     /locus_tag="U3989_000662"
     CDS             complement(12655..12885)
                     /locus_tag="U3989_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN1714130.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(12900..13847)
                     /locus_tag="U3989_000663"
     CDS             complement(12900..13847)
                     /locus_tag="U3989_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1714131.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(13847..14689)
                     /locus_tag="U3989_000664"
     CDS             complement(13847..14689)
                     /locus_tag="U3989_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1714132.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(14822..15547)
                     /locus_tag="U3989_000665"
     CDS             complement(14822..15547)
                     /locus_tag="U3989_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1714133.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(16008..16322)
                     /locus_tag="U3989_000666"
     CDS             complement(16008..16322)
                     /locus_tag="U3989_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1714134.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(16338..17933)
                     /locus_tag="U3989_000667"
     CDS             complement(16338..17933)
                     /locus_tag="U3989_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HEN1714135.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(17956..18846)
                     /gene="iolE"
                     /locus_tag="U3989_000668"
     CDS             complement(17956..18846)
                     /gene="iolE"
                     /locus_tag="U3989_000668"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEN1714136.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(18863..19888)
                     /gene="iolG"
                     /locus_tag="U3989_000669"
     CDS             complement(18863..19888)
                     /gene="iolG"
                     /locus_tag="U3989_000669"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEN1714137.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(19912..20919)
                     /locus_tag="U3989_000670"
     CDS             complement(19912..20919)
                     /locus_tag="U3989_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1714138.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(21032..22192)
                     /locus_tag="U3989_000671"
     CDS             complement(21032..22192)
                     /locus_tag="U3989_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEN1714139.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(22268..23722)
                     /locus_tag="U3989_000672"
     CDS             complement(22268..23722)
                     /locus_tag="U3989_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEN1714140.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         7246 a         4876 c         3667 g         7935 t
ORIGIN      
        1 ttttcagtgg tcgccagaga aaatctcgaa tcgtttgaag catgaacata tggacttttc
       61 aattagtttt atcacgatct accgtgcaat ttgcaatgat cttttcgata cagaacccct
      121 ctcacacggc aataaaggcc tgatccgttc attgcaccat cgaggaaaaa caagacatac
      181 aaaaaatcat gtcgagtctc gtgggaagat ttgccttact cattcgatcc atggccatcc
      241 aaaatccgca gatgatagaa gtgattttgg tcattggaaa gccgatattg tagctggcaa
      301 aactagtggt acttgtttag tgactttgac agacagaaaa tcatgcttcc ttttagcagg
      361 aaagaaggtc cccagaaaga aagccgctta tgtgagcgag aaaatgattg aacttttaag
      421 cagtattcca aatgaggcgg tcaaaacgat cactccagat agaggaaaag aattctccaa
      481 tcatttcgca gttacagaag tattgaataa tatgcccttt tattttccag atcctcatgc
      541 ccttggcaac aatgaacaaa ttaaaatacg aatagactca ttcgagaata cttgccaaaa
      601 tcgaaagaga tagctacggt agataactca acgataaatc agatgattga aaaactgaat
      661 aacagaccta gaaagtgttt agattggaga acaccttatg aagtcttctt tgataaagtg
      721 ttgcgcttga tttgacaatt caagaaataa taaaaagcag atcaaatcca aataagggaa
      781 aaatgatcta ctctggtatt atcaattaat tggccaagct attatgccgt tttgctaaac
      841 gtctacttct tccagtattg accttgataa aacaacaatc aggatcccgt tttaatactc
      901 tatgaactgt gtcaatacta acatgtaaag ttgcagcaat atcagctaat gtactcccat
      961 ttaatctcat ttgctttgca aattgataac gccgataagc atcaatattc ttggaatcca
     1021 tagacaataa tacttttctt accatgtatt tcctccaaag attggatttt aatgggccat
     1081 catttcttga gcaatctcat gctcagataa tgaagctgga tagaaagtag gccaattatg
     1141 catttcatta taaagttttt cttgactttc tccaccaaag aaaatatgga aatgctctgc
     1201 cttacttggt gcaattccat gatcgctaaa ttgaatatat ttaaaacgac cagcacctgc
     1261 atcatcagtt tcaaaattaa aacgaacacc acggtttcct ttttcataat tcaaaatttt
     1321 gtatccaaca tatttgtatg tgtattgatg atgttcaccg ttgactacaa aatcaatggt
     1381 gttgtcttta atgttgatag tatcaatatc tgtcttatag ccagtagtat aataatcttt
     1441 gtactcggca gctgtcatgt ctttattgag ttttgctttg taatcgaaaa cttcatccaa
     1501 aattccatct tttaacaacg gataaacaga ttgccattcg cctgtataat cactcaattc
     1561 gcgatccttc acatcggcat ctttaaagta cccattagca actgtctttt catccttcgc
     1621 ttctttttct ggaactacct cttcaccagc aaccatagtt gttttctcta acgcttttag
     1681 attgtcttcc attactgaaa cataatcttc accattatcc atttgttctt ttgtaagact
     1741 ttcaagagga ttcaatactt ccaattttat cccagcttca ttggccaaag tttttgcgat
     1801 tttatcatta gcattctttt caaagtaaat ataattgatc ttattttttt taacatactc
     1861 ttttaattca gctaatcgac cagaagatgg ctcttcttca ggacttagtc cagcaatagg
     1921 aacttgtatc aatccataat ccaaagctaa atatccaaag gctgcatgtt gtgtcacaaa
     1981 acttttttgt ttcgcttctt taagactagt ggtatagtca gcatccaacc gtttcagttt
     2041 agtcaaatac ttttcagcat ttgtctcaaa tacttttgct tttttcggat acaatttaac
     2101 taactgatcc ttgatatttg aaacttcctt aattgccatt ttgggtgaaa cccacgtatg
     2161 aggatctagt tcatgatggt gatcttcttc tccgtgatca tgatcttctt cttcactacc
     2221 tggcaataaa atcatgtctt ttgttccttc tactacatta ggcccagctt tcttccaact
     2281 ttctttagct tttggtaccc atgattccat gttttcgtta tgatatacaa aaacatcagc
     2341 atcatgagct tttgccatat ccttagctga gggctcatag tcatgaggtt ctgatcccgc
     2401 tggaaccatt aaatccacgt tcgcttcatc gccgacgatg tttttggtga aatcatacat
     2461 tggatagaat gttgttacaa tttctaattt tccatcttta tcattcgata cttttgcatc
     2521 attagtgctg gagcaagctc ccaataatcc tagagctgct atcccaataa gtcctacact
     2581 gattttcttc attctttttc cttccttaat tatgctaatt tcttcatttt attttgttta
     2641 tgttctttga taatttttaa tgcccttttt ctagttttga tattcgggct tttaagtcgt
     2701 cgatatgcac tagatatatc cgttttctcc attagaaatt acccctaatc aatacttatc
     2761 attatttgct ttccgccagt aatttatcaa aactctgttt gatcctttct tcatccaaca
     2821 tttttccaat tagaataata gtggtttggt ctttttgtcg agtggtgtat tgaaaattaa
     2881 cctgttcgtt taccccttgt atggcaacta taaaatcttg atcttgcaaa gcaatcaatc
     2941 ctttaaaacg atacaggctc ccttggttgg ttaaaatcag ccggtcaatt aattggaata
     3001 aaaaatcttt ttttatgtct tgatctgaag ttaagagaat tgatttaaac tcatgttctt
     3061 gatgatgttg atgatagtgt ttgccattat agtgattttc tgatgaatca ctttcaaaaa
     3121 ctaagggatg attgaatttt tccaaattat aaaaatcctt tttttgaatc gtttcactat
     3181 gtgtgaacat tcgaatatcg gataaagggt taatactttt gatttctctc tgtaattttt
     3241 ttagtgcaga taaattcaag ttttgttttc tagacacaaa aatgcgatct gctatgacaa
     3301 gttgcttaag ggcttgtggt tctctttcaa taagttcaaa actgtcacta tcaataacag
     3361 tcaataaact atcgatatat actcttcctc ttagttgagg cgctgttaaa agtgtttgaa
     3421 tgattggttg gggatctgct atacctgtag tttcaatgat gatttgtgaa attggttgtt
     3481 gatgttcttc tattgtttcc agtaaagaaa tcaatattgc taataaatca ctgcgaagac
     3541 tgcaacacat acatccatta tttagttgta acacccgttc ctcgctatga actaataatt
     3601 tgtgatcaat cccaacttca ccaaactcat tttcaattat aataatttct tccctggggt
     3661 agggtgagac ttgcaaggct tgattgatta gcgttgtttt tccagcccct aaaaacccag
     3721 aaacgattgt aactggtatg cccattttgt caccttttta ccacttagct atttcacttc
     3781 tttgaaaatt gcccgtctgc gcaattttgg cgaatacttt tttaattcta accgttcagg
     3841 gttattccgt ttatttttgc tggtgagata cagacgttct ccagtttcga cacattccaa
     3901 aataatattt tgacgcataa atcttcctcc ttcaatactt atcgatcttc aagatatgct
     3961 tgaatttgtt gtgcatcttc aaaagttaac tcgccagatt ccaattgtga aactaaattt
     4021 tctttcatat caacaggtat ctcccaataa ccatcaatac attgaaataa ctcctcgtca
     4081 tactcttcaa aaattggata gattcttaaa aaatcatcaa atgataagcc ttggtcctca
     4141 tagtaatctt tgactaatcc attttcaatt gcctgttggg tcggtaaacc cgtttcagta
     4201 ataattcctc tctctaccat cgaacgataa aaagtgcatt tatctagcga ttgctgggtc
     4261 gtttcaagat aatactttaa tccttcttca actaaaggtg ggtaaataat ttccattttg
     4321 attccctcct aaaagttata tattaaacta ttttgaagct cctttaatta ctttctttcc
     4381 attatagtac ccttttaaag atacatgatg gctacgtcta taatcaccat tcgcttcgtc
     4441 aaatgaaatt tctggtttta ctacagtttg atgagttctt cttaaccgtt tcttcgcctt
     4501 agatgttttt cttgctggta ctgccatgat atttcctcct tttaccaact tgcttttttt
     4561 actccaggaa tcaacccttg atgggcaagt tctctaaact taatacggga cataccgaac
     4621 tttcgcatat accctcttgg acgaccatcc gtctgatcac gaagcctcag acgattcgga
     4681 tttgaatctc ttggcaattt agcgagagcg gtgcgatctc ctgcggcttt taattcctga
     4741 cgtttatctg cgtacttttc aatcatcgcc atttgtttct ttgctttagc aattttcgac
     4801 ttttttgcca tataccttta tactcctttt attttgattt agacatctca aatcgtaatc
     4861 gttacgtttt gacactgata ctttaccaac aagagttgct cttgtcaaca ataaataaca
     4921 tgaaataaaa ctcatatgaa tagataacta tttgaaaacg aatttatcgg tacatagaga
     4981 taatcaaatc atttaaaaga atatgcagtt taagctctga aaaataagaa ttcactttta
     5041 ttagtccaat caaacacaaa taccgtttta agtagctttt cctctactta aaacggcaat
     5101 atgaaagaaa ctataacttt ttattcaatc ttttatgttt aactggttta tcgccaattt
     5161 tttaaaactt tgattattcc ttatattata agaatagaca ttaagtgaac taattcaatc
     5221 atactgattc tttcgtgtat tcaaaattag atgctgttta ataaaattat tctttttgaa
     5281 aaagaattat aactatatcc caaaaattgt aggttaattt aaagcctatt tcatcatcgt
     5341 gattgagctt ctttttcata gacgaatttc tcagatagtt tagttttctt tatgagattt
     5401 ttgtatattt ctgaccttcg atacaactca ctatgagtac cttgatcaac tatttttcct
     5461 tgatccatca ctacaatttt atttaccttt tcaatagact tcaagcgatg cgatataatt
     5521 agtactgtct tatcttgtat taaacgattt aaactttcct gaatacgttt ttcattttcc
     5581 gcatctaaac tggcagtaat ttcatctaaa attaaaattg gcgcattttt caaaaaagcc
     5641 cgtgctatgg ataggcgttg tcgctctcct ccagaaagtg aacttccgtt ttctccgacc
     5701 aatgtatgat aaccgtcagg tagtttttca ataaagtctt cgcagttagc taaacgagct
     5761 gcttttttca cttcctcatc tgacgcttga ctatttccaa tacgtatatt ttccaaaata
     5821 cttgtattaa acaaagttac ctcttgaaaa acaatagcga ttttgctaaa caaagaagct
     5881 ggagatacac gtttaatatc atacccatca attaaaatac agccttcatc ataatcatac
     5941 agtctagaca ctaatttcaa gatactagtt ttcccggaac cactagcacc tactaacgca
     6001 gtcacttctc cttgtttagc cgtaaaagag atatgatcta aaattggggt attgttatca
     6061 tatgagaaag agacatctcg caactcaatg tcaaaagatt tcagaggact atctgatccc
     6121 tcttggatac tggtttcctt catagctcta attcgctgta ttttaggagc taaataaaat
     6181 atttcaagca ctgcttcttt catagaatcg aaggaatcct ttatttttat tgcggctagt
     6241 aagtaaccaa cgacgtagag tatagtcact tcacccgtta acagtaagtg aacacctact
     6301 agaatgacta ctgctaaact aacatatgag aaaatagagg ataaggccat gactgaaaaa
     6361 gtacttaatt ctacctttaa atgaatcctt tcactctctt ccattttttt gaaaagtctg
     6421 tcttgaactt ttttagatag attaaagcta ttaatctctt gctgcaattc tatagtttct
     6481 tgaaattctt ccgagttttc tctcaaagta tcgtaatatt tagtattggc tttcgtttgg
     6541 gatttctttg ataacaagat taagacaaaa ctaaataacg ttggcaaaat aacagctaat
     6601 cccattttga cattaccaac caaaagcatc actgaaataa aagggaaaaa cagagccata
     6661 ccgccggctt taggtattgc atgactcatc gcatgctcaa taccttcaac gtcagacatg
     6721 atagtttgag ataaatctga taaattatgt tttgaaaaat aggataatgg taaatttgac
     6781 aaatcgtctg caatttgcat tcttaaatgc gcactttctt tataagtact attatataag
     6841 ttttcatact ccctatctaa caaccaatac atgaaaagca aagtaacgaa cgatactgta
     6901 aaataaagcc aaggcggctt tacattttct aaaactaatt cgtctatcag catcattata
     6961 atgaatgctg gtaccatatt aatgcagtat acaaaaaagc tagaaataga tgctttaacc
     7021 aaatcttctg cgccctgatt agttaaagcg aatttgtttt tataaaaact tttcattgtc
     7081 aagcctccaa tcatttgcat tctcataatt agtgaccaat tgtttataca cttgatgttt
     7141 agccattaat tcattatgat ttccacgttc tactattttc ccttctgaca agacaagaat
     7201 ttcatctaca gatttaatgc tggataatcg atgagctatc ataataactg ttttttcttt
     7261 catcagatgt tgaaaagcct tttgtagttc atactcatta tctggatcaa tagatgcact
     7321 ggcttcatcc ataataacaa tctttgcatc ttttaaaatt gctctggcaa tagctatacg
     7381 ttgtttttct cctcctgaca gataaactcc tttactacca attaaggtat gctctctttc
     7441 gggaaatttt tctaaaatcg attcgcaacc ggctaatctt agcgcgtcaa atactttact
     7501 tgctggcgct tgaggatttg ctatagaaac gttatcgtaa atactctgat tgaatagttt
     7561 cgtatcttga aagacaaaag caattgtgtt aataattgtg tcttttgaat agtgatttaa
     7621 aggcttacca ccaattttaa tctgtccctt atccacattg tagaaacctg aaattagttt
     7681 agctatcgta ctctttccgc ttcctgattt tcctactaat gcataaattc ttttttgttc
     7741 taaaactaag ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc tgacattttc
     7801 aaatgcaatg tcacaatttt taatctgtgt ctcgttccca tattttagtt ggtcttgctt
     7861 catttctaaa taaagggagt ctagtgtttc gactgcatat cttgcttcaa acaagtgcat
     7921 acttacatac attattctca tgaaagaaac aaaaataatt ccacttaaaa aaaacaacat
     7981 caacaagtca acagctaata tcttgggagt ggtaagagaa gttataaaaa aggttatagg
     8041 aactatcaaa atgcaaacta tgccaaaaaa tagccattga aaaagaacaa aaggtgtttt
     8101 cccactcaaa gaatacttat atgcaaactt agcataatcg ttaattgctt tattcaaggc
     8161 tttaaatgat gttactttag caccaaatat ctttataact tgaattcctc ggatatattc
     8221 taccgtttca gagcttaaca catctaacga ttcttgatat ttttgaataa aatttgtatt
     8281 ctctgtcatt ttctttaaca agaatacacc aattactaac atggcgatta gaaccaatcc
     8341 aattttaaaa ttaatgaaaa agcctaaggc aactgataaa acaggtgtta ctatggcttg
     8401 agcactatct ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt tgtcatcaat
     8461 tgtctttcga atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg cttctgttaa
     8521 accgctaatt cccttttttc tcaaattagt ctctaagcga aacccaagga tatgagaaaa
     8581 taagccagaa aaaatataaa ataaagctcc taaagttaac caagcaacag tttgaaccgc
     8641 ataactctta gctaaatatt catctcctac aataattaga gacttaagaa atttgtaaat
     8701 actgtaatat ccatagactg ttaatacgac agaaaaatca gaaaataaag tggctaacca
     8761 tccgtagtat ttcattttag ggacataact aaataatttt ttgtatactt tcattttttc
     8821 ctcccccgca ttcaatttct tctcttattc gtttacaact gttaatgctt tccgtaatag
     8881 cgtaaattag agatgtacaa acagatagaa ggtaaactac gttctatttt ttgtattttt
     8941 ttaagacttt tcttccaatc aatacaccaa tcatagataa acttgctaca actaacactg
     9001 aaatgattcc ccatatacct accgcatagt ggatcattgc atccattgtt gcagcagatt
     9061 gtcctttatc tatccaatct tgacgaaaac gatctgtaaa gaaccatata ggaatccact
     9121 gtccggcagc actgcctaat tgcattagag cccaagacgc aacttgagga attgctcgat
     9181 catatccaaa tacagataga ataatatcag ctaagatcgc catactaatc gaactgataa
     9241 accaaggtaa gtacgccccg cccattaacg taaaaaataa caataaaaca attgtgtaaa
     9301 tagtaaaaat tccttttcta gggtagttat agctaatata aacaaatatg attccagata
     9361 ataatccaaa aatccctggg gatatgctgt gaccaaacgc cccaccaata ctactaacca
     9421 ttattgaaaa aaagtaaatt actagggaaa taactgtagc taaaccaata aaaatataat
     9481 cttttgtttt catatatatt ctcctcaatt ctatttcgtt atgtcattaa aaatctgttc
     9541 tatcaaagtt gaatcttcta ctctaagttt tatatctttt tttattccac cattttttaa
     9601 ataaataatt ctattgcatg ttttataaag aaattccgga tcatgagtgg ctataactac
     9661 tgcacccttt tcttttaagt ctttaataag gtcagcaatc ctttccatat taatataatc
     9721 caacccactt gtaggttcat caaatagaaa taaattagtt tttgacaatg aagctataga
     9781 aaccaataat ctttgttttt ctccaccgga taaatcaaac ggtaattgat ctttcatttt
     9841 ataaagttcc atttttttta aaattttttc ttgctgatct ttactaacta tttcattccc
     9901 cagggctaac tcgctcctca ctgattctgt gaaaaattgg taatccattt cttgcattac
     9961 taaaaacggc gatgacaaat tttttttatt ttcgattatc tttcctttgg tagggcgaat
    10021 aatcttcatg agtattctta gtaaggttgt tttccccaca ccgtttttcc ctaccaaacc
    10081 aataacatct ccaactttga catcaaaatt tatgtctttt agaatatttt cataagtaat
    10141 ttcttcaact tttaatacat ctgcacttac cttttcgata tagggaactt caatcgaact
    10201 attaaaaatg tcaaatttcc ttaatgcgtt attgttttca gtttttaaca actgaatagt
    10261 tgtagttttt acagttctat ttttaacaaa tagaacttga tcaattaatt ctttcaaata
    10321 gtaaaaacga tgttcagcta taacaatagt aaatccttta ttttttaaat cacaaagtaa
    10381 gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa gacggttcat ccaatataat
    10441 taatttagag tttagcgata aactacatcc aatagaaatt ttctgccgtt caccacttga
    10501 tagcgaaaaa atgtttttat ctaatatatt tgaaagatct aataattcag ctaattcatt
    10561 gattctttta tccatatgct cttttgtaaa tccaaagttt tccatcgaaa atactaactc
    10621 actcgttgta tttgttgtaa agaattgtcc tctaggatct tgacttacgt agccaatatt
    10681 ttttgatata ctttcttgtg ttaaggattg tagcgagtta ttttcaaata aaatttcccc
    10741 aaccaatttt ccgtcataaa gtcttggaat aagtccattc attaacttta atagtgtgga
    10801 tttcccactc ccagaagaac cagtaatcaa tgtaagacta ccttttttta ttgaaaaatt
    10861 aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa aaagaaacat tgttaaattc
    10921 aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga
    10981 tgatatagtc taataagctc atgtttgttt tatagtaaga acttcttttc acaggagatt
    11041 ctatcccctt tgttattgca ttagcggtca tttcttcagc aatcatagtt gcacgtataa
    11101 gaacgggaac taacagaaat tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt
    11161 ttctcatttt taaagattct ttaatcattc ttatttcaga acgataagtc ggaaagaatc
    11221 tgatcactac tgtaatactt aacattattt ttttcggtaa atgtattttt tctaaagagg
    11281 ataataattc attgctagta tagacactaa ccaacaacca tgagatcatt acgaccggca
    11341 acattcgaaa tacaatatat aaggatgtcc caaaaaatat agtaaaaaac tctatcttga
    11401 tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg aaataaatac
    11461 atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa caccccactt
    11521 gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca
    11581 aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc gcactgttat
    11641 cttaagctga taaaaatgca ccgttattca gataacagtg cgatcttatc aaaaagcata
    11701 actgagaaca attctcaatg caagcttttt tttaattaac tcagtaattc aatccaccca
    11761 ggaacagaaa attgatacaa tcttctggtt aatagcatag tcttttcttt tgaatagcta
    11821 tgcttcaata tatcatagaa tgtagcgtag aatgagtaaa tgtagaagtg gatttcttct
    11881 tcattaaaga ctaaattttg attgatcatt ccgatagtct ttaacttatc tagacaacag
    11941 actgtctttt ttgtcacaaa tgatgtaatt ttttcaacaa tatcttgata tttggttcca
    12001 gctgattgga ataaaaccaa atcgaatata cgtttattct caaaaatata attaataaat
    12061 ggatctaatt cattgaagtt ctctctccaa cttttctcta tatctatatt ttctaagttt
    12121 aagaggtatt tggtaaactc ttcattatac atattaataa gcgcattaat atgcggctga
    12181 ataatttctt caaataacgc ttccttagaa gtaaaatgtt tataaaaagc acccgtagtt
    12241 acgcctgcgg tcaagcacag ttgccttatc ttagtttgct gaaatcctac tttttcaaaa
    12301 tggcttagtc cactttctat taatttttgg tgagttgtaa tataatctct ggtttctatg
    12361 ggtacttcct cctaataaga tgaattaact atattatctt acacaggact tgaagtttca
    12421 atcgttaacg tctattactg aactcattcc ttagagctaa actaatgaga aagagcaaaa
    12481 taactgatac aaaaagcaat cctacaagaa ttaatagact catctaaatc attcaccttt
    12541 tctaaaataa tccgagaaag atttaaaaaa attatatcca gctattagga taagtcctat
    12601 cataatcact ccaatagcca ttttcttatc ctccctaaaa ataaagcctt ttacttaaaa
    12661 cctttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc
    12721 taaatagtcc tcatatctaa gtttagtcag catctcactt acagatgctg gactaacact
    12781 tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat
    12841 tgctagtaaa taaattagta tagtagttgt tttaatttgt accataccta ccctccaaat
    12901 tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta
    12961 tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata
    13021 ttggtatctt ttgatactgt tttcattggt ctatcgtcca cactgctttc tacgaataag
    13081 gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct
    13141 tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa
    13201 tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat
    13261 ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct
    13321 ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg
    13381 taaataatac cgttttctaa atttaaccaa gcatggggat cttccttccc tttctcactc
    13441 tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc
    13501 tctttgttcg cattttttac taattttgta aaccaagcat ttcctccagt ttccaagtta
    13561 acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt
    13621 tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt
    13681 gctatatttt cagtaatatc tgctaaaatc gaattagtaa ctactacctt taatttttcg
    13741 ttcgaagcca ctgtatcttt gtctttacta tttgttgaac atgccccgaa tataaaaaga
    13801 gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat aactttctct
    13861 ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg
    13921 ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca atgaacaaac
    13981 ctagcaatga tgagcacgct ccgactgtcg ccgaaataaa tatcatagtt tttaaactgt
    14041 ttgcatataa atatgctgta gcagctggtg taattaacat tgcaacaatt aatatagttc
    14101 ccacactttg catagctgtt actgctacta aagttaatag aatcattaat agataatgat
    14161 aaaaactgac attcatcccc attgcttttg ccattaatgg atcaaaagaa gtgattaata
    14221 attctttaaa aaatagaaca actgtaacca acactaaaac agaaacgcca attgtaatcc
    14281 acttatcaat atcttgtaca gctaaaatat ttccaaataa gatatgaaat aaatccgttg
    14341 agctatttgc aactcctata aggataactc ctaatgctaa aaaagaactg aaagtaatac
    14401 caattgctgt atctcctttt attacactat tgctttttat aaaagtaata atgatagaag
    14461 ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata taagaaattg
    14521 ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt cctcttaaaa
    14581 taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta attagagcat
    14641 tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata ttattcccct
    14701 tgtatattag agttagggtt ttaaccttcc ggttttgatt ggataaccct atgaaccatc
    14761 gttattttaa tttggtatta actcatttct tttgagataa tatatttatt ggattgagag
    14821 gctaagccgt tcccccctct ccaataaata tagtatctcc ataggctttt tttaaattct
    14881 cttcattaaa tacagattct acacttccgt gagcaattag atttcgattc aaaagaataa
    14941 tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc actgtttttc
    15001 cctgatgttt caattctttt aacgtatcca taattattcg ttcactaact aagtctatcc
    15061 ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat cttgctaaaa
    15121 gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa tattttccca
    15181 tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta attctttgaa
    15241 aaactttcat tccggcatat gttcccatag atacacattc ttttactttt attggaaaag
    15301 taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt ttattgatat
    15361 cttcattatt aatggagacc tctccctgat gagggataat atttaacatt gctttaagca
    15421 atgtagattt tccagcacca tttggaccaa taatacctgt taaagcacct tcttgtattt
    15481 ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc tttttcactt
    15541 ctaacataac tttctccttc taaatgataa tgattatcac gatcatatgg tatacaaaaa
    15601 ttatatttta gtcaacccta aattttataa tttagatttt tttctgttgt tgaagattag
    15661 gatgtcaaaa ttatttgaaa ctactcgtat agtaaatagt aagtaatttt caaaacacga
    15721 agaaaaacaa aaagaaaaaa ggaaacgccg aagcatttcc tttcagtgat ttaatgagaa
    15781 caacacgatt tgacttagag tctcaaaaag tcgaataggt acactcattt aagtgtctcc
    15841 attcgacttt ttggggatga gctggctatt gaggtccgct taccctattg aataaataca
    15901 agattgggta tttctaataa gtccgccctt catattgggg tgatcggggc gaacatgcca
    15961 tcttttcata taccacctcc tcgacactca ataatcaaag attctcgtta atcttcttcg
    16021 atgcttgcaa tgatgttttc aatattcata taacgaaaat cttcttgttt gatataaacg
    16081 atctgagcgg tacatttttc ctttttgatg atttctttta ctttttcctc agaacgtgcg
    16141 acctgcggcg ttaaaagtaa aacgtcatag tctttataat tcagtttttc ttcaacgttt
    16201 cggatcgttg cataatctat ctcatacgat agatttttgg ctctgatcgt ttcaagcatt
    16261 tttgatacga gcagcgacgc actgattccg gtctcgcaaa taatcaatag atccttatgc
    16321 ataataccct cctgttttta gcctgcaaag ccaattggtg aaaatgccac ataagttaaa
    16381 ataacaatga ctaccaatgc tatagcagtc accttggtgt atttccaagg ctcgatatcg
    16441 acctttgtat cgaatgagtt caacacaaat tctccatcag gtttcaattt ggaaaccacc
    16501 atcaaaatga taatgtccag aacgaataat gccgtcaaaa cataaaggaa atggatattt
    16561 tggaaaagca tttttgatag tgcatacaag acgatatgca cggcaatcgt cgtttttgcc
    16621 ccaaaagcag ttgctttttt tgaataaaag gcaaagagaa tgatcacgag cagcggcatg
    16681 ttgtatagtc cattgaattc ttgaacgaac tgatacaatc cagccggtgc aaaagaaatg
    16741 aatggcgcta cgataatgga gataatcccc acaacgaccg ttactgtttt accagcacga
    16801 gcgatttgct tatcatttgc tttcttattg ataagcggtt tataaaaatc cagtgaaaac
    16861 aaggtcgatg tcgcgttcaa agctcccgca aatgatgata agatcgcgcc aaagataaca
    16921 gcggcgaaaa taccaaagat cattttaggc aatacttcac tgactaaaaa aggatacgcg
    16981 ttatctggat ttgacattaa actatcgcca aaaatattgc gagcgacgat ccctggaaag
    17041 acaaggaata acgcacctaa gattttgaag acgccgacta ggaaagcgcc cttttgtgcc
    17101 tcagctaaat tcttgccgga caatgctttt tggacgatca tttgattggt acaccagaaa
    17161 tacagattgt taaacagcat ccctgtaaat agtgtcggcc atggaacaac agccgagtcg
    17221 atcgatccca cagagttaag cagccatggc gtgtttacca caacgtgttc gatcccgcca
    17281 acaaagctgc cgtcaccaag cttcattaag cctaaaattg ggaccagcag accacaagcc
    17341 agcagaccga ctccataaat ggagtcacta ttggcactta acgacaagcc gccgatcagc
    17401 agatagagga tgccgacaat accaattgcc atcgctacaa ttatgatcgc gacgatcggc
    17461 tggacgccca gcagctcatc aatatggaag atcttattga acaccagcga tccagaataa
    17521 agaaccacgg gtaagaaaga agtcatatag gtcacaataa ataagatcga aataattcgt
    17581 ttggtaaccg tatcataacg aatctcgatg aaatctgaga ttgtattgat gccgtattta
    17641 aaatattttg gcaagaatat cacagccaaa gctacgatcg caatcgtagc agttacttcc
    17701 caggccatta cttccatccc tgcatgataa ctttgaccat tttgtccgac tatttgttcg
    17761 gtggacaggt tcgtcatgat gatcgtgccg gcgattggca aagcagttaa gctccgccct
    17821 cccatgaaga acccttctga tccagaagta tcgacacttc tggatctaga ataggcaaat
    17881 aaccatacac agatgacaat cactaggaaa ctgattatag aaaaaagatt catcgtctag
    17941 ctccttttca atagattaaa gaccggtgac ctctcgaatg tagcttctgg ctttgactgc
    18001 atattcaaat ggattggctt tagccgggtc ctgttctgct tcaactacga tccagccttc
    18061 atagtcgctg gcatggattg ctgtccagat tggagtgaaa tcaatcatcc catcaccagg
    18121 aaccgtgaag acgcctttct tcacgccgtt taagaagctg tctttttcct tttctacttc
    18181 ttcagccgtt tcttgacgaa tatctttgaa atggatgtgt ttgatacgat cttgatgacg
    18241 gttgtaaacc gcgattggat tttcaccaga aaagaccaaa tgtcccgtat cgaacaataa
    18301 tttaactttg tctggatcag tcatttccat taaacgatcg atttcttccg tcgtttgaac
    18361 gccggtaccc atgtgatgat ggtaaacgat ctccatatcc ttttcgtggg ccaaatcccc
    18421 taatttttct aatccttccg ccagtttttt ccactcttca tcagtgaaaa ctggtttatc
    18481 atcaaagact ggcgctgtca taatcccttg gatactatga ccctgctcag ctactacgat
    18541 gactttagcg cccatcgcat gtaagaaatc ccgatgtgct ttgaaggctt cggcagtttc
    18601 ttcaaaaggt ttagttgtca gaaacgcact gaaccatgca ctggcaacct ttaagccgcg
    18661 aatattcaaa tattgttcca acacctctgg atcttttgga tatttgttgc caatctctgt
    18721 tccggtgaac ccagcaaggg ccatttcact gatacattgt tcaaaggtat tttcactacc
    18781 aagctcgggc atatcatcat tggtccacgc gattggtgcg atggctaatt gaatttgttt
    18841 ggtcatattc tcacactcct gcttattttt ttagttgtac taaactcttc gtatcgaatg
    18901 attcttgaca agccaacgcg atcttagttg attgcaatcc atcttctgca ttaaccgctg
    18961 gttgacgttt ttcttgaata catgagacaa attcttgggc ttcacgaatg aaggcttctc
    19021 tgaatcgttc tgggaagttt tgtgaacatg gacggatgat cccttgatcg ttcatgatcg
    19081 tcacaagatt tttctcaggc gcttgcgcta cccgcagcat tcctttggtt cccatgatct
    19141 ctgtttcgac atgatagcca tgggctgcgt ttctgccggc aactagtaaa gctattgttt
    19201 tatcgctcag gctcatcatt gcggctccag tttcaagctc accagcctga tccaattctg
    19261 gatacgccgc atttttccca atcgcccaca cttgatcgac ttcttggccg gtaaaccagc
    19321 gaaccaaatc aatatcatgg atcgccatat ctaagaacaa gccgccgctg tcgttattca
    19381 tggcaaattt gacaaagctt tccaagcctt cgctcggatc gatcccataa caacgaatca
    19441 gtgtgatttc gccgatttcg ccatcttcta ccattttctt cgcatactga tacgactcat
    19501 catagcggcg catgaagcct aacatgaaga cctgttcagg atgctcttcg atgacttttt
    19561 gggtcttttt aatatcttca acttcaaggc ctaatggttt ttcagtaaag acatgcttgc
    19621 cattttccat cgccaagcgg acttggtcac agtgaaagcc tgatggtgaa acaataaaga
    19681 ctgcatcgat cacggaactt ttgaccattt cttcatagct ggaaaaagta tgcttgacgc
    19741 ctaatttctt ctgtgcgaat gctaattctg tttcaactaa actgcacgca gccgtaagct
    19801 cacaacaact gacacctgtt gctaaatttt gtgcatgtac ttttcctaat cttcctaaac
    19861 cgacgatgcc tactctaatg ttgttcatga aatacctccg ttttctgaga attactcatt
    19921 gtatagtgct ggtcgttcat ccatttgaat cattactttt gtttttgttt cacgagactc
    19981 atgacaagcc atcatggtga tcgctgctac atacccatcc caagcggtag gaccgacaac
    20041 cgcatcattt ttgatcgaat cgatccacag cttgaattca taatcatagg catccaaaaa
    20101 gcgggtctgc caatctggtg aaacaggtgt gtaatttttt tcttccgatt taattcgtgt
    20161 ataggctggg tccgtcattc ctacttctgc cgtttcgaaa accacatcac atttaatatc
    20221 gtagccaaag cggcaattca caaagatttc caaatcaatc gttacaccgg attttgtttg
    20281 aaagatcagc agctgcggat catgcaaatc ggcatgggag ttgctggttt ttttcggcaa
    20341 gatcatttga acagattcat aatcttcatt tagcaaccag cgaagcacat caatctcatg
    20401 aatcgcggtg ttgctgatcg ccatcggtgt tgtgtagttt tcatctgcgc taggatttct
    20461 atgggcacaa tgcatcatta atgcttctcc gtattgcttc gattcgattg cttctttcag
    20521 ttcaatgtag ccgcggtcat agcggcgcat gaagccaacc tgaaccaatt tcttcccata
    20581 agcaacttcc gcatcaacga tccggcggca gcctgctgca tccatcgcta atggcttttc
    20641 gcagaaaaca tatttcccat gcttgattgc ttctaacaca tactgctcat gggtcgggtc
    20701 ccaagaagtc acaacaacga catccacatc ttcagaagcg atcaatgctt ctccggtttc
    20761 aaagaattta gcatcgacac gtttggcaac ctctttagca gcttttacat tgatatctga
    20821 gactgctgtc acataagccc cttgtgtttt ctgattgatc ctgtcgatat gctcgatgcc
    20881 gattgcacct gttccgataa cgcctacttt tactctcaat tacttatccc cctgttctgt
    20941 tgtttaagca cacatagtat gcgcttacaa aattattcct gtgtagcttt tattcttaaa
    21001 aggtagagcc ctctctttgc tccgcctttt actacccata agcttgtacg tataattcct
    21061 taatttgact atgactaggc acctttttat tattttgcgg acttccgctg gtcaacgcat
    21121 ccgcggccat tttgtctaag gctgccataa acgcttgctg atcaatacca taatctcgaa
    21181 tcgttgggat cgacagctgc tcacagagct cttggatttt tttaatcaga taatccgcag
    21241 cctcttccag cgataaatct tcggctgccg gataaataaa tctggcgatc gtcgctaact
    21301 cagtctgaat agcatccttt gagaattcaa aaaagaccgg caataacatc gcatttgata
    21361 tcccatgagg aatgtgaaaa agtgccccca ccggtcgact cattccatga atcaatgtga
    21421 ctgacgcatt cgaaaaggca atccccgcct ccgttgaagc taagctcatt tgttctctgg
    21481 cttcagtgtc gtcgctattt tcatatgcct gagaaagcgt gttcaaaatt ttcttcatcg
    21541 cttccagtga aaatagttgc gttacttcat tggctttgac tgaaacataa gattccaaag
    21601 cgtgacatag cgcatcgact cccgttgccg cggttacttt tggtggtaca gttttcgtaa
    21661 aatcagagtc aacgattgag atctgtggca tgaaaacgag atccttcatc atcattttga
    21721 ctgctgtttt tgtattggta ataactgcca catcggttac ttccgaacca gttccagcag
    21781 ttgttgacac tgcaatcagc ggaatgcccg cttgcagctg atcaaaattt cttgcatgaa
    21841 tcgcttcgag acttaattga ttagcagcca aaacggctac caccttggct gtatccagac
    21901 agctgccgcc accaaaagca atgatatgat cacattgatg cttctgaaaa agcttcagcg
    21961 catcataaac attttcttgc gtcggttcgg cttgaatgtc tgtatactcc gctaattgat
    22021 catctgtttt tcccaacagc tccttgatct tttgaagatg cccggtctgt tccataaccg
    22081 tatcactaat gattaacggc ttgttgccta gttgttctga ttcttccata agaaattgga
    22141 ttgttccgcc gccaattctt aaattggcag gcattttcag ttgaatattc acagaaaaaa
    22201 cctctctctg gcttatagcc tataacagaa tcctcgaagc tctttaacag agcttcgaag
    22261 caaatcttta aatctcactt tgcgcataac gtgctgtaac aactttccga tgggtataaa
    22321 actcaacact gtctttgcca ttggcatgca aatcaccaaa gaaagagtgc ttccagcctg
    22381 aaaaggcaaa ccaagccatc ggtgcaggca ccccaagatt gacccccagc atcccagcat
    22441 caacattttc tctgaagtag cgaatcgctg atgcgttgtt ggtatacaga cacgaaccat
    22501 tcgcaaattc agattcattc gtcagctcaa cggctgccat caaatctttt acccgcacga
    22561 ttgatgcgac cggggcaaaa atctcatcct gccagatctt catatccatc gtgacattat
    22621 caaaaatcgt tggtccaaca aaatagcctt tttctggaat gttttcgcga ccatctagta
    22681 ccagtttcgc tccttcatcg accccttttt ggatatagtc aaaggtccgc tgctgtgcat
    22741 cttttcgaat cactggtcct aaaaagaccc cttcgtccaa gccattgccg atcttgatcg
    22801 cttttgccgc agccttaaat ttttcaacaa actgatccgc gatttcctct tccaaaacaa
    22861 tgacggaggc tgccatgcag cgttcaccag cagaaccaaa agctccgcca ataaggtcct
    22921 tgactgcccg atcaatattg gcatctttca gcacaacaac atgatttttc gctccagtca
    22981 acgcttgtac ccgttttaga ttttttgttc cttcacggta aacgtattca ccgacatttt
    23041 ttgatcctac aaagctgatg ccttttacca gtggatcttg cagcaatgtg ttgacaacat
    23101 cgtgggcacc atagacaaca ttaaaaacac cgctaggaaa gcctgctttt tcaaccagct
    23161 caacaatttt ctccatcaac aatggtgttt tttctgaagg cttcagcacc accgtatttc
    23221 ctgccgccag agccatcggg aagatccaga agggcaccat cattggaaaa ttaaacggtg
    23281 tgatccctcc gattactccg attgggtaac gataattcgt gatctcaata tcagtcgccg
    23341 ctgttgataa agaatctccc atcattaaat tggtgattcc cgctgcgtgc tccacatttt
    23401 cgattcctcg ttgaacttct cccagtgact cggacaatga tttcccattt tccagcgtga
    23461 tcaaacgcgc caattcactt ttatgatctg ttaaaagctg atgcagcttg aacagataac
    23521 gtccgcgaac aggaacagga acttttttcc aagtttgaaa cgcagcatcg gcagctttaa
    23581 ctgcttcggc tgtctctgct ttgctagaca acggtacatt cgccagcgct tcttctgtag
    23641 ccggattcat tacttcttca aactctgttg agcgactagc gacccaacga ccatcaatat
    23701 aatttcttaa aaatcttgtc attt
//
LOCUS       DASJON010000022        23558 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJON010000022 DASJON010000000
VERSION     DASJON010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.022"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..243
                     /locus_tag="U3989_000673"
     CDS             22..243
                     /locus_tag="U3989_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1714141.1"
                     /translation="MKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHEV
                     DMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            522..1022
                     /locus_tag="U3989_000674"
     CDS             522..1022
                     /locus_tag="U3989_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1714142.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1105..1656)
                     /gene="lepB"
                     /locus_tag="U3989_000675"
     CDS             complement(1105..1656)
                     /gene="lepB"
                     /locus_tag="U3989_000675"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1714143.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1808..2035)
                     /locus_tag="U3989_000676"
     CDS             complement(1808..2035)
                     /locus_tag="U3989_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN1714144.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2032..2550)
                     /gene="msrA"
                     /locus_tag="U3989_000677"
     CDS             complement(2032..2550)
                     /gene="msrA"
                     /locus_tag="U3989_000677"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1714145.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2570..3283)
                     /locus_tag="U3989_000678"
     CDS             complement(2570..3283)
                     /locus_tag="U3989_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN1714146.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3286..4182)
                     /locus_tag="U3989_000679"
     CDS             complement(3286..4182)
                     /locus_tag="U3989_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1714147.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4340..5182)
                     /locus_tag="U3989_000680"
     CDS             complement(4340..5182)
                     /locus_tag="U3989_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1714148.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5314..5967
                     /locus_tag="U3989_000681"
     CDS             5314..5967
                     /locus_tag="U3989_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN1714149.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6030..6557)
                     /locus_tag="U3989_000682"
     CDS             complement(6030..6557)
                     /locus_tag="U3989_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1714150.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6577..7524)
                     /locus_tag="U3989_000683"
     CDS             complement(6577..7524)
                     /locus_tag="U3989_000683"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN1714151.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7536..9422)
                     /locus_tag="U3989_000684"
     CDS             complement(7536..9422)
                     /locus_tag="U3989_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1714152.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9582..11660)
                     /locus_tag="U3989_000685"
     CDS             complement(9582..11660)
                     /locus_tag="U3989_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN1714153.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11673..11948)
                     /locus_tag="U3989_000686"
     CDS             complement(11673..11948)
                     /locus_tag="U3989_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714154.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12310..12765)
                     /locus_tag="U3989_000687"
     CDS             complement(12310..12765)
                     /locus_tag="U3989_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714155.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12919..14127)
                     /locus_tag="U3989_000688"
     CDS             complement(12919..14127)
                     /locus_tag="U3989_000688"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN1714156.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14239..14571)
                     /locus_tag="U3989_000689"
     CDS             complement(14239..14571)
                     /locus_tag="U3989_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1714157.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14719..15594
                     /locus_tag="U3989_000690"
     CDS             14719..15594
                     /locus_tag="U3989_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1714158.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15676..16257)
                     /locus_tag="U3989_000691"
     CDS             complement(15676..16257)
                     /locus_tag="U3989_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN1714159.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16263..17522)
                     /locus_tag="U3989_000692"
     CDS             complement(16263..17522)
                     /locus_tag="U3989_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1714160.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17524..18570)
                     /locus_tag="U3989_000693"
     CDS             complement(17524..18570)
                     /locus_tag="U3989_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714161.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18805..19080)
                     /locus_tag="U3989_000694"
     CDS             complement(18805..19080)
                     /locus_tag="U3989_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN1714162.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19323..20633)
                     /gene="der"
                     /locus_tag="U3989_000695"
     CDS             complement(19323..20633)
                     /gene="der"
                     /locus_tag="U3989_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN1714163.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20815..22044)
                     /gene="rpsA"
                     /locus_tag="U3989_000696"
     CDS             complement(20815..22044)
                     /gene="rpsA"
                     /locus_tag="U3989_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN1714164.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22179..22859)
                     /locus_tag="U3989_000697"
     CDS             complement(22179..22859)
                     /locus_tag="U3989_000697"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN1714165.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(22908..23543)
                     /locus_tag="U3989_000698"
     CDS             complement(22908..23543)
                     /locus_tag="U3989_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1714166.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
BASE COUNT         6546 a         4874 c         4004 g         8134 t
ORIGIN      
        1 catgattttt ataaaaaatt tataaaagat aaagaaatag ctgataataa aaaaacaagt
       61 ggtgctttga tttcgtttag tgttgaaaat gcggatgaag taaaaaaatt tgctcaaatt
      121 gcaaaagaaa atggtggtac tttccacgaa gtagatatgg gaatgcctga agaagaaatg
      181 ttttcattag aagtccaaga tctagacgga aatacattag aacccgtatg gatgaagatg
      241 taagcagata actaatctga cgttttttat aacaaaaaaa caaaggcgga tgatacgata
      301 agtattctcc gcctctattt tttttattcg tacaatctct tttttacagt ttataaaagc
      361 agtcttctca cccttttttt aattgttaga tgccagtgta tgtaaatcac caatgtgtca
      421 tgacacattg gtgattttat ggtatccttt tattattaac tgacaaagga gagaatattt
      481 tgaacacttt gattgcaaaa gacgttttta gattgcccga aatgaatgca agattgttgt
      541 cgacctctgc tcttttatta gcccttgatg tcatcttata caagatagcg attggtcctg
      601 cttattttca aatcacattc ggatttttaa gtatggcgct tatgggttat ctatatggac
      661 cgatctgggc tggtttgctt gaaatgttgt cgaacacttt aaatttcacg atatttggtt
      721 caggaacgtt tcaagctgct tttcttatca cggctttcct tggtggattt attaatggct
      781 tatttttata tcataaaaag ataaacctgc cttccgtcat cgctgcccaa ttattgatta
      841 tgatcccagt cagccttttt atcaattctt ggctgatgtc tgttttgttt ggtgcagatt
      901 tcaaagtaat ttttctaggt agattcatca ggaatatgat ccaaatccct attcaaataa
      961 tggttgttta ttcatttatc aaaactttag aggctagaaa agtgtttaaa cgagctgttt
     1021 gattttacgt actaaggtag taaactgatt ttggattaaa caataaagcg tgcagtctca
     1081 acagactgca cgctttattg tttctcactg gatccatttt atttcatcaa gtggataata
     1141 gacaaatctt gctttaccta gaatatcagt tttattgatc gtgccaaacg aacgactatc
     1201 ttttgaaact cttcgattat ctccaagtac aaaataatta tcttccggaa gcttggattg
     1261 tcccgttagc tcttgtagtg taaagtccgt cgtataggat gcatttgcat gatctgattt
     1321 caggttttta gttagataag gttccttaac tactttccca ttgatcttca attgatcatt
     1381 ttggtagctg acattttctc ctggaagacc gattactcgt ttgatataaa tcgtgccatc
     1441 tgctaattga aaaacaacaa tatcaaatcg tctgatctcc gaaaattttt ccatcacaac
     1501 catatcacct tgcttcaaca cgttttccat tgaattgcct tccactggta ccgggatgaa
     1561 taaaaatccc cgcaacatga acgcaaggaa tacagaaaca agtaagtatt tgaatacaag
     1621 ccagaagcga ttaatatagc gttgtttttt tgtcaatctc tatcaatcca tccctctttt
     1681 tttcattctt tctttattat aagaaaaata tcaaaaaaat gcgactagat agctgaaata
     1741 aaaataattc tttcaattag gctattggac aaaaaacttt tttgatcata aagtacaaaa
     1801 ctatctctta tatgtcatga ttattttcta aatatttttc ccatgcttcg tcaaagatat
     1861 ccatggacgg caaatagtcg acattcagtt ccagataatt agaaatttct tcgtagctag
     1921 agctgtgctt aggaaattgg atgtctttac cggcttctgt agcgaaaaca gataaagcat
     1981 catgtgtgcc accgcctcgc agtgtcatta aataatgaaa aaagcttcga ttcacccttc
     2041 gttcctccaa ttttcttcga taaaggcagc ccggcgtgca tggtttcttt taaaattttc
     2101 aggatgcttt ttataatact cttgatggtc ttcttctgct agataaaatg gttgggctgg
     2161 ttcgattttt gtaacgattg gctgttcgaa acgtccactt tcagctaact gtttcttact
     2221 tttttcagca atgatccgct gttcttctgt caaataaaag atcaccgggc gataattgtc
     2281 accgcgatcc tcgaattgtc cgaaagcgtc agttggatca gtttgctgcc agtaaagttc
     2341 tactaattgt tcatatgaaa tcaatgttgg atcatagata atctccactg cttctgtatg
     2401 tccagtcgtt tggcttttta cttgttcata agtcggatct ggtacatgcc ctccagtata
     2461 tcctgacatc actgtatgga taccaggaag cgtgtcgaaa ggctggatca tacaccagaa
     2521 gcagcctcct gcaaatattg ctcgttccat tatgattccc cctctaatac tactcatccg
     2581 atgatttttt attttctttt gtcttgcttt cagtactgct ggtgctagtt ttgttggtat
     2641 ttgagttgtt tgaagcatca gaggtatctt ttggcaaata tagactaaac cgaatgtcat
     2701 catttactaa gtcgatttta tctgctttaa tatacatccc gttttgcatt cggaattgat
     2761 ccaacctaag catgatagtc agatcatctg tattgacttc gatccaatct ggtaatttat
     2821 agtttctctt gatcatattc atcacatctt tgattggcaa actcaatgtt ccgatagaaa
     2881 gacttctagc ttttagttgg acatttccgt tttccatcac atacggatca aaatataaat
     2941 agaaatcgat aggaaaacct agtacttcga aagtaccatt gagtagtgct tcattttcca
     3001 aataaaattt atattcaata tcactatctt tttgatactc actaaggaaa aagctgatga
     3061 tttgattgac tttttcttta tttgaattga tcgttaagac aggatctcct tgtctttccg
     3121 taagagctgg tatttctggg agttctggtt cccgattagc aaaaatacgg ctgcctaaaa
     3181 atacggtcga accaagtact accccaagaa ggataaggaa ggcgaatttc cagtaatttg
     3241 ttttctttgt tttgtttgaa gatacgtcag atcttgtttt catgtttatt gatcatcctc
     3301 cttctctaac cattggtttt gtgtctcgat cagctgatct ctcactgcat ttgccatcaa
     3361 ttgataacct aaattatttg gatggaaatg atcttcttca tagagcacat tatttttgat
     3421 gccatttaga ttttctgtcg tgctagttgt ttgatctgtt ccttcttctg tgacacctac
     3481 ttcctgattc aatccttgat aaagcaaatc attgattggg ataaagtagc agttcggcat
     3541 tttttttgtc accgtttctg ttcctttgtt ccaattgtta acaattgtct gcatgtcagt
     3601 gatctcagga aagttcaaat aaaatggatt gtagatgcct aaaatgtaaa taggtgcggt
     3661 tttattgagg gatcgaaatt cttccaatag ttctgtgaca ttttcctgat agtttttcag
     3721 tggcttttta aaggaattga ccgttaaccc gaataaatta ttttggataa ctttcatcaa
     3781 atcattaccg ccaacagtca gtgtgataaa atcagctgtt ttgatattgt ttcgaatttc
     3841 ttcttttgtt tttactcgct ttaagatctg gtcacttcgt tcaccattta caccatagtt
     3901 ttctgtttca accgcggtca aattataacg gtcttttaga tcatttgcga caaggggaac
     3961 gaatcctcct tgcttggttt gatctcctac tccttcagtc agcgaatctc cgatagctat
     4021 gtagtgaagt ttttcttttt tatttggatc ttctctcaca gtagttactg gtttcattag
     4081 tgggccagct ttcggtatag aagcagctaa acctgcatag atgatgatcg caattaataa
     4141 tgggaacaaa acgaggatca aatgtcgttt cgttaatttc attttttcac ctcttgtatt
     4201 cgattggttc taatgttatc aaaaatttta tcaaaaaggt agtctttatg aaaaaagaaa
     4261 acaattattt tcctaatttt ataaagaaaa agagaataag ctgcaagcct ttcaggctga
     4321 gggcttatcc tcttttcgac taatctacat aatacatgat tgcaaacgcg tctttaccag
     4381 tatgcgtagc gataaccgga ttcgtatgca gtacagggat atgcatgtcc gggaataatt
     4441 cttgcaaacc ctctttgaat tcttgcgcca attcttttgc atccgcatgt gaaataccga
     4501 tttgtcgcac atttggcaat tctttcagtt catttttcaa attattaaac catttatcga
     4561 acgttttctt tcctcgcccc ttagttacag ggattaattc agaatgggcg aaattcatca
     4621 ccacgcgtat gttcaaaaca ctagacagca aaccagttgt tcggctgatt cttccacctt
     4681 ttaccaagtt atctagtgtg gatattccga tgtataattt tgtattgtct tgtacttttt
     4741 tgacttcttc aaggattttc tctactgttt taccttcttt tgctaattca gctgctcgaa
     4801 ttacctgaaa agataatcct tggtcagtgg tatcactgtc gataaccgtt actttcgttt
     4861 ttgttagatt actggcttgt cttgctgctt ctactgttcc actcaagcct tttgtcatat
     4921 ggatcgaaat cacttcactg ccgtcttctc ctagctcatc atacatcgag atgaattctc
     4981 caattggtgg ttggcttgtt ttgggcagat tttttgcttt cgccatcatt tccataaatc
     5041 tttcgccgtc tagttggtca tcatcgggat atactgttcc gtcgatcatg atggatagcg
     5101 gcatgacacg aatgcctaaa gcatcgcgta catttttttc catcgtacat gaagaatctg
     5161 ttacgatttt tacatttgtc attattttct ctctctcttt ctttaacaag atgccttatt
     5221 catgttaaac tacatgtaag acaatttcta tttttagtat aacagacaaa taatgatttt
     5281 tcattaatta cacctagtaa aggagccgtt ttcatggaaa aaacgaaatt taatcgaacg
     5341 tatgaaatat taaatgaagt gtttaatgca gtcacccatg gaattgggac tggattgagt
     5401 atcgcgggac tagtggttct tttagtaaaa ggtgcccact tgcattcctc cctccatatt
     5461 gtctcttatg ctatctatgg atcgatgatg attcttttat ttttaacgtc tactttgttt
     5521 cattcattga tttttacaaa agcaaaaaag gtgttccaag tattcgatca tagttcgatt
     5581 tttcttttga ttgcaggaag ttatactcct ttttgtttgc tgagtatccg aggatggcta
     5641 ggttggactt tatttgtatt aatctggttg tttgcgattc ttggcattat atataaagcg
     5701 ttgacgttgc ataaacggga gagagtatca aaagtatcta cgatcatcta tatttttatg
     5761 gggtggctct gtgtcgtggc tgctgttcct ctttatcact ctcttggaat gactggtgtt
     5821 attttgatgg tagcaggcgg gctttcttac acgataggcg cattctttta tagcttgaat
     5881 tctatccgct atatgcatgt tgtttggcat ttgtttgtta tgttaggtgc aggttttatg
     5941 tacttctctg ttttattctt tacttaataa aaaaaataaa gctatggatg ggacagctct
     6001 aaataacagc tagtctatcc atagcttttt tatatcgtca attcgcctgt cccgcttttt
     6061 acaggattct cattacgctg atacgtttca aattgatgcg gataacgatt tttttcatcc
     6121 atttctcctt gactagtatt gatcagtgac caatcagacc agtcaacagg tggaaaataa
     6181 gcatctccat cgaatacatg atggatgact gtacggtata atatcgtaca aaatggcaga
     6241 aattcttgat aaacagctga ccctccagcg atgaaaaaga taccgtctgt ttctttttcc
     6301 agagcaagca cgtcttccac actatgcatg acatgcacgc cttctgcttt ataggaagaa
     6361 tctctcgtca aaacaatcgt ctgacgattc ggcaacggac ggctacccat cccttcgaat
     6421 gtttttcgtc ccatcactag tgtatttgct tctgtcattt gtttgaagaa tttcagatca
     6481 tttggcaaac gccaaggaag acggtcttct tttccaatca atccgttttc atcctgtgcc
     6541 caaattgcag ctaacatttt ctcatccttt ctgattctaa acagcgatag gagctttgat
     6601 cgccggatgt gggtcatatc cttctaattg gatatcttcc atctcaaaat caaatactga
     6661 ctgcttttcc tgattcaaaa ctaaagtcgg aaatgcgcgt atttctcggc tcaattgttc
     6721 tttcatttgt tcgatatgat tgatataaag atgagcatct cctaatgtgt gaacaaattc
     6781 gccaaccgct aaacctgttt catgtgcaat taggtgagtc agcaacgcat aacttgcaat
     6841 gttgaacgga acgcctagaa acacatcccc actgcgttga tacaattggc agcttagccg
     6901 tccctcttga acataaaatt gaaacatcgt atgacaagga ggcaaagcca tgctaggaac
     6961 atcttccgga ttccatgcag aaacgatcaa tcttctagaa tcaggagtct ttttgattgc
     7021 ttcgatcact tgtttgattt gatcgataaa gccaccgtct tttgtttccc agtgacgcca
     7081 ttgatagccg tagatgtgtc caagatcgcc gtatgtttca gcaaaggaat catcggttag
     7141 gatcttctcg caaaaaagct tgctctgctt tttatattct tcattgaatt caggatctgt
     7201 caaacatcta tggccaaaat cagtcatatc tggtccatta tagtctggac ttttgatgta
     7261 gcgttcaaat gcccattcat cccaaatatg gtttttattt tgtaatagat agcggatatt
     7321 cgtatctcct tttaaaaacc aaagcaattc gcttttgatc aatccaaaag gtactcgttt
     7381 cgttgttaac aaaggaaacc ctttttgcag atcaaaccgc atttggtatc cgaataagct
     7441 taatgtgcct gtgcctgttc tgtcttcttt acgatgccct tcttccaaca attttcttcc
     7501 taagtcaaga tacgcttgtt ccatcctttt tcctcctaat cttctaccca ttcactcaag
     7561 tattcccacc gtgccatttt ttcttcaagt tgtgcttctg tttcagatac gtcgttttgc
     7621 aattcttgta atcgagtgaa attatctcct tgatgattca tctcttcttg gagcaatgag
     7681 attttttctt caagttctgc aatctcatct tcaatcgttt cccattcttt ttgttccatg
     7741 taagataatt tcttcttttc agcaggttcc ttgctctgta tttttggttt cgctttttca
     7801 gcaggctgct gggtctcttt ttgcttcagt aaataactgg acatgtttcc gtaataaagt
     7861 tcttgttttc cgtttccttg gaatactaat aacttatctg ctaccttgtc caagaaataa
     7921 cggtcatggg aaacagtgat cacagcacct ggaaaacttc taaaataatc ttccaaaatc
     7981 gtcaatgttg ctatgtccaa atcattggtc ggttcatcca ataataaaac attaggttgc
     8041 tgaatcagca gtttcaacaa aaataaccgg cgtttttctc cgcctgaaag tttgcctatc
     8101 agtgtcccat gcataaatct tggaaataaa aatcgttcaa gtaattctgc tactccgatt
     8161 tgactgccat ctgctgtttt tgcttcttca gcagcttctt gtaagtaagc aatcatccgt
     8221 ttatctggtg ccatttcttc attttcttgt gtataatacg caagatgaac cgtttcaccg
     8281 atagacattg taccgctatc caggggaatt ctgccagcta gaatattaag caatgtcgat
     8341 tttcccgcac catttttacc agtaatacct agtctttccc gagattgaat caataaatct
     8401 aaatgttcta gcagtgtttg gtcatttata cggtaactgc catctttgat ttccagtact
     8461 tttttcccta aacgttgtgt cgctaggttc agctctaaat catcttcttg gtttacttgg
     8521 tacaagttct cctttagatc ttcgaaacgg ttgatccgtg cttgctgctt ggtgccacgt
     8581 gcctgtacgc cagccctcat ccaagctaat tcctgtttat aaagccgctt gcgcttttct
     8641 tcttgttcta cttctacacg atcacgttct gctttttcta agacgtaagc ttcataattt
     8701 cctttatact cgtataattt tccaaatgaa agttcgaaga ttcggtttgc tactctatct
     8761 agaaaataac gatcgtgggt aaccatcaat agtgcgccac ggtattgttt caaatagttt
     8821 tccaaccact cgatcgcttc ataatctaaa tggtttgttg gttcgtctag taatagtaaa
     8881 tcgggttcat caatcaacac ttgcgccaga ctaactcgtt ttttctgacc acctgataaa
     8941 gtactgattt ttttatctaa ttcactgatt cctaattttt gcaaaataat ctttgcgttg
     9001 gtatctgtag tccaagcatc ttctttattc atttgttctt ctgctttagc ataacgtttt
     9061 tgcacatcag ggtcttctcc gttttcccct agttcgatca atgcttcctc atatgctcga
     9121 acagtttgga tcaaggggct gtttccttga aagactgctt ggagaacagt taattcatcg
     9181 gaaaattcag ctgcttgaga aagataacca atacggtaat cagttggata aaacactgtt
     9241 tgccgatcac cgtcgctgct atcgataccg gcaataatat ttaagagact tgttttaccc
     9301 gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt ggaccaaaaa agaaatctgg
     9361 tcaaacaaat ctttttcacc ataagttttt ttgaggtcag ttacttttaa ttctttcaac
     9421 atgttccctc cttatcttgg atattgtatc ataaaaatag tgaacttcag gaaaattttt
     9481 ttggtatttg ttctttttgt gttctcaaaa aaagaacagg accgattcga tcctgttctg
     9541 atatgttttg ttttttataa aacttgaaaa tttttacctt tttattttat tggactcgtt
     9601 ttttttgact ttgctcgaaa cgcaaatcgg aaagaaaagt tatcagatgg tttgttcatc
     9661 gtataagatt ctaacaaatt gatattcctg cgatagctgt tcgcagaaaa cgtcgtgatt
     9721 ttttcagcaa cttcgtattc atcaatgatt tttctttcaa gataataacc tcttagcatt
     9781 tttttcgtaa acagactatc ttgctggatc atccacgaag aaatcaaatt ccccgagttc
     9841 atttttattc cttcaagttt gcgttgctgg ataaaaaatt gaaccatcgt ttcatcataa
     9901 atatcagtca agttatctaa actttcaata atcaactgag tatttttttg atatgtctct
     9961 ctgatttctt ggatatcttc atcactaata aatgtctgac gatcagcata tcttctttgc
    10021 caaaacattt tcggatggag aacgattcgc attaacctac ggacaacaat gaaccaaaag
    10081 ccgataaaag acaaaatctg gcttgtgata gaatgttcga atcgagaaag aaaacgggag
    10141 taaaaacgat aaccattaga agaaagcttg cccgaacgat aactttcatc caatccatcc
    10201 cgttcgatcg acaaaatcag tgcttgtatt tcttgtactt cttgcttttc atcatcggtt
    10261 aaatcttcca agtacagatc acgtatccgt tcttgataat tttctatcac tgcttcagtg
    10321 gctaaaaatt cttttgtatt acgatcaatt tcctttattt cttttcgcaa aacttcaacg
    10381 acctcttcta aaatcatcaa agcattcaaa tccgtgcttt tcgcttcctc tgtttccgtc
    10441 agcataggca agacaatgat ccctatgacc aaagtcacta agatgacacc cgccgtcaga
    10501 aacaaaagca aagaccgctg gtaaaacatc atattattga ttgaaaatgg caaaataaaa
    10561 atcgtagcca aactgactgt tccctttaca ccgccaaatg ttaagatcaa gatctcgttc
    10621 aactgttttt tgaacttttt actgccatct ttgaatacat aaaaaagact gatagaaatg
    10681 aatctgatca caaaaagcat gacagagatt aatacgataa tcgctaataa aagaccgttt
    10741 ggatataaac cgtctcccca tactggcgag aacacttgcg tcaattcgat tcctaaaaat
    10801 ataaatacta atgcattcag tgtaaaagca atagttgtcc atgtactgtg ggacaagctc
    10861 gacaattctg catcaaacac agagattttc ctaaaaccag aagcttggag aatacctgct
    10921 gccactgcag caatgattcc agatacatca aatgcctctg ccaatacata ggctagaaaa
    10981 ggaagcaaaa gttcgatcag cagataccct gtgacatctt gtgctgatgc tttttcgatc
    11041 aaatgaatta ttttttgttt gaaccagaca agcaaaaagc cgatcaaagc tcccccaata
    11101 ctagaaacta ctagactcat ccccgcgttt actgctgaaa aagatccggt gatcagcgca
    11161 ccaagagcaa actggaaggc agtaacacca gatgcatcat tcaaaagacc ttctccttcc
    11221 aaaatatgca tcgccttttt agggatattc actcgtccag aaagagaact gactgctacc
    11281 gcatctgtcg gacctaacgc tgctccaaaa gcaaagcagg cagcaagtgg gacacttggc
    11341 aacaggaatg ataacgtcgc acctaccccg acaagggtca aaatgactcc tccaaatgcc
    11401 aagaacaaaa ttgtcccgaa atttttgaga atcgaagaaa tatctgcttt ttctccttct
    11461 ctaaataaca atggtgcaat aatcataact aagaacaatt caggttcaaa atcgattgat
    11521 tgtccccact ctgtcagacc taataccact ccaaggaaaa tttggatcag tggagctgga
    11581 atcattggca aaatgcggct agctatgttc gataaggtga tcgttactgc aaatacaata
    11641 actaactcag caaattccat cgtccatccc ctttattttt tattttgtgt aagcattttt
    11701 aaaaattgtt cttttttctt taaaattttg gtttcgacta cttcaacctg tgtttggttc
    11761 ccctctaatt cacactgtac acgttttgac tccaaacgaa gcagatcatg tttcagacgt
    11821 tccaactgac gatacatcaa tatatttctt ttacgcaact tacaaagtat ttgacggcgg
    11881 tcatccatcg aaagggcgcc atagcgatca tgcaacagtt ttttttgttg ttcaaaactc
    11941 tctctcattt tccagatctc ctatacacaa acttccatca ttatagcaaa gttctttttg
    12001 gatgaaaaat atttcattgt ttcatcgttt attgctagtt agaaatttcc ataaattttt
    12061 caacgcctca ttctattcca aatagtatca gatatcaagt aaaacttaag aaagataaaa
    12121 gtttctgaca aaaggatgtt tactttccca ttcctgcctt tcttgttctt ttatcacaac
    12181 aaaaaaatgg ggctgcgaca aaaatcttgt cacagtccct tattctgaat aaacggtgtg
    12241 gaagagacag cctatcagta gtaataagac acatctcttt cacagcctca tttttgcgta
    12301 aaaatcgatt tattcgaaaa attccccgta tacatcttct acacgttgat ctggattgat
    12361 cacgatatac aagttacctg ttttttcgct gatttttgtt gattgatatt gattgtctaa
    12421 tccttctgtt cctttatcta gatatgggaa gtacacaaga tcaggtgtgc catttcggta
    12481 aaggatttcc attcgctcga tatcttcgta tttcaatgcg cgattaaaca tgcccatttc
    12541 cagtccgcct aggttgatat catgtgtcgc aactgcgtcc ccttcatgga agatttcgat
    12601 tttaaatcca gcacacggat ggatttcaac aaattcactg ccattgattc gtccaaaact
    12661 tgttgtgatt tgtttgatcc aaagatcgcc gatatgacgg cggttgatcg tccaggtttc
    12721 tccatttcta aaataaaatt ttagcgctgt catttcttta ctcattaaaa agactccttt
    12781 atgatgtaat ttattcttgt actacctcag tttagcacaa atttatgtaa gtggtcacat
    12841 cttatgtttt ttcttctaat gacgtatctg cccactttaa gtagccaaca aagctgagca
    12901 gatacagtct agtgtttttt aaatcaattt ttctttcgat aaaaaatcca aaacctgttt
    12961 tttatcattt tttattcttt gttctacgac ggctttttca gctaattgca aagcttcact
    13021 aacccactta ccaggttttt ttccagtttc ttctagcaag attttaccat ctactgctaa
    13081 ttcgcttaat gacttgatag gtaaacgatc ataggattct cctacggcat ccatctgagg
    13141 tgtttcgtca aaatagatca tcaattcttc tacttgcagt gcatgttttt tgcctaagaa
    13201 gtacaattcg aatggttcca aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc
    13261 ttgaacgtct ttaatcattt ggttagaaca cttccacgat ttcaaaaagg aacgaatctc
    13321 attttctttt agatccagtt ggctgatcaa taatgcccat gcttggctct cttctttcaa
    13381 tgggacggat ggtaattcag caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg
    13441 acagcaaatg tagcactctg tttcaataaa tgcatggatt cccgcggccc gttcatttcc
    13501 caagagcatc ttgatgaatt ccacatttat acgttctaca gatattttac ctaatagctc
    13561 gtggttttct ttgatggcgt agaaggtttc ttcctctaat ttgaacccta gttgactgac
    13621 aaaacgcaac ccgcgcatca ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc
    13681 tacagctcgc aagatctgtt cttctagatc ttgcagacca tcgaacaaat cgatgatttc
    13741 tccatcttct ttcaaagcaa acgcattgat cgtaaaatct cttcttttca aatcttctgc
    13801 caacgaacga acgaattcga catgatctgg tctacggtag tcctgataag ttgattccgt
    13861 ccgaaatgtg gtgatctcat attgttcatc attttctaat accaacacag ttccatgttc
    13921 gatcccaata tctatcgttt ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt
    13981 tgcgatatcc acatcatgga ttggctggtt cagcaagata tcacgaacac ttccacctac
    14041 aaaataagct tcatatcctg cattctctaa ttttttcaga actggaattg ccttttggta
    14101 ttccagaggt aattgtttta atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg
    14161 agataaagac tcagttgcat ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc
    14221 aggaccactt catcttgttt actcgtcctt acgctcaaag cgattatggt ctctttgatt
    14281 gaatttttcc atgttttttt caaaaacttc agttaagtcg atattcaatg aattagccat
    14341 gatcatcgta acgaataaaa catcaccaag ttcttctgct accgagttaa ctggttcaga
    14401 agcctttttg cttttttctc cataagtgtg gttgacttct cttgccaatt cacctacttc
    14461 ttcagttaat cgagccatct gagctaatgg agagaaatac cccaccttga attgctggat
    14521 atattcatcc acttcctgct gcatactgta taaagaacgc tttttatcca tctttttccc
    14581 tccaaaacta tcttaccaaa actttaacgc aaatcctaga taagagcaat tgagaaaatc
    14641 gaatttccat gctacattta ggaaggaatt tctaaatgga aattctgtga aaatacataa
    14701 ttgaaagctg gaattcatat ggtcaaacga aaaacagtaa aagaagttct ttttatcatt
    14761 ataggaacaa gtatttatgc atttggtctt gtttacttga acatcgctaa tcatttagca
    14821 gaagggggag tctcagggat caccttgatc ttaagagctt tatttggtat tgatccagct
    14881 tatacgactt tattaatcaa catcccattg attttactag gaggaaagat cctcggtaaa
    14941 cgttccttgg cctatactgt tctaggtaca ctttctttgt cattcttttt atggatctgg
    15001 caaagggttc cactagaaat caatttacag catgatctac tgatcgtttc tctacttgcg
    15061 ggtttagttg caggtgtagg aagcgggatc gtctaccgca tgggtggaac aacaggcggc
    15121 agtgatatta tcgctcggat tttagaacaa aattacggca tcactatggg gcgttcccta
    15181 ttggcatttg atatcatcgt cttacttgct tcattaacct atatcgactt gaaacgtatg
    15241 atgtatacgt tgattgtttc gtatgttttc agtcgtgtga ttgattcgat cttagatggc
    15301 ggatattctg caaaaggtct acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta
    15361 ctgatgaccg gtctgcaaag aggtgtgact ttcctttctg gagaaggcgc attctctggt
    15421 aattcaaaaa aaatcatcta tatggtcgtt agctctagtg aattaactga agctaagagg
    15481 atcgtccacg agattgacga aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa
    15541 gggcaaggct ttacttattt gaagccacag acaagattac tgaaaaaact ataagaaaaa
    15601 agacaaagat aggctatgag gaaagattcc acatccagct gacctttgtc ttttttagta
    15661 ctatactatt tttaactaat tgttatgttc acttacccgg ttacttttct tttgtgtctg
    15721 caaaaggatc tttcggttaa cttcatcaga taatttcaaa aaagcatccc tatctccatc
    15781 ttctagtgct tgatcgattt gagcataaag catttttagt tttgcctctt cttctttttc
    15841 ttgaaaatat cgttcaatta attcagtatc ttcttcactg actgaagaat cccaagaaga
    15901 aaatggattg tcttctaaga tagatagata ttctcttgtc tgccaagcat tttcgaatat
    15961 acattccaca tataaaggct ttttccaatt caaacggatt tcatgaaaaa tctggtctgt
    16021 gtccgtaaat tcttttccct gcaaaaacaa tttcatgggt tccccttcaa cagatagatc
    16081 actgatctgc agtcctcgat ccgcaaaatt cgccccttca acaaagtgga cattgcttaa
    16141 aatcgcttca tgattggcaa gataattcaa gatccaaaaa acttctcttc ggctaaaaga
    16201 tacatgattg accatccacg ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt
    16261 tctcacctct ctgttaggtc atctaataat gacagtgcct ccatatctcc tggttcaagt
    16321 gttaaataat aagtcaataa ttcttgcgct cttttcagct gcccttcttc tcttaagaaa
    16381 aaggcatact ctttcaagaa atctggttca tgtgatagtt catgataagc ctgttcatag
    16441 tgtacagctg caacggtaaa gtcttctaat tcattataag catgggctag gttccattct
    16501 gcataaggat tagcagtttc ttccatctga ttgatcactt gtatcacatc ttcatatctt
    16561 tcatcatcca agtaaagatt acttaaagta agtaatgttt catcttgttt gtctcctact
    16621 tctaaagctt tcagtaaaag ttcttctgct cgctgtctat catgcagacg gaaagcattt
    16681 tccgaggcca ggtgataaag ttcgacttgg taaggatttt ctttgattcc ttcttctaac
    16741 actgtttgtg cttcttcaat catttcttct tcttgaagag cttgtcctag atatagatat
    16801 aacgattgat aatgcggatt aacttctcga agttcttcca gatagttgat tgcctgctga
    16861 ttttctttca attgaagatt cgtaaaagct agctggaaca gtcgatcatc tgtcctttct
    16921 tctttcagtg cttcttctaa aggtacgata gctgcttcga attctccttg catacttaag
    16981 gattgtccta aacgttcctg catcgagatt cctgaaattt catttatccc gcttgccaac
    17041 aatgtttcat aaaccactgc tgcctctttg aaacgatcaa ctgaaaaata caattcagct
    17101 agcgcaaact ggatcaatgg ttcatcagga agcaacccag ctgcttcttt caattttgct
    17161 tcactcactt cagggattcc gataacttgg tacaaatcag ctatcgccaa taagctttgc
    17221 ggatagtaat cactttgctt atccactttt tccaggtatt caaaagctaa gtcgatctca
    17281 ttatcttcga ttgcaatttc tgctaacggg atgttcagtc cgtcattttc tgggtattgg
    17341 ttgatcagtt gttcaaaaat ctgttttgct tcgatcaaaa agccaatagc cagcaattct
    17401 tcgcctagtt cagctaatat atctgctggg tcttctttta atgctttttc taaaaagatt
    17461 tgtgcttgtg acaagtcttc atctgataaa gcttgcaaca ttttttcgct atttgtattc
    17521 atttcaattc cctcttatct cactgacgat ttgttgaaat accgtcattg ttctcaataa
    17581 ttctttttcg tttggatgtt ggtatccgtc tattatacct attttttcta aaacaagtat
    17641 ttttttgctt ttttctgcca aaaaaagaac tttttccaaa tactccatct ttgaaatctg
    17701 ttccagccag tggattggat agccaagcaa gtccagccac acaaaaaagt tttttagttg
    17761 gaaatcaaaa ggatatgctt ggagattcca cacgaaatga aacatcattc ctaaaaattt
    17821 tttcatacta gcagaaagta gatggccatt ttcggtcaga taaaaagctt gttcaaacag
    17881 tttaccataa tttgacaggt tttctgcctc taattgataa aaatagatca tttcttcgat
    17941 catcccggca aacggccgag tcgttaattg ctttttattt ggatagtttt tatacagatt
    18001 ttgcaaaaaa cgatgatcac aaatcattcc gcaacagata aaagtcaaaa agtcaatctg
    18061 ctttccgtcc acctgccgct cgctgattgt ttgatcataa acgatgcgtt ctggcagatt
    18121 gatggtttgc agcactacac gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc
    18181 tcggcttaga gaacggatcg atacagggat catccatagt gatgaatcaa gggttgtatg
    18241 tttttgaaaa aaacctgcca gatccatgat cccttcattt ccaaatccga tgataaacat
    18301 ggattgattt tcgggatagc gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg
    18361 attgcattga gtgttggcac aaatatacca gtcattgttt gcttgatttg caaataacgg
    18421 gttgatctta tctgcaaaca aatcataata gcgctgattc gttaaaaata agattggctg
    18481 atcaatggaa agttctctta gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa
    18541 cgcatcttct acttgataat caagttccat acgcatccct cctcttctta tttttatttt
    18601 accattagta agcaaattct agaaaagata agagcaaagc tcacctcata acttgcaaat
    18661 aaataaaaat taaataaaat acatgtgttt tctatgtatg acatacggat tcggaatctc
    18721 tgtctcttat ttattccttt tctgctagga aataaaaaga aaagcacttg tagccctaga
    18781 gccacaagcg ctttttcaac tgtcttattt aacagcgtct tttaatgctt tacctggttt
    18841 gaatgcaggt actttgctag ctggaatttc gatttcttca ccagtttgtg ggttacgacc
    18901 tttgcgagct gcgcgttcgc gaacttcaaa gttaccaaaa ccgattaatt gaactttttc
    18961 gccgttagct aaagcatctt gaatagttga aaatacagca tctactgctg cagttgcatc
    19021 ttttttagtt aaatcagtag ctgaagcaac tttttcgatt aattctgctt tgtttgccat
    19081 ggatagattc acctcctcca caagagatat gatagaaata tcatttagtt aaaactttct
    19141 gagtggtcca gaagtttaaa aagaaatatt gattaacttc aatattctgt tatcacgata
    19201 tcatatgaac cgcttaatag caaggttttt accaattttc acatgctttt tttagtcata
    19261 gaaacgtaac aaacgaaact gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat
    19321 gcctattttc tcctacgagg gataattcgg atggacgtcc cttcaaaagt aaatgctttt
    19381 ctgatttgat tttctaagaa acgttcataa gaaaaatgca tcaattcctc ttcattgaca
    19441 aaaacgacaa atgtaggtgg tttgacagcg acttgtgtag cataaaaaat cttcaggcgt
    19501 tttcctttat ctgttggtgt tggattgatt gctacagcat ccataatgat atcattcaac
    19561 acagccgaag agatacgaag attctggttc atacttactt gttcgatcat ttcaggcaga
    19621 cgttccaacc gttgtttagt cactgcagaa acaaaaacga ttggagcgta atcaagatac
    19681 cggaattcat cacgaatctc ttgttcgaaa tcacgcattg tattggtttc tttctttaca
    19741 agatcccatt tattcacgac gataataatt ccacgaccag cttcatgggc gtaacccgct
    19801 actcgcttgt cttgttcccg aatcccttct tcagcattca gtaccatcaa aacgatatca
    19861 gaacggtcaa tggcacgcat tgcacgcatc acgctgtatt tttcagtatt ttcgtatact
    19921 tttccgcgtt tacgcatacc agctgtatct atcattaaga atttctgacc actttcagaa
    19981 acgaagtggg tgtcgattgc gtctcgggtc gttccttcaa tatccgaaac aattacacgt
    20041 tcttctccta aaatcgcatt gatcaaagag gatttaccaa catttgggcg accaatcaaa
    20101 ctaaacttaa tcgtgtcgtc gtcctcttct tcagtctctt cagagaaata cttcactgct
    20161 tcatctaaaa tatcaccaat ccctagtcca tggcttcctg aaacaggata aggatcgcct
    20221 agccctaagg aataaaattc atagatatcg tttctcattt ctggattatc tactttattg
    20281 actgctaaaa tcactggttt attacttctg tataaaattt tcgcaaccat ctcatctgcg
    20341 tctgtcacgc cttcgcgtcc gctgactaca caaatgatta catctgcttc ttcaatcgcg
    20401 atttctgctt gatgcttgat ctgatccata aaaggttcat cgccaagatc aattccgcct
    20461 gtgtcgatca cgctgaattc tcgacctaac cattcacctt ttgcataaat acgatcacga
    20521 gttactccag gagtatcttc gacaatcgag atacgctcac cagcgatgcg gttaaaaatc
    20581 gtagacttac cgacgttcgg gcggccaacg attgcaattg ttggatttgc catattattt
    20641 cctcctcaca agtttccaat ctcacttagt ttaacgaatg cattgccaac tttcaagcat
    20701 agattgtcaa ttaaacgaag tatttttatt ccttattgaa atactaatcg cttttcaaaa
    20761 gaaaaactga gacaaaagca gctgctttca tctcagtcac agatacaaaa tcaattattt
    20821 ttcagaatca tcggttgctt tttcttctac ttcttctttc aacgcatcac ctaaaatgtc
    20881 tcccatagta aatccagtat tttcttctgg taattcataa gactcaacat tttttggttc
    20941 ttcttctgaa gctggttttt cttctaaagc tttgatactc aatgcaatac gatgttcttc
    21001 tggatggatc tctaatactt tcactttcac ttcgtcacct tcatgaagca cttcatgtgg
    21061 tgtcgcaata tgtttatgtg aaatttgaga gatatggact agcccttcta ctccagggaa
    21121 cacttcaaca aaggcaccga agcttgtcaa acgtttaact gttccgtcta atactgtgcc
    21181 aactggagct tttgtatcga tttctgacca aggtccaggc tgtgtgtctt tgattgacaa
    21241 ggaaacacgt tcttgttcag gattgataga caacactttg acatttacat catcattgac
    21301 agccaataca tcacttggtt ttgctacatg actatgtgaa atctctgaga catggactag
    21361 accgtcaatt ccgcctaggt cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc
    21421 actgagaaca tctccctcat ggatagttga aagaatttct tttttctttt cttctctttc
    21481 gttttccact accgctttat gcgataagat caaacgattt tctgatggtt cgatttcgat
    21541 gattttaaag ctcaatgttt tgcctttata gtctgaaaag tctgcaacaa aatgatcttc
    21601 caccattgat gcagggacaa aaccacgtac accgacgtca acgactaaac cgcctttgac
    21661 aacatttgta acaggtgctt cgatgacctt gccctcttgg aaatctcttt cgatatcttc
    21721 ccaaactttt ttagcatcta aacggcgttt tgaaagtaga tagctgccgt tttctttatc
    21781 tttgccgatc gtactgataa cgactaaatc taatacatca ccgactttta ctacttcgct
    21841 gatatcttct accggcaaag ttgataattc ttttgccgga acgactcctt caactccagc
    21901 cccttcgatt ccgacaatca cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac
    21961 atcgccaact tttacttctt gtacgctttt cattgcgtct tccattgact ggttgttctc
    22021 ttgattttga tcaaattctg tcataacact tgtcctccta accaacattc tgccgtaagt
    22081 acggtactgt ttttagttta attgattatt caaaaaaaat aaagcgattt cgttcgtttt
    22141 ccttgaaaaa acaattgttt ttcatttttt ttcttaattt aaaaaagaag ataacctttt
    22201 ttcaaaacaa cagcttttat tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg
    22261 atacgaacag catcctctgc ttgcttcagc ggagagacgt cccttgttga atcataatgg
    22321 tctctttcct gtatctcttt cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg
    22381 ttctctttga aacgtctttc tgcacgctca cttacgcttg ctactaaaaa gattttcact
    22441 tctgcattcg gtaatacagc tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt
    22501 tcccctattt ttcgttggac agcaactaat tcttctctga cttttccatg tgcagagact
    22561 tcagaaacag cttttgtgac atctggttga cggatttcta aagtcacatc ttttgatgct
    22621 atgaatacat gctgtccatc ttcttgttgc gaaaaagaga taggatactt tttgatcagc
    22681 gaaactagcc cttcttcatc tgaaaaagat acattgttcg taatagctaa ataagttact
    22741 gcgcgataca tggcaccggt atctgtataa atataattca attcttttgc taatatcttg
    22801 gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg
    22861 atataactcc tttttctcag aaaaaaagcc gcaaagcgcg gctattatta tttaattctt
    22921 aattcttgac cagggtaaag cataaagttg tttggatcta agccattcaa ttcaaacaat
    22981 tgatcagtgg tgatccctgc acgctcagcc acttgttttg gaccttcgcc tgcttgaaca
    23041 gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg tttctgctgc attgctgctt
    23101 gaatcccctg cttgataagt tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata
    23161 ctgctttctg acgttacacc tgtatcttga gacacagtag attcttccgc gattgtacta
    23221 gattctactg ttgaagtctc agttgactcc tcttttgttg tacttgtaga actttctact
    23281 actgatgtcg aagtttcaga agaagttgct gctgttgcat tgttgcttga tccatttctg
    23341 atccagaaat aagcacctgc tggaatagcg atacataaga ccagtaaaat caaaaatagc
    23401 gaaagaaata atgtatttcc tttcttttgt tgtctctgag aacttcttga tgaaacttca
    23461 tcatctgtat cgtagattgg ttgttcccat ggttcttgtg tttcgttatt ttctgtagat
    23521 tgatgtctat cttttctgct cactgtttta cctcctaa
//
LOCUS       DASJON010000023        23506 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJON010000023 DASJON010000000
VERSION     DASJON010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.023"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            350..547
                     /locus_tag="U3989_000699"
     CDS             350..547
                     /locus_tag="U3989_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN1714167.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            549..860
                     /locus_tag="U3989_000700"
     CDS             549..860
                     /locus_tag="U3989_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1714168.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            964..1110
                     /locus_tag="U3989_000701"
     CDS             964..1110
                     /locus_tag="U3989_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN1714169.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            1186..2433
                     /locus_tag="U3989_000702"
     CDS             1186..2433
                     /locus_tag="U3989_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1714170.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            2448..3200
                     /locus_tag="U3989_000703"
     CDS             2448..3200
                     /locus_tag="U3989_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1714171.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(3903..4118)
                     /locus_tag="U3989_000704"
     CDS             complement(3903..4118)
                     /locus_tag="U3989_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEN1714172.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(4137..4373)
                     /locus_tag="U3989_000705"
     CDS             complement(4137..4373)
                     /locus_tag="U3989_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEN1714173.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            4631..6784
                     /locus_tag="U3989_000706"
     CDS             4631..6784
                     /locus_tag="U3989_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEN1714174.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            6753..8168
                     /locus_tag="U3989_000707"
     CDS             6753..8168
                     /locus_tag="U3989_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN1714175.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            8201..9235
                     /gene="sppA"
                     /locus_tag="U3989_000708"
     CDS             8201..9235
                     /gene="sppA"
                     /locus_tag="U3989_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1714176.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            9237..9758
                     /locus_tag="U3989_000709"
     CDS             9237..9758
                     /locus_tag="U3989_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1714177.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            9755..10141
                     /locus_tag="U3989_000710"
     CDS             9755..10141
                     /locus_tag="U3989_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1714178.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(10637..10709)
                     /locus_tag="U3989_000711"
     tRNA            complement(10637..10709)
                     /locus_tag="U3989_000711"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10674..10676),aa:Lys,seq:ctt)
     gene            11090..11659
                     /locus_tag="U3989_000712"
     CDS             11090..11659
                     /locus_tag="U3989_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN1714179.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            11646..12506
                     /locus_tag="U3989_000713"
     CDS             11646..12506
                     /locus_tag="U3989_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1714180.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            12696..13400
                     /locus_tag="U3989_000714"
     CDS             12696..13400
                     /locus_tag="U3989_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1714181.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            13405..15240
                     /gene="walK"
                     /locus_tag="U3989_000715"
     CDS             13405..15240
                     /gene="walK"
                     /locus_tag="U3989_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN1714182.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            15237..16553
                     /locus_tag="U3989_000716"
     CDS             15237..16553
                     /locus_tag="U3989_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714183.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            16554..17423
                     /locus_tag="U3989_000717"
     CDS             16554..17423
                     /locus_tag="U3989_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN1714184.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            17478..18287
                     /locus_tag="U3989_000718"
     CDS             17478..18287
                     /locus_tag="U3989_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1714185.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(18390..19019)
                     /locus_tag="U3989_000719"
     CDS             complement(18390..19019)
                     /locus_tag="U3989_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN1714186.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            19189..20481
                     /locus_tag="U3989_000720"
     CDS             19189..20481
                     /locus_tag="U3989_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1714187.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(20557..21219)
                     /locus_tag="U3989_000721"
     CDS             complement(20557..21219)
                     /locus_tag="U3989_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1714188.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            21444..21728
                     /gene="groES"
                     /locus_tag="U3989_000722"
     CDS             21444..21728
                     /gene="groES"
                     /locus_tag="U3989_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN1714189.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            21779..23404
                     /gene="groL"
                     /locus_tag="U3989_000723"
     CDS             21779..23404
                     /gene="groL"
                     /locus_tag="U3989_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN1714190.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         8099 a         3685 c         4658 g         7064 t
ORIGIN      
        1 tatccaaatt tggaagctcc atgttcgtct ctatcttttt tgtctataaa aaaagagaat
       61 gcgtagctca tcttcgaaac gtccaaagac gccgacaaac tattatgaat ttatgggtgc
      121 gagtcaaaga gatcgatcgt aggtatgaaa gactaatgtg attaaagttt attatgtacg
      181 aagtgcattc tcaaaaaata ttaaagaata tacatagttt atatacttaa aagtgattaa
      241 attcttattc aggaattatt ttgctacgtt tcaagaagga attgtttttt gaaatttaat
      301 gaatgttaga ataatagtct aaaaacatta atttaggggt gattagatta tgaaacattt
      361 aaaaattttg tctattaaag agacacaact tatctatggg ggtaccactc atagtggaaa
      421 atattatgga aatggagtgt attgcactaa aaataaatgt acggtcgatt gggccaaggc
      481 aactacttgt attgcaggaa tgtctatagg tggtttttta ggtggagcaa ttccagggaa
      541 gtgctaaaat gaaaaaaaat gctaagcaaa ttgttcatga attatataat gatatatcta
      601 taagtaaaga tcctaaatat tctgatattc ttgaggtttt acaaaaggta tatttaaaat
      661 tagaaaaaca aaaatatgaa ttagatcccg gtcctttaat aaatagattg gtgaattatc
      721 tatattttac tgcttatact aataaaataa gattcactga atatcaagag gaattaataa
      781 gaaatttgag tgaaattgga agaactgctg gaataaatgg tttatatcga gcagattatg
      841 gagataaatc tcaattttaa tgatacttat aatattaaat atgtctcaaa attttttgat
      901 tagaaaaata ggtatattga atcagtgaca taaaagtatt tatagtttgt agggggaatc
      961 gtaatggaag aaaaaaatag actaaatgct aaacaatgtt cagatcaaga gcttaaaaaa
     1021 attaaaggag gtgctggaac aaaacctcaa ggaaaaccgg catcaaattt agtggaatgt
     1081 gttttttcat tatttaaaaa gtgtaactaa aatttttttt atgatatcct aaaatcagga
     1141 ggggatctct tgattactat cgatacaaaa gcttatctat taagaatgtt cgtgggaaac
     1201 tttttctttt ttctcttatt ttatatgact aatactgatc ttaacaaaag aaaatatctt
     1261 tttgtatgtt tattgatacc actcactttt tttgttgaat tatttactga tgtttccgca
     1321 gctataccta taatcggaag ttattttata ttaaaggaaa agaagaaaac taatatagtg
     1381 cttttaaata aattactatt atgtatgctt attgatgatt tcagatctat tattacttcc
     1441 atggttatga cgtatatgtt ttcatttgaa gggataaaag ggtatggtta tgtagtattc
     1501 cagataatgc ttgattcttt gtttttgctg atgtttattt ggttatatag aaaattagct
     1561 ataaatagga ttgttgaaca gtccagttct aaacttactg ctatattcat gatttattta
     1621 cttacaattg gtttatttgt ttcttatgtt gctcatcaat atcaagtatt tgatcatttt
     1681 gtgtttggtg tcttaatatt tttaatcatt caaatgtttg ttgttctatt tttatttatt
     1741 agaataacta ttaggcaacg agaacaatat gaacagcaat tgaaaagaaa agaattggtt
     1801 tatctaaaaa aatatactga ttcactggaa aaagatcaag aaaaatttgc cagatttaga
     1861 catgactata aaaatttatt gctgagttta aaagaaatcg ctaatcaaaa ccatgattcg
     1921 atattggaaa aagaaataaa agaattagag atctattcta attcttatct aaccaataag
     1981 tttgaatatc gttatttaaa aaatataaaa aatgaatatt tgaaaagttt gttgatagcg
     2041 aagctatacc aagcaaataa caataagatt tgttgtaaat tcgaatgtcc aagtactatt
     2101 attgaagttc ctgttccaat atttgactgt attagggtgc ttggaattat tctcgataat
     2161 gcaattgaag catctataga gagtcaagat agagaactgt ctctagctat ttaccgagat
     2221 gataaacaga tagaaatttc gatatcgaat agttgtagag agatactact ctctatagat
     2281 actctaatga aaaaaggaat aacaacaaaa aagggacatc aagggttagg tttatctagt
     2341 atagaggaaa tcaataaaaa gaatcccaat atgtttgtga actatcaaaa cgatccgtat
     2401 caatttacta ctcaagttat tttgttgttt tgagggaagg aggatttatg agttatccaa
     2461 taatattatg tgaagatcaa attatacaat taaatcagtt agaacgaatt attgataatt
     2521 tcattctttt tcacgataaa gtatttaaga tagtattgaa aacacaaagt ccgttagaag
     2581 ttaaaaaata cctcaaacaa ttccgaccaa agcaaggaat atatttctta gatattgatt
     2641 taaatcatga agttaacggt atagaattag cagaagtaat cagaaaatat gatgttcaag
     2701 caaaaatcat ttttacaact actcatgatg agatgttacc cgtaacaata aaaagaagag
     2761 ttgaaacgtt aggatttgta acaaaagatc aaacactaga tgagtatcga aacgagattg
     2821 ttgagttatt gttattagcg caagaaagga tagatgcaac aaaacaggtt acgaatcaag
     2881 catttgtttt ttccataggt tctcaaacat ttacttttga tataaatgac atttattttg
     2941 tagaagcttc aaaattacca catagattat cattgtgtac taaagatggg cagtacgaat
     3001 tttatggaag aattagtgag cttgaaaaga aatatcctat gttaacaaga attagtcgag
     3061 cgtgtttagt aaatatcttt aacgttaaag aaatagattt taaaaaacgt tcactatatt
     3121 ttgattcaga gttggccaga aactttactt tagggaaagc acaaaaaatt aaggagaagt
     3181 tgaaagaaag cactgtttag tggttttttc tttagggaga ttgcattttt ctaataaaat
     3241 agattccttc actagtgcaa atttttctga atcctattaa agttgtacca atttttatag
     3301 ctgttattag ttacgtgcat aacagcaata ttagttactg gaggaatatt tctatggtct
     3361 ggaaccaaaa aagggccaaa tgaatagttc ttttcttgtt tttattaagt accataaagg
     3421 ggaattaaaa ttaaatgtat ggtggaagat gcgaaagatt tggcaaattc tactaatttt
     3481 tagaaagtgt ttttcatttt ttagttaaaa aatttgtcct gtcaagtgca atcctatgat
     3541 tgtacttgat aggatttgag ataattactt tcctgttttt ataaatttaa cttgcaggaa
     3601 caggctttaa tgcacatccg acaagtacat taccaatacc tcctgtaacg gccgaaatag
     3661 gtcctcctgc agcaccaact acagcgctgc aagtcctttt gtaaaaatat ctctaccatt
     3721 acatttccaa agaattttta tctgacttta ataaaagata aaaatttaat ataattataa
     3781 aaactagcac aagaagcttt agtaaaaagt tctgtccatt ccaaactttt gaaaagataa
     3841 agaacatacc tatgttcgta atcgacacaa accaatcttg aggtttgata ttttttaatt
     3901 gtttacgcac cggtccacat gctgaatcca gcacctaata aagctccacc tacaccacaa
     3961 gcagctattg cataaggtcc tccggctgaa caaagtttta ctcctgctgt tgccccagaa
     4021 aaagtagtgc ctaaaactcc cacggcactc ccaccttgaa tatttttcaa ctcttctgaa
     4081 gataatataa atgttgtttt tgtgttttta atatgcatac tacttattct ccttttttat
     4141 ttttttcgag catcccagcc agctgcagca cctccagcga cagctccata taaccctcca
     4201 acaacaccgc aagctggtcc gcaaattgct gttcctaaac ctgcaccacc tactgcccct
     4261 gtggtaagtc cacctacaac ttgccccaat tttccccaag cattattgcc tccaccaaca
     4321 gtttgtgcat ttccacaata cttagttcct ttacattttg catgtttaaa cacgcttctt
     4381 taaataatat tttttacggt gctactgtaa catacgtatt tgattaacag taaaaatctt
     4441 cctgaaactt atcaagttta ttcttgaata gtaaaatttc caaaataaat ttgattcagg
     4501 aatgaaaaag ttagtgttca agaactgttt tcaaagtttt tgtggaatta tgagacaata
     4561 ttttcactgt attaaagcta tattgagaat ttttctataa aaatttttta gaaggagttg
     4621 tgttagataa gtgataaaat catttccttt tgtgcagcaa caagatgaaa aagattgtgg
     4681 tgttgcttgc ctttcgatga ttttaaaata ttttaaaact gatatcccga ttcataaatt
     4741 acgggaccta tctgggactg atttagaggg tacttcagca tttggattga aaaagacatt
     4801 tgaaaaattg tcttttgatt gtatggctat tcaagcagat agtgagatct ggaaagaaag
     4861 agatcttatc ttaccattaa ttgcacatat attggttgat aacagctata tgcactacgt
     4921 tgtaatttat aaaacgaagg gaaacgtact atggattgca gatcccgcta atggaaaaaa
     4981 tagaaaaacg attgaagcgt ttcaaaaaga atggacaggt attcttttat tacctatgcc
     5041 tagagaaact tatattccta ttaaagaaaa agtagctgga ttaagttctt ttttgcctat
     5101 aatttggaaa caaaaaggat tagtattcca catcgtttta gcatcgattt tcattacgtt
     5161 ttttggcata ggaagctcat attattttca aggtattctt gattttttca tccctaatca
     5221 agcacgttca actttaaata taatttccat tggcttgatc gctgtctatt tatttcgggt
     5281 attgtttgaa tatagtcgta gttatcttct tattatttta ggacagcgaa tgagtatggc
     5341 aattatgctt cagtatttca aacatgtgtt aactttacca atgaattttt ttgctacgag
     5401 aaaatcaggg gaaattattt ctcgttttct tgatgccagt aagattattg atgctttagc
     5461 aagtgctacg ttatcaatat ttttagatat tggtatggtg ttgcttgttg gggttacgtt
     5521 agctattcag aatagtacgt tgtttttaat cactctatgc tctttgcctt tttatgtagt
     5581 tgcaatattc gctttcgtga aaagctatga aaaatcgaac agggaagaaa tggcagccgg
     5641 tgcagctgta aattctagta tcatagaaag tcttaaagga atagaaacaa ttaaagcata
     5701 tagcggtgaa gagaaagtat ataaccgtat agaccatgaa tttgttaaat taatgaaaaa
     5761 atcatttcgt acggttacgc ttgataatat tcaacaagga attaaacatt tgattcaatt
     5821 aattagcagt gcattaattt tatggctagg ttcttatttt gttatggata atagaattag
     5881 tctaggtcaa cttatcacat acaatgcttt actagtattt ttgacagatc ctttacaaaa
     5941 cattattaat ttacaagtga aaatgcaaac ggctcaagtt gcaaataatc gtttaaatga
     6001 aattttcgcg attgattcag aaaaatcgaa ccagtctttt gaatatatgc tttctgaaca
     6061 attatttcaa caagatatca ctgtaaaaaa cgtttcattc tcgtatagca tgaaggcccc
     6121 aacgttgaac gaaatttcat gtacaattcc ttcgcacagt aaaacggcta tagttggagt
     6181 tagtggttct ggaaaatcaa ctcttgcaaa gttatttgtt cgtttttatg aagcatcaga
     6241 aggatcgatc cattatggaa acattaatca cttagacatt ccttttcaga tattacgcga
     6301 tcgagtgaca tatttgcctc aagagtcatt tttctttagt ggaacaatca ttgagaatct
     6361 tatattcggt ttagatagcc caccaagttt cgaacaaata atagaagttt gtgagatagt
     6421 ccaactaaat gaatttatta atcaacagcc attacgttac gagacattgt tggaagaagg
     6481 tggaaataat ctttcaggag ggcaacgaca acgattagcc ttagcacggg ctttattaaa
     6541 aaatccggac atattaattt tagatgaagc aactagtgga ttagatacct tgttagaaca
     6601 tgcaattatg aattatctcc tcaatttaaa agaaaaaacg attattttta ttgcgcatca
     6661 tttaccaata gcaaaagcat gtgatcaaat tttagttctc catgaagggc atttggtaga
     6721 aaaaggaacg cacgatgaac ttcgttttgc cgatggtatc tatcaaaaat tatgggaaat
     6781 ctaggagagg gaaacaattt atgcagaata ataattggtt cgaacattct agtatctata
     6841 ctaaacaaca tcatagcttc taccgttggg tgttataccc tgtaattctt tttttatgta
     6901 ttattggatt gtttttaacc tttgctaaaa aagaggttgt tattcggaca acggctcaat
     6961 tgaaccccga aaaaatagag aagttacaag tgccattaga agctaaaata atggaaaact
     7021 tcctaaaaga aaatcaattt gttcataagg acgatgtgtt ggttagattg gattgttctt
     7081 taatagagaa tgaaaaggct cagatagaac aagaaaacca acgtattact caacaaataa
     7141 agatggctca gctatttatt gaaagtataa gtaaaggaaa aaatttgttt tcaacggatg
     7201 acagttttgg ctacagtaat caattaaaga gcatgttgtc agaaaaagaa tcactccgct
     7261 acgctttgaa gcaaagtgaa ttaaatgatc aaaagcaatt agaagtatac gaaaagacaa
     7321 aaagacaact agaaaaacaa attgagagtt cagatagtaa attacaagaa tggcaacaag
     7381 tacaggtagc ttggagtaat aatcaatcat taaaagattt ttcaaaagaa atgatggcaa
     7441 actatgagaa ttggcaagaa caactaaata atgtttctga tgatcaaaaa aatcaagtga
     7501 aactgacaat ttcagcaagc ataaatgaac aaattgagca actaaaaaaa gaagtagaac
     7561 agtatcagtc agaaaaagct aaattagtta aaccaactac ttctgagaat gacagaatta
     7621 gtcaaacgga aaaaggaaag caagagctag aacaagcaat aacaactgta aaacaaacag
     7681 ttacagagct aaatgaaaaa caagagaaga accagtcagt tatcaaaagt ttggacgatc
     7741 aactttcaaa aggaatacta aaagctcctg tatctggaac ggttcattta aatgaagaaa
     7801 caaagggaca agcagaacta gccaaaggaa cagtgcttgc agaaatttat ccgatccacg
     7861 aaaaatcaaa aatgaaattt acagcacttt tacctgcgaa tgaaagtacc tatattaaag
     7921 aagggatgaa ggttcatttt aaattggatc aaaaaggtgg ggctccgatt acgatcgacg
     7981 gaactttaga tgaaatttca gaaaattcta ctgccactga gaaaggtgtt ttttatgcag
     8041 tgaaaggaac gttaaaaaca actcaatcgt ttccgtttcg ttatggatta actggagagt
     8101 tgtctttaat tgtaggagaa aaaacatatt ggcaacaaat tgtcgatgtg tttctaaata
     8161 aaaaataatt atttttacta agaatatagg aggagtttat atgaataaaa gaagatggat
     8221 tgctgtggga gtagcgtgcg gattattgtt actatccatt atcgtcgcgc taattccagg
     8281 aaaagataaa gaggaagcat caaatacaac cctaacagga ataaataaga ttttttacgg
     8341 atctaacgaa atcacagaag aaacgttaga agaaggcgca tcaaataaaa agattgtcaa
     8401 actatccgtc aatggagtta ttgcagatac aggtgaaagc aatcttttta gtagggaaca
     8461 atataaccat cagaattttt taacacagct aaagaaaatt caagaagaca aagcggttaa
     8521 gggagtgtta ttagaagtaa attcacctgg gggaggaata tatgaaagtg ctgagattgc
     8581 taaagaaatg gcaaaaatta aaaagctaga tattcctatc tatactgcat ttaaaaatac
     8641 agcggcaagt ggagggtact acatttctgc tggttcagat aaaatatttg ctacggaaga
     8701 aacaacaact ggatcaattg gtgtcattat ttctggttta aattattctg gcttattaga
     8761 aaaacttggc gtaactgatg cgacgtataa aagtggtgca ttaaaagata tgatgtcacc
     8821 tcaacacaaa ccttcagagg aagagaataa agttatacag gaatttgtga tgagtgctta
     8881 tgatcgtttt gtcaatgttg tagcaaaagg aagaaatatg gatactaatg cagtgaaaga
     8941 acttgcggat ggaagaattt atgatggtaa ccaagcagta gaaaatgggt tggtggatca
     9001 aattggttac tctgaagatg cattagattc cctaaaaaaa gagaagaaat taactgatgc
     9061 gacggttata gaatataaaa atgacacaac tggctttgct tcttcttggt taggtaataa
     9121 gattgctgaa tggcaagggc taaaggcaac tacatcagat cgtgtaattt cagtttttga
     9181 aaaacttgga ttttctgata ctccaaaacc aatgtattac tatggagggc tataaaatga
     9241 ataagaagag tggcattgaa actttgccta attatgtatt tgcgggattt tggttacgat
     9301 tttttgcttt tcttattgat ttaatttgta ttagcatcat cacccatgct actataggtc
     9361 tcttttacaa aatcatggga ctggaatctg acaaatccat tattactttt tatggtatag
     9421 cgtcattagc tatttattta acctattttg tacttttgac taagctgaat tcaggacaaa
     9481 caattgggaa aatgattttt gggataagag tagtgagctt tgaggaaaaa gagctaagtt
     9541 ggcaaactat ctttattcgt gagtgtatat gtaggtttat attaaaaaat tttgtatttc
     9601 aaattgggta tttggtggct gctttcacgc ccaagaaaca acatattggg gattattttt
     9661 ctaacacgtc agtggttgtt attcgttcat tagaagctat taactatggt caaaaaattt
     9721 ctaattctaa aacacttaat ctggagagaa ttaaatgaat aagagaaaaa cagagtttat
     9781 tttgatttta attagtggaa taacagggat tttatgtagt cttttagcat tgatgggtgg
     9841 tatgtttcta tcttctattg attcaactac tttaaatact acagaactaa ccagtaagga
     9901 tatagatatg ttagtcaatc taagcgggaa tgttgtttta tttggaggaa ttaccctagt
     9961 tttttcgata atgattttca taataggttt tttagtaaga aaaaatcaaa aaacagtggt
    10021 ctatggtatt agtttactca ttttatctat tattccattt tttcttttaa cattattgtg
    10081 ggtaattcct ggagttttag gtattattgc gggattgatg cttctaataa gaaaatctta
    10141 attaggcaat gtgtagacaa cgtgaaacga ttaatcaatt aaaagctaaa tacgttttga
    10201 gaaatgataa cttgtagtcg atggaagaca tgtagataaa aaaactacgt gtcttttatt
    10261 tattctaatt ttttcgaaaa aaagacactc tctgattgtt gtattaatgg aaaaggggaa
    10321 agcagcaagt aaatttagtt tttctattaa atattcttca ctttttaacc ttttatagta
    10381 cttagattta atcctatatg tttagagagt tcattggcag catgattttg caaagcttct
    10441 cgaattattg ttatattaga aactttactg ttcataatta tgcttctttt ttaactcaaa
    10501 gtccaaaggg ctacagtgga ctttgagtta aaaataaaaa gccctagtta ttttgaaaaa
    10561 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
    10621 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
    10681 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
    10741 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
    10801 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
    10861 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
    10921 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
    10981 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
    11041 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
    11101 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
    11161 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
    11221 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
    11281 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
    11341 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
    11401 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
    11461 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
    11521 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
    11581 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
    11641 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gttccagaag
    11701 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
    11761 tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg ccagaggaaa
    11821 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
    11881 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
    11941 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
    12001 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
    12061 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
    12121 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
    12181 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
    12241 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
    12301 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
    12361 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
    12421 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
    12481 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
    12541 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
    12601 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
    12661 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
    12721 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgacgttt
    12781 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
    12841 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
    12901 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
    12961 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
    13021 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
    13081 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
    13141 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
    13201 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
    13261 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
    13321 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
    13381 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
    13441 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
    13501 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
    13561 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
    13621 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
    13681 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
    13741 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
    13801 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
    13861 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
    13921 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
    13981 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
    14041 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
    14101 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
    14161 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
    14221 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
    14281 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
    14341 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
    14401 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
    14461 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
    14521 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
    14581 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
    14641 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
    14701 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
    14761 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
    14821 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
    14881 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
    14941 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt gattttaagt
    15001 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
    15061 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
    15121 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
    15181 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
    15241 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
    15301 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
    15361 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
    15421 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
    15481 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
    15541 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
    15601 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
    15661 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
    15721 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
    15781 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
    15841 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aagaagtata
    15901 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
    15961 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
    16021 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
    16081 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
    16141 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
    16201 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
    16261 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
    16321 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
    16381 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
    16441 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
    16501 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
    16561 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
    16621 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
    16681 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
    16741 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
    16801 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
    16861 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
    16921 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
    16981 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
    17041 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
    17101 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
    17161 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
    17221 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
    17281 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
    17341 aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
    17401 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
    17461 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
    17521 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
    17581 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
    17641 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
    17701 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
    17761 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
    17821 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
    17881 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
    17941 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
    18001 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
    18061 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
    18121 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
    18181 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
    18241 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
    18301 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
    18361 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
    18421 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
    18481 gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
    18541 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
    18601 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
    18661 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
    18721 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
    18781 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
    18841 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
    18901 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
    18961 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
    19021 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
    19081 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
    19141 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
    19201 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
    19261 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
    19321 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
    19381 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
    19441 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
    19501 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
    19561 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
    19621 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
    19681 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
    19741 gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
    19801 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
    19861 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
    19921 gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca agagttgacg
    19981 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
    20041 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
    20101 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
    20161 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
    20221 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
    20281 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
    20341 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
    20401 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
    20461 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
    20521 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    20581 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    20641 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    20701 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    20761 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    20821 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    20881 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    20941 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    21001 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    21061 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    21121 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    21181 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    21241 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    21301 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    21361 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    21421 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    21481 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    21541 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    21601 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    21661 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    21721 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    21781 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    21841 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    21901 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    21961 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    22021 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    22081 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    22141 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    22201 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    22261 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    22321 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    22381 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    22441 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    22501 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    22561 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    22621 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    22681 cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg cttctaaagt
    22741 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    22801 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    22861 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    22921 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    22981 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    23041 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    23101 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    23161 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    23221 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    23281 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    23341 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    23401 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    23461 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacg
//
LOCUS       DASJON010000024        23255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJON010000024 DASJON010000000
VERSION     DASJON010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.024"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..556)
                     /locus_tag="U3989_000724"
     CDS             complement(<1..556)
                     /locus_tag="U3989_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1714191.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTA"
     gene            complement(601..1428)
                     /locus_tag="U3989_000725"
     CDS             complement(601..1428)
                     /locus_tag="U3989_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1714192.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(1425..2492)
                     /locus_tag="U3989_000726"
     CDS             complement(1425..2492)
                     /locus_tag="U3989_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN1714193.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(2518..3354)
                     /locus_tag="U3989_000727"
     CDS             complement(2518..3354)
                     /locus_tag="U3989_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1714194.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(3351..4220)
                     /locus_tag="U3989_000728"
     CDS             complement(3351..4220)
                     /locus_tag="U3989_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1714195.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(4290..5573)
                     /locus_tag="U3989_000729"
     CDS             complement(4290..5573)
                     /locus_tag="U3989_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1714196.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(5587..7272)
                     /locus_tag="U3989_000730"
     CDS             complement(5587..7272)
                     /locus_tag="U3989_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN1714197.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            7547..8572
                     /locus_tag="U3989_000731"
     CDS             7547..8572
                     /locus_tag="U3989_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1714198.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(9167..12841)
                     /gene="rpoC"
                     /locus_tag="U3989_000732"
     CDS             complement(9167..12841)
                     /gene="rpoC"
                     /locus_tag="U3989_000732"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN1714199.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(12899..16516)
                     /gene="rpoB"
                     /locus_tag="U3989_000733"
     CDS             complement(12899..16516)
                     /gene="rpoB"
                     /locus_tag="U3989_000733"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1714200.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            17244..18212
                     /locus_tag="U3989_000734"
     CDS             17244..18212
                     /locus_tag="U3989_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1714201.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            18230..19135
                     /locus_tag="U3989_000735"
     CDS             18230..19135
                     /locus_tag="U3989_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714202.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            19132..19938
                     /locus_tag="U3989_000736"
     CDS             19132..19938
                     /locus_tag="U3989_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1714203.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            20074..21063
                     /locus_tag="U3989_000737"
     CDS             20074..21063
                     /locus_tag="U3989_000737"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN1714204.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(21380..21904)
                     /locus_tag="U3989_000738"
     CDS             complement(21380..21904)
                     /locus_tag="U3989_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN1714205.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            22191..>23255
                     /gene="scm"
                     /locus_tag="U3989_000739"
     CDS             22191..>23255
                     /gene="scm"
                     /locus_tag="U3989_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1714206.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         6708 a         4983 c         4352 g         7212 t
ORIGIN      
        1 tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt
       61 tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt
      121 aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac
      181 gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc
      241 ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga
      301 gggcagccaa tacatgttcg aagtgaaagc gattggctgt acagacactc acgatatcgg
      361 gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc
      421 ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat
      481 ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga
      541 ttgctacttt cttcatttat ttctctctcc tttgcttatt tacttattgt tattctttct
      601 ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg
      661 caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat
      721 ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc
      781 agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat actctttttg
      841 cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat
      901 gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt
      961 gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc
     1021 ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct
     1081 tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac
     1141 gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc
     1201 attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt
     1261 cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa
     1321 caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc
     1381 taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct
     1441 cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa
     1501 gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa
     1561 ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa
     1621 tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct
     1681 tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc
     1741 ccgccatagc cgatccctct taaagaagct tgtaatgcgg cagcactgcc actcgtttca
     1801 ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct
     1861 ttcataggat ctagcgtgta atcggcaccg tatttttctg ctaattctct acgctgcgct
     1921 atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt
     1981 tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc
     2041 cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc
     2101 aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga
     2161 atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt
     2221 tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt
     2281 tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata
     2341 aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt
     2401 actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct
     2461 attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta
     2521 tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat
     2581 aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc
     2641 tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag
     2701 gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga
     2761 aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag
     2821 accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag
     2881 catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt
     2941 atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga
     3001 aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt
     3061 tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga
     3121 gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc
     3181 ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc
     3241 ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa
     3301 tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag
     3361 tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa tagaatcgat
     3421 atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct
     3481 aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata
     3541 gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg
     3601 aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact
     3661 gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac
     3721 aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa
     3781 agctgcaacc tatctactaa cagatcattg acgattccat aactattatt gaacatatat
     3841 ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc
     3901 accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct
     3961 gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat
     4021 tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta
     4081 ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact
     4141 aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa
     4201 tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt
     4261 ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa
     4321 gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct
     4381 gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt
     4441 acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca
     4501 ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga
     4561 gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca
     4621 gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca
     4681 tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct
     4741 tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg
     4801 aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt
     4861 tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca
     4921 tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt
     4981 ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga
     5041 gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt
     5101 tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt
     5161 ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc
     5221 tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca
     5281 aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt
     5341 tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct
     5401 ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag
     5461 tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca
     5521 gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc
     5581 cacctcttat tttattattt tcagccacat atattcatac ggctggaata tcaactttcg
     5641 ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa
     5701 aagataacct gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc
     5761 ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg
     5821 atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc
     5881 tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg
     5941 attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag
     6001 cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc
     6061 taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata
     6121 cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg
     6181 ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc
     6241 tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt
     6301 tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg
     6361 aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc
     6421 gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac
     6481 cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc
     6541 tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg
     6601 aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac
     6661 ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt
     6721 ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac
     6781 gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa
     6841 gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa
     6901 cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt
     6961 gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa
     7021 gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg
     7081 caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc
     7141 ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg
     7201 ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag
     7261 ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat
     7321 tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata
     7381 caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat
     7441 gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac
     7501 tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat
     7561 ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa
     7621 ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa
     7681 ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc
     7741 attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca
     7801 atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac
     7861 accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat
     7921 tattatttta agctctacga tggatgaaac acttcttttt gaattggatc aatacaatat
     7981 tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac
     8041 ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca
     8101 tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta
     8161 ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg
     8221 ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc
     8281 aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca
     8341 atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc
     8401 ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa
     8461 agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat
     8521 tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat
     8581 tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt caatcactca
     8641 tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga
     8701 tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact
     8761 atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt
     8821 atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat
     8881 gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa
     8941 taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt
     9001 tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat
     9061 gttgttaaga tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa
     9121 aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat
     9181 tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt
     9241 cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag
     9301 ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac
     9361 cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga
     9421 aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag
     9481 gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata
     9541 atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt
     9601 gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac
     9661 gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt
     9721 gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg
     9781 ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga
     9841 ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta
     9901 cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag
     9961 gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca
    10021 actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt
    10081 caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg
    10141 tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt
    10201 ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat
    10261 gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt
    10321 catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac
    10381 ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag
    10441 tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca
    10501 agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag
    10561 gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag
    10621 ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt
    10681 gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat
    10741 ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt
    10801 caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg
    10861 catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctt
    10921 ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg
    10981 gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt
    11041 atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt
    11101 caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt
    11161 tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat
    11221 gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca
    11281 ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat
    11341 cttgagaagg agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta
    11401 acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt
    11461 ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc
    11521 gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc
    11581 ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt
    11641 cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa
    11701 gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt
    11761 ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt
    11821 tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt
    11881 tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg
    11941 catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca
    12001 agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca
    12061 agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa
    12121 taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt
    12181 ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt
    12241 cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg
    12301 cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt
    12361 cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat
    12421 atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca
    12481 tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat
    12541 gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa
    12601 cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag
    12661 gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg ttttaaggta cgatagttaa
    12721 tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca
    12781 aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca
    12841 tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt
    12901 agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca
    12961 cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca
    13021 aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg
    13081 tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata
    13141 cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt
    13201 atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat
    13261 aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa
    13321 attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg
    13381 catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca
    13441 cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg
    13501 ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg
    13561 gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt
    13621 gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc
    13681 catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc
    13741 cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac
    13801 gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca
    13861 catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac
    13921 gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc
    13981 cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga
    14041 tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg
    14101 tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga
    14161 tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt
    14221 tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt
    14281 gttccataga tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa
    14341 cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt
    14401 atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa
    14461 cgacaccatc atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca
    14521 taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt
    14581 tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg
    14641 caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt
    14701 ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat
    14761 tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt
    14821 cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa
    14881 atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc
    14941 cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt
    15001 cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggcgaaaaga cgacgtttgt
    15061 gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa
    15121 agaattcttt gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg
    15181 tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga
    15241 tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt
    15301 cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa
    15361 tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt
    15421 ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat
    15481 ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga
    15541 aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct
    15601 cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca
    15661 aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat
    15721 cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac
    15781 gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat
    15841 gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg
    15901 tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac
    15961 ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc caagctccac
    16021 ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga
    16081 aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga
    16141 tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga
    16201 tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg
    16261 catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca taatctacaa
    16321 attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc
    16381 taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta
    16441 attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta
    16501 ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc
    16561 aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct
    16621 tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct
    16681 acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca
    16741 tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc aacacttcct
    16801 ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt
    16861 atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt
    16921 tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac
    16981 ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg
    17041 acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa
    17101 aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc
    17161 aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc
    17221 ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc
    17281 tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac
    17341 aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag
    17401 gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt
    17461 atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag
    17521 aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa
    17581 caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca
    17641 catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa
    17701 aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata
    17761 acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag
    17821 cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga
    17881 aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg
    17941 cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag
    18001 ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca
    18061 aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg
    18121 tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca
    18181 tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt
    18241 gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg
    18301 tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc
    18361 tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg
    18421 tcttctaggc tttttaggga gtgcggcagc agggcttgtt tccggactgc ttcacacaaa
    18481 attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac
    18541 atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg
    18601 ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc
    18661 tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg
    18721 tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa
    18781 gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct tgcttgcgca
    18841 aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc
    18901 tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt
    18961 cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga
    19021 accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca
    19081 gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac
    19141 gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat
    19201 gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga
    19261 aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag
    19321 ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa
    19381 tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa
    19441 gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa
    19501 ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat
    19561 cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg
    19621 atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac
    19681 cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc
    19741 actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc
    19801 atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca
    19861 gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa
    19921 gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc
    19981 ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac
    20041 tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca
    20101 acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag
    20161 ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa
    20221 aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta
    20281 ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa
    20341 acgatgatga cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat
    20401 tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg
    20461 ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa
    20521 cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt
    20581 gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga
    20641 aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt
    20701 catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat
    20761 ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag
    20821 ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg
    20881 gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga
    20941 acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca
    21001 aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa
    21061 taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc
    21121 tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt
    21181 attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca
    21241 agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta
    21301 tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag
    21361 ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct
    21421 gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg
    21481 gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt
    21541 aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag
    21601 cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg
    21661 tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat
    21721 cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga
    21781 tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa
    21841 acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat
    21901 ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct aggataccta
    21961 cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt
    22021 tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga
    22081 acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag
    22141 gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa
    22201 gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg
    22261 tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta
    22321 actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg
    22381 tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg
    22441 gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt
    22501 ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg
    22561 gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg
    22621 atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg
    22681 agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta
    22741 aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt
    22801 tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc
    22861 cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt
    22921 ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag
    22981 atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca
    23041 gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc
    23101 aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc
    23161 ctaacgaaat atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag
    23221 atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DASJON010000025        22172 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJON010000025 DASJON010000000
VERSION     DASJON010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.025"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..331)
                     /locus_tag="U3989_000740"
     CDS             complement(<1..331)
                     /locus_tag="U3989_000740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714207.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(345..602)
                     /locus_tag="U3989_000741"
     CDS             complement(345..602)
                     /locus_tag="U3989_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714208.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(797..1339)
                     /locus_tag="U3989_000742"
     CDS             complement(797..1339)
                     /locus_tag="U3989_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714209.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1360..2451)
                     /locus_tag="U3989_000743"
     CDS             complement(1360..2451)
                     /locus_tag="U3989_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1714210.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2459..4669)
                     /locus_tag="U3989_000744"
     CDS             complement(2459..4669)
                     /locus_tag="U3989_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714211.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4685..7213)
                     /locus_tag="U3989_000745"
     CDS             complement(4685..7213)
                     /locus_tag="U3989_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1714212.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7265..7675)
                     /locus_tag="U3989_000746"
     CDS             complement(7265..7675)
                     /locus_tag="U3989_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714213.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7688..7906)
                     /locus_tag="U3989_000747"
     CDS             complement(7688..7906)
                     /locus_tag="U3989_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714214.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7903..8895)
                     /locus_tag="U3989_000748"
     CDS             complement(7903..8895)
                     /locus_tag="U3989_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1714215.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8974..9885)
                     /locus_tag="U3989_000749"
     CDS             complement(8974..9885)
                     /locus_tag="U3989_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1714216.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9904..10248)
                     /locus_tag="U3989_000750"
     CDS             complement(9904..10248)
                     /locus_tag="U3989_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714217.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10268..10824)
                     /locus_tag="U3989_000751"
                     /pseudo
     CDS             complement(10268..10824)
                     /locus_tag="U3989_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10905..11405)
                     /locus_tag="U3989_000752"
     CDS             complement(10905..11405)
                     /locus_tag="U3989_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714218.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11406..11951)
                     /locus_tag="U3989_000753"
     CDS             complement(11406..11951)
                     /locus_tag="U3989_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714219.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11948..13204)
                     /locus_tag="U3989_000754"
     CDS             complement(11948..13204)
                     /locus_tag="U3989_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1714220.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13565..13876)
                     /locus_tag="U3989_000755"
     CDS             complement(13565..13876)
                     /locus_tag="U3989_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714221.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13899..15209)
                     /locus_tag="U3989_000756"
     CDS             complement(13899..15209)
                     /locus_tag="U3989_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN1714222.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15823..16275)
                     /locus_tag="U3989_000757"
     CDS             complement(15823..16275)
                     /locus_tag="U3989_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714223.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16277..16642)
                     /locus_tag="U3989_000758"
     CDS             complement(16277..16642)
                     /locus_tag="U3989_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714224.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16741..21024)
                     /locus_tag="U3989_000759"
     CDS             complement(16741..21024)
                     /locus_tag="U3989_000759"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEN1714225.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21171..21410)
                     /locus_tag="U3989_000760"
     CDS             complement(21171..21410)
                     /locus_tag="U3989_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714226.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21500..21772)
                     /locus_tag="U3989_000761"
     CDS             complement(21500..21772)
                     /locus_tag="U3989_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714227.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21855..22106)
                     /locus_tag="U3989_000762"
     CDS             complement(21855..22106)
                     /locus_tag="U3989_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714228.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6479 a         4804 c         3308 g         7581 t
ORIGIN      
        1 atgaattcat tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag
       61 acttgtcata caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca
      121 atccattaaa cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa
      181 tttttatacc ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt
      241 gaatttcttg cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg
      301 attcaacgat ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag
      361 ccatgtagag cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt
      421 actctacatt ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca
      481 atgttcggat tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat
      541 acaaacaatc ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc
      601 atctcgtatc catctcctaa aaaatagaat agttcttata gttattcata agacatattt
      661 tttccgtttt tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca
      721 tagtttttct taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga
      781 gtgttcattt tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct
      841 tttgagcttc ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg
      901 tttcttctcc aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa
      961 gtttcccagt tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac
     1021 aacgatctgt catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac
     1081 tccattttct agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt
     1141 gcgctgtctc gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc
     1201 cacttgtttc ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt
     1261 tttcagaaga aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta
     1321 aaattatccc ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag
     1381 actacgaatt tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc
     1441 ggcattctgt tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc
     1501 ggtgtgacca tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc
     1561 tgcttgtaaa tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt
     1621 ataatcttcc cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc
     1681 taattgagat acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc
     1741 aatgatccga tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt
     1801 ttttgttggt aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata
     1861 ggacgttatg atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc
     1921 cgcatagcct ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca
     1981 ttgattaaag ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa
     2041 gttcggaaac ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg
     2101 tgggtaggga caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga
     2161 ttgtgactgt ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa
     2221 ctgatacttt acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg
     2281 tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc
     2341 tagacctgta gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag
     2401 aaaaaaggga agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc
     2461 atcctttctt cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc
     2521 ctggttttgc gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct
     2581 ctgttggctt atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg
     2641 gtttactttc agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg
     2701 ctaccgacga attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc
     2761 catttttcgc ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta
     2821 attgtggcac gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac
     2881 ctttatttaa caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg
     2941 attgtacctg ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg
     3001 aaagaccaga attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat
     3061 gataacgaac atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa
     3121 agggattaac cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact
     3181 tttgaaatcc atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg
     3241 ctaatcctgc acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt
     3301 gtttcatttg agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg
     3361 cagctcctaa ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata
     3421 ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat
     3481 cgtctactat gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt
     3541 gaaacatcaa gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa
     3601 caaaagaagc gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa
     3661 agataaaacg aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg
     3721 gcaccataga accaacgact gctttataaa cagctttagt tggttgaata tagacacgat
     3781 aagccttctt tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag
     3841 ataattcttc acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat
     3901 acaattcttc taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg
     3961 ctttataata aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa
     4021 caccattgac atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc
     4081 cttctacctc cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta
     4141 cgttactacc atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa
     4201 acaccatttt tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat
     4261 acaagacgac aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa
     4321 aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga
     4381 caaaatcaag gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt
     4441 ttagaaaatc tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag
     4501 ataaataaga agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca
     4561 cctgcttttc tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta
     4621 aaaaaaacaa aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc
     4681 atttctattg catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg
     4741 ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac
     4801 gtccataggg atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt
     4861 tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat
     4921 caaaagcaaa gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa
     4981 attgtgtatc taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa
     5041 taatactacg tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg
     5101 ttttcttact actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca
     5161 aacacaaaga ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta
     5221 atttcaaatc ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt
     5281 ctccataaga aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac
     5341 catattctcg aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc
     5401 caacttgttg tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc
     5461 cttttaaaag gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt
     5521 gtttcgcatc tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat
     5581 ttgtagcatc taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat
     5641 gtttctgata atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg
     5701 gatccacaaa taaactttgt acattcataa acgaactata caaaaagagc aagccaacta
     5761 aaaaggtctt ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg
     5821 cacctttgat tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat
     5881 aaattaattc ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt
     5941 gccgaccaat ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt
     6001 cgccaactgc ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc
     6061 ctaataaaca tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa
     6121 tcgtatgaag ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac
     6181 cgtatacaat aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct
     6241 ttaaatgttt tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt
     6301 gaaccaattc cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa
     6361 tacctccctg tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag
     6421 aaagatcctg agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa
     6481 taggcaacaa tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat
     6541 gttccttcgg tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt
     6601 gttcttctac tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct
     6661 ctttttttac taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat
     6721 aaggatcact ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg
     6781 ctaaacttcc tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg
     6841 ttgctttttc tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc
     6901 gttccaacaa tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag
     6961 aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca
     7021 ccaactccac atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta
     7081 tttcctgtgt cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg
     7141 cccagataca atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt
     7201 ttacaagcac caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct
     7261 ttttctaatc ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt
     7321 ttgatacctt gtcccagtta taatacacac aatcaccatt catcatacga atcatataac
     7381 aaaaccaatc ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg
     7441 ctaacacaca taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag
     7501 aaatgaccca accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa
     7561 aagttgctgc attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt
     7621 gtacgcgata aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac
     7681 tatcctccta atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg
     7741 ttggatcatt taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc
     7801 ctacttttga tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat
     7861 agccaccttg cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc
     7921 tttcctatcc aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt
     7981 aatgacaatc gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac
     8041 cgttgctttt acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac
     8101 ttgatcaact ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc
     8161 ttttgcattg tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt
     8221 cgcttccgac gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt
     8281 ttctgtattc tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt
     8341 tgaaatagaa aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact
     8401 ctctggattc atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc
     8461 aatttctttt aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc
     8521 tggttcttga ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg
     8581 ttcttgttca tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga
     8641 ttcaatttca ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg
     8701 ttggatttca tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc
     8761 caagccccct gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt
     8821 tgaatggatt ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag
     8881 ttgtcgtttt ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca
     8941 acgataagct tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt
     9001 tctctttctt ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga
     9061 aagtgcaaca tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt
     9121 tttgatttca atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa
     9181 attcccttca ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc
     9241 acctgctact tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact
     9301 agtgacaatc ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt
     9361 taccgatact gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc
     9421 tgatttttca acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt
     9481 tggctgttcc gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg
     9541 ttccggagtt gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc
     9601 gatcgaatca gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt
     9661 ttcaggtttc tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt
     9721 tagctctgtc tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt
     9781 aggtgtttct acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa
     9841 tgaaattcct agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat
     9901 tttttatgca attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa
     9961 gaactcccgt ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa
    10021 tagttcactg gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc
    10081 atccgaaaat tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa
    10141 aggatctcta taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac
    10201 atagaacaat gccataccac ataccaccaa aaataataat cgccacataa aaattccccc
    10261 ttttcttcta cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat
    10321 ccatcatcaa ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt
    10381 cttcatagcc atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct
    10441 cagcttgcat gacataacca aactctgcac catatcgttc tactgttaat aactgttgac
    10501 tacgcaataa tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac
    10561 gtttgtgaaa taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc
    10621 gataatctgg cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta
    10681 ttcgttcagc ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt
    10741 ttttatcacc tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg
    10801 ctctcctaaa aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag
    10861 aaaccgattt ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc
    10921 aaatgacatt ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg
    10981 gatagaatca cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac
    11041 ttacgcttct tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt
    11101 tctactaagc gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca
    11161 tgtttttcaa tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct
    11221 ggatacctta gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac
    11281 gattctaatc gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct
    11341 ggattttcta atactgttgg atcacgtgac caatacgcac caacataaat cataattttt
    11401 ttcatttaga caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg
    11461 gcatagcgac caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt
    11521 tcttctgttg gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata
    11581 agaggttttt cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag
    11641 ccttttttaa ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat
    11701 tctagccttc ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta
    11761 cgaacttcta ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct
    11821 tttaccgctt gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt
    11881 tcatttaatg cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta
    11941 tatgcattca tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct
    12001 cgtcatttag ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat
    12061 ctgcttcttg taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac
    12121 gatctaatga gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata
    12181 attttgccca tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa
    12241 gttttgcatt aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa
    12301 taaatgcttc tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat
    12361 aaataccata tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa
    12421 aacgtttctc ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata
    12481 atccttcact tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct
    12541 taaagtccat ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat
    12601 gttcttccgt ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca
    12661 cacgtgtata accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc
    12721 gccaatctgt ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa
    12781 attgcacaag tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc
    12841 cacgaacaga aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca
    12901 gatccattcg taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac
    12961 gcaaataatc aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt
    13021 ccttgattcg ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa
    13081 tgtaatcttt tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat
    13141 ctccacttat attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg
    13201 tcattttctc gctcctttta taaaataaca aaatcgattt tttttaaccc tttagaaaca
    13261 ccgatatatc aactagtccc cctcgttttt tgggaaaagt agtacaactg ctgaccttag
    13321 ataatcactt gatacaaaag ggtttataaa acttttttct atgtgttatg ttatttctgc
    13381 cccccatatt atatatcggg gggctttaca caaagaggcc gtgcgcaagc gcacgacctc
    13441 tttgtgagct gcacacgctg ctagcgagtg cgcattatag gcccatgctt cgcagcctta
    13501 atgcgcaccg ccaggcagag tgcggctacc agactacgtc tagagccctc ccgatagata
    13561 ataactacaa aattaaatga tctaattttg ttatttcact atatttttca ggagaaaaaa
    13621 cgcgataaat tgacaaagtt aaatcctctt ctaaatctac tcgataatga tacaatcgag
    13681 tcaatcgttc tatatctttt ttcgttagca atacttgatt aataactttt cctgtaaaaa
    13741 cacgttcttt gcgcaaaaag cggtaatcag ttacttgttc aatacgattc aaccaatcat
    13801 aaaacgttct ggaagaaaga gaaggattaa tcattcgtat tgctgtagct actctataat
    13861 aacaactctc tgccatttaa cttcacctcc ttagttgatt acaaaatatg cgcatcagaa
    13921 aaacttgttc cttttggatc ttttgctaaa tacatttggc gctctagttg ttcttgtaac
    13981 aactgattat catctcccat cgtttgatct tgactatcct cttttactgt aaaatcatag
    14041 acaatcattt cagtaaattt ctcaaattct ttgataaagt gataatcttt gtaaacaaat
    14101 ggcgcaaaaa actctacagg tacatcaaat cctggaaaag catatccacg accaggttga
    14161 tctttgttgt aaaatggctt atttttaaaa tcttctccaa aagtcattaa ataaccaatg
    14221 ggagacaatc gaccaacaga aatacggaat gttaaattat cccgaacacc acttggaaaa
    14281 tattctgcat ctggtcgttg cattgctaaa ataatgtaca tccctgcttg acgacccttt
    14341 aatactaatg gctgaatcaa tttatcgact ctatttctcg cttccatatc caacgaacca
    14401 taaaaagctg cccattcatc aaaaaagata aaatgcggtg gtaacccata ataagcataa
    14461 tttttccctg gttcatattt aggatctttc atggttttca tataggcata ccgttgattc
    14521 atttctttta atgcatttcg taaacaaata atcatttctt catttatttt gtaaaacaca
    14581 tgtcctttga atacatcgac gtcacctaat gctgctaaat ctgccgcttt tccatcgcaa
    14641 atatctactg tcccaacact taaacaagct tgaattaaag aatacaataa ataggttttc
    14701 ccaccaccga ttgcaccagc aatcaacata tttggtgatt gatcatatac ccaatccaaa
    14761 gttttcatta aatgaatacg tccattttct gctatacatt gatcaatagg aatccgacca
    14821 gaatctgaaa ggaatacata attgattcgg gctttttctc gttcttcacg aattaaatca
    14881 cgcaaaaata cttcagataa caaatgaccc atttttaaaa atcgttcgtg aaaacgccca
    14941 ccatctaaag gaaaagaaac cactaattct tgtttctttt gataaaaata aacttttggt
    15001 aatctcattt tttcccttgt ttgtttctca ctaacttgtt tctttgtata aacaaaccga
    15061 ttgtcaatta acattcgaac aagtaactga cgccaataaa cagaggcaaa atatccttct
    15121 tttagtcgcg accaatgcat caaccctaaa attaacccac taaccagcaa caaatacaac
    15181 acaatactta ttccaggaat aagatacatt tgccaattcg aaaacgtact taaacgtgga
    15241 taaaatactt tccaactaaa ccaacctaac agaatcaaaa atggaaacaa aaagaaacca
    15301 taatagcaaa accataaata acgattgaaa tagcgaattc gccgaccacg atacttgaaa
    15361 aaatgagctt tcaattgtca cattcctcct ttctgttttt acttttcctg tgctaaaaac
    15421 ttttgacaaa aagcaactaa tacaggaata tcacatttag taaaaaaaca taatcgaaaa
    15481 agatttaatc gatttttata agggtgtgca cacatattac atattcgtac tatacgaatc
    15541 caaatcactt cctgatgatc ttgtttccac tggtacggta atgaaaaacg aacaagatca
    15601 cgtaaaaaca tacactgccc aataaccgca caaatatatg catttgtttg tacaaaaatc
    15661 gttggattgt ctccaaaata ctcaatcaat tgttggatat tttgataaca agcacaatct
    15721 ctcaaagtat ttgattggtt ttatctgaat tcaaaatctc cccttccttc tatcttagat
    15781 aaaaagaata ggaagactcc ttcctattct ttcaaaaaaa ccttattttt tctctttttc
    15841 cttagaatca acacttggtt gtttttgatc taaacctttt tcttgtgatt gtttcatatt
    15901 aggattattt gttggagctt ctactttctc taatcctttc gcataaatca ctaaatctga
    15961 atattcacga ttgccatctt gtgctggaat atataccaca tgatattctg gatcaattaa
    16021 tttcacagga tcacgtcgtt taaaaccagt caaatctaca tctgctggaa atttaactcg
    16081 tcgtgcaatc tttggttcat ctggatttcc aaaaactaat tgtgctcgct gtgatcgaat
    16141 ttctcccgta taatcgcctg tttcattatc aaatacacga tcagactctt ctaatgcgcc
    16201 aaagcgtacg gctcctaaac aagtttcatc taatgcttct gtaatgtcat aattatttgg
    16261 aaattttaat cccatcttac aaaacctccc caatttttac accagttaaa aagacaccaa
    16321 catcttgata gccagttgtt cgacgctctg tttgtcccat atcatttgtt tcacgactat
    16381 tcactcgttc tttacgaaaa acaaaataag cttcttcaaa tgaaatacgc acctcttttt
    16441 ctggtaatcg taatctttta tcctcttcag ctgtgagata aacaaaaaaa gcaggctgtt
    16501 cttctcgcga acactctgct tgtaattgaa catatcgccc agacacacca ttaatttcct
    16561 ttggtggatt aggttcccgt gaacccaata aaaatacttg acctaaaaac tctttcttca
    16621 tacgcaatgc tttttctgcc ataaagtttt tcctcctctt aaaaaccaaa ataaattcta
    16681 aatatttcta cattacaatc aaacaagcgg ttaaaaatcc taaccgcttg ttcctcatga
    16741 ctaatctttg gctttacgta ctttataaag tgttatacct aaagcaatga ctaatactac
    16801 taatccaaga accataagta tactacttgt ttgttcacct gtttttggca acattggtac
    16861 aacagtagct ggtttaaccc gttgttctgg tttcactggt tgcggtggtt ttggcgcttc
    16921 cacaatttga accgtttgat ctttgtttgt tagatctaca ttgtgtttcg ccacttcttc
    16981 aaacgtttct ttctcatctt ccgttttcgc ttggagccat tccgcaaaac tcaagccttt
    17041 tcctgcaaga ttgcgaccgt ccactaagaa ttcaaataag aattccatca tttctttatc
    17101 ggccgcaaag actttttccg catgagtgcc ttcaaatggt gtgccgtctg gtgttaagta
    17161 gccttgaaca attaagcgat agatttgacc aatcgcaaga ccgtctactt tgacccattc
    17221 aagcacagtt tgatttggca aggccaagat ctcttttccg ccatttttat tcgtcgcttt
    17281 cgtcgtaatg gttggattac tgattttgat ggtttggtca ttgtcggtaa tatctgcgtg
    17341 agaagccaat aatacgcctt ctctaaacag atcttcaaag accacgactt ctttgcctcg
    17401 caacgcacgt gcgtctagaa taaagtctag attgactgta ccatccgttg tttctgccac
    17461 aaacgttaag gtacttgtga cttcttttcc atctactagc actggttttt ccgttacttt
    17521 gtccattaga acaccgttga ctgtgtatgt ttttccaacg accaaatcgt ggtatttcac
    17581 agcgtctgta atggtgactt tcgttaatgg attaacctct ttcaagccag tgggtttatg
    17641 tgtagccgtt gtacgtacac ttgggttagt tactcgtacc gtttgccctt tgtcgttgat
    17701 gtctttatgg tttgctagtt cttcatcatt tctaaaaaga ctttcaaaca cgacgatttc
    17761 acgaccattt atacctgtca aatcaaaggt aaatggtacg atcaccgttt cattttctgc
    17821 tttggcaata aagccaacac tcgtttctac cgtatttcca tgcacaagca agggttcacc
    17881 ggttgcttta tccattaaga tccctttgac caagtatgct tttcctggaa gaatctcggt
    17941 aatttcaacg gtatcgttta aggtaaacaa tcctgttggg tctgcgactg tcaatccgtc
    18001 ttcaaagaca gccgtggtat gaacggttgg attattcact ttcacacttt gggcttcatc
    18061 gttgatatcc gcatgtgctg caacttcaat gccatcacgg aaaagttttt caaaaacaac
    18121 gatcgtttga cctttcagaa cactagcatt aaccacaaat tcaaggtctt ttgtgccatt
    18181 gactttgtct gcaataaagg ccaaggtact tgtgatttct ttgccatctt gtaaaattgg
    18241 cgcattcgtt gccttgttca tcaatactcc tgaaatggtg tattcttttc cgacaaacaa
    18301 gttggtataa gaaacactgt ctttaatgac gatcgtttct aatggatcaa ccactttttc
    18361 accatcggct ttatttgacg cttgtgtttc tagttttggt ggaatcaagt aattacgtac
    18421 agaaaccact aacgtttccc catcatctgg cgcattttgt ttgaatgcaa ccgtgaatgc
    18481 ttgtttatct gtatttggtt ggaaactttc taatggggca ttttccaaga agtaatagtc
    18541 atcggcaact agacctttca ctaaaatttc cccgttttga tcagtggtat attcaccaat
    18601 tgcttgattt ttaccgttat acaaggtaaa gttcacacct gcgagcggcg ttttcttctt
    18661 gttccattct tcatacttga ataattttac acttttccgt gcttgacggt tcgcaaaatg
    18721 gatcactttg atcggttctg tatcattggt aatcgagaac actaatgggt tttgattcaa
    18781 aacgtaattt acgggggctt tgatttcttc caaacgatag cgatcttttc cataagtaag
    18841 tgtaccgtta gttaggaaaa agcctttttc attggtttta aagatatccg ttgtttctgt
    18901 atcatttgga atctccattg tgacataacg attggctaaa gtatcaaaaa tcttgaattc
    18961 tgtattagca agtggaatca cttttcctgt ttcttgatcc actttcacca ctttaagacg
    19021 ttgttcaatt actttatctt ctaaaacata atggaaagtt tgcccatttt cagaaattgt
    19081 gacttcaagt ggttcaaata gtttataccc ttcttttcct ttggtttcag aaagaatgta
    19141 cgtatcaaag acaacggtat ttttaccaaa agtagcataa ccatcattgt ctgtcacaac
    19201 ggttttcacc actttgccag ttgtttttga cgtcaaagtg atttcaatgc cagctaattt
    19261 tggtttgtta tctggattcg ttgaatcgac taaatcttta ttagcaactt tcactaaatc
    19321 aatggttcct ttgatcactt gatcttttgc ggtcgcattg acagtttttc caggttcaac
    19381 aacaatttca attgcttcat cagaaagcac ataacctttt ggcgctttga tttcttttaa
    19441 aacatatttg ccaggatcaa gatctttaat ttctgcctgt actttgccgt taattttttt
    19501 caaggtaatt tctttaatct ttttcccatc tgcttgatac aaaccataga ccgcattttc
    19561 taaagaagct gcaccttgtg ctttcacacc agtttccgca tcttctttaa acacacgaat
    19621 gtttccttgt tttagaattt gtaaattaat aacagacgca ttaggttctg ttggatgtaa
    19681 cacaccaatt gtttgagctc ctggacgacg ataggctaat ggcgcacctt gaactttaat
    19741 tttattcaat tgaatttttg ctttatccgg tgcctctttt gtgactgtaa tccatacttc
    19801 attgccgtga atagatacat tgacaccttt tggtttttga atgtctttca catatttcaa
    19861 aaaatcattt ttattcttta ctttcaacgt ttcgccaatt tttaccgtat gtgtttgatt
    19921 attaaaagtt gctttttcac caaagttatt aatcaaacgt tgaatttctg cttttcgacg
    19981 atcataatca acacggatat ttttgattgg gatttttgtg cccaaagctt cccaaatcat
    20041 aaattgtgcc gcaatgtatt gttcatcgga tttgtcttta tttgtgacat atcctaaata
    20101 agagatcaag cttgtttttt tccgtacatt tgaatttttc aaatagtttt tcaaagcagt
    20161 agctgtataa acttgacctt ctgcggctga gacaccaggc tcaatacaaa acgccacttg
    20221 tccattaatc tcaaaacgaa ccatataatt taaagtgaag tttagattgt tcggactaga
    20281 agtaacacca atattttcgc cccaatcaat tttccgacca cctaaaacag ctgcattggt
    20341 tgcacggcga acacgaaccg aacgattggt tggtactgtt actaacagtg taggtgtacc
    20401 tgtttttgga atgttctgct cttttttctt ttgattttca atactagccg tagattcttt
    20461 gtttggtttc gttggttgat tttgactagc tgcggttgaa gagattgttt tttcaacgtt
    20521 ttcctcattt tttccttctt tgtcagcagt agccgctggt tgttccactg tttcttctat
    20581 tcgtttcttt tcttccgctt gtttctcaga agactctggc tgcacagaag gcgttgcttc
    20641 ggcagctttt tctccttcta gagtggacgt ttctgaagca ggggttgtaa ttgaggcagt
    20701 ttcttcaact ggttgtgaag taccaggact attccctggg tgttgtgttt ccccaatcgt
    20761 ctgatcgaga gtcgttttgg aattgaccgt tggcgcttct gtcacagggg cctgttcaac
    20821 agtggcttca ccagctggga ccccggcttc tgctggttgg acttctgtca cttcagaagc
    20881 gacttgggca atggcttcca tcgtaggttg tatagccgtc gtttcttctg catgtgcttg
    20941 agcagttccc accaagaaac agaattgaga aaaagccacc aatgcgagtg ctttcaacca
    21001 attcattttc gtttttgcat acacaagaat tcctcctttt cctgagaatt ttatataata
    21061 aaacaaaacg ccgttaccaa aagtgataac gacgtgaact gtctcttcta tttttatctg
    21121 gcgctcttat ctgaacacat ttcatcaatg attttaccct cccactataa ttacagaaca
    21181 aaaaaagaac ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagctttt
    21241 ttggcaactt caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt
    21301 tctttccaac catatgacat agcttcatct aatgaatcaa aaatcatgac actattgcca
    21361 attgttacag gatctggctt aagatcttct ggaagagaaa tatcaatcat accataatga
    21421 tccgattcct cagtattatt gtcattacta gctactggtt tggttgttct actggcttat
    21481 tttgatacaa caagtactct tattcttctt ttacttctcc gttttcaatt tcccattcaa
    21541 accactccgc aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat
    21601 cttctgacgt cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta
    21661 cccatgtttc gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa
    21721 tctcccgaac ttgattttta attattgata cttgttcaaa accatcactc attctatcac
    21781 ctcaaataac tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc
    21841 taacaaatcg ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat
    21901 tttccataaa cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg
    21961 ttcttatctt ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa
    22021 tcacaccacc catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt
    22081 gtattaaaca ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta
    22141 ggaatcacaa cttattcgct tcttctttat aa
//
LOCUS       DASJON010000026        21889 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJON010000026 DASJON010000000
VERSION     DASJON010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.026"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..716
                     /locus_tag="U3989_000763"
     CDS             21..716
                     /locus_tag="U3989_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN1714229.1"
                     /translation="MVMPVTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSET
                     GLQASQQTIAVTPEILVGVSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYS
                     PSFQPDSVRTPEDFQRFLEREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLW
                     MTRKSQLSSIHTFKESANLMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLW
                     VFVKTRFKDKRVE"
     gene            867..2033
                     /locus_tag="U3989_000764"
     CDS             867..2033
                     /locus_tag="U3989_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1714230.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            2263..2910
                     /locus_tag="U3989_000765"
     CDS             2263..2910
                     /locus_tag="U3989_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1714231.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2994..3938
                     /locus_tag="U3989_000766"
     CDS             2994..3938
                     /locus_tag="U3989_000766"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN1714232.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            4073..5524
                     /locus_tag="U3989_000767"
     CDS             4073..5524
                     /locus_tag="U3989_000767"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN1714233.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            5545..6381
                     /locus_tag="U3989_000768"
     CDS             5545..6381
                     /locus_tag="U3989_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1714234.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            6381..7040
                     /locus_tag="U3989_000769"
     CDS             6381..7040
                     /locus_tag="U3989_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1714235.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            7166..7855
                     /locus_tag="U3989_000770"
     CDS             7166..7855
                     /locus_tag="U3989_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714236.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            7861..8817
                     /locus_tag="U3989_000771"
     CDS             7861..8817
                     /locus_tag="U3989_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN1714237.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            8861..9373
                     /locus_tag="U3989_000772"
     CDS             8861..9373
                     /locus_tag="U3989_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1714238.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(9629..10177)
                     /locus_tag="U3989_000773"
     CDS             complement(9629..10177)
                     /locus_tag="U3989_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1714239.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(10266..11447)
                     /locus_tag="U3989_000774"
     CDS             complement(10266..11447)
                     /locus_tag="U3989_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN1714240.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(11683..13191)
                     /locus_tag="U3989_000775"
     CDS             complement(11683..13191)
                     /locus_tag="U3989_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN1714241.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            13350..13541
                     /locus_tag="U3989_000776"
     CDS             13350..13541
                     /locus_tag="U3989_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714242.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            13744..14661
                     /locus_tag="U3989_000777"
     CDS             13744..14661
                     /locus_tag="U3989_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN1714243.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            14654..15727
                     /locus_tag="U3989_000778"
     CDS             14654..15727
                     /locus_tag="U3989_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714244.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            15875..16393
                     /locus_tag="U3989_000779"
     CDS             15875..16393
                     /locus_tag="U3989_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714245.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            16374..16766
                     /locus_tag="U3989_000780"
     CDS             16374..16766
                     /locus_tag="U3989_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN1714246.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            16842..17777
                     /locus_tag="U3989_000781"
     CDS             16842..17777
                     /locus_tag="U3989_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN1714247.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            17910..18689
                     /locus_tag="U3989_000782"
     CDS             17910..18689
                     /locus_tag="U3989_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1714248.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            18682..18999
                     /locus_tag="U3989_000783"
     CDS             18682..18999
                     /locus_tag="U3989_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1714249.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            19243..20853
                     /locus_tag="U3989_000784"
     CDS             19243..20853
                     /locus_tag="U3989_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1714250.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            21010..21858
                     /locus_tag="U3989_000785"
     CDS             21010..21858
                     /locus_tag="U3989_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1714251.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         7102 a         3888 c         4367 g         6532 t
ORIGIN      
        1 ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc
       61 aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc
      121 aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag
      181 ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg
      241 gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta
      301 tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg
      361 aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt
      421 tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg
      481 gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca
      541 aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg
      601 tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct
      661 tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag
      721 tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa
      781 aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata
      841 gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt
      901 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
      961 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
     1021 agattacaag taacgcttct ttggcactag gagaagccta tatggatcat cgaatcgaga
     1081 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
     1141 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
     1201 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
     1261 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
     1321 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
     1381 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
     1441 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
     1501 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
     1561 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
     1621 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
     1681 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
     1741 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
     1801 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
     1861 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
     1921 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
     1981 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
     2041 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
     2101 acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
     2161 cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt cttcgttata
     2221 cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa aactgttatt
     2281 tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac caatcaaggc
     2341 acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc ggactcctca
     2401 acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc aagtactcag
     2461 tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa atacccagat
     2521 actgcaatta ctagtttgga attagatagt gattggggat cttacttcta caagatagaa
     2581 ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga aaaaatggag
     2641 acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat
     2701 ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa
     2761 gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac tacttattgg
     2821 gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag ccaaacaggc
     2881 gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag agttgatttt
     2941 tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt tacttgagtt
     3001 tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca tctggtgtcc
     3061 actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga gcatttatca
     3121 ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat tttgatgttc
     3181 ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat tatctagagt
     3241 atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt ttttccattg
     3301 ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa agtgggttta
     3361 atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca caactagctt
     3421 acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt tctaagccgc
     3481 taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg gctgatatct
     3541 tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata
     3601 tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc ggcattggcg
     3661 gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca cgtttgaaac
     3721 cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca tttgaacatc
     3781 tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa gggccggaaa
     3841 ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga tatacgtcaa
     3901 ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa ggtcatgatt
     3961 gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat
     4021 tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga
     4081 tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac
     4141 ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga
     4201 accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc
     4261 tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc
     4321 aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc
     4381 agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa
     4441 tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc
     4501 gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg
     4561 tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct
     4621 gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc
     4681 tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat
     4741 gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc
     4801 aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag
     4861 tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga
     4921 tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat
     4981 gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt
     5041 cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc
     5101 tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa
     5161 cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat
     5221 cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc
     5281 tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc
     5341 ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac
     5401 aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct
     5461 atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa
     5521 atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt
     5581 gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg
     5641 agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca
     5701 tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg
     5761 gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact
     5821 tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga
     5881 gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga
     5941 aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc
     6001 gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat
     6061 gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt
     6121 ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa
     6181 atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat
     6241 gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca
     6301 aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg
     6361 gctgagtcgg tgagtgagtg atgtactttc ccctaattga tttttccctc atgtggactt
     6421 cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc
     6481 ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
     6541 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
     6601 tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
     6661 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
     6721 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
     6781 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
     6841 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
     6901 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
     6961 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
     7021 ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
     7081 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
     7141 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
     7201 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
     7261 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
     7321 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
     7381 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
     7441 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
     7501 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
     7561 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
     7621 aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat tgaaaagacg
     7681 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
     7741 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
     7801 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
     7861 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
     7921 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
     7981 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa
     8041 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
     8101 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
     8161 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
     8221 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
     8281 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
     8341 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
     8401 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
     8461 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
     8521 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
     8581 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
     8641 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
     8701 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
     8761 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
     8821 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
     8881 gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc
     8941 gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga
     9001 tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc
     9061 attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac
     9121 tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact
     9181 agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat
     9241 ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt
     9301 cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat
     9361 aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa
     9421 aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa
     9481 agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc
     9541 tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac gatctctcgg
     9601 cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt attttatctg
     9661 cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac
     9721 gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg
     9781 aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta ataaagatag
     9841 gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct gtcaaaacgg
     9901 cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa ccatatttat
     9961 gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga ttgaatgtat
    10021 tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt gatcgattcg
    10081 tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa gtttttccag
    10141 aaccagaagg accgataaat acatagcaat aattcattgt catttctcct ttgatgattg
    10201 aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact ttgctttttt
    10261 gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa agtcgctgtt
    10321 agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt taatcccttc
    10381 atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag gaagacagat
    10441 ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc tgtcatagca
    10501 acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac tgcttctttc
    10561 catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa
    10621 gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc aacaaaaccg
    10681 ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta ttctcccaac
    10741 atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc tttttcttct
    10801 aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt caaaacaagg
    10861 gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat cgacatagaa
    10921 actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag gaagggtaat
    10981 tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc tactccatta
    11041 taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac agctttcata
    11101 aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat aaatacaatt
    11161 ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca
    11221 aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc caatacagaa
    11281 acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg
    11341 aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat caaaaaagca
    11401 agccctaatg tgacaaataa accaataata cctaataaat aactcaaata atcactccta
    11461 ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt tagtgaaatt
    11521 ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa gcgaacaaaa
    11581 taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa actatcgatt
    11641 aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt aatatagatt
    11701 ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca atgtttgttc
    11761 atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt cagctagttt
    11821 atcataagca acttgagcga ttggattcgt tactgcactg attgataaag cgatcaagac
    11881 ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca atccttgtat
    11941 tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg ctaaatgttt
    12001 tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac gcccggtaat
    12061 gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg taggactttg
    12121 cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt cttcaggacg
    12181 taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt cgatcaatcc
    12241 ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt tagaggcttc
    12301 ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca tgtcagtagg
    12361 agattgataa acagattctt taccagtaat tttttcaata atacgtttga ttacaggaaa
    12421 tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa agtggaagga
    12481 atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa tgttcagcgg
    12541 atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac cagctgcatg
    12601 tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc cacttcctgg
    12661 gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat tttttccaaa
    12721 accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt ctgagtgttt
    12781 atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg caggctgtcc
    12841 actatagcga gtgataacga cactgttgat taataagcca taaccacgta attcatcgat
    12901 taaccgcaag atatccatat cataagtaat cccataatca ccacggatct tattacgttc
    12961 aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt tttgcagcaa
    13021 tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac
    13081 taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt ccaaaatata
    13141 cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca ctaggttccc
    13201 tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa
    13261 ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt attcattggt
    13321 atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat
    13381 ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa gatcccgaat
    13441 tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt gatgagcatc
    13501 agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga taaaggtcat
    13561 cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa ggctgtacca
    13621 aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac aattttttac
    13681 agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga ggaggcaagt
    13741 agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga ggagacgaac
    13801 gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga aaaagcaagc
    13861 atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt ccgcgaagaa
    13921 gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt aaaaagaagc
    13981 tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga tcgtttttta
    14041 caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag ctattacaaa
    14101 atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct gccagaaatt
    14161 tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga gtcaacggac
    14221 tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac agaaattgaa
    14281 cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa cgacaataaa
    14341 ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa gaagaagcaa
    14401 gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga aacaatccag
    14461 cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc agccatcgta
    14521 gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt cggtcaagca
    14581 atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca atctaatgaa
    14641 ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg aagaacaaac
    14701 agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc ctctggatga
    14761 cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa aagtaactcc
    14821 gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa atcccgggta
    14881 tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc
    14941 agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc cacaattatt
    15001 agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg gaaagacaag
    15061 cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg gtctggaacg
    15121 gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt atacgttgaa
    15181 tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac gataccaagt
    15241 ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg tccaagatga
    15301 ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg cagggcagat
    15361 cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac ttggttttac
    15421 cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc agagcgaatt
    15481 tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa atccatccta
    15541 cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag gacagtcttt
    15601 aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg gagactaccc
    15661 acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa tggtgagaaa
    15721 gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt ccaaaggact
    15781 tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa atggacgtga
    15841 ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa tagcacagct
    15901 tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta ttccgaattt
    15961 ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa aagatgcgtt
    16021 aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg gaccatgggt
    16081 catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg tctgcttgag
    16141 aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg cgggcatggt
    16201 ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc aaccttggat
    16261 ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg ttgtgttctt
    16321 tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa
    16381 aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca agttgcagta
    16441 caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa
    16501 caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc ttcaaaaaag
    16561 gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat aacggcttca
    16621 tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg atgtttactg
    16681 tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat ggacaatcgg
    16741 tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg cgaatattgc
    16801 cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa
    16861 gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct atcagcaaat
    16921 ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg
    16981 ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat aaaggacaga
    17041 gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca atcgtcgcaa
    17101 tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa aagttgacgc
    17161 atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta tttggtttgg
    17221 taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa
    17281 aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg attattttta
    17341 cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca atattgactt
    17401 atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt ttcttgctga
    17461 tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt gaaagcgtga
    17521 cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc agtcggctga
    17581 tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca aaagcagata
    17641 tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa aaaaatatgc
    17701 gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa tatgacccaa
    17761 attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga aaaatataat
    17821 cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga gtccgctgtt
    17881 tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc
    17941 aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga ttagcttgcg
    18001 gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta tcgattctgg
    18061 tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa gcatcatttg
    18121 caacgacatt attgatggtt ttctttttag aattacgcta tacattatta ggagcaagtc
    18181 ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct caatcactga
    18241 atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc tggaataaaa
    18301 gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc aatatgatcg
    18361 gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt gctttgacgg
    18421 ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc tttacaggtt
    18481 tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact ttcggattga
    18541 ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca ttcaaaaaag
    18601 aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc
    18661 cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt tgcttgtctg
    18721 ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca tcgaaaagtg
    18781 ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc ggcgttagct
    18841 tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat caaaatagct
    18901 gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg
    18961 acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt ttattaaaaa
    19021 cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg caacagtcaa
    19081 ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta tgatgcaagc
    19141 tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt gctataatag
    19201 ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa aaaattgatg
    19261 caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg ggtagtctat
    19321 ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc tacgacattg
    19381 tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt cggatttcca
    19441 aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc tgcttgtcgg
    19501 ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc tgctgcactg
    19561 atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt taataatggg
    19621 atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct tagtgcaatg
    19681 agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt gattatcgaa
    19741 caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt attgactgac
    19801 ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg tgggttagct
    19861 gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact taaagatttt
    19921 ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc tatcattcgg
    19981 gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat gatcgaccaa
    20041 gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca gcaactggaa
    20101 ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct gatctccatg
    20161 gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa aaaagatcgg
    20221 atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat tcttcttcct
    20281 tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt tggctatgat
    20341 cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt ctctttattc
    20401 acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat ccgattaact
    20461 gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt agttggcgga
    20521 tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg cggctatcac
    20581 tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt tttcttaaaa
    20641 actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt tttgctgaat
    20701 cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc tgtaggaagt
    20761 attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa
    20821 aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt tgctgtcact
    20881 tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa ttttagtttc
    20941 atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg aaacgaggaa
    21001 attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc ggaacgtatc
    21061 aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg gaaaatttat
    21121 aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa cagtaaagat
    21181 tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa
    21241 gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc tggctttgtc
    21301 aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc ttatgactct
    21361 aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt aaatgatgaa
    21421 ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa tacctatgct
    21481 tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac aaatggtgca
    21541 gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga agggaaaatt
    21601 gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga agaaattatt
    21661 ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca tgcaaataat
    21721 ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc
    21781 gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc catcggcatt
    21841 gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaata
//
LOCUS       DASJON010000027        21833 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJON010000027 DASJON010000000
VERSION     DASJON010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.027"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="U3989_000786"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="U3989_000786"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN1714252.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="U3989_000787"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="U3989_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN1714253.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="U3989_000788"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="U3989_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN1714254.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="U3989_000789"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="U3989_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN1714255.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="U3989_000790"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="U3989_000790"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN1714256.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="U3989_000791"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="U3989_000791"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN1714257.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="U3989_000792"
     CDS             complement(8054..8524)
                     /locus_tag="U3989_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN1714258.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="U3989_000793"
     CDS             complement(8570..8995)
                     /locus_tag="U3989_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN1714259.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="U3989_000794"
     CDS             complement(9062..9331)
                     /locus_tag="U3989_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN1714260.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="U3989_000795"
     CDS             complement(9342..10937)
                     /locus_tag="U3989_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1714261.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="U3989_000796"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="U3989_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN1714262.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="U3989_000797"
     CDS             complement(14490..15050)
                     /locus_tag="U3989_000797"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN1714263.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="U3989_000798"
     CDS             15403..16377
                     /locus_tag="U3989_000798"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1714264.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="U3989_000799"
     CDS             16547..17947
                     /locus_tag="U3989_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN1714265.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="U3989_000800"
     CDS             complement(18025..18747)
                     /locus_tag="U3989_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1714266.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="U3989_000801"
     CDS             complement(18826..19332)
                     /locus_tag="U3989_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1714267.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="U3989_000802"
     CDS             19455..21254
                     /locus_tag="U3989_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN1714268.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..>21833)
                     /locus_tag="U3989_000803"
     CDS             complement(21322..>21833)
                     /locus_tag="U3989_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1714269.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6049 a         4791 c         3964 g         7029 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttg
//
LOCUS       DASJON010000028        21484 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJON010000028 DASJON010000000
VERSION     DASJON010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.028"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(524..667)
                     /locus_tag="U3989_000804"
     CDS             complement(524..667)
                     /locus_tag="U3989_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN1714270.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(890..1369)
                     /locus_tag="U3989_000805"
     CDS             complement(890..1369)
                     /locus_tag="U3989_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1714271.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(1442..1726)
                     /locus_tag="U3989_000806"
     CDS             complement(1442..1726)
                     /locus_tag="U3989_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714272.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(1804..1998)
                     /gene="ssrS"
                     /locus_tag="U3989_000807"
     ncRNA           complement(1804..1998)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U3989_000807"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2070..3356)
                     /locus_tag="U3989_000808"
     CDS             complement(2070..3356)
                     /locus_tag="U3989_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN1714273.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            3494..4165
                     /locus_tag="U3989_000809"
     CDS             3494..4165
                     /locus_tag="U3989_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN1714274.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            4131..4619
                     /locus_tag="U3989_000810"
     CDS             4131..4619
                     /locus_tag="U3989_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN1714275.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            4632..5579
                     /gene="prmA"
                     /locus_tag="U3989_000811"
     CDS             4632..5579
                     /gene="prmA"
                     /locus_tag="U3989_000811"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN1714276.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            5580..6344
                     /locus_tag="U3989_000812"
     CDS             5580..6344
                     /locus_tag="U3989_000812"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN1714277.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            6577..8790
                     /locus_tag="U3989_000813"
     CDS             6577..8790
                     /locus_tag="U3989_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN1714278.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            8801..9247
                     /locus_tag="U3989_000814"
     CDS             8801..9247
                     /locus_tag="U3989_000814"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN1714279.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            9418..10677
                     /locus_tag="U3989_000815"
     CDS             9418..10677
                     /locus_tag="U3989_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN1714280.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(10766..11713)
                     /locus_tag="U3989_000816"
     CDS             complement(10766..11713)
                     /locus_tag="U3989_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1714281.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            12107..13399
                     /locus_tag="U3989_000817"
     CDS             12107..13399
                     /locus_tag="U3989_000817"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN1714282.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            13414..15183
                     /gene="aspS"
                     /locus_tag="U3989_000818"
     CDS             13414..15183
                     /gene="aspS"
                     /locus_tag="U3989_000818"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN1714283.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            15292..16167
                     /locus_tag="U3989_000819"
     CDS             15292..16167
                     /locus_tag="U3989_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1714284.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            16433..18316
                     /locus_tag="U3989_000820"
     CDS             16433..18316
                     /locus_tag="U3989_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1714285.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(18391..18675)
                     /locus_tag="U3989_000821"
     CDS             complement(18391..18675)
                     /locus_tag="U3989_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1714286.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(18665..19399)
                     /locus_tag="U3989_000822"
     CDS             complement(18665..19399)
                     /locus_tag="U3989_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN1714287.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            19528..20151
                     /locus_tag="U3989_000823"
     CDS             19528..20151
                     /locus_tag="U3989_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN1714288.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            20343..21464
                     /locus_tag="U3989_000824"
     CDS             20343..21464
                     /locus_tag="U3989_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN1714289.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
BASE COUNT         6889 a         3878 c         4598 g         6119 t
ORIGIN      
        1 atttaaactc agattatcat gagaaaaata ccttgagatg gccattgatt ttacgtgaat
       61 tcaactgttt tggacaaact tgtcccgtca acagcaactg taaaaccaat tgcatggcta
      121 aatgcgatat ggtatcgttg gtaattttca ttacttgtta ttttagtcat gcgcatcata
      181 gtattttccc tccttcgtgt agttgtcttt tccagtgtac ctgaaagtgt cagaaagttc
      241 aactagcgct gacatttact taatatttat cgatgtgtaa ttctgtcaga gagctttttt
      301 tatctgactg agggtaatgt ctcgtggacc acatcattgt attctgagct aaaccagcgt
      361 tcaggcgaaa tcatatggtg tttgataaaa aactttgtct acatgacttc cttttgccat
      421 ttgcttaata ccgttgcatt aaacagctct tctctttgga aatctcacca acatcgcgtt
      481 caggagtatc gttgtggttg caatggcaag cggtagcctt tcatcaattc ttgaatgatt
      541 taccgaatgt agcacttgcc ttctcgcggt acccgaaccc gaaataaaat tttagtttgg
      601 cgataaaatc ggtatccgcc atcgatactt catatttttt cgtatcagat tcatcggaaa
      661 cttccacatt gacgaaccaa taagctttgg catgcttagg tcgttttaaa ccacaccagg
      721 gcgactaatc gccctggtgt tcactttttt ttattttact tgaactcatt gactgttttc
      781 cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg tttttatcac
      841 ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt taagctttgg
      901 tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc acatcgcaag
      961 ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct acagcattca
     1021 atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt tctctggcaa
     1081 taatttgttt tggtgagcca tattctacga cagaggttac attattcaag ccagctttct
     1141 tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct tgttctgcaa
     1201 gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca tgtgctaata
     1261 gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct aattcagctt
     1321 catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata agcattcact
     1381 cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa aagagaatta
     1441 tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg gcagaaacaa
     1501 taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa gctgttaaaa
     1561 tacccattac gcctaaaaca gcatatgcag ctccgtaaaa ttgtaggaat ccttgatttt
     1621 tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa taatagctaa
     1681 cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac aaatcctcca
     1741 aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat tctttaaaag
     1801 atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg aattcaagca
     1861 ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta ccagcagtga
     1921 cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa gaggacaatt
     1981 cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga gcaatgagag
     2041 agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt tttccactct
     2101 tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc ttttggctga
     2161 taatactgag cattcttcag tttatctggt aaatattgct gattgaccca agcctgatca
     2221 aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc tttgtaatgg
     2281 ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc agcaagtgct
     2341 gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc gacgacgacg
     2401 tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa tacagcgttc
     2461 actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc gataaccatc
     2521 agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag ataatgaagt
     2581 gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac gtcataatga
     2641 gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc aatgatcgac
     2701 agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc cagttccagc
     2761 ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag aagagcatct
     2821 tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg
     2881 actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg tgtacggctg
     2941 cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat caagatgatt
     3001 cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa tcggtgtact
     3061 tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac aacttgtaga
     3121 tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt agagccggcg
     3181 atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat catggaagag
     3241 agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag atgctgctga
     3301 ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg ttgcatagga
     3361 ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag atgcctagaa
     3421 agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt gtatgataaa
     3481 gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga ataaaacgac
     3541 gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac cagaaggaag
     3601 attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg aggccgccca
     3661 tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat
     3721 ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc cagaagggat
     3781 tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc aaatgagtaa
     3841 aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat tagtggaagc
     3901 attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt tacgcttagt
     3961 ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg gtatcccaaa
     4021 caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc cttatatctc
     4081 tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga
     4141 aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac tatgatgtag
     4201 ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa
     4261 ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg
     4321 aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt
     4381 atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta gctgattctt
     4441 tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt gattttttgg
     4501 atgaagacga tgacgaagct gtattcgagc ttcactggga taatcagcaa ctagcagaat
     4561 tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca agttattaag
     4621 gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag
     4681 cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa gattctttag
     4741 atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat gattttacat
     4801 cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc ttaccggaaa
     4861 tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta gctattggaa
     4921 aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc tggaagaaat
     4981 actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg gaaaactacc
     5041 agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca tttggcacag
     5101 gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg cgtggtggtg
     5161 aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca aaatatcttg
     5221 gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa
     5281 atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat cttttgacag
     5341 gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc atcttattga
     5401 tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt tcaggaatca
     5461 ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt atcgtggatc
     5521 aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag gtggaataga
     5581 tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt ttagaagggg
     5641 aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa gtttttttgg
     5701 cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa gaggctgttt
     5761 atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg attaccatag
     5821 cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc actgaattag
     5881 gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa
     5941 aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg gaacaatccc
     6001 atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta tttgagcggc
     6061 tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat
     6121 caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta gctgtctttg
     6181 gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac aaagggatac
     6241 tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat ttattaagtg
     6301 ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct tcattaataa
     6361 aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata gaaacaatta
     6421 tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa catttccgta
     6481 aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt gtcccgattc
     6541 ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga aattttaacg
     6601 ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt
     6661 gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa
     6721 ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc
     6781 catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa
     6841 gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta
     6901 gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg
     6961 ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac
     7021 atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta
     7081 gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa
     7141 gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag
     7201 acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagcg
     7261 acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc
     7321 tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt
     7381 cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa
     7441 tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac
     7501 caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga
     7561 acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa
     7621 ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag
     7681 atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga
     7741 gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac tgagttacca
     7801 aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg taacaaaacc
     7861 actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggagat
     7921 atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta
     7981 gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa
     8041 gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg
     8101 cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa
     8161 acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc
     8221 aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca
     8281 gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt
     8341 ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg
     8401 attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt
     8461 cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc
     8521 gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc
     8581 tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat
     8641 ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt
     8701 ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat
     8761 gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg
     8821 ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct
     8881 acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa
     8941 aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga
     9001 agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa
     9061 aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga
     9121 atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac aattcggagc
     9181 agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa
     9241 caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttagac
     9301 tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt
     9361 cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt gctgaagatg
     9421 aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta tcaaaaagga
     9481 tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt
     9541 ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt ttatgatcca
     9601 aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact ttttggtcaa
     9661 ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat ttttatggat
     9721 ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa tctgacacaa
     9781 gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat ggttttcttt
     9841 acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa
     9901 aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc gattgatcac
     9961 ataaaaggtg tcgaaaatcg caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa
    10021 gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc
    10081 acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt attgaacatc
    10141 atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc acttttcagt
    10201 agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga tccgtttatt
    10261 caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa
    10321 gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga tttatatgga
    10381 acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa acggtatgta
    10441 gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca aggcggggga
    10501 aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag tctacgtgtg
    10561 ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga ttggacgatt
    10621 ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa gaattagatt
    10681 agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt ttttcatatt
    10741 actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca atggtttgat
    10801 aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact tgctctggac
    10861 gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa gctaaaacaa
    10921 gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg acagcggtta
    10981 aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga tctccttcac
    11041 caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa gcaagaggaa
    11101 aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct ttttcagcct
    11161 gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct gcattcgctt
    11221 ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt cctgcttctt
    11281 ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc ggaaagtgga
    11341 tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc accgattgtt
    11401 tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga gcagcttttg
    11461 tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg ccaatcagtt
    11521 cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc ccgctattca
    11581 aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa agattatgtc
    11641 ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa tgtccgattc
    11701 ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat accaaaaagc
    11761 tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt gagactggtt
    11821 tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt
    11881 tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct aaagacactc
    11941 tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga
    12001 aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt catgatgaac
    12061 aagggtgatt tttttattta catagctaag aaagggagag aaaactatga gtttccaaag
    12121 accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga
    12181 aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac cgatttttga
    12241 acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt caaaagagat
    12301 gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag gtacagcacc
    12361 aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga aaccgttcaa
    12421 aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac ggttacgtca
    12481 attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg atgtggagag
    12541 catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc ggctagtcat
    12601 caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga tcgactatct
    12661 tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt
    12721 acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag ctccatctat
    12781 cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt ccatgctgga
    12841 tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag attattacac
    12901 acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag caacgatttg
    12961 cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa cacctggctt
    13021 cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg aagtagttat
    13081 ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa caaatatcga
    13141 agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc gcgacttcat
    13201 gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca aattagtatt
    13261 ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa tggcaagccg
    13321 aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag tctatgatga
    13381 aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat ctattgcgga
    13441 ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt acaaaaaaga
    13501 cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc
    13561 gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg tcgcagtgaa
    13621 tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt aaatcctaaa
    13681 atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa
    13741 accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg tttgaaatat
    13801 cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca
    13861 acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga aacaccttac
    13921 ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg tgtccatgct
    13981 ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct gatgaatgct
    14041 ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt acgtggagac
    14101 cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc agaagaaatc
    14161 caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc
    14221 acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa
    14281 ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa agaagtagaa
    14341 ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa
    14401 ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata tgtaggacaa
    14461 ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa aggtccaatt
    14521 gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc aaaaccaggt
    14581 gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc
    14641 cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa tttcttatgg
    14701 gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt ctctgctcac
    14761 catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga ccctgcaaaa
    14821 gtctatgcag aagcttatga tatcgtcttg aacggttatg aattaggcgg aggttctatc
    14881 cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa
    14941 caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt ccctccacat
    15001 ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga agataatatt
    15061 cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag tgatgcacca
    15121 agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa tatagaagaa
    15181 tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa ccttgattta
    15241 attgaaaaac actttataat gaagccataa catataaagc ggggcttttc catgaaaaaa
    15301 acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc tatcgtttat
    15361 gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat ttattcaagt
    15421 ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat aggattcgaa
    15481 gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt ggcgtggtta
    15541 aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac ctccctttgt
    15601 atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc gttgtttggg
    15661 ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc atcgggcggt
    15721 ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg
    15781 atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg gcaatatatg
    15841 ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa
    15901 caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc
    15961 caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc ttataagcat
    16021 gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct acttagagaa
    16081 gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt ccagatactt
    16141 ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg aaatactgct
    16201 gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca gcaaagaaaa
    16261 actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat ttttttaaat
    16321 ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc tattttcact
    16381 taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa
    16441 acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc
    16501 tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg
    16561 cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg
    16621 ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta
    16681 tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg
    16741 gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg
    16801 gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca
    16861 tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt
    16921 gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt
    16981 cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta
    17041 ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg
    17101 gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc
    17161 tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa
    17221 tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt
    17281 gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat
    17341 tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc
    17401 aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat
    17461 tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta
    17521 tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa
    17581 accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc
    17641 accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa
    17701 aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc
    17761 gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact
    17821 aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc
    17881 aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga
    17941 aaaattaaag gaacaagatt tgttgaaaga cactgttctg attggactag gaacgaatgg
    18001 ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata
    18061 ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga
    18121 acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga
    18181 ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta
    18241 cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac
    18301 aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa
    18361 gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga
    18421 gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc
    18481 ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct
    18541 tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga
    18601 aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata
    18661 atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt
    18721 aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa
    18781 ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg
    18841 caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca
    18901 tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg
    18961 tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg
    19021 gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga
    19081 cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa
    19141 gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag
    19201 ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa
    19261 caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa
    19321 aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc
    19381 gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata
    19441 ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat
    19501 gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga
    19561 gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa
    19621 gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg
    19681 gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca
    19741 gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga
    19801 ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg
    19861 tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga
    19921 atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg
    19981 ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag
    20041 aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa
    20101 ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag
    20161 tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa
    20221 aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa aatagaagac
    20281 aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt tgaggtgatt
    20341 gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa ggttatgact
    20401 tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa gaagaaaaag
    20461 tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct gttgatgccg
    20521 tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg
    20581 ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca tggttcagtt
    20641 attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc gagtttaaaa
    20701 acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta tattttgaag
    20761 atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa atgcaggagc
    20821 tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg ggaagtgtaa
    20881 aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct ggtgaactac
    20941 ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct ttgagacagg
    21001 ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc aaccaaaaaa
    21061 aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg gagatcgaac
    21121 ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat cctgcctttt
    21181 atcagtccgt caatcgggag aattacatct atatctatct cactgataga acgatcatcc
    21241 cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt gaacgggcaa
    21301 acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa
    21361 aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca aaccaagaaa
    21421 aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga atgaaaccga
    21481 aaaa
//
LOCUS       DASJON010000029        20873 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJON010000029 DASJON010000000
VERSION     DASJON010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.029"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..509)
                     /locus_tag="U3989_000825"
     CDS             complement(132..509)
                     /locus_tag="U3989_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714290.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="U3989_000826"
     CDS             765..1493
                     /locus_tag="U3989_000826"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN1714291.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="U3989_000827"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="U3989_000827"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN1714292.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="U3989_000828"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="U3989_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN1714293.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="U3989_000829"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="U3989_000829"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN1714294.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="U3989_000830"
     CDS             4628..6067
                     /locus_tag="U3989_000830"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN1714295.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /locus_tag="U3989_000831"
     CDS             6090..7142
                     /locus_tag="U3989_000831"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN1714296.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="U3989_000832"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="U3989_000832"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN1714297.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="U3989_000833"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="U3989_000833"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN1714298.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="U3989_000834"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="U3989_000834"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN1714299.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="U3989_000835"
     CDS             10661..11575
                     /locus_tag="U3989_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1714300.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="U3989_000836"
     CDS             complement(11708..13273)
                     /locus_tag="U3989_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1714301.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="U3989_000837"
     CDS             complement(13266..14717)
                     /locus_tag="U3989_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1714302.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="U3989_000838"
     CDS             complement(14731..15747)
                     /locus_tag="U3989_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1714303.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="U3989_000839"
     CDS             15967..17055
                     /locus_tag="U3989_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN1714304.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="U3989_000840"
     CDS             17109..18635
                     /locus_tag="U3989_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN1714305.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="U3989_000841"
     CDS             18637..19842
                     /locus_tag="U3989_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1714306.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..>20873
                     /gene="guaC"
                     /locus_tag="U3989_000842"
     CDS             20099..>20873
                     /gene="guaC"
                     /locus_tag="U3989_000842"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN1714307.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSAS"
BASE COUNT         6663 a         3546 c         4753 g         5911 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcgccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cag
//
LOCUS       DASJON010000030        20839 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJON010000030 DASJON010000000
VERSION     DASJON010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.030"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(272..1582)
                     /locus_tag="U3989_000843"
     CDS             complement(272..1582)
                     /locus_tag="U3989_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1714308.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1594..2952)
                     /locus_tag="U3989_000844"
     CDS             complement(1594..2952)
                     /locus_tag="U3989_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1714309.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3154..4722)
                     /locus_tag="U3989_000845"
     CDS             complement(3154..4722)
                     /locus_tag="U3989_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1714310.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4726..5136)
                     /locus_tag="U3989_000846"
     CDS             complement(4726..5136)
                     /locus_tag="U3989_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1714311.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5133..6056)
                     /locus_tag="U3989_000847"
     CDS             complement(5133..6056)
                     /locus_tag="U3989_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN1714312.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6053..6931)
                     /locus_tag="U3989_000848"
     CDS             complement(6053..6931)
                     /locus_tag="U3989_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN1714313.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6918..8255)
                     /locus_tag="U3989_000849"
     CDS             complement(6918..8255)
                     /locus_tag="U3989_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1714314.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8258..9361)
                     /locus_tag="U3989_000850"
     CDS             complement(8258..9361)
                     /locus_tag="U3989_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1714315.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9435..10943)
                     /locus_tag="U3989_000851"
     CDS             complement(9435..10943)
                     /locus_tag="U3989_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1714316.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10909..11601)
                     /locus_tag="U3989_000852"
     CDS             complement(10909..11601)
                     /locus_tag="U3989_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1714317.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11623..12597)
                     /locus_tag="U3989_000853"
     CDS             complement(11623..12597)
                     /locus_tag="U3989_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1714318.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12897..13769
                     /locus_tag="U3989_000854"
     CDS             12897..13769
                     /locus_tag="U3989_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1714319.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14227..16425)
                     /locus_tag="U3989_000855"
     CDS             complement(14227..16425)
                     /locus_tag="U3989_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1714320.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16657..17649
                     /locus_tag="U3989_000856"
     CDS             16657..17649
                     /locus_tag="U3989_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1714321.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17709..19130
                     /locus_tag="U3989_000857"
     CDS             17709..19130
                     /locus_tag="U3989_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN1714322.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19123..20622
                     /locus_tag="U3989_000858"
     CDS             19123..20622
                     /locus_tag="U3989_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN1714323.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20661..>20839
                     /locus_tag="U3989_000859"
     CDS             20661..>20839
                     /locus_tag="U3989_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1714324.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYK"
BASE COUNT         6299 a         4267 c         3570 g         6703 t
ORIGIN      
        1 gtttttcgaa atctttcagc aactcattta tcataactta tcgtttgtta tttgtcaaga
       61 acttttttga agtttttttc gatagaatca agcttcgcaa gtggttcttt ttaacgactt
      121 agttatctta tcacgtctaa aaacatgtgt caactctttt ttggaatttt cataaaatgt
      181 ttcaatgaag cgcgacaacg ttgtttaata tatcattaaa aaaaataaag gtcaagctaa
      241 aatattattt aagcttgacc ttattataat ttcaacgatt gtcaaaagta ttgtttgcag
      301 ctgtgttgcc agtttctata gataaatcac agcttttgaa acataagttt tgtactgctt
      361 cgattcgccc gaattctttt gcagttattt ggcaatgaga aaagcagaca tcttctatag
      421 gcttttcagg aaatgctact aaatcaaaag ctcgggaagg aagtgagtaa cctggagaaa
      481 ggcatgcctg tacgtttttg atgtgtatgt ctttgacttt cgtcatttgc tttgattcag
      541 gtatttcttc agccactgct tgccaacttt ctggcatttg agcaaggtca ttgctatttt
      601 ttctattgta ttcattatgc cagtccatta tccaagaaaa aggaaattgt acatcaatca
      661 tattcagatt ttctaccctg atattttcga tcactccccc tctttctttt gaagatttca
      721 ttcgaatacc acagtctgac tgaaaaaaat cgatatcgtg aatatatacg tcactaactc
      781 cagcactaac ttcgctacca atagttacac cgtatcctga acggatttta catctagcaa
      841 cctcgattct tgatgatttt cgattgactc ttgcaccatt gccatctctg ccagacttaa
      901 tagcaataca atcatcacca caggccaaat cacattcata gacacgcaca tttgttgaag
      961 aatcaatatc aatcccatct gtacttggtc catcattgtt gcggatgatt actttttcta
     1021 ctaatacatc gttagaataa gtgatttgca agttccaaaa acctgatttc tgaaaaatca
     1081 aatcacgaac aacaacatgt tctgcatgat acagtaaaca agcgcgtgga cgtttaatag
     1141 cataatccgc aatccaacga agtccctttt gatcgtattc agctcttgtc ccgctttttt
     1201 gatcttttcc ccaatacagc tcccaccaat ggtctccttg accgtcgatt attccttttc
     1261 cttctataag aatatctttc gccgagagaa cattcaaaat cgctgctggc catttcattt
     1321 ctacgccggc tactcgtgta tcgatttctg gaaatgcttc gatatctgtt ctccccttga
     1381 ggactgcacc ttcttcaaag ataagatgac tcttatcttt taaaaaaagg gctccgacta
     1441 aataaatgcc ttttggaata aatacaggta agtgttcttt cgcaccttga tcgattgctt
     1501 tttgcagtat tattgtatca tcttgttgag tgtcgtttgt tttaaagttc aaagcattga
     1561 taatcccttt ttttgtccgc aatacgtttc tccctatctt tcattgattt ttttcaattc
     1621 ttcaccaata aatacagttt ttgaacgatt cagtgggtaa cctactagca aaatgattgc
     1681 ggatacaaat aagcagaatg ctggtaacaa gtttgccatc gtataaatcc tttcttgtac
     1741 taaaactgat tggatggctc cacctgttgt agacgattgg taaccaatga gtgccagcat
     1801 aaatccgcct accgctcctg cacatgcttg tcctactttt ctcgcaaaag aatttactcc
     1861 atatactgtt ccatcttctc ttaagcctgt cttatattga tgataatcaa tcacgtcagt
     1921 aatgaatgcc cagaccatca aattgaataa accagatcct aaagtagcga taaataaaag
     1981 aatcagatat acccatgcat ttgttatatg aaggaaatac atcagcaagt acatacatga
     2041 agaagcaagt aacgcaaata cgcttgcttc ctttttacca aataattttg tcagctttgt
     2101 agcaaatggt gctaaaacta ctactgttcc gtaagtaaat aaaagagcaa cagacattgc
     2161 ttgtttatta tggaagtaat cattgaataa ataagtcata ttcgtcccag ctaaaatctg
     2221 attgatcaca atgaaaatgt caacaatgac taaaataatc agtgctcgat tagctaacaa
     2281 accttttacc aactgagaag cagatacttt ttcgtttttg acaactctta ctctttcagt
     2341 agtcaattta caagttaaca tatatgcgac aaatgctatt gcagcacatc cgattgaaat
     2401 ccaaaagaaa tgattccctg aaacgacttg ttgtccactc ttgttatggg tatacattaa
     2461 aatcggaatg aagaatcctg tagttgatcc gccaatcgca gaaccaatgc ttcggaacgt
     2521 ggacaacgag gtgcggtcat taggatttga gctaattgct gctgccattg ctccatacgg
     2581 gatattgatt gttgagagaa aaatcccata aacaatgtat gaaacaaaaa tataagcaat
     2641 cttcaccgtc attggaagat gttggacgaa tggcaaaaac aaaatgacag tcactaagca
     2701 aaatggatac ttcatttttc tgatccaagg aataaatcgg ccagtttctg ttaactctga
     2761 gacatcacat aatcttccaa ccatcacatc agcaaacgca tccaaaattc tcgaaatcaa
     2821 gaataacgta cctacgattg cccctgaaac gccaagaaca ttcgtataat aaatcatcaa
     2881 gaaactattg actaaaccta aaataaaaca gttacctaga tcaccgaaca tgtagcctat
     2941 cttatcggaa atcccaaatt cctttttttc ttgtttttat attcacaatg ttggcttttt
     3001 attgcattat cttgacagat aaaaaacaaa aaataatcta aaaacattga tgcataagat
     3061 tgataacttt ttttacataa caatatatac aaacaaaaaa attcactaac tagtgaaaat
     3121 aagttagtga acctcattta ttaaagaatt aaattacaga tgatcttttc catcatactt
     3181 gaaaaactca aaatcagcat aacttcctct ttttttattt agatcatgca ctgctatccc
     3241 gataaaattt ccagtaaacc ctcctgagag tatacacatg tttttttgcg gacctacttt
     3301 cttccattct tcagttgata cgtctttcaa atgaaactgt gctgtctctt tctttacagt
     3361 gactttcaaa aaagtcttct tttctgtgag tggaatcgtc caatcttcta tttgtgctac
     3421 tccgttttct gattctatca tacgtaggca ttttcccttg tcctcattcc aagtaacata
     3481 acaataaata taattggatt catttaagta cagtaccagt ccagccatct gattaaaatt
     3541 attaggcatg tattcaaatg ctgtctccgc atcaaaagta aaatccgttt gccgaatagc
     3601 taacaaatga tgttcgaagg tagattgaag agattcaccg gaaatcagac gtaaaaaacc
     3661 tggtctacat tttgtatcac accaatcagc atttggcaaa atgcgtctgg cattccagtt
     3721 cttctttagc tctccttcaa atgtatccat aaactgatga tctttttctt gttgtacctc
     3781 ttcatttgtc tcaatcatta tttcttccat cggagttgtc tggctatctt taagtcttaa
     3841 ccaatcgtcg gacgtccaat acacttcttg aatagcggtt tctcttccca aaatagccgc
     3901 accgtttaat ggtctggtac aaagatatgt catgtaccat cttcctgacg gggtttgtac
     3961 taagctccca tgtcctgtac attgcaatgt agaagcaggt ttgtcactag ctgtcagcat
     4021 aggaaaacca ggatcaagct cgtaaggacc agtaatattc tttgctcggc aaactgtaac
     4081 tgaatgtcct cttcctgtac cgccttctgc cgtcatcaaa taataatacc cgttacgaaa
     4141 atacaaatgt ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt tatagatggg
     4201 accatcaagc ttcttcttct ctgcatcata ttcttgtaat acgataccgc tggatttatt
     4261 cggtgtgtcc agacgataat cccatagttc attgacaagc catttttttc ccgtttcaag
     4321 atcatggaac aaagaaggat caaagccgtt actatttaaa taaattggtt cggaccaagg
     4381 acctgtgatc tcagatgcag tgatcaaata attgtggaca tctttaaacg gacggcctgc
     4441 acttttgaca tctgtgtaaa caagataaaa caatccatca gcataactga tctgtggcgc
     4501 ccatatactt ccatttgtag gatttccttg caaagatact tgagtggtca aaatatccgt
     4561 tgtatgtgac cagttgacta aatttttcga ctcatatact cggattcctg gaagccattc
     4621 aaaagaggag acaataatgt aataccgctc tttcactctt aatatattag gatctggatt
     4681 gaatcctctt aaaataggat ttttcacttt gatagtctgc atgattcagg ctccttttct
     4741 cattcttact cgatataaac tgaatgcccc agctgcgaaa taaaaaaatg caaaaggaaa
     4801 cgcaataaac tgagtgccaa tcaacaatat gaatcctgtt ataaaataca aaccagtggc
     4861 agttgttaag tattttcctt tagcagtcaa aaacaatgct actgctaaaa aagcaagtga
     4921 aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc
     4981 taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc
     5041 agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc
     5101 gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa
     5161 tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg
     5221 acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca
     5281 cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag
     5341 acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt
     5401 tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg
     5461 atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca
     5521 atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa
     5581 aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa
     5641 ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc
     5701 tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca
     5761 ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga
     5821 tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta
     5881 aaattggata atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc
     5941 tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa
     6001 tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat
     6061 gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg
     6121 ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc
     6181 cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc
     6241 taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata
     6301 gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtattt
     6361 cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca
     6421 cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg
     6481 tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc
     6541 ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc
     6601 gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt
     6661 ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg
     6721 aaaaatctcc gccgtgagaa tgcaggtaaa ggaccggcgg aaacggtcct tctttttctg
     6781 gataagcata gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa
     6841 gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag
     6901 atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca
     6961 tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct
     7021 aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc
     7081 gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga
     7141 acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt
     7201 atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg
     7261 atattcgaaa aatggatcct tctgaatgct ggtgtccgtt catctatcgg ataagccttt
     7321 ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag attcagtata
     7381 aatggacaca tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg
     7441 cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt
     7501 gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt
     7561 ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta
     7621 tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta
     7681 atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg
     7741 ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa
     7801 cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc
     7861 atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat
     7921 ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga
     7981 gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca
     8041 ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg
     8101 acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc
     8161 ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca
     8221 tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca
     8281 atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt
     8341 agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt
     8401 tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat
     8461 gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt
     8521 tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt
     8581 ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg
     8641 cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta
     8701 attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat
     8761 gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa
     8821 tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa
     8881 catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac
     8941 ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc
     9001 cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat
     9061 atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt
     9121 cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt
     9181 cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt
     9241 ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt
     9301 catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca
     9361 ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt
     9421 cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt
     9481 cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc
     9541 tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact
     9601 tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt
     9661 tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc
     9721 acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg
     9781 ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc
     9841 atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat
     9901 tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat accatttcct
     9961 tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat
    10021 tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca
    10081 gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact
    10141 gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc
    10201 atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca
    10261 tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc
    10321 gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta
    10381 aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta
    10441 tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg
    10501 ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa
    10561 tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg
    10621 cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca
    10681 gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc
    10741 agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca
    10801 tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg
    10861 ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat
    10921 tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt
    10981 ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc
    11041 tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat
    11101 tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg
    11161 aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc
    11221 ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat
    11281 atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat
    11341 cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata
    11401 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
    11461 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
    11521 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
    11581 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
    11641 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
    11701 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
    11761 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
    11821 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
    11881 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
    11941 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga
    12001 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
    12061 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
    12121 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
    12181 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
    12241 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa
    12301 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
    12361 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
    12421 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
    12481 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
    12541 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
    12601 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
    12661 ttacgtatat aatcattttt accgtaagta aaaacgctta catagcaatg atttttgtgc
    12721 tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
    12781 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
    12841 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
    12901 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
    12961 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
    13021 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
    13081 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
    13141 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
    13201 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
    13261 gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact
    13321 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
    13381 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
    13441 gtgtattttt caaaattatg ctgccattag cgttaccgtc tattgctact atctcgcttt
    13501 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
    13561 ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag
    13621 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
    13681 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
    13741 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
    13801 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
    13861 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
    13921 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
    13981 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
    14041 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
    14101 gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
    14161 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
    14221 cttgattcac aaatattttt ttcgatattc tcccggcgta atcgattcct tctttttaaa
    14281 gaaacgaata aaattttgcg gatttcgata ttgcaatctt tctgcaatct cttttacgga
    14341 gaggttagtc tctttcaacc atttttttgc tacctctaaa cgatgatttt gaacaaaatc
    14401 accaaatttt tctcctgttt cctttttaaa tacattactt aaatagttag ggttataatg
    14461 caacctatca gcgatcacat ctaaagacag atcacgatca tattctgtgt agacgatatg
    14521 cataatattc tccgacaaat ttttaaattc atgatcagtg attgcttgag agcattctgt
    14581 gattggcaga actaattgtt ctaaaacaag tttttctagt tctttgggat gataattgtt
    14641 cagtgctttt acataaattt gtttcatgtt atcaaagttt tttgaatcta ctcccaataa
    14701 ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa actacttccc gactcaaagg
    14761 atttttattt tgtgcaaaca atgcatcaat caattcatgt actaaattct tcacttcctg
    14821 atttccagaa cgaatggctt caaacaatcg attttgttct tctatcggat atctaataag
    14881 tgttttttca tgagcagcag gaacgatttc ctgataaaaa ataattgaac ttggaccagt
    14941 atttacacga taatacaggg cttcttttgc ccgatcaacc gattgcttgc tttcattcag
    15001 cgtttcgaac cggttactga tgcctatact gatagttaaa ttcaagtatt cttttacacg
    15061 tttttgaatg agtcggcaat actccatgac ttttttgctt gcttctgcat catctttatc
    15121 cattcgataa atagtggctt gcatttcttc attcaaaacg atcggcagca ttcgattttc
    15181 ctttggcaca atttccgtga tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc
    15241 accgtgacta tcttctaaca gatcgatttg aaccaaagct gtataatagc agtcattttg
    15301 agaactataa ccaaattgct gcaaccgctg atctagttct cgcctttcta ctcgatttct
    15361 aaataaacta agtacaaata gcgtttccag ctgtggcttt tgggtaacta agtgtgcact
    15421 taacgcttct ttttctccta tgatccgact gactgattga gcgaccaaat ctagttcttt
    15481 cccactgaat ccttttgatt taagattcag acgttcctgt atttgagaaa tcggacgggc
    15541 aaatctgtcc gaaaataagt aggaaagaag accaatgagt cctaccagtg ttaaagacac
    15601 tgcaactagc ccgattctca gattgcggat cattgcgcca attgcactag agtcaacttc
    15661 taatctgtaa atccaattat tataatctga tttcagtact atatacgttt tatctttttc
    15721 tcttatcact ttcagctgat tgtaatctag atccacgtcc gttaatacag ttggttgctc
    15781 tttatttttt actgtctgag tagtaaaaag gttgccaact tcgttttcaa tagaaagtat
    15841 gtcttctcca ctttcaccaa caacttgttc tattgtatgt ttctctatcg tagcaattcc
    15901 caatgcatat ttttcttttt caaacattgg caaagaaatc accatttcga ttcctttgtt
    15961 tttcttttgc caaaataaat tatttttgtt atttatgtac ttctgttgat acttttcaat
    16021 ttcctcttcg gtcagttgac tcaacgatcc attgattact ccccatttac cagccaaact
    16081 gactaaatca taattattat catccataac aatcgtttct ataaaattta gttctttcct
    16141 gatttcagaa taagtggagt aatctgtata tgtcagcgga tgcttgaata tgtccgagaa
    16201 atttttggtc gaaccatacg tagaaaacga atattcaatc gttctaattt tccattcaac
    16261 gttagtttta acttgcctga gcaaattctt tttgtctgtt aaataactcg attcgatcga
    16321 ttcataggta ttctggtaaa tgaataaact aaatcctatg acaagtacgg ctcctaataa
    16381 aaacatcggt aaaaatattt tataaaatac tgtacttttc ttcattttct tcctctcctc
    16441 cttttaaaat aaaaggcttt catttttctg taaaattgat actcatctaa ttaaatgagc
    16501 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
    16561 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
    16621 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatgtcgct
    16681 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
    16741 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
    16801 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
    16861 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
    16921 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
    16981 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
    17041 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
    17101 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
    17161 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
    17221 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
    17281 ccagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
    17341 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
    17401 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
    17461 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
    17521 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
    17581 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
    17641 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
    17701 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
    17761 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
    17821 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
    17881 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
    17941 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
    18001 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
    18061 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
    18121 cacagagcct cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
    18181 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
    18241 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
    18301 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
    18361 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
    18421 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
    18481 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
    18541 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
    18601 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
    18661 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
    18721 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
    18781 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
    18841 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
    18901 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
    18961 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
    19021 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
    19081 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
    19141 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
    19201 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
    19261 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
    19321 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
    19381 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
    19441 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
    19501 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
    19561 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
    19621 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
    19681 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
    19741 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
    19801 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
    19861 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
    19921 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
    19981 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
    20041 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
    20101 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
    20161 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
    20221 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
    20281 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
    20341 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
    20401 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
    20461 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
    20521 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
    20581 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
    20641 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
    20701 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
    20761 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
    20821 gaggcgatca ttacaaatg
//
LOCUS       DASJON010000031        19835 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJON010000031 DASJON010000000
VERSION     DASJON010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.031"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..3158)
                     /gene="carB"
                     /locus_tag="U3989_000860"
     CDS             complement(<1..3158)
                     /gene="carB"
                     /locus_tag="U3989_000860"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN1714325.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMES"
     gene            complement(3163..4239)
                     /gene="carA"
                     /locus_tag="U3989_000861"
     CDS             complement(3163..4239)
                     /gene="carA"
                     /locus_tag="U3989_000861"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN1714326.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(4244..5524)
                     /locus_tag="U3989_000862"
     CDS             complement(4244..5524)
                     /locus_tag="U3989_000862"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN1714327.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(5543..6469)
                     /locus_tag="U3989_000863"
     CDS             complement(5543..6469)
                     /locus_tag="U3989_000863"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN1714328.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(6482..7765)
                     /locus_tag="U3989_000864"
     CDS             complement(6482..7765)
                     /locus_tag="U3989_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN1714329.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(7779..8318)
                     /gene="pyrR"
                     /locus_tag="U3989_000865"
     CDS             complement(7779..8318)
                     /gene="pyrR"
                     /locus_tag="U3989_000865"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN1714330.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(8553..9464)
                     /locus_tag="U3989_000866"
     CDS             complement(8553..9464)
                     /locus_tag="U3989_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1714331.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(9461..9925)
                     /locus_tag="U3989_000867"
     CDS             complement(9461..9925)
                     /locus_tag="U3989_000867"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN1714332.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(9930..10628)
                     /locus_tag="U3989_000868"
     CDS             complement(9930..10628)
                     /locus_tag="U3989_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1714333.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(10615..11001)
                     /locus_tag="U3989_000869"
     CDS             complement(10615..11001)
                     /locus_tag="U3989_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714334.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(11148..12818)
                     /locus_tag="U3989_000870"
     CDS             complement(11148..12818)
                     /locus_tag="U3989_000870"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN1714335.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            13001..13234
                     /locus_tag="U3989_000871"
     CDS             13001..13234
                     /locus_tag="U3989_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN1714336.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(13333..13764)
                     /locus_tag="U3989_000872"
     CDS             complement(13333..13764)
                     /locus_tag="U3989_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN1714337.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            13843..14256
                     /locus_tag="U3989_000873"
     CDS             13843..14256
                     /locus_tag="U3989_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN1714338.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(14294..15442)
                     /locus_tag="U3989_000874"
     CDS             complement(14294..15442)
                     /locus_tag="U3989_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1714339.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(15564..16160)
                     /locus_tag="U3989_000875"
     CDS             complement(15564..16160)
                     /locus_tag="U3989_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1714340.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(16360..19002)
                     /gene="alaS"
                     /locus_tag="U3989_000876"
     CDS             complement(16360..19002)
                     /gene="alaS"
                     /locus_tag="U3989_000876"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN1714341.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19390..19821
                     /locus_tag="U3989_000877"
     CDS             19390..19821
                     /locus_tag="U3989_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1714342.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
BASE COUNT         5544 a         4364 c         3508 g         6419 t
ORIGIN      
        1 gattccatca ctcgaacgat cgcatctgct gtgtctaaag aagtaaacaa tggaatacca
       61 tgttcaactg cttctcttcg gatgatgaaa ccgtctttag aagcatttcc ccgatcttta
      121 tcaatcgtat tgacaaccac ttgtgtttta ccatctctta ttaagtcgac tacatttttt
      181 tctgctgctt cctcagaaat tttcgctact gtttcaacga ccagaccatt tttttcgaaa
      241 taagcagctg tatgtttcgt tcctatcaga ctatagccaa tttctgtaaa acgtttagct
      301 aattctagtg cttcttcttt tgtttcgtcc gcaatcgtaa ataatacggc tccatattca
      361 ggcaagtgta atccagaagc ttcgaatgcc ttgtacaaag ctttgtctaa ttgtctgtca
      421 gatcccatta cttctccagt cgatttcatt tcaggcccta agtaagtatc aactttctgc
      481 aatttcgtaa aagaaaagac aggtgcttta acatgaactt cttttgtttc tggataaagt
      541 ccgttttcat aaccaagtgt ctttagtttc tctcccaaaa tagctttcgt cgcaatttgt
      601 gccattggaa ttcctgtcac cttacttaag aatggaacag tccgacttgc tcgaggattg
      661 acttcgatca cataaacctc attttcatgg atgacaaact ggatattcat catcccgaca
      721 caattcaaac ctaatgctaa gcggatcgtg taatcttcga tcgttttctt gatcgattca
      781 gataatgttt gaggtggata cacagccatt gaatcccctg aatggacacc agcacgctca
      841 atatgctcca tgatccctgg gatgagtact gtttcaccgt cacaaatcgc atccacttcg
      901 cattcaccac caagcaagta acggtccact agtactggat gttctggaga tgctttcaca
      961 gcatgttcca tgtaatcttc taaatctcgc tggttttcaa cgatttccat cgcacgtccg
     1021 cctaatacat agcttggacg aacaagaact ggataaccaa ttctcgaagc gataaccacg
     1081 gcttcttctt tacttgtagc agtatcccct aaaggttgag gaatatccaa ctctttcagc
     1141 gcttgctcga ataaatcacg attttccgca cgatccaagt cttcgatcgt tgtccctaag
     1201 atttttacac cagcagcagt caaaggttcg gctaagttga ttgctgtctg accaccaaat
     1261 tgaacgatta ctcctatcgg ctgttctagc tcgatcacat tcatcacatc ttcgaaggtc
     1321 aatggctcaa aatataattt atccgaaata gaaaagtcag tcgaaactgt ttcaggattg
     1381 ctgttcatga tgattgcttc gtatcctgcc tgctggattg ctttgacaga atgcacagtc
     1441 gcatagtcaa attcaacccc ttgtccgata cggataggac cagatcccag tactagaatt
     1501 gagggctttt ctgatcgtga actttcattt tcatattcat aggaactata aaaataaggt
     1561 gtttttgatt cgaattccgc tgcacaagtg tcaaccatct tatatacagg aatcaaatca
     1621 ttttcttttc tcatctggcg aacggcttct tcgtttattt cccacagctc cgccactttt
     1681 ttgtctgtaa aaccgttttg tttcgctgtt ttcaatatct tgtcatccaa cggctgctgt
     1741 ttcaactgct gttcgatttc tacgatatgc aacagtttat ctaaaaagaa taaatcgatt
     1801 tttgtcaaat ccgctaattc ttctatggtg taccctcttc taatagcttc agctacataa
     1861 aacaggcgat catcttgtgc tcgaaccact ttttggatta ggtcatcgtc cgtgatgtct
     1921 gccaactcag gaagttcgat atggtgtgta ccgatttcta aagaacgcac agctttcaat
     1981 aaagactctt cgatattccg accgatagcc atcacttcac cagttgcttt catctgcgtg
     2041 cctaaaagcc gttctccgtt ttcaaactta tcaaacggcc agcgaggaat tttagctaca
     2101 acataatcta atgcaggttc aaattctgca aaagtcgttc ctgtaacagg atttttcatt
     2161 tcgtctaatg ttaatccaag cgctatttta gcagctagct tagcgatcgg atagccagtc
     2221 gccttacttg ctaacgcaga tgaacgagaa acacgcggat tgacttcaat cacataataa
     2281 ttaaaactat gtggatcaag agcgagttgg acattacagc ctccctcgat cttaagtgct
     2341 cgaataatcg aaagagaagc atcacgaagc atttgatatt catggtcaga caatgtttga
     2401 ctaggggcaa atacgataga atcacctgta tggataccca ccgggtcaaa attttccata
     2461 ttgcagacaa cgattgcatt atcagccgaa tctctcatca cttcatattc aatttcttta
     2521 aaacctgcaa tacttcgttc aatcaaacat tgggtagcgg gggaaagact taatccattt
     2581 tctgatacga tacgtaattc ttcttcattt tcacacatac ctccaccagt tccgcctaaa
     2641 gtaaaggctg gacgaacgat gacaggatag ccgattttat ttgcaaaatc tacagcttct
     2701 tctactgtat tgacgatttc agactcgggg attggctgat tcaattcttt catcaactgt
     2761 ttgaatagat cacgatcttc tgcttgatcg atcgcagaaa gctttgttcc tagaagttct
     2821 atgttcaatt cttctaaaat acctgatgct gccagctcca ttgccatatt gaggcctgtc
     2881 tgtccgccta aagttggaag gagagcatct ggacgctctt ttcgaagaat ccttgataca
     2941 aattcaagtg tgatcggttc gatatatact tgatctgcca cttctttatc cgtcatgatc
     3001 gtcgcaggat tagaatttac taaaaccact tcatatcctt cttcttttaa ggcaaggcag
     3061 gcttgtgttc ctgcgtagtc aaattctgcg gcttgaccaa tgatgatcgg accagatcca
     3121 atcaccataa ttttcttgat atctgtacgt ttcggcatct gtttactgct cctttcctgc
     3181 gtccatcatt tccataaatt catcaaataa atgtagtgca tcatgcggac caggggcagc
     3241 atctggatga aactgcacac tgaaagcggg ataatctctg tgtttgactc cttctactgt
     3301 tccgtcattt acttctacat gagttacgat gagtttttct ggatcaatcg tggcttcatc
     3361 gactgcatag ccatgatttt gagaagtaaa atcaattctt ccggtcgcta cttctcgtac
     3421 cgggtgattt aatcctcgat gtccgaattt cattttatag gtatctgcac cgttggctaa
     3481 agcaaataac tggtgaccga gacaaatacc aaaaatcggt acttttcctt ggattttttt
     3541 gatcatttcg attgcttctg gcacatcttt tggatcacca ggtccattcg tcagcatgac
     3601 tccgtctggt gatagcgcta aaatcgtctc ggcatctgtg ttatagggaa gtactgtgag
     3661 gttacagttt cttttactca attctcgtaa aatactatgt ttcaagccga agtcaatgac
     3721 aatgactttc cgacctgttc ctggacttgg ataaggtttg ttagttgaga cttgctgcac
     3781 ttgattggtt gctagaacgg ttgctttcag ctgatcaaat gcatgttcga aggaatctcc
     3841 agcatcaact atactagctt tcatcgttcc gacttcacgg attttgcgag taagggcgcg
     3901 cgtatcgata cctgatattc caggaattcc tttttgtttc aaaaatgaat ctagcgtcat
     3961 ttggtttcgc cagtttgaag ctacacgtgc atgttctttt actactactc ctttgcatgt
     4021 tggagaaatc gattcataat catctctatt taccccataa ttacccacca taggataggt
     4081 aaatgtaatg atttgaccgt taaaactttg atcagtgata gtttcttgat aaccagtcat
     4141 acttgttgta aaaacgattt ctccgaagac actagcatct gcaccaaaag cctttccttc
     4201 gaaaactgtt ccatcttcta atataagcaa acgttccata cttttattct cccttctgcc
     4261 acacaagttt tccgttgaca aaagtcattt gtgtttcgcc ttttactttc cagccgataa
     4321 aaggtgtatt ctcccctttt gataaaaagt cttctttatc gatcgtgcaa gtttgtgtaa
     4381 tatcaaaaac tgctacatca gcgggggcac caaccgttag cgttcctgca ttcaaaccaa
     4441 aaatctcagc cggtttaact gccaaccaat tgatcacttg ttccaacgtg aatcgaccag
     4501 tttctacaaa atgagtatag atcagttgaa acgctgtttc gcttcctacg ataccaaatg
     4561 gtgatttcat gaaactttgg cttttttctt ctaatccatg gggggcatgg tctgtagcga
     4621 tgcaatcgat cgtaccgtca agcaagcctt cgatcaaagc ttctctgtcc tcccgtcctc
     4681 ttagcggcgg gttcattttc caaaagccga aatcttcggg aatatcttcg tcgatcaaaa
     4741 taagatgatg gggtgaaact tctgctgtta cgtggattcc tgcttttttt gcgtctctga
     4801 ttacacggac actttcttta gtagaaacat ggcaaacatg gtaatgaaca ccagttgctt
     4861 cagctagcaa cagatctcta gcaatttgag aagactcggt tacgcttaag atcccgggaa
     4921 gaccaagttc ttctgccttc ttgccagcgt gcataacgcc gccaaataat aaagattcat
     4981 cttctgtatg ggctactagg gctttattgt tttttgcagc ttctttcata gccagataca
     5041 tcgtcccagc tgtttggaca cctacaccgt catttgtgaa agcaaaagca ccttcttcaa
     5101 tcagtgcttc ttgatcaact aatttctcac ttcgcagatt ttctgtgatt ggcgcatact
     5161 gcaatacctt cacaactgca tcttttctaa tgatgtcata tacttgtctc agcttttctg
     5221 ccgtatcagg tacaggattt aaattcggca tcgcgcatac agtagtgaaa ccgccacgag
     5281 cagctgagcg agtcccagct tcgatcgtct ccttgtatgt aaatcctggc tctctcaaat
     5341 gaacatggac gtccactaaa ccaggtgtga tcaattgtcc ctttgcatca aacacttcat
     5401 caaattcggc ttctgagaat cctgtaccga ttgctttgat tcgcccatct tctatccaaa
     5461 tttctgctgg tgtcgtcata ttttttcgtg tattgatctg accgttttta atgagtgttt
     5521 tcattttttc atcctcctat aattacgctt ttccatttag cactgcttct aagattgcca
     5581 ttctgacgaa tactccattg ctcatttgct gaacgatccg cgactgtaat ccttccacta
     5641 atgaatcagc taattctaca tcacggttaa ctggtgctgg gtgcataatg attgcatgtt
     5701 tttgcaactt cttcgcacgc tcaactgtta acccatgttc ctgatggtag ccttcttttg
     5761 agaaactttc ttttccatca tggcgttcgt gttgcacccg aagcatcatc atgacatcaa
     5821 cttgatctag tagttcatcc aatggcatat aatgtccgta cacttcgaat tcatcgtcgt
     5881 accattctct aggacctgag aagaatactt gtgcgcctag acgttttaac atctgcatat
     5941 tggatttagc tacgcgagag tgagtgatat cacctacgat ggcaactttc agaccttcaa
     6001 aatgaccaaa ttcttcataa atggtcatca aatcaagcaa gcattgggtt ggatgctgtc
     6061 cgcttccgtc tcctccattg ataatcgaac actggattgt tttactttgt atcagttcgt
     6121 cataatagtt ctcatcacca tgacggataa ccgctacgtc tacgcctaat gctgacatcg
     6181 tcagtactgt atcgtatagt gtttcccctt tttggacaga gcttgtgctt gcttcaaatt
     6241 caatcacttc tagccctagt tttttctccg ctacatcgaa gcttttatgt gtgcgagtac
     6301 tattttcgaa aaagaggttt gtagcaaagt actggcgctt ttgcggagtc caattagcac
     6361 cttgcttgaa ttcctgccct ctgcggatca agcccataac ttcttgatca gtaagtgcct
     6421 caacggtcaa taaatgcttt aagctgatac gttctgattt gatgatcata atgtttcctc
     6481 cttagttttc ttcgcttcgt gaagtttctg gtaagataag atttaaaatg atgccaagta
     6541 cagttgctaa tgccattgcc gacaatgtaa atgtgcctac ttcaaagacg agtccgccaa
     6601 ttcctactac taagataact gaagcaatca acagattttt ctttttatca aaatcgattt
     6661 tgttctcgat taaaatcttc attccacttg cggcaattac tccaaataag ataaaactga
     6721 ttcctgagat gactggtcca gggatactta aaatcaaagc gcttaatttt cctacgaatc
     6781 ctaaaatgac tgcaaatact gcagctccac cgatcacaaa cacgctatgg acacgtgtga
     6841 tagccagaac accaatgttt tcaccgtaac ttgttacagg aggacctccg acaaaggctg
     6901 ccacgatttg tgctaatcca tcacctgtca acgtcttatg aagacccggt tcttcaaaga
     6961 aattacgttt agttagttta ttcaatacca taagatggcc gatatgctct gtcatcgtca
     7021 caaaagcgat gggtgccatt gttgtgattg ctccaagata tagctttggt tcatattgta
     7081 caaagggaat ctcaaagtta ggaacagcaa accaggcagc gtctacgatt ggcttagtat
     7141 ccacgattcc aaacagcaag gctacgatgt atccgctaat aatacctagt aaaatcggaa
     7201 tcaatccgag aaaacctctt agccacatgt tgaagaaaat tgttaatcct aatgtgatca
     7261 gcgctacagc aatatacttg aaatcatacg tgccctgatt atacatagca tttgttgctg
     7321 catttgccgc aagtcccaac ccgataacca tgatgactgg accgacgacg attggaggca
     7381 agattttatc cagccatgct gaaccgatct ttttgatgat cagcgcaaca atcaaataga
     7441 ctaatccagt tgtgactgct ccttgtgcaa tcgctggata accatctgtt ttcatcaaaa
     7501 gctgcatcgc tgcaatgaaa gcgaagctgc ttcctaagta cgcaggtatc tttcctttag
     7561 tagtaaataa gtacaccaat gtacctagtc ctgaactgac tagcgcaata cctggatcga
     7621 tacctactaa gatggggact aacacggttg ctccgaacat cgtgaacaag tgttgtaaac
     7681 ttaatcctac ccaatgtcct ggggttgggc gatcgtggat atctaacacc acgtcatcat
     7741 tatgaaatcc ttttttctcc gacatgtttc tctctcctct aatcttcctc tttcaaaatc
     7801 atgatgcgat cttgtccatc aagctcttgc atctctacta aaatctcttc cgcccgagat
     7861 gttggaatgt tcttcccaac ataatctgca cggattggca attctcgatg cccacgatcg
     7921 accaggactg ctaatgaaat ttttcttggt ctgccaaaat ccattactgc gtccattgct
     7981 gctcgaatcg ttcgtcctgt atacaaaaca tcatcaatca aaataacttc ttttccttct
     8041 aaggaaactg ggatgtcaga agaatgaagt tccggttctt ctggattttc ttttttgtca
     8101 tctcggtata aagtgatatc cagttctcct acaggaatat cgatatcttc tagctgcttc
     8161 aggcgttcgg caattcttga tgcaatgtag attcctcgtg ttttgatacc taccaatacg
     8221 atatcttgga tactatggtt tctctcgata atctcataag taattcgagt caatgctcgc
     8281 ttcatggtta cctgatctac tacttctttt gcttgcattt tcattccccc tacttttttg
     8341 cataaaaaaa ctcctgccct aagaggacag gaggaatgtt tagactgagc gtatactgat
     8401 acactgatag ctatactatg tcaatgagca atctacttat ggcgcaccat acgtatcctt
     8461 gctcccaccc ttcttagcct cacgggacta aacttaaagg aattctatta agttttatgc
     8521 tttcgttgtt aattactata ccgttatcta aattaaaatg caatgttttt tcttaatttt
     8581 tctaaagttt tttcaaaaac ttctggaaga ggtgcttcaa agatcatctt ttcgtttgta
     8641 gtcgggtgcg taaaacctaa catttgcgca tggagaaact gaccattgcc tttcaaagtt
     8701 ttcttcggtc cgtatactgg atctccagct aacggataac cgatatactt catatgcaca
     8761 cggatctggt gtgttcttcc ggtttctagt tttaattcaa ccagagtaaa tccttcgaaa
     8821 cgttccaata cagtaaaatg tgtgacagcc gacttcccat cttctcttat cgcctgcatt
     8881 ttccggtttt ccttcgaacg gccgattggc gcattgatgg ttcctttttc gtgaggaatc
     8941 tctccatgga ctaaagcaac atatttgcgc aaagaggtct tatcttttaa ttgctgagct
     9001 aatgattcgt gtgcctggtc atttttggca accattagca gtccggaagt atctttgtcg
     9061 attcggtgaa caatacctgg acgaataacg ccgttgatcg tagaaagatc cttgatatgg
     9121 tacatcagcg cattgaccat tgtgccatgc gtatgacctg ctgaaggatg aacgaccatt
     9181 ccttgaggtt tgtttatcac cgctacatct tcatcttcgt aaacgatcgt caacgggata
     9241 ttttctggga ccacatctaa tgtttctggt tcggggatca agacagtaat ctcgtctcct
     9301 tgttttactt tgtaatttgc tttgactggc tgtccattga cagaaaccgc tccatttttc
     9361 agccacagct gaatttgcga acgactgtaa tcggtgaata aattagtcaa tactttatcg
     9421 atcctaccag attcatggtg aatcgtcgtt ttaagttctg tcattttttt ctttgatcct
     9481 tttcatctaa aataatataa attaacatac aaatcactcc acatacaagt gtcacatctg
     9541 ctacgttaaa aatcggaaag ctcataaaat ctgtctggaa catatctact acatagccaa
     9601 gacgaatacg gtcgataaaa ttgccaatcg caccagctag aatcaaactc aatccaatca
     9661 tgaaccagcg attacctttt tcacgatttt taatcatcaa tgtgagaatc acaacgactg
     9721 caatgagtgt cactatagta aagaaccaca tcttcccttc tagcatactc catgcggctc
     9781 cagtgtttcg aatatgattc agtgacaaaa tacctggtat aagatttttc gtctctccaa
     9841 gcgctagatt atcagtgatc caccatttga ccaactgatc cactcctact agcaaagcgc
     9901 tgaatatcca ataaataatt aacaaaaaat caatcctttt ctatttgttt ataaatatca
     9961 cgaggtcgta cgatcccgag cagattacca tcgggattcc catctttagt gataagtacg
    10021 gcttccaaag tcccttgctc acgaaacatc gcttcaacct cgtaaatatg tgttttagct
    10081 ggaacaaaat gataatttgt gtactttccg ttttggatga tcaaatccaa cgctcgttta
    10141 tttgttaaat caaccggacc tttttggctt tcttttgcta gccaaaaccc gatattacgt
    10201 aaagtaatca atgctacaaa ttttccttta ttgtataacg gaaattgtga ataccttttt
    10261 tctgctacta tctttaacag ttcaagtaac ggaagatctt gttcaaaacc tgttacacgt
    10321 tttgcaaatc gaggcagcac ggtttcaggc tgaatcagtt cttgttcgat cagctggatt
    10381 cgcttgactg tccaatcatt tggctctgca atgacaaaat cgactgcgat ccgatcatgg
    10441 acgatcgcat tccttaactg agcaagctgt aaaagatcat cttcgtattt tctgatcatt
    10501 aattgttttc tttgagctag gcgtctgaca agttcggtaa atcccatatt tcgtgcattt
    10561 cccatctcgt ctctcatcca tttttcgatt cgattgaaac tgattaaaaa aagatcagag
    10621 tttgccataa ttttcctcct tgatcaaata tgcccggttt tttatataga aaaagaacgc
    10681 aatcaacaca ataactgcaa aaaaactgaa aaataaattt tgtaggaaaa cggcccccaa
    10741 cccagttgct agaaataaaa acggatagat ttgaagaatc aagtaattca ttgtgatcac
    10801 ataagcaggc tttgcttctt tcactgtata aacggggctt tttagccaat aaaaggtgac
    10861 ctgcatcgta aagggcactt ccagatcaag cgttatcttt tgttcatgac tgagaacact
    10921 gcgtttttca ttgttgattc ttagctgaat cgagctaccc ataccgtaaa aaccggtttt
    10981 tcttataaat gtcgttttca tcataccctc ctattatgct ttagtatagc tcaaaataca
    11041 ataaagaaaa agaatgatcc tagtgtaaac aaaattaatt ccctatcgtc taaaaaaaga
    11101 agctgtagtc aaaatacagc tccttcgatg gtagattata atcaagctta aaacaatccc
    11161 aacacttctc cgtcatttgt cacgtccata ttcaatgcag caggtttttt aggcaaacca
    11221 ggcatcgtca taacatctcc agtcaaggct actaaaaatc ctgcccctaa ttttgggatg
    11281 atttcgcgga tcgtgatggt gaaatcttcc ggtgcaccta gcagtgaagg atcatcagaa
    11341 aatgaatact gtgtttttgc catacaaatc ggcagtttgt cccatccatt cttcgtaaac
    11401 tcttctattt gtttttgcgc ttttttgctg aaaacaacac catttcctcc gtaaattttc
    11461 ttgacgattg tttctgtctt ttcttgtatc gtttcttcta ggcggtataa tggcttgtaa
    11521 ttagcttctt ttctatcaat caatcgtact acagcttttg ctaaatcaac gccgccttct
    11581 gctccatctg cccatacact cgctctcttg gcaagaatcc cctgattttc acacaagtgc
    11641 tccaacagtg tgagttcatt gtctgtatct gttacgaatt cattgattgc gacgatcaca
    11701 ggtagctgat attgttccat attacgtata tggcgcttta aatttgcaaa acctactttc
    11761 aaagcatcaa gattctcatt tttaagttca tcttttttca ttccgccatg cattttcaat
    11821 gcacggacag tggcgacaat caccacagca tccggcgctt ttttcaagtt aggtactttg
    11881 atatctaaaa atttctctcc gccaagatct gcaccaaatc ctgcctctgt gacaacgtaa
    11941 tctgctaatc tcaatgctgt cttggtagct aaaatactat tgcagccatg agcgatattg
    12001 gcaaacggtc ccccgtggac aaaagctggt gttccgtaaa ttgtttgtac aagattcggc
    12061 ttgattgcat ctttcagcag taatgccaga gcgccttctg ctttcaagtc tccagcagtc
    12121 acaggctgac ggtcaaatgt atagccgaca acgatccttg caagacgagc ctttaaatct
    12181 tcaaggtctg ccgcaagaca caagatagcc atgatttcgc tggcaactgt gatatcaaac
    12241 ccatcttctc ttggaacacc ttggattggc ccacccaagc caacaattac tttacgcagt
    12301 tcacgatcat tgagatcgac tacccgtttc caaatcactc ggcgggcatc gatgttcaat
    12361 tcatttcctt gatgaatatg attatctaaa agagcagata atgcattatt agctgttgtg
    12421 atagcatgca tgtcaccagt aaaatgcaag ttgatatctt ccataggcaa tacctgtgca
    12481 tatcctccgc ctgcagcacc acctttgatt cccatcacag gaccaagtga gggttctcgc
    12541 aatgcaataa cagatttttt attgatttta ttgagcgcgt ctcctaaccc tacagtgatc
    12601 gttgactttc cttcgccagc tggtgttgga ttgatggaag taactaagat caaatgcccc
    12661 tcagcgttat cctttgatcg attgattcca gaaaaattga ttttcgcctt atatttccca
    12721 tacagttcca aatcgtcttc cttaagatct aatttactag ccacttcaac aatcggttct
    12781 agctctgttt cttgtgaaat ttgcagatca ttcttcattt gattacccct tttcacgaac
    12841 tttattcaac aaaatatctt ctttgttaag atatgccaat tatacctttt tttattcaga
    12901 aaaaaaacat aaaattacag gtttttaatt tatttgacgt acttataaat actctttata
    12961 atgaaggcta tgatcagact ttagaaaggt cgtgagtgct ttgaccacag gaattgtaaa
    13021 atggtttgac aacaaaaaag gatacggctt tatcagttat gatgacacag aagaaatttt
    13081 tgtacatttt actgcgattg aagaagaggg attcaaaact ttggaagaaa atcaagtggt
    13141 cgaatttgag atcatcgaag ggaatcgagg aacacaagct gcccatgtca agaaaggcaa
    13201 cgttcagact accgatacta gtatgcatga atagataaac aaatagagtg agacgctaaa
    13261 cgggatgaac cgtacgtctc actcttttct tttcctgtaa aaattcgttt ttttaggttc
    13321 tgatttttaa aattagcttt ttcttttttt cgtataagga taaggaattg attctgattt
    13381 gggtccgttt ttttcaaaat gatcaaaaag gactacttga tctttttttc gcatcatgaa
    13441 aacagatggg gctttctcat cttgcaacat catttgcacc ccagtattag ttggcgtcaa
    13501 agatcgaatc tgtccgacag atatttctcg gtctttccaa aaagcagcat aacgtataat
    13561 cacagagttt gattttaaaa taaaagaacg ccgcaaacct agaaatagaa aaacaagaaa
    13621 aatacctaaa acaaaattgc ttttccaata aggacctgtg ttttctaaag aaagaatcag
    13681 actaaaaaac agaatgatca aggtcaatga ccaatagatg atcgtatgtg ccagttcagg
    13741 ctgccatcga atattttttt tcattctcta acctctcacc gattcagtaa tgatataatc
    13801 ttaccataac attagtttga acaaaaagga gaatctcttt ttatgttaaa agtatacatt
    13861 gatgcctcta caaaaggcaa ccctggtcct agtggaggtg gaatcttgat tgtctatcaa
    13921 ggtaaacaag aacagctatc tatcccgctt tctataggga caaaccatca agcagagttc
    13981 gaagtctttt taaaaacttt agaatttcta aagaaaaacg acttacagaa tgaaacgatt
    14041 ttctgttttt cagacagcaa aacactagtt tctacggtcg acaaaaacgc aacgaagaac
    14101 gagctcttcc ttccctacct tcaaagaatc caagaacttc tatccgaatt ttctttattg
    14161 atcctgcaat ggttgcccga aaacaaaaat aaaggagcag ataatcttgc acgtcaggct
    14221 ctccataaac aattaaaaga aaaaaagact ctttaaatta aaaactttaa ggagtctttt
    14281 tatagtttct catttaatat aaaagataat tgtgatcaaa cgtaatttcc aattggtaat
    14341 ctccgatttt ttcttctatt tgactacgta agattctttc tagttctgta tcaacaaact
    14401 ggaatttctc agggaccact acatcaaatt gcagcgtttt tccttgatga attactctaa
    14461 aatcatgaat attaagccct gtacctgttt caagaagaat ttgctttaac tgccgcaaga
    14521 tattatagta tttttcgtta tcaacaggca ttggatccaa gtgacagaca agttccacat
    14581 ccaaagactt tttaaagtct ttttctatca gatcaataat cttatgagcc acattcaaat
    14641 tcattgattc atctacttcg atgtggactg atgcaaagcg tttattgggt ccatagttat
    14701 gcaccaataa atcatgatag ccaagtatat catcgcaagc atttaaatgc tcttccattt
    14761 cttcaatctc tgctggcgtt gggcgacttc ctaatagttc gtatacaaaa tttcgaagca
    14821 tcgtcaatcc actataaagg atatataatg ctaaagcaaa acctatatat ccatcaatac
    14881 gccatccagt tacccattcg attgctgctg aaagcaatac agccagtgta gtaaagacat
    14941 cattataact atctgttgct gtagctctca atgtttcaga atcaatggat tgcgcaataa
    15001 cctgatacat tttgccttgt gcaaatttca aaaagatgga aatgattagg acaaaaaaga
    15061 cgatgggtgt taaagagaca ttttctggat ggatgatttt ttcagcagaa gactctaaaa
    15121 attgaaatcc tacataagta atgatgatcg aaacaaataa cccactgata tattcgaagc
    15181 gttcgtgtcc ataaggatgt tcctgatctg ctggctttgc agcaatttta aatccgatca
    15241 aagtcaatat ggatgaagca gtatctgata gactgttcac tgcatcagcc ataatggaga
    15301 cacttcctga aaaaagcccg atcattagct tagagacaaa caagatcaga ttcgtaacca
    15361 atcctaagat acccgctagt actccttttt ttgtccgctg ttgcggtatt tgtttctttt
    15421 tttcttcttg tttcttagcc aagtttttta ccccttctag tttgcttgtt gctcaggtaa
    15481 aaagatggag aggacgattg ttttcctctc ctctcttcct ttttctatct atcgtgcgtc
    15541 ctattactat taaaagatgg tcattattct ggtttcaacg gtgttgctgc aaataacgct
    15601 tttgctagta atggtgttat gattcccgca cctattgctt ctggtatccc attgattgct
    15661 gcaacccctg ctaaggtttt aaataaaaga gcagagtcta caccataggc atctgccgtt
    15721 gcacctgtat aaaataatcc catgaatcct aacactaaga ctgtattgac caatgaaccc
    15781 agtacccctg caaaggctga gctgatcgtt attgatttat ttttttgata aatccagtgg
    15841 aaaaccaagc ctgctactaa gccaacaagt actctgggaa ctaccgagat aatcggattc
    15901 gtgaacacta atgtatcaaa tggcgttgtt ggcatggcat aggcacgaac aattgtagcg
    15961 attccccaaa acaagccaat caccatgcca tcttttgggc ctaatactac tgcagcaata
    16021 attactgtga tatggataat tgttaaacta atgaatccta atggaataaa ccccagccaa
    16081 ggaaccatcg cttgtacgat gatgattgct aacaagatcg cacgtaagac tagtcgaaag
    16141 gtcttatttc tactattcat tttttcaccc ctttttattt atactaatcg taaaaatttc
    16201 tgtcactttc gtcaactaaa gaccatccga atccgaccat ttgattagat gcgccatcca
    16261 attgaaaaaa gtatgagggc aagacttgtt tgtcttcgcc cccataatca tgtttcgttc
    16321 aatgtttgaa cttttgtttt tctgtcaaac gtaaacgccc tattgttgcg caacccattc
    16381 tttcgcagct ttaagtgcat ctggaatacc agcaggattt ttcccgccag cttgtgccat
    16441 gtctggacgt ccaccaccgc caccgcctac tttcggtgcg attgctttaa tcaagtcgcc
    16501 agcttttagt ccagcttcat ttgattcttt cgtcattgcc gctagcagac tgacttttcc
    16561 ttcttgttcg tttgctaaaa caagaatatc tgaaatattt ttttgtttcc attggtcagc
    16621 taattgacgc agctgattca tatctttcac ttttacttgt gctgcaatga ttgtttttcc
    16681 attgatcgtt tctggatctt tgaatacatc accagcttgt tgattcgcta acttgctagc
    16741 taattgttca ttttcctttt gtagctctct tacttgctgt tgcaattgtt ccgcttttga
    16801 aacaacttct ttcaattgag gtgatttaac aattgttgct acttgtttta gttgtttttc
    16861 ttcttcttca agtagttcgt acgcttcttt acttgttaca gcctcgattc gtcgcacgcc
    16921 tgcaccaatt cctgattcag aaacgatttt gaagattccg atgttttctg tatttgctac
    16981 atgtgtgcct ccgcacagtt cgattgacca gccgccaata tttactactc gaacgtcatg
    17041 tccgtatttt tcaccaaaca aagccattgc acccatgttt ttggctgtat cgatatctgt
    17101 ttcaattgtt tcaactggga tagcttccca gattttttcg ttgacgattc tttccatttc
    17161 agctaattca tctgaagtga tttgaccaaa gtgggtaaag tcgaatcgta aatgtccagg
    17221 agcgactaaa gaaccagctt gatttgcatg ctcgccaagt acttctttca atgccttgtg
    17281 cagtagatgg gttgctgtat ggtttttgat gatccgattg cgcatcgatt gatcgatcaa
    17341 tagatgatag gtttgtcctt cactcattgg tgcttttact tctacggtgt gcataaattg
    17401 accattaggc gctttttgaa catctgtaac aactgctacg atttcgccta cttcattcat
    17461 gatccatcct tgatccgcta cttgcccacc catttctgca tagaaaggtg tttgatcaaa
    17521 gatcatttga gcttcacctt ctgcgacttc attcaaaaga gactcacctt taacgatgac
    17581 caataattga ctatctgatt cttgttctgt gtatccaatg aatttacttt ctactttgat
    17641 atctgtcaat aaagcagatt gtacccccat tgatttttca gtactgcggg cagagcgagc
    17701 acgttcgcgt tgtgcatcca tttctttttc aaaaccagca tgatcaactt tcagtccttc
    17761 atcttctgcc acctcttccg ttaattctac cgggaagcca tatgtgtcgt atagtttgaa
    17821 gatgtctttt cctgctagag tcgtcccgtt ttcgtttttt acttcttgga tcacttgatt
    17881 taagatttcc aaaccttcat tgattgtttc attgaaacgt tcttcttctg tacggataac
    17941 tttttcaata aattcttttt gttgaagtac ttcaggataa taacttacca tgatttcccc
    18001 aacaacaggg actaaacgat ataagaatgc ttgttcaata cctaattttt tcccatgcat
    18061 gacagcacga cgaagcaaac ggcgtaagac ataccctctc ccttcattcg atggcaatgc
    18121 accgtcgcca atagcaaatg aaagggcgcg aatatgatct gcgattactt tgtaagaaat
    18181 atctgttact gggttttcac catattttac ttgtttgctt agcttttcta cggcgtggat
    18241 aataggcata aacaagtctg tctcaaagtt cgttgacgca tcttgaacga ttgagaccat
    18301 gcgttccaat cccatccccg tatcaatgtt tttatgagga agtggttcaa atgtgttatc
    18361 aggcttatgg ttgaattctg aaaatactaa gttccagatt tctaaataac gttcattttc
    18421 tcctcctgga tagttttcag gatcgtcttc tgctaaatcg ttataagctt ctccacggtc
    18481 atagaaaatt tccgtatccg gaccacatgg gcctgcaccg atatcccaga agttgtcttc
    18541 tacatcaata atgtgatcta gtggcaggcc aacttcttca tgccagatgc gttttgcttc
    18601 tgtatctttt ggataaactg ttacataaag tttttctgga tcgaatgcca tccattttgg
    18661 atcagtcaaa aattcccatg cccagtggat ggcttcattt ttgaaatagt caccaatcga
    18721 aaaattaccc agcatctcaa acatcgtatg gtgccgtgct gttttaccga cattttcgat
    18781 atcatttgtt cgaatagatt tttgggcatt tgtgattctt ggattttctg gcacaactga
    18841 accatcaaaa tattttttca atgttgcaac accagagttg atccacaata aggttggatc
    18901 gttcacgggg atcaaagacg cactaggttc aatcgtatgt cctttagatt ggaagaaatc
    18961 aagaaacatt tgtcgaactt gtgcactact taattgtttc atgtcattac ctgctttcta
    19021 taaattaaat acaaaaaaac accatcacag ccaaggacga aaactcgcgg taccaccttg
    19081 attgcaatga tgtgtaatca ttacctctta aactcgataa cgctgagctg cgtcggtatt
    19141 tctaccaaaa tagaaaaagg gagcaatttg attagtgact attctttttt cagccaagaa
    19201 agaattttct gtgagtctta aaaaacaaat catatccttt tcgtacttaa ttagtatacg
    19261 cattctattg atcactgtca atatctcaat ttttattttc atttcctttt ggtcatactc
    19321 cacgattttt ggtacgataa gaataaagga atcaatacat ctctgctatt catgacaagg
    19381 aggaatatga tgaaaaaaat agttggagcg gatgaatgga tacgcgcagc tagctgcttt
    19441 ttgagatatc aagtttttgt attagaacaa gggattcctg cagaactaga attcgacgga
    19501 aaagatatcc cgcaacaaac ctattatcta tttcttgatc aaaaaagacc agtagctact
    19561 ttgcgttatg aaaagaaaac acctacttgc ttcaagccgg aacgattttg cgtagcaaaa
    19621 gaataccgag gaaaagggtt aggcggtcag ttgcttgaag ctgctgaaaa acgggcggtt
    19681 tcagaaggtt gtcagactag ttatgtagta gctgaaatga cggctgtaga tttttatgaa
    19741 aaacaagggt atcatacttg cacagaacct tttattgaag atgaagtact ttgtatcgga
    19801 atgaaaaaaa gtctagctta aaaacaagag attgt
//
LOCUS       DASJON010000032        19694 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJON010000032 DASJON010000000
VERSION     DASJON010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.032"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..210)
                     /locus_tag="U3989_000878"
     CDS             complement(<1..210)
                     /locus_tag="U3989_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714343.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="U3989_000879"
     CDS             complement(216..446)
                     /locus_tag="U3989_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714344.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="U3989_000880"
     CDS             1063..1725
                     /locus_tag="U3989_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714345.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="U3989_000881"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="U3989_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1714346.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="U3989_000882"
     CDS             5145..5459
                     /locus_tag="U3989_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1714347.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="U3989_000883"
     CDS             5472..5846
                     /locus_tag="U3989_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1714348.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="U3989_000884"
     CDS             5856..6200
                     /locus_tag="U3989_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN1714349.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="U3989_000885"
     CDS             6271..7623
                     /locus_tag="U3989_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1714350.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="U3989_000886"
     CDS             7683..8825
                     /locus_tag="U3989_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN1714351.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="U3989_000887"
     CDS             8850..9104
                     /locus_tag="U3989_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714352.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="U3989_000888"
     CDS             9360..10544
                     /locus_tag="U3989_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1714353.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="U3989_000889"
     CDS             10541..10678
                     /locus_tag="U3989_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1714354.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="U3989_000890"
     CDS             10683..10904
                     /locus_tag="U3989_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714355.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="U3989_000891"
     CDS             10917..11417
                     /locus_tag="U3989_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1714356.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="U3989_000892"
     CDS             11514..13361
                     /locus_tag="U3989_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN1714357.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="U3989_000893"
     CDS             13364..14260
                     /locus_tag="U3989_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1714358.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="U3989_000894"
     CDS             14310..14699
                     /locus_tag="U3989_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1714359.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16188
                     /locus_tag="U3989_000895"
                     /pseudo
     CDS             14686..16188
                     /locus_tag="U3989_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="U3989_000896"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="U3989_000896"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1714360.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18893..>19694
                     /locus_tag="U3989_000897"
     CDS             18893..>19694
                     /locus_tag="U3989_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1714361.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DASJON010000033        19328 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJON010000033 DASJON010000000
VERSION     DASJON010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.033"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(338..1291)
                     /locus_tag="U3989_000898"
     CDS             complement(338..1291)
                     /locus_tag="U3989_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1714362.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="U3989_000899"
     CDS             complement(1314..2069)
                     /locus_tag="U3989_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1714363.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="U3989_000900"
     CDS             complement(2066..3025)
                     /locus_tag="U3989_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1714364.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="U3989_000901"
     CDS             complement(3018..3968)
                     /locus_tag="U3989_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1714365.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="U3989_000902"
     CDS             4118..4570
                     /locus_tag="U3989_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN1714366.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4659..6926)
                     /gene="gshAB"
                     /locus_tag="U3989_000903"
     CDS             complement(4659..6926)
                     /gene="gshAB"
                     /locus_tag="U3989_000903"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN1714367.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7113..8111)
                     /gene="ftsY"
                     /locus_tag="U3989_000904"
     CDS             complement(7113..8111)
                     /gene="ftsY"
                     /locus_tag="U3989_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN1714368.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8122..8931)
                     /locus_tag="U3989_000905"
     CDS             complement(8122..8931)
                     /locus_tag="U3989_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1714369.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8940..12521)
                     /gene="smc"
                     /locus_tag="U3989_000906"
     CDS             complement(8940..12521)
                     /gene="smc"
                     /locus_tag="U3989_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN1714370.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12541..13227)
                     /locus_tag="U3989_000907"
     CDS             complement(12541..13227)
                     /locus_tag="U3989_000907"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN1714371.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13575..15356)
                     /locus_tag="U3989_000908"
     CDS             complement(13575..15356)
                     /locus_tag="U3989_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1714372.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15382..16296)
                     /locus_tag="U3989_000909"
     CDS             complement(15382..16296)
                     /locus_tag="U3989_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714373.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16323..17285)
                     /locus_tag="U3989_000910"
     CDS             complement(16323..17285)
                     /locus_tag="U3989_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714374.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17285..18229)
                     /locus_tag="U3989_000911"
     CDS             complement(17285..18229)
                     /locus_tag="U3989_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714375.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18230..19234)
                     /locus_tag="U3989_000912"
     CDS             complement(18230..19234)
                     /locus_tag="U3989_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714376.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5322 a         4187 c         3420 g         6399 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag ataatgaatt cacgttgaaa
      181 aaagatttct caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgtcgg ccagaaatga tgattagatc tggctgaagc tgattaattt
      961 tttctaagtc tggttctttt attccacctg ctgattctac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc tggattattt ggcacctcga
     1141 tttggtctcc attactatct gttatcgtca atgtagtttc agtttgtgtg ctgctttttt
     1201 ttgtgtctgc ctgactggtg ttgttgctat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcggcc ttcaatctca caaatccgta tgtccatttc gtataattca
     1381 tccaatacat ctttttttat catttcttgg gtcgttcctt gcgtaaataa ctgtccatct
     1441 ttcattgcaa caatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaaa ttttttaaaa tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aatgtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgcaca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgtcccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccagattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat tcccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctggaggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgtttc actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagctg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa aacgaaaaaa cacataaatt tttacatgca aaaaaagctt ttgatcagat
     4621 tgacatcaaa taggacaatc tgtcaaaagc tttttctttt aaaatacttc aggatataac
     4681 aatttcaata cattcatcgt caatcttctg ccttttcctg caaaaggata aacatgcata
     4741 tgcatggctg gattgaaatt tgcttcgatg atcccatatg cttttttatc agttgtagga
     4801 tcgatttctt tatctggtat aatcaaatcg ataccactaa tcttagctcc tagtgcttct
     4861 accgcagaaa cggcgatttt tttgtaagct tcagaaaatt catcagtcat atcgatcgaa
     4921 tcaccacctg tactgatatt tgagttctcc cttagataaa caatttgatt ggcttgggga
     4981 actgattcga tcgtcaaccc ttgttctttc aacatcaatt gttctaattc acctaattgg
     5041 atcaattcta aaggtgcacg atgattcgtt ccacgcaaag ggtcactatt tttttcttca
     5101 acaagttctt tcactgttcg gatactgtca ccaataacat tagcagggac tcgcaacatg
     5161 atggcttgga cttctccgtc gatcacaaag aaacgatatt ccgttcccgg cataaattct
     5221 tctactaaaa ccgaactgtc ttcacgaaaa gcaatggcta aacctgcttg ataatcttcc
     5281 agactagctc cttccttgaa aatcgtgata cctaacccgt aattggttga tttaggtttg
     5341 atcacaaatg cctgctcggc aaatctcgga taagctttta ccgcttcttc catggatgaa
     5401 aattctgcgc ctcctggtac ccggaaccca gcttctttta agactttttt cgttactgtt
     5461 ttgttttcca tgataagtgg gacgatatag ctgtccttac ttgtcatgtt tgcatttttc
     5521 acatattcta catggtcttg gaattgcaga cgcaaaaatt gatccgactc atctaatact
     5581 ttgacttgga cacctttttg gatggcatcg aacataaata tctgagtaga aagttccatt
     5641 tcgcgaaaac cggccaactg atagggacgt tcatggcttt ctttataata ttgatgtccc
     5701 aatgccacag caacttcttg ctgactcgat agctgtgctt cttgatagag tttcgctgct
     5761 aacgtttgtt caggatgatc catttgagtc aatgcttcct ctactaaata aagagaatcc
     5821 aaccaatcca attcaaccag catttgcttc attccttcaa gaatttctct accttcttga
     5881 tgaaattctg tttgatctga tggttgttct agtgctactt tgttatttcg agtcgtacct
     5941 tctgctaccc attggtcaca gtcttctttt tcttctgtcc ataacatgta catcaggaat
     6001 aattgtaaga accgaacttg ctctggattg atacccaatc ttgcataagg attcaaatcg
     6061 atattccgca attcaatata acgaacacct gttgttgcta aatctgcgac tttctttccg
     6121 cctcggaaac gaagcggtgt ataaaattct ttctcctccg aaagtttccc ttcttcgatc
     6181 attcgctcga tatctgctaa atactgtttc atagaagcat aggaaacttt cacatttgga
     6241 tgattcgtat agcccagcgc actgttgcga atactgcgaa caggttcttg cggatgtgat
     6301 tcatctaaaa agtagttctt ctcactcatt ggtgaagcac cgaataaata agtgatcatc
     6361 cagcgatagc gcataaagtt tctcgctgtt ttcaaataaa gttctgtttt gaattcggaa
     6421 aaatcttgaa attcttcctg atggctgaac aatgttctta gcaactcatc gccaaattca
     6481 aaattgaaat gaataccgct taccatctgt tttcgcttcc cgtattcttt tgctaaataa
     6541 cgtcgataca agacatcttc gaaatttttt aattttgcaa taatgatctc ttcgtctttt
     6601 tccggtaagg caggtggcat acttaatggc cagatcatct cttctttcgg tatagaacga
     6661 gcagtcacat cataaacagc agcgagatac tgaaataatt cgggaataga atcggtaaca
     6721 ggggtgatca tctctgtttg tgtttcgcta aaatctgttt ggatataggg gtgataggat
     6781 cgatcgccaa agattgctgg atgatcggtt tttgctagat ttcctgcaag atcaacccgc
     6841 tgtccttccc gttctatacc aaatcgagct tggtcgataa aagggcgcgc attgacatgc
     6901 aataataatt gcttaaaatt catcattaag tgacctccaa ttcatatgaa tctaattata
     6961 atcaaaaagt ctaagaaaaa gaacgaaatt gtttgagaac cgcttacaaa taagtataaa
     7021 aatgaaggca tcaaaaacac agctatgtta aaaacaatca ttcaaacaag gagctgcaac
     7081 ttttgtcaca gctcctgctt tttagacatg ggttattctt cttttagtaa tcctttgaac
     7141 aatccaacga caaaatcatt tggatcaaac ggctccaagt catcgattcc ttcaccaaga
     7201 ccgaccaatt tcactggtaa atgcaattca ttacggatag caagtacgat accgcccttt
     7261 gctgtaccat ctaattttgt caaaacaaga ccggtcacat ctgttgtttc tttgaattgt
     7321 tttgcttgaa ccatcgcgtt ttgaccagtg gttgcatcta atacaagaag tgtttcttgg
     7381 ggtgcttctg gtaattcccg ctggattact cgttttattt tttctaattc gttcatcaag
     7441 ttcactttat tttgcagtcg gcctgcagta tcaactagta aaacatctgc ctgctctgtc
     7501 tttgctcttt ccaaagcatc gaatacgaca gctgctggat cgcctcctgc attgccacga
     7561 acaacttcaa caccagcgcg ttcgccccag acaactaact ggtcgattgc tccagcgcgg
     7621 aaggtatctg ctgctgccat caatactttt ttaccttcgt ttttgaactg atgggcaagt
     7681 ttaccaatac ttgttgtttt ccctacacca ttaacaccga caaacaaaat aactgttagt
     7741 ccgtcttctt ggatatttag ttcatttact tcattgattc cttccgcttc atagagatcc
     7801 acaagtttct cgatgatcgc attttggact tctgccgatt ttttcgcatt tctgagtttc
     7861 acttcttgac gtaatgcttc ggtgattcgt atcgctgttt caaaacctac atctgcaccg
     7921 ataagtgttt cttctaattc ttcaaaaaag tcttcatcga ctgttcgaaa attggcaaac
     7981 aactcattca tccgttcacc aaatgtttta cgcgtctttt ctaaaccctt gtcgtacttc
     8041 tcctgtactt caacttcttc tgttttctct tcgccagtaa aagcacgctt gattttatca
     8101 aaaaatccca tttgactccc cctactctaa tacaaatttt tggatcactt tggctacgcc
     8161 attttcttca ttgctggctg tcacgaaatc tgctgcttct ttgacgaaag gcacagcatt
     8221 ctccatcgct acgcctagtc cagcatattg gatcatcggt aaatcatttt cctcatcacc
     8281 gatcgccatg atttcttctt gtttcaaacc aagatctttt gccaataaag aaataccata
     8341 agcttttgta acaccttttg gcataaattc caaaagcata cttcttgttt tgatgatctc
     8401 atacatttca tggaattctg ctggtatttc tttgatttta ggatctaatt cttcttggtc
     8461 ataagcgatt actgccttat taagcacaaa ttcatctgta atatcgctta tatccgctgg
     8521 ttggaattgc agcagcttat tcaacacatt gtacaaggat tgcttccttg gggcactagg
     8581 cagctgtaaa accacattgt cagacaaaac atctagtggc agatccaatt gctgagccag
     8641 tttgtataag cgttggatat ctgatacttc catcactttc ttttccatga tttcaccagt
     8701 atcatttttt tgtaccaaac cgccgttgaa agtgatgctg tagtcacctt catctaccag
     8761 tcctaattct tgcagataat gcgccatcgc agctaagggg cgtcctgtac atagaacgat
     8821 cttgacccct tgctgttttg cttgtgctaa tgcttgttta ttctcatctg aaatcagctt
     8881 ttgttcattt aataatgtac cgtccaaatc gattgctact aatttgatca ttttcgttcc
     8941 tacactttcg tttcgttgat tttcgcctct tcattgatat cttccatccg tacagaaacg
     9001 atttttgata caccggattc ctgcatcgtc actccgtaca gtacatctgc tgcgaccatc
     9061 gttcctttgc ggtgtgtgac tacgataaat tgtgtgtcat cttggaaatc gctcaagtaa
     9121 cgaccaaaac gtttcacgtt ggcttcatct aacgctgctt ctacctcatc taagatacaa
     9181 aacggaacag gacagacacg gataatggaa aagagcaacg cgatggctgt aagtgctcgt
     9241 tctcctccag aaagcaaact tagactttgc agttttttgc caggaggttg tacttcaatc
     9301 tcaatacctg ttttcagcaa atcagttggt tccgtcagta ctaattcagc tttaccgccg
     9361 ccaaacatat ttggaaagac aattttgaat tcttgacgga tagcttcaaa tacttctttg
     9421 aatcttgtcc gcacttcatc atccatctca tccatcgttt caaacaattg gtttttcgct
     9481 gctaacagat catctcgctg tgttgctaag aatgtatgtc tttcactgac ttgttcgtat
     9541 tgttcgattg aatttaagtt tactggtccc agtttttcaa tctgttcttt taaggatgaa
     9601 acctttgttc tactgtcttc gatatcggtt gtttcttgat agtcttggga agctttctca
     9661 aatgtcagct gatattctga ctgcagatag gtcaaatgat tatctaaaat catttcagct
     9721 cggtcttttt ggacttctaa tttggattgt tccgacagtc gtgctttttg ctcacgatta
     9781 cgctctgcca agactgcttc aagcttatcg atctcctttt gccttctatc tcgctgctct
     9841 ttggcttttg ccaactcggc ttttaatgtt tctcgctgag cagataattc gttaatttgt
     9901 ttttccaaac tttcttctgt gacttcatga tcagaaaagt ctgctgtcaa tgtcgccaac
     9961 tgcttttcta tcgcttcttg acgttcggtt gcctctgctt tttggactcg ggcaccgcgg
    10021 agctggatct gcagatgatt aaactgctct ttcaagacag caagatctgc ttgttcttgt
    10081 gctttttgac tttgtacttg tgctcttctg gcttccatct ggtcgctttc ttgacttaac
    10141 gatttgattt cttcgtcgat tttttgtcgt tgcgattcaa gatctgtttg tttgtctttc
    10201 aattcgttcg tttgcttttc gtaatcttcg ataaactgct gcagttcacg tgtctcaaaa
    10261 gttgagattt gcttttcttt ttcaaaacgt tcaagttcat ttatgatgtt ttgtagctga
    10321 tttgtcgctt cttgttcttc aaaacgtaat tgttcgcctc gtgtacgcaa aacttcttgt
    10381 tcttcggcta aacgagctgt ttcttgttgc agctcctgaa cttttttttc ttgtgcttgg
    10441 agttgtttat ctgcttcttc aaattcacta gtcaactgtt tcaattcttg attttgtaca
    10501 aacaaactgc cttggtttcc tcgtttattc gctccacctg tcatggatcc tccggcattc
    10561 atcacatcac cttctaagga aaccactcga tattgatagc ggatcgtttg cgcgattgca
    10621 ttggcactag ttaagtcttt ggctaaaagg atcgttccta gcaggttttg aaccacagtt
    10681 tggatttgat cgggataact gacttgttca ctggcaatcc ctataaaccc ttctacagct
    10741 gctgcttgtg tcaatatatg agccggtaat tgtcttggtt tgattgtcgt cagtggaagg
    10801 aatgttgctc ttcctcctct ttgctgtttc aaatacgtga tggcttgccg agcatctttt
    10861 tcattttcaa cgatgacatg ctgggctgcc ccacctaaag ctgcttcgat agctaacgtg
    10921 aaatctgcag gaacatcgat cagttcagcc actgctccga caatacccga aagctgctgt
    10981 ttatgctgca agaccaatcg aaccccttgg taaaaaccaa aatagttttc ttggatttct
    11041 tgaaggcttt tcttacgagc acgtagctgc tggacttgat tcattaattg atacatttta
    11101 ggctgttctt ttgctaatgc ttcttggaac tttttcccgt tttgttggat catttctaac
    11161 ttctcttttg tttcagttaa agatgatttt agctgcgcct gcttttgtgt caattcttct
    11221 ttttgtaaga tcaatcggtc tactgatgct tctacttccg attgcttggc taacgtctgc
    11281 ttgctttttg ctgtttcttg tatatattga cgttctagat acttcagttc gtttcctaca
    11341 gctgcttgtt cttgcatcaa atcaacatat tgatctctta attctgccaa taattctttc
    11401 gttgattttt ggtatttttc taattcttgc tgtgttttta tgagatttgc ttcagctttt
    11461 tggatctctg tttctttttc tgctgctgct ttcatcaagc tttcttgaag cttttcaaaa
    11521 tgtttaacct ttttttgtgc ttctgctaat gaagtttgat attcttgcga gcttttttgt
    11581 gtatgctttg tccgctcctg caaaacatct ttttgtcctt ctgtctgctt cagtttttcc
    11641 gaaagatcta ataatacttg ctgatttttt tcgatcagac gatcggcttg cgcattttct
    11701 ttacgctgct tcgccaaaat ggattcttgt tcttggatcg actcagatag tttgcctaat
    11761 tctagattga atttcgcaag ctgtgcttgt ttgttgtccc aatctttttt tgctgtcttg
    11821 atttcagcaa ccatcaaaga gacatcggtt tgagtcagtg tttcttttag ccgtaaaaac
    11881 tcctttgctg cctcactctg ggcggccaaa ggagttaact gttcttccaa ttcgtggatg
    11941 atgtcttgga cgcggctgag gttgtcttct gtttcaaaaa gcttctgttc tgctttcttt
    12001 ttccgctgct tgtatttcaa gacaccagct gcttcttcaa agatcccacg acggtcttcc
    12061 ggtttactac tgaaaatcgc ttctactttt ccttgagaaa tgattgagaa cgattctttt
    12121 cctaatccag aatctaagaa caattcttgg atatctttta atctgcagct ttgtttattg
    12181 atgaaaaact cgctttcccc agtccgtcga taacggcggg ttacactgat ctcattgtac
    12241 tccaatggca gataatgatc actattgtct aaaatcactg tgacttctgc aatattcagc
    12301 tgcttacggg tatcggaacc ggcaaagatg atatccggca tttttccgcc tcgcagactt
    12361 ttagcagatt gttctcctaa tacccagcgg atagcctctg taatattgct ttttccgctg
    12421 ccatttggtc caaccactgc tgtgacgcta ttttcaaaat caatgactgt cttgtctgca
    12481 aatgatttaa aaccggctat ttcaattcgt ttcaaataca aagctttccc tcacttactt
    12541 ttacagtgct gccaatgcat tttccgccgc agcttgttct gctaattttt tcgatttccc
    12601 ttgtccagtg ccaatcaatt gatcatctac ataaacttcg atccaaaata ctcgttcatg
    12661 agctggacct tcttcattga tcaatcggta ttcgattgat acatcacctg atttttgcaa
    12721 gacttcttgt agttttgttt tatgatccat ctcatgtgaa aaagcacctg ctttgacttt
    12781 aggaaaaatc actttttcga tgaatgcatg ggtcgtgtcg aatccctggt caagatataa
    12841 cgcgcctaaa aaagcttcga ataggtcgca aagcaatgct ggtcgcgtac gtcctcctga
    12901 attctcttct cccttgccca acaaaacata ttgatcaaag tgacattctt tggcaaattt
    12961 cgacaagctg tcttcgcgaa cgatcgcagc tcgtgttttt gtcaattttc cttctggcaa
    13021 atgaggatat aaacggaata aatactgaga aaccatcagt tctaaaacgg catctcctaa
    13081 aaattcaagt cgctcattat ccgataattg cagattgcga tgctcattca cataggatga
    13141 atgggtaaaa gcttgttcaa gcaaatttag atcgtgaaaa acgatgttat agttttcttt
    13201 tagttctttt gtcagctggt tgtccatttt ccacattcct tttatggata ttttttcgat
    13261 acttccttat tatactggtt ttttgtcaaa tttgaaatgg ttcggtcaaa aggtaagtaa
    13321 agctttaata atcctagtga ttgacgagaa gttgtgaaaa attcgtttag tttcgagtat
    13381 gaagaaaaaa ttttgtgaaa atggctttcc atatttttgc ataaatttga cttaataccg
    13441 agaaactgga ctttgaacac cgtttattgg agaagtcatg aaatcagcgc cttgacctga
    13501 atattagaga aataaacaga aaaatatcaa aaaaccgaaa aaggacatcg ccctttttcg
    13561 gtcattttta gtgattatga acgtgaatct gctgtaacag aaacatttgc ccatgggttt
    13621 ggtacatcat aagaccaatc ccagtctttg acacggtcgt tgactggcaa tacttcatta
    13681 cggaacaacg ttggaatcgc aaatgcttct tcaaatgcat attgttgcca tttttgatat
    13741 gcttcttttt ggtactcttc atcaaatgct ttgcttgaac tgatatcgtt gatcaatttt
    13801 gtattttctt ctgattcata acgagtgtag ttgaacgcag catttggtcc ccatagtcca
    13861 cttggatttg gatcagttcc tgtattccaa gcagcttggt aaacatcaat ttcaggatca
    13921 tcgttttcta atttatcata gaaagaattg aattcgatca gacgacctgt tgtgtattgg
    13981 acatccaaac caatttcttt ccattgttgg atatagtaat ctgccaatgg ttgcgctgtt
    14041 tccccaccgg acatcgaagc aaagttgatt gttagtttat ttccatcttt gtcttctctt
    14101 aaaccatcgt catccacatc tttgtagcca gcatcatcta atagtttttt cgctttgtct
    14161 agatcttgtg tgaatccttt catctcatca tcatggtatg actggaatac tggaggaatc
    14221 aatgaagtcg cattgcttct caaacctgca tagaaacgtt cacctactgc attattatct
    14281 aatgcatagc ccattgcttg acgtaatgat ttatcagcca tttttgaatc tggattgtat
    14341 tccacactct ttgtatctgc attccattta cctaacttga atcctagata agtataagaa
    14401 gtttgttcac ggcctaagtt tgtatagcca gcaatttctt tataggtatc gtaagtatct
    14461 gttggcattt ggaaaatcat atcatataat ttagagttca atgcttcagt tgcacttgcc
    14521 atcggtacag atttcatcgt gatctttttc aatttaggcg taccattcca gtagtgctcg
    14581 tttggcaagt attctactga ttctcctgca acgatattac tcatgtaata agggccaaaa
    14641 gtcactggat ttttacgtac ttgatcgctt gattccatat ctttgattgc tacaccttct
    14701 aacgtatgtt ttggtgctgc atatgaccag ataccaccac ccgattgaag catgcttggg
    14761 tttacttctt tatattcgat ttcaactgtt ttgtcgtcta tttttttgat tccggagatt
    14821 gtgtcggcac ttccatcatg atattcttcc atacctacga tgttacggaa agtgtcgtca
    14881 taacggatac cagtgtaatc tttattccca atgatttcgt aagggaaaat cacatcgtcc
    14941 gccgttacat cttcaccatc tgaccatttg acgttgtctt ttactttgat tgtcgctttt
    15001 ttcgtatctt catctagatc aagcgttgca gctccgtcgt ctgtgattct gaaatcgtca
    15061 tcgtttgcaa atagcggttc atgagaagga gccataaaag ctgcatcata tgaatcttgg
    15121 taaaactccc actggaacaa tcctttaaac tgactgtccg ttgcgaccgc agcttctaat
    15181 tcgccgtctt cgattgcatc atcattattt tttgttgcaa ttggaaaagt taattcctct
    15241 ttgttattgc cgttgcctcc agagttgttt ccagaggtat ctgtacctcc gcccccgcat
    15301 gctgcaagaa cgacagcaga catcagcgct gcaaatccaa aaatcgtttt cttcattcct
    15361 atttccccct tcgcttctat actagcctaa ccgttggcgc gcatctgcag aacgcttgaa
    15421 tgcttgtcca acataattga tactcaacat caaaactaaa ataaggattg atgcgggtaa
    15481 ccagatccat tgccgattca ctaagacttc tccgttactt gcataaccga tcaatgtacc
    15541 taaactaggt acatttgccg gcaagccgaa acctaagaaa gtcaaagttg tctcgatacc
    15601 gatgtttgcc gcaaagttca tcgtcaaatt agtaatgatt aatgaactta gattcggcat
    15661 gatttcgcgg aacatgattt taaaatcatt ggttcccatt gtttttgagg cactgacata
    15721 atctcttctt gcttcagata atgtcttgct tcggaataat cgggctttag ctacccaata
    15781 aaatgcactc atgatgaata taaaggacca gacattgtat ctggaaataa tcgatacgaa
    15841 gacgataatg atcagcatga tcggtagaat catgataaaa tcaacgatcc gcatcaagat
    15901 gttgtcaatg atccctccgt agtagccaga gatgatccct aatccaaccc cgatgatcga
    15961 agtaaggatc gtgatcgcaa atccgatcgt aacagagttt ctagcaccta tgatcaattg
    16021 tcctaataca tcacgtccgc cttcatctgc tcccagaatg aacttttgtc cttgtgtcga
    16081 aactgttcct ggtgcagcgt atttgtcgaa gatactaaca tacatcactt tgtcttgatc
    16141 ggtcagcatc gcaccgataa agatcactaa taataaaacc actagcaaac ctaatgaaaa
    16201 aatagcaatt ttatcttttt taaattctcg agcaatcatt cgtatcccca taggaggaat
    16261 actttcttga actgctgtat tgtttttttc actcatcgat ccattctcct ttctgattgg
    16321 ttttattgaa tccgaatacg cgggtcaacg atactcatga tgatatctga aagtaatgtc
    16381 cccactagcg tcgcgattcc taaaatcaat actaaggatg tgatcacggt atagtcccgt
    16441 tggccgatgg aatcaataaa tagttttccg actcccggat aaccaaaaat cttttcgata
    16501 actacagaac cagcaatcaa cgaagtgatt tcatacccca tttgagaagc catcggcaac
    16561 gctgcatttc ggaaaatatg tcgactgaat actttattcg tcggtacccc tttggaacgg
    16621 gctgttctga caaaatcgag cgatttcgaa tcgattactt cacttcgcag atattggatt
    16681 gtgacggcag tcgccaaaaa tgcttgggta attgatggta acagaatata tttcaaacgg
    16741 tctatccatt gagtgataaa tccaccttgc aaacctagtg tttgtgatcc acttgttggg
    16801 aaccagccta aacgataacc aaaaacaaat agcatgatca agccgaagat aaataaagga
    16861 acagcaaaac taaagaagtt gtagattacg acaatcttat ccaagattga attttgatac
    16921 cggccagcaa gcatccccag aggcacagca atcaaataag taaggacaac tgtcaagatc
    16981 gatagatata aagtgttgat gatccgttcg ccgatcaagt cagcaacaga gcgtttgtat
    17041 aagaaacttt gaccaaaatc gccttgcaaa gcatttccga tccaacggaa gtactggaca
    17101 taccatgggt cattcaagcc agctgcttgt cgcatctgtt cgatcactgc cggatcctga
    17161 tttggattga tcaaaccggt aaatggatcc cccggcatcg cttgggctaa taagaagatc
    17221 agaacactta aaataataac ttgcgggatc atcaataaaa cacgtcgcag aatcgttttc
    17281 cacattagct tgcacctcca tctttcaacg ccactttgtg tgtagttgtg atcgtacgca
    17341 aatcgtagac tcgaccagtc gcatcatagt attctttttg gttttggata tactcttgtt
    17401 ctactcttcg tcgattttct ttgtggcttt ccctattttc tacatcgatt tgcggaatag
    17461 ctgaaagcag acgtttcgta tagatatgtc gcgggtcatt ataaatgtct tctcttgtac
    17521 cgatttctac aaatcgacct ttgtacataa ttgcgatatt gtcgcacatg tgttttacta
    17581 cacctaaatc atgtgaaatg aataaatagc tgaggccgta ttcttgctgg attcgtttca
    17641 tgaaattcaa tacttgcgct tgaacagata gatccagagc agatacaggt tcatcggcta
    17701 cgatcagttt tggattggta gcaacagcac gggctactcc tagacgttga cgttgtccgc
    17761 ctgaaaattc atgaggatat ttatatagcg catcttccgg catcccaacg atatctagca
    17821 gtccttttac ctttttcttt tcttcttgat cgcttaaccg ttcaaaattt cggatgggtt
    17881 cagcaatgat atccaacact cgctttttag gattcaaact ggacattgaa tcttggaaga
    17941 tcatctgcac atccttgtta taacccattt ttttgcggtt gcttcgcttc gttacatctt
    18001 ttccttcata catgatttgt ccggctgtta ccttttctaa tcccacaacg gcttttcccg
    18061 tagtagattt tccagagcca gactctccca ctaaaccata tgtctttcct ttttcgataa
    18121 taaaatccac accatctaca gcatatacat gatccgttac tcgattgaag aatccactgc
    18181 gaatagggta atgaactttc aggtctttga ttgtgattaa ttctgtcatc tacacctctc
    18241 ctttttcgtc tctaaagtgg aaatgcttat agcaggagca acgtaccaag tggttcggtg
    18301 cgacttcatg aaggataggc tcttcttcat gatcgcttgc tggtatccat ggaatacgtg
    18361 gtgcaaaccg acaaccttta cgaggcatgt ttttcagaga aggaacgaca ccttcgatca
    18421 catggagttc tgcatcatga tttccttctt gtggtataga gttcagtaaa gagcgtgtat
    18481 aaggatgctt aggatgatca aacagttctt ttacacctgc tacttcaacg aactgtccgc
    18541 catacatcac tgccactcga tcagccattt ctgctacgac tcctaagtca tgagtgatca
    18601 agatgatccc tgtttttgtt tcttcctgaa ggtctttcag taaatcaaga atctgtgctt
    18661 ggatggttac atctagggct gtcgtcggtt catctgcaat gacgattgga ggtttgcagg
    18721 cgattgcgat tgcgatgatg acacgttgtc tcataccgcc tgatagttca tggggatatt
    18781 ggcgtccaac tctcgcagga ttaggaatcc ctacttgttc tagcaattct agtgcacgag
    18841 cttgacgctg ttctgcattc atctttgtgt gataggaaag tccttctttg atctggtctt
    18901 cgattctcat gagaggattt aatgcagaca acggatcttg gaaaatcatt ccgatatcat
    18961 ttccgcggat tttattatag agcgtttcgt tcaggtcgat taaattcaga tcgttatata
    19021 aaatctcacc tgtaattttt gtgttattag gatcaagtaa tcccataatc gtagtagcca
    19081 aggtactttt cccgcaccca gattccccaa cgattgctaa aatctcgttt ttgtctaaag
    19141 taaatgaaac gtcatctact gcatcgtaat agtcatcttt gagtcggaat cctgtatgta
    19201 ggttctgaac ttctaataat tggttatcgg acaatcgaat acccctctct gcttcctaaa
    19261 tggaagtttt tcttaataaa aaaggacatt acacagaata aatcgtttta taacaaaggt
    19321 tattctta
//
LOCUS       DASJON010000034        19261 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJON010000034 DASJON010000000
VERSION     DASJON010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.034"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..193
                     /locus_tag="U3989_000913"
     CDS             <1..193
                     /locus_tag="U3989_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1714377.1"
                     /translation="INFSKYLKKVRISKAKLFLTETRLTLTEICEKVGYTELGYFSRV
                     FKEETGMTPSQYRKGTFVK"
     gene            190..1926
                     /locus_tag="U3989_000914"
     CDS             190..1926
                     /locus_tag="U3989_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1714378.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(1989..2825)
                     /gene="pheA"
                     /locus_tag="U3989_000915"
     CDS             complement(1989..2825)
                     /gene="pheA"
                     /locus_tag="U3989_000915"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN1714379.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(2822..3331)
                     /locus_tag="U3989_000916"
     CDS             complement(2822..3331)
                     /locus_tag="U3989_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN1714380.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(3324..4619)
                     /gene="aroA"
                     /locus_tag="U3989_000917"
     CDS             complement(3324..4619)
                     /gene="aroA"
                     /locus_tag="U3989_000917"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1714381.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(4632..5723)
                     /locus_tag="U3989_000918"
     CDS             complement(4632..5723)
                     /locus_tag="U3989_000918"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN1714382.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(5740..6906)
                     /gene="aroC"
                     /locus_tag="U3989_000919"
     CDS             complement(5740..6906)
                     /gene="aroC"
                     /locus_tag="U3989_000919"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN1714383.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(6908..7972)
                     /gene="aroB"
                     /locus_tag="U3989_000920"
     CDS             complement(6908..7972)
                     /gene="aroB"
                     /locus_tag="U3989_000920"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN1714384.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(8005..9027)
                     /gene="aroF"
                     /locus_tag="U3989_000921"
     CDS             complement(8005..9027)
                     /gene="aroF"
                     /locus_tag="U3989_000921"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN1714385.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(9686..10660)
                     /locus_tag="U3989_000922"
     CDS             complement(9686..10660)
                     /locus_tag="U3989_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN1714386.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(10641..12524)
                     /locus_tag="U3989_000923"
     CDS             complement(10641..12524)
                     /locus_tag="U3989_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN1714387.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(12542..14767)
                     /locus_tag="U3989_000924"
     CDS             complement(12542..14767)
                     /locus_tag="U3989_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1714388.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            14882..15916
                     /locus_tag="U3989_000925"
     CDS             14882..15916
                     /locus_tag="U3989_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1714389.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(15983..16996)
                     /locus_tag="U3989_000926"
     CDS             complement(15983..16996)
                     /locus_tag="U3989_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1714390.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17074..17262)
                     /locus_tag="U3989_000927"
     CDS             complement(17074..17262)
                     /locus_tag="U3989_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN1714391.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(17702..17830)
                     /locus_tag="U3989_000928"
     CDS             complement(17702..17830)
                     /locus_tag="U3989_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1714392.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(17833..19206)
                     /gene="rlmD"
                     /locus_tag="U3989_000929"
     CDS             complement(17833..19206)
                     /gene="rlmD"
                     /locus_tag="U3989_000929"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1714393.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
BASE COUNT         5309 a         4169 c         3519 g         6264 t
ORIGIN      
        1 tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc tctttttgac
       61 ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg aactgggtta
      121 tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc gtaaaggaac
      181 ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact tctgttcatt
      241 ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta tcatcgatca
      301 agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa aaagattcaa
      361 tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt tgattctaat
      421 ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca gcttcagcaa
      481 ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat gatcgattac
      541 gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat gctcactgcc
      601 gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga acgtgctgct
      661 gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa agcacccagc
      721 gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata tgctgtttta
      781 tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc tgagatatcg
      841 aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga aaatacaatt
      901 gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat ttcaaatgga
      961 cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag tcaagtcgat
     1021 cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta taatatcgtg
     1081 acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac aaaatcgatc
     1141 taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc tactgttcct
     1201 gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt gctaagtaat
     1261 gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc gcttgaaatc
     1321 caaactatgc aagcacagat acagccccat tttatttata atacgctgac tagcttgaaa
     1381 tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag tttcattact
     1441 ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga agaaatcaaa
     1501 agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat ctcggctaaa
     1561 ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct tcaaccaatt
     1621 atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat cacggtgtac
     1681 ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga tggcttcgat
     1741 actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag ccatgtagat
     1801 gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc agtgattggg
     1861 acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc agaatatcaa
     1921 aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc aatcagtatt
     1981 cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc caatatttga
     2041 ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt cattggacgg
     2101 tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa tggtcgggat
     2161 tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt atgaagcatc
     2221 cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc tgactgctgg
     2281 ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc attatcttgg
     2341 atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga agcaatcgct
     2401 gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt tgattctgtt
     2461 gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg tgctagtgct
     2521 tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag ctgctgcttg
     2581 ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag aagatcgatt
     2641 gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag atcgacttgt
     2701 ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa ttgttctgta
     2761 ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag atagctgacg
     2821 ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt gtttgattgg
     2881 tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt gccaataatg
     2941 cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt tcatcttgtt
     3001 ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc ggctgtgttt
     3061 ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg ataattttgt
     3121 cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt ttttcaccgt
     3181 aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt gtatctaaat
     3241 ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt tttccagcgc
     3301 ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac aagttctgct
     3361 aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc tagttcgcaa
     3421 ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg atcgcctctg
     3481 ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc gtctgctgtc
     3541 gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat tctgttggtt
     3601 tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc ttgagtagcc
     3661 actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc accaatcgtt
     3721 gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg ttcattgtga
     3781 ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc agttcttgtt
     3841 ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag tcctgctgct
     3901 agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc agttaatttt
     3961 tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc aaattgacga
     4021 atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga tgtgccttct
     4081 gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac aggcatattg
     4141 tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata ctggtcattt
     4201 tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg ccccattgga
     4261 cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc agccaaaata
     4321 cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc ggttggttgg
     4381 acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc ttcgatctcg
     4441 acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg caaaaaattt
     4501 tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg ggaaattgat
     4561 ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa taattgcatt
     4621 tatccagcct cctatttgat ccagcaatga taactggtat tagacataat gattcttttt
     4681 gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa aatatcttcc
     4741 ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat cagtccagtt
     4801 acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa aaaggcagga
     4861 atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg attgaaaaaa
     4921 gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt catttcttgt
     4981 tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat atctgtccac
     5041 atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc tgccagttgt
     5101 ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc agctgccaga
     5161 atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc tgtaagagtt
     5221 acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc ttttttcgtc
     5281 ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc tgctgcctgt
     5341 atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag atttttctgc
     5401 tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat gacgtgatca
     5461 gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac gggaacggct
     5521 aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac gtaatcaacg
     5581 atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc aactactatc
     5641 agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag tccaagcccc
     5701 acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt tcaagtagtc
     5761 acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga attttgacaa
     5821 tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag ctgcaggaac
     5881 tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat cgatatcaac
     5941 actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa tgacaagtgg
     6001 ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc ggaaatagcc
     6061 tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg aaccagaagt
     6121 gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag cttgtgcgat
     6181 tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg gaacgcctcc
     6241 aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct gatcgatcaa
     6301 atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg cttcagaaat
     6361 ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc caccaagaca
     6421 aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg caattgcgcc
     6481 gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat tccgaagatc
     6541 tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg gacgtgcaac
     6601 tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag ttgtccaatt
     6661 tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt tgccgtgacg
     6721 gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc ctctgccgta
     6781 accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag gtaatcctgc
     6841 tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg cagtcatata
     6901 acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca ggaacgattt
     6961 ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt gctttcttat
     7021 catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta atcggcagat
     7081 ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac aagccgattt
     7141 tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca tgactgacta
     7201 ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa tttaaaataa
     7261 gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt tccaacgtag
     7321 cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc tcgttcagct
     7381 gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg attccttctc
     7441 gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca ccagctggct
     7501 ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc ttcccgccaa
     7561 tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg atcccacgca
     7621 tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca agtgcaagta
     7681 tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca gctgcttgtt
     7741 ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca aaattagctt
     7801 gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta tcagtgatca
     7861 cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt tctagtgcgc
     7921 ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc attcgtttca
     7981 cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca ttgcttcttt
     8041 catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg attggggacc
     8101 gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat ctgctccgct
     8161 tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac catgacttgg
     8221 atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac ttaaatcaaa
     8281 cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa tgacaggaga
     8341 tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg taccggaaat
     8401 tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca gtttaaaatt
     8461 ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca tatcgatatg
     8521 cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg cttgacggat
     8581 gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac gtggtttgaa
     8641 ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac attctctgat
     8701 ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc cgtcaccaat
     8761 ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag aagtcaattt
     8821 gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa atgctccttc
     8881 ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc cttgatcgat
     8941 tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt gttcttccgt
     9001 tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt tacttatcta
     9061 tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta ctaaatgacc
     9121 gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga taatcaacaa
     9181 aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga gccttaaaaa
     9241 gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc tgtaagtcgt
     9301 gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc acgaagtcat
     9361 gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta agttttctag
     9421 atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag acataaaaaa
     9481 atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta actttgtttt
     9541 tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag tatctgagaa
     9601 cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg taaactccgt
     9661 ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca tggtcctctt
     9721 cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata cccccgactc
     9781 ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt tcttctatat
     9841 gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca aatggcgtat
     9901 cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta tccaacgttt
     9961 gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct tggggaacaa
    10021 ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca ccagccatcc
    10081 gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct ttcgttggca
    10141 taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca taataatgca
    10201 tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact tgggtttttg
    10261 gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat tcattacttg
    10321 aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc accagtactt
    10381 ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat ttaaatccaa
    10441 atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt gtttcccggt
    10501 atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta taagaaaaaa
    10561 tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg attgcaactt
    10621 tttccgtatc catatgactt ttatttactg tatttttcat aataatatct tacctcctgc
    10681 atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa ttcatagtca
    10741 cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt gattggatca
    10801 gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta catttcataa
    10861 cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat gtcatgcata
    10921 tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat gatctcttct
    10981 ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc tggatttgga
    11041 aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta atcgttcatt
    11101 tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag aatacttgga
    11161 tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc ctgccactga
    11221 ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg ccatgagcca
    11281 tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca taaataatac
    11341 caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc attttttatg
    11401 atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt cggatgccac
    11461 tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat gattttatct
    11521 ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac tcttttttct
    11581 tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc ccacagtgcc
    11641 acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata ttgctgttga
    11701 tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt tttggctact
    11761 tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc taagcgaggt
    11821 ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac atctctgaag
    11881 atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact tcttttgtaa
    11941 acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa ttcagacgga
    12001 gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa cattgcttgt
    12061 tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc taatcgaaaa
    12121 gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc ttccgcctct
    12181 ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca cggatacatc
    12241 gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg aacagtgatt
    12301 tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg taccgaagga
    12361 tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga ggatgtggtt
    12421 tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga ttggttcact
    12481 ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt ttccttctct
    12541 ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa tcggggaagt
    12601 aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc tgttcctgat
    12661 aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt tgagctgaag
    12721 ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca aacatccatg
    12781 cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac tggatcaccg
    12841 gacgaatcgt ttgataggat ataatttggt cttttacttg cttttcttct tcaggagaca
    12901 gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct cctcttgttt
    12961 tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga gctgtgatgg
    13021 acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc actgcatccg
    13081 tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca aaccgtccac
    13141 ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa tgttcaagta
    13201 gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt gggtcaaggg
    13261 taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag tcaagaggaa
    13321 tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct aaccttgtaa
    13381 aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc tgcataagaa
    13441 aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt tcaaaccata
    13501 aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat ccatttgtca
    13561 acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct ctttttccaa
    13621 accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca tcaatgatcg
    13681 ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg accagaatcg
    13741 gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac tcatggtgga
    13801 tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca ggtgtttgaa
    13861 aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca ccgacgactc
    13921 tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa taaacaaatt
    13981 gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa gcaataaagt
    14041 tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt ccagataaaa
    14101 cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca atgacttcat
    14161 caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct agtttgattg
    14221 tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta tcagtaaata
    14281 tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa atcaccaacg
    14341 tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc tctggcttcc
    14401 catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca ttcttgcgct
    14461 gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat tcttgcggta
    14521 gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa aatccacgat
    14581 ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca aaataaatat
    14641 gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta tttcgtaaat
    14701 ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt ctatgcgtaa
    14761 tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat aattcctgta
    14821 tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg agtgtgtaaa
    14881 aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta cagtttcccg
    14941 tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac ggatagtaga
    15001 ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa agagaatgcc
    15061 ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata tctattacaa
    15121 acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga tgaatcgaat
    15181 ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag gtgcggtgat
    15241 cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa acatgcaaat
    15301 agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg cagatcttga
    15361 acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa gaatagccta
    15421 tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt ctataaatga
    15481 aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa aatatatgtc
    15541 ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta atctattaga
    15601 aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg caattggtgt
    15661 ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg tagcaagctt
    15721 tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc aaatcaatgc
    15781 gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg gtatacggga
    15841 cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt ttatcccaag
    15901 aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca tactcaaaac
    15961 gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat gtctgctggt
    16021 aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta atctcttttt
    16081 ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac cgattttcta
    16141 atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac gttggcggaa
    16201 attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc gttttccaat
    16261 cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata aatcgatcat
    16321 ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa atggttcctt
    16381 tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac gcatcattcg
    16441 tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca ccaaaaaata
    16501 attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct ttgatcagtt
    16561 cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca taattcatcg
    16621 caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg acacccgatt
    16681 tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca ggccccttgc
    16741 tgttttttgg atagtagatg tcataagtat tctctttata cttggactca tacgtcaaat
    16801 ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca gtgatttgaa
    16861 cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa agctggaagg
    16921 caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg ataaatttaa
    16981 gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg attataccat
    17041 agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa acttcttctg
    17101 caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat tttcttcttc
    17161 tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca gatatcgtgt
    17221 cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat ttttcgttac
    17281 agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga acaaaactcc
    17341 cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga gtaaaataat
    17401 gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa tttccgcgtc
    17461 aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc tttttgatca
    17521 atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc attctgaccc
    17581 agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat aatgtagact
    17641 aactaaaaag actaagaaac aataacaaaa gttaacactt cttagtcttt ctttagcacg
    17701 tctatttatt aaagatttta gggccttttc tcatgttttt acgatcattc ttaggtgttt
    17761 ccatttgttt ggatcggcct ccaccttgtt tctttttgat caattctttc attttttctt
    17821 tttggtccat ttttattctc ctttaacgaa tgaagcgact aattcgatat gatgggtttg
    17881 cgggaaaaga tctacaggct gaactttctt cacggcataa cctgcttctg caaataactt
    17941 agcatctctt gcaaaagttg ccggattaca tgagatatag acgattcgtt ctggtgacat
    18001 ttcgattgct gattcaataa aatgatgatc taatcctttt cgtggagggt caactacgat
    18061 cacagatggt ttgatccctt tttcttgcca ttttttcata actttttctg cttttccgac
    18121 ttcataatga acattttcga ttttattatt taaagcattg aactgtgcat cttcaattgc
    18181 ttctggaata acttccatcc catacacctt atccactctg tctgctaaag acaagccaat
    18241 cgtacctatt cctgaataag cgtcgatcac aatgtctttt ttatctaact cggcaaactc
    18301 gatggctgtt tggtacagat actctgcttg ttgagtattt acttgataaa atgactttgc
    18361 tgagattcga taggtttttc ctaatagctg atcttcgata tagtctctgc catataaaac
    18421 tttttctttg tctcctaaaa tcacatttgt tttttcttca ttgatatttt ggatgaccga
    18481 aaccacttct ggcaattctt catgaatagc ttcagcaatt tccttggatt taaagaattt
    18541 ttctttacga gtaactaaaa cgaccatcat ttcatgggaa taatgaccac gacggataat
    18601 gatgtgacga agctgtcctt catttttttc ttcattatat cctcgtactt ggaaccgttg
    18661 caagatatct ctgactactt tgattgcttg atcgatgaca ggatcttgga tataaaaatc
    18721 ttcgataggg actagatcat ggctattctt tttgtaaaaa ccggtggcta actgtccatc
    18781 gatacggcga acagggatct gtgctttatt acggtagccg aatggttctg ccattccgac
    18841 cgttggaagt acaggaactt ctggcatctt agcaatcttc gccattacat gctctacttg
    18901 tttttgtttg aagacaagct gttgttcata cttcaaatgg gctaaaggtg cgatacctat
    18961 gcgaagtaat gcattgtttt ctacaggctg acgatcaggt gattgtgtaa taaatgtttc
    19021 gacttttcca tatccaaact tattgcctgc ttttactact agtatttcta cttcttctcc
    19081 agggagtgca ttttcgataa ataatggata accatcgatt tttgctaccc ccattccttc
    19141 ataactcaag tcgacgattt cgactgtata tcgttcgttt ttctttacta tttgctgttc
    19201 tgtcatattt tgctccttta agcctatttt tcaatcctac tgaactctac tataaatcag
    19261 c
//
LOCUS       DASJON010000035        19078 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJON010000035 DASJON010000000
VERSION     DASJON010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.035"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..245
                     /locus_tag="U3989_000930"
     CDS             6..245
                     /locus_tag="U3989_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714394.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="U3989_000931"
     CDS             312..521
                     /locus_tag="U3989_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714395.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="U3989_000932"
     CDS             581..1252
                     /locus_tag="U3989_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1714396.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="U3989_000933"
     CDS             1305..3281
                     /locus_tag="U3989_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1714397.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="U3989_000934"
     CDS             3294..4049
                     /locus_tag="U3989_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1714398.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="U3989_000935"
     CDS             4046..4870
                     /locus_tag="U3989_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714399.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5171..7270
                     /locus_tag="U3989_000936"
     CDS             5171..7270
                     /locus_tag="U3989_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1714400.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="U3989_000937"
     CDS             complement(7308..9365)
                     /locus_tag="U3989_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEN1714401.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="U3989_000938"
     CDS             complement(9479..9691)
                     /locus_tag="U3989_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714402.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="U3989_000939"
     CDS             9878..10345
                     /locus_tag="U3989_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714403.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="U3989_000940"
     CDS             10342..10584
                     /locus_tag="U3989_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN1714404.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="U3989_000941"
     CDS             10602..10904
                     /locus_tag="U3989_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714405.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="U3989_000942"
     CDS             10976..13030
                     /locus_tag="U3989_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714406.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="U3989_000943"
     CDS             13030..15642
                     /locus_tag="U3989_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN1714407.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="U3989_000944"
     CDS             15639..18170
                     /locus_tag="U3989_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714408.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="U3989_000945"
     CDS             18222..18554
                     /locus_tag="U3989_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714409.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..>19078
                     /locus_tag="U3989_000946"
     CDS             18555..>19078
                     /locus_tag="U3989_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714410.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILG"
BASE COUNT         6854 a         2966 c         3803 g         5455 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagg
//
LOCUS       DASJON010000036        18919 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJON010000036 DASJON010000000
VERSION     DASJON010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.036"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(46..1365)
                     /locus_tag="U3989_000947"
     CDS             complement(46..1365)
                     /locus_tag="U3989_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1714411.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1362..2015)
                     /locus_tag="U3989_000948"
     CDS             complement(1362..2015)
                     /locus_tag="U3989_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1714412.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2303..2823
                     /locus_tag="U3989_000949"
                     /pseudo
     CDS             2303..2823
                     /locus_tag="U3989_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2725..3441)
                     /locus_tag="U3989_000950"
     CDS             complement(2725..3441)
                     /locus_tag="U3989_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEN1714413.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3565..3750)
                     /locus_tag="U3989_000951"
     CDS             complement(3565..3750)
                     /locus_tag="U3989_000951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714414.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3977..5250
                     /locus_tag="U3989_000952"
                     /pseudo
     CDS             3977..5250
                     /locus_tag="U3989_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5357..6388)
                     /locus_tag="U3989_000953"
     CDS             complement(5357..6388)
                     /locus_tag="U3989_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714415.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6395..7225)
                     /locus_tag="U3989_000954"
     CDS             complement(6395..7225)
                     /locus_tag="U3989_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714416.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7222..8037)
                     /locus_tag="U3989_000955"
     CDS             complement(7222..8037)
                     /locus_tag="U3989_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1714417.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8034..8858)
                     /locus_tag="U3989_000956"
     CDS             complement(8034..8858)
                     /locus_tag="U3989_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEN1714418.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8848..9948)
                     /locus_tag="U3989_000957"
     CDS             complement(8848..9948)
                     /locus_tag="U3989_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1714419.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10126..10911
                     /locus_tag="U3989_000958"
     CDS             10126..10911
                     /locus_tag="U3989_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEN1714420.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10944..11327
                     /locus_tag="U3989_000959"
     CDS             10944..11327
                     /locus_tag="U3989_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1714421.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11403..11534
                     /locus_tag="U3989_000960"
     CDS             11403..11534
                     /locus_tag="U3989_000960"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1714422.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11503..11739
                     /locus_tag="U3989_000961"
     CDS             11503..11739
                     /locus_tag="U3989_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1714423.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11817..12788)
                     /locus_tag="U3989_000962"
     CDS             complement(11817..12788)
                     /locus_tag="U3989_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN1714424.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13440..15062
                     /locus_tag="U3989_000963"
     CDS             13440..15062
                     /locus_tag="U3989_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEN1714425.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(15348..16724)
                     /locus_tag="U3989_000964"
     CDS             complement(15348..16724)
                     /locus_tag="U3989_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714426.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(16724..17380)
                     /locus_tag="U3989_000965"
     CDS             complement(16724..17380)
                     /locus_tag="U3989_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714427.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(17390..18667)
                     /locus_tag="U3989_000966"
     CDS             complement(17390..18667)
                     /locus_tag="U3989_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714428.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(18686..18847)
                     /locus_tag="U3989_000967"
     CDS             complement(18686..18847)
                     /locus_tag="U3989_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN1714429.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         5835 a         3899 c         3060 g         6125 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
     1381 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
     1441 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
     1501 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
     1561 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
     1621 ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
     1681 gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact
     1741 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
     1801 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
     1861 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
     1921 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
     1981 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctataca
     2041 ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt
     2101 gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaaaa
     2161 aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt
     2221 ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc
     2281 accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga
     2341 taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc
     2401 gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat
     2461 tcgtattatc cgcaacggtt cctatacgac acgtactcag atgttgaaag tcaaagaaaa
     2521 gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt
     2581 ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc
     2641 gattctcata gaactgtacg aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt
     2701 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
     2761 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
     2821 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
     2881 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
     2941 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
     3001 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
     3061 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
     3121 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
     3181 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
     3241 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
     3301 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
     3361 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     3421 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     3481 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     3541 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     3601 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     3661 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     3721 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     3781 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     3841 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     3901 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     3961 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     4021 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     4081 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     4141 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     4201 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     4261 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     4321 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     4381 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     4441 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     4501 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     4561 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     4621 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     4681 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     4741 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     4801 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     4861 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     4921 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     4981 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     5041 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     5101 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     5161 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     5221 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     5281 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     5341 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     5401 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     5461 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     5521 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     5581 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     5641 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     5701 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     5761 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     5821 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     5881 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     5941 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     6001 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     6061 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     6121 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     6181 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     6241 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     6301 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     6361 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     6421 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     6481 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     6541 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     6601 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     6661 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     6721 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     6781 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     6841 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     6901 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     6961 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     7021 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     7081 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     7141 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     7201 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     7261 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     7321 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     7381 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     7441 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     7501 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     7561 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     7621 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     7681 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     7741 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     7801 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     7861 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     7921 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     7981 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     8041 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     8101 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     8161 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     8221 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     8281 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     8341 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     8401 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     8461 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     8521 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     8581 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     8641 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     8701 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     8761 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     8821 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     8881 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     8941 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     9001 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     9061 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     9121 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     9181 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     9241 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     9301 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     9361 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     9421 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     9481 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     9541 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     9601 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     9661 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     9721 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     9781 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     9841 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     9901 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     9961 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
    10021 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
    10081 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
    10141 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
    10201 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
    10261 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
    10321 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
    10381 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
    10441 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
    10501 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
    10561 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
    10621 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
    10681 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
    10741 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
    10801 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
    10861 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
    10921 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
    10981 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
    11041 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
    11101 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
    11161 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
    11221 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
    11281 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
    11341 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
    11401 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
    11461 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
    11521 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
    11581 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
    11641 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
    11701 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
    11761 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
    11821 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
    11881 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
    11941 aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat ccataagata
    12001 aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag caaatgagga
    12061 accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg cgctggcgtg
    12121 tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata gcagtggtac
    12181 ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc aatcgtcagc
    12241 agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg cacttcgcag
    12301 ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt atctaagccg
    12361 gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa tgtatccaca
    12421 tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc cagtacggta
    12481 ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc ctgaaccagt
    12541 gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac agcatccttc
    12601 tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt ttcaatctgc
    12661 ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc ctttaaataa
    12721 acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct taatactgtt
    12781 ccttccatat agatttttga attacacact agtttaaact aaaacacaca aggaaaaact
    12841 taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat agactggtca
    12901 aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat tttaatattc
    12961 taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca ctatatcact
    13021 agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat
    13081 tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac gctgagagta
    13141 aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt tatttcagat
    13201 taccttaaat gattccattt actttttatc tcattttttc ctactagtta gtatcaaata
    13261 ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca gctaaaacct
    13321 tgtcattaca gcaaaaaatc acgttatcca gactgtagac aaagtccatt ttatggatga
    13381 tgtctacagt cttttctttt ataatagatt tatgaaaacg aatgcaagga gtgatagcta
    13441 tgatgtctaa aaacaatacg aatggtagaa atcaatttgc gatgcttacg attgatgatc
    13501 tagttcctca agatcatctc gttcgaaaga ttgatgccgc gcttgatttt gaatttattt
    13561 atccaatcgt cgaagccact tactccgatt taggtcgccc aagtatcgat ccagtgattc
    13621 tgatcaaatt ggtgtttatt cagtatttat tcggtatccg ttccatgcga caaaccatta
    13681 aggaagtcga taccaatgtc gcttaccgct ggtttttagg ctactccttc gaggagaaaa
    13741 tccccccttt ctcaactttt ggaaagaatt atgttcgccg atttagagaa acgacggtgt
    13801 ttgaggatat ttttgcctat attttagaac aagcagttaa agcaggtttt gtaacagagg
    13861 ataatttgta tctggattct acccacatca aagctaacgc caataaacat aagtttacga
    13921 aagaaatgac ccatgatgaa gcgaaagcct atcaggatga attagaagat gagatcaatc
    13981 gtcaacgaat agaggctgga aaacgccctt ttactttgga cttagaaaag gaagtgaagc
    14041 tgaaagaaag aaaaataagt aaagctgatc cggaaagcgg ctactacgtc aaaggcgaac
    14101 gagaaaaaca atttgcttat tctgtccata cttcatgcga cgacaatgga tttattctta
    14161 gcacaatcat cactcctaga aatattcatg acagtcaagt cgcctttcag ctcgtcaaac
    14221 aatcgaagag actttttcct gaaatcaatt gtgttgtcgc tgatgctggg tataagacac
    14281 ctaagtttgt tcactttttg actcatttga accttcgacc ttgtttgcct tattcaaggc
    14341 ccaaagggaa aaaggagcta ttatcaaaaa atgagtttct ctatgacgag tattttgatt
    14401 gttatatttg tccgcaagat caaatgctcg ctttttcaac ggttactcga gaagggtacc
    14461 gagaatacaa atccaatcca aaagaatgtg tgaactgtcc tctgctaaac caatgtacca
    14521 taagtaaaaa tcatcaacga gtcatcacga gacatgtttg gggagatctg atggatgaag
    14581 tggaacatct tcgcctaaca gatctgaata aatcaattta caaaaaacgg aaacaaacga
    14641 ttgaacgaat atttgcggat gcaaaagaaa agcatgggat gcgttggacc aagtatcgag
    14701 gcttggaaaa agtcgcgacg catacgatgc tagtgtttgc tgcaatgaat ttaaagaaat
    14761 tagccacctg gctatggaaa ggcaaggagc ctttattttt ttgttcaaaa attcgaaacg
    14821 aagtagataa aaagctgttc caagctcgag ttacgagttt ggaacagctt ttgtctacag
    14881 tctgcgatgt cggagacatc tcgttttcta ttaggaatca actcaggttc aaaagaagcc
    14941 tcccggtctc tgggaacatc aatgtttact ttaccaaagc tcgttttaag cgtcttattg
    15001 ccataaccgt ttcgtcgatt gtcatgtttc tttggctctt tagaatgggc gtcataccct
    15061 aaatggttat tcaattctcc ttgaagcatt ttttcaaata agggaccaaa cacatctttt
    15121 aaaacatctt gcatatcctc tacagattcg ggttgataag tattcaaaat ggattcagct
    15181 aacttctcag catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
    15241 attttagaaa aataaaaacc gtgaagcaac cacccgccta ggattgatgg ttacttacac
    15301 ggtttatatt acactctcat ttttggtgct aaaaagcacc gaaaatttta attattgtca
    15361 attaataatt cttttggatt ccttcttaat atattgaatg aagaaataat caatgataca
    15421 agaagtacta aactcataaa tataactaca taaatcatga atttaggtaa tacattgata
    15481 tctaaacttg ataatgtctt gttaaatcct tcagcttctg ccccaccacc taattggcta
    15541 gatgcagatt gtttagctat ttgtttagct atatcaccag taaccttatt caatatatta
    15601 ttcccaagct tgtcagctgt atattgagct aagaaatagg aaccaacgaa tgcggggatt
    15661 gatataaata ccatctcaat taggaattga ccaaaaattt ctaattttga tatacctaat
    15721 gatagtaata ctgctatttc tttctttcta gcattcatcc ataagaataa taataatgaa
    15781 actacaactc ctgcaaatat taatgagcca aaaaataatt tatttgaaat tgagtaaata
    15841 cctgatatag attgttgtaa tgcagggtaa tttgatgaac ttttaatcaa attatattct
    15901 ctccaattta tatctaattt cccaagatct tttattacac tatcaagatt cttatcacct
    15961 tttacaaaga atgttgcatc ttggtataca gctgtatctt cggtattacc ataaacttta
    16021 gcagctgtat gaatatcagt gattaatgta ttttcgtata gttcttgtgc tgcacttact
    16081 acaccgctat tatgcccatc gaatagacct ttaatttcta cttctactgt ttcatctgca
    16141 cctttctcat tatcagcatc aaataaatta gattttattt ttatcttatc tccaacttta
    16201 aggttatttt tttcagctaa atctttatgc attaagattt tatttttatc ttcattttct
    16261 aaatgctttc cttctactaa tttatatgct tctgatacaa attttgtttc ttttgttgag
    16321 tcgttaaccc cagttaacat aactgttctt ttaaaattct ttgctctttc aggtgattga
    16381 ttcgcaagag tctcttgagt ttcaataata tcataatcaa ctaaatctgc aacactgttt
    16441 attcttttta cataggagtc tatactatct gtttgagata tctttttaat atcttcacct
    16501 tttacatttc ccccacctct aggtgttcct ggatttactt gtctatttat ctccatagaa
    16561 aaactatttg ttatattagc aaatgtttct tttgaagctc tgtccgtagc atctttaata
    16621 gataaactaa tgagacttaa agttgacata gataaaataa ctaatatgat aattaatgat
    16681 tttaaacttt ttctagttac ataagcaaat gcatttttta tcattattca acctccaaat
    16741 tcattttatt tacttttttc aactttttac cacttagttc taaaatgata tccgcagaat
    16801 ctgctacttc cttactatgt gttacaacta ttacacattt atttctatct ttagctaatg
    16861 tctttaatat attaattatt tctccagcag taacactgtc tagattacca gtaggctcat
    16921 cagctagtat tattggagca tctgatacca atgctctagc aatagctact ctttgttgct
    16981 gtccaccaga taatttcata acatttcttt ttatttgttt tttatctaaa cctagttcaa
    17041 acaagatact ttcatctgct gatttattta ctaatctaat attttcaatt ggtgataaat
    17101 aatctattaa attatagttt tgaaatacta aagatatatt attttttcta tgattactat
    17161 atcctttatt ttgtatatct tcattcttaa acaatatttt tcctgtttga ggtttatcaa
    17221 gtccagcaag taaggaaaga agtgtagatt ttcctgttcc tgactttcct actatcgcat
    17281 aaaacttccc aagttcaaat ttttgattta ctcctgacaa aactttttct ttagaatttg
    17341 cataactata tgctacattc tttatttcta atatatccat tattttctcc taacttattt
    17401 ttgataagat ttctttaggc ttcttgatta acattaatga agaagcaaat acaactgata
    17461 aaacaataat acttattaat attaaataac tttgtccaag tgttgttata tttgacataa
    17521 aactactatt atttattaaa cttccaccgg aaatcattga atcctctgag ttaatgaatc
    17581 catctacaat tacttttagt aatacatttc ctaaaaataa ggaagatatt atacttggta
    17641 tcgatatgaa tattaactcg aatataaatt gcattataat ttgtatctta gatgttccaa
    17701 tagataagaa tatacctatt tcataaattc tttcccttaa ccatagaatc aagattaatg
    17761 aaagaacaac catcccacct aacataatag aataagtcat tacttttatt atatatttta
    17821 ttccactcac tgactctaaa gactcttcaa acgcattaga atctttttca acaaaatact
    17881 ttgactcatc aattttaagc tctttcaatt tgtttaaggc taagtctgta gattctgcac
    17941 taccagaata cattaaaatt ttatttgcaa tttcattatt ttctgaatta tttaatatct
    18001 cttggcttgt tgaataatct acaaacacca tattttcact aaaatcagaa gataatcctg
    18061 tatatgtttc ctgtttttta ccagaaaaga tccctataat tttaaattta tgacttttta
    18121 tttttccact tttttcaata tctagtaatt caagatcaac ttcatcacct aattttagat
    18181 tgttttgttt agcaaattct tcatgaacaa taattgaatt cttatcattt tcttctatat
    18241 ttttaccttc tttaattgta aatactccac tactaaataa aatatttctt ttagtattat
    18301 ttgtagcttc gagtgaaaca acattcttaa attcgtcaga taaatcttct ctatttattc
    18361 tttgttctcc gctaactact ttagcatctt ttagttttgc taatccatca tattgaatta
    18421 ttttttcttc aatttctttt aatttttcaa tatctttaaa ttgattaaca ttaaaatatt
    18481 taccatcttt ttttgttatt gatattgaag aattagaact ttcatataaa gccttttcta
    18541 tttcattgct tgatttcatt attgttaaac aagaatacaa gcaagaaaga actatagtta
    18601 atatgataaa aattattaaa gttctatttt tctttcttgt aatatatgct atagcatttt
    18661 ttatcacttt cctaactact cctctctatt ttcttactcc ataactaatt gcatccactg
    18721 gacaagcatt tttacatcgt ccacatctga tacactccag atgattgcaa ttctcaactg
    18781 aatctttatt catttggcaa acttttttac attttccaca attaatacac ttatccttat
    18841 cctgaatcgg ctacactaga ctagacagaa aaaataaggt gtgtagaata ataaaaaaca
    18901 cactggagga tttttcatg
//
LOCUS       DASJON010000037        18627 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJON010000037 DASJON010000000
VERSION     DASJON010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.037"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(171..740)
                     /gene="thiT"
                     /locus_tag="U3989_000968"
     CDS             complement(171..740)
                     /gene="thiT"
                     /locus_tag="U3989_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN1714430.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(806..908)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(914..1885)
                     /locus_tag="U3989_000969"
     CDS             complement(914..1885)
                     /locus_tag="U3989_000969"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1714431.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(1979..3352)
                     /gene="glmU"
                     /locus_tag="U3989_000970"
     CDS             complement(1979..3352)
                     /gene="glmU"
                     /locus_tag="U3989_000970"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN1714432.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(3855..4673)
                     /gene="purR"
                     /locus_tag="U3989_000971"
     CDS             complement(3855..4673)
                     /gene="purR"
                     /locus_tag="U3989_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN1714433.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(4840..5664)
                     /locus_tag="U3989_000972"
     CDS             complement(4840..5664)
                     /locus_tag="U3989_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1714434.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5637..6317)
                     /locus_tag="U3989_000973"
     CDS             complement(5637..6317)
                     /locus_tag="U3989_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1714435.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6340..7278)
                     /locus_tag="U3989_000974"
     CDS             complement(6340..7278)
                     /locus_tag="U3989_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN1714436.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7523..8386)
                     /locus_tag="U3989_000975"
     CDS             complement(7523..8386)
                     /locus_tag="U3989_000975"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN1714437.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(8528..11101)
                     /gene="mprF"
                     /locus_tag="U3989_000976"
     CDS             complement(8528..11101)
                     /gene="mprF"
                     /locus_tag="U3989_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1714438.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11242..12048
                     /gene="proC"
                     /locus_tag="U3989_000977"
     CDS             11242..12048
                     /gene="proC"
                     /locus_tag="U3989_000977"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN1714439.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12127..13683)
                     /gene="rny"
                     /locus_tag="U3989_000978"
     CDS             complement(12127..13683)
                     /gene="rny"
                     /locus_tag="U3989_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN1714440.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14094..15143)
                     /gene="recA"
                     /locus_tag="U3989_000979"
     CDS             complement(14094..15143)
                     /gene="recA"
                     /locus_tag="U3989_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN1714441.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15252..16493)
                     /locus_tag="U3989_000980"
     CDS             complement(15252..16493)
                     /locus_tag="U3989_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN1714442.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16733..17311)
                     /gene="pgsA"
                     /locus_tag="U3989_000981"
     CDS             complement(16733..17311)
                     /gene="pgsA"
                     /locus_tag="U3989_000981"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN1714443.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17520..18443)
                     /locus_tag="U3989_000982"
     CDS             complement(17520..18443)
                     /locus_tag="U3989_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1714444.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         5293 a         4140 c         3251 g         5943 t
ORIGIN      
        1 gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt ggtagaagat
       61 gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa taaaaaggaa
      121 acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat ttacagctcc
      181 tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa taaaacgatg
      241 atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga aaagagccaa
      301 ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat aaaatgccaa
      361 aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct aatttgtttc
      421 gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc tgcgcctgca
      481 aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata ataaacttgt
      541 cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc ctttgtccct
      601 cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa actgctgccg
      661 atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt tccttctacc
      721 caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt cacaaaggag
      781 gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg ctcgcataac
      841 cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg actccccttt
      901 gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt ttgttttcat
      961 ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg attttgtcga
     1021 ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg atagctgaat
     1081 cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa gcatacacac
     1141 tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct gctgtatcga
     1201 tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata ttcattactt
     1261 ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat ttcaaaaatt
     1321 ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg acaacatcat
     1381 cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc atcaaatgat
     1441 cgactggaat atcaaagaaa ccttggattt ggactgcatg aaggtccaaa gtcagcattc
     1501 ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt ggttcccgtg
     1561 cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg ttgattgttt
     1621 ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag ttatcattga
     1681 ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt tcttcaatgt
     1741 ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac tcgacgccga
     1801 ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa atttttaatc
     1861 ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca actctagaat
     1921 tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg caaaagtctt
     1981 agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt gctctcgcaa
     2041 ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca atcgaactgt
     2101 ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag ctgtggtcgc
     2161 cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca ccacatccga
     2221 cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg actttagttc
     2281 ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg ttttcgccaa
     2341 tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc ttgacatggc
     2401 tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg tctacgattt
     2461 ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg atttgtacac
     2521 ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat gtcgttgctg
     2581 aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt ctcatgatct
     2641 catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca aaatcatccg
     2701 tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata tctgttagat
     2761 agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac aaagcttcgt
     2821 tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct ggtgttgcat
     2881 ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt cgtccatatc
     2941 ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt ccttggtgat
     3001 attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca ctgataacta
     3061 acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca tgtcctgttc
     3121 ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct ttaacttgtt
     3181 ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg acccgattaa
     3241 tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat aattttgact
     3301 tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc aactgcgtca
     3361 ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt tcaacaaaac
     3421 aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac cataacgggc
     3481 ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc acactactca
     3541 taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta aacggtaggc
     3601 ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag ctcaataaac
     3661 ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac aggacgctga
     3721 tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta agccaaattt
     3781 atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa actaagcggc
     3841 tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat atttttcgtt
     3901 tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc gcgatgaccg
     3961 ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa ttcttcaatc
     4021 aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac tacaaggaca
     4081 cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct ttcagaagat
     4141 ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc ccggcgtaca
     4201 ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac gccttttgtg
     4261 gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat gattcttcca
     4321 acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata tcctcctgga
     4381 agcagacggt cttgttctga tagacgttct gctagagatt caatatattt tttcgcctct
     4441 tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat tgtttccaag
     4501 gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc actgatagaa
     4561 gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc atgcgggtga
     4621 ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt cacctattcc
     4681 aactcctttt aagaaatttt atcgttttac attatataag tttttcttca aaaattcgag
     4741 cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag aagcctataa
     4801 aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt ttcttctgaa
     4861 ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa agactaatgt
     4921 gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg acagcatccc
     4981 tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc ctaatcgcat
     5041 accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg caatcgggat
     5101 catcacagca atagccacac cagtaatcac attgaacatc atcgacatca aatgaacggg
     5161 tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg gccgtttgaa
     5221 taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga cttgctccca
     5281 tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg aattccctcc
     5341 gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa tggcaatgga
     5401 gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag ctgctaatac
     5461 aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta acgctacccc
     5521 tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga tcactaaaaa
     5581 cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg ctcttctcat
     5641 gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg gatctgtctg
     5701 tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga tttcccatgg
     5761 acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc atccatacct
     5821 gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg cgctaaactg
     5881 attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat atcccacatc
     5941 cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag acgcttgaac
     6001 cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact aggaaaacct
     6061 gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt tccttcttga
     6121 tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa cgatgctttc
     6181 accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc acccgcatct
     6241 acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt caaatcagat
     6301 atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac tttcttttaa
     6361 agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga tttgttcatt
     6421 agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat tcgacaatgt
     6481 ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt tgtttttctt
     6541 cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg gtgaaacgcc
     6601 cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag cagcatgctg
     6661 agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt gatccagtgc
     6721 ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt tatctggata
     6781 tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata ccggatcgag
     6841 ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt cttcatgttc
     6901 gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca tcaaggtctg
     6961 tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg aactattata
     7021 cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt cgaaatcatg
     7081 cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta cgatctcttt
     7141 tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt ttttgtctgc
     7201 ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta ccgtagctgt
     7261 caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat tttacgattt
     7321 attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc catcaatttc
     7381 tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct
     7441 agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat
     7501 cacctatttt acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa
     7561 aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa
     7621 cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt
     7681 tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt
     7741 ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt
     7801 gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc
     7861 ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc
     7921 ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt
     7981 ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc
     8041 ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat
     8101 tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag
     8161 cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa
     8221 tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca
     8281 tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat
     8341 ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag
     8401 tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga
     8461 ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta
     8521 gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt
     8581 ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag
     8641 caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga
     8701 aggaatagaa aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag
     8761 aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc
     8821 ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt
     8881 ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg
     8941 tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat
     9001 atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt
     9061 gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat
     9121 ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt
     9181 ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat
     9241 gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat
     9301 ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat
     9361 ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt
     9421 cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg
     9481 tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct
     9541 tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca
     9601 aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg
     9661 ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca
     9721 aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc
     9781 gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat accaatatca
     9841 actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg
     9901 taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa
     9961 agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt
    10021 gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc
    10081 ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg
    10141 gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc
    10201 ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa actactgcta
    10261 catcttgccg aacagacaat tgactcattc ctaagatcat caaaacgtca aaagtcccca
    10321 ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga
    10381 tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg
    10441 ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact
    10501 cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac
    10561 taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga
    10621 catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt
    10681 ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca
    10741 gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg
    10801 cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga
    10861 tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga
    10921 gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc
    10981 cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca
    11041 gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca
    11101 cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt
    11161 ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct
    11221 aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa
    11281 gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc
    11341 agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa
    11401 agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc
    11461 attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt
    11521 gcttcaggaa aatcattaat agatttagaa acagcgcttg aagatcctca ggcctccatc
    11581 gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa
    11641 aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc
    11701 agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca
    11761 cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg
    11821 tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc
    11881 ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca
    11941 actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga
    12001 atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt
    12061 tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat
    12121 tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg
    12181 tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc
    12241 gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac
    12301 tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg
    12361 acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc
    12421 tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc
    12481 gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg caccgatctc
    12541 aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc
    12601 acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc
    12661 aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc
    12721 gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc
    12781 cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat
    12841 acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc
    12901 tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa
    12961 atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg
    13021 acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc
    13081 tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc
    13141 tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc
    13201 agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag
    13261 ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg
    13321 ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga
    13381 gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa
    13441 ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc
    13501 cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat
    13561 gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac
    13621 cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac
    13681 cataaaatcc acctccatac tatctttttc atttttatca tctatacagc ttctatgata
    13741 aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa
    13801 ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc
    13861 tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc
    13921 tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt
    13981 tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca
    14041 ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa tttttattct
    14101 tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca
    14161 ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc
    14221 atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa
    14281 gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt
    14341 gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg
    14401 ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt
    14461 tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga
    14521 gtgatttctg gattcgcaaa catcacgcct actttttcac ggatttggtt gatgaaaata
    14581 gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa
    14641 cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact
    14701 aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca
    14761 gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc
    14821 acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc
    14881 gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa
    14941 ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg
    15001 acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc
    15061 aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag
    15121 gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac
    15181 atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat
    15241 attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc
    15301 gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg
    15361 tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt
    15421 ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg
    15481 gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca
    15541 atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact
    15601 tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc
    15661 gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa
    15721 ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat
    15781 gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc
    15841 acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct
    15901 tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt
    15961 agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt
    16021 ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa
    16081 aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg
    16141 gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat
    16201 tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct
    16261 tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt
    16321 gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga
    16381 taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc
    16441 acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc
    16501 cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg
    16561 agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa
    16621 aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc
    16681 cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat acttacattg
    16741 atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga
    16801 tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat
    16861 tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc
    16921 aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta
    16981 attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca
    17041 tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat
    17101 tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta
    17161 aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa
    17221 attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc
    17281 taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac
    17341 gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag
    17401 gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt
    17461 gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat
    17521 cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga
    17581 ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg
    17641 atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt
    17701 gtcccttgat aaacataggc attgtctact agcacaccta accatacacg attgacagca
    17761 gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca
    17821 gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct
    17881 actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat
    17941 ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc
    18001 caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca
    18061 ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa
    18121 gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca
    18181 tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata
    18241 aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc
    18301 aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc
    18361 aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca
    18421 gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg
    18481 tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc
    18541 tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg
    18601 tgactcggag ttaaacggca ggctcac
//
LOCUS       DASJON010000038        18504 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJON010000038 DASJON010000000
VERSION     DASJON010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.038"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="U3989_000983"
     CDS             99..746
                     /locus_tag="U3989_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1714445.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="U3989_000984"
     CDS             1139..3784
                     /locus_tag="U3989_000984"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN1714446.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="U3989_000985"
     CDS             4094..5407
                     /locus_tag="U3989_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN1714447.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="U3989_000986"
     CDS             5397..6050
                     /locus_tag="U3989_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1714448.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="U3989_000987"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="U3989_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN1714449.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="U3989_000988"
     CDS             6947..7147
                     /locus_tag="U3989_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1714450.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="U3989_000989"
     CDS             complement(7401..9080)
                     /locus_tag="U3989_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1714451.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="U3989_000990"
     CDS             complement(9082..9294)
                     /locus_tag="U3989_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN1714452.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="U3989_000991"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="U3989_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN1714453.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="U3989_000992"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="U3989_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN1714454.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="U3989_000993"
     CDS             13269..13466
                     /locus_tag="U3989_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714455.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="U3989_000994"
     CDS             13619..14104
                     /locus_tag="U3989_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN1714456.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="U3989_000995"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="U3989_000995"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN1714457.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="U3989_000996"
     CDS             14981..15508
                     /locus_tag="U3989_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714458.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="U3989_000997"
     CDS             15599..16336
                     /locus_tag="U3989_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1714459.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="U3989_000998"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="U3989_000998"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN1714460.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="U3989_000999"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="U3989_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN1714461.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DASJON010000039        18152 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJON010000039 DASJON010000000
VERSION     DASJON010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.039"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            334..2034
                     /locus_tag="U3989_001000"
     CDS             334..2034
                     /locus_tag="U3989_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714462.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(2136..2969)
                     /locus_tag="U3989_001001"
     CDS             complement(2136..2969)
                     /locus_tag="U3989_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN1714463.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3273..5438)
                     /locus_tag="U3989_001002"
     CDS             complement(3273..5438)
                     /locus_tag="U3989_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1714464.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            5698..7992
                     /locus_tag="U3989_001003"
     CDS             5698..7992
                     /locus_tag="U3989_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1714465.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            7985..8650
                     /gene="pgmB"
                     /locus_tag="U3989_001004"
     CDS             7985..8650
                     /gene="pgmB"
                     /locus_tag="U3989_001004"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1714466.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            8643..9650
                     /locus_tag="U3989_001005"
     CDS             8643..9650
                     /locus_tag="U3989_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1714467.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            9672..10691
                     /locus_tag="U3989_001006"
     CDS             9672..10691
                     /locus_tag="U3989_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1714468.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(10972..12339)
                     /gene="nhaC"
                     /locus_tag="U3989_001007"
     CDS             complement(10972..12339)
                     /gene="nhaC"
                     /locus_tag="U3989_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1714469.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(12448..13869)
                     /locus_tag="U3989_001008"
     CDS             complement(12448..13869)
                     /locus_tag="U3989_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1714470.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(14112..15989)
                     /gene="tdc"
                     /locus_tag="U3989_001009"
     CDS             complement(14112..15989)
                     /gene="tdc"
                     /locus_tag="U3989_001009"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1714471.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(16280..17536)
                     /locus_tag="U3989_001010"
     CDS             complement(16280..17536)
                     /locus_tag="U3989_001010"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN1714472.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(17648..17853)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         5459 a         3834 c         3286 g         5573 t
ORIGIN      
        1 atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta tgctttgagt
       61 aataagagcg ctagtcagaa aatagcttct catattttct aggctagtga gcttattacc
      121 gagaagcagc tgattgaaca ccgtttaccg agaggtcatg aaaaagctac tttgtttcga
      181 gtattaagaa aaattctgtt tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc
      241 tctccttaga actttcactt atgtttttct tgtaaaattc ataaatattt tcatgaattt
      301 tctaatttct tgcctacctc tcgcatagga tatatgatga atttatctaa agtttgcaaa
      361 ggagttcgac aattgaaaaa atatggccca tttttagcaa tttttttcat tgctttggca
      421 tttctcatgc ctcatttatt cacacataaa ctaattttgg gtgctgatgc cccattccat
      481 tacaatcggt tctatgatgc cgcgcaacaa attaaatatg gggatttcca ttattttcct
      541 tccctttatg gattccagca aagtggcagg atcgtgaatg ccgtttacgg cccatttttc
      601 gcttatttac aaggattgat cgtgttgctt gcaggtagct ggtttcatta tcagattctt
      661 tcaaatttca ttctttatct tatttcagga ttttctatgc tgattttact gagatatgtc
      721 aatgttcgaa attgggtaag tgtaccagta gccgttttat tcatgagtac gtattctatt
      781 caatactggt ctatcaacca aggattcact agctggggga cttgttttta tcctttatgt
      841 atgatcccta ttgctgattt cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc
      901 tcagtgggac ttatgacaca gatccattta ctgtcttcga tcatgttagc tttgatgtat
      961 atcccttttt atctttatta cttttttaac cagactgaaa aaggaaaagc actgcttcaa
     1021 ctgattaagt cggtcttgct ttattttgtt ctgacagcaa atatttgggc aggattagct
     1081 attgtttaca gtgggaataa gattcttcct ccctttgtta acagagaaat gagtcaaaag
     1141 gcgatagatc tggatggcag ttattggctc cattacccta aggcctttcc tatattgata
     1201 ttatcaggtt ttttgctttt tattttcttt caacaaaaga acaccttatt ccaaaaaatc
     1261 gtgttgtaca caagtcttgt tttctttttt ctttctacta gcttgattcc atggacgaca
     1321 ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt ttccatttcg atttttcgtg
     1381 ccatttacgc tattgcttct actttacctt gccctttcac tgaaccagtg gtctgaacaa
     1441 aagtggttcc gtcttttaag tatatttggc attcttattt tttcagcaca gacgatccag
     1501 aacttatacc agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg acatacttat
     1561 ctattcgata cgccggaaga agcaaggaaa acattcttca gcaaagatat gtctaccagc
     1621 ttgttcgttt tccaaaaaac cacacctgac tatctgccaa tctataagga gacaaaagac
     1681 aataaatacg accggtataa cgaagaaata ctagaaaacg aaacaacttt tataaaaaca
     1741 catccaggcg gtcttttgac aatcaagtgg aaaaacactg gggacaaaac tgttcatatc
     1801 cctgttatcg tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt gactgattat
     1861 gaagtaacag atatcgggac cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg
     1921 cttcattatc aaacgccaat ctatttctat ttcattcttt ctcttacttt gattggctgg
     1981 tttaccttgc tatgtttatt tatttatcat agatacaagc ttttacgtgc ttaggcaaaa
     2041 gggataaata catccgatca aaaaactctc caaacggtat aatcgtttgg agagttttat
     2101 tttaatcagt ttttttatgt tatatgttat attttttatt cgttcacatc taattcgatt
     2161 tccaccccgt agtgatcact gatgattgga ctatcgatcc catcaaaaat ccgttgatat
     2221 tttttaacct gcattccttc agggacgaaa ataaaatcta tacgcaattt ttctgtattt
     2281 tgttcccaac catctatttt cttttccaca gtagctgctt ttcctttttc attcgcgaca
     2341 acatacgaat cttgaagaag aaggcaactt tctcttacag tataataacc ttgagagtct
     2401 actggattat tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa aaagtagttt
     2461 tccaagtttt tccactcaaa ataaaagcca ctttttttat ctgtccacca agaaaagtgg
     2521 cagctcgaga caaaaatgtc ctgatcatct atctttgttt tcccaatcaa gatcttgcgt
     2581 gtctgataat cttctttctt ttgaaattgg gagacggtaa ggacctgact ttccaatgtt
     2641 tttttcgtaa gcagtgcatt tccttcttca tatttatcat aaccgatgtg gctcatttcc
     2701 caactccaaa aatactcttt tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa
     2761 ttatctttac ggataggtac ttgattcttt accggacaga acttcattag cttcgcaggt
     2821 tctacttctt cagattccag taattggttg atttcttgta aagcaatcac ttcataatct
     2881 tctaacagga tcttatctgc taattcttgc aattttttct ctggttctgg ctcaagccag
     2941 ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa
     3001 aagggttgtg acaaaatttt tgtcacaacc tctccagcaa atagtgttct cctgctaact
     3061 cctcagtaat aagctcaatg gaccaggagc tattttttgt ttggctgttt ttatcacttg
     3121 gtgcagggca gctttttcac gacctctcca ctaaacagta ttcaaactgc tgcttctcgg
     3181 taataagccc aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttatgac
     3241 ttgatgcagg acagcttttt cacaacctct ccttacattt taatggagcc gatcacttta
     3301 ccagctgtca ctgtacctaa ttgcttgatc actacttctt ctactttatc gtttgtaaat
     3361 acgatcatga tcgttggatc tttttcttcg ttttggatca catcaagatc catctcagct
     3421 aatttcgttc ctgttcctac ttcttggcct tctttgacag caatctcaaa agctggctga
     3481 ttcatttgga ctgtatcgat tcccatatgg atcaaaactt cgataccatc tttcgtttcc
     3541 agcccaattg catgtttgct agggaaaata cttgtcactt ttccagcgat cggcgcaaca
     3601 acttctcttg atgttggaat aacagcaaag ccgtcaccca tcattttttg tgagaatacc
     3661 ggatctttta cttcagataa aggaatcact tctccagaag ctgcggaaac tagttcttgt
     3721 tctataccta agtattgctt actacctgct tgtccatttc ctgtagaaac catttctggc
     3781 tctggaatct ctgctcctga ttgcagcaga tcttcgatat ctgatttcaa gacatctgct
     3841 tttggtccat aaactgcttg gactccattg tctttgacga tcaatcccat cgcccctgct
     3901 cgtttccagg cttcttcagt ccctactttt tctcgatcat ttacacttac gcgtaaccga
     3961 gtcatacaag catcgacatc tttgatattt tcttttcctc ccagcaaatg aatgatctgg
     4021 accacttgct gatctgctgt tcctgttccg ctgttagata caacggtttc ttcactgtca
     4081 ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga attttttgat caagaaattt
     4141 gctaagaaat acgtaacaac accaaaagcg atcacacaca acacgaagtt gataagatct
     4201 ccacctagac ccgctttgat agctaacggt gtccgagtca gcaattcgat attcccaaat
     4261 gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg cagccccttg gatcactgaa
     4321 taaatgacat ataatggcac agcagcaaac ataaacatga actccaatgg ttctgttaca
     4381 cctgttagaa agacagccaa cgctgctgaa aaatacattg atttatatct tgctttctta
     4441 tcaggatcga cattacgata catcgcaaaa gccattccca tcaagatacc tgaagaacca
     4501 atcatttgtc ctactttgaa acgagccggc gtccagttat gcaacacaaa ctcatattgc
     4561 gacatatccc ctgcaccttt aagattcact aaatcagtgg cccaagccaa ccataacgga
     4621 tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata aaatctcata cgatccgcca
     4681 agctgtgtat agttgatagg aatcgtcagc atatgatgca atccaaatgg caatagtaaa
     4741 cgttctaatg ttccaaataa aaacggtgct agaatcggtg cgctttcttg tgattctgca
     4801 atccataaac caaaattatt gattccagcc tgtacgtttg gccacaccac agcaagaatc
     4861 aatgccacga ttgttgacca taagataaca acaaacggaa caaagcgctt tccattgaaa
     4921 aatgacaaag catccggcaa ttttctataa ttatagtact tattataagc cattgccccg
     4981 acaaatccag cgatgatccc tacgaatact cccatattca aagctggtgc ttccaataca
     5041 cttgtaaaaa atcctttaac catgatcttc gtaccgaaca gagtgtgggt aaatgcatct
     5101 tcattcgcaa gcatgtcact tgtgacacca aagatagccc cagtgatccg attgattaaa
     5161 ataaatgaga tcccagctgc aaatgctccg cctgctcttt cttttgccca acttcctcct
     5221 attgctaaag caaataaaat atgaagatta ccaataattg cccaaccaat attttcaatt
     5281 actccgcccg ttgtgacaag cggcgtccaa tcaggattga tcaagggaat tgatttccct
     5341 atactgatca tcaacccagc tgccggcata actgctacca caaccattaa agcctttcca
     5401 aatttttgcc aaaactcaaa actaaatatc tttttcattc cgtttagctc ctttacgcaa
     5461 tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt tgtaaatgct ttatttttta
     5521 tttttctgtc tataccattc tataaaacct ataatacgtg tgttcccttc tataattttc
     5581 tcctaaaaat ttattttttt aagctttttt ctatgaaata ttttcttttg tctattgact
     5641 tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc tcattaggag gagaaaaatg
     5701 aaacagatca aacgactatt tgctattgat ccatggaaaa tcagcacgac acaattagat
     5761 aaaaaaaatc ttcgactgca agaatcttta acaagtattg gaaacggtta tatggggatg
     5821 cgcgggaact ttgaagaaac ttactcaggg gaccaccatc aaggtactta tttggcgggt
     5881 gtctggtatc ccgataagac acgtgtcggc tggtggaaaa atggttatcc agaatacttt
     5941 ggaaaagtga tcaacgcctt gaactttatc gcgatagata tttcgatcaa cgatcagctg
     6001 atcgacttgg cttctttgga accagaagac ttttactggg aattagatat gaaaaatggt
     6061 gtattatctc gtacattcac aatcactact tctactaata aagtacgcat ttccttcgaa
     6121 cgttttctga gcattgtcaa aaaagaatca gcttatatct atttaactat agaaatgctt
     6181 gaaggtagcg gaacagtcaa actcgtatca aaattggatg gaaatgttca aaacgaagac
     6241 agcaactatg atgaacattt ttggaatgag attgatcgtg gaactataga agaaatcggc
     6301 tatttgacga ctaaaactat tccaaacaac tttgggattg aacaatttac tgttactgca
     6361 gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg actatcacga agatacttta
     6421 gcattacatg gttgcttgtc tgcagaatta tctcaaggtg agacatggtc agtggaaaaa
     6481 gaaattatcg ttcttactag tagagatatt ccagaagcgc agcaagcaga aaaagctgtc
     6541 tcagaattaa cgaaccagac aaacaattat atccaagcaa aagaagagca aacagcggct
     6601 tgggcagaac gatggaagct tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa
     6661 ggtattcgat tcaatttgtt ccaactattc tctacttact acggcgaaga tgaacgattg
     6721 aatatcggcc ccaaaggatt cacaggagaa aaatacggcg gagccactta ctgggataca
     6781 gaagcgtatg ctgtccctct ttatcttgct ttagccaaac ctgaagtaac taagaatctg
     6841 ctgaaatatc ggcataatca actgccgcaa gcaatccaca acgctcagca acaaggatta
     6901 aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg aatgccataa cgagtgggaa
     6961 atcacattcg aggagattca ccgaaatggc gcgattgcct atgcaatcta caattatacg
     7021 aattacaccg gcgatacgga atatttgaaa aatgaaggac tggaagtttt agcagaaatc
     7081 gcacgcttct gggcagaccg tgtccatttt tcaaaacgag caaataaata catgatccac
     7141 ggtgtgaccg gcccaaacga atatgagaac aatatcaata acaattggta tacaaatacg
     7201 atcgcagctt gggtcttgcg ttacacgcga gaaaattatt taacttatca aaaagaagct
     7261 gctatcacta tcagcgaaga agaactagcc aaatggaaag atatcgcaga gaacatgtac
     7321 ttcccagttg acgaagaatt aggaatcttt gtccagcatg acacattctt agataaagat
     7381 ctcatgcctg tatccgatat tcccacaagc gatcttcctc tgaatcagca ttggtcttgg
     7441 gacaaaatcc ttagatcttg cttcatcaag caggcagatg tactacaggg gatttatttc
     7501 ttcaatgata cttttacaaa agaagaaaag caaaaaaact ttgatttcta tgaaccaatg
     7561 actgtccatg agtcttccct ttcgccaagt atccattcga ttttagcagc agaattagga
     7621 atggaaagca aagctgtaga aatgtaccag cgaacagctc gcttggattt ggataattac
     7681 aataacgaca cagaagacgg cttgcacatc acttccatga ctggtagctg gttgtcgatt
     7741 gtccaaggat ttgctcaaat gaaaactgcc catgatcagt tgagattcgc accatttctg
     7801 ccaaaaactt ggaaagctta ttctttccat atcaattatc gcggacgttt attgttcatc
     7861 tcagtcacag atgaacaagt tactttcgag ttattagaag gttctcctct agaaatttac
     7921 gtctatgaac agtcttattc tttagcggat cgtctagaaa taacgctaag aaaggaggaa
     7981 gcaaatgttt aaaggggtat tgtttgattt agacggtgtc attaccgata ctgctgaatt
     8041 ccattatcgt gcttggcgaa agcttggtca ggaaatcggc attacgatcg accacacatt
     8101 caacgaacag ctaaaaggcg tcagccgaga agactcactc tctttgcttt tagcttatgg
     8161 cggaaaagaa cactcttttt caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga
     8221 ttattattta gagatgatcc agacgatcga accaaaagat gtcttccccg gggtcgtgcc
     8281 tttactggat tctttaaaag aagaaaaaat caagattgct cttgcttctg caagtaaaaa
     8341 cgggccattc ctacttgaaa aaatgggtct tacaccttac tttgatgcta ttgccgaccc
     8401 ggcagaagca acaaacggaa aacctgcgcc agatatcttc ctccttgcag caaaagctgt
     8461 cggactaaaa gcagaagaat gtatcgggat cgaagacgcg caagccggta tccaagccat
     8521 cctctcaagt ggcgctcaac caattggcgt gggacgtgct gaagaccttg gagaggagat
     8581 cccgcttgtc cctgatacga cttttcttac gatcgattac ttgaaagaaa agtggcatga
     8641 ccatggataa tccagagatc tccgtcgtac cagttccaga gactgaaata aagaaaatca
     8701 cattgaagaa cgactactta gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa
     8761 tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg
     8821 aagatgttct aacagacaaa agcttttttg gagcaactgt cggacctgta gccggaagga
     8881 tccgggatgc ttcttggggt gagcatagat tggagaaaaa ctgcggatca caccatatcc
     8941 acggtggcac gaaaggctgg tctttccaat tttgggaagt agaagttttc aaaactccat
     9001 catcaatagg tgtggtcttt tacttaaaag atgaattttc tacctatcca ggaccaatca
     9061 ctgctacgat cacttaccgt ttaacaaaaa acgaattaga gatgacaacc tctgctagta
     9121 gccttgttga aaccatctgc aatccgacca accatgccta cttcaacctt tcaggaaatg
     9181 gaacaagaga catctatgaa catcagttaa cggtcttttt agatggtgtg cttgaattag
     9241 atcaagaaaa actgcctact ggaaattgga caaaaaaaga aaaactgcca atcgacttta
     9301 gaaaatcccc aacactgcaa gagatccttg catgttatcc agatggacta gatgatgtat
     9361 ttcttttgca tcatcctagg ctttccaaaa cttccttaca attattcgag aagcattctg
     9421 gtcgccaaat gactatagca acttctaata aaagtatggt attattttct acgactggat
     9481 tcgaagctga tttctctgtc aatggaaaac agatgcattc aaactacgga ttagcaatcg
     9541 aaccgcaaga aatccctgat atcgttcatt tccccaaatt tggatcgatc aacctccatc
     9601 ctggtcaaga acggatcagt cagacgattt atcggttctc tgcacaataa caagagaaag
     9661 gtgtttttat catggctatt accgtaaaag atgtggcgaa aaaagccggt gttgccacct
     9721 cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca aagaagaaag
     9781 tccgaaaaat tatggatgaa ctaggttatg ttcctaacat gactgcccgc aatctaggaa
     9841 aacgcatttc cagtgccatc ggcgtaatct taccccctct tgattcaaaa gagcggatcg
     9901 gtaacccatt ttatcttgag atcatggaag caatcaacga agaagcgcgg aattatgata
     9961 tgactacagc aatcgctact gctaaaaatt ttgaattgct gttagagaac gtccagcgta
    10021 tgcatttaca aaaacaagta gatggtttta ttctagtgta ttcagatagt caagatactg
    10081 tcattgatta tttataccaa aaccacatcc ctttcacttt gatcggacag ccttatcatc
    10141 atgagaacga aatcgtttat gtagataatg ataaccagct tctaggtaaa caagcaacag
    10201 aatttttgat ccagaatgga cataagaata tcctgtttgt gacaaacacg actcatgaaa
    10261 gtttgtattt cgaacgatat ttcggttatc aaaaagcaat gatgatggct ggtcttcctg
    10321 cgttatccag tgtagtacta gagcatccgg aggattatct gtcctttgag aaaacgttga
    10381 aagaaaacga agccacagca ttggtggtca ttgacgatat cttcgccctt cgcacgatgc
    10441 agttggcgca aatgtatggc tatgtcattc cagagacact ctccgtcatt agcttcaaca
    10501 actcgatctt ctcaacatta acacaccctt accttacaag tatcgatatc gatatctccg
    10561 aattaggacg tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg
    10621 gcgtacgttt ggtcatccct catcgcttga tcaaaagaga gacagtcgtt cctttagaaa
    10681 acccatacta aatagctgac tcactaaata cgctgaccat ttatcctatg gtcagctttt
    10741 tatgtaaaag aagatactac ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca
    10801 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
    10861 actccttcgg aaataagctg aaattcacaa aaatttaaaa agcaattttc ttgagtttct
    10921 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttccttcc ttcaattttt
    10981 tacacgtcga cgattcaata aaaaaccgcc taatattgta aagacaggtg caagaatcga
    11041 gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc atcgttccca taatgaatgt
    11101 gccgctgact ccccacggta cgagagaatt gacggtcgca cctgaatccg ctagtgttcg
    11161 tgtcaaatag gtttttgata agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg
    11221 aagaatgatg gacagatact gttctccaac gatcagattg attccgatac agctcaaggc
    11281 agtaaacccg actaaacgag aaggtcggtc catcttttct tttaattctt gaacgatggt
    11341 atcaacgatc agatatttga tcagtaaccc acctaatgcc aatgcaagta aaatcaaact
    11401 agcagaacct aacatactca tgatcccgcc tcgagaaagc agttcatcta gctttggatc
    11461 aggtgtattt gcgacataac cattcatcaa gatgtctgct gcttttccga ccgtcaaact
    11521 tttctcatgg ataaaagaaa gaacgagagc agctgtcgat cccgctagta atgtagggat
    11581 agccggaatt tttttgattg cccccccaaa taaaataact agtggaatca atgtccaagg
    11641 agagatccaa aagctcgctt tcatgttttg gatcatttca ttgatactag ctagatcggc
    11701 tgaatgactg ttatgtccta atatcgtata aaaaatcaac gaaaggacga atgctgggaa
    11761 atccgtataa cgcataccag cgatatgttc gaatagatta actcctccaa tacccgctgc
    11821 caagttggtc gtatctgata aaggcgagca gttattccct aaaaaggcac cggaaacgac
    11881 tgcgcctgta gtcattgcat cattgaatcc aagtaaatga ccgatcccca aaaaagcaat
    11941 tccaatcgtc gaaatcgttg taaatgaact gccgacagtc actccgacaa ttgcacaaac
    12001 aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat aaaccataat
    12061 cgtagggatg gttccagata aaatccatga agcaactaag acaccaatca aaagaaaaat
    12121 caggattgga atgatccctg gagtgattcc tttgatgatc ccgtcatgga tctcatccca
    12181 tgtgaacttt ttaaatctgc cataaaacaa caaacacatg aaagctgcaa ggatcgggat
    12241 atgtggcgag agctggaatc cgatgatcag tactcctagg agtgccaaca acacgatcaa
    12301 tacagcgata ctttctttta aagaaatatt tttattcatc gtcgatctcc tatttcaaat
    12361 gaaaaggccc tgccatgaaa aaactattca cttggcagga cctcctacta aatcagcgac
    12421 aaaacgtcgg ctgaaagact tattttttta tttttcttct aaactttttt ctacctcaat
    12481 cccccaagaa tctttcttga agtgattgat gatcaatgga ataacgaaca tgaccactgt
    12541 gatagctgct actaataatg catcactcaa tgtcgctgta cctaatgtag caaatacgac
    12601 aacgatgata gagaagatca gcatgcagct gactacccaa gctaatccgt ttccgttttt
    12661 accaattcgg taaggacgtt cgaattctgg atgtttctta cgcattttaa gcaatgaaac
    12721 agcaatcaaa atgtacacga tacaataaat aacagttgta gcatttgtaa gtgtcaagaa
    12781 tacagcactt acgtcatcca ttacaccata aagcaaagca aataatgaaa taacgattga
    12841 ttgtgttaga acgacatttc tagatacgcc gtatttgttt tctttatgga atccgaattt
    12901 tggtggaagg aatccttcac gagctacttg gatgattgtt tttgatggtc cagtaaccca
    12961 agctgataat tgtaaaagca caccaataaa taccatgaag ctgaagatat ttccgatcac
    13021 ttctggtaag cctaggattt gacagtcaat caagataggc tgcgtgatat ttgctaattc
    13081 catttttcca tctggtacga catttgctac aagcatcgca ttgatgacat tcaataaaac
    13141 taatccgatc aaagcgataa atacgccttt tgtataattt tttgttgcgt ctttcaaacg
    13201 aggcatgtaa acagaactca tttcgatccc aacaaaaata aatgtgatac cagctaagta
    13261 tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt gaccatgaaa agtcacctag
    13321 atagccgttt gagactagtc cgactttgaa ggctgccaaa acacctaaaa cgaacatgat
    13381 gactactggg atatataccc ctagccatac accgatgttc ccgccgactt ttgccatatc
    13441 gaatttcaag ttcaagatcg tgatgatcca gtagataact aaaatacaac ctaaaacgaa
    13501 ccagtgatta ttgccaagtt ctacattacc aaatgtgttt cctaacaatg gtcctaaagt
    13561 agaagcgacc attaccatac ctgggaacat ttgcacccat aatagccatg caacgacaaa
    13621 tccccatttg ctgcccagtg ctgtttttac ccataactga ggaccgcctt cttgaggaag
    13681 catcgtggaa agctcaccgg aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa
    13741 aattgaataa aaaatcaatg tccaaccgac tgcggctaac gtaggaatac tccgaaccgt
    13801 tgcacacagt gccattgtca gaccgataaa ggtcccaaga cccattttac ttacttcaga
    13861 tttactcacc gttgacactc tccttgattt tttggataaa ataacggtgt ctttgaagta
    13921 gtttcagcaa taaacgaaac aattcagcga atatttgagg ggattctttg agtttattgc
    13981 ttgaagggtt tcttcaaaga caacctcaaa acaaagggat cgaagagctt aggtctcccc
    14041 ttgctcttca gatccaaaca tgcgtcagaa acagttagta ttcaagctgt tttctaacat
    14101 gattaccttg cttattttgc ttcgcttgcc aataattggt ctgcgtagat tttttccaat
    14161 ttttcttgaa gagctgcttt gattttttca gcatattctt caaagtgttc ttctttgttc
    14221 atgtatggtg tcattacgct ggcacgcaat acagttactt ttccggcacg gttccattct
    14281 tcatctgaga atcctagttg gttcacaaat tgtaatgggc tgttgccata gtctgggata
    14341 gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa tgcttccttt cacgtatgaa
    14401 gaataatcat atacgtcatg gttcaattta ttcattgcaa ctaagtcatc gttgcctttt
    14461 tctttgaata catagtctac catattgaaa tctggataag ttaatggatg aacttcgatt
    14521 tctttgtctc ctactttaaa tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct
    14581 tcgattgatg cacccattaa tttaccatat cctgtgacat tcaaaggtaa gacatggtgt
    14641 gctgcccaaa cgctggctgc tgttgcccct gcttttgaac cttccaagat ataagcccca
    14701 agtaatgctg ggatatctgc gcctttttca aatacatatg tcgcaaagta agagataacg
    14761 tctctcatgc ggatatcttt gatgacgata ccaccggcag aatatggaac atagcccatt
    14821 ttatgtgggt caatcgttac agattctgct tcttcgattg ctttatatgc actgtgtact
    14881 tcttctaaga tataatcttt gttttctgta aagacattgt atttgtaatg aacatctttt
    14941 aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg
    15001 ccataagcag catctacgtg taagtagaag taaataccgt ctttttctaa tacgcgacgc
    15061 aatgcaacga ttttatcgat accgtcgatt gctccttctt ctgttgatcc tacaacaccg
    15121 actacaccta ggattggtgt tttttcagct gctagaccac gaacgatttt ttcaagttcg
    15181 ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa tcacttggtc taaaccgata
    15241 ccgatgatgt cggcagcttt tagccatgaa tagtgtttag tttgtggaac caaccatttt
    15301 cctaattttt ctaaatgttt tccgctacgt gctgagtgag ctttgatttc gtcgattttt
    15361 tctgggacgc tgtctaacag attcatgatt tcttctgttg acaagttcat taattcccaa
    15421 tcgctttttc cagcaactag ttcaggtgtc acttctttca ttgcaagcgg caatgatttg
    15481 atgttgcggg cataccaaag tccttctaag tttgctaaag aaccatcagc aacgatgtgg
    15541 ccccagccat ctttatagct cattaatttt gcaaattcca ttcctacttc ttcttccatt
    15601 tggcttgttg caggagaaga ttcataggca acgttattac cattccatag catagcaaaa
    15661 ttgtacgcta gtaaagatgg catcaatgtt tcagagttca tatgacccca gtaacgtccg
    15721 gcattatgcc aaggaacgga atgggtacgc atccgcgctg aaatttctga tagaacatcc
    15781 tttgttttat tcacagtgtg ttcaaaacta gcactgcttt tttcttctgg cgtaataata
    15841 ggcatgtctt gaggcatgta gttttgacgc cagcctaaat gttcatccac caattcattc
    15901 aacaaagctt tatagatttg gccgttctca gctttgtccc caatgaatag ggcatttagg
    15961 tttagatctt tcgacaatga ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt
    16021 ttgtcgagcc ctacattaca cctgttcttc tattctgtaa actaagtttt ggcctgattt
    16081 ttaatcatat tttaatatgt atcaattatc cgaattgttc acacttttga aataattaaa
    16141 aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag attgtattaa cgacaaccag
    16201 cgttctattt taggatgggt ttcaaaattg gaatgttgta atagtatttc cctttttcaa
    16261 ggtaaaaact tactaaattc taatcaatta ctttggccaa aaagtaattt ttcttacctt
    16321 ttcgaataac cacaaactta ccatcgaatt catcggatgg atttatgaca aaatttaaat
    16381 cggtaacgcg gtcaccgtta atacttatag ctccgtttga aacatcttca cgtgcttgac
    16441 gtttagatgg ttcaattttt gtggaaacaa gcagttccac gatattttca ggtgtgcttg
    16501 aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca tttagttctt
    16561 taatatttcc agagaacaat gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac
    16621 tatgcacaaa tcttgtaact tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt
    16681 ctgtctctac ttttttcgct aagtcttcga tttcttcttg agacaagaaa gtaaagaatt
    16741 tcaagtattt cactacatcg cgatcatctt gattcaacca gaattggtag aactcaaatg
    16801 gtgaagtttt cttcggatca agccaaacgg caccgcctgc agtttttccg aatttcgttc
    16861 cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc ttctgcttct ggtccttcta
    16921 attttcggat taggtctaat ccagctgtga tatttcccca ttggtctgct ccaccgattt
    16981 gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc aggatttggt
    17041 aagtaaactc tgtaaatgag attcctactt ccaaacggct ggcaacgata tcttttgcta
    17101 acatcgtgtt gatgttgaag tttttaccgt agtctctcaa aaattcaagt aaagagattt
    17161 ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc atctttacca aacaattttc
    17221 tcatttgatt ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt tgcaacacac
    17281 gttctgtttt gcggccgctt gggtcgccga tcgttcctgt tccgccgcca atcagaatat
    17341 atggatgatg gcctgccaat tggaatcttt tcatcatcat aaatggaatc aaatgtccga
    17401 tatgcatcga atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa
    17461 cgagctcgcg aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt
    17521 catcaataat attcatttta ctgacctccg tgtagttttt ttatgaaagc aggaacctgc
    17581 tagcatatgt aattaattat ttcttcagga taggatagga aataaaaaaa gtcccttggc
    17641 atataagcca agggacgaaa tctcgcggta ccacccaatt tgtatgtcta gatatccaac
    17701 atacctctcg aacatttaac gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt
    17761 aattcgcttt aaagcatgta ctggtttgca gcgaccacca gctttctgaa acattggctt
    17821 taacaactac ttcgtttcga tcttcgtctt gttattatta atatgtgtct atcatagagc
    17881 gcaacttttt ctttgtcaac agaaaaagga tatttttttt aaattcctca ttttttcttt
    17941 tttgaatccg ctctaattcc gaacatttta ttttcaatta ttgttaaaag aaataacttt
    18001 ttctgtattt tttctgcaat ttatcgagaa gtcactttac tttgcctctt attctttctt
    18061 cgttacaata aggaatggga caagtaaaaa aatgaaaaag caagggacga ggtatatgga
    18121 aaaaaagcaa aaaattattt taggagccac tt
//
LOCUS       DASJON010000040        18092 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJON010000040 DASJON010000000
VERSION     DASJON010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.040"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..2295
                     /locus_tag="U3989_001011"
     CDS             <1..2295
                     /locus_tag="U3989_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN1714473.1"
                     /translation="LFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQGTYWTR
                     EKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEAANVFY
                     QFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESAFDWSL
                     FQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDRKETKG
                     LLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQSYDTDG
                     SLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLALDEKKP
                     VPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTSISRME
                     NFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLVNMSDA
                     QRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNTGMHPL
                     QTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDYKSSGR
                     SFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTETDDVEK
                     NTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKDKFFTE
                     EELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDVMLKEN
                     NYHRLEKIDKEEALRRILKRSEEE"
     gene            2292..6014
                     /gene="addA"
                     /locus_tag="U3989_001012"
     CDS             2292..6014
                     /gene="addA"
                     /locus_tag="U3989_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN1714474.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            6083..6424
                     /locus_tag="U3989_001013"
     CDS             6083..6424
                     /locus_tag="U3989_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1714475.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            6733..7776
                     /gene="pheS"
                     /locus_tag="U3989_001014"
     CDS             6733..7776
                     /gene="pheS"
                     /locus_tag="U3989_001014"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN1714476.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            7781..10201
                     /locus_tag="U3989_001015"
     CDS             7781..10201
                     /locus_tag="U3989_001015"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN1714477.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(10371..11381)
                     /gene="trpS"
                     /locus_tag="U3989_001016"
     CDS             complement(10371..11381)
                     /gene="trpS"
                     /locus_tag="U3989_001016"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN1714478.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            11635..12372
                     /locus_tag="U3989_001017"
     CDS             11635..12372
                     /locus_tag="U3989_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1714479.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            12386..13201
                     /locus_tag="U3989_001018"
     CDS             12386..13201
                     /locus_tag="U3989_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1714480.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            13214..13861
                     /locus_tag="U3989_001019"
     CDS             13214..13861
                     /locus_tag="U3989_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1714481.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            13877..14533
                     /locus_tag="U3989_001020"
     CDS             13877..14533
                     /locus_tag="U3989_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1714482.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            14695..15516
                     /gene="racE"
                     /locus_tag="U3989_001021"
     CDS             14695..15516
                     /gene="racE"
                     /locus_tag="U3989_001021"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN1714483.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            15519..16874
                     /gene="rph"
                     /locus_tag="U3989_001022"
     CDS             15519..16874
                     /gene="rph"
                     /locus_tag="U3989_001022"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN1714484.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            16875..17393
                     /locus_tag="U3989_001023"
     CDS             16875..17393
                     /locus_tag="U3989_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1714485.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            17500..18000
                     /locus_tag="U3989_001024"
     CDS             17500..18000
                     /locus_tag="U3989_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1714486.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6069 a         3098 c         3903 g         5022 t
ORIGIN      
        1 ttatttctgt cgacggaagc agaaaccgaa cttgatgaat ggaaacaaga gcgagatgaa
       61 ttcaggaaaa aactggacat cactgaaaat gtagcacttg cttacaatta tcaaggaact
      121 tattggactc gtgaaaaaga ctggcagttt gtggcgtatg attttgaagc agaacagatt
      181 gaagacgtac agtatttgga agaacagtca aatacgatta gacgattatt tcagaaagct
      241 gtaccagtat ttttgaaaaa aataaagaaa agcaaaacag gacaagaggc cgcaaatgtt
      301 ttttatcaat ttttgatcca aagtggcgta cagcaacaat tattgttttg gcgtaatcag
      361 gaagtagaca gaggaaactt agaagcggca agaaaccatg agcagacatg gggagcgctg
      421 attgatctgt tggatgaata tgtagtaatt tatggtgaat cggcttttga ctggtcactg
      481 ttccaagaaa tcattacaag tggtctagaa aatctaagtt atggtaaaat ccctaccgca
      541 atcgaccaag tgcgaatcaa tcgtttggaa ttagttcgac caaatcaagc tgccgtcact
      601 tttgcattag ggttgaatga tcaagtgttt ccagaccgaa aagagacaaa aggattgttg
      661 tccagtgaag aacgagaagt attaaatcag gccttgccag atggacagtt tttattcgac
      721 ccatcaaagg aaagtatctc ttttgagcct ttccaagcct atcttgtttt tctttccgga
      781 acagatcgat tatatcttag ttatgcacaa agctatgaca cagatggttc gctgaagatg
      841 tctccttatt tgcgtagaat cacagaatat ttatctgttc ccgtcgagag aaaagagcat
      901 ttgacgatcg aaagcgaccc taattgttat gtagggactt acagaagcgg aatcaataca
      961 gtcaatagga tttatcgatt agctttagat gaaaaaaaac ctgtaccaaa gcactggcaa
     1021 aagctcaaag aattagtact gaattcgtca tggcgaaatt ttgctttaca gatttttgaa
     1081 agtcaggaac atttaaatgt tcctgtttcg atgccagaaa aaatggcgga agagctttat
     1141 ggaaaagaga tttatacttc aatttcacgt atggagaatt tttataattg tgaatacaaa
     1201 tacttcatgc aatttggttt gaaattgaaa gaacgaactg tttatggtct aactccagca
     1261 gcaactggtg atttttatca tgaatcattg gatcgtttct tcaagctact gttttcaaac
     1321 caattatcct tagtgaatat gtccgatgcg cagcgtagag actttactga aaaagtgctg
     1381 caggaagtat tcggagaact tcggtttgag atattggata gttctgcaag aatgaactat
     1441 atccgctacc agttaggaca gacgattcaa aaagtgacat gggctttgca aaaacagagc
     1501 aaaaatacag gcatgcatcc tcttcagaca gagattctat ttggtcagat tgctggagca
     1561 aaaggtatca caggtctgga attaccgttg agtaatggtg gaaaactcca tgttcgaggg
     1621 aaaatcgatc gaatcgatgt agcttctgaa caagaagata cttggctaag cgtcgttgat
     1681 tacaaatcta gcggacgttc attcgatgtc acagaagctt attatggcat ggctatgcaa
     1741 ttattgacgt atctggacgt agcattgatg gatgcggtac gtttgactgg aaaggcggct
     1801 gttaaaccag caggatcata ttatttacat gttcataatc ctgtattgac ggagacagat
     1861 gatgtagaga agaatacatt gaaaaaattt agttatgatg gcgtttttgt cgatgatccc
     1921 gctcttttag aagtactaga tcattcactg gaagcaaaag aacaatcact gatctatcca
     1981 gtgaaaaaaa atgcgaaaga tgaataccaa aaaggtagtt tcagtaaaga caaatttttc
     2041 acggaagaag aactgcaact attcatggcg cataatcggg aaaatatgcg gttagctgga
     2101 gatgcgatta cttccggaga aatcaaacta aatccagctt ataaagataa acagcggatt
     2161 gcctgcgaat tttgtccatt cagaagcatc tgtacgtttg atgtgatgct gaaagaaaat
     2221 aattaccacc gtctggagaa aatagataag gaagaagcac tgcgtagaat attgaaaagg
     2281 agtgaagaag agtgaatcaa attcctttaa aaccgcaaaa tgaacgattc acagatgatc
     2341 agtggcaagc gatttttgat caaggagata atctattagt ctctgcgtct gccggatcag
     2401 gtaaaactac agtgcttgtt cgaagagtga ttgaaaaact aaaaatgggt tttgatatcg
     2461 atgaattatt gattgtgaca tttactgaag ctgccgcaag agaaatgaaa gaaagaattc
     2521 aagaggcatt acaagaatcc gtcaatagcg aaagtgaccc tgtacgtaga cagcatttta
     2581 ctaagcagtt agttttattg cccacagcga atatctcaac cttacatgct ttttgcctga
     2641 cagttattcg acgttattat tacttaattg atattgatcc agtctttcgc atgttaacag
     2701 atgaaacaga aacgatcttg atgaaggagg atgtgtggga cgagctgaga gaagcacttt
     2761 atgcagaaaa tgatgaacga tttttccaat taacaatgaa tttttctaat gatcgttcag
     2821 atgacggctt gacgaatctg gtgttttctc tttatgagtt tgcccgtgcc aaccctgacc
     2881 cccaaaaatg gctagaacag ttaagcgata attatcgttt gcctgagggg ctagcgaaat
     2941 ctcgattata tcaaaaacaa atccgtcctt tagttttggc agatatatac caatgtgtac
     3001 agttatatga acagatgact cagttagcac aaggtgaagg attagaaaaa atgcatgagc
     3061 aagtagctgg agaacaacag cagatcaaga atatctatga agcgttctcg caagatcggt
     3121 tagaagaagc ttatgctggt ttggaacaac taactttttc aacttttaag agcagtcgga
     3181 aagcagaatt gaaagaaatt tccaatgaag tcaagggcat gagggataaa gcaaaaaaac
     3241 tgattcagca gataagtaaa agttattttc ctgtttcccc aagccaaatg gaagaattaa
     3301 cggacaaagc actaccgtta gtcgaagaaa tgacaaaagt cacgcaaagc ttcatggacg
     3361 gtttcagtat gcgtaaacgg gaaaaaggtg tgctggattt caatgatctg gaacatttag
     3421 cgttgcagat tctcacagaa aaaacgaaag atgtatggct gccaagtgaa gcttcaaaac
     3481 attatcgaaa aaaattcaaa gaagtaatgg tagatgaata tcaagacgtg aaccaattac
     3541 aagaagccat tttatactgg ctaagagaac ccgatgatac aaaaggaaat atgttcatgg
     3601 ttggggatgt caaacaatcc atctattctt tccgtttagc agatcctagt ttatttatcg
     3661 ggaaatacga gaatttctca aagaaagaag gcggacgtcg aatcgttttg gctgagaact
     3721 tccgctctag aaaagaagtc ctttctttta cgaatcttat ttttgaacaa ctaatggatc
     3781 cagcagtggg tcaaatcaat tatgacgaag cagccaaact gatccaagga ttttcagact
     3841 ttccagagaa tgaacagttt gagccggaaa tcatgatcta tgaaaaagaa caggaagaat
     3901 cagagattga gattccgacg gatgatatac tggaagacaa aacagaaggc gaacttttta
     3961 tgacaggact aaaaattcgt caattaattg attcctcctt tatgatttat gataaaaaat
     4021 caaaaaaaag tcgtccgata gagtacaaag acattgttct cttgacacct acaaagaaga
     4081 acaacttaac gattttagag attttcaaaa cattggatat tccattagag atgaatgatg
     4141 ctcaaaacta ttttcaagcg acagaaattc gcacgatgat ctcgctgcta caattaatcg
     4201 ataatcccta tcaagatatt ccgcttgcgg ctgtattgcg ctcaccgatc gtgggtctga
     4261 ttgaaccaga attagccagc attcgattag ctaaccgtgc acatacttac tatgatgctg
     4321 tgcttgctta tcaagcatca aacgaagatg aattagcagc taagctggaa cattttggca
     4381 agcaactaga gcattggcga gaacttgctc gccgttcttc gatcactgac ttgttatggg
     4441 atatctatta tgagacggga tatctagagt atgtggtcgg tctgccagca ggggcacaga
     4501 gacaagccaa tctttatgca ttggttgatc gtgccaaagc ttacgagcaa agttctttca
     4561 gaggattata ccagttcgtg cgtttcattg aaaaaatgca agaaaaagat aaagacttag
     4621 cagaaccagt catttcaata gaagataatg ctgttcgcgt tatgacgatc catgcaagta
     4681 aaggcttgga attcccagtt gtatttttgt tagatatgac aaaggagttc aacttacaag
     4741 acttgcgcaa tcggtatgct tttgaagaaa aacttggagc aggaattcgc tatatggatc
     4801 ctgaaacacg agtgttatat gatacgttac cgtttcaagc catcaaattg gcaaagcaaa
     4861 acaaattgtt gtcggaagaa atgcgtaaat tgtatgtagg attgactcgg gcagaacaaa
     4921 agcttttcat tgtcggttcc tataaaaata aagagcaaat gatccaaacg tggtctgaag
     4981 cagctgatca tgaggagtta gtttttgatc ctgcgctacg tttgaagggc agaagcagct
     5041 tgatgaattg gattggttac ggattgatcc gtcatcctga aatgcagaaa tacttagagg
     5101 aagaaatttc gacttctctt cttcaacatt caaatgcaca gttttctatt tcatggatga
     5161 atcagcaatc gatcattgaa caaagacagt tgcttgctga aaaagaactg gttaacttag
     5221 atcagcagat gaaagaagat gaaactttat tagcagattc gttacaaaaa cgtctggcat
     5281 atgaatatcc ttatcaagct tcttcacaaa ccacaagtta tcaatctgta tctgaaatca
     5341 aacgattatt cgaagatcca gatgatacac aagaatcacg tttgactctg gaaagcagtc
     5401 agaacaaagc agcatcccgt caatttcgtt atacgcaaga acaactagca gaaccaaaat
     5461 ttttgcaaaa agatcggcaa gtgtcagcag ctgctgttgg aactgctacg catgctttgt
     5521 tacaattact gccattagag atgccaacta cagagagtat ccatcaaaaa ttacaggaat
     5581 tggtaaggaa acggttagta gacgaaaaag tcgcgaaaaa agtagatgtt tcttctatta
     5641 tatggttctt tcaaacagaa cttggtcaac agctgattgc caacaaagaa aacgtaaaac
     5701 gagaacaacc tttttccatg ctgcttcctg ctgacgaagt atttcaagac tatccaaatc
     5761 aagaagatga actattaatc cacggaatcg tcgatggata cctagaagaa aaagatcatc
     5821 tcaatatcta tgattttaaa acagatttta ttttacatcc ggatgatcca gcagaaatag
     5881 atgcaatcgt ccaaaaatat caaggacagc taagacttta ccaacaagca atgtcagaag
     5941 ctttgaataa gccagtagaa aatgttttcc taattttgct tcgagtgaaa caaattataa
     6001 acattaacaa ataattataa ttgagcaaac ttgacttatg ttttgtaagg attatgctat
     6061 actctgggaa gaggtgagta tgatggagct aacacagaca gaattcgttt tatgccctaa
     6121 atttgaaaaa acattctcta ttttaggaaa aaaatggaac ggattgatta tcgatgtact
     6181 actcgaaaac ggtccgcaac gctttgttga gcttgcggca aaaatcccga tggtcagtga
     6241 tcgagtatta gtcgaacgct tgaaggaatt agaaaaagaa ggaatagtca ctcgaaactt
     6301 tgcctgtagc gaaaagagcc gttcagaata tctcttgact gaaaaaggag cagacttgca
     6361 aaaagcgatg cgtcaaatac aagtttggag cgaaaaatgg atcacagagg aagaatgcgg
     6421 ttgattttgt aaaaaaaatc ttgtaaacta tagtccatac gaataagtaa agaacgatga
     6481 cgaaaaagag tagtttggct aaaccagttc agggagatct gtcgcagact gaaagcagat
     6541 cctgtgtttg aaaaatgaag ttcactttcc gagttcgtct gataaaatca gaccggcaat
     6601 tgtcgttaac gaagtaagag tgcggtgtaa gtcatttatc cgcaaattag gatggtaacg
     6661 cgaagacctc gttccttaac aggaatgagg tctttttgtt ttgtatttgg aatttaaagg
     6721 aggaacaagc acatgtctct acaagcacaa ttagagagct tgaaagaagc aacgctcgtc
     6781 aaaatcacag cggcacaaga tttaaatgaa ttaaatcaaa tacgtgttga aacgttagga
     6841 aaaaaaggac cgatcacaga agtattacgt gggatgaaag atctaagtcc agaagagcga
     6901 ccaaaagtcg gaagtttcgc aaatgagatc cgagatcttt tgacagaaga gatcgagcga
     6961 aaaaaacaag tattagaaga agaagcgtta aatgcagctt tggcaaaaga aacaatagac
     7021 gtgacactac ctggtagaaa agtaaaacat ggtacacgtc atatcgtgac acaaatcatg
     7081 gaagaaattg aagatatttt tgtgggtatg ggctatcaga tcgtagaagg atatgaagta
     7141 gaatctgatc attacaattt cgaacggatg aatctgccaa aagatcatcc agcaagggac
     7201 atgcaagata ctttctatat ttcagacgag atattgattc ggacacatac ttcaccagtc
     7261 caagcacgga caatggaaaa acatgatttc tctaagggtg ctctacgaat gatctcgcca
     7321 gggaaagtct tccgcagaga tacagatgat gcgacccaca gtcatcagtt ccatcaaatt
     7381 gaagggctag ttgttgataa aaacatcaca atgggcgacc ttaaaggaac attagaagtc
     7441 gtaatgaaaa aaatgtttgg ggaagatcgt gaaattcgtt tgcgtccaag ttatttccca
     7501 tttacggaac catcagtaga agtagatgtc agctgtttca aatgtggtgg tgccggttgt
     7561 aacgtatgta aatacactgg atggatcgaa attttaggag ctggcatggt acacccgaat
     7621 gtgttgaaga tgtcaggaat cgatccagaa gaatattcag gctttgcttt cggccttgga
     7681 ccagaccgcg tagctatgtt gcggtatgga gtgaatgata tccgtaattt ctatcaaaat
     7741 gacttacgtt tcttaagtca gttcaagggg gagtaagaaa atgttagttt catataaatg
     7801 gctcaatcgt tatgtagatt tatcgaatgt aacaccaaaa gaattagcgg ataagatgtc
     7861 tgtgacagga atcgaagtag agggagtaac ggttccagaa gaaggcttga aaaaaatcgt
     7921 tgtcggtgaa gtcaaggaat gcgtaccaca tccagattct gatcaccttt ctatttgtca
     7981 agtcgatatt ggcgaagacg aactgtctca aatcgtttgt ggagcgccta atgtgaaggt
     8041 tggaatcaaa gtgattgttg cacttcctaa ctcacgtatc gctggtaatg taaaaatcaa
     8101 aaaaggaaaa atgcgtggcc aagtatcaaa tggcatgatt tgctcattac aagaaattgg
     8161 ctattcagat agtgttatac cgaaagaata cgctgaaggt atctattata tgccacaaga
     8221 agctgtaaat ggcgatccag tttttcctta tttggatatg gatgattcga ttattgaatt
     8281 atctatcaca ccgaatcgtg cggatgcatt gtcaatgcgt ggcgtagctt atgaagttgg
     8341 ggcaatttat cgtcagactc ctacattcga tgacgaaaag ctggtagaat cctcacgtcc
     8401 tgctagcgat aaaattcaag tggaagttga agataaagaa gcagttcctg cttatcaaat
     8461 cagaatcatt gaaggagtaa gaatccagcc aagtcctcaa tggctgcaaa atcttttgat
     8521 gaacgaaggc attcgtccaa tcaacaatgt agtagatgtg acgaactata ttttgctttt
     8581 attcggacaa ccactacatg catttgacta cgacaaattg aaatcaaaag agatctttgt
     8641 ccgccgtgca aaagaaaatg aaacattagt aacactagat ggagaatctc gggaattgac
     8701 aactgacaat ctcgtcatta ctaacggcaa agaaccagtt gctttagctg gtgtcatggg
     8761 agggttagac tcagagatca cgaatgaaac aacaacagtt gcactagaat ctgctttatt
     8821 tgatccaatt tcgattcgtc gtacagcaaa acaatttaat ttgcgtagtg aatcctctgc
     8881 acgttttgaa aaaggaatca accatgcgac tgttggagaa gccagtgagg ttgctgcagc
     8941 aatgatcgtt tctcttgcgg gtggggaagt tctacaaggc gcggtcaaag gctcagagat
     9001 caaagcagaa gacgtatcag tgaagatttc acttgaacgc atcaatcaat atttaggaac
     9061 atctcttgtc gttaaagaag taaatgagat ttttgaagca cttggattta gctatgaaga
     9121 aaaggagtat gtttattccg tagtagtacc accaagacgc tgggatatcc aaatcgaagc
     9181 agatattatc gaagaagttg ctcgtattta tggatacgac cagttgccat ctacacttcc
     9241 aagtggagaa acggttgctg gaagcttgtc aaaagaacaa gaaactactc gtaaaattcg
     9301 tacaatcttt gaaggaagcg gattgagcga agcaatcagt tatgcattga caacagaaga
     9361 aaaatcaaga cagtttgtta taaatgaaag taatattaca cgtttggact ggccaatgag
     9421 cgaagaacgt tctgtattac gaatgaattt gatcagcgga ctattagatg atatcagtta
     9481 taacactgcc cgaaaaaata cagagattgc tttatatgaa attggtcggg tgttcttcca
     9541 agaaaatgat ccacgtacac atctgccgca agaagtaaaa catgcagcaa tcgctatttc
     9601 tggtatttgg gaagaaaaag attggcagac gaaagctcaa aatgtagact ttttcaccat
     9661 caagggtctt ttagaaaact tatttgaaca actaggcatc actgaagatg tccattatca
     9721 aacagtgaca gaaatgaagg aaatgcatcc tggaagaact gctgcgattt accttggcga
     9781 aaaatttatt ggttttgtcg gtcaagtgca tccaacgatt acaaaaagtt atgatattcc
     9841 agaaacatat gttgctgagt ttgatttatc tgctatagtg gaagctgctc aaaaagggat
     9901 cgttttcgaa gcagtgacaa aattcccagc agtcagccgg gacatcgctt tgcttgtaaa
     9961 agaaactgtg acaaatcagg aattaacaga agttatccgt tctgctgccg gaagattttt
    10021 aacagatatc caattatttg atgtctatca aggagaaaac attgaaaaag gacataaatc
    10081 catggcgtat agcctgacat tcgtcaaccc agaagcaaca ttgacagatg aagaaatcaa
    10141 taaagcaatg gaaaaagtcg aaaaagcgtt ggtagaaaac ctagaggcaa gtattcgcta
    10201 agaaggaaaa tatagtaagt aacgatcaag tagcttttga agaccataca tttgaaaaaa
    10261 agatgtgaca caacttcgtc acatctcttt ttttgatttt aaaaaaggaa tagcacagag
    10321 acgagataaa aaaacaggcc tttacagcct gcttttttag agtaaatgaa ttaatggaag
    10381 taattgattc ccattgctga ttttacttca gaaagtgttt gtgcagctac tttagcagct
    10441 tcttcactac ctttacgtaa gatttccata acagctgaag gatctttttc caattcttct
    10501 cggcgcgcac ggatcggtgc aaattcagct tccaatacat caatgaggta tcgtttgatc
    10561 tttacatcac caagtccgcc gcggcggtaa tgagctttca tttcttcaat tgcttcttta
    10621 tctgttccga atacatccaa gtaggtgaag accatattcc cttctacttg tccaggatct
    10681 tgtacatgga tgtggtttgg atcagtatac atactcatga cttttttctg taagacatcg
    10741 gcaggatcgg agatataaat accgtttcct aacgatttac tcattttgcc atttccatcg
    10801 attccaggta atctacccat tccttttggt gggaatacgc cttttggttc caccaagact
    10861 tcaccatatg tatgattgaa gctttgtacg atttcctgtg tctgctctag cattggtttt
    10921 tgatcctcac caacaggtac aagattcgct ttgaatgcag tgatatctgc tgcttgagaa
    10981 acaggataga taaagaatcc tgtaggcacg ctttcaccga attttttttg ttcgatttct
    11041 gttttgacag tcggattgcg ccgaacgcgt cctacgctga ctaagttcaa ataatacatg
    11101 gttaattcag ctaattccgg aatttgtgat tggataaata aagtagattt tgcaggatct
    11161 aatcccacgg ctaaatagtc taaagcaact tgcaagacat ttgatgaaac cttctcagga
    11221 tttttagcat tatctgtcag tgcttgcatg tcagcaatca tgacaaacag ttggttgtct
    11281 tcatctgcct gcatagccac tcgggttttt agagaaccga cgtaatgccc taaatgcagc
    11341 ttcccagttg gtcgatctcc cgttaaaata atatttttca aaaaagacac ctctttacga
    11401 tttgtaagtc tgagcactta atatcttacc gtttttcctg aaaagaaaca acagttccta
    11461 aggaaaaaac aaggaaagag gataaaataa ctaatagatt agaaatggaa gaaatattta
    11521 tgggagaata aacgttctaa gaaactttac aaattactaa actatcagat ataatgtatt
    11581 ttattataca tctttaaaag aaatcttact aaataataat gggagtgtaa ttctatggca
    11641 atgattaaat ttgatcacgt ggataaatat tatggcaagt tccacgcatt aaaaaatatc
    11701 aatctcgaat tcgagaaagg agaagttgtc gttgtcattg gtccttctgg ttcaggaaag
    11761 agcacgatgc ttcgctgtat caatggatta gagacgatct cttcaggtaa gctgctgatt
    11821 aatgatactg acttgcatga taaaaagaca aaactgacag aagtccgaaa aaacatcggc
    11881 atggttttcc aacatttcaa tttgtaccca aataaaactg ttctggaaaa catcaaatta
    11941 gcaccaatca aagtgttgaa gcaagaccag gcaactgcag tcaaaaacgc ggagaaattt
    12001 ttgaaaactg tcaatatgtt agataagaaa gattcttatc cttccatgtt atccggggga
    12061 caacagcaac gggtggcaat cgccagaggt ttagcgatga atcctgagat gctgctgttt
    12121 gatgaaccta cttctgcttt ggatccggaa atgattggtg atgtattaga cgttatgaaa
    12181 aaattggctc atgacggtat gtcaatgatt gtcgtgacac atgaaatggg ctttgccaaa
    12241 gaagtggcgg atcgtgttat cttcatggct gatggagaag tattggaaga cagcagaaat
    12301 gtgagagact ttttcgaaaa tccaaatgaa gtccgagcac agcaatttat cagtaaagtt
    12361 atcaatcatt gataggagga aaaagatgcg caggttaaaa aaaatacgcc tgattttcct
    12421 tttaggcatt gcttttcttg cactagccgg atgtaaagga aatgggttgg caaatgaaga
    12481 tattttagag cgaagcaaat ctaccaatga aatcatctgg ggtgtcaagt atgatactcg
    12541 attatttgga atgatggata ttgagagcag aactgtacaa ggatttgatg tagatattgc
    12601 aaaagcaatc actaaaaaga tccttggtga caatggaaaa acagaatttg ttgaagtcac
    12661 ttctaaaact cggattccat tattgaaaaa cggaaatatc gatgcaatta ttgcaacgat
    12721 gacgatcaca gatgaacgaa aaaaacaggt tgacttttca gatgtctatt tcgatgctgg
    12781 ccaagctttg ttggtaaaga aaggaagcca aatcaaaagt gtagatgatt tgaatgcttc
    12841 tacaacggtt ctagcagtca aaggttctac atcggctgcg aatatccgac agcatgcacc
    12901 agatgcaaaa attttagaat tagaaaacta tgcagaagca tttactgctt tacaatctgg
    12961 tcagggagac gcgatgacta cggataatgc gattttgtta gggattgcag atgaaaatcc
    13021 agagtatgag ttagttggtg gaacgtttac taatgagcct tacgggattg cgatcaacaa
    13081 agggcaagaa aacttcttga aagctgttaa ccaagcgcta gaggaaatgc atgcagatgg
    13141 cacatacgat aaaatctatc aaaaatggtt tccaaatgaa actgaaggaa aagtagagta
    13201 agggggagag gcaatgagcg aattgataca aacttatgga ccaatctttg tcgacgggtt
    13261 caagatcaca ttgttttcga gtgtcttagc acttgtgttt agtttgatta tcggaacgtt
    13321 aatggcaatc ttccaattat caaagaataa agtagtgagc gccttagcaa aggcatatgt
    13381 agaatttttt agaaacattc cattattaat catcgtgatg tttttttatg ttgtcgtacc
    13441 gatttattgg tttaatctag atggattcca agcaggtacg attggattaa cgatctatac
    13501 atctgctttt attgctgaaa cagtacgttc agggattcaa accgtaccaa aaggtcaaac
    13561 agaagcagga ctatcttctg gttttaccta ttcagaaatc atgcgttata ttgttttacc
    13621 gcaagcattc aaaatcgtta tcccgccatt aggcaaccaa tttatcaacc tcgtaaaaaa
    13681 ctcatctatt ttagcaatcg tagctggttt agatttgatg tatcaaggag acttgatcgc
    13741 tagtgaaaca ttcaatacct tcgatacgta tatcatcgtt gggttatttt acttagttat
    13801 cacgctaccg ttatcttact taatggttta tcttgagaaa aaatgggcgg ttcgttcata
    13861 gaaggagggg aaatacatgg attttagcgg cgctttttca tggatgaata tccgtttttt
    13921 attggaagga ttaaaggtta ctgttgaagt ttcattgttt tcgatcattt ttagtttctt
    13981 gatcggtggt ctgacaggtg tgattcgttt tgcgaatatc cctttctttt ccaaaatcct
    14041 tggtgtagtg attgatatta tacggaatct tccattatta ttgatcattt tcttcactta
    14101 ctttgcctta cctcaaatcg gcattcagat gaacatcttc tggtccgctg tcgcagcatt
    14161 aacgattttt gagtcagcta tgttgtcgga aattttccga gctggactca atgctgttcc
    14221 aaaaggacag atggaagcag gattatcaac gggactgact tatgtagaat cgatgcgaac
    14281 gatcgtgtta ccgcaagcat ttaaatcaat gatcccggca attgtgagcc aattgatttc
    14341 actgatcaaa gatacttcat tagcagtaat catttcttta cctgaacttt ctcatcaggc
    14401 acgtattatt tatggacaga acaccaatta tgtgcttccg atgttcatca tcatgacact
    14461 tatgtacttc gtggtatgtt acgcgctttc tctgctttcc ggttacttgg aaaagagacg
    14521 gtacagttat taatagatga ttcttttagc ggttgtggca aactaaagtc acaaccgctt
    14581 ttcctctttc cattcgaaaa aaagagattt cgattgtttt aagcaagtaa gagcacttga
    14641 aaattcacgc taaaaagtac acaataggga ggatagaatt tattgaaaaa aaggatgata
    14701 cgattgacag ataatcgccc tatcggattt attgattcag gtgtcggcgg cttgactgta
    14761 gtaaaagaag ccctgaaaca attaccgaat gaaaatattt tatttgtagg agacacagca
    14821 cgctgcccat atggccctag acccgcggaa caggtaatcc agtatacttg ggaaatgacg
    14881 gattatctgg tggagcaagg aatcaagatg ctggtgatcg cctgcaatac cgcaactgcg
    14941 gtggctttag aagaaatcaa agctgctctt tctattccag tcatcggtgt gatccttccc
    15001 ggtactagag cggcagtaaa aaaaacacaa aataaacaag ttggcattat cggtacgatt
    15061 ggtacggtaa aaagtcaagc ttatgaaaaa gcactgaaag agaaagtacc agaattgact
    15121 gtgacaagtc ttgcttgtcc caaatttgtt tcagttgtcg aaagtaatga ataccattca
    15181 tcggtggcga aaaaaattgt ggcagaaaca ttagctcctt taaccactaa aaaaatcgat
    15241 acattgattt tgggatgcac ccattatcca ttattacgcc ccatcattca aaatgtaatg
    15301 ggagaaaatg ttcaactgat cgattctgga gcagaaacag taggtgaagt atctatgctg
    15361 ttagattatt tcaatttgag caattcaccg caaaatggtc ggacattatg ccagttttat
    15421 acaactggct ctgccaaact tttcgaggaa atagctgaag actggcttgg aatcggacac
    15481 ttaaatgtag aacatatcga attgggagga aaataaggat gcgtcatgac gggagaaaag
    15541 cccaagagct tcgcaaactg gagcttcaaa caaatgtttt caaatatcca gaaggatcgg
    15601 tagtgatccg ttttggtgat acaacagtta tttgttctgc aacaatagaa aattctgttc
    15661 cccctttttt gagagaaaca ggaacaggat gggtgacagc agaatatagt atgctgcctc
    15721 gagcaacgag tacgagaaat cgtagagaaa gcagcaaagg gaaactttct ggtagaacaa
    15781 tggaaatcca gcgtcttatt ggtcgttcct tgcgtgcagt agtagacttg gaaaaattag
    15841 gagaacgtag tatcatcgta gactgcgatg tgatccaagc ggatggcggt actagaacag
    15901 caagtatcac tggggcattt gtggcattga aattagccat tgaaaaactt ttaagagaaa
    15961 aagaattgtc agaagatcca ataaaagaac atcttgctgc agttagcgta ggaatcttac
    16021 cagatgggac ttgcgtcaca gatttggatt accaagaaga ttccgcagca cttgtagaca
    16081 tgaatcttgt aatgacagaa tctggaaaat tcgtggagat tcaaggaact ggagaagaag
    16141 caacgtttga tggtgaacag ttgaatgaaa tgctcttttt tggaaaaaac gcgattgaag
    16201 atttgatcaa agagcaaaaa catgctttgc taacagagtt tgctcaaaat gatgagcgga
    16261 tagaagaaac aaaaacaatc atcattgcga caagaaatcc aggaaaagca gaagagtttc
    16321 gaaatatgtt caaagaagcg ggatatcatg taaaaacatt acttgactat cctgaattgc
    16381 cagatgtgga agaaactggc agcacatttg aagaaaatgc ccgattaaaa gcagaaacga
    16441 ttgcacaatt gcttgatcag ccagtcttag cagatgattc aggtttgaaa gtggatgctt
    16501 taggcggtat gccaggaatc tattctgcac gttttgcagg ggaacaaaaa agtgatgcgg
    16561 ggaataacgc caaattatta tatgaattga cagatgtgcc ggatgaaaaa agaacagcac
    16621 aattccattg tacgttagta ttcgcggcac ccaaaaaaga cagccttgta gtagaagcgg
    16681 aatggcccgg tcgagtagcc cgtataccat ctggcgaaaa tggctttggg tatgatcctt
    16741 tatttatccc agaaggtaag aaacaaacag cagcagaatt atcgagcgaa gaaaaaaaca
    16801 agatcagtca tcgggctcaa gcaatgaaaa aattgagtgc cgagtggaaa caatggctgg
    16861 aaggagagag atagatgcgg tatttagtag taagtgataa tcatggagac agagatatcc
    16921 tcgttgactt agtagatcgt tatcgttctg aagtggatat ctttttccat tgtggtgatt
    16981 cggaattaga agcttctgac ccattatggg atacattcca agtggttcaa ggtaattgtg
    17041 attatggacc aggattcgaa cagaaaaaag tgatccaaac tggtcaggat acagtattca
    17101 tgactcacgg tcatttatcg aatgtccgat ttggtttgac acagttagca atcgaagctc
    17161 gagctgcaac tgccgatatg gtgttttttg gtcataccca tcaaataggc tgtgagatgg
    17221 atcagaaaat cctgtttttg aatcctggta gcatttctca gcctagaggc ccgatccaaa
    17281 tcccatcata tgctcttatc gaaagtttag atgaccaaat ttctgttcaa tactataacc
    17341 gtgcccatca ggcagttgaa gagttggcct tttctttttc gagagagcgc tgaataaaaa
    17401 aactctaatt tttaataaaa ataagcttta ttttatgaat tttgcatccg gtttcggtaa
    17461 aatagtagtg ttgaatgaaa tagaaatgga ggccatcaga tgattggacc tatagtaaga
    17521 gaaatgctgc tggaaaatca ggaaacgttt ttgattcctg cagaaaatgt ggcaaatgtt
    17581 atgtatcatc atccattgtc ccatggttta cttgtgttat cgaaggtcgg ttattccaaa
    17641 attcctgtat taggaaaaga tgatcacttt gtaggactag taagtttatc agatgtggtc
    17701 aataaaatga ttgacttgca aacaatcagc atggacccgt tagaaggact aacggttgct
    17761 gatgtgacag aaacagatgt tccgacaatt gatgaaaatt gggaactaga agaagtactc
    17821 catttgttag tcgattcgcc ttttttaccg gttgtcacag aagatcggat tttcaaagga
    17881 atcattacaa gaaaagagct gcttaaagcc gtaaacttca tggtgcatga attagaacgt
    17941 cgtaatatat tgtctccgaa aatcgaagaa ttaaaagaac aaatcgatat cgtgagctaa
    18001 agaacgagaa actaaaagat aatcaaaaaa taagtagaaa aaacagcgta ccagttttga
    18061 ctttatcttg aaaaagaggc tgggacaaaa gc
//
LOCUS       DASJON010000041        17860 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJON010000041 DASJON010000000
VERSION     DASJON010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.041"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..1241)
                     /locus_tag="U3989_001025"
     CDS             complement(39..1241)
                     /locus_tag="U3989_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1714487.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1358..1873
                     /locus_tag="U3989_001026"
     CDS             1358..1873
                     /locus_tag="U3989_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1714488.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2072..2518)
                     /locus_tag="U3989_001027"
     CDS             complement(2072..2518)
                     /locus_tag="U3989_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1714489.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2565..4043)
                     /gene="msr(C)"
                     /locus_tag="U3989_001028"
     CDS             complement(2565..4043)
                     /gene="msr(C)"
                     /locus_tag="U3989_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1714490.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4397..5026)
                     /locus_tag="U3989_001029"
     CDS             complement(4397..5026)
                     /locus_tag="U3989_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1714491.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(5010..5474)
                     /locus_tag="U3989_001030"
     CDS             complement(5010..5474)
                     /locus_tag="U3989_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1714492.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5961..7229)
                     /locus_tag="U3989_001031"
     CDS             complement(5961..7229)
                     /locus_tag="U3989_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1714493.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7460..8197)
                     /locus_tag="U3989_001032"
     CDS             complement(7460..8197)
                     /locus_tag="U3989_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1714494.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8264..8833)
                     /locus_tag="U3989_001033"
     CDS             complement(8264..8833)
                     /locus_tag="U3989_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN1714495.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8961..9833
                     /locus_tag="U3989_001034"
     CDS             8961..9833
                     /locus_tag="U3989_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1714496.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10175..11047
                     /locus_tag="U3989_001035"
     CDS             10175..11047
                     /locus_tag="U3989_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1714497.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11044..13002
                     /locus_tag="U3989_001036"
     CDS             11044..13002
                     /locus_tag="U3989_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1714498.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13334..14185)
                     /locus_tag="U3989_001037"
     CDS             complement(13334..14185)
                     /locus_tag="U3989_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1714499.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14308..14775
                     /locus_tag="U3989_001038"
     CDS             14308..14775
                     /locus_tag="U3989_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN1714500.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(15038..15520)
                     /locus_tag="U3989_001039"
     CDS             complement(15038..15520)
                     /locus_tag="U3989_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN1714501.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15620..16483)
                     /locus_tag="U3989_001040"
     CDS             complement(15620..16483)
                     /locus_tag="U3989_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1714502.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17081..>17860
                     /locus_tag="U3989_001041"
     CDS             17081..>17860
                     /locus_tag="U3989_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714503.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILK"
BASE COUNT         5381 a         3684 c         3163 g         5632 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc
     1021 atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta
     1081 gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa
     1141 gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt
     1201 gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca
     1261 tcatacatga tttattttac atgtcaatat gcacatgtaa aacgtggaaa ctttactgtg
     1321 atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt
     1381 attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat
     1441 tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg
     1501 ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga
     1561 tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa
     1621 agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg
     1681 actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt
     1741 gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag
     1801 aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca
     1861 gttttttgag taagggataa acagaacctt tctagaaata tctattgaag agcaaattat
     1921 cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac
     1981 ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg
     2041 ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca
     2101 cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca
     2161 ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt tccatgatca
     2221 caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga
     2281 tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca
     2341 caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca
     2401 acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga
     2461 ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc
     2521 tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta
     2581 agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca
     2641 aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct
     2701 aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta
     2761 cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta
     2821 cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca
     2881 agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc
     2941 atttgataac ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct
     3001 tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca
     3061 atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca
     3121 cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat
     3181 tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt
     3241 tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt
     3301 tgtttagagg aagacaaccg atccagtctg atttgttgct ttttcttttt actggacact
     3361 ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc
     3421 tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg
     3481 agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt
     3541 ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa
     3601 taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc
     3661 tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt
     3721 tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg
     3781 gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg
     3841 aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc tattagtttg
     3901 agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag
     3961 acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt
     4021 atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta
     4081 tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct
     4141 actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc acctattcta
     4201 ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt
     4261 gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt
     4321 ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat
     4381 agtaactgaa aaccttttat tcgaacatag tgatcttttc tggtgctaaa cgaacagact
     4441 cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt
     4501 tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt ggatttgttt
     4561 catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta gaatcgactt
     4621 tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta
     4681 attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg ttgtagattt
     4741 cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat
     4801 cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt
     4861 cagcgaccac aaagcgccat ggtttcatat tgacagaaga aggtgcagtc gtcgcttctt
     4921 tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg
     4981 atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct
     5041 ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat
     5101 tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc
     5161 atttcacaat gcttgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg
     5221 ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga
     5281 cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga
     5341 ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct
     5401 tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc
     5461 tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca
     5521 ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct
     5581 caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga
     5641 aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct
     5701 gcagtagata attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc
     5761 gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt
     5821 gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa
     5881 ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc
     5941 agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc
     6001 atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt
     6061 tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct
     6121 tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga
     6181 tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc
     6241 caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc
     6301 aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa
     6361 gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa
     6421 ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag
     6481 ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc
     6541 ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg
     6601 gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg
     6661 ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg
     6721 ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg
     6781 gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta
     6841 gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct
     6901 tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc
     6961 tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt
     7021 tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta
     7081 ctttctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt
     7141 tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata
     7201 acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc
     7261 ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt
     7321 tgtttctttt taataaattt gctttttctg cataaaaaga cgtaaatgaa gaatattctt
     7381 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctctct
     7441 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttgctga
     7501 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcagatg
     7561 ttaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
     7621 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
     7681 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
     7741 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
     7801 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
     7861 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
     7921 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
     7981 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
     8041 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
     8101 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
     8161 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
     8221 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
     8281 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
     8341 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
     8401 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc
     8461 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
     8521 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
     8581 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
     8641 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
     8701 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttccgct tggatctgat
     8761 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
     8821 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatgatg
     8881 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
     8941 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
     9001 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
     9061 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcga ggctcccgag
     9121 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
     9181 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
     9241 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
     9301 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
     9361 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
     9421 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cggaatcatc
     9481 ctttagcaaa gcaaacagtc atccatccgc aggaactgaa aactgttcct ttgatcattt
     9541 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
     9601 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
     9661 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaagtattca
     9721 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
     9781 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
     9841 ttttttcctt cgctttcagc tttttaacaa cgaataagtt aagcaaaaga tccaatgatt
     9901 aaataacata agtttcttaa tttgttcctt ccttttattt ctgataattc cagtaaatta
     9961 ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta
    10021 acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg
    10081 gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa
    10141 acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca
    10201 aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg
    10261 atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat
    10321 ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt
    10381 atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga
    10441 acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg
    10501 tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag
    10561 tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga
    10621 cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca
    10681 tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt
    10741 ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt
    10801 acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt
    10861 agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt
    10921 gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca
    10981 tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa
    11041 aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta
    11101 cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag taacgacatt
    11161 gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt
    11221 gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt
    11281 ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg
    11341 aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt
    11401 attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg
    11461 atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg
    11521 ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct
    11581 tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac
    11641 cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta
    11701 gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga
    11761 atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct
    11821 atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gttagcagca
    11881 gcttttgctt cattctttac tggtgtaaca gaaccacttg aattctcatt tatgttcgtt
    11941 gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca
    12001 ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct
    12061 ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca
    12121 gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc
    12181 cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat
    12241 gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc
    12301 gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat
    12361 aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc
    12421 atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct
    12481 gcaacacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtggaacg
    12541 atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca
    12601 gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga
    12661 gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc
    12721 ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac
    12781 ttaaaaggcg aaccattcac tttatatgta aaagaaggtc aaaaagtcgc aagaggtcaa
    12841 ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc
    12901 gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta
    12961 aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga
    13021 cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc
    13081 gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagccaa acgtaacata
    13141 tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc ttttttacaa
    13201 cctctgataa acggtgttca aaaaccagtt tatcggtatg aagtcaaatt tatgcaaaaa
    13261 tgtggaaagc cattttcaca aaatttttct tcatactcaa aactaatcgg ctttttcaca
    13321 acctctttat acattattcc tgatccaata tttcttttaa tgctgcttca agagaagtta
    13381 atggttttcc caatactttt tctaaatcat cagaggaaat agctaactga tcctttttaa
    13441 tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg agggaatccg gcttctttta
    13501 gattttcgac aaacgctgca tcacttgctt caataatttc taacttttta cctgtcgcac
    13561 gctctaatgc cttggctaat tcttcatagt cagttagatt gccggataat tctaagattt
    13621 ctggagagtc actttctact agtacttttg ctgcagcttc tgcatattca cgtttcaata
    13681 cccaacctgt ttttccattt cctgcagcat aaacgaattg accagttttt aaagcctgcc
    13741 caatgatagg taattcattc tctacatacc aattattccg taaaaatgta tgggaaatac
    13801 ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg cgctaataca ctgatcgagt
    13861 gatcggcatc tgcaaaactt gtataagcga tataagatac cccagctgct tgtgctgcct
    13921 tcactacatt cttatgttcg gcttgacgat ttcctggaac gccagagaca aaaagcaatc
    13981 gatctattcc tttcaacgct tctttcattg agtctaaatc atcataatca ccaatacgta
    14041 tattaaatcc agcttctttc agtgcttctc ctttttcttc actacgtact agtacataga
    14101 tttcattttg tggaaccatc ttttttaaga attccagcgc ataaccacca aaacctccag
    14161 ttgctcctgt tactaaataa tgcatatttg ccactcccta ttctttttat ttgttaagat
    14221 ttacagctgt acatttataa attacacctt tatcataaag agtgtggtaa gataagtcaa
    14281 gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc tgcaatttag tgatgcaatc
    14341 catgtacttg cctatattga aatgtttcaa gatacccgat cactttctag tgagatgatt
    14401 gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca gctcaaaaaa
    14461 tccggtttga tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa aagtccagat
    14521 cagatttctt tactagatgt ctatcgcagc atcgaaggta atacacagct tatccaagtc
    14581 gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc tttagaagaa
    14641 agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac acttgatagt
    14701 ctgatacata aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac aggcctcgta
    14761 gagagatttt tataattctc tctactgtgc ctgcattttt ctgatcggaa tcgtctacat
    14821 aaattctttt attctccggg ggacaaaagc cttgtcatgc ccctttttcc tctctaatgc
    14881 tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg tgttcaagta gctgaccctc
    14941 ggcattagtt ctgaaaaatg caaaatatgg aaagctattt cccatttttc ctctctaatg
    15001 ctcaggtcaa agcgctactt tcacgacctc tatattatca tggtttattg actaccggta
    15061 tccagatttc tcccatatat ttcccgttgg taactcccat ttcgactaca gcatttggac
    15121 ccccaacata ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc gtattcgtca
    15181 aatcatttct taattcttca ggcgattctg ctaaagcgat tacaacaaga tacgttcctt
    15241 ttgggaatgg gatgattcgg gtagtgtttg gaacatcctg actcgtttga acaccgacat
    15301 agtacatcat tttcccgtta tagggttcgt tgactgtaaa gaaataatga ttttcagcag
    15361 cttgtttcaa tgcatctatt gttccattac cgataacttt ttgctcaaat gcttgtttct
    15421 ctgcagccaa accgacaaaa tccgtattgc tactcttcaa ttccacacca atacccgtca
    15481 ccgtaaaatc tggtttttct tcgatcctat actttgccat ttctcccacc ctttctatta
    15541 tattaagttc atcttacata aaattaaagg gaaatgacac gattacgctc ttttttctag
    15601 ggatattttt attttttaac tatttttttg ctttcactgc aatccaaatc tcactgtaaa
    15661 cattactgaa atcctctccc cattttgtaa aagagatctc gggagcttcg actaactcat
    15721 aatctgaaga aggaagccat tctgcataga ttttccccca agtatcttgt aaagtttggg
    15781 gaaatggccc tttattagga aaaatggccc aagtgtgttc ttctatagca agcatttcta
    15841 aatcagcgta cggattttct tttgtcgtag caaagccaat catgtgcatc aaactgccct
    15901 tttcttccaa gcgggctcca tcaaatccat aagaaacgtt gattacttga tttggatata
    15961 aatctcctaa cttatgcatc tcttcccttt gagtctccgt gatcgtctgc gctaactttt
    16021 gtatttcttg attgactcct tcaaatttga caggaacttt tttcgccaca cctgccacat
    16081 aaaactgttc tttcttttcg atacgaaatt ccatcgattt tcctcctctg acatcaataa
    16141 aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct gctgatttct gaaggcagat
    16201 atcctgacca atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat ccatatctaa
    16261 acgccgtttc tgtcactctt gatccatcta ggagatcgcg attagccaaa gccaatctac
    16321 ggtttttgat atagtccatt agcgacatcc ctgcaataaa cgtaaagatg cgttttaatt
    16381 gatattcaga gattcctgta ttttcggcta ttattttata ggaaagtcct tctgtcaaat
    16441 gttcttcgat gtaatcgatc aattggttca gatctttaag catatttatc ttcctccttg
    16501 cttgcctttt tatcatatta tcaaagaaag tcaattactt gatgaatcag gaaccaattt
    16561 agtcagatgt agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc gtttcgtaac
    16621 ggataattgg tattatctgc ctatctatgt tttaccttta gaaaaataaa ttttggggaa
    16681 gacacattct aagtaaacaa agcggtaaaa atatctttta ttcccttagt taatccgctt
    16741 actacctact gtacaaagca aattgttgtt caaactgctg atcagaacgc tgcttggatt
    16801 tttctttgcg atacatcata cctcttttat caattggaac aattatttat ttctaacaaa
    16861 atcacttcat ttttgtcaga taaataaaaa acaaaaaaag ttccatcttt aaaacacgct
    16921 attcttttac tttttctcac attctctaag aaaagaaaag gagaatgaat ttttgacaat
    16981 tcgagcaaaa gtgattatca aatatgtaaa atcagctact tttttcttaa acgtttcttt
    17041 atactaaata taaataagtg tttcatgaaa ggagaactag atgaataatt ggcttgagta
    17101 ttttcctgaa aacgttttgg aacgcggtta cagctatcat ctgcacggtt ttgtccgtca
    17161 tttgaactat acaagcaagt acttgtccgc aacagtttct ggcacggaag attataaggt
    17221 agtgatcact tgggatgaaa agaccaacat gacttgtgat tgtctatatg ccatagaagg
    17281 aaaaaaatgc aaacacatgg ctgctgtctt attcgcttac gaagaacgtc caataaaaaa
    17341 atccaactac tcactttccg aactctcgtc ccttgtttcc tccgctagtt cctctcttgt
    17401 acgggagctt ttaacagaga ttcttataga acatccgcaa tttatcgaac gcttcaaagt
    17461 taagatgcca tttcatgcaa taaattattc agataaatta acaactatca tccataaata
    17521 cgatcatatt atcaagaaaa acaaaaacag gaaaacagcg aagttcatca tggagatgcg
    17581 aaagttcatc caagaagcag ttgaatcgct cattcagcaa aatgcctatc ttcccgcatt
    17641 cgaattgatc aatgaagtga ttgctactct cgaaacattc tattgggagc ctgaggatga
    17701 acgaaccctt cttctgatag aggactgtta ttatttatgg aaagaattat tagcagaagc
    17761 acctcatgca gaaaaaaggc aaatgttctc ttggttcgtc tgccaagtcg atcatactga
    17821 cgcctcttat tcaaaacgct actccataaa aatcctgaaa
//
LOCUS       DASJON010000042        17215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJON010000042 DASJON010000000
VERSION     DASJON010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.042"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..706
                     /locus_tag="U3989_001042"
     CDS             <1..706
                     /locus_tag="U3989_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1714504.1"
                     /translation="SVVDDILVVLVLSFSLSFLTGEASSNASLPLVLIEQLIYFVFIF
                     LLVKWIAPFLLSFAEKVYANSSIIIVSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQ
                     TKVKEEVYHNVEALGYAVFIPVFFVSVGLEVDFSDFSEQLLFISILTLVAILTKLLGG
                     YFGAQIAGFSQHSSLMVGAGMISRGEMALIILQIGQQSQLIEPQYYSPLVIVILLSTL
                     ISPLVLKYFTKKVYEV"
     gene            complement(815..2857)
                     /locus_tag="U3989_001043"
     CDS             complement(815..2857)
                     /locus_tag="U3989_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN1714505.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADDKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2915..3472)
                     /locus_tag="U3989_001044"
                     /pseudo
     CDS             complement(2915..3472)
                     /locus_tag="U3989_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(4011..4217)
                     /locus_tag="U3989_001045"
     CDS             complement(4011..4217)
                     /locus_tag="U3989_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714506.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(4233..4847)
                     /locus_tag="U3989_001046"
     CDS             complement(4233..4847)
                     /locus_tag="U3989_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN1714507.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(4994..5677)
                     /locus_tag="U3989_001047"
     CDS             complement(4994..5677)
                     /locus_tag="U3989_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1714508.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(5786..6244)
                     /locus_tag="U3989_001048"
     CDS             complement(5786..6244)
                     /locus_tag="U3989_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN1714509.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(6328..8091)
                     /locus_tag="U3989_001049"
     CDS             complement(6328..8091)
                     /locus_tag="U3989_001049"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN1714510.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(8312..9772)
                     /locus_tag="U3989_001050"
     CDS             complement(8312..9772)
                     /locus_tag="U3989_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714511.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(9980..10906)
                     /gene="glsA"
                     /locus_tag="U3989_001051"
     CDS             complement(9980..10906)
                     /gene="glsA"
                     /locus_tag="U3989_001051"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN1714512.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(10929..12356)
                     /locus_tag="U3989_001052"
     CDS             complement(10929..12356)
                     /locus_tag="U3989_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1714513.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(12387..13601)
                     /locus_tag="U3989_001053"
     CDS             complement(12387..13601)
                     /locus_tag="U3989_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN1714514.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            13951..15030
                     /locus_tag="U3989_001054"
     CDS             13951..15030
                     /locus_tag="U3989_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1714515.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(15331..15573)
                     /locus_tag="U3989_001055"
     CDS             complement(15331..15573)
                     /locus_tag="U3989_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1714516.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(15605..16162)
                     /locus_tag="U3989_001056"
     CDS             complement(15605..16162)
                     /locus_tag="U3989_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1714517.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(16175..16363)
                     /locus_tag="U3989_001057"
     CDS             complement(16175..16363)
                     /locus_tag="U3989_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1714518.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(16376..16942)
                     /gene="amaP"
                     /locus_tag="U3989_001058"
     CDS             complement(16376..16942)
                     /gene="amaP"
                     /locus_tag="U3989_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1714519.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4891 a         3610 c         3020 g         5694 t
ORIGIN      
        1 ttctgtggta gatgacattt tagtcgtttt agttctaagt ttcagcctat cttttctaac
       61 gggggaagct tctagcaatg cttctttacc gcttgttttg attgaacagc taatttattt
      121 tgtttttatt ttcttattag tcaaatggat tgcgcctttc ctactttcat ttgcagaaaa
      181 agtgtacgca aattcttcta ttatcatcgt atcgctagtt atttgtttag gaatgtctta
      241 cgtagcggat cttgttgggc ttagctctgt cattggtgct ttttttgctg ggatagctgt
      301 cagtcagaca aaggttaaag aagaagtata ccacaatgta gaggctttag gatatgcagt
      361 atttatacca gtattttttg tcagtgtagg attagaagtt gatttttctg actttagtga
      421 acaactactc tttatttcta tattgacgct tgtagcgatt ttgaccaaat tgcttggagg
      481 ttattttggt gctcagattg ccggtttttc tcaacatagt tcattgatgg tcggtgctgg
      541 tatgatttcg cgtggagaaa tggctttgat cattttacaa attggacagc aaagtcaatt
      601 gatcgaacca cagtactatt cacctttagt tatcgtgatt ttattaagta cacttatttc
      661 tccacttgtt ctcaaatatt ttacaaaaaa agtttatgaa gtgtgagtaa atcaaaaaga
      721 agttgtgaaa gagacatcgt gaaaaccgtt tatttgaaaa aaggactgtg acgataacgc
      781 cacagtcctt ttactgtact taaaaagcaa ttttttattg ataattttgg ttgaggtatt
      841 gcaatagttc cgatgctcgt ttggttgctg aatcatcatc ttctttattt tccaacatgc
      901 tgaccatcat ataaccttgg ttatcaagat tgaaagcaaa caagaaactg ttctcttgcc
      961 cctttacatc ctgtttttct ttgatttccg ctgtaccagt tttcgctgcc aacggaatcc
     1021 ctaaagcaga aagagaatgt gctgtaccat ttacatcttg cacaacttct ctcagatctg
     1081 gcacgatcgt ttgtactgct gtttcgctga tgacattctt cttatctttt gtctctttat
     1141 catctgcaat caatttagga tagacaagtg tgccattgtt ggtaaaaaca gaatacattg
     1201 ctgcttgctg gataggagta attagaagtt cgccctgtcc atatccagta tcagctagca
     1261 agatatctga gttaaagcta tcttcattag aaatttgtgc tggattcata ctaattggca
     1321 aatcaaggtc ttcaccaaaa atgaatttat ctaagcctgt acgaaatttt ttctcaccca
     1381 ttttcaacgt ttcttgtgcc gcatatatat tatcggaata gatcaaagca gtttttaagt
     1441 ctacttgtga tacatcacta ctgacacgcg ttgttttaaa agatccccaa gaactgtctt
     1501 tttgccattt aagcccgttg atcgtcaaca cttcatttgg atcgatagtg ccgttgtcga
     1561 gaccgattgc tgctgtaatc attttaaacg tagatccagg agcataacct gtcgcaaatc
     1621 ggctgatgaa tggatgttca ggattttctt cataagcttt gtaatcttct tgtgagatcc
     1681 cgtttgtcat tttgtttgga tcatagcttg gagagctagc aagcgcaaga agatcaccgg
     1741 tttttggcgt tgtcgcaaca gttgatgcag cttttcctcc tagactgtca aaagctattt
     1801 tttgtgcctt cgcatcgatt gtcaatttga tatctttgcc attttgaact tcatgttcga
     1861 tcagaacttt tttctctaca ccgtctgcat ctgtaatgct taatttccca cctgttgtcc
     1921 cacgaagatc cttatcaaaa gccatctcca aacctgagcg accgattttt ccattactgc
     1981 ttaattctgg attcttatca atatcttctg ctgtgatatc tcccacgtaa ccaatcagct
     2041 gagcagctgc ttcacccaaa ggataatatc tgccgtctac ttcttgaatg gtagctccag
     2101 ctggaagttc tggcgttgct ccatcaatga ttttcaatgg gacaaagtaa tcgggttgta
     2161 cccagctttg tgaaatcgcc tgattgatag catcttctgt taagtcgaag gaggaagcaa
     2221 tcgctttgat attggctgtt ttttcgcctc catctccaag tttacttgga acgactccta
     2281 attgttttag tttgcctgtt gttgctaatg gttcaccatt tcgatctata atattccctc
     2341 ttgctgcttc ttgtgtggtc aggcttactt tgtcatttcc ttccatttct ggaaaaacca
     2401 agttgggttg ccagttgatc gtggtttgac catcatttct gtctaatgtt cctttataag
     2461 aaagatcttt caattcacct aatgaggtat tcatctttgc tttatatgaa aaactgtaag
     2521 tagaatcgtc ttttttatct acttttagat tcgatatctg aagtcctttg acatcagcag
     2581 caccgtatat attctggtat ttatctaaga tctctttttc agataatgca cttttatttg
     2641 ccgccttttt tgatgtcatt tctgcagctt tgttataatc tcccttgttc aaagcttgga
     2701 caaattgctc aactgtcttt tctccagctt ctactgcttg ggtttcttga tagtgctggt
     2761 agtaaaaata accgccaccc gcagctgcta ttaagatcac tgcgccggca atataagcgc
     2821 ctgttcgatt tttgccgtgc ttgtcacttc ttttcatttt ttcctccttc tcttgcttgt
     2881 cattccttta tttcactatt tatacctgtt tattctaaca tatgtctttg aggaaatcca
     2941 taccacaatc cagaagcaca ctttctggaa ggttcgtttg gatactacca agtaatttcc
     3001 caaccgcttt gggtagcttg aatattgaag ttacgtcttt cagctgctga gagatagctt
     3061 tgatcacttg ctgctgcctt ctgatcctcc cgaagtctcc ttctttgtct tcccgaaatc
     3121 gagcatactg gagtaaagta ttaccatcca tcacttgctc tcctttgttt atatatacgc
     3181 catctaaatc tagagatttt tctgcatcga tcttgactcc accaggaaac agttcattaa
     3241 cgcaatcgat aaaatcttga aatgtgatac ttgcataata gggagcttca aatttgaaat
     3301 tttcctttaa tgtttgattt aataaatcaa ctcccccata agagtaagct gcatttatct
     3361 tattcattcc gtaacctgga atatcgacat atgaatctct catgatagag atcagttttg
     3421 cttgttgtgt tttctggttg tattgtgcaa ccagaagtga atccgagcga cctccaccaa
     3481 tcgatctttt ttcaccgcat ctcctcctca cacagcaatc cacttgtcag gtgaggagcg
     3541 agtttgtctc atcaaataag tcagtcacaa aaatataaag aaaaggtcag aaaccttttc
     3601 gaacaagaat tctgacctag catcatctca tcgtccgagt tttagcacta tacaacgtaa
     3661 agaaaacatt cttagctatt ggaaaaagcc ttaatccaac agtttctgtt ttacccattg
     3721 gcgactttcg acgtttctgg gcaacagcct atgtttgtat aggagcctca cctaacaagg
     3781 ttattactat attctattta cgagttcatg atagactttt cctaatgatt aggcaatgga
     3841 atttgaaatg aaatagtgaa atttaacaat ctttaatatt ttttagaaaa aaataacaaa
     3901 taagttgaaa gaatcccgtt gcaaattctt tcagttgcac caggattctt tgataatttt
     3961 ttagttttat gtgttttttt atatgttttt tcacttttcg gaataaatat ttatttgtta
     4021 aagtaattaa acaatccgct aatcgtttga tctggcattt ctatttcctt accatctaaa
     4081 gtaacagtag taacgacaaa atggtcttgc aatggcggaa cttctaagta ctgcccttgc
     4141 agaaaatcat tcactgtttc atattctttt ttcatttctt ttgaatctgt cgatggttta
     4201 tagcttattt ttaacataac tagtgctcct ttttattcgg gacgtgcaat cgtgaaaatt
     4261 tctcctattg ctgcataatc atttcctgcc aaacggctta cagcattcaa ttttcttgga
     4321 tcgatccttc ctgtcttatc atcatagact gtttcattta aatgatattt ctgtacttct
     4381 agcaaaaata aatcagctgt tactttattt ttattccgta tctcgacatg ttcatataag
     4441 gatgtttcaa aacgaacttt tgcttctgtc aatgctggta ctgaaatgat tactgaatca
     4501 gttaaagtca agtcagtcag gtacagttcg ctttcctcat taggcagatt ggctgctgtt
     4561 ttatttactt cttctacgtt gtcctcatca aggatatgga taacagcttc tcttgtctca
     4621 agaatattct ttgctgtgtc ttttactgca tcttgttttc tctggatcgc caaggataag
     4681 atgggtcgat tcgaactgac gatattgaag taactgaagg gtgcgatatt cactatatct
     4741 tttgctgatt tagtcgttac tactgctact ggtctaggga tgactgaacc aatcagcaac
     4801 ttgtaatttt cacgttctgt caattctgaa ggactccgtt caaacatggt ttcgccttct
     4861 ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa tgcttttttc
     4921 atcttcttag aatctgcttt aaactatcta tcaatttttc ttttttgtca agaatctgtg
     4981 ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc gacatatctt ttagtgatca
     5041 attgcggacg ggtgataata gatccaacta cgactgaaaa agcacctaat tcatatgctt
     5101 tctctacttg ttcaggagag tcagtatgtc cttccataat tactgggata tcgacttgtt
     5161 ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa tattctgtat
     5221 agcctgctaa agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga agcgcttcct
     5281 caaaagtcga aatatctgcc atagctaacc gaccgtgttc gtgaatcatt tggacaagtt
     5341 cagcagttgt tttttctgtc ggacgtttac gagaagttgc atccagtgca atgatttctg
     5401 cttgcgtctc caaaagttct tccacttctt tttctgtcgc agtaataaac acttctgacc
     5461 catcgtaatc tcttttgata atccctatta ccggtaaatc aacgattcgc ttgatttcat
     5521 tgatatcttc tgtcgtattt gctcgaatac caactgctcc gccttctttt gcagccaaag
     5581 ccattctccc cataataaaa gaactgtgta gcggctcgtt ttccaaagct tgacaagaaa
     5641 cgacgactcc gcctttgatt tgttccagtg tatccacaat atctcctcca gtaatgttat
     5701 ataaaaatac agcttcatat aaaaatgtaa cccttttcta ttcaggagac aataatgagc
     5761 tatttctttc agcagacctt tcttactaaa agaaaattac ttcaaattct tcgtatacga
     5821 ccagcttctc ttctgtaaat tctatgttca acgcttcttt ataagcatgt ttaaaaaact
     5881 cagcatggac tttccgccaa taagctaatg tccgatcccc ttctccttct ttccaagcat
     5941 gctctttcgt gacttcacta aaaggcaaga tggtcacatt tatatttttg atgatacaaa
     6001 ctggttcatt ttttccattc aggaccacgt ctaattgacc aacttttggt aaaggctcat
     6061 tctcttgttt atacagatcg taaccacttg atgtaccagt ctttatcttt tttacaacta
     6121 gttcgctcaa ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa cctaattttt
     6181 ctgattgata actaggatat ttttcttgga actttttgac atattcttct acttgctgat
     6241 acataacatc cacccctttg ctttttataa tgaaaaaacc atccagatac tagtataaac
     6301 aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca gactatcttc
     6361 aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag gaacgctatc
     6421 cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac cataataacg
     6481 actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga tgactaattc
     6541 gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg ttcctggatt
     6601 gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt ttcctaattc
     6661 aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg tttgataggc
     6721 tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg taggaattcg
     6781 gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga tcatatcctc
     6841 ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag gaatgtcaaa
     6901 tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta aaataatcac
     6961 tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt gcaagaaagt
     7021 acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag cgaaagtgat
     7081 agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt cttggaatgt
     7141 tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag ctgtacttgt
     7201 ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc cagaagttac
     7261 ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt tcattttatt
     7321 atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta caatcggatg
     7381 atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt ttccattatc
     7441 cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt taccgttggt
     7501 ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa tagacatctc
     7561 attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt tcaatccttc
     7621 aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat ttcctgattc
     7681 tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg tagatgtgtg
     7741 ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc tcacttcacg
     7801 gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac cataagactg
     7861 atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat gaaacgcaat
     7921 cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc tgtccttttg
     7981 caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag ataatttttt
     8041 gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca taatacattc
     8101 tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat ctattctact
     8161 acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa gcttgttata
     8221 taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac aattaaaaag
     8281 ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt aataggtgaa
     8341 gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt ggatagatct
     8401 ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa accactcttt
     8461 ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa ataggattta
     8521 tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa aattgtttga
     8581 ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt tcactgttga
     8641 tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt agttggattt
     8701 gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga agttgttctt
     8761 tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc cacaacgtac
     8821 ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg atttcagcga
     8881 tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca aaatttttat
     8941 aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa tttctactgt
     9001 tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt cctttttgct
     9061 cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt ttcttatctt
     9121 cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt cctcgtatga
     9181 taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct acataatcaa
     9241 tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct tcccaattga
     9301 cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata atgatttctt
     9361 tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg attgtatata
     9421 ctttgctcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat ataccattaa
     9481 aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag
     9541 aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt
     9601 ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag
     9661 tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt
     9721 cacacagata cacttttcta aatatatttc gctcgatgta attttccagc attcctgata
     9781 cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt
     9841 cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc
     9901 aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc
     9961 cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt
    10021 tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac
    10081 ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag
    10141 gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa
    10201 ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa
    10261 ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg
    10321 agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac
    10381 ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga
    10441 tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc
    10501 gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg
    10561 cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat
    10621 tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt
    10681 ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat
    10741 cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt
    10801 gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat
    10861 agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct
    10921 ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga
    10981 ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca
    11041 gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga
    11101 cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca
    11161 agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc
    11221 aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg
    11281 cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaatga
    11341 gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct
    11401 gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct
    11461 aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt
    11521 aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact
    11581 gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca
    11641 aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg actggcagat
    11701 gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc
    11761 gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg
    11821 acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa
    11881 ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc
    11941 cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata
    12001 cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt
    12061 atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg
    12121 acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc tgctgcgcac
    12181 aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag
    12241 cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc
    12301 aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat
    12361 cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc
    12421 aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt
    12481 aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga
    12541 atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc
    12601 cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca
    12661 tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg
    12721 atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc
    12781 aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt
    12841 gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca
    12901 ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac
    12961 caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct
    13021 aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc
    13081 gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca
    13141 ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata
    13201 atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc
    13261 acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac
    13321 atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca
    13381 ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt
    13441 atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat
    13501 ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc
    13561 atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta
    13621 cgattacgag tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac
    13681 ttgcatttat aaaagacaga caattaataa aagcttgatg tgttgtgctc tttttcgttt
    13741 ttcacacaaa taaagacaaa aaataaacgg tcttcttttt atagaaaacc gtttattttc
    13801 gtcttttttt atttttaaag atattttttc ataaggaata aagaggcgga ttttataatt
    13861 aacttagcca tttggaaata aaaaaacgcc acgtgaattc catgttatat tgaagttacc
    13921 acaactctca atagacaagg atgaattcac atgacgcaaa accatgataa cacagtttca
    13981 cgcaagggaa aacacctaac ctactcagaa cgttgccaaa tcgccatcct gaaaaaggaa
    14041 aactattcca accgtcaggt tgcggatgtc ctgggctgcg tgccgcagac cataaacaac
    14101 gaaatcaaac gcgggaccgt tactcagctt aaccgccaga atcagaacgg gaaggtctac
    14161 gactattatc atgacgtcta cgacgccgat gccgggcagg cggcctacga gaagaaccgc
    14221 ttgaactgcg gcagacggcc cttatgggcc gacacggacg ccttcattga atgggcggac
    14281 gacatgatgc tgttggaaaa atggtccccg gacgtggtgg tgggctttgc cagaaaacac
    14341 gaattgttcg acccggccat tatccctggg accaccaccc tctatcagtg gatcgaccgt
    14401 ggcatcatga aaacgaagaa tatggacctg ctagaaaaac tgtcccgcaa accgaaatcg
    14461 tcccgtcatg gcaaccgccc gaataaacgg gtgctaggga aatccattga agagcgcccg
    14521 gaggcggttg gtacccggaa aaccttcggc cattgggaag tcgacacggt cgtaggaaac
    14581 aagaccaagt cggatgccgt gctgctgacg ttggtggaac ggcagacacg cttcgaggtc
    14641 attctgaagc tggccgggaa ggatgccgaa tcggtcaacc aagcggtccg acagctgaga
    14701 gaacgggctg gtgcgtactt cggccagctg ttcaagacgg ttacctcgga caacggctcg
    14761 gagttcgcgg gactgcacga tgccttgcag gatgtggcaa acatctactt cagccacccc
    14821 tatgcttcct gggagcgcgg gacgagcgaa aaccaacaca agctgattcg ccgtttcatc
    14881 cccaagggca gcccaatcgg caacgtgagc gaaaaccaaa tcttgcggat tcagcgttgg
    14941 atgaacgatt acccgcgaaa gattttgggc tatgcaacgc cgcatgacac gttcgtccag
    15001 gcatttaggc aggaacggtt gacggcttga cacgtgggcc cttgacaacg agcctccctc
    15061 ggcatggctt gtgtctggaa agctggccgg ggccagccag tcgggagact accatgcctc
    15121 agctcattgt caagggccca ctcgattaga aaaattagac ggttggtggt aacgagcaac
    15181 cgggtcacca tgggtggcta acttaaactt gaaatttacg taaggaataa agaatacttc
    15241 atataaaata tataaaaaag aaccgcagcg agtgtcgcta cggttctcaa atttgttctt
    15301 catttcaaca taagtttttc ttcaatttgc ttataattta cgtgcaaaga atgaaacgac
    15361 taatactaaa atgattgcac cgatcaatga tggaatgata gccattcctg caattgttgg
    15421 tccccaagtt cctagtaaag cttgtccaat tgctgaacct actaaaccag caatgatgtt
    15481 tgcgatgatc cccattgatg atcctttatt cgtgattgct cctgcaattg ctccaatgat
    15541 accgccgacg attaatgccc aaataaatcc cattttaaat tcctcctaac tgacttctta
    15601 cttcttattg tacacgtggt tcgttatttt ctttgacttt ttgtgcacct ttgcctacag
    15661 cagattttgc tttatctgtt tggcgtgaag caaattgtcc tgtggatttt gctgcttctg
    15721 ttactttgtc ttgtactgtt tcttgatctt tttggtattc ttcttgtgtt ttgatgtctg
    15781 caacattcac attcacttcg ataacatcta aatgtgtcat attgtttact tcagtactga
    15841 tcaattcttt gatttggttg taaatatctt cgatgtcttt accgtattct acgatcacat
    15901 ctagatccac agctacttgt ttcttaccta cttctgtatt gatacctgct gttacgttat
    15961 ctgtgttgac taatttttca gccatatttg agaaaaatcc gccatctaat gtcaatagac
    16021 catctacatc tttcaatgca taaccgatga ttttttggat gactttgtct tcataagtca
    16081 attcaccatt catcatgcgg ttttctcctt tttcatttgc caaaactgat ccatttgttg
    16141 tattcatgtt tttgttttcc atctttgttt cctcctatga tttaaaaaat tgatcaacta
    16201 ttcctgactg tttcaaataa aaaccgattc ctgcgccaat tgctgtcatg attatgatca
    16261 acaatgtctt gaaaaatcca atcgttaacc ataaaacagc tagcagtagt ccaagacccc
    16321 caaagataat agggagcttg tatgctgaaa atagttcttt cattatttgg ccctcctatt
    16381 ccactcgaga gtgcttgtag tctcttgtat cttcttgttc ttcataaccg ctgtatgtga
    16441 ctgccacttt gaccgtttct tttgttccga gtactttagt tacttcttct tcgatatctt
    16501 gcatcaatac acctgttttc cctactaatg aagaagttcg tttcaagtct ccgttgacat
    16561 ggatatggat tcgatttttc gttgaacgta tccgtacttt aggtgaatga atgaactctt
    16621 cttcgtgtaa atgggaacgt accaaccctt cgatcgcttt tttgtcgatt gttagtttgc
    16681 catcttcccg tttcataacg aaagttcctc ttgactttgg atagaaaaga acgactagca
    16741 agatgataat agcaagaacg gcaaatacta cagctgccca gaaaatatat tgttgcatgt
    16801 agtaattcgt taaagtaaag aagcgaaatc gttccaattg ttcaggcata atatacggcg
    16861 cattgatcaa cgcaatcgct agaaatactg aaagtagcag taaactcatg ataacaccga
    16921 ttatttttaa ccccttattc attttccacc tcctataaac ttatttttac cctgtaaata
    16981 actctaccga caatcacagt atagatagcg aacagttagc atagcatcag taaataaagc
    17041 gctaccaaac attgctggat cataacggcg ttttattgcc agtctgatac tgaaataaca
    17101 attgcttact tattctctat ttacttttga ttgctggcta tacttaaagg atattattca
    17161 agattaaaaa ttgcaaagat tatgctcagt tattattaca agattctatt tattt
//
LOCUS       DASJON010000043        17068 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJON010000043 DASJON010000000
VERSION     DASJON010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.043"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1216
                     /locus_tag="U3989_001059"
     CDS             <1..1216
                     /locus_tag="U3989_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1714520.1"
                     /translation="SSTKKEPIIPEKKITEKAKEPTDDKQQLVTQAKNLAQSYDYDQA
                     IALLEKDSKNGSTETQQLLQTLKKEKEQLIDWSDPTQISHVFFHSLIVDPQKAFHSQQ
                     AQGYKDYMVTVEEFNRSIEQLYQNDFVLVNLNDLIQKDEQGNLSFNGLALPEGKKPLV
                     LSQDDVSYYEYMDNSGFPSRLVVSNNNEIKNVYKEKDKEEIGDFDMVPLIDAFVKKHP
                     DFSYRGAKGTLALTGYNGVLGYRTSKSQYGDNEKTKKEIKEAKKVAEQLKKDGWSFAS
                     HTWGHLNMTQASLEDVKKDNELWQQEVAPILGKTNILIYPFGADISDWQPYSAENQKF
                     AYLKSQGFDIFCNVDASTPAWGQLGTDFYRNARINIDGIRFQSDLDGKNPILDPFINV
                     HDVYDKEARESA"
     gene            complement(1285..1884)
                     /locus_tag="U3989_001060"
     CDS             complement(1285..1884)
                     /locus_tag="U3989_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1714521.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            2138..3019
                     /locus_tag="U3989_001061"
     CDS             2138..3019
                     /locus_tag="U3989_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714522.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            3032..4642
                     /locus_tag="U3989_001062"
     CDS             3032..4642
                     /locus_tag="U3989_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN1714523.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(4719..5378)
                     /locus_tag="U3989_001063"
     CDS             complement(4719..5378)
                     /locus_tag="U3989_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1714524.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(5661..6326)
                     /locus_tag="U3989_001064"
     CDS             complement(5661..6326)
                     /locus_tag="U3989_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN1714525.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(6473..7078)
                     /gene="dhaL"
                     /locus_tag="U3989_001065"
     CDS             complement(6473..7078)
                     /gene="dhaL"
                     /locus_tag="U3989_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN1714526.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(7106..8116)
                     /gene="dhaK"
                     /locus_tag="U3989_001066"
     CDS             complement(7106..8116)
                     /gene="dhaK"
                     /locus_tag="U3989_001066"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN1714527.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(8091..8537)
                     /gene="dhaM"
                     /locus_tag="U3989_001067"
     CDS             complement(8091..8537)
                     /gene="dhaM"
                     /locus_tag="U3989_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN1714528.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8640..8960
                     /locus_tag="U3989_001068"
     CDS             8640..8960
                     /locus_tag="U3989_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN1714529.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(9072..9941)
                     /locus_tag="U3989_001069"
     CDS             complement(9072..9941)
                     /locus_tag="U3989_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1714530.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(10037..11341)
                     /locus_tag="U3989_001070"
     CDS             complement(10037..11341)
                     /locus_tag="U3989_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1714531.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(11338..11847)
                     /locus_tag="U3989_001071"
     CDS             complement(11338..11847)
                     /locus_tag="U3989_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714532.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(12022..14157)
                     /locus_tag="U3989_001072"
     CDS             complement(12022..14157)
                     /locus_tag="U3989_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1714533.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(14240..15895)
                     /locus_tag="U3989_001073"
     CDS             complement(14240..15895)
                     /locus_tag="U3989_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1714534.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(15897..16211)
                     /locus_tag="U3989_001074"
     CDS             complement(15897..16211)
                     /locus_tag="U3989_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1714535.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            16381..16641
                     /locus_tag="U3989_001075"
     CDS             16381..16641
                     /locus_tag="U3989_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN1714536.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            16655..16969
                     /locus_tag="U3989_001076"
     CDS             16655..16969
                     /locus_tag="U3989_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN1714537.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         4788 a         3504 c         3087 g         5689 t
ORIGIN      
        1 atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga
       61 accgacagat gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga
      121 ttatgaccaa gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca
      181 acagcttctt caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac
      241 tcagatctct catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc
      301 tcaacaggca caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat
      361 cgaacaactt tatcaaaatg attttgtttt agttaatttg aatgatttga tccaaaaaga
      421 cgaacaagga aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt
      481 tctttcacaa gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg
      541 attggtcgta tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga
      601 aatcggtgac tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt
      661 ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta
      721 tcgaacatca aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc
      781 taaaaaagtg gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg
      841 tcacttgaat atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca
      901 gcaagaagtt gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga
      961 catcagcgac tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca
     1021 aggtttcgat attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac
     1081 ggatttctat cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga
     1141 cggaaaaaat cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc
     1201 acgcgaatct gcttgatttt acgtcattta tgaaccaaaa aagccgaact gacattttat
     1261 cgtcagtttc ggctttttta tgctttattt cttcttcggt gcgattccct tgatcaggac
     1321 ttctgtccat tctgctgtat atggaagcag ttttgcgtat atctccggct tagaaattcc
     1381 ttctaccatt gcttgaaaat agaggatcaa gaactcatcc gaatatttca aatccacttt
     1441 tccttctttc cgccctcgat ggaaaagatc aagcatgata ctgaaacttt cttgcgtata
     1501 cgcatccatc gcgtcagaaa attctttttt ttcttttttt gtatagtatg tcatcaaatc
     1561 gatataaaat tgtttgttca ctcgattgag ataacggatt ttgttttcac tcatggcaat
     1621 cagtgtttct tcaaatggaa gattttccgc aatcaacgct ttcgcttctt gccctaaatc
     1681 attcataaat gcaaaaaata cttcataaat cagcccttcc ttgctgccaa aatatttaaa
     1741 aatcgtcgct ttacttacct gagcgttctc agctacctta tccattgtca ctccagcggt
     1801 tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa atctctttct ttttctcttg
     1861 tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt aattttaaac ttttataata
     1921 tatatagttc aaatttattc tgaggtttat tataactatc tattgagaaa gtatcaagct
     1981 agcaattcct tgtttatgaa ttcattgaca gaaatttata aaaagaatat tatgtactaa
     2041 aataataaaa aaagttttat aaaaaggtat aaaacctttt tattaaacta tttaatcttg
     2101 tttagtatat atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac
     2161 acatttgaaa aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca
     2221 ttcaggtgaa gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg
     2281 gacactttta gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt
     2341 atgggaagat agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt
     2401 gtggggaaat ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg
     2461 gagtgctcaa aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc
     2521 aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga
     2581 ttcggatctc tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat
     2641 tttccaagaa gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca
     2701 tattctcagt gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact
     2761 cgatgaagaa ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga
     2821 aacagaagca gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa
     2881 aggtcacctc gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc
     2941 gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat
     3001 gagacattat gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac
     3061 ccttttttct caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt
     3121 cactgctttt tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt
     3181 agctggatta tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc
     3241 agcttcgaat gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt
     3301 ttgcagtttg tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa
     3361 agaagaactt ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc
     3421 tttagctgtc atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg
     3481 tacgatgatt tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct
     3541 atctgtaggt agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat
     3601 gccaagttca gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct
     3661 acgagcaata acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc
     3721 ttatttgacc tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt
     3781 cagtctaatc ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg
     3841 ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg
     3901 tttattattc tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat
     3961 gggggcggca tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt
     4021 aatgaaacag atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat
     4081 catggtagtc ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt
     4141 tacagaagaa acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca
     4201 actttattgg accaatatgt tactagctat tctatttgct atattggcaa tcctgattag
     4261 ttctggtgca ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct
     4321 attgatgttt cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat
     4381 tagtggcttt ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc
     4441 ttactccttt gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa
     4501 aacagctgtc cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt
     4561 ccttacgtta tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg
     4621 aagagactta ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc
     4681 tgggtcaaaa atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct
     4741 ttatcaatga tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat
     4801 ttaaattttc tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt
     4861 ttcattggca ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca
     4921 gcgactcgtt cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt
     4981 aatttctcaa cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt
     5041 tccgagaaat ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat
     5101 aaagactcat ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt
     5161 actcgtaaaa gctgttcctt tccgctagca gataattgat acactttcat actgccggag
     5221 gccacgatca ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca
     5281 tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac
     5341 aaaggaacaa atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag
     5401 ctatttctaa taaagaaaca taacttccct tattccaact gataattttc ttttatcata
     5461 gcataaacgt caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc
     5521 tttattttcc ttcttttgag accttaagta attgattcct atcaattttt taaatattta
     5581 ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg
     5641 cattgcttat ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt
     5701 gctagttctt gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag
     5761 aagaatagtt tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt
     5821 ttctttgtat cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt
     5881 tgcggccata tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca
     5941 agcaactggt tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac
     6001 atcaaataga tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct
     6061 tgacgagatt cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc
     6121 cagctttcga ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg
     6181 agctgaatct ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc
     6241 ttatctgttg gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac
     6301 tgataatctg attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta
     6361 cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat
     6421 aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt
     6481 gattgatcac atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga
     6541 tatgtccgat tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt
     6601 tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct
     6661 ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc
     6721 tttgaatacc cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag
     6781 aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag
     6841 aagccccacc gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg
     6901 tttcaggatg atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat
     6961 ttccatggtc tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat
     7021 tgattgcctc tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat
     7081 tatcaccctt ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag
     7141 aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta
     7201 gtgaggtcat aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca
     7261 gagctaaaac atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt
     7321 tgactagtac acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa
     7381 gttcctttgc cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt
     7441 ggataccaac accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt
     7501 ctggtacagt tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt
     7561 ctcccagttc tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt
     7621 tgtgtacaag gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa
     7681 cggcgatatc atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt
     7741 cctttgccat atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc
     7801 cttttccact atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa
     7861 acacatcacc aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg
     7921 gttcatgtcc gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg
     7981 ctacaacacg ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt
     8041 ccagcatttc ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat
     8101 cctttctttc cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg
     8161 gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt
     8221 tgctgctgct cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg
     8281 atatccgcaa aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt
     8341 ttcatcgggt ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct
     8401 tcggattttt gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga
     8461 gaaataagca aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg
     8521 attggtttcg ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa
     8581 aatatgtgta gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa
     8641 tgattacaac aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg
     8701 tttttggtga ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa
     8761 atatctttgg cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag
     8821 cacttgccga aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa
     8881 aaatggacta tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa
     8941 cagctgtctc cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac
     9001 atgtagtggc actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca
     9061 tcctctcttt tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc
     9121 gcccctgcta aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga
     9181 cttgttgatt tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc
     9241 actgattttt ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag
     9301 tcagcttttt ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg
     9361 attgcttgac tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt
     9421 tgagcaacct gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga
     9481 tctgtttgga cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca
     9541 cggtcatttc ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct
     9601 tctttcaatt tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc
     9661 aagtcttgca aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct
     9721 gaagatgaaa tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc
     9781 gaggcaccgt tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt
     9841 tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc
     9901 cagattaacg gcttcattct ttttttcatc acttttccca tccttactac tttttaacta
     9961 atttcttatt ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta
    10021 aaaaacggtg catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat
    10081 aacctaaaat cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta
    10141 tccagaacaa aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat
    10201 catctacaat cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga
    10261 acacaaaact gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat
    10321 gcccgattaa tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac
    10381 tcaatgtttg atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca
    10441 cttttttgaa attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc
    10501 gatatagcat cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa
    10561 cagataacca gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat
    10621 cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac
    10681 tgtacgcata aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac
    10741 ttgagaattc aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat
    10801 ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa
    10861 tcagtgcatc tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg
    10921 caaaggcaag tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca
    10981 cacgaagatt agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg
    11041 aaccatcgtc tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact
    11101 cgattgtata ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg
    11161 gttcgacact caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat
    11221 aacaccagac accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa
    11281 aatacataat agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca
    11341 tttcagttcc accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt
    11401 ctcttcggag acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc
    11461 cgtatacact tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa
    11521 agatttattt acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt
    11581 aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt
    11641 ttctgataaa aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa
    11701 aaagacccat ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg
    11761 ccaaatcttt aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc
    11821 aacttcttct cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag
    11881 gagtttacca tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa
    11941 aagaacaaaa catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc
    12001 tttttatttt ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc
    12061 tctagatatc gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa
    12121 tggatataag attacatgat caaattccag tccttttgct agactcaaag gaatgatttg
    12181 gcagttttct aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc
    12241 ttctgtttgg gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag
    12301 tattttcaac aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg
    12361 tcggatcgat acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt
    12421 gattgctccg ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta
    12481 gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt
    12541 ctcgtcacct actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg
    12601 tgccagggta taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac
    12661 cttttcaaca agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt
    12721 gggttgccaa gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg
    12781 ctcatccacc catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa
    12841 aagtttcctt gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa
    12901 taattccaac tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt
    12961 tgcttgtttc aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag
    13021 cctatcgatc aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt
    13081 agaaaggact gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa
    13141 caaaggatct gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg
    13201 ctccgataca ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc
    13261 tgctgaaagc tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa
    13321 tgatggtagg acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa
    13381 gtcatcggat gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat
    13441 agcagaggtt ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc
    13501 ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt
    13561 ctcatcttgg cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa
    13621 aaacttgacc aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc
    13681 gattttattt tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc
    13741 ggcaattggt gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc
    13801 tgtaccgata taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc
    13861 aaagtaggga cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg
    13921 tttgatattc atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag
    13981 gttatctaag taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg
    14041 gtttccttct atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag
    14101 cttttgatac acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca
    14161 cattctcctc tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat
    14221 gcaagctttt taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt
    14281 gatataaaga aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc
    14341 tttccgctag atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt
    14401 ctaattcatt taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt
    14461 tgcccttttc caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt
    14521 ctgtcggcaa ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg
    14581 catatcctag catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt
    14641 ctaattccgg taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga
    14701 tccgtttatc cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg
    14761 gatggaagat caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt
    14821 ttcctgatac atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta
    14881 aaccggtgga tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt
    14941 gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg
    15001 gtgaagcaag agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat
    15061 cccccaccgc ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc
    15121 cgcgtatgcc taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa
    15181 tggataaaat aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc
    15241 cgtggtcttc aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca
    15301 attctttttc tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt
    15361 gttcgatcac catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga
    15421 tgaatcctgc accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt
    15481 tatcgatatc tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa
    15541 tcgatggaac aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt
    15601 gtttttgacc ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt
    15661 catgatttgg acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa
    15721 gaagattttc ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa
    15781 aagaaacata cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa
    15841 gccgtgtagc agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat
    15901 ttccctccta ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca
    15961 taaccagatt tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc
    16021 acatagacgg tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt
    16081 ttagctcctt ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct
    16141 ggattttcca aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat
    16201 agcaaattca ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa
    16261 acaggagctt tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat
    16321 atttgcataa aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt
    16381 atggagattg ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt
    16441 ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc
    16501 gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat
    16561 tgctttgaga aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc
    16621 aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga
    16681 attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga
    16741 aacgacgatt gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa
    16801 tgcagaattc ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc
    16861 taatcatatt tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct
    16921 tgatttagtt gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt
    16981 tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg
    17041 gaaaaccgtg aatcaaaggt tgtgaaaa
//
LOCUS       DASJON010000044        16821 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJON010000044 DASJON010000000
VERSION     DASJON010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.044"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(169..1455)
                     /locus_tag="U3989_001077"
     CDS             complement(169..1455)
                     /locus_tag="U3989_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1714538.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1448..2716)
                     /locus_tag="U3989_001078"
     CDS             complement(1448..2716)
                     /locus_tag="U3989_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1714539.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2968..3429
                     /gene="mscL"
                     /locus_tag="U3989_001079"
     CDS             2968..3429
                     /gene="mscL"
                     /locus_tag="U3989_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN1714540.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3488..3934)
                     /locus_tag="U3989_001080"
     CDS             complement(3488..3934)
                     /locus_tag="U3989_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1714541.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4027..4272)
                     /locus_tag="U3989_001081"
     CDS             complement(4027..4272)
                     /locus_tag="U3989_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN1714542.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4387..4965)
                     /locus_tag="U3989_001082"
     CDS             complement(4387..4965)
                     /locus_tag="U3989_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1714543.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5042..5572)
                     /locus_tag="U3989_001083"
     CDS             complement(5042..5572)
                     /locus_tag="U3989_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN1714544.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5576..6436)
                     /gene="rlmB"
                     /locus_tag="U3989_001084"
     CDS             complement(5576..6436)
                     /gene="rlmB"
                     /locus_tag="U3989_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN1714545.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6429..6824)
                     /locus_tag="U3989_001085"
     CDS             complement(6429..6824)
                     /locus_tag="U3989_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN1714546.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6821..8233)
                     /locus_tag="U3989_001086"
     CDS             complement(6821..8233)
                     /locus_tag="U3989_001086"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN1714547.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8230..8766)
                     /gene="cysE"
                     /locus_tag="U3989_001087"
     CDS             complement(8230..8766)
                     /gene="cysE"
                     /locus_tag="U3989_001087"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN1714548.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8852..9091)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9088..10548)
                     /locus_tag="U3989_001088"
     CDS             complement(9088..10548)
                     /locus_tag="U3989_001088"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN1714549.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10718..11875)
                     /locus_tag="U3989_001089"
     CDS             complement(10718..11875)
                     /locus_tag="U3989_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN1714550.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11909..13279)
                     /gene="radA"
                     /locus_tag="U3989_001090"
     CDS             complement(11909..13279)
                     /gene="radA"
                     /locus_tag="U3989_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN1714551.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13342..13869)
                     /locus_tag="U3989_001091"
     CDS             complement(13342..13869)
                     /locus_tag="U3989_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN1714552.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14099..14905
                     /gene="proB"
                     /locus_tag="U3989_001092"
     CDS             14099..14905
                     /gene="proB"
                     /locus_tag="U3989_001092"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN1714553.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14902..16149
                     /locus_tag="U3989_001093"
     CDS             14902..16149
                     /locus_tag="U3989_001093"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN1714554.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16225..16569
                     /locus_tag="U3989_001094"
     CDS             16225..16569
                     /locus_tag="U3989_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714555.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16686..>16821)
                     /locus_tag="U3989_001095"
     CDS             complement(16686..>16821)
                     /locus_tag="U3989_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714556.1"
                     /translation="ESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEETDLPGSW
                     "
BASE COUNT         4568 a         3601 c         3093 g         5559 t
ORIGIN      
        1 tcgtttgatt ttattttaat ataagttgat taaaagagag catgatttgt tttattttct
       61 cactaatttt tccaacacaa ctaagaatac cataaaagct gattttggag gaggttccta
      121 ctacaagtct aaaacgaaat ttaagacttt tcctcataaa atactggttt attttggtct
      181 catgtaaaac tcgctgaagg cctcttcact tataaatgct tccccagctg ccagtacgtc
      241 tttcatttgg atcgaatcga tgatttcagg aagatcaaat aaagttcgat cgccaaataa
      301 actttgcgta aattgattgg caatatattc gatcgaattt aatgattgga aatattgtcc
      361 aagcattttc ttcttcagca aatcgagatt tgtttctgac acttccggat catcagcgaa
      421 tccaagaatg atttctttga ctttttctgc tgctttctct ggttcgtccg tatctccgct
      481 gaagtccgca aagtgaaatt cacgatccaa gctgaattca aaaccaaagc tgtcatcgat
      541 aattccttga ttgtacatcg ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa
      601 caacagattg attgctgttt tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc
      661 taatccttta ataccaagta caaatttatc tcgtgtaatg gctgcttcca atgcactttg
      721 tttgatgatt tcttttgtgt tttcagggaa ataacgtaca atttcttgct ttggagggaa
      781 attttttgct tcttggtttt cccgaataag tttcatcaat ttctcaggct ccatcttacc
      841 aactacaaat aaaaccatat tgcttggctg ataaaacgtc cgatagcacg tatacaagtc
      901 ttgagctgtg attttgtcga tactttcaac cgtgcccgca atatcgatat gcaatgggtg
      961 tgtaggatac aagttattta gtatgccaaa aaacatccgc cagttagggt cgtcttcata
     1021 catttggatt tcctgaccga tgattccttt ttccttgttt acagtttctt ctgtgaagta
     1081 aggtgcttgt acaaaatcga tcagagtcgt cagattcttt tctacctgat ccgtcgtaga
     1141 aaacagatag ctggttttcg taaaacttgt gaaagcattg gcggaagctc cttgtttccc
     1201 aaataattga aagacatcac catcttcttt ttcaaacaac ttatgttcta aaaaatgggc
     1261 aattccatct ggaactttca ccttttcttt ttcaccatag ggaataaatt cgttatcgat
     1321 cgaaccataa ttcgtactga acaaaccata tgtcttgtga tagtcatttt taggcaacaa
     1381 atacacggtc aaaccgtttg gcagtacttc atgataaagt gtttcattga tttgctcata
     1441 ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa gattgcctgc aattcgattt
     1501 gctcagctac ttgttgtatc tctttgatcg ttacgctctc catacgtttc aaccattctt
     1561 catcagataa ttttgtttcc ggcagccagc tgtccaaaaa agaagcttcg attgccgctt
     1621 gcggagagtc tagagaaagc agatattggt ttctcagcat tgcttttgtc tgagacagtt
     1681 ccaactctgt tatttcacca tttcgcaaac tttctaattg ctcgtgaatc agatgaagta
     1741 cttgatttcg gttcttttca tcgataccgg tttggacact catgaatcct cggaacgtat
     1801 ccacgctact agaggcataa taagctagac tttctttctc tctgacattc atgaaaagtt
     1861 tagagtgagg aaaaccgcca aataaaccgt taaagaccat tagcgcaaaa cgttctggtt
     1921 catcgtaata cacatttgtc tgataagcta ggttcagctt tgcttgaacg attggctcac
     1981 gtacttgtcg ttccttcacg atattcactt gaggttgggt ataaaacaga tctggataaa
     2041 ctgtttcccg cgactcaaat ggtaattgcc caactgcttc agctgctttg tccggatcaa
     2101 catcgccgac aacaaaaatg tcgatttgat catttgccat catttcttga tagtaggacg
     2161 ctagcgaact agcagtgatc ttgtccaaat cttcgactac gcccaaccct gggatctttt
     2221 gttccgggct gttttcaaaa taaagttctt gcaaggcata ggaagcaaaa gtttgtttat
     2281 cttccttcaa actttccaaa tatagacgca gattgttctt ttccaagtca aacgtcaact
     2341 gatcaaactg atgattttta atatttggat aaaataaaac ctcttttaaa aaatctaccg
     2401 cttgagaaaa aaggtttgga tcattgacat attcaccatt cacaaaactg atccctgcgt
     2461 ttagccaatg taaatttcct ttttttctaa ctgataatcc aaagcttgcg ccataaagct
     2521 ctgcaagttg cgcacttaac tttgtctgat ccgaataatt caaactattt gtttccaaca
     2581 tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga
     2641 taaaaatccg gatcgttttg tattgttttg ttggtaagac atgaagattc acgccttctg
     2701 ccaattgata caccatattt atatgctcct tttcacagtt gattgcccgc ttactctttt
     2761 aatgggtcaa cctgcctttt ttacttattc ctctgcttga aatttataag ttcaggcaat
     2821 tcgccgtttt ttctaaatga tgttacactt tatagtatag catagatgtt ttttatcaaa
     2881 agaaacagac ggctaaaagc gtatattgtt tttttatttt ttatttttcg atatactaaa
     2941 gacaaataaa agaagggagg gtcattcatg atcaaagaat tcaaagagtt catcatgcgt
     3001 ggagatgtct tggatcttgc tgtcggcgtt gttattggta gtgcgtttac aagcatcgta
     3061 aaccaagtgg tgcaaggctt gatcacacca ctaataggtt ggtttgtcgc aatgatcaca
     3121 ggtacttctg atttagaagg agcactttca attcttgact ggtcgccgac aaaaggcgtc
     3181 acatttgcgt ttggtaacgt tattagtgct atcattacat ttctgatcac tggttttgtc
     3241 ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa
     3301 gtggaagtgg aagaaactcc tactgcagaa gattatctta gtgacatacg cgatcttctt
     3361 gccaaacaag tggcagaaga caatacacat ccagtagagc aaacaaatct ttccgatact
     3421 tctaaataat aaagaatcta aaaaaaagaa agccgatttt cagtgtcggc tttctttttt
     3481 tagattctta acttgctact tgttcctttt tcatgtcgat atgtggaata tcatcttcca
     3541 aatacacatc tgagattgcc ttgaatccaa acgactcata aaaagcttgt aggtattctt
     3601 gtgcgccgat cacgattggt ttttctggaa aatttttctg gatcatgtta atcgtttctt
     3661 gaacaatctt tcttcctaaa ccatctttac ggttgtcctg atgaacaagg acacgtccaa
     3721 aatggatact ctccgcttct tcatatactc gcgtgtaggc aaggatcttc ccatcagtat
     3781 cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc aacttcttga taaggacact
     3841 gttgttccac aacaaaaact gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg
     3901 acaattcttc aaatttcttt acctgatatt ccactaagca aacgccccct gcttttttat
     3961 tcttagcata ccgattcgca cacaaaaaag caaacgaacc ttacgaatct caggctcgtt
     4021 tgctttttaa actgcttcag ttaaaaattc gatctcaact gtccgagtaa gtacatctga
     4081 atagctgtaa gatactcgtt caaaagaatt ttcttcagga tccaaatcaa caacgaatac
     4141 tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg
     4201 agcaaccaac atgattgggc tgccgatacg accttctaaa tctttcttga ttgaagctaa
     4261 agttgttggc atacagttca cctctactat caactatacc acatattttt aaaattttca
     4321 agtttaccat aaaaaaaaaa gaaatgcaat aaaaataaat aagaagctca ttaagatttt
     4381 tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt ttactctccc
     4441 gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt ctacaaaatc
     4501 aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact ggatttcgca
     4561 attttcgatt gcttctttta ataaaagatg ctgaattgct aaaggttctt gcaatgtgat
     4621 ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga cttcatcttt
     4681 ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt gattgcttat
     4741 aacgctttta taataacttc caaagctaac acctttgctt ttttcgtaag ttctcaatgc
     4801 ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat ttcttaaata
     4861 ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg cagcaaatgc
     4921 ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat aattcctcca
     4981 taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt agaacgatat
     5041 gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt aactgctctt
     5101 cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt tcttgggcta
     5161 ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt cgtaatgctc
     5221 cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc acttgtgtca
     5281 aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt tcgtcttctt
     5341 tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc ggaactagtt
     5401 gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt gataaacgat
     5461 gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg acaagttccg
     5521 gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc atggtctact
     5581 ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca ctggcattta
     5641 agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct tttttcaatc
     5701 ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct ttggcattcc
     5761 atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct gtttctttga
     5821 gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt tcgactgccc
     5881 cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt ggtatgatca
     5941 caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg tgtggatctt
     6001 cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt cgttcaatca
     6061 gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct tgatgcacgc
     6121 cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc acagcttgtt
     6181 cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct tgaagaaaga
     6241 gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca aagacgaaat
     6301 tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca gtttgctttt
     6361 tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga tgtttgtttg
     6421 ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat caattcttcc
     6481 atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa ccctgttgcg
     6541 acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc atttcttcct
     6601 ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt cttccagcat ttgctgaatc
     6661 aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg cacgatgcag cttattgggt
     6721 tttgtttctc cttgatcaat caggtggtca cgaacataga tttcgtaaat cgcatctcca
     6781 acataagcca aggctaaacc gttcaattgt gtgtaatctc tcatgcttct cttctccatc
     6841 gggttccttg cggtgtatct tctaataaga tccctttttc tttcaacaaa tcacgaattt
     6901 cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg atcaattgtt
     6961 caatctcttc atctaataac tcttctgctg taaataaaat accgaagatc gaaagccatt
     7021 gcgagaataa agcttctcct tgtgccaaga cagcggcaga aactttcggc tggtctagat
     7081 aagtgttcag ccacttagca agctcataga ccactgtgat cccatttgct gcattaaaat
     7141 catcgtccat ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg attttatccg
     7201 catcatctgc caaattctct gaggcagttt cttggcggaa tttcaaattg tcaaacgtat
     7261 tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg tagcggatcg
     7321 gacgacggta ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca acgtttttga
     7381 tcatttcatg tacagtgatg aaattcccta gagatttact cattttttca tcgtcttcgc
     7441 caatcgttac atagccattg tgcatccagt aattagcgaa ttttttccct gttttcgctt
     7501 cactttgtgc gatttcattt tcatgatgag gaaattctaa atcttgcccg ccaccatgga
     7561 tatcgatcgt ttctcctaaa tgttttgttg ccataacgga acattcaata tgccaaccgg
     7621 gacgtccttt gccccatggt gaatcccaag aaatctcgcc aggttttgct tgtttccata
     7681 aagcgaaatc taatgggtct tctttttgct gctgttcttc ccctgtacgc tgacttgctc
     7741 cgacttccag ttcatcgatg gattggtcac ttaatttccc ataattttca aatttacggg
     7801 tacggtaata cacatcacct gaagattcat aagcatagcc tttttcaacc aacacttgaa
     7861 taaatgctaa aatatctggc atatgatcca tgactcttgg atgcagtgta gcaggttcga
     7921 cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa acgatcagca acttctggtg
     7981 ctgtaatacc taattcgttt gctgtacgga tgattttatc gtctacatcc gtgaaattcg
     8041 atacgaaatc gactttatag cctcgatatt caaaatagcg tcgaattgta tcgaaagcaa
     8101 tggtgctacg agcatttccg atatggatat aattatacac agtcggtccg cagacataca
     8161 taccgacatg tcccttttgt atagggatga attcttcttt ttttctggtc aatgtattgt
     8221 agatttttat cattctgatc tcctcacttt ttctcctttg attcgaacga cttttgctgg
     8281 tactcctacc gctgtagcgt cgtctggaac atctttcaac acaacggcac ctgcaccgat
     8341 tttggcgttt tttccgatcg ttaccggtcc taatatctgc gcattcgcag agagcatggc
     8401 tccttgttta acagttgggt gtcttttacc cgtttctttt cctgtgccgc ctaatgttac
     8461 gccatggaat aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga cgattcccat
     8521 tccatggtcg atgaacacgc cagttccaat cgttgctcct ggatggatct caacaccagt
     8581 taggaaacgc cagaattgag cattgatctt cgccaataaa aagagccggt gctgatataa
     8641 aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca aaacgatctc
     8701 agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc gggtaaacca
     8761 actcatgact ttccactcct ttcgtaagaa ttttatgtag gaaatcatct tcttataaag
     8821 aaaagaagcg ccttttaaat aagtttcctt atcaaaagac gctttccgcg tggttccact
     8881 tttcttcata atcaatgatt atctctgaca gctaacgcct gttatacgtt cttggctact
     8941 ttgtttccta taaaacagtt tttcctgttt tctgtgtttc gccaagacca ctcccagagg
     9001 catttcggca accattcatt gataccttgc accacccggt atctctcttg taatgatcag
     9061 ctgcttactt ttttctgttc atcgtgttta gttcagtact tgatttaaat gtgctaatga
     9121 tttttcacga ccaagcaatt cgatcgtttc aggcaattct ggaccatgca tttgtcctga
     9181 tacagctaca cggattggca tgaaaagatt ttttcctttt acgccagttt ctttttgaac
     9241 agctttgatg gcagctttga cagttggagc atctactact tccatttgtt ctagctgtgc
     9301 tttgaatgcg tggagaacag taggaactgt ttcaccagca agtacttctt ttgctgcttc
     9361 attcaataca ggatgttcat tgaaaaatag cgtagacaga tcaacgattt ctgcagcata
     9421 gctcatttga ggttgataca ggctaacgat cttcttcacc cattcgattg tcttttcatc
     9481 tgggttttct gctacacgtc catcagcgat tagataaggc agacacatgt cagtaaatgc
     9541 atctagatcc attgctttga tgtaatggtt gtttacccat tgcagttttt tcgcatcgaa
     9601 agctgcgggt gatttactta ggcgttttgg atcgaacatt ttgatcaatt cttggtgttc
     9661 gaagatttcg tcttcgccca caggtgacca gcccaataat gcaataaagt tgaacattgc
     9721 atctggtaga tagcctaatt cgcgatattg ttcgataaat tgcaagattg attcgtcacg
     9781 tttgcttagt tttttacctg tttcactgtt gatgatcaat gtcatatgtc cgaaggttgg
     9841 tggtgtccag ccaaatgctt catagatcat cagttgtttt ggtgtattgg caatatggtc
     9901 atctccacgt aaaacgtgag tgattttcat caaatgatca tcgaccgcaa cagcaaaatt
     9961 ataagttggc ataccatcgc gtttttggat gacaaagtcg ccaccgatat tatcggattc
    10021 gaacgaaata gcgcctttca ccatatctgt aaatgcgtat tctgtattgc gaggcacacg
    10081 gaaacgtaca acagattcta gaccttgtgc ttctttttct gcttgttctt caggtgtcaa
    10141 gttcgcacat gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg cttcccgttc
    10201 tgcttctaat tcttcttctg tgcaatagca tttgtatgct cgattgctag caagcagctg
    10261 atcaatcagc ggttgataga tttctttacg ttcagattga cggtaagggc cgtattctcc
    10321 aggattttca ggagattcat cccaattcat tcccaaccaa gcaaggtttt ccaactggct
    10381 tttttcgcca tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa taataaagtc
    10441 tccatcattg tggcgggcaa acaaataatt gaataatgca gttcttgcat ttccaatatg
    10501 caggtgccca gttggacttg gtgcatagcg tacgcgtact ttcgtcattt tcttcctctt
    10561 ctttcaaact agactttgac taacagcaaa aattccgtca gttacggctt ctcaaatatc
    10621 atttatttaa agccactcag cacaaatttt accttttcta aggtaaatag taaagccaac
    10681 tggttgaatt tccttaccag ttggctgaat ttctttttta ttttgattgt ttagtctttt
    10741 tctctgtctc ttttttattg tcgattcctc gtcctgcatg tgcaggttta gcaaagatca
    10801 tgcgtccagc agctgtttga agggcacttg ttacaacgac ttcgatacgt tcattcatgt
    10861 aatgctgtcc atcttctacg acaaccatcg tgccgtcatc aaggtaggct actccttgtt
    10921 ggcgttctgt cccggctttt actactagaa cattcatcgt ttcaccaggg atgacgactg
    10981 gttttacggc atttgccaac gcattgatat taagaacagg tacattttgg aattcagaga
    11041 ctttattcaa gttatagtca ttggttacaa tgacaccatc taaaagctta gccaatttga
    11101 tcaatttgct gtctacttca ctgatatctt caaagtctcc atcgtacatt tcgacagata
    11161 taccctcttc tttttgcaag gcatttagga tatctaatcc gcgacgacca cgcacacgtt
    11221 tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa ttagggatca
    11281 tcaaggttcc ttctaagaat ccagtctttg taatatcata aatccgaccg tcaatgatca
    11341 cgcttgtatc caagatttta tatttatgga aattttctcc tgattttcgt tctaacagtt
    11401 gactctcaac ttgactagct gtttcttgct cgattttttt gcttcgtgaa ccaaaaactt
    11461 tcttccattc ttcgattcgt gtcgttccca ttctcaatcc cagatagcct aagatgatca
    11521 tcagtaaaat cggcaataca ctattgacga atggaatttc catattgtaa agaggggtcg
    11581 agatcacaac acctaaagtc aatccgatga tcgcaccgat cgcaccgaag agaagataag
    11641 tcaagctgac ctcacttagt ttttgttcta cttgtttgat aatagctgag atatgtttgg
    11701 ccaacagcaa agataataaa aagaaaataa gtgcgcctaa caggctgttg gtaaatgtat
    11761 tattcagcca tccgtttgac tgttgaccga ttgcctgcca agctgagggt aaaaatgaaa
    11821 tacccaagct tgcgccgaca atgatcatca gcaaagtaat gacacgtttt tgcatcgttt
    11881 ttcctccgtt tcgacaatca tttattagtt agcggaacac acgcttcaac gtttcagcca
    11941 aagttgctac tccaactatc tcgattcctt ttggcgcttc ccagttacct aagttgtttt
    12001 taggtaggta tacttttgtg aagccgagtt tttggacttc tttgactcgt tgttcaatat
    12061 gactgacacg tctgatttct ccagtcagtc caatttcacc gatgaaacat tccgttggct
    12121 gtgttccttt ttctttatag cttgaagcaa tactgacagc gatagctaaa tcaatcgctg
    12181 gttcatttaa ttttacccca cctgcagctt ttaagtaagc atcttgattc tgaagaagca
    12241 acccagctct tttttccagc actgccatga tcagagatac ccgattgaaa tcaagacctg
    12301 ttgttgttcg tttggcattt ccgaacatag taggtgtgac taatgcttgg atctctacca
    12361 aaattggacg tgtcccttcc attgccacaa cgatagcaga gccagttgca ccgtctaggc
    12421 gttcttctaa gaaaatctgt gaaggattag cgacttcttc caatccatgc tcatgcattt
    12481 caaaaatccc aatctcattc gttgagccaa atcggttttt tactgctcgc aaaatccgaa
    12541 aactatgatg ttgttcacct tcaaaatata acaccgtatc caccatatgt tctaacattc
    12601 ttgggccggc gatcgagcct tctttggtaa catgaccgac gataaaaatg gctatcccat
    12661 ttgttttagc ggttttgagt agttctgcag tggtttctct tacttggctg acacttcctg
    12721 ccacacttgt gatgtctggc tgcgtcatcg tttggatgga atcaatgata acataatcag
    12781 gggagatatg ttcgatggca cgactgattt cgttcatatc tgtttcagcg tatagataaa
    12841 attctgtgtt gatacttccg aggcgttccg cgcgtaattt tatctgctct gcgctttctt
    12901 cgccggatac atataagact tttccgccta tggctgctaa ttgctgagaa acttgtaaca
    12961 gcaaagtgga tttcccgata cctggatcgc caccaatcaa gaccatagac cctggcacca
    13021 cacctccgcc taacacacgg ttcagttctt ctaattttgt tttgatccga ggttcttttt
    13081 tagggacgac gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa cttgtccgag
    13141 ttctgcggtc cgttgtatct tgttcgattt cttcgaccat cgtattccat tttccgcaat
    13201 ttgggcagcg tcccaagaac ttaggcgaca catacccgca attttggcaa acaaattgta
    13261 cttttgattt ttttgccatt gtaccgcctc cttttatcct aatcatgaga cattaaatca
    13321 agcttaagta tctttaacgt tttatttact ggaagaacca aatccgcctt gacgttgagc
    13381 agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag atcccttggc cgatacgctc
    13441 gccttttttg atcactacat ccttgaaacc aaagttggtg aattggaaca tgatatggcc
    13501 ttcatttgcc tcattattgt aataatcact gtcaatcaca cctactccgt ttgtcaacac
    13561 taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt catcttctcc
    13621 catgtaagct ttgattccag taggcacgag ggttggtttc aattgttttt cattttctga
    13681 tacatctttt ttcaatccat ctgcgactgt ttccaacccc tttttccagc ctgctttcca
    13741 gatacttgga atcacgacct cttctgctgc ttcaaaatca tagccagcag catgatgtgt
    13801 agcgcgctgc ggtagattga tattttgatc tttatattta gaaataactt caaatcctct
    13861 ttttctcacc ggttttcctc cctaaataaa ctactcctat tctaacatat ccgaggctgt
    13921 aaaagacttt ctttacaaat tcttcaagaa attctgctat cttaaagtaa accattaatt
    13981 cgttcttttt ccaaacgact gcaagttgta caaaaaaact attttttaca tgactttact
    14041 cgtcaaaaca agtagaatag aacgaaatta atgagtggat atttgattga ggtgtcacat
    14101 gcgtaaagaa attaaaaatg caaaacgaat tattgtcaaa gtaggaacaa gtacattgat
    14161 ttatcccaat ggaaatatca atcttgcggc aatcgatcag ttagcttttg tcctttctga
    14221 tcttagaaat cggggaaagg aagtgatctt agtctcatca ggagctgtag gcgttggtat
    14281 gcataaatta catttgtcag aacgtccgac tttgattcct gaacagcaag ctgcggcggc
    14341 tgttggtcaa gccgaactga tgaatatcta taatcaaaga ttcagtgtat ataatcaaca
    14401 gacggcacag atcctcgtga caagagacgt ggtagattat cctgaaagtc gtaaaaatgt
    14461 gaggaataca ttggaacagc ttttgattat ggacacgatc cctatcgtga acgaaaacga
    14521 tactgtagca gtggatgaat tagatcatct gacaaaattt ggcgataatg atcagctgtc
    14581 agccatcatg gcaaaacttg tcgcggcaga ccttttgatc gtcctttctg atatcgacgg
    14641 gttttattca gataatccga ctacaaatcc cgaggctcag atgtatcgct atatcgataa
    14701 aatcgatgaa aagattctgg cacaagcagg tggtgctggt agcacatttg gaacaggcgg
    14761 tatgcagagt aaattaaaag ccgcttctag gatatttgaa gcaaacagcg caatgatttt
    14821 agcaaatgga aaaaatccgg caattatttt tgatattctt gcaggaaaag aagtaggtac
    14881 tttatttaag gaggacttga aatgactgac ttgatgcaat taggacaaca ggcaaaagat
    14941 agcgcgcaac aactagcact tatgcctaca aaccgcaaaa atgagctact tgttcaaatg
    15001 gcacaaacca tcaaaaagaa tcaacaagcg atcattgaag caaatcataa agacttagaa
    15061 aaagcagtcg aaaacaatat cagtgaaacg atgcaagacc gcttgcagct gacacctgag
    15121 cgaatcaatg ccatggctac agagatcgaa aaaattgctt cgttagacga tcctattgga
    15181 aaagtagatg agatgtggac gaacaccgac ggattgagaa tcggcaaaaa gcgcgtgcca
    15241 ctaggcgtga tcggcatcat ttacgaatct cgtccaaatg tcacgacaga tgcagctagt
    15301 ctagcattca aatcagggaa tgccgttatc cttcgcggtg gcaaagaagc attcttctcg
    15361 aatcaattat tagtccaatt attgcagcaa gtactggtca gtgctggcga atctccttat
    15421 gccatccagt ttgtcgatga tacttctcat gaaactgcag gacagttgat gcgattgaca
    15481 gaatacttag atgtgctgat tcctcgaggc ggagcaaaat taatccaacg agtcaaagaa
    15541 caggcaacgg tacctatcat tgaaacaggt acaggaaata atcacgtata tatcgataaa
    15601 gaagcacagt tatctatggc agtgaacatc atcgtcaatg caaaagcttc ccgtccatct
    15661 gtctgtaacg cggcagaaac attgctgatc cattctgaga tcgcaccttt tttcttgcca
    15721 gctattgaaa aagaactggt agaacacggg gtctctttac gtgcggatgc tcgtgcgtta
    15781 gaatatctag agacggctgc tttagcagaa gacacagatt gggatactga atatctagat
    15841 tatatccttg ctgtcaaagt ggtcgattct ttggatgaag caattgcaca tatcaatcgg
    15901 cataatacta aacattcaga aacgatcgtg acagatagtt atgcggctag tcaacgtttc
    15961 ttaaatgaag tggacgcggc ggctgtctat gtcaatgcct ccactcgttt tacagacgga
    16021 tcggtatttg gttttggtgc agagattggt atctctactc aaaagttgca tgcccgtggt
    16081 ccaatggggt taaatgaatt aacttccaca aaatatatca tttatggcga tggacaaatc
    16141 cgcgaataat gtttcgtgtg aaacattatt cccgcctttt gccttttttc tgtaaaggtt
    16201 ctatccaact agaaaggtgt caatatgttt gaaattttaa tgattttttt tatttacctt
    16261 atttctctga atatcgctgc ttttctggga gtcggtattt tatccctttt ctttcaattc
    16321 aaaaaacgaa gcatagggag tcaacgggaa aaatggtcgc agtattttga caaaatcggg
    16381 caaaaagggc tggttacacg cttgcacatt agttacatgg ttgcattatg cttattagcg
    16441 acaatcaatt actattcttt tttcgatcat tcaatcgctt atacgattac gctactgatt
    16501 gctgggattt ttcacttatc ttataaatat cagctgaata aaaaccactt gaatcggact
    16561 ttccgctaaa aaatatccgt acgataagta gagtgccttg aagatcggca aaaaactgcc
    16621 ggacttttta gaggctgtga caaagatttt gtcacagcct ctttttccat ataaacggcg
    16681 ttcgcctacc agctgcctgg taaatctgtt tcctcttcgt taagaagcga aaatccgtca
    16741 aatgtttcgc catcataata acgaagcagc tcatccatgg acaagttcat cgttttttct
    16801 ttttcttctt ccgctgattc a
//
LOCUS       DASJON010000045        16494 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJON010000045 DASJON010000000
VERSION     DASJON010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.045"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..1336)
                     /locus_tag="U3989_001096"
     CDS             complement(104..1336)
                     /locus_tag="U3989_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN1714557.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="U3989_001097"
     CDS             1591..2160
                     /locus_tag="U3989_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1714558.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="U3989_001098"
     CDS             complement(2338..2778)
                     /locus_tag="U3989_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN1714559.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="U3989_001099"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="U3989_001099"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN1714560.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="U3989_001100"
     CDS             3732..4655
                     /locus_tag="U3989_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN1714561.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="U3989_001101"
     CDS             4731..5870
                     /locus_tag="U3989_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN1714562.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="U3989_001102"
     CDS             5863..6663
                     /locus_tag="U3989_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1714563.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="U3989_001103"
     CDS             6663..7490
                     /locus_tag="U3989_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1714564.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="U3989_001104"
     CDS             7468..8202
                     /locus_tag="U3989_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1714565.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="U3989_001105"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="U3989_001105"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN1714566.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="U3989_001106"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="U3989_001106"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN1714567.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="U3989_001107"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="U3989_001107"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN1714568.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="U3989_001108"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="U3989_001108"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN1714569.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="U3989_001109"
     CDS             11787..13820
                     /locus_tag="U3989_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714570.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="U3989_001110"
     CDS             13864..15144
                     /locus_tag="U3989_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN1714571.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="U3989_001111"
     CDS             15354..16160
                     /locus_tag="U3989_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714572.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..>16494
                     /locus_tag="U3989_001112"
     CDS             16172..>16494
                     /locus_tag="U3989_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1714573.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGY"
BASE COUNT         5027 a         2843 c         3436 g         5188 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta ttagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atga
//
LOCUS       DASJON010000046        16260 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJON010000046 DASJON010000000
VERSION     DASJON010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.046"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..273)
                     /locus_tag="U3989_001113"
     CDS             complement(<1..273)
                     /locus_tag="U3989_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HEN1714574.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEK"
     gene            complement(387..557)
                     /locus_tag="U3989_001114"
     CDS             complement(387..557)
                     /locus_tag="U3989_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714575.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(554..1231)
                     /locus_tag="U3989_001115"
     CDS             complement(554..1231)
                     /locus_tag="U3989_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1714576.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(1322..2227)
                     /locus_tag="U3989_001116"
     CDS             complement(1322..2227)
                     /locus_tag="U3989_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1714577.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            2402..3856
                     /locus_tag="U3989_001117"
     CDS             2402..3856
                     /locus_tag="U3989_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1714578.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            3872..4903
                     /locus_tag="U3989_001118"
     CDS             3872..4903
                     /locus_tag="U3989_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1714579.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            4910..6097
                     /locus_tag="U3989_001119"
     CDS             4910..6097
                     /locus_tag="U3989_001119"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN1714580.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            6253..7635
                     /locus_tag="U3989_001120"
     CDS             6253..7635
                     /locus_tag="U3989_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1714581.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            7762..7992
                     /locus_tag="U3989_001121"
     CDS             7762..7992
                     /locus_tag="U3989_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714582.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(8192..8860)
                     /locus_tag="U3989_001122"
     CDS             complement(8192..8860)
                     /locus_tag="U3989_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN1714583.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(9049..10475)
                     /locus_tag="U3989_001123"
     CDS             complement(join(9049..9902,9902..10475))
                     /locus_tag="U3989_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1714584.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQFRKTTVTVV"
     gene            10718..11173
                     /gene="tnpA"
                     /locus_tag="U3989_001124"
     CDS             10718..11173
                     /gene="tnpA"
                     /locus_tag="U3989_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN1714585.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            11438..11536
                     /locus_tag="U3989_001125"
                     /pseudo
     CDS             11438..11536
                     /locus_tag="U3989_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12219..13037)
                     /locus_tag="U3989_001126"
     CDS             complement(12219..13037)
                     /locus_tag="U3989_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1714586.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            13198..13887
                     /locus_tag="U3989_001127"
     CDS             13198..13887
                     /locus_tag="U3989_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1714587.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            13901..15403
                     /locus_tag="U3989_001128"
     CDS             13901..15403
                     /locus_tag="U3989_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1714588.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            15416..15892
                     /locus_tag="U3989_001129"
     CDS             15416..15892
                     /locus_tag="U3989_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1714589.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(15989..16099)
                     /locus_tag="U3989_001130"
                     /pseudo
     CDS             complement(15989..16099)
                     /locus_tag="U3989_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5045 a         2930 c         3054 g         5231 t
ORIGIN      
        1 tttttcttca gaagtttgtg tctcttttgt tttagttgat gactcacttg tttgtttcgt
       61 ctctgttttc tttgtcgttg tttccgttat ggtcgtactt ttcgcttctg acgaattttt
      121 tccatgaaaa tatagacccg caactcctcc tgctatgatc aaaaacacgg caactgctgc
      181 cccaatcatg atcatccgct tacgagctgc ctgtttttgc tgtcttcttc gggtcatccg
      241 aacattttct ccccactcat tttcatcata catttttttc accaccaatt tttatgtata
      301 atagcgttat caaacgcttc atattcttat tgtagcaaat aattgtcgct gtgcacattt
      361 ttatctttgt tatttataag aaaatttcac cagttgagat gattccaaga ttgtccgtcg
      421 atttctcctt gcagctgcat tgttcgttcg tctatctcag ccatcagttt ctttatttca
      481 tataagagcg cacggtcttg atattctgat ttttcgctta attcatctaa ttgttggatc
      541 agccaatcat ttgtcataat gaagttcctt tcatttcttg cagtgtaaag gtcaagtctt
      601 cgatctgctc ttttgcctca gtcagtgctt ctacgatata tgcttgtttt tcagattctt
      661 cttcctgcat tttctgattt tcctgtactc tttgaataaa ccagtcaatc cgctgagtca
      721 gcgatggttt gccttccaat gagagatact gctgataagc aattagcttc tcccaacgtt
      781 tttcctggga taaatacata aattgtgcta aaacgaatgg cggaagatag gtcatatgga
      841 atttatttgc gattcgctga tacggcctca aaaattcgga aatcgtatat tcttcttttc
      901 ctccagcttg gtattctttt tccgccacac ctgttgtcaa tacgattcct agttctttcc
      961 cttttaatac atgtgctgct cgttccaaga cattatcttg ccaaattttt aaatgagcag
     1021 gagaactata ccagtaaaac ggaaactgga aaatgatccg atcatgctgg gccaagagct
     1081 gtagttcagt ctctatatct atttttccgt ctggataact tgattccaaa tgatgccaag
     1141 tagctggtaa accagcagaa gcttctttga aaaattgctg atttaaagat tcttcgatcg
     1201 ttggatggct aatgatgata agtgtacgca tcccttttcc tcctttttag tcttcgcttt
     1261 acagtgtagc ataaccgcca caagtttctt ttgccgatgc ttgtcatttt aaccagacta
     1321 atcaggttgt ccaagaaact gcggtttgtt tttccaactg ttttttcaag gtatccaaag
     1381 caatattctt aaaattttcg tttttcatac gtttacgata gttcgtagcc gtttctccgg
     1441 tactcttttt gaacattcgg ctaaatacga ctagactatt gaaccctact tgataggcaa
     1501 tactttccac ggattcatcc gtcagagaaa gaagaaacgc ccctcgattg atccgtaccc
     1561 cgatcaaata ttccttgata ctggtaccca tatgattttt gaatactcgc gacaaatggc
     1621 tccgattgac cgctaaatgc tgggcgattt cctcaacaga caatttttca gcataatgtg
     1681 cctcgatgta tttgactgtt tcaacagcta aagaggaatg aaccttttgt acaccaccgc
     1741 tgacctttcc gccatctttt aaaaggactg ataaaaaacg gtaggttaat gctgttaact
     1801 gtaattcatt ttccaaggtt tcttccgtat ctttcatgca ttctaaaata atgtcgatat
     1861 atttttgtat gtcagaagag accatcgtgt attggtcttc tctgaaaagc gactgccgaa
     1921 tgatttcttc tgcagcactc ccaccaaatg aaagccagca atacatccac ggatcttctc
     1981 catctgataa gtaaaacgtc gagtctcctg gacgaatcaa gaacagatca ccttttttca
     2041 attggaattg ttgatttttc acatgataag atcctttgcc ttccatcacg acatgcagga
     2101 tgtaatcatt gcgaatagca ggaccaaaag aatgaaacgg catcgtttta gagaaaccac
     2161 aaaaatcaaa atacaattct ttttctactt gttgatgttc cttttttaaa aaaatcgatt
     2221 gctccacgtc tatccctcct tactccttta gtataatgca acaaaaagtt aaataaaagt
     2281 cacagaaata tattaaaaag gaagcgattt cttgttaagt ttaagtcatt aaaagatctt
     2341 tgttattcgt tgtaaccggt ttacttagta ttatatattg aacaggagag gaatacagat
     2401 tatgaaaagc aaaatgatcc aacgtgtgtc ctacgcttta ggcgcttttg gacacgacac
     2461 attctatgtt acattaagca cttattttat ggtattcgtg acaagccaga tgttcgcagg
     2521 aacagataag gcaacgaatg caaaaatgat tgctacagtt acaagcttag ttgtaggtat
     2581 ccgcttagtt gaaattattt ttgatccttt gattggtggc atcatcgata atactcgtac
     2641 aaaatacggt aaattcaaac catggttagt tatcggcggc gttgttagtt ccgtactctt
     2701 ggtagcaatc tttaccaatt tctttggctt agctactcat aatacgactt tatttatcat
     2761 attatttgca atcgttttca tcttattgga tagtttttat tcatttaagg atatcgcttt
     2821 ttggtcaatg attccagctt tgagtacgga aacaaaggaa cgtgaaaaat ttgctacgtt
     2881 tgctcgtttc ggctcttctc taggtgcaaa tggtactact ctagtcgttg tccctatcgt
     2941 tactttcttt acctatcttg ttactcataa acatattgaa ggcgcttcag gctggttctg
     3001 gttcggtgtg attgctgctg ttatttcagg cggtactgcg attatcactg ccttgggcac
     3061 aaaagaagtc gatactgcga tccgtcagca aaatgaaaaa gctgggatca aagatgtgtt
     3121 caaagcagtt gctcaaaatg atcagttgat gtggcttgcc cttgcttata tcgcttatgc
     3181 gattgccaat gcagcaacaa ctgccgtatt attctacttc ttcaaatatg tacttggcca
     3241 accaactgaa ttttggatcg ttggtctagt tgctgcctta cttggtttag tagctgttcc
     3301 actattccca atgcttacac gcgtcatctc tcgtcgctac gtttaccttg gagggatcgc
     3361 aatgatgatt tcttcttact ttttctttac gatcgcaggt tcaagtttgc caatcgttat
     3421 catcgggcta gtattgttct acttccctca acaattgatt ttcttatccg ctttgatgac
     3481 gatcacagac tctgttgaat atggtcaatg gaaaaatggt atccgtaacg aagctgttac
     3541 tctatcaatt cgccctttac tagataaaat cgctggtgct ttgtctaacg ggatcgtagg
     3601 gtttgtcgct atcgcttctg ggatgactgg taatgctact gctgctgata tcactgctca
     3661 tggcgtaacg actttcaaag cttatgcatt ctatgctcct gctgctttga tgattttctc
     3721 cgctattatt tttgcttgga agattacatt gacagagaaa aaacatgcac aaatcgttga
     3781 gaaattggaa aacaaattgg ctccagctga tacaatggaa gatgaacttc acgatgcagt
     3841 agatccttcc cattaagaaa ggatcttgaa aatgaagatt acagaaaaag attttggcca
     3901 aggttatcac ttgataacct tggagaacaa aaacaagcta gcactttcta tctctgattt
     3961 aggtgctaga atcgtcagtt taaaatcaaa tgatcgagaa ttagttttgg gatttgacac
     4021 cgctgaagaa tatatcgaaa aagatccgta tatcggtgct tctatcggtc gcaccgcagg
     4081 gagaatcgaa aatggccgct tttctctcaa tggaaaaact taccaattag ccacagatcc
     4141 aaaaactgga cataatctgc atggtggtgc tcctggattt gaattaaaaa aatggtctta
     4201 tgtcattttg aatggcgaaa atgaagcttc tgtcattttt actactacaa gtcccgacgg
     4261 agaacatggt tttccaggta caatggatgt ggaaatccgc tatactttga caaaagataa
     4321 tatctggcga gtgacctctc gcggcaccag tgaccaagat actttattca atccgacaaa
     4381 tcatgtttac ttcaacttga cgggagatgc ttctcagtca atcgatcaac atgagctttg
     4441 gctcaatagc gaagcttatg ctcctttgcg aacagatagt attcctatag gggtaaaaga
     4501 aaatgctgca ggaagtgctt tcgattttca aatacctaaa aaattagcgt ccgtttttgc
     4561 tagtgatttg gatcaaaaaa atcttgttga cggaatcgat catccatttt tcttaaaaga
     4621 aaccggcttg ggtaaagaag ccgctcgctt gactagcccc gataaaagaa tccaagtaga
     4681 tattgctaca gatgcttcta gtgtggttat tttcactgca aatttcggca cagaaacacc
     4741 agaaatgcgc aaccgaaaac tggcacatca cggtggcatc acatttgaga ctcaaacagc
     4801 tccaggtgca gagcgcttct cagcatttgg ttcgattcat ttaaaagccg gatcagtatt
     4861 tgaaacagta acagaattca aaatcaaaac aagaaaggaa taactcatca tggaaatcaa
     4921 aagaagctta aacacaaaat ttgccgaaac attcggacct aaaaaaacta cacaatactt
     4981 ctcaccagga cgcatcaatt tgattggtga acacacagat tacaatggag gccatgtctt
     5041 ccccgcttcc atcacttatg gaacgcaagg tgtagcagca cctcgagacg ataataaaat
     5101 attagtttat tccacaaact ttgacgatat cggtgtcatt agttttactt tagatgagct
     5161 agaatatgat aaaaaagcaa actgggcaaa ttacgtaaaa ggaatggtcc ttaaattaaa
     5221 agaagctgga cactcaatcg atcatgggtt tgagttatta gtggaaggaa caatccctaa
     5281 tggtgcagga ctttccagta gcgcttcatt agaattatta gtaggtgttg tattagaaga
     5341 tctgtttgat ttaaaaacag atcgcttgga actagtccaa acaggtaaaa aagtagaaaa
     5401 tgaattcatc ggtgtcaatt caggtatcat ggatcagttt gccatcggct tcggtgagct
     5461 agatcatgca atcttattag atacaaatac gctgaaatac gaaatggttc cagtgaaact
     5521 agatggttat gcaatcgtca tcatgaatac gaataagcgg cgggaattgg cagattcaaa
     5581 atacaatgaa cgccgtagcg aatgcgaaga agccttggca cgtttgcaaa aaactttgcc
     5641 tatctcttct ttaggagact tagatgaaga agaatttgaa tcaactattg accaaatcgg
     5701 cgatgataca ttgatacgca gagctcgcca tgccgtttct gaaaatcagc ggactttgaa
     5761 ggcaaaagct gaattagaag ctgggaactt agaagcattt ggtcaactgt taaatgattc
     5821 tcaccactct cttcgctatg attatgaagt aactgggatc gaattagata cactagtaga
     5881 tgctgctcaa aaacaagaag gtgtcttagg tgcacgaatg acaggagctg gatttggtgg
     5941 ctgtgcgatt gctcttgtca aagaagaaaa tatacctgcc ttcaaaaaca atgtctatga
     6001 tgaatacatg aaagttgtcg gctatgctcc ggaattttat gttgcacaca ttggaaatgg
     6061 gacaacaaag atcgatccag aagctgttca tgtttgataa aaaaatcaac gatctcagct
     6121 aaaaacaaat agataaaaat aacctctcag caatttccaa atgtgagcct tttgctcgta
     6181 tttgaaaatt gctgtttttt attgacacgg aaagagaacg tgtgttctaa ttgtgttgaa
     6241 gaggtgattg ttatgtgggc gaatcaaaat ccggtatttg tttatgaaaa agaaccttca
     6301 agagatattt tatgtattga ctgcaaatct ttttacgcct ccgtagagtg cgttgaaaag
     6361 gatcttgatc cccttacaac taaattagta gtcatgtcct atccgtccga ttcgacagaa
     6421 acacggggta gcggattaat cttggcttcc agtcctgcag caaaaaaagc ttacggcatc
     6481 accaatatca gccgtgccag agatcttccc ttcccctatc caagtgattt atatattgtt
     6541 gccccaagga tggcctatta tatggaaaag aacagagaaa tcaattctat ctataaaaaa
     6601 tatgcagacg aagcgaacca tcatgtctat tccgtcgatg aaagcttcct tgatgtaaca
     6661 aatagcatgg cactctttgg taaaacaaat gcgtacgaca tggctaaact gatccataaa
     6721 gatgtgtttg agcagaccgg aatctacacg actataggaa ttggcgataa cccgctactt
     6781 gctaaactag ccttagacaa tggctcaaaa gacggaaagc aaatgtttgc tgaatggcgt
     6841 tatgaagatg taccagaaaa gttatggaaa atctcagata tcactgactt ttggggaatc
     6901 ggcaaacgaa cagcaaaacg tctgaaacaa atgggtatac attctgttta tgatttagct
     6961 cacgcggatt attatcactt gaaaagcaag ctaggggtca tcggtactca gctttatgcc
     7021 catgcttggg gcatcgatcg aagttttttg ggacaagaat atacaccaaa atcaaaaagt
     7081 atcggaaata gtcaaatcct agatagagat tatacaagac gagaagaaat cgagatcgtt
     7141 atcaaagaaa tggccgacca agtcggcact cgcttgagac gatctgcagc aaaaacacaa
     7201 gtagtcagct tatgggtagg atactcttta ggctatcttg atccaaatgg caagaccggg
     7261 tttcaccagc aaatgaaaat cgaagcgaca aatgctagca gagatctagc agatgcttta
     7321 ttgaccattt tccaccagca ttaccactac caggatattc ggcgtgtagg cgtgaactgt
     7381 tccgacctcg tatacgcgac aggtttacag cttaaccttt ttgaagatcc tgaaaagcag
     7441 ataaatgaag cgaaagttga ttttgttgtc gatacgatta gaaaaaaata cggctttcaa
     7501 tcaatcatcc atgcgcatag tctcttgcaa ggaggacgcg ccatcgcacg tagttctctc
     7561 gttggcgggc atgcaggtgg tatgagtgga ttagaaggtg gaaaagatga gacggacagc
     7621 aaaaaaattc attgactata acgagtatcg agatcggcca tttggattaa aatgggggac
     7681 tgcttttgcg atggaagaac tagtcaacgg tatcaaaaaa atgaagaaga agcacgcaaa
     7741 cagcctcttc ctaaacaaca aatgacattg caagaaatcg atagtatact gacagaagcc
     7801 tttttatctc gtaaagcagt aatcattcag ctcaatttaa aagatgaatt tggaagactc
     7861 ttggatgagg tcgaagggca cttcattggt gaagcctatg aagattactt tatgattgac
     7921 catcagccga tattgtggga agacgtacgg tatattgagt taaaggaaac aaaaaaatgg
     7981 tttgtgacat gagaggttgt gacagaagtg atcaacttca aaaaataaga aaggattttt
     8041 cgaaaattgc ttcttaaatt tttgtggaat tttggcttat ttttcgaaga agcaacttct
     8101 ggaacaacgt atattagata tcagaggttg tgaaaaagtc gcttagtttt gagtaacaag
     8161 ccaaggcgct gatttcacaa cctctgtcta tctatccatc tattcggtaa gattcatgca
     8221 tgacgtcttc tttatttagt atgtagagcc ctttttcttc tgaccattca acataaatca
     8281 gctgttttac atctgcatag ccttctttct tcaagttctc cataagccat gcttcagtca
     8341 attgattgga ctgaagcact ttatgctgaa tttgcccttc atctacaagc aggatcgata
     8401 aagctttgtc ttcatctgat tttttcaata cactcatttg accggcattc tctaggataa
     8461 catgtttggc attttcaaga aaaaagcatt cttgctgtct taagattgct cgcaactgct
     8521 ccatctcgat atggttattt gtcaactctg tcatgttgat gacgccgtct ttgattagaa
     8581 cagatggctc gcctttgacc caacgattga ccttttcatt tttttggacg atacgttcga
     8641 taccataaat cattactgcc caaagcgcaa tggcaaatag gacatgccct acatggacat
     8701 tgtcatcata ggttgattct tctaagatcc cacctaaaac aagcgtatag actaaatcaa
     8761 acggtgtgat ttctgacatg gatttcttcc caagcaaacg taccactaat aataaaccga
     8821 tcaacccgaa caccagtttc attgtaattg aaaaaatcat tttttctccc cctaactatc
     8881 tgttaattaa attatatatg ctttctcacg aacacgcatg gaatatacaa ggaagaaatg
     8941 tatcctttta ctaaaaaaag gactgtgaca ctgtagtgac ccccaaaagt tagacttttt
     9001 tggagtggag aaatccactc ctttttttta tgctctaaaa tgatattttt acacaaccgt
     9061 tacggttgtt ttacgaaatt ggacgagact ttgccagttt aatttctgtt ttatccgttc
     9121 attattgtag taatagatat agctatcgat ggctgatttt aattcctcga aacagttata
     9181 aactttcccg tgaaaaatct cttgttttaa taagccaaaa aaattctcca taagtgaatt
     9241 atctaagcag ttccctttcc gagacatgct ttgaaagatt ttgtactcct tcaatttgtt
     9301 tctataagct ttcatttgat acgcccaacc ttgatctgag tgaaacgtac gacgataggg
     9361 acaatcgtta gtggtctgga tagcttcctc caaaccttcc attaccgcta atgcattggg
     9421 cttttcagag atacgatagg ataaaatctc actattgaac atatccataa aaggatctag
     9481 atacaatttc ttttgacgga taatgccttt ggcatccact tcatggtatt taaattcagt
     9541 tgtatccgtc gtgattttct gatggcagat actggtgtag aaacgacgat ggatccgatt
     9601 tttggctact gctccgactt gtcctcgata agaattgtag cgccgtgatt tacgtgtgta
     9661 tgtcgttact tttattccta gcttcttgat caggcgctga actttctttt tattgataga
     9721 gaaaccgcgg tttcttagtt cattggtcat ccgtaaacag ccataatcct tgtgcttctc
     9781 ccgaacttta agcatttccg cttcaatctc ttgatctgga tttttacggt ccaatcgttt
     9841 ttgccagtac ataaagattg ccttagggaa ctttaaagtt tctagaagct caactaattt
     9901 aaacggtcct cggaggcttg cgatgattcg tgcgattgct tcattcgtcg ttgttgggct
     9961 tcctgttttc gcagcttcct caattctttt aaaaaggcat tttcaatttg aagggaacgg
    10021 actcgtttct ccaactcctc gatgcgttca cgttctttag acgttgcttt agttgtagat
    10081 tttttcttat ttttgggttc atttttctta gacaagatag gtggacatcc tttcgttttt
    10141 gagagtccat cgatcccacc ctcacggaat tgacgcatcc aactagcgat caaattaggg
    10201 ttgttcatgt taagtgtgtt ggcaacttca cgataggaca gctcacttgt tagatataac
    10261 tctatcgcat ccagcttaaa ttgaacagaa tattttttct tttgtcgctt tcttactaga
    10321 ccctcttcac caaattcttg ataggcattg atccagcgct tcacttgtga tggagcctta
    10381 atggaatatt tctttgccag ataatcactt ccgcctttac catccaagta atcatgaacg
    10441 atctttaatt taagttcaaa agtatactta gccataaaaa ataccgacct ccaaaagtta
    10501 gattttttgg tctaactttt gggggtcggt acaaaactta aaagttttag acccttttat
    10561 tttcttaatg acttcagtca gttttgtgcg taaagcgtgc gaaacaagaa aaccgcgcgt
    10621 ttctacgcgt ggaagaagta tctttagcga taaaaactcc tttcgatata ctagtgagtg
    10681 gctaccaacc gctcatcaga tcgaaaggag ttttttcatg tcaaatgacg ataaaagttt
    10741 agcatatact cgttggaatt gtaagtatca tattattttt acaccaaaat atcaacgaaa
    10801 agtaatctat gggcaattac gaaaagatct tggaaaaata cttaggaagt tgtgcgaaat
    10861 gaaagatgtg gaaatcatag aagcacatgc aatgctagat catattcaca tgcttgttcg
    10921 agtaccacca aaaatatgta tatccagttt tatggggtat ctgaaaggtc gtagtgcggt
    10981 actaatccac gagcagcacg caaatctcaa atataaatac ggaaacaaat ccttttggtc
    11041 gaagagatac tatgtgagta cagtcggatt gaatcaaaag acaaatgaaa aatacatccg
    11101 tgaacaagaa gctgatgatc gcattcgata tattataagt aaaagagagt acgtagaacc
    11161 atttaaaaaa taaagagaag taagtgagtg aagatgagca gttggcggtc ttttaaaaat
    11221 ccccttttaa ggggcagcga gtgtcgaccc cttctagggg gattaaaaat acacccgtcc
    11281 tcacgggtgt atgggttact gatagacttg attttaactg gtgtcagaaa cttatgtcca
    11341 gaaagggtga tgaaattttg gcaatttacg gctattcaag aattagcaca agcaaccaag
    11401 attacaaaac acaaattcaa acattagaaa atgctggcgc agaaataatc ttcctagaaa
    11461 atcactggat tatcgaacac ctttggaagt atttttgagt tacgtaagta ttgatgatct
    11521 gtctaactta atttgacaat taagagtata tttacttcag atggtaaggc aacaaaataa
    11581 aatacaatag gcagatgaac taataaataa aatctgtatt aaatacagat aaattaatat
    11641 aaaaatcaaa aggatcataa acttttaaca agtgatgaaa tgggtggaaa ttgattctta
    11701 tagacagttt gtctcataac attgaagtat tttgcaattt tagcattgaa acactttttt
    11761 aatcttcaac gttgttctta taaacaagat gattgggaat cttttgaatt aacactgtat
    11821 agcttagaac accctttata gaaaacattc gcttagcaat cttaataatt ccttgtgccc
    11881 gatgtcgttt agattcagta ggttcctgct catcgatcat ggaaaaaatt tgaaggatca
    11941 gatccttaat aaatttattt aagagaggat gatccattgc ttcatccata ctcgataaac
    12001 tagtgataat gagctacacg tttttctttc aagttgatca atagcttcca cgataaaaat
    12061 atctttagat aaaatcaaga accgtaggaa ctacagggat atttggtcaa taagagccac
    12121 cgctattagt aaaaaataag ctatcaagta tgctctcatc ccaagaattg cctacttcaa
    12181 tcgttaaaaa ccgttaggtt tagctaagtg ggggtagttc actcaatact tttatcaata
    12241 atagagcgca aaactttttc tcttaattca actgaatcaa tattattgtt ttgttcataa
    12301 ccatgaacta atagatcaca aatataaagt tgtgatattt ttccagctaa agatccacca
    12361 ttaagaaatt cttcaatggc tgtttgtaaa actaagtctg cagactttcc tataggtgaa
    12421 tgaatatagt tagttatagc aataattttt gcgccatttt ttttagctat tttcaatgaa
    12481 tcataagtat ctttcgtttt cccagataaa gaaaaaatga ttactaaatc attaactgtt
    12541 aataaagaag cgacttgggc ttgaaaatga gggtctagta cagcttttgc ttgaactcct
    12601 acgcgaagaa acatattttc taaatcaaga ctagtatttc cactagatcc cactccaaat
    12661 atatatatgt tttttgattg agtgatcatt ttaatggctt cattcaattt ttcttcgtct
    12721 aaaagcctaa tagtggaata gagagcttct gttaaactat tagcaatttc atcatagtac
    12781 tttacactag tcggatgttc ttttttttga ctaaagtctt cctttgcaat ttcaattttt
    12841 aaatcagaaa aaccagaaaa accaattttt tgacaaaagc gaataatagt agcatcgcca
    12901 acttttgtgt ttttcttaat atcactcatc gtactataaa taatagtttt cccagaattt
    12961 aaaataaaat cagcgacttt tttttcagac ttcgttaaaa atggataatt ttctttaatt
    13021 aaatagatat aactcataca aactcctcta caatttcaac ttttctccat tatactccaa
    13081 aataataaaa aaaactccta aatactccat ttttatcttt acttttatga aagcggatgc
    13141 tatattgttt ttggagttat tatccaaaaa taataaaaaa ttggagcgtt taggataatg
    13201 gataaaaaga ggtttttaga tcttatttca aaaggtttga ttgtttcatg tcaagcatta
    13261 cctggtgaac cattttacaa agaggaagaa agtttaatgc cttattttgc tttggctgct
    13321 caaagggctg gagcagttgg tatacgtgca aattctgttc gagatattat tgaaattaga
    13381 aagtgtgtat cattaccaat gattggaatt attaaaagag attatccacc agaatctcca
    13441 ttcattactg ccactatgag agaagtagat gaattagtag aaaccggtgt tgaggtaatc
    13501 gctcttgatt gcactcttag aaatcgacat gatggactat ctataaattc ttttattact
    13561 aaaataaaag aaaaatatcc tcatcagtta tttatggcag atatttctac atttgaagaa
    13621 ggcaaaaatg catataaagc aggaatagat tttgtaggaa ctactttaag tggatatact
    13681 gaggaaagta aaaaacaaga tgggccagat atagatttga ttaaaaaatt ggtagaagaa
    13741 gggatttgtg taattgctga gggaaggatt catactccag aacaagcaaa agagataaaa
    13801 aaaataggag ttgcaggtat tgttgtgggt ggtgctatta ctcgaccaca ggaaattgca
    13861 tcaagatttg ttaaagcatt aagttagggg gagaaataaa atgtttaaaa aattttcaca
    13921 attaggacgc gctttcatgt taccaattgc tatattacca gtagccggat tactattggg
    13981 actgggagga gcgttaacaa atgagagtgc tataaatgct tatccatttt tagatcaacc
    14041 gtggttgcat acaattttaa gtattatgag ttatgctgga aatgcagttt ttgcaaacct
    14101 agcactaatt tttgcaattg gtattgctgt aggacttgca aatggagata aaggaacagc
    14161 tggtttagct ggtggagtag cttatctagt atatactgca actattagtg ggtttcttga
    14221 acttttttca gcaaaagatg caacactaga tacaggtgta gtgggatcaa ttgttgttgg
    14281 tggtactgtt gcatttttac acaatcgtta tagaaaaatt gaattaccac aattcttagg
    14341 tttttttgga ggatcaagat ttattccgat tgtttctagt ttagctgcaa tcattattgg
    14401 ttctttcttt tatttaattt ggccaccaat tcaaaatggt ttagtagttg ctggtgaaca
    14461 aattgcccaa atgggtagtt taggaacttt tttatatggt tttttattgc gtttaacagg
    14521 tgctgttgga ctacaccaca ccatttaccc tttgttctgg tatacatctt taggtggtac
    14581 cgaaactgta gctggatcaa caattgctgg agcccaaaat attttctttg cccaactagc
    14641 tgatccaaac cacactggtc tgtttactta tggtactaga ttttttgcag gaagatttgc
    14701 aacaatgatg tttggattac cagcagcatg ttatgcaatg tatcgtgcaa ttcctaagaa
    14761 aaatcgtaaa aagaatggtg ggctttattt tagcggggcc cttacatcat ttttaactgg
    14821 aattactgaa ccagttgaat atatgttttt atttgttgct ccttggcttt atgtgattca
    14881 tgcattctta gatggattat ctttttattt tgctgatatc ttaaatattc gtgtagggaa
    14941 ttccttttca ggtggtttga tcgacttttt attattcggt gtacttcaag ggaatgacaa
    15001 aactaattgg ttaaaattaa ttccttttgg tattgcatgg gcatttatat atttctttgt
    15061 ttttacattc tttatcaaaa aatttaaggt agctattcct ggaatggaag aagaatcaga
    15121 agttcattct gaaaaagtaa taaacaaaaa tacatcatta catgaagagt ctcttatcat
    15181 tattgaagct cttggtggaa aagaaaatat tgagtctgtt gaagcttgtg caactcgttt
    15241 gcgtgttgct gttacgaatg gggagattat tcaaaaacaa acaataaaag acactggtgc
    15301 aactgctgtc ttcgaagtga agggtggcat ccaagctgtt tttggaggga aagctgactt
    15361 attaagtcaa gaaatcaatc aaatattagg aaaagataat taggaggttt ttaatatgtt
    15421 tgaaattttc aaaaagaaaa aacaagaaac tttttatgca ccttgttccg gaaaaatcat
    15481 ttccatcaca gaagtaaatg atcctgtctt ttcacaaaaa ttactaggtg atggttttgc
    15541 cgtcatacct gatggttcaa aaatttatgc tccattggct ggtcgagtaa caaatatatt
    15601 tcctactaaa catgctatta atataaaaag caactcaggc attgattatt tgattcatat
    15661 tggaattgat acagttgaat tacaaggaga gcctttttcg atttgtgttc aagaaaatga
    15721 tgaggttact cctgaaacct cgttggttga tgtggatttt gacaaaataa taaaatctgg
    15781 taaagatact tctgttatag ttgtttttcc ggagaaaaat cagttcaatg atttaataat
    15841 agataatggt cgaattgctc atgggaattc atgtggaaaa atcacttttt aaaaaagtgt
    15901 atataaaaag ctataaaata agctaaatag gatatttgtc aaatgaagtt ggtgaacaga
    15961 aagaactgtt ttctttctgt tcattttttt attttattag taatgattcc ccatgccaaa
    16021 tcatgatccg taatttaaag ttataaaagc ttcgaaatcc attggcattt cgttggaata
    16081 ccttgatatg attattcaaa tataaaatat agagagaaga gagagaggga agatatttat
    16141 aaagggcaaa gttatgttta ctcaacttag gaaatagaaa tagatcaatt tatattctct
    16201 tgtcaatcga atatgaacct tagcactata taccaaatat agtaaaatgc tgaaagatgt
//
LOCUS       DASJON010000047        15847 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJON010000047 DASJON010000000
VERSION     DASJON010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.047"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1545
                     /locus_tag="U3989_001131"
     CDS             <1..1545
                     /locus_tag="U3989_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1714590.1"
                     /translation="GNPLFEDLSCQIEAGEKIALVGINGTGKSTFLQILTGREGVDSG
                     VISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRYEALMTEPDTNLEK
                     CLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELSGGERVKVALAKIL
                     AADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFLDQVTTKTWELEEG
                     ALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFRQWAHEGDNESFFK
                     KAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLHQQYADQVLFKDSS
                     FAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQIGYLPQQLQFADPD
                     SRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIRLALLKLFLEKINV
                     LILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQEALVIDQEQISRQP
                     LGQLID"
     gene            complement(1982..3166)
                     /locus_tag="U3989_001132"
     CDS             complement(1982..3166)
                     /locus_tag="U3989_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN1714591.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(3191..4198)
                     /locus_tag="U3989_001133"
     CDS             complement(3191..4198)
                     /locus_tag="U3989_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1714592.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(4370..4729)
                     /locus_tag="U3989_001134"
     CDS             complement(4370..4729)
                     /locus_tag="U3989_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714593.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(4701..5072)
                     /locus_tag="U3989_001135"
     CDS             complement(4701..5072)
                     /locus_tag="U3989_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN1714594.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(5089..5391)
                     /locus_tag="U3989_001136"
     CDS             complement(5089..5391)
                     /locus_tag="U3989_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714595.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(5408..5878)
                     /locus_tag="U3989_001137"
     CDS             complement(5408..5878)
                     /locus_tag="U3989_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN1714596.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(5853..6131)
                     /locus_tag="U3989_001138"
     CDS             complement(5853..6131)
                     /locus_tag="U3989_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN1714597.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(6128..7177)
                     /locus_tag="U3989_001139"
     CDS             complement(6128..7177)
                     /locus_tag="U3989_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN1714598.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(7134..8063)
                     /gene="tadA"
                     /locus_tag="U3989_001140"
     CDS             complement(7134..8063)
                     /gene="tadA"
                     /locus_tag="U3989_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN1714599.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8195..9949
                     /gene="ade"
                     /locus_tag="U3989_001141"
     CDS             8195..9949
                     /gene="ade"
                     /locus_tag="U3989_001141"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN1714600.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(10047..10709)
                     /gene="trmB"
                     /locus_tag="U3989_001142"
     CDS             complement(10047..10709)
                     /gene="trmB"
                     /locus_tag="U3989_001142"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN1714601.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10779..11561)
                     /locus_tag="U3989_001143"
     CDS             complement(10779..11561)
                     /locus_tag="U3989_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN1714602.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(11765..12976)
                     /locus_tag="U3989_001144"
     CDS             complement(11765..12976)
                     /locus_tag="U3989_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714603.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(12969..13706)
                     /locus_tag="U3989_001145"
     CDS             complement(12969..13706)
                     /locus_tag="U3989_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714604.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            13844..14278
                     /locus_tag="U3989_001146"
     CDS             13844..14278
                     /locus_tag="U3989_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN1714605.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            14271..14615
                     /locus_tag="U3989_001147"
     CDS             14271..14615
                     /locus_tag="U3989_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1714606.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            14795..15805
                     /locus_tag="U3989_001148"
     CDS             14795..15805
                     /locus_tag="U3989_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1714607.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         5026 a         3264 c         2766 g         4791 t
ORIGIN      
        1 gggaatccat tatttgagga tttatcctgt caaattgaag ctggtgaaaa aattgcctta
       61 gtcggtatca acggaactgg caaatcaacc tttttacaaa tcttgaccgg cagagaaggt
      121 gtcgacagcg gtgttatcag tcgcaaaaaa ggcttaaaga tcgggacggt cgaacaagaa
      181 cttacagttt ccgaagcgac cgtcaaccac tacttgctcc attctgccgc tgagatacag
      241 gacctcaaac agcaaatgag tcgctacgaa gcattgatga cagaaccaga tacaaactta
      301 gagaagtgtc tggcccgtta cggtgagctg cagcatcgct ttgaagaact aggcggttac
      361 gtattagaag accggattgc agcgatcttg caaggcttag ggattccaca ccgtcaggaa
      421 gcacggttaa cagaacttag cggaggcgaa cgagttaaag tcgctttagc caaaatttta
      481 gcagccgatg ccgatttact tttactcgat gaaccgacca atcatttaga tcttaacagt
      541 attcgctggt tggaaaacta tttacagaat acaaaaaaat cggttctggt cgtttctcat
      601 gatcggcaat ttctcgatca agtcaccaca aaaacatggg aactggaaga aggtgcattg
      661 attgagtatc ccggaaacta cagccgtttt cgagtcttga aagaagctcg cttggctgag
      721 ctgacaaaaa attacgaact gcagcaaaaa gaagtccagc ggttgaaagt catgattcgt
      781 cgttttcgtc agtgggcaca tgaaggagac aatgagtcct tcttcaaaaa agccaaagaa
      841 ctggaacgcc gcctggcgaa actgacactc gtcaaaccgc cacctccccc gaaaaatcgg
      901 ctgcaaagct tgtctaatgg tggaaaatct ggcaaagaag ttttcatcat tcaaaacttg
      961 caccagcagt acgctgatca agtactattc aaagacagca gttttgcagt ctatcgtgga
     1021 gatcatttag ccatcatcgg cgacaatggt gcaggaaaat caaccttact caaattatta
     1081 ctcggtgagg aaaagcctta cggtggcacc atcaaattag gtagtagctt gcaaatcggt
     1141 tatttgccgc agcagctgca gtttgccgat cccgatagcc gtttgctggc ttacgccatc
     1201 acattgactg gaaacgaaga agtcgctcga cggcaattag ccaaaagtgg cttttatcaa
     1261 agcgatgtgg gtaaacggat caaagattta tccggtggtg aaaaaatccg tttggcgttg
     1321 ctgaaactct ttttagaaaa aatcaacgtc ctgattttgg atgaaccaac caatcacctc
     1381 gacagctatg ctcgagaaga aattgaagaa atgctgcaag actatcaagg aacgctccta
     1441 gcagtcagcc atgaccgcta ctttttgcag caacacttcc aagaagcact cgttattgat
     1501 caggagcaga tcagccgcca gccgctcggc caactgattg actaggacgt tttttcgcaa
     1561 caaaaaactt gattttcata cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat
     1621 caagttttta ttattcgatc aatgaatgtt tcggatcggt tttctagcta aacataaagc
     1681 gaagaataaa gtcgacagcc cacgaggctg atctaaaaat gttttttctt tttacatatg
     1741 taatgtccct tataaagatg aatgaaaata aaatattggg attttcctga aaatcaataa
     1801 ataaaatgcc agtcttttcc cttttgacca cttttccaag caaaattccc taagaagatc
     1861 ctgtaaaaaa gaagggaaat tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa
     1921 aacacatgca agaattccct cttgcatgcg tttcatcttt tatcctaacg aatgctttgg
     1981 cttagccgcg cagacgctcc acatcacggg caatcattag ttcctcatct gttgggatca
     2041 ctaatactct tacttttgat tcttttgtag agatatctac ttcttcacca cgcaattcat
     2101 tcaacgctgg atcgatttca caaccaaacc aagacatacc tttgatgatt tcactacgta
     2161 catgtccgtc attctcgccg ataccagcag taaagacaat tgcatcaaca ccattcatcg
     2221 ttgtcacata accgccgatg tattttcgga tgcggtccac gaaaatgtca taagctaaac
     2281 gaatatgttc tttttcatag ttcttttcaa gatcacgcat atcactagac aatcctgtta
     2341 ggcctagcag accagatttc ttgttcaaaa cattgatcat ttcatcaata tcaatctcta
     2401 atttttccat taaatatggg agaacagctg gatcgatatc acctgaacgt gtacccattg
     2461 tcaccccagc taatggtgtg aatcccatag aagtatccat ggatttacct ccatcgactg
     2521 ctgtgatcga tacaccattt cctaagtggc aagtgatgat ttttagttct tcgatcggtt
     2581 tgttcaataa ttttgccgca cgttccgaca cataacgatg acttgtgcca tggaagccat
     2641 attttcttac tttgaaatct tcataataag acaccggtaa gctatataaa taattttgct
     2701 ttggcattgt tgagtggaaa gatgtatcga atacagccac actcaaaatg tcaggtaaaa
     2761 tctttttaaa cgctttgatc cccattaagt ttgcaggatt atgcaaagga gcaaattctg
     2821 ctaattcagc aatgcggtcc attactttct catcgatcac tactgaatcc ccataagttt
     2881 ctcctccgtg aacaacacgg tgccctactc cggtgatttc atcataagag cttaaaattt
     2941 gcagttcaat caactgatct aacagcattt ttactgctac ttcgtggttt tcgatatcca
     3001 tgatttgttc gaatttttga ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac
     3061 cgatacgttc gacgatccct ttcgcaacta cggtttcttc tggcatttga tataattgcc
     3121 atttcaaact tgaactccca gcattgattg caattgtttt agacatatga ctctccttct
     3181 cgttgaatga ttacagagca gatgatttcc agtttttaaa ctcgttgaaa aattccgtca
     3241 ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa gacttctttt acttgttgtg
     3301 cttgtggtcc ttttttctgt aagatcaaaa tacttttttg cgattgttta ttacggaaca
     3361 attcatctgg caattgaatc attccttgaa gataaccttc ttctttaaac cattttttga
     3421 tttcttcact ttgatccgtt tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat
     3481 ccggcttcac atatttcatt gattgctcta gcaataaatg atgtgcataa ctatggcctt
     3541 cttcggtact tgttaaaaac tcttttgctt tttcgtcatt cggataataa ccgattggca
     3601 aatcgctgat tgcaaaatcg acaggatcta ttaataattc ctgcaacccg tcttgatgaa
     3661 agtattgaac atttgcttgt gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat
     3721 catcaatatc cacaccaatt ccttctgttt ggtaccccgc attcgatagg tttaaaagaa
     3781 ctgttaataa caagttgccc attcctgccg caatatccag aatcttaacc ggtgcttttt
     3841 tatttgtgta tagttgttca ataagaaaaa caaataaaaa accgatacta tcaggagtga
     3901 gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt caataaaagt aattgagaca
     3961 gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga
     4021 tccgttgcgt tgtctctttt gtcggcactc catccaccac acgtacttga taatcatcta
     4081 ttaaattttc tgcattttcg acataggctt ctaaaaagga agttcccaat gcattctgta
     4141 aaagctgaat agcttccagg ttttgattaa acccttgttc cattttttct ggtaacatgg
     4201 tgcacctcat ttctagatat attataacaa ttttttcacc tgtttaattc tatcgaacaa
     4261 ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg aaacggcaaa cattttttta
     4321 ttaaaaacag tgttataaca gaaaagaggg cgttttcacg aacattccat cattctgtac
     4381 tagtatctgt atcatttttt acttcttctg tgaaccgaaa gataaacggt gagacttcca
     4441 cctctaagaa tagggactgt tgtacccact gatatgttac agatccagtt gtgtagtaaa
     4501 aaactccttc cgaactttcg gcaagcgtag actgttccga taaagccatc tgcttcatta
     4561 tttttgctat ataataattt ttgattcgta tattaagatc cattgacaaa cggtagcttt
     4621 ctaatgctgc catcaataaa aacgtacata aaagaaataa aagcaagaca gaaaataaaa
     4681 tagagccctt ataacatttt ctactgatcg ttttttgtcc atatggcata tccatcttct
     4741 ccattttcaa agtgtacata aaaagaaatt gtgtgttctt tttccaataa ttgaagctca
     4801 gtgatacttg ttaacatagg ttgatgtccg ccggaagttc ttcttttacg tatcattgcc
     4861 tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc
     4921 acagagacat tgtgactgct cttgagttct tcttctaatt gtgctaaaaa tattagccac
     4981 tctttttgat ctttacgctg caaaaattca cttacttgga tggtttgctg aatcaaaaga
     5041 ttgaatgtga gcaatagact gctcaatatc atcagtccaa ttagacattc aatcagcgta
     5101 aatgctggaa ttacttgttt tttcaatact gatcgcttct ttctccatat tgacaaacac
     5161 tttttcgatt actccattat tatcgacaat ttggatatcg tatgggatca tttcttccct
     5221 tcgctctggt cttttgtgat acatccgctg cgtcttgata tcttcataaa tcattcgaat
     5281 aaatagcagt tcctccccac ttttcttttc tgcctgaagc aaaaacagct ggccaaagct
     5341 cactaagtag acacagacag aagcgatcat aagcgctgta agggactcca acaaaacaaa
     5401 tcctgtttta ttcaattctt tttgtatagc gaccactccc tatctggaat tgatacgtaa
     5461 tcgtccgctc tttttcttcc caataaaatt gataagcttt tagtgaactt tcattcccgg
     5521 ttctagcagc aaaagtaata gaagcatggc gtttcaaggt aatctcatca ggaatatcta
     5581 atacttccca atttatttta tctggattcg gaacatgaaa aatgatttga ttctcttcac
     5641 tattccaata aaagcctgtt tggatctgat tcacaatagc gattttttgc gtagaataga
     5701 ttcgtttttc aaattggccg aaaaattgat agactgccaa ttcattcttc catgcagaaa
     5761 atgcgagaac tggaaaagaa aggatcgttg taagtaataa aatcagtaag agtgattcaa
     5821 acaacgtata tcctgatgct aaaaccttac cttcaaggcc gctgatagat ttctgcatgt
     5881 tctcgtttga tatagccttc attcaacaat tcctccacag taggaactct tccattattt
     5941 tgaagcgcat agagctcttt ttgtgtgtct accaactgga tgatcgcttc acgactttct
     6001 tggtctacat gattcctata acgcgacaaa ttagggacga acaacaacac taatacactg
     6061 atgatcagta agacgattag catttcgatc aatgtaaaac cgctataata agatattttt
     6121 ctctttttca tggcaaaacc tcctctatgc ttccgtaaac aggtaataaa acagcggcat
     6181 aaactaataa aatcaaagcc gctacgccca aaaaaatgat tggctgaata aaatgcatcc
     6241 agtgatgaag acgtttgaaa agctgttttc ttgtccattc gctgtaaaaa atcagttctt
     6301 tacctaaact tccctttgcc tctccctgta aaacaatttt actgaattct tctgatagaa
     6361 aacgataatt ctggaactgc tcagccaatg acatacctgc ctccaatcct tgaatcaact
     6421 tagaggcaag atgctgaatc agactttctt gtttcgtttc ctttaagcag actatgattt
     6481 gccttaattc gaaaccttca tgaaaaagct ttccaatctc tagtgacaaa taagccgatt
     6541 gatacaagga atatatcggt ccaatcaata attgtctgct tatccattca ctctgatcaa
     6601 caggagacaa cttcatcaga cgatacctta ctagtaaagc caatagagta accgctagga
     6661 tgaatagtag aacataccaa tgaaagactt gtatcagctg aataccccaa tgctcttgca
     6721 tgaccattcc tgaaccaata agctgaggca aaacgaacaa ccgcatacta aacaagatac
     6781 ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag ctttttcaaa tcattgcgaa
     6841 atccttcaac aagccgaaac tgtttggcaa tttccaacaa agttgtcggc agattcccat
     6901 gacgttcagc taactcgatc tgtaccagtt gctctgctcc atatcctatt tgcgaaagta
     6961 cctcactaaa cgcttgccct tcctttagct gatgttggat atttgccaaa ataacaggtg
     7021 gaaacgaacg gatcgtgagt aataaataaa gggattcttg cagactaaaa ccagataaaa
     7081 gcagttctcc catggtttgt gcaaactggt tttgctggct tcttgttaat cctttagaat
     7141 agttctgact gccaaatatt ttcctgagct tcttcataac tttcctccca tgattttttt
     7201 actgattctt cctccataaa ttgataactc cataaaacag aaggatggtg attggcatct
     7261 tccaaaagtt cttgataaat gacaccagct aaacattcag atagctcttc ttttttcttt
     7321 gtcagttcat ggattctgac caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa
     7381 acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg cagttgattc atctcttatc
     7441 tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa
     7501 gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa acggttcttc tatctctaca
     7561 ggatcttcga tagtaatcac ttgcaatttt tcttccaaag ccaaccgata cattagagaa
     7621 gtcttgcctg atccgactgg tccgctgaaa aggtataatc cacgtctgac ggttttctgt
     7681 aaaatagttg caaaatctcg agaactaaag caacgatggg gtgtttcaaa agtaccgtat
     7741 aatagccgaa tcactaaact ctctcgttga aggtaatctc ctacagaaga caaccgcaga
     7801 cgaacttcct gtttttctaa agaataagta aatgcaccta actgagcttt tcttttttct
     7861 ccgatatcca tctgtcccaa atacttaaat cttgagatca gttgctgtcc atctgccgaa
     7921 gagactttct tccagaatgt tcgttttttt ccatagcgaa aatagatgta aaatacttct
     7981 gaagctggca atatatatac gtcttgggca tgtttggcaa aactttcact tatcatttga
     8041 tccgataatc gttttatttc cattattctc ccctccacaa aaaacatacg tctttttgat
     8101 ctttttgttt caaataaaat gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt
     8161 atacttataa aaatttatct tgggaggcac tccattggaa acaacattaa ggaaatatat
     8221 cgaagtagca agtaaaaaaa agcctgctga cctagtgatc aagcatgcta agatcgtcaa
     8281 cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg
     8341 aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat gggcaatatc tagtgccagg
     8401 attcatcgat ggccatgtgc atatcgaaag ctcactttta tcaccaagcg aattttcaaa
     8461 agtatccttg cttcatggag ttactactgt agtgacagat ccccatgaaa taggaaacat
     8521 tgctgggagt acaggtcttg agtttatgat agaagatgca agacaaacac tcatgaatat
     8581 cttttttatg ctaccttctt gtgtacctgt aacccctttt gagaccaatg gtgcaaattt
     8641 aaatgcaaaa aaattagccc cttttttaca aaaacccgaa gttttaggct tagctgaagt
     8701 catgaactat caagcagtag caaataatga aacagacatc ctagaaaaga tccaattgat
     8761 gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt atcggcatgg aagagttaaa
     8821 tgtttatcct gcagcaggta ttcggacgga tcatgaagct acaactgcaa aagaagctaa
     8881 agaacgtttg gatcttggta tgtatttaat ggttcgagaa gggaccgttg caaaagatct
     8941 ggcatcgcta ctaccggcga tcacacctga aaattcaaga agatgcttct ttgtcaccga
     9001 tgataaactg atcaatgatt tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc
     9061 tattgcactt ggaatcgatc cattacaagc cattcagatg gcaacattga atgcagcaga
     9121 atgttttgat ttgaaaacac taggtgcgat tgcccctggg tatcaagctg atttcttctt
     9181 aacagacgat cttaccaagc tgccaattca tgaagttttc tataagggaa cattagttgt
     9241 tcaatctggg caactccaag accatttatt tgctgatcat tcaaattcat atacgtgttg
     9301 tctgccaaaa ctaaacgctc aaccattaaa tccacattcg ttagagctgc ttctttcttc
     9361 ttcacaagcc catgtgatcg aaattatccc gaatagtcta gtgactaaag atttggtaga
     9421 aaaagtggac cgacaaggcg aacgattcat tccttcaatc gaaaaagacc aattaaagat
     9481 tgcggtgatc gagcgtcacc atcaaacggg aaacattgga ttaggcatag tgaaaggatt
     9541 tcagctgaaa caaggagcga tagcgacaac gattgcccat gactcgcata atctagttgt
     9601 cgttgggaca aacgacgagg atatgctgta tgctgctaat cttttgattc aaaaaggtgg
     9661 cgggatgacc gttgtcaaca aacagcagga aatagcttgt gtttctcttc ctataggcgg
     9721 aatcatgaca caggaacctt tttcgatcgt ctatgaacag ttgaatgaat taataactaa
     9781 agcacatcaa ctaggagcag ctaaaacctt tgatcctttt ttgacacttt ctttcttaac
     9841 attatctgtc atacccgaat tgaagatcac cgacaaagga ttattttcat tttctcgctt
     9901 ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat acattttaaa tcaaataaat
     9961 cccagaaaat acattacatc tgtaatgttc attttctggg atttatttat ggatattaat
    10021 gtttttatga aagcagaaag ttagtttcac agacttttct ttttggtctt atcttgaaat
    10081 tgagcttcta ctcgataaat acgttgacct tttgaagaaa atttttcttc atactccgtc
    10141 ataatattgc cttcaaattg actttgatgt aaatctaacc atacctgttt gaggatcatg
    10201 ccatattgag aaaaacttgc cagagaatac tcaaacaatc cttgattatc tgttttgaaa
    10261 tggatttctc catttggacg aagaatctgt tcatctacag caagaaaagt cttatatgtc
    10321 aatcgccgtt tttcatgacg tttttttggc catggatcgg aaaaattcag atagatttga
    10381 tctacttcgc tgtcagcgaa atactctgtc aacgctgatc catccacatg tagaagtttc
    10441 aggttaggaa gcggttgttc gatcaacttg tctagtgcaa tcgaaacaac actgacctgc
    10501 atttctactc caatataatt gatttcagga tgggcttttg ccattccagt gatgaactgg
    10561 cctttcccca taccaatctc aatgtggatt ggatgatcgt tttcaaacaa ttcattccat
    10621 tttcccttcc acaatgttgg atcactaatt acaaaattcg ggtgagccgc taacatttcc
    10681 gctgctcccg gacgatttcg gacacgcatt atttatctcc attcctttcc tctaattaaa
    10741 aaggtctggc caaatctccg cagagtttgg gccaaacctc agccactgaa aattcgtttt
    10801 aattgtaaaa tttcttcgtt catccgtcga tgatcttcac gtttgcaata tctggtgatt
    10861 tcttggagca aattcatggc cgcataccaa tgaaattttt caagcatttc gtcagtcggg
    10921 cgtatgccat aactgatcag ccactgattc cagctagaca gtggcacata atttcctaaa
    10981 atcgttgcaa tatccaccgc agggtctgca aacatgattg aatcccaatc gactagatat
    11041 aaataatttt tacatactaa ccaattacga tggtttacat ctccgtgtac tactgataag
    11101 ttttccttat ctattgacgg gatctgctca cgaagaaaac gatggacaat ttgcagaaat
    11161 tgatttttct ggagttcttc cggtaattct ttttcatatt tttgcagcat cttctccggc
    11221 gtcatgacac gaccaccgat acgggtcagc atggttttaa gagaatttga atgatgtaaa
    11281 tggtacaaaa catcaatcac gtcgtttctt tgaccaatct cattaggagt cagcaagcga
    11341 ccttccagcc actcttgtgc tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca
    11401 agttttggtg taattccttc ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta
    11461 ataaagactt tgtcctcatc acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg
    11521 ggacgtaacc gccactcttg gtctaactga aactccatca tgcattctcc cgtcctctta
    11581 ctctttgttt tgcagtactt attattctag tcgcctgaac actacacgtc aagcacatat
    11641 acgttctttt tcatttaatt gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg
    11701 aacagcattc ttactgtcaa cgctctattt ttcattctca tttttaccct acacagcttt
    11761 gttttcagtc tgacatcttc ttcaaacgag ttggtacata tagtttaaca aagagcatag
    11821 taacaatcaa ataagccgct agcggaaccc atcgctgctg cccatctaat acgactgcag
    11881 caatcaaacc aaaaataacc gacgcgataa ttaataccca tgtgagtaga tactgaatcg
    11941 cctgcttctt ctgattctta tggattggat aaagctgaac gagaaccata tagcggaact
    12001 ggttcgctaa aggtaacagt tggaaaccga tcaaatataa aaataagaaa ccgataatca
    12061 gactaaaata aaagtcgact actccggcaa tcaataaccc acctataatt actaatcgga
    12121 aataaagacc actaaaatca gaacctcttg ccaatcttct tgcaaacaag taaagataag
    12181 tgttttttga ttctctttta atatgagtca atataggatc caaatagcgt cttcttttga
    12241 tctttgccgt aatttcagga acatctgtga ataaattgat aaattgatag atctgatgca
    12301 acctatgttc ttctacatgc accattttct cccaatccaa tcctttttct tgttgcttcc
    12361 acaaaaacca gtaatataga cttgcctgta ctaatgcacc tgctattcct gcccatatta
    12421 gccaataaaa tgatagaaac aaaaagatca gagaagaaaa aaaccagcct aatttccaat
    12481 aacgaataac ttgttgatct agtcgaaaca attctgctct cttgatttgc agatgactga
    12541 atttcaaact ccataatatg atcagataga cgaaaaacat cgaaaaggat tgctccgtac
    12601 taaccaccac taatggcatg gccaatccgc atgccaatat caaaaagatc aacggaaaaa
    12661 tgcaagaata gcgaaaagct cgttccaaat aggtttgcat ttctttttct ttaggaagta
    12721 aaaaaacaat atctgctgcc tctgcaaaag tagccatctt acccacgaat aaacagagca
    12781 tccaaaatac agagattatg agccctccaa ttgcaaacgg tggctgcagg gttttcaacc
    12841 aattagaata atataatccc acaccgccaa tcaagaaaag acaaactaaa acaaaatgat
    12901 cgttcaatac ataacgcata tatttcatca ttttcttttg atgtcttgct aaacgttttt
    12961 gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa tatagatttc gtccaaagaa
    13021 gaatccggca gctgaaattc ttttctcaac atctcaagag aaccgtctgc tcgtatctct
    13081 ccgttatgta agaccacaaa acgatcacaa tatttttctg cagtagctaa aatatgcgta
    13141 gacatcaaaa taccggctcc ttcttttttc atttctacca tcaattcaag caatgcattg
    13201 atagctagtg gatccaagcc taaaaatggt tcgtcgatga tatataaaga aggctcgatc
    13261 aaaaaggcgc ataagatcat cactttctgt ttcattccct ttgaaaaatt agctgggaac
    13321 cagtcaagac gattagataa ccgaaaagtc tccaacaact tttctgctct tttcattgct
    13381 atatccatag gtatatcgta ggccatcgca gtgacttcga tatgttctct caatgtcaat
    13441 tcttcatata aagaaggagt ctctggaata tacccaattt gtttgcgata ttcttccgta
    13501 tttttcttta aggatagatg attgatttct atcgttcctt ttgaagcttc cagcagaccg
    13561 ataatgtgct tgatggtcgt acttttccct gctccgttca gtccaatcaa ccctactatc
    13621 tctccaggct gtacatcgaa actgatatct tttaacacag ggagatgtcc gtacccccct
    13681 gtgacatgat cgataattaa actcattctt tttcctccgc catttatttt tatttattat
    13741 accacgaaga aaatgacggt attttgtttt tttccttaca atagctttaa cttatggtaa
    13801 agttaagctg aagtatttat tctttataga aaggatgttt tatatggaaa actgtatttt
    13861 ttgcaaaatc atcaatcaag aaatcccaag ctataaaata tatgaagacg acaaggtcta
    13921 tgctttttta gacatctctc aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca
    13981 tgtagcagat atttttgaat atgatcccga acttgcagga gaggtttttt cgcgtgtgcc
    14041 taagattgct caagcattgg aaaaagcttt tccagaaatg aaaggtctta atatcttgaa
    14101 taacaaccgt gaactagcct atcaatctgt ttttcattca catatccatt tagtccctcg
    14161 ctacagcaaa gaggatgatt tttctattca ctttgtcaat catcaagata gttatggatc
    14221 ggaagaacta aaagctattc aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa
    14281 actttttcaa aggactgtta tttggagctg ctgctggcac tgtcggaggt ctgttattgg
    14341 cgcccaggag cgggaatgaa acaagaaaga aactgatcga agaattggaa gaagcaacaa
    14401 ctctgacaaa tgatttaaat aacagcctga atcattttaa aagcgcattg atcgaaacaa
    14461 aagaaacagc tgaatctgtg attcctgctt tccaagaagc aatcaaaaaa gatatcgaag
    14521 attttaaatt ccaagccgaa ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa
    14581 aagctgatct gccagagtat cctgaacaaa tataataatg attattttct caacagattt
    14641 aactatataa gtgagaaaag tttgcgtatt tcctaggaat gtgatgaaat tttgaatttt
    14701 gtttgaaact accacgacaa cgtttttaaa ggaaatggga tatggtatag ttaatatata
    14761 aaaataataa ctaattacag gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc
    14821 agctactagt gcgttggctg ttttaactct agcagcttgt tcaggcgata ctaataaaga
    14881 tatcgcaacg atgaaaggcg gaacgatcac tgtatctgat ttctatgatg aagcaaaact
    14941 tgaatcatcg aaccaatcct tagtacaacg gatgatcatt tataaagtat tcaataacaa
    15001 atatggcgat aaagtaaccg ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc
    15061 attaggtgat acttttgaat cacaattaga agctgctgga tatacgaaag atacttataa
    15121 agaatatatt cgtaacaatt tagcttttga agctggcttg aaagctcacg tagatattac
    15181 agatgacgat ttgaaaactg cttggaaatc tttccatcca gaagtagaag cacaaatcat
    15241 caaattatca agtgaagatg aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga
    15301 tttctctaaa ttagccaaag acaaatcaac tgatacagag acaaaagaag atggcggtaa
    15361 agttaaattc gactcaacga caacaactat tcctgcagaa gtaaaagaag cagctttcaa
    15421 attgaaagac ggtgagatct ccgatgttat tactacaaca aatcctactt cttatgcgac
    15481 agaatactac gttgtcaaaa tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata
    15541 caaagatcaa ctaaaagata tcgctactga aactaaacta agtgacaatg cattcacaac
    15601 taaagtaatc ggtgaagaat taaaagatgc aaatgtaaaa atcaaagatg atgcctttga
    15661 aaatgtgttg agtgcattca caacaacttc atcaagtacg aaagactcaa gtgaaactac
    15721 agcttcaaca aagagttctg acacaaaatc aactgattca acaaaagaat cttcaactga
    15781 agaaacaact gattcatcaa aataaaagaa aacaaaaaaa gcgaagactt atgtctcgct
    15841 ttttttg
//
LOCUS       DASJON010000048        15616 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJON010000048 DASJON010000000
VERSION     DASJON010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.048"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..1429
                     /locus_tag="U3989_001149"
     CDS             290..1429
                     /locus_tag="U3989_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN1714608.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1496..2071)
                     /locus_tag="U3989_001150"
     CDS             complement(1496..2071)
                     /locus_tag="U3989_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1714609.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2076..3050)
                     /locus_tag="U3989_001151"
     CDS             complement(2076..3050)
                     /locus_tag="U3989_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN1714610.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3074..3118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3333..4301
                     /locus_tag="U3989_001152"
     CDS             3333..4301
                     /locus_tag="U3989_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1714611.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4414..4692
                     /locus_tag="U3989_001153"
     CDS             4414..4692
                     /locus_tag="U3989_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1714612.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4810..5046
                     /gene="secG"
                     /locus_tag="U3989_001154"
     CDS             4810..5046
                     /gene="secG"
                     /locus_tag="U3989_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN1714613.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5149..5904
                     /locus_tag="U3989_001155"
     CDS             5149..5904
                     /locus_tag="U3989_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1714614.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5907..8264
                     /gene="rnr"
                     /locus_tag="U3989_001156"
     CDS             5907..8264
                     /gene="rnr"
                     /locus_tag="U3989_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN1714615.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8286..8750
                     /gene="smpB"
                     /locus_tag="U3989_001157"
     CDS             8286..8750
                     /gene="smpB"
                     /locus_tag="U3989_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN1714616.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9052..9771
                     /gene="atpB"
                     /locus_tag="U3989_001158"
     CDS             9052..9771
                     /gene="atpB"
                     /locus_tag="U3989_001158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN1714617.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9824..10039
                     /gene="atpE"
                     /locus_tag="U3989_001159"
     CDS             9824..10039
                     /gene="atpE"
                     /locus_tag="U3989_001159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN1714618.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10130..10654
                     /gene="atpF"
                     /locus_tag="U3989_001160"
     CDS             10130..10654
                     /gene="atpF"
                     /locus_tag="U3989_001160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN1714619.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10641..11183
                     /locus_tag="U3989_001161"
     CDS             10641..11183
                     /locus_tag="U3989_001161"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN1714620.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRMQLEKMRNQLNR"
     gene            11206..12762
                     /locus_tag="U3989_001162"
     CDS             11206..12762
                     /locus_tag="U3989_001162"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN1714621.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12776..13678
                     /locus_tag="U3989_001163"
     CDS             12776..13678
                     /locus_tag="U3989_001163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN1714622.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13701..15107
                     /gene="atpD"
                     /locus_tag="U3989_001164"
     CDS             13701..15107
                     /gene="atpD"
                     /locus_tag="U3989_001164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN1714623.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15121..15543
                     /locus_tag="U3989_001165"
     CDS             15121..15543
                     /locus_tag="U3989_001165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN1714624.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5170 a         2825 c         3384 g         4237 t
ORIGIN      
        1 tttttgcaac tagcttctgt atatgatgtt tacaaagaat ggtacaagaa aagatcatcc
       61 ttgaaaaagg aaagctaaca ataactagca tcatataaga gcttttaagg atagaacagt
      121 agaataggaa tctatgggca agtcctctcg tataaataaa ataggaggga agtagcagca
      181 tatttttcat aaagtggctc tctttcttca tttttacttt ggttgctcct atttgaaaat
      241 tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga gaggattaga tgaaaaaaga
      301 agaaaaaatc gcgattttac aagagattat ccgaattaaa tcagtgaatg gaaacgaagg
      361 tgaagtagca gcctatttga ataagctttt ggcaaggcat gacataacag gagagattgt
      421 ttcttacaga gatggtagag ataatttgat tgctcgttac caaaagggcc aatcaggaaa
      481 agtgttaggc ctatctggac atatggatgt cgtggcggct ggtgatgaat cgtcttggac
      541 ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg tacggacgtg gtgctacaga
      601 tatgaagagt ggcttggcag caatggtgat tgccatgatc gaactgaaag agtcaggaaa
      661 accatttaac ggaacagtca aattattagc cactgtcgga gaagaagtgg gtgaactagg
      721 tggggaacag ctcacgaaag caggctatgt agatgactta gatgccttga tcatcggaga
      781 gccaaccaat tacagtttga tgtatacgca catgggatca atcaactata cagtcacctc
      841 gcacggaaaa gaagcgcata gctctatgcc agaccaaggt tataatgcaa tcaatcacct
      901 aaatgaattc attacgaaag caaatgctga aatgaatcac ctcgctgaaa caatcgagaa
      961 tcctgtccta ggaaaaacaa tccataatgt tacattgatc agtggcggga atcaagtcaa
     1021 cagcatccca agccatgcgc agctccaagg gaacattcga tcgattccag aatacccaaa
     1081 tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa ttgaatcaag agacagacta
     1141 tcatttggaa ttgatgatcg attacaacaa aatacctgtc aaagctgatc cagattctcc
     1201 tttgattcat tccattcagc aacaattcag ccagccatta cctttagttg gagcggcagc
     1261 aacaacggat gcagccgaat ttacgaaagc taaccattcc ttcgactttg tcgtctttgg
     1321 gcctggtgtc gttactttgc ctcatcaagt agatgagtat gttgaaatcg ataattattt
     1381 ggatatgatc gaaaaatatc aaggaattat tttatcctac ttagcgtaag atttttttaa
     1441 taaatgaaaa aacagagcca gattagcatt attttgctaa tccagctctg tttattcagc
     1501 ctatttttct ttttgaatat atcaagcgtt ggggcaacat agtgatgact aatgaaacaa
     1561 cgacgataga caacaagcga tcaaggtagt ctgtacccac ctgtaccaag atcacacttg
     1621 ttgtctgact taaacctaac ccatgtaata gctgaacgat catactggat cctgaggagg
     1681 tgatcccgcc aaataactga acggtaatga tcgaagaaac gatcgttcca ggtaccgaca
     1741 aaatcaaagc gagtaataac agtttcaagc tgtttttatc tttgccgaga tatcttggca
     1801 aaatcaatcc agcaagcaag cctgtgagaa tttggattgg cgaataatac agagaaaaaa
     1861 tatctgtagt cactccactt aaaataccgc tggacaatcc tgtcagcatt ccaggtacag
     1921 gcccaagcaa tgcaccagca aaaatcgttc cgatagaatc gagatagatt ggcaaacgaa
     1981 gccataaagc aatcgttcct ccaatataat tgatcccaat acacaaagcc atcactgtaa
     2041 gtacgcgagt tgccattttg tttttattca ttttgttagc ccatctttaa cctttccaaa
     2101 tctttcataa tcgtttcttc ttgtgcattt aaaagaacgg ttaaaaactt tcggaaaaaa
     2161 agacgagtat ctacttttgt cataatttta ctgttagcag gtttctgcca aaattcataa
     2221 cgatcaacca atgtttgtcc gcgacttatt ccttgagttt ctacagcagt gaagctttta
     2281 aagccttcac agatggactc atcgataaaa taagcaacag ctaatggatc attgatgaca
     2341 catcctaaga ttctttcttg tttccagtga aagtcaaaat aaaaacgagt gatcgctttt
     2401 agatactctc cctcttcagg attcatttgg cagcaatatt caaggatagt cggtgtcaag
     2461 acgatttctc ttgtcacatc taacccaacc atttctatcg gttgtttgag cttttcgaat
     2521 acataagaag ctgcttcagg atcacaccag taattgtatt cagcaactgg tgaacagtta
     2581 ccatggcttt tgtaagttcc gcccattgag acaaaacgat ccatatgttt cccaacagat
     2641 gggctgtttt tcaaagcaga agcgatattg gtcaaaggcc ccaaagcaat gactgatgta
     2701 tctgtttttt ctttgaaata atcagtcaga aaatcaactg catggagcga ttctgcctgt
     2761 ttggctaaga ccatcgggaa atttgtgtcc cctaatccat ccaacccatg agtgtcttgc
     2821 gcactaatga aaggttgctt caaaggtttt tccattcctt gataaacagg gatatctaaa
     2881 cgatcacagc gctccaaaca tttcagcaca ttctgcgtac cgatatctac agggacattc
     2941 ccgcaaacga cggtaatagc aactacttcg atatcagggg atttgacagc tagtgataaa
     3001 gctaaagtgt cgtcgatccc tggatcgcaa tcaataatta ctttacgcat tatttccaat
     3061 ctcctttctt tagagatact tagtttttta ttctgggaag tttccgaacc agtccaagac
     3121 gagtgtcttg tttataatgc acgaaattag tatactggct tttattgtga atgtaaatgc
     3181 tctcttacaa gtaaatctat tgagaagaac tttaagtaaa tgatacaatg aaaaagaaat
     3241 ggaatttacg tgtgccgaag aaaaagaatc ctattgatcg gtatattgta taataaatag
     3301 attatagttt ggcagaaagc ggcgggtgta cgatggttat tttttatttc attcttattc
     3361 tgatatttat aggaatcagt taccattttc tttcttattt ttatgcaaag aaattagtaa
     3421 gagcgggggt acagagaggc tatgactggt atgaagaaac tgggcaccgc ttgatggatc
     3481 ctgctgcagt cacgtcagta gaaaaaaagc gacaaaaatt agaagaagag tgtgaaagct
     3541 tctgggaaca aggaataccg aaaacgatca aaagttatga cgggttgaaa ctagctggac
     3601 agatgttcct ccaaccaact cagcagaata aatgggtcat ttgtgtacat gattatcgaa
     3661 gtacagggaa aagagatatg tctcatatag gtaaaagata tgcagagaaa ggattcaacg
     3721 ttcttatacc cgatttacgt gcgcatggag aaagtgaagg agaaatcatc ggaatgggat
     3781 ggctagaccg gctagattta attgcatgga tccaactcat cttagatgaa caaccagatg
     3841 catccattat tttgcacggc agttcgatgg gcgcttctac tattatgatg gcaagtggtg
     3901 aaaagcttcc atctgcagtc aaaggattta tcttagacag cggatatgtt tctgtttatg
     3961 cggaatttcg gtatatgtta agtaaaatca cggtctttcc taaaaaaatg atcatgagat
     4021 atgcgaatca ctatgctcag aaatacgcag gttattcttt aaagcaagca tcagctacaa
     4081 gacagcttgg tagtaaccat ttgcctttgt tagtcatcca tggtgaaaga gatcattttg
     4141 ttcctacaga agcagcatac acgattcaaa acgctacggc aggggataaa gcattactgc
     4201 ttgttccaga agcagaacat ttggaagcat cgatgaaaga tccgaaaacc tattggacag
     4261 tgattttttc atttattgaa caaagaataa cgatggattg attttctctt cctttttgta
     4321 agcaacacga ggataaataa ttgtttatag accatgagac tgttaaaata aggtaggaat
     4381 aaaaaaatat cagttatttg aggagtgcgg catatggaaa tcaaagagga aaaaaatcgt
     4441 cttgctttat tcaatgatga gcaacaagaa atcggagaaa tgacatggtc ggatgcgggg
     4501 ccagacatca tgatcattga ccatacattt gtcgatccag catatcgtgg tcaaaaatta
     4561 gctgaaaagc tagtgtatac tggagtagaa ctagcaagac gagaggggaa aaagattatc
     4621 ccttcatgcc cttacgcaaa aaaagagttt gagaagaaac cagagtatca tgacgtctta
     4681 cgccaatcat aataataaca gctgaaagat ttgcactttc agctgttttt ccatttatat
     4741 agagttgtca cggagtttta tctatgataa agttaaagag acgatatctt ttgggaagga
     4801 ggctgctata tgtataattt aattttggga atcgtgatcg ttttatcgat tgttatggtc
     4861 attgcgatta tgatgcagcc aagcaagcaa aatagtgcag cgagtgcatt taccggcggt
     4921 gcggatcaac tattcggcaa acaaaaagct cgcggctttg aagctgtcat gcagcgatca
     4981 actgcagtca tgggagctgt ctggatgatc ttattatttg tcctagcatt tttatcttcg
     5041 aagtagtcga ataagcctct cgcacaacgg cgggcggctt tttttattgc aaagagaacg
     5101 cttaagaaga agttatgaaa gaaactatgg taaaatgagg cgaaagaggt gaaacgagtg
     5161 aaacaattac caaaacccct ttatgcaaaa catggaaaac gagcagtttt gctgcttcat
     5221 gcgtactcag gaagtccaaa tgatgtccgg atgctcgcac ggtttttaga aaaagcagat
     5281 tatacagtct atgcgccgct atttaaagga catggaacga tggagccata tgatatcttg
     5341 caagaaaaag cagaaagctg gtgggcagat actaaaaaag ctatccattt tttacagtct
     5401 gaacagtttt ctgatattgc cgtattggga ttatcgatgg gtggcatctt tgctgtacgg
     5461 gcactagaag aagaatcagt aattggcggc ggttttttct gttcgcccct gtctcctgta
     5521 gaaacaaacg tgcctgaaaa ttttgaaaaa tatgtacgtc aagtgctgaa gatagcaggc
     5581 aaatcagaaa aagaaatcaa tgaaaaagct gtgtcttacc gctcgttagc tgaacatcaa
     5641 ttaatggata tacaagacca agcagcaatc gcagaaagta gactatcaga tatccaacag
     5701 ccaatatttt tagctcaagc tgggaaagat gaaatgatcg acccaaatgg tgtatttgaa
     5761 acggcacgca aattgagccg ccaacgagtg acacttcaat ggtatcctga aagcggtcat
     5821 gtgattacag ttggcacagc aagacgcgaa ttagaaaaag acgtgttgga atttttagaa
     5881 aaattacctt ggaatgagga ataaatatga caaaagaaac aataaaagag aaaattctct
     5941 ttttcatgga aagcagtcgc aaacgtagtt tttctatgga agaaatcgct gaaggtctag
     6001 gctttcaaaa aagcgatgat tttaaattac tagtacaaac gattgctcaa atggaaagag
     6061 aacagagtat cgttttcaat aaaaaaggaa aagtcaaact tcctttaaaa ccgatcttgg
     6121 tcgaagggac atttcgggca aatgaacgag gatttggatt tgtaacgatt gatcccgaag
     6181 aagatgatat ctatatcccg aaagaagcga ctggctttgc aatggatggg gataccgtag
     6241 caattgatat catgaagcga gcaaatacag atatggatcg cggagcagag ggaaaagtag
     6301 tggagattcg agaccgagcg actactcagt tagccggaga atttgttgct tattcagacg
     6361 aagagataca ggaaacggat ctttatggtg tggtcatccc gaaagataga aagcttaacc
     6421 aattcaaagt gtatgcagcg gcagagggag ttcgtccagt tgacggaagt attgtattga
     6481 tcgaactgac ccattaccct gaaaaagggt atgcaactag tttagaaggg ataatcaagc
     6541 aagtgattgg tcataagaat gaccctggca tggatatcct ctcgattgtc gtggctaacg
     6601 gtattcctac aaaatttcct gatgatgtgt tatctgaggc agatgaagtt ccagaccaga
     6661 tcagcgaaaa ggatattgtt ggacgcagag atttaagaga tcagctcatc gttacgattg
     6721 atggagaaga tgcaaaagat ctagatgatg cagtgacggt caaaaaatta gaaaatggta
     6781 attattttct tggagtacac attgcagatg tatcttacta tgtgacgcaa ggcagtcaac
     6841 tggacagaga agcatacgaa agaggaacaa gtgtctattt gaccgatcga gtgattccga
     6901 tgatcccaca acgtttatcc aatggtatct gttcattgaa tccccatgtt ccacgactca
     6961 ccatgagttg tgaaatggag atcgatccaa atggacaagt tgtctctcat gagatttttc
     7021 caagtgtgat ccaaacaaca gaacggatga cctacacagc tgtcaatcaa atcttagaag
     7081 atcaagatga agaaacgatg caacgttatg ctcagctcgt gcctatgttc caagaaatgg
     7141 gtgaattgca tcgaatcctt gaaatgatgc gaatcaaacg tggagcgatt tcttttgagg
     7201 atcgtgaagc gaagatcctt gttgatgctg aaggacaccc aaatgatatc cagctaagaa
     7261 gcagagggat aggtgaacgc ttgatcgaat cctttatgct tgctgccaac gaaacggtag
     7321 ctgaacatta taataaatta aatcttccgt ttatttatcg aatccatgaa cagccaaaag
     7381 aagaaaaaat gcagcgcttc tttgattttg cttcagcttt gggcattctg gtccgaggaa
     7441 cgaaaggaac gatcacacca aaagatttac agcatgtgat cgaaggagta gaagacaaac
     7501 cagaagctgc agttatcaat actatgttgc taagaagcat gcaacaagca cgctattcag
     7561 aagacaattt tgggcattat ggcttagcgg cagattacta cacacacttt acatctccta
     7621 ttcgacgcta tcccgatcta atcgtccatc gtctgatccg ttcttacagt gaagataaaa
     7681 gtgaaagcaa tcaaagttcg tgggctgcgg aacttcctga aattgcagat catagttcta
     7741 agatggaacg tcgtgcagta gaaacagaac gtgaagtaga ttccatgaag aaagcagaat
     7801 tcatgatgga caaagtggga gaagaattcg atgggatcat cagctctgtt gtgaaattcg
     7861 gcctgtttat cgaactgccg aatacagtag aaggattgat ccatatcaat gaattgaaac
     7921 aggactactt ccattttgtt gaaaatcatt tagctcttgt aggtgaaaga actggtctga
     7981 cttttaaaat cggacaaaaa gtgcgcgtaa aagtagtcaa agctgatcca gatgaacgag
     8041 cagtggattt tgaactcgtt tccgcagaag aggtcactgc gttggaacgt ccaaaagcac
     8101 gcaatggaaa atctggaaaa cgagaaggaa aacaagcagg caaacgctca gaaaaaaatg
     8161 gcaagaagaa caaacgatat agtgatagta agcaaacgtt cggtaagaaa aaagggaaaa
     8221 agccatttta caaaggtgtc aaaaagaaga aaaagaaaaa ataaaatgag ttggaggaaa
     8281 cagctatgcc aaaaggcgag ggaaaactaa ttgcacaaaa caagaaagct cgccatgatt
     8341 attcgatcat cgacacgatg gaagcaggga tggtcttgca aggaacggag atcaagtcga
     8401 tacgaaacag ccggatcaat ctgaaagatg gatttattcg cgtccgcaac ggggaagctt
     8461 tcttgcataa tgttcatatc agtccttatg aacaaggaaa tatttttaat catgatccgt
     8521 tgcgcacgag aaagttatta ttacacaaga aacaaatcgt tcgtcttgaa aacgaattga
     8581 aaaacactgg aatcactgtt gttcctttaa aagtctatat tcgtaacggc tatgccaagg
     8641 tattgattgg tctggcaaaa gggaaaaaat cgtatgataa acgggaagat ttgaaacgaa
     8701 aagatatcga tcgacaaatt gatcgaacat taaaaaattt ctctagataa tttcattatc
     8761 ctttttcgca tattcggtgc ttttgctttt acttaatgat aaaatttgtt agcatttaat
     8821 ctgataaaac atgattatca caggtttttt cttagatcag gagcaccgtg catggggaaa
     8881 aattcgcttc gtttttgact gaaataaaaa agtgtctaat tttcatgaaa atgttttgaa
     8941 ttttctgttt catggtggta tgataccgtg tggttagaaa ccgattggaa aaaaccagat
     9001 acgcacatga attgatttta tgtgaatttc ggggaaaaga ggtgaaatgg attggatgaa
     9061 cgctcgctaa cgtttcacat tggaccaatt tggtttgatg gtacagtttg tataatggtg
     9121 ttactgacct gtttgattgt tttcttctta gcttactttt ttacaagaaa tcttaagttg
     9181 aaaccaacag gcaaacaaaa tgcgatagaa tgggtcattg attttagtcg aggaatcgta
     9241 acagataatc tgcctagaaa agaattgagc aatttccatt tgttggcatt tacattgttt
     9301 ttatttgttt ttgttgcaaa caacataggt ttagtaacaa aaatcgtctt acctaacgaa
     9361 acaacacttt ggaaaagccc gacagcagac ccgtttgtta ctttaacttt ggcatttata
     9421 atgatcttac ttactcactt attcggggtg aaaaagttag gcttcaaagg atatttcgta
     9481 aattcgttct tgaaacccta tagttttatg tttccgatga aactgattga agaatttaca
     9541 aacttattaa cacttgcttt acgtctttat gggaatattt atgcggggga agttttgcta
     9601 accttgattg caaaaatgat gacaagctta ggctggttct cattaccatt agcaattcca
     9661 ttagagatgg tctggatcgc gttctccttg tttattggga gcatccaagc atttgtcttt
     9721 gtcactttgt caatggttta tatgagccat aagattgaag tagaagaata atactcggtt
     9781 aaaaaacaaa ctactattta tctattttag gaggaaaaca attatgaatt atatcgcagc
     9841 agcaatcgca atcatgggag cagcaatcgg agccggttat ggtaacggtc aagttatctc
     9901 taaaacaatc gaatcaatgg ctcgccaacc tgaaatgtct ggtcaattcc gtacaacaat
     9961 gtttatcggg gtagctctag ttgaagctgt tcctatctta ggggtagtta tcgccttgat
    10021 tctagttttt gccgtttaat tgaatcagta ttaaggcaga aacaacatcg tttgttactg
    10081 cttttttaca tggaaactag aaatactcca aagaaaggaa ggctcgccta tgctgaatca
    10141 attggcaatt gcagaagttg gcaatgcgat gttaggtaat atcatcgttg tcagcggctc
    10201 atttttgata ctactggctc tcttgaagca ctttgcttgg ggaccaatca gcgatatttt
    10261 gaaaaaacgt gaagacaaga tcgccaatga tttagattct gcagaacaat ctcgcatcaa
    10321 ctcagcgaaa atggaacaag aacgcgaaca acaattgtta gcctctcgtt ctgatgcagc
    10381 tgatatcatc aaaaatgcga aagaaagtgg agaattaagc cgccaaaata ttttgaagga
    10441 tgctcaagaa gaagcagctc gtctaaaaag caaagcccaa gctgatatca ctgtagaacg
    10501 tgattcagcg ctgaactctg taaaagacga cgttgcagaa ctctctcttc aaatcgcgga
    10561 aaaaatcttg aacaaagaat tatctccaga aatgcatgaa tcattaatca atcaatacat
    10621 tgaaggtcta ggttcttcaa atgaaactag ataaatatac agtaggaaga cgttacggga
    10681 aagctttatt tgaactggcg atcgattcaa atcaagcaga agaaatctat caagatctcg
    10741 taagtttacg tcaaatctat gaacaagtac ctggattagg gaatatgtta agtgatgttc
    10801 gtctagaacc tcatgaaaaa cgagaaatca tggacaagct tgtcagcggt tatgatggaa
    10861 tcgttaaaaa ctttttagaa gttgtttatg agtataaccg aatggatgac cttttgttca
    10921 tgattgaaga atatgaacgt cgatatgatg aacatcaagg gttactattg ggaagtgtca
    10981 caacagcagt cccactttca gacgaacaac gaatacaatt agaaaagaat gttgcaaaaa
    11041 caatggacta tcaatcagtt gaattaaaac aaatcgttga ttcttctatt attggtggcg
    11101 cgattgttga agccaaccat cgagtaatcg acggaagcat tcgcatgcaa ttagaaaaaa
    11161 tgcgtaatca attaaacaga tagtacaaag aggtaggtga atcgaatggc catcaaagca
    11221 gaagaaatta gtgccttgat taaagaacaa attgaaaatt atcaaaacgt gctttcagtc
    11281 gaagaaatcg gtaccgtaac ttatgtaggt gacgggatcg ctcgtgcaca tggtttagaa
    11341 aacgcgatga gtggtgagtt gcttgaattt tcaaacggct catacggaat ggcgcaaaac
    11401 ttagagtcaa atgatgtagg tatcattatc cttggcgatt tcgagtccat tcgtgaagga
    11461 gataaagtaa aacgaacagg taagatcatg gaagttccag ttggagaggc cttgatcggt
    11521 cgggtagtca atccgctagg tcaaccaatc gacggactag gtgaaatcgt tactgacaaa
    11581 gctcgtccag tagaagcgat ggcaccaggt gttatgcaac gtaaatctgt taacgaacca
    11641 atgcaaacag ggctaaaagc gatcgacgcc cttgtgccaa tcggacgcgg acaacgtgaa
    11701 ttagtcatcg gtgaccgtaa aacagggaaa acttctattg cgattgatac gatcatcaac
    11761 caaaaaggtc aagatatgat ctgtatctat gtagcaatcg gacaaaaaga ttctacagtt
    11821 cgtacacaag ttgaaacatt gaaaaaatat ggcgcaatgg attacacaat cgttgtgaat
    11881 gccggtgcgt ctcaaccagc accattactt tatatcgcac catatgctgg tactgcaatg
    11941 ggtgaagaat tcatgtacaa cggtaaacat gtattgatca tttttgatga tctttcaaaa
    12001 caagctgttg cttatcgtga actttccttg ctacttcgcc gtccaccagg tcgtgaagct
    12061 tatcctgggg atgttttcta cttgcactca cgtttattag aacgtgcagc aaaattaagt
    12121 gatgatctag gcggcggttc aatgactgct ttgccatttg ttgaaacaca agctggagat
    12181 atctctgctt atatcccaac aaacgttatc tcaatcactg acggacaaat cttcttagaa
    12241 agcgacttgt tctacgcggg tactcgtcca gcggttgatg ccggactatc tgtctctcgt
    12301 gtagggggtt ctgcgcaaat caaagcaatg aaaaaagttg ctgggacttt acgtctagac
    12361 ttagcaagtt atcgtgaatt agaagcattt acacaattcg gttctgattt ggatgcggca
    12421 acacaagcaa aactaaatcg tggacgccgt acagtagaaa tcttgaaaca aaaactgcat
    12481 gcaccgctag ctgttgagaa acaagtagtg attttgtatg ccttgacaca tggattcttg
    12541 gacagtatcc ctgtagacag tattttagac ttcgaacatg aattattcga atatctggat
    12601 accaatcatt cagatatttt tgaaacaatc cgtacaacaa aagatcttcc agaagaagaa
    12661 acgttgaaca gtgcgattca ggaatataaa gacatgttct tagctacaaa aggaaataat
    12721 tcctctacag aagataaatt gaaatccatt caaaatgcat agtgaggtga gatgaatggg
    12781 tgcttcatta aatgaaatca agacacgtat cgcttcaacg aaaaaaacga gccaaatcac
    12841 gcgggcaatg cagatggtgt ctgcttctaa gttgacaaaa tctgaagctt cttcacagaa
    12901 gtttcaggtt tatgcaacaa aagtaagaga aatcgttacg catttgactg ctactcagtt
    12961 aaatgatatt gcctcagaaa atcctagagg ggatatcaat tacaacagca tgctgctcag
    13021 ccgtcctgtg aagaaaaccg gttatatcgt catcactgca gatggcggtc tggtcggtgg
    13081 atataacagt tcgatattga aacaaacgat gtctatttta gaagaagacc accaatcacc
    13141 cgatgattac gtgatgatcg caattggcgg aactggtgca gacttcttta aagcacgcgg
    13201 tatcaaccta gcatatgagc tgcgcaattt atctgatcag ccaagctttg atgaagtccg
    13261 caaaatcgta aatatggcaa caactatgta ccaaaacgaa gtattcgatg aattatacgt
    13321 atgttataac catcatatca attcgctaac tagtcagttt cgtgtagaaa aaatgctgcc
    13381 aatctcagac ttagatccag aagaagcaac gactttcgaa caagaatata tttttgaacc
    13441 ttcaaaagaa gaaatcttgt ctcagttatt gcctcagtat gctgaaagtt tgatctacgg
    13501 tgcgatcgtt gacgccaaaa cagcagaaca tgctgctggt atgacagcaa tgaaaacagc
    13561 aactgataat gctgctaata tcattgatga gctgacggtt tcttataacc gtgcaagaca
    13621 aggggctatt acccaagaaa ttaccgaaat tgttgccgga gcttcggcac tagaatagta
    13681 ttgagattgg aggaaaaaag atgagttcag gcaagattgt tcaagtaatc ggtcccgttg
    13741 tcgacgtgga attttcttta gaccaatcct taccagatat caacaacgca ttagttgttt
    13801 ataaaaatga cgaaaataaa tcaaaagttg ttcttgaagc agcgttagaa ttaggggacg
    13861 gagtgatccg gaccattgcg atggaatcaa cagatggttt acaaagagga atggaagtca
    13921 ttgatacagg caaagcaatc tctgttcctg taggtaaaga aacattaggt cgcgtattca
    13981 acgtactagg agatacgatc gatttagaaa cacctttccc agaagatgcg gaaagaagcg
    14041 aaattcataa aaaagcacca gcctttgacg aattaagtac aagtacagaa attttggaaa
    14101 cagggatcaa agttatcgat ttgcttgccc catatttaaa aggtgggaaa gtcggactat
    14161 tcggtggtgc cggtgttggt aaaaccgtac tgatccaaga actgatccat aatatcgccc
    14221 aagaacatgg tggtatttct gtatttaccg gtgtaggtga acgtactcgt gaaggtaatg
    14281 acttgtatta tgaaatgaaa gattcaggag ttatcgaaaa aacagccatg gtgttcggac
    14341 aaatgaacga accaccaggt gcacgtatgc gtgttgcttt gactggtttg acgattgcgg
    14401 aatatttccg tgatgtagaa ggtcaagatg tactgttgtt tatcgacaac attttccgtt
    14461 tcactcaagc tggatctgaa gtatcagcct tgttaggacg gatgccttct gcggttggtt
    14521 atcaaccaac attggcaaca gaaatgggtc aattgcaaga acgtatcaca tctacgaaaa
    14581 aaggttcgat cacatcgatc caagcaatct atgtgccagc cgatgactac actgacccgg
    14641 cgccggcaac agcattcgcc catttggatg cgacaaccaa cttggaacgt aaattaacag
    14701 aacaagggat ttatccagcg gtagatccat tggcttcttc ttcaagtgct ttagcacctg
    14761 aaatcgtggg agaagaacat tataaagtag caacagaagt ccaacacgta ttgcaacgat
    14821 atcgtgaatt acaagatatc attgctatct taggtatgga tgaattatcc gatcaagaaa
    14881 aagtgttagt ttctcgtgcc cgcagagtcc aattcttctt gtcacaaaac tttaacgtgg
    14941 ctgaacaatt tactggtctt ccaggttcat atgtccctgt agaagaaaca gtgaaaggat
    15001 tccgcgaaat cttggaaggc aaatacgatg atcttcctga agaagctttc cgaagtgttg
    15061 gccgaatcga agacgtcgta gaaaaagcga aaacactagg ctactagaaa ggggtgcccc
    15121 atggatcaat atttaacagt aaatgtggta actcctgccg gtttggtcta tgatcaccat
    15181 gcagctatcg tcgttgcacg tacgacagct ggtgagcttg ggattttgcc taaacatgca
    15241 ccaatcatcg ttccattgac gattgatgaa gttcgtgtaa aacgaacaga ttcagatacc
    15301 cacgtcgatt ggatcgctgt taatggcgga atcatggaag ttcgtgataa tgttgtttca
    15361 atcgttgctg atagtgcgga acgtgaacgt gatatcgatg tatctcgtgc ggaacgtgca
    15421 aaacaacgcg cagaacgcca aatcgaagaa gcaaaagcaa aacaagatac gaacgaattg
    15481 aaacgggcaa ctgttgcgtt gcaccgagca atcaatcgta tcaaagtatc taagcactca
    15541 taaaatgaga tgcggatgtt tatccgtgtt taagaacagt aaaaagaggt tatgacagaa
    15601 gtggtcagct tcaaga
//
LOCUS       DASJON010000049        15315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJON010000049 DASJON010000000
VERSION     DASJON010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.049"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="U3989_001166"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="U3989_001166"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN1714625.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="U3989_001167"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="U3989_001167"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN1714626.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="U3989_001168"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="U3989_001168"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN1714627.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="U3989_001169"
     CDS             complement(3374..4681)
                     /locus_tag="U3989_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN1714628.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="U3989_001170"
     CDS             complement(4684..5265)
                     /locus_tag="U3989_001170"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN1714629.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="U3989_001171"
     CDS             complement(5837..7129)
                     /locus_tag="U3989_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1714630.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="U3989_001172"
     CDS             complement(7126..7749)
                     /locus_tag="U3989_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714631.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="U3989_001173"
     CDS             complement(7752..8588)
                     /locus_tag="U3989_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1714632.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="U3989_001174"
     CDS             complement(8605..9492)
                     /locus_tag="U3989_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1714633.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="U3989_001175"
     CDS             complement(9508..10791)
                     /locus_tag="U3989_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1714634.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="U3989_001176"
     CDS             complement(11474..12157)
                     /locus_tag="U3989_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1714635.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="U3989_001177"
     CDS             complement(12175..12792)
                     /locus_tag="U3989_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1714636.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="U3989_001178"
     CDS             complement(12802..13626)
                     /locus_tag="U3989_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN1714637.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="U3989_001179"
     CDS             complement(13619..14179)
                     /locus_tag="U3989_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714638.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="U3989_001180"
     CDS             14353..15309
                     /locus_tag="U3989_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1714639.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4495 a         3417 c         2622 g         4781 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaa
//
LOCUS       DASJON010000050        15293 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJON010000050 DASJON010000000
VERSION     DASJON010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.050"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..120)
                     /locus_tag="U3989_001181"
     CDS             complement(<1..120)
                     /locus_tag="U3989_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose pyrophosphatase"
                     /protein_id="HEN1714640.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKR"
     gene            348..1010
                     /locus_tag="U3989_001182"
     CDS             348..1010
                     /locus_tag="U3989_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN1714641.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            1134..2330
                     /locus_tag="U3989_001183"
     CDS             1134..2330
                     /locus_tag="U3989_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN1714642.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(2431..3807)
                     /locus_tag="U3989_001184"
     CDS             complement(2431..3807)
                     /locus_tag="U3989_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1714643.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(3844..4710)
                     /locus_tag="U3989_001185"
     CDS             complement(3844..4710)
                     /locus_tag="U3989_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN1714644.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(4799..5824)
                     /locus_tag="U3989_001186"
     CDS             complement(4799..5824)
                     /locus_tag="U3989_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1714645.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(5842..6516)
                     /locus_tag="U3989_001187"
     CDS             complement(5842..6516)
                     /locus_tag="U3989_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1714646.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(6700..7593)
                     /locus_tag="U3989_001188"
     CDS             complement(6700..7593)
                     /locus_tag="U3989_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1714647.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(7667..9364)
                     /locus_tag="U3989_001189"
     CDS             complement(7667..9364)
                     /locus_tag="U3989_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1714648.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(9357..10670)
                     /locus_tag="U3989_001190"
     CDS             complement(9357..10670)
                     /locus_tag="U3989_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN1714649.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(10670..14707)
                     /locus_tag="U3989_001191"
     CDS             complement(10670..14707)
                     /locus_tag="U3989_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1714650.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(14720..15178)
                     /locus_tag="U3989_001192"
     CDS             complement(14720..15178)
                     /locus_tag="U3989_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1714651.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4342 a         3435 c         2648 g         4868 t
ORIGIN      
        1 tctcttcgaa gttcctccat ctggcaaacg aacttcgtct acagcaacat caataatttt
       61 tccgctatag atttctttgc gactgattgt tttttcttca aattgctcat tgttcagcat
      121 atcttctcct gctttctttg taatcataac tgcttttttt tcatttttca atttttctca
      181 aaaactcctt attagtttac catagataaa ccttagattt tctctaaata tagaaaatcc
      241 ttctttagtc cgatactctt tctagtagtc cgtatgctaa aataagtatg taatcgaata
      301 acgaaaagaa ttcaatactc aacgataaat gacatggagg gtttattatg ataaaacgaa
      361 attggccgtg gcttctagca ttggtcttgc tatttctctt tgtccgaaac aatagttttt
      421 cttttgttct aggactttta ttgataggtg tagttttagt tttgtgggga ctctttggtt
      481 cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact gactgacgaa cttgaaactc
      541 actatgaaaa aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc
      601 aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag
      661 caatcgatct ccgccatgat ccagtcaaag cagcgaaagc tttattcaaa gaattggtga
      721 aagaaccaaa ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag
      781 ttgatttgac aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct
      841 atgaaaaact agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag
      901 attatcaaaa attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga
      961 aacagagctt aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt
     1021 cataaaagaa gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa
     1081 gtagagacat tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc
     1141 ataaaccgac tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga
     1201 acaacccgtt ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc
     1261 agctccaaga gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag
     1321 aacaagctaa acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct
     1381 acggatcggc tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc
     1441 aagcacaaga tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag
     1501 aagccaatcc tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga
     1561 aggtcaaaca atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg
     1621 acaagatttc agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt
     1681 tagaacagct ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg
     1741 ctggagaatt gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag
     1801 cagaagaaac tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag
     1861 atcgtctgga taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag
     1921 caccacaaat ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat
     1981 ctatcgctac agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc
     2041 gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga
     2101 agaaaaattc agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg
     2161 gaatcgtgga tatcgagacc cttcaaacca ctcaaaatga tttgatcgaa acaatccaag
     2221 agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg
     2281 gacacatgga agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc
     2341 gcttgttact aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc
     2401 ttatctttct tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata
     2461 gccatagcca aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt
     2521 cacagtttgc agacgaagca tcaatacaat acaagtcaag atggagacga ttggcaatag
     2581 cggaacgaat ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt
     2641 cagtaagcct agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc
     2701 taatacattc aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt
     2761 cgcttttttc ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc
     2821 atccttgctg acagcaaaca gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt
     2881 cagtagcgtt aaaattgccc ctacagcgat caccgcgccc actgttccat gtcctacata
     2941 cctcattgca aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt
     3001 caagatcagc gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc
     3061 tcttggcaca tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa
     3121 tcccaagaaa gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg
     3181 catgaatggc tgccagttat caggtttaac gaaaaatatt cctacaatga taaacaacgc
     3241 gatgatacca aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc
     3301 aagcgacacg atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt
     3361 accattttcc ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt
     3421 caaaaaacct tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa
     3481 ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag
     3541 ataggcatat ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc
     3601 agacaaaatg acaacgattg cagctaagac aaatgaaagt gacagtgctg gaccagcatt
     3661 ttgattggcc gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc
     3721 aagcatgatc aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt
     3781 gatctctttc ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag
     3841 cgattaaact gttttcatat accctaaaag gataccacct aaaatgacta aaaggcaacc
     3901 aacaataaca taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc
     3961 taaagtagag ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga
     4021 tttgactgtt agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt
     4081 tttccacaca aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat
     4141 cattccgata ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat
     4201 gatggtatat acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa
     4261 atcaagcatt ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata
     4321 cgcaccgctt acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt
     4381 ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga
     4441 caatggtagc cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc
     4501 aacactccaa gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg
     4561 actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt
     4621 ttgttgattg gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa
     4681 catagggact aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag
     4741 aaacttatca gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct
     4801 aacgtttttc attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc
     4861 cttcagttga ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat
     4921 aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct
     4981 tatcaaagat ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa
     5041 ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat
     5101 atttgatggc attactaagc aattggttga agataaaagc gatccattta gcatcggtca
     5161 aaacagtatg atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg
     5221 cctgactgcg tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt
     5281 cccgcgagaa actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt
     5341 ttgataattc attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac
     5401 gaactagcaa tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat
     5461 aatctttttg gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga
     5521 tcagctgatt gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat
     5581 aattttgcaa aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc
     5641 tcaagtaacc acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag
     5701 caattgtgct caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg
     5761 caataaaaaa cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat
     5821 tcatcttaac cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat
     5881 atatccttgg atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa
     5941 tgtattgtca tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact
     6001 taagatcttt ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa
     6061 atcaatgatc tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt
     6121 gtggctcatc atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt
     6181 gatttttgca atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat
     6241 gttcatagcc atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac
     6301 tttcgatact tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat
     6361 atctagtaat acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc
     6421 ggtcgttcca aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat
     6481 gatctcttcg tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct
     6541 tgctactaca atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat
     6601 tttagcaaac gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag
     6661 cagcttaaat ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg
     6721 ttgtttttgt cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa
     6781 tatacctaga aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt
     6841 tgaagagact tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt
     6901 cacggtgtaa ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa
     6961 tgtatttgtt tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg
     7021 accatacact tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag
     7081 ttttttctca gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt
     7141 tggaaaagag gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc
     7201 atcaaataaa ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg
     7261 ttcgtaatat tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt
     7321 gacttgtccc attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg
     7381 cactgctaat tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt
     7441 ctcgatcctt ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa
     7501 aactgcagct ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg
     7561 ggtttgaggt ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac
     7621 tgcagcaacc tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact
     7681 tcattttaga tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta
     7741 agaatgccaa gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta
     7801 ctctttctgg ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat
     7861 aagagatgat aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg
     7921 ccatggtctc tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat
     7981 ttccatctgc gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc
     8041 ctgattcata aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt
     8101 cttcattcca caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt
     8161 tttcaaactt tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat
     8221 caaattcatc atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt
     8281 ctagttctgt ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg
     8341 ctaatctacc taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga
     8401 caaacccgtt gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc
     8461 cggctcgcac ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga
     8521 ttttgtaatt tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa
     8581 taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg
     8641 tttcttgttc tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat
     8701 tcatctgttt atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt
     8761 atgtgtcttt ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg
     8821 gcatcataac aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta
     8881 agaaaagata gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga
     8941 ataccgcttc tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta
     9001 aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac
     9061 cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta
     9121 cgtcaaacaa atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca
     9181 atccgctatt cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg
     9241 tcacttcact tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa
     9301 tccctattcc cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat
     9361 tcattttttt ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt
     9421 cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct
     9481 gattgacctg gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg
     9541 gtctcgttat aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat
     9601 tctaattcag ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc
     9661 acccaaaatt cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct
     9721 tccacacctt ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc
     9781 gtaacaaggc cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca
     9841 ttgtctgctg gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa
     9901 tatttccccc cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct
     9961 ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg
    10021 acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa
    10081 attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact
    10141 tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta
    10201 ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt
    10261 accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg
    10321 ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt
    10381 tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc
    10441 tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca
    10501 tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat
    10561 tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca
    10621 agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc
    10681 cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga
    10741 atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt
    10801 caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt
    10861 ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa
    10921 cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt
    10981 ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact
    11041 tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat agggaagaat
    11101 cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt
    11161 tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa
    11221 gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga
    11281 tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa
    11341 acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg
    11401 gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat
    11461 ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca
    11521 aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg
    11581 aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt
    11641 ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc
    11701 ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt
    11761 gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt
    11821 catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt
    11881 tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa
    11941 ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc
    12001 tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga
    12061 tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat
    12121 tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt
    12181 ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag
    12241 aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga
    12301 ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt
    12361 tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg
    12421 gtccagcgat ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa
    12481 tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg
    12541 gttcggcttc cccactctct ggattttggt catataatgc aaattcgact ccagccaacg
    12601 gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa
    12661 tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt
    12721 tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa
    12781 gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt
    12841 tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg
    12901 cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc
    12961 ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat
    13021 taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga
    13081 tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta
    13141 cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc
    13201 gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt
    13261 tctcttgata cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact
    13321 tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt
    13381 gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat
    13441 cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag
    13501 tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat
    13561 cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa
    13621 tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat
    13681 ttctattttc tccgccattg tctttataca atgtttcata gccattttta agccctagtg
    13741 taagcggagt tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac
    13801 gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag
    13861 ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga
    13921 gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat
    13981 tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga
    14041 ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg
    14101 ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt
    14161 ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc
    14221 aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat
    14281 tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga
    14341 tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg
    14401 tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt
    14461 cgttttcaac ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc
    14521 caagacgctc caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc
    14581 tgggtacgat tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag
    14641 cggaatcttc tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa
    14701 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    14761 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    14821 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    14881 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    14941 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    15001 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    15061 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    15121 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    15181 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    15241 tctaattata atttaattta attttaattt taaatcaaat gatatccttt tta
//
LOCUS       DASJON010000051        15200 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJON010000051 DASJON010000000
VERSION     DASJON010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.051"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            248..1021
                     /locus_tag="U3989_001193"
     CDS             248..1021
                     /locus_tag="U3989_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1714652.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="U3989_001194"
     CDS             1018..1785
                     /locus_tag="U3989_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1714653.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="U3989_001195"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="U3989_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN1714654.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="U3989_001196"
     CDS             3750..4070
                     /locus_tag="U3989_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN1714655.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="U3989_001197"
     CDS             4070..4420
                     /locus_tag="U3989_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN1714656.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="U3989_001198"
     CDS             4587..5525
                     /locus_tag="U3989_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN1714657.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="U3989_001199"
     CDS             5540..6370
                     /locus_tag="U3989_001199"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN1714658.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="U3989_001200"
     CDS             6414..7439
                     /locus_tag="U3989_001200"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1714659.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="U3989_001201"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="U3989_001201"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1714660.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="U3989_001202"
     CDS             8517..8957
                     /locus_tag="U3989_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN1714661.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="U3989_001203"
     CDS             8960..9562
                     /locus_tag="U3989_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1714662.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="U3989_001204"
     CDS             complement(9625..10728)
                     /locus_tag="U3989_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1714663.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="U3989_001205"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="U3989_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN1714664.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="U3989_001206"
     CDS             12294..12689
                     /locus_tag="U3989_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714665.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="U3989_001207"
     CDS             12689..12940
                     /locus_tag="U3989_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714666.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="U3989_001208"
     CDS             12983..13378
                     /locus_tag="U3989_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714667.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="U3989_001209"
     CDS             13375..14631
                     /locus_tag="U3989_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEN1714668.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="U3989_001210"
     CDS             14643..14966
                     /locus_tag="U3989_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714669.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..>15200
                     /locus_tag="U3989_001211"
     CDS             14959..>15200
                     /locus_tag="U3989_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714670.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQ"
BASE COUNT         5215 a         2578 c         3217 g         4190 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga
//
LOCUS       DASJON010000052        14861 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJON010000052 DASJON010000000
VERSION     DASJON010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.052"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..723
                     /locus_tag="U3989_001212"
     CDS             22..723
                     /locus_tag="U3989_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN1714671.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            720..1841
                     /locus_tag="U3989_001213"
     CDS             720..1841
                     /locus_tag="U3989_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN1714672.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1856..3088
                     /gene="pepT"
                     /locus_tag="U3989_001214"
     CDS             1856..3088
                     /gene="pepT"
                     /locus_tag="U3989_001214"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN1714673.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3220..4128
                     /locus_tag="U3989_001215"
     CDS             3220..4128
                     /locus_tag="U3989_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN1714674.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4163..4435
                     /locus_tag="U3989_001216"
     CDS             4163..4435
                     /locus_tag="U3989_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN1714675.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4446..5492
                     /locus_tag="U3989_001217"
     CDS             4446..5492
                     /locus_tag="U3989_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN1714676.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5743..8352
                     /gene="clpB"
                     /locus_tag="U3989_001218"
     CDS             5743..8352
                     /gene="clpB"
                     /locus_tag="U3989_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN1714677.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8503..9189)
                     /locus_tag="U3989_001219"
     CDS             complement(8503..9189)
                     /locus_tag="U3989_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714678.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9167..9661)
                     /locus_tag="U3989_001220"
     CDS             complement(9167..9661)
                     /locus_tag="U3989_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1714679.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9681..10901)
                     /locus_tag="U3989_001221"
     CDS             complement(9681..10901)
                     /locus_tag="U3989_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1714680.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11048..12883
                     /gene="lepA"
                     /locus_tag="U3989_001222"
     CDS             11048..12883
                     /gene="lepA"
                     /locus_tag="U3989_001222"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN1714681.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13079..13990
                     /locus_tag="U3989_001223"
     CDS             13079..13990
                     /locus_tag="U3989_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1714682.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         4974 a         2578 c         3093 g         4216 t
ORIGIN      
        1 ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt agccgcagtg
       61 ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca cgcttactta
      121 cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa
      181 ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga ccgtatcacc
      241 gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac agctgtcacg
      301 attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa gttgaatcaa
      361 cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg tacattacga
      421 tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga agaaaataaa
      481 aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta caatgaaatg
      541 gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg
      601 acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa
      661 gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt tttaaagcca
      721 tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct cagtggctag
      781 ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca atccaacgag
      841 taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg
      901 atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg gttactgatc
      961 agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc tatgcagcac
     1021 acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa cttctcggta
     1081 ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa ctggccgttt
     1141 atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct ggtgctggta
     1201 cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga tttactccgg
     1261 aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt caagaggcaa
     1321 aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc atgcggtcag
     1381 ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca gtcgaaatgt
     1441 ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca tttgtagaac
     1501 aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa aatgcaaaat
     1561 cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat ccgcaagcaa
     1621 tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg gctcatgata
     1681 tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg ttatgcaagc
     1741 aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat attaactttt
     1801 tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga atactatgta
     1861 cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac
     1921 aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga
     1981 attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat tcgttatggg
     2041 aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt cccacatgga
     2101 tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact atgatggcga
     2161 gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag attttccaaa
     2221 tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat tactgggagc
     2281 agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga tcaataatcc
     2341 ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg
     2401 agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag tcgatggtgg
     2461 tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata ttacgatcca
     2521 agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc tacagttagc
     2581 aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg aaggatacga
     2641 aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta tgagttatat
     2701 cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca cacatatcca
     2761 agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt atgatcaata
     2821 ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc
     2881 aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa cagatggttc
     2941 aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag aaaatatgca
     3001 cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa
     3061 aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt
     3121 cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg gaattggttg
     3181 tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct gctagttttt
     3241 tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt tcaagattat
     3301 gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc atcgattgat
     3361 gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt aattataggt
     3421 ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc tcaataccat
     3481 gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga gattcccctc
     3541 aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat agactatggt
     3601 caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc agatacagca
     3661 gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat caagaagttt
     3721 tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga cgaggagcat
     3781 tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg cggttttaca
     3841 aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat cgtaccttcc
     3901 ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa attctatacg
     3961 atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct
     4021 gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt atctgttgta
     4081 tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa atagtagtta
     4141 acagaaagtt ggaagaagag taatggatgt attgttacac aatcctttgt ggttttacct
     4201 tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc aggctagaac
     4261 tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag gcggtggttt
     4321 gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga agtttactat
     4381 aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc attaaggagc
     4441 aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt ttgctgattg
     4501 tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc tttgccgaat
     4561 tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca gtttaccaaa
     4621 tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat tttacaacga
     4681 aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg tttggtacag
     4741 gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta tcccagggag
     4801 caggggtgac agcactccat atgattatgt acaaactggc agtgatcact tatgcattag
     4861 ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg atctggttta
     4921 tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg atgtccgtca
     4981 gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa agtgagcgtt
     5041 tgcggaaatg ggtggataca tgcaatcagc agatctactc tttaagggaa acggtccaaa
     5101 ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg ttaaaacttg
     5161 tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa atagattttc
     5221 tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt cctacgcctg
     5281 ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt gttggaacag
     5341 ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg ccatttctct
     5401 atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa ataatagaag
     5461 taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac cctttttatc
     5521 aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc tttttttata
     5581 tacatacaat caattttgtt tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa
     5641 ctatgtgtat attttgacca aaattgacca aagcgatata atagtttttg taaagatcaa
     5701 agaaataagt gatcttacct ttaattagga gggtatacct gtatgaatat tgagaaaatg
     5761 actacgacgc ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat
     5821 caagatatcg acattgccca tgtatggaaa atctttttac aatctaatca ttttggtcga
     5881 aatttctata cggatgccgg attggatgtc gaatcttttg aacatgaaat tgatcgtcta
     5941 ctggatgagt atccagttgt ttctggtgga aacgtccaat atggacaaaa cctcagccag
     6001 aatttctttc gattattgaa tgaagcagac caaatacgtg aaagcttcgg cgacgagttt
     6061 ctttcaacgg aagtcgttat tttagcgttg atgaaattaa aaaattatcc gttaactgtt
     6121 tatttgaata aaaacggatt atctgaaaaa gaattgcgta aaaacatcga agagatgaga
     6181 ggaggagaaa gagtgacctc acaaaaccaa gaagaacaat acaaagcatt agaaaaatac
     6241 ggcgttgatt tagtacagca agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat
     6301 gaagaaatcc gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg
     6361 ataggagaac ccggtgtagg gaaaacggca atcgttgaag ggcttgctca acgtatcgta
     6421 cgcaaagatg tgcctgaaaa tttaaaagat aaaacgattt tttcattaga tatgggtgca
     6481 ttgatcgctg gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa
     6541 gaagtcaaaa aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca caatatcgta
     6601 ggggcaggaa aaacagaagg aagtatggat gccggaaatc tgttgaaacc aatgcttgct
     6661 cgcggtgaat tacatttgat cggtgccaca actttagatg agtaccgtca atatatggaa
     6721 aaagacaaag cgttggaacg tcgctttcaa aaggtattag tcaaagaacc aacagtggaa
     6781 gatacgatca gtattctgcg tggattaaaa gaacgttttg agatccacca tggtgttaac
     6841 atccatgaca acgcattagt agcagcagct actttatctg accgttatat tactgatcgg
     6901 ttcttaccgg acaaagcaat cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa
     6961 atgaactcta tgccaacgga attagatcaa gttactagac gtttgatgca gttggaaatc
     7021 gaagaagctg cactgaaaaa agaatcagat gatgcaagta aaaaacgctt gaaaaatttg
     7081 caagaagaat tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca
     7141 gaaaaagaag aagtcaattc tgtttcagca aaacgtgctg aaatcgataa agcaaaacac
     7201 gaattagaag atgcagaaaa taactatgat ttagaacgcg ctgctgtatt gcgtcatggt
     7261 acgatacctc aattagaaaa agaattgaag gaattagaag ctaaagcaaa agacagcgag
     7321 attaagatgg ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt tggcagatta
     7381 acgggtatcc ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat taaattgaat
     7441 gaaacacttc ataaacgagt gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg
     7501 gtaattcgtt cacgggctgg attgcaagac ccaaatcgtc ctctaggttc tttccttttc
     7561 ctaggaccaa ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt
     7621 gactcagaag atcatatggt acggatcgat atgagtgaat atatggaaaa acatgctgtg
     7681 tctcgtctag tcggagctcc tccaggctat gtaggttacg aagaaggtgg tcaattaacg
     7741 gaagctgtca gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat
     7801 cccgatgtct tcaatatctt gttacaagtc ctagatgacg gacgcttgac tgattcaaaa
     7861 gggcgagtag tagatttcaa aaatacggtt ctgatcatga ccagcaatat cggttcacag
     7921 ttactgctgg aaggcgtgac tgctgatgga acgattccag aagcagtggc tgaacaagtc
     7981 aacacgctat tacgtggaaa cttcaaacca gaattcttga accgaatcga cgacacgatt
     8041 ttatttacac cattgagcct agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa
     8101 ttggcacaac gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg
     8161 tggattgctg aaaatgctta tgaaccagca tatggtgcaa gaccattgaa acgatttatc
     8221 actaaggaag tagaaacacc actagctaaa gaaatcgttg caggacatgt tatgcctaaa
     8281 tcaaaagtaa cgattacttt acttgatgga caacttcatt tcaaaacaga agagctagaa
     8341 gaaatcgtct aatcttccat tgagactata aaagaaaaaa ggggtctgac acaactagtg
     8401 tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga aaaataagcc
     8461 gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat ttttcacttt
     8521 ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc atgcaaacaa
     8581 tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc
     8641 tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa
     8701 gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc
     8761 aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa
     8821 gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat
     8881 tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat
     8941 gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt
     9001 caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt
     9061 gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac
     9121 gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag
     9181 tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt
     9241 ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag
     9301 ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag
     9361 tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc
     9421 cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt
     9481 ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt
     9541 cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta
     9601 actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca
     9661 tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg
     9721 atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa
     9781 taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg
     9841 gattttccta taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg
     9901 aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct
     9961 gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa
    10021 tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt
    10081 ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat
    10141 aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca
    10201 gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct
    10261 gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat
    10321 aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca
    10381 actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg
    10441 attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata
    10501 ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg
    10561 ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc
    10621 acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt
    10681 tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt
    10741 tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca
    10801 gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc
    10861 ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt
    10921 agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa
    10981 actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg
    11041 agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat
    11101 catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca
    11161 cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga
    11221 acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga
    11281 aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc
    11341 tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga
    11401 agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt
    11461 catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga
    11521 cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat
    11581 tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc
    11641 accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa
    11701 tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg
    11761 caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt
    11821 tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg
    11881 agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg
    11941 aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga
    12001 tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga
    12061 aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga
    12121 tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc
    12181 cgttatttac catgttaata aaacggatgg tacgactgct acagtagata acccggcaga
    12241 ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat
    12301 ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt
    12361 cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc
    12421 tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt
    12481 ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa
    12541 tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg
    12601 aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat
    12661 ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa
    12721 cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca
    12781 aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt
    12841 catggccgtt ctgaaaatgg atgagcaaga tagtgtgaaa taacttataa tcaatcgaaa
    12901 aacctattaa ataaaccttt ttgagtagat gcaacactga ggattatctg tataaaagat
    12961 tttaaactgt catttggtta tcacggcgat actaaaatta acaaaattgg aatatttttc
    13021 ttgaagattt ttagctgtca gtatatgatg aaagcatctc ataggaggtg cttttttcat
    13081 ggttgataat tcaagtggaa gggttcttaa atctctaaga aaagagaaaa aactatctca
    13141 aaaaaagtta gctgatttag caggtatttc acaaagtact ttagttaaat atgaaaaagg
    13201 gtcaagaaaa atccctaaag acgtagataa tactctaagc aagattctga atatagaaac
    13261 tctaataaaa gatgaggaag ataagattga gattctcatc ggtaaactaa ttgcatacag
    13321 agatatgaat aagttactta acaaagagtt agctgataat ataggaatta gtgaagtatt
    13381 actcagttat gtgttaaata gaaagagaaa tccgtcaaaa gaaatgcaaa aaaaaatcga
    13441 tatttttcta ttaagtaatg aaaaagaaat tttgaaggag attaatcgtg atagtgagat
    13501 atttagttta tcaaaagacg ataaaattgt tatgggaaaa agaatacgag aagtccgaaa
    13561 gaaccgggaa gagacgcttg aaaaatttgg taaaaacttt accatctata caggcaaaaa
    13621 tgttataagt agatgggaaa aaggtattaa tattccagat attgaaaaac taatgaatat
    13681 tgcctatctt ggtaaggtca ctgttcctta ccttatgtac ggagaagatt ataagaatat
    13741 acttccaaaa gatgaaagag ttagcgattt taaaaagata aattctttta gtatgggatt
    13801 aaggatgagg aaaattagga aagattatta tctagaaagg gaggagtttg gaaagctttt
    13861 ttctccttca atttcaaaat ggtccataga taggtatgag aatggtagag atatacccaa
    13921 tactaatcga attatccaat atgcttatat aggaaatctt tctttagaat ttttgattta
    13981 cgggatataa aaatagttta cgtcgatact tattaagtta tcgacgtttt ttattataga
    14041 aaaaatttat aaaaggaggt ttcttgatat gataagaaac aaaaagggtt tgttgaaagt
    14101 tgctttactg gcagcttttt tctaagtaac tttttgacaa gctcaatggc aacacaagcg
    14161 gaagaagtaa tacaagaact aactgttatg aataacagct agataaaatg ggctatgtat
    14221 gttatccaac aatttagtaa tcactagaag cctagcgaaa gttcgcttga taagttatag
    14281 atgagtattg atttgtagtg tttccaaatc aaaagggtac agagccacat gaaatatgtt
    14341 gtcctctgca gcgtcaggat gggaatccat accatggaga ttcggagatc tgtgagggaa
    14401 tacttacgtg gttaatatgt cagttgtgtt ggacgtagta atggtcatag tgggtagatt
    14461 gactgaattc ttatatgaga aaatgaaacc aacaagaaga tagggttatt tatacagaat
    14521 tggggatctt ttctggaaaa gtaatcggat aatgcgagat tcgtacggtc ttttttaaat
    14581 ttctctaaaa aaaacggcat cgaatgggtt gggtgattga taagcaatga atcatagact
    14641 gatatagaat aaaaaatgat tctatttatc tttttacatc tatcgttttt tcaagtagcc
    14701 gcaaattata tcagcattca acaatttttg tatacaagtt ctagtaaaag gatctgaaga
    14761 aaaagttggt tgttgtgaag ataaaaaaag aatcaggttt tactgagttt ttcttcaaaa
    14821 tttgtgttat taggttagga caaagataag aagaagtatg a
//
LOCUS       DASJON010000053        14548 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJON010000053 DASJON010000000
VERSION     DASJON010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.053"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            3..815
                     /locus_tag="U3989_001224"
     CDS             3..815
                     /locus_tag="U3989_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN1714683.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="U3989_001225"
     CDS             812..1612
                     /locus_tag="U3989_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN1714684.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="U3989_001226"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="U3989_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN1714685.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="U3989_001227"
     CDS             3109..4818
                     /locus_tag="U3989_001227"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN1714686.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="U3989_001228"
     CDS             5030..9388
                     /locus_tag="U3989_001228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN1714687.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="U3989_001229"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="U3989_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN1714688.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="U3989_001230"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="U3989_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN1714689.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="U3989_001231"
     CDS             11226..11519
                     /locus_tag="U3989_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN1714690.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="U3989_001232"
     CDS             11516..11827
                     /locus_tag="U3989_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN1714691.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="U3989_001233"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="U3989_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN1714692.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="U3989_001234"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="U3989_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN1714693.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4958 a         2583 c         3238 g         3769 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaat
//
LOCUS       DASJON010000054        14533 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJON010000054 DASJON010000000
VERSION     DASJON010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.054"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..829)
                     /locus_tag="U3989_001235"
     CDS             complement(134..829)
                     /locus_tag="U3989_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN1714694.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(919..2019)
                     /gene="ychF"
                     /locus_tag="U3989_001236"
     CDS             complement(919..2019)
                     /gene="ychF"
                     /locus_tag="U3989_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN1714695.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2028..2219)
                     /locus_tag="U3989_001237"
     CDS             complement(2028..2219)
                     /locus_tag="U3989_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN1714696.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2240..3130)
                     /locus_tag="U3989_001238"
     CDS             complement(2240..3130)
                     /locus_tag="U3989_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN1714697.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3117..3884)
                     /locus_tag="U3989_001239"
     CDS             complement(3117..3884)
                     /locus_tag="U3989_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1714698.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3896..4609)
                     /gene="rsmG"
                     /locus_tag="U3989_001240"
     CDS             complement(3896..4609)
                     /gene="rsmG"
                     /locus_tag="U3989_001240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN1714699.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4841..6742)
                     /gene="mnmG"
                     /locus_tag="U3989_001241"
     CDS             complement(4841..6742)
                     /gene="mnmG"
                     /locus_tag="U3989_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN1714700.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6759..8156)
                     /gene="mnmE"
                     /locus_tag="U3989_001242"
     CDS             complement(6759..8156)
                     /gene="mnmE"
                     /locus_tag="U3989_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN1714701.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8455..8838
                     /locus_tag="U3989_001243"
     CDS             8455..8838
                     /locus_tag="U3989_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714702.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9006..9764)
                     /locus_tag="U3989_001244"
     CDS             complement(9006..9764)
                     /locus_tag="U3989_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN1714703.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9782..10600)
                     /locus_tag="U3989_001245"
     CDS             complement(9782..10600)
                     /locus_tag="U3989_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1714704.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10597..10953)
                     /gene="rnpA"
                     /locus_tag="U3989_001246"
     CDS             complement(10597..10953)
                     /gene="rnpA"
                     /locus_tag="U3989_001246"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN1714705.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11071..11205)
                     /gene="rpmH"
                     /locus_tag="U3989_001247"
     CDS             complement(11071..11205)
                     /gene="rpmH"
                     /locus_tag="U3989_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN1714706.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11729..13063
                     /gene="dnaA"
                     /locus_tag="U3989_001248"
     CDS             11729..13063
                     /gene="dnaA"
                     /locus_tag="U3989_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN1714707.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13261..14391
                     /gene="dnaN"
                     /locus_tag="U3989_001249"
     CDS             13261..14391
                     /gene="dnaN"
                     /locus_tag="U3989_001249"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN1714708.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4030 a         3057 c         2597 g         4849 t
ORIGIN      
        1 accgtttatc gtaaaaaggg actgtgacaa gacttttgtc acagtccctt actattattt
       61 aactatggat aaacaaaatg aggttcttta ctacccctag atatctagca aaatagctga
      121 tctttttgct agtttatctt cttgggacgg ataaacttcc aataatcccg tattttttct
      181 tcaagtagta acggattgct aaagcagttg ccccgataat gataactact actggatcta
      241 atactggatt gataactggt ggcaatagtg ttgtcgcaga aaacagtacg acccaaagaa
      301 gtgtcgttcc acctaggata agcaatgtct tacccattcc tggtttcttg cttcgatctg
      361 ctccgggata ttcatattga tagatgtatt tgtacatcat ataaaagacc cagccaccga
      421 ttgcagaacc tagaacgagt gtagaaaagc cataagctgg cgttgttcca cgtgaaaaca
      481 atccggtaat cgcaatcatg atacttaata gaccaagcaa cagcaacgta ttatctaacc
      541 acatcaataa aggggtggat gaatttttta cttctgctgg cttattgatg attgcttccg
      601 ttctttcaga gactgtccca aacaattgac gggctgttct tccgcctttt tgttctgcaa
      661 ctaaagtagg cagaatgtca tgaagggctt ttactttttc ttcttcagac aaattcgctg
      721 cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt aattttgttt
      781 ctaaactatt gttttctttt acgattgctc gtaattgttc tggttccatt atctaatagt
      841 cctccttagc cgttcctttt ctcaacggtc gtcacaaaag acattctacc atattctaca
      901 gctagtttga tagccctttt acacattaaa gcggaataac attacgtcgc cgtcttgtac
      961 gacataatct tttccttcta atcgaacacg tccagcttct tttgctgcat gcatgtttcc
     1021 gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg ataaatccgc gttcgaaatc
     1081 tgaatggatg attcctgctg cttgtggtgc tttgattcct tttttgaatg tccaagcacg
     1141 tacttcttgt tccccagcag taaagtaagt agctaagcct aacaaatcat aagcagcacg
     1201 gatcaactga tccaaacctg attcttcgat tcctaaagct tctaaaaatt ccgctttgtc
     1261 ttcttcatct aattctgcga tttcttcttc tgcacgagca ctgaccacga tcacttctgc
     1321 attttcttct gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt ctgcatctgc
     1381 tacgtcatct tctgagacat tggctacata aagaattggt tttgtcgtca ataaaaatag
     1441 tgatttaacg attttttgtt cttcttcagt aaattcgatc gttcgggcag attttccttc
     1501 ttccagtaca ggtttgattt tatccagtac agctagttct gccactgcat ctttatcttt
     1561 ggttttagca acttttgcta cacgagtata gcgtttgttg atagattcta agtctgctaa
     1621 cactagttcc aagttgatcg tatcaatatc ttctagtgga tcaacacgtc cttctacgtg
     1681 agtaatatta tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta cttggcggat
     1741 atggcttaag aattgatttc ccaaaccttc tcctttactt gcgcctttta cgattccagc
     1801 aatatctgta aattcaaaag tcgtcggaac agtttttttc ggatgtacaa gttctgttaa
     1861 gcgttgcaaa cgccagtcag gtacttctac catccctacg ttggggtcga tcgtcgcaaa
     1921 gggatagttt gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag ttgattttcc
     1981 tacgttgggc aacccaacaa ttccagcagt caatgccata aatttattca ctctttctct
     2041 gcattttttt caattacttt ttttaatttc ttttcaaaat ctcgacgggt catcatgacg
     2101 atatgtccgc aattcataca tctgattttg atatctgcgc ccattcgaat gacttcccag
     2161 cgattcgtct ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa gtcgtacata
     2221 aaaatagcct cctttttgtt tattcttcat caaattgaat attcaagata tcaagaattc
     2281 gagtcaaatc tttttgagaa agatattcaa tctcgatctt acctttccca tttttttctt
     2341 ggatagctac acttgtacca aatttatcca tcaaacgatc ttcgctttct cggatataat
     2401 atggtttttc ctttgctagc cttgggattt ttttcttttc ttttccatca ttttgattta
     2461 actcagaaac aagctgttcc agttggcgga cagttaaatt ttcagttact gcacgattgg
     2521 ctaatttcaa gatcaatgtt ttatttttta aacctaacag cgtacgggct tgtcccatag
     2581 ataaacgctg atcttgtacc atttccttga caagatccgg taaagaaagc aagcgcaaat
     2641 aattggcgat ataaggtctg ctctttccta aacgactagc tacttctgct tgtgttagtt
     2701 tcaggttttt catcagcatc tcatatgctt ctgcttcttc taacggattc agatcttctc
     2761 gctgcaagtt ttccagcact gctacttgca tcatcgcttc ttcgtcaaag tctctaacga
     2821 tagcagggat cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc tcccctgcaa
     2881 taatctcata tccttttact gcggaacggc gtacgatgat gggttgaaag acacctgatt
     2941 gttcaataga attagctagt tcctgcaaag atacttcttc aaatgtttta cggggctgat
     3001 acggatttgg tcgcagctca ttcaatggta gctggacaac ggtatcatct tttacatcca
     3061 ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct aatcctttac
     3121 ttttcttcac gacttaccac ttcctttgcc aatgcttggt agacttcagc tcctcgagaa
     3181 cgtggatcgt agtcgatgat cggttttcca tgactaggtg cttctgataa acgaatattt
     3241 cttggaatga tcgtttcgta aactttttcg cggaagtatt tacgtacttc ttctacgact
     3301 tcatttccca gatttgttcg tgcatcatac attgtcagta agactccctc gatctctaat
     3361 tctggattaa agtgtttttg gactaaacga accgtattca gtaattggct taatccttcc
     3421 aatgcgtaat actcacactg aacaggtatc aaaatcgaat cacttgctgt aaatgaattg
     3481 attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa tataatcata ctgactgctt
     3541 acttctgcta aagatccttt taatcttgat tctcttgcca tcattgacgt caattcgatc
     3601 tctgctcctg ctaattggag agtcgcaggc acaatactca aattttcgtg ttcagtgatc
     3661 aaagttgcct catcaattgg caattcattg actagaacat catagatgtc tcttgtcaca
     3721 tcaggttttc tgatcccgac accactggta gcatttcctt gtgcatccat atccactagc
     3781 aatacttttt tgccaaggct agcaagacaa gcacctagat tcaccgtcgt cgttgttttg
     3841 ccgacgccac ctttttggtt cgcaacagat attatttgtg ccatctcttc tcctcctatt
     3901 tgattggctg cttattaggc agtccgggct ttcttgggta tttctttggg gttggtttct
     3961 ttttttgaat aacgatgata tgccgttgat cagagacatt cggcaaggta accgcctctt
     4021 ctttgaggaa tttaccacct aataaagcaa tcgcttgttt tgcttctgtc aattcctctt
     4081 cactttttgc tgctttcaat gcataaaaat agccttcttg tttagccaag ggcaaacaca
     4141 attccgctaa cacattcaaa cgagcaaccg cacgagcagt gacaaggtca aatgatgcac
     4201 gaaagtgagg attttgccca aatgtttctg cccggtcatg atataaggaa gcatcgattc
     4261 ctaataatcg ggttaactca gataagaagg ttattcgttt attcagagaa tcaacgatcg
     4321 ttatctgcag ttctggaaag gcaattttta aaggtatgct aggaaaccct gcccccgaac
     4381 caacatcgca taaagataaa ggttgcgtaa agtctacttc aaatgctaat gtaagtgaat
     4441 cgtaaaaatg cttcagatat acgtcttttt catccgtaat agctgtaagg ttcatctttt
     4501 cgttccattc gactaatgtc cgataataaa tagcgaattg attcatttga tgatcggtaa
     4561 gctctatccc tttttctgcc aaggattggc gaaattcttc tggtatcata aatattcctt
     4621 tcattgtgaa acagcaattg tttcacagat tctctcccta ctttaacgca aaatctctag
     4681 acatgcaatc cttccatatg gattcttaaa aatttgaaaa gtaagaaaag ccgatagact
     4741 ttttctttct agtatgactc ataaaatata cacagaagag agaactagct ttgagcaatt
     4801 tctgcaaaaa ctagttctct cttctattta ttattacgta ttacgctggt tcactgactt
     4861 tcgcaatctt cccttgttcg atatagacca ttaggatact taggtctgca gggtttacgc
     4921 cactgattcg gctagcttgg gcaattgttt ctggttggat tttctgcaat ttttgttttg
     4981 cttctgttgc taatccgttg attgctgcgt agtcgatgtt ttctggaatc tttttcgctt
     5041 ccatgcgttt taatttttct actttttcca tggctttttt gatgtagcct tcatatttga
     5101 tttggatttc tacttgttcg atttcttttc gtgtcaattc ttcattttct ggaataaacc
     5161 gtagaagttc tgcataagta atctcaggac gtcttaaaaa gtcgcttgct agtacaccat
     5221 cttttaatgg tgccgcatta atcgttgcta aaaagtcttg gacttcttct gttggtttga
     5281 ttcgtacatt catcaaacgt ttgatttctt gttcaactgc ttcttttttc ttcaaataat
     5341 tcttgtattg atcttctttt actaagccta cacggtgacc catttctgtt aatcgcaaat
     5401 ctgcattatc atgacgtaaa agcagacggt attcagcacg tgaagtcagc aaacgataag
     5461 gttcgttcgt tccttttgtt accaaatcat cgatcatgac accgatgtaa ccgtcacttc
     5521 gtttgatcac taaaggttct tttccctgaa ctttcaaagc tgcattgata ccggcaatca
     5581 gcccttgtcc agccgcttct tcataaccgc ttgtaccatt tgtttgtcca gctgtataaa
     5641 gattttcaac gactttagtt tctaatgtcg gacggagctg gtgtggctct actacgtcat
     5701 attcgatagc atatcctgta cgcatcattt ctactttttc taatccagca atagaatgaa
     5761 gcatgttgac ttgaacatcc tctggtaaag aagtagataa accttgaaca tagacttctt
     5821 ccgtatttaa gccttctggt tccaaaaata attgatgacg tggtttgtct gcaaaacgca
     5881 cgatcttatc ttcgatggat ggacaatagc gagcgcctac cccttcaacg atccctgtaa
     5941 acattggggc acgatgtaag ttgtctcgaa taatttggtg agtcgtctca cctgtatagg
     6001 tcagccagca agattcctga tctagtttat aggcactatc tggtgtgctg aagctaaaat
     6061 gattcggctc tttgtcacca ggctgctctt ctgtaacaga atagtcgatc gtacttgatt
     6121 ttactcgagg cggtgttcct gttttgaacc gttcgatagc aaagccaagt tcttttagat
     6181 gattcgctag tccaatagaa ggttgagagt tattcggacc tgatgaatat ttcaattcac
     6241 cgataatgat ttctccgcga agtgctgttc cggctgtaat aacaactgct ttggcccggt
     6301 agctcgctcc tgtggacgtc actactccat gacaaacacc atcttcgacg atcagttctt
     6361 cgacgattcc ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt ttcatttctg
     6421 ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg gacagctggg ccttttcctg
     6481 tgttcaacat acgcatttgg atataggttt tatcgatatt cttacccatt tctccgccta
     6541 acgcatcgat ttctcgaacg acaacgcctt tagctggtcc gcctacagaa ggattacatg
     6601 gcatgaatgc taccatgtcc aaatttattg tcagtaataa tgtttttgat cccatacgtg
     6661 cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc acgtcatacg
     6721 catctgcttc ataatgttcc aatcgtttgt tcctcctttt attttcctaa acaaaattga
     6781 ctgaataatt gagtgattag ctcatcttgt acactgtcgc cgacaacttc tcctagatag
     6841 tcccagcaac gcgtcatatc tatttgaacg agatccacag gcattcctga atcaatacct
     6901 gcaatgactt cttcaagtga gagggaagct ttttctaaca acgcgatatg gcgggtatta
     6961 gaaacataag tagcgtctct ttctcctgtt tctccgctga agaataaatc agaaatagct
     7021 gattccaaac gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac tggttcattt
     7081 tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt tttatttaga
     7141 agaatgatcc gtttcaatcc acttgtcgct tccaataatt gttcatcttc tgctgtcagt
     7201 ggttcgcttt ggtttagaac aagtaagatc aagtctgctt ctgctaatgc ttttcggctt
     7261 ctttcgaccc cgatccgttc caccacatct tctgtttccc gaattccagc tgtatcgatt
     7321 agcttgagcg gtaccccccg aacattgaca tactcttcga ttacatctcg agtcgttcct
     7381 gcaatatccg taacgattgc tttttcttct cttaacaaat ggtttaacaa acttgatttg
     7441 ccgacatttg gccggccgat gattgccgta cttaaccctt cccgcagaac cttcccttgc
     7501 ttggatgtag caagtagcgc ttggattctt tgttggatca tctgcgcttt ttcaagcaat
     7561 aacttcgtag tcaattcttc gacatcatca tattctggat aatcaatatt tacttctact
     7621 tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga cagattgccg
     7681 tctaactgat taagagctag tcccattgct ttgtctgttt ttgcccgaat caaatccatc
     7741 acagcttctg cctgtgaaag gtcaacacga ccattcaaga atgctcgttt tgtgaattcg
     7801 cctggctcag ctaatcttgc accttctctt aacaatagct gcaaaatttg attgactaca
     7861 acgatcccac cgtgacaatt gatttctact acatcttccc gcgtaaatgt ctttggtgct
     7921 ctcatcacgg agaccatgac ttcatcgaca agctgattgc tcttgggatc aacgatatgt
     7981 ccataatgga tcgtatgact tggtacttct gataatcgtt tgtttccaga ttgataaact
     8041 ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa cgatactgat tgcaccttct
     8101 ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta aagtaatttc agccattttt
     8161 tcacttcctt tttcattttg cgcacaaaaa aagtgcccac tccacccttt atgcacaata
     8221 aacggtgaac ttcgcacttt gactgcttgt tcttgattaa attctggtca tagattatca
     8281 cacttgctat aaggaatcaa gtgtttaaaa caattagatg aagttgagcc tgttctgctt
     8341 ccattacctt atatttaatt gtccttggat gtctctttgc gttctttggt ttattcgaag
     8401 agaagatact gtataaacaa ggctttatct ttcttaaaaa aaacggtaca ctaaatggta
     8461 gcgctatcta aactatacac ttttgaaagg agaaaatatg tgagcccgtt cagatctgct
     8521 gcattggaag aagaaagtga ccctcggatc aacgatgtcg ttttatctac agggaatatc
     8581 aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga agctgatttg
     8641 tttgacccat cttttcccaa agatgacctt ttcttaacaa cgaagaggaa attaaagacg
     8701 aaggtacttg aatatggtgc gaatgcagta atcaactgtc attttgagta tcagcaaaaa
     8761 aaacgcgacg gaaagtattt tctggaactt tttgcttatg gaacagtgat ccaatttatc
     8821 caaacaacga ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg aagacagttt
     8881 cgatccgctt ttttctatca aagttagtga ctacttcatc gtatactctg ataagaatga
     8941 tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt caaaaagtaa
     9001 tggaatcaat aatatttttt agcaggttct actactaaat aacgataagg ttcctcacct
     9061 tccgaatgtg tttttacatg ttcatctttg cttaatgcag catggatttg tttacgttca
     9121 aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc gttttacttt ttcagccgtg
     9181 cgatcagcta aacgctgaat gatctcttgg cgcttttcac ggtaattgcc tacattgact
     9241 actacagaca gcttatttct tgctaaacga tggatgaaga cttgtgccag atattgcaat
     9301 gcattcagtg tttttccatg tttcccaatc agtatgcctt gcttttgtgt ttctaaatgg
     9361 aaaacaacca tattatttga ttctctttcc attttgacaa gagctggagc atcaagctgt
     9421 tttgaaattt cagtaagata aactgccaat tccgttaaag ctgcttcatc acctagctca
     9481 cttacttctt ctgttttttc ttcaagaatc gtttcgttga ctacctcgat ctcagctgtt
     9541 ggttcttctt ccaaaatttc ttctacagtc tgttctactg tttcagaaac tttttcgctg
     9601 atacttggct ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa accttttttt
     9661 ccttcttcca gtatttcgat ttcgacttgg tctttggcca aacccaaatc agccagacct
     9721 agttggacag cttcgtctat tgttgcagct tcataaatcg gcattagcat tacctcccct
     9781 attatttccg ttttttctta ggattcattg ctttcttaag agcacgttct ctttcgcgga
     9841 ttctccgtgc ttcttcttca cgctcttttc gaattttgaa agggttgttg atcagcagcg
     9901 tttgtacaac ttggaaggca ttggaaacta cccagtataa tgaaagaccg ctggctaaat
     9961 tgacacccat cactaaaatc attacaggca tcacgaaatt catgatcttc attgatgcat
    10021 tcgattcaat ttgactcatg ctagataaat acgtacttgc aaaagtaaag attgctgcta
    10081 aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt caaccataag aaatgccctt
    10141 gtttcaactc tggtacacgt gaaatagatt gccataacgc catcaagatt ggcatttgta
    10201 ctaataaagg aagacaacca gcatatggat tgacgccatt ttctgcatat aaacgctgct
    10261 gctcttctct taacttactt tgtgtttccg gatctcttga actatattgt tgctgcagtg
    10321 cttttaattt cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg aaatgcatca
    10381 acggcaatag gatcacgcgg atgatcagtg taaataaaat gatccctatc cctgcattac
    10441 caaatgacaa cgccttgatc gcctcagcaa agtaataaac aatataacga tcccaaattc
    10501 ctgtactctg tgcgctgact tccccagttc cgcaaccgga taagacgatg accaaactaa
    10561 cgataccggc catcaaaagt aaacgtttgt atttcttcac ttactctttt cctctctgtc
    10621 gattattttt gcaagtttta agacgtggac taaattggag cgaacctcat cataagtcaa
    10681 ccccttcaca cttggtctag caatcacaat aaaatccaac tccggatcaa tttcttcttt
    10741 tatagaataa actgctgctc ggatttttcg tttgacttca ttgcggttta ccgcgttgcc
    10801 gacctttttt cctacagata aaccaactcg aaaatgaggc tgtcccggtt tttccaaacg
    10861 gtaaacgacg aatttgcggt tagcaaaaga atctcccgta tggaacacgg cttgaaactc
    10921 tcgttctttt tttactcgat acgcttttct cattagtttt ctttcctttt ctatctctca
    10981 cttacgctca taatcatacc ataattacga ttgtcctttc atcatttccc taaaaaagag
    11041 tataaaaaaa accactgaaa ccccagtggc ctaagctgaa agaacttttc ttcctttgcg
    11101 acgacggcta gctaacacac gacgtccatt tttagtactc atacgtttac ggaatccgtg
    11161 aactttttga cgtttacgtt tatttggttg gtatgttctt ttcatatatt ccacctccaa
    11221 aatgttctta tctattttat aaacacagac atactttgat agtataacga caaagtagtg
    11281 attttgtcaa tataaaatag aagatcgatt gttaaaactt atccacaaga ttattttttt
    11341 ctatgggagt cctatgtgga aaaagttatg cacagggatt atccacagat aataaatggc
    11401 tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggt gatttcctgt
    11461 gtattccgtc tcctaactat tggtatatct atatttttta tccacagatg caagaggaaa
    11521 tctattgatt tttacgagtt ttccacctgt gcattagtgg tgtggaaaac ttttttttga
    11581 caactgtgtt tttctctttt ttctttttgt cgattgtgtg gataattgta attaaaaaat
    11641 ttttttcttc ggtttctgtg gaaaactatt gaaaaaaatg ctaaattagt actagttatt
    11701 cactttttaa tgaaggagga ggccttatat ggtatccctc gatgctttat ggaatgaatt
    11761 aaaagcaaca taccaaaagg atttatcgcc tgctagctat aatacatgga tcgaaacagc
    11821 gaaccctcga acccttgatc aaaaccagct cgttgttgag gttcctagca agattcataa
    11881 agaatattgg gaaaaaaacc tggcgacaaa aatcgttgaa gtcggttata tgttatcagg
    11941 aaatgagatt atccctcgct ttattactgg cgaagaagca gagcaagaag aagttataga
    12001 agaaaaaaat ccaaaagttg tggcacctag tccgctaaaa aaagccatgc tgaaccctaa
    12061 atataccttt gatacgtttg tcattggtaa aggaaaccag atggctcatg cagcagcgct
    12121 cgttgtagca gaagatccag gttctattta taacccgctc ttcttttatg gaggcgtggg
    12181 acttggtaag actcacttga tgcatgcaat tggtcatcaa atgctgcaaa gtcagcctaa
    12241 tgccaaagta aaatatgtaa gcagtgaaac ttttgctaat gatttcatca attcgatcca
    12301 aaacaaaaca gcagaagagt tccgtcaaga atatcgaaat gttgatttac tgctagtgga
    12361 tgacattcaa ttttttgcag aaaaagaagc tacgcaagaa gagtttttcc atactttcaa
    12421 tgccttgtac aatgaaggga aacaaatcgt actaaccagt gatcggttgc caaacgagat
    12481 tccgaaactg caagaacgcc tcgtttctcg ctttgcatgg gggctgtctg ttgatattac
    12541 accgccggat cttgaaacaa gaacagcgat tttacgtaaa aaagcggcgg ctgaacgtct
    12601 agagatccca gatgacacac taagctacat cgcaggacaa attgattcta acattcgtga
    12661 attagaaggt gcgcttgttc gagtgcaagc ttttgctgcg atgaatagtg aagatatttc
    12721 gactagtcta gctgcagacg cactgaaaac gttaaaatca ggaaaaggac atcctcaatt
    12781 gtcaatcctg cagatccaag aagaagtggc aaagtattat cacatccaat tgaaagatat
    12841 caaaggaaag aaacgcgtca aatctattgt tgttcctaga caaatcgcta tgtatttggc
    12901 acgtgagctc acagataatt ctttaccgaa aatcggagct gaattcggtg gcaaggatca
    12961 tacaacggtt atccatgctc atgaaaaaat ccagcagctg atggattcta gtgtcagtat
    13021 gcaaaatgaa gtatcagaaa tcaaaaactt tttactaaat taatttgtcg attctatcaa
    13081 ctgaaaatag catgtggata aaagaaaaaa acgttcaaaa gttatgcaca ctttttccac
    13141 agctgttttt tcgtttctct cttacgtttt ttgagttttc cacagaatca acatgcccta
    13201 ttacttttat tattcttttt aactaataat aaataaacaa acattcaaag gagatacatc
    13261 atgaaagtta ctttaaaccg agctagcttt atgcaggaat tgcaaactgt tcaacgagct
    13321 atttcaagca aaaccacgat ccctattttg acaggtgtaa aaatcacact gacacaagaa
    13381 ggtttgactt tgacggggag caacgctgat atatcaattg aaactttttt gtctgttgaa
    13441 aacgaaaaag caaatatgca aatcgaatct actggttcca ttgttttaca agcacgtttc
    13501 tttagcgaaa tcattcggag acttcctgaa gaaacattta ctttagaagt tttagaaaat
    13561 aaacaagtag cgattacttc tggaaaagcg aattttatcg taaatggatt agatgcagat
    13621 aactatcctc atcttcctgt tgtcgaaagc cataaccaga tgaaattacc tgtacacgta
    13681 ttgactaaac taatcaacga aacagttttt gctgtctcac aacatgagag tcgtccaatc
    13741 ttgacaggtg tgcatttcat tttatctgat aattctttat tggctgtagc tactgattct
    13801 caccgtctaa gtcaacgcgt gattccagta gaacaagcgg ctgatcattt tgatattgtt
    13861 attcctggaa aaagtttgat cgaattatct cgctcattaa caaatgaaga agaaatcgtc
    13921 gaaatcagca ttatggaaaa ccaagtgttg ttcaaaacag aaacgatgta tttctattcc
    13981 cgtttgctag aaggaaacta tcctgatacc aatcgtttga ttccttcaag ttttaataca
    14041 gaggttgaat tttctgttcc tagcttttta gcggcgatcg aacgggcttc tttactttct
    14101 catgaaggcc gtaacaacat cgttcgttta tctattcgtc cagatgcggt tgttttatac
    14161 ggaaattcgc cagaaatcgg gaaagtagaa gaaagcttga gttatacagc aagcagtggc
    14221 gatccattag atatttcttt caatccggat tatatgaaag cagcgttgcg tgcatttgga
    14281 gatatgagca tcaaagtgaa atttatctct gctattcgtc catttacatt ggaaccaacg
    14341 gaagatggcg ttcaatttat tcagctgatt actcctgtac gtacaaatta acaatgattt
    14401 tcattgttct tgaaaattta attgaaaaga aatagaaaaa ataaaatttt aaacagaagg
    14461 ccctaaaatc tcttttaagg ctttctgttt atttttgttt aaatcacctt ttttagatta
    14521 aatcgtctta tat
//
LOCUS       DASJON010000055        14478 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJON010000055 DASJON010000000
VERSION     DASJON010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.055"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(83..1501)
                     /gene="nhaC"
                     /locus_tag="U3989_001250"
     CDS             complement(83..1501)
                     /gene="nhaC"
                     /locus_tag="U3989_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1714709.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1904..3127)
                     /locus_tag="U3989_001251"
     CDS             complement(1904..3127)
                     /locus_tag="U3989_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN1714710.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3158..3997)
                     /locus_tag="U3989_001252"
     CDS             complement(3158..3997)
                     /locus_tag="U3989_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1714711.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4014..4694)
                     /locus_tag="U3989_001253"
     CDS             complement(4014..4694)
                     /locus_tag="U3989_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714712.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4696..5769)
                     /locus_tag="U3989_001254"
     CDS             complement(4696..5769)
                     /locus_tag="U3989_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1714713.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(5825..6063)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(6106..6339)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6416..7087)
                     /locus_tag="U3989_001255"
     CDS             complement(6416..7087)
                     /locus_tag="U3989_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1714714.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7174..9852)
                     /gene="mgtA"
                     /locus_tag="U3989_001256"
     CDS             complement(7174..9852)
                     /gene="mgtA"
                     /locus_tag="U3989_001256"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN1714715.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10511..10948
                     /gene="ndk"
                     /locus_tag="U3989_001257"
     CDS             10511..10948
                     /gene="ndk"
                     /locus_tag="U3989_001257"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN1714716.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10970..11836)
                     /locus_tag="U3989_001258"
     CDS             complement(10970..11836)
                     /locus_tag="U3989_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1714717.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(11996..14089)
                     /locus_tag="U3989_001259"
     CDS             complement(11996..14089)
                     /locus_tag="U3989_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1714718.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4204 a         3099 c         2460 g         4715 t
ORIGIN      
        1 taccgagaaa ctggcttttg aataccgttt attcgtaaaa gggattgtga caaaagtctt
       61 gtcacaatcc ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt actgcttctt
      121 gcggtttcac aaattttttt gagattcccc aacctgtgaa ggcactgacc atcgaaaaca
      181 caggagaaag caaactgaaa aatacgaatg gcaagtaagt gagtgtagga ataccaaatg
      241 tacttgctac gaaacttccg gcaattcccc aaggcactaa gtagttgatt actgttccac
      301 catcttctag cgttctacct aatacagttg gatcaaatcc tgctttttca taagtttctt
      361 taaatgcatt accaggcaga ataacagata ggaattgttc accaataaaa atatttactc
      421 cgatacttgt catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc tttcttgata
      481 ctcgttccat cacggttgag atcaagccaa attcgatcaa caatccacct aatgacaaag
      541 ttagaataat caatgacaca gtaggcatca tactttcgat tcctccgcgg ctaagcagtg
      601 aatcgatttg ttggtttcca gtatttgaaa caaaccctga ttcgatgacc ccagccaact
      661 tcgttaagga agtttgtggg ttttgaataa aaatcattcc tactgctaca gcgatattca
      721 acaaaatcgt taaaattgcc ggcacctttt tccatgcaca cagtaacatt aaaaataaag
      781 gaatcagtga ccagattgaa atagtaaagt ttgcttctag aacatcagtc acttcacgaa
      841 tagatgaaag acttgcggct ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa
      901 caacaaatgc cgggacagtt gaccacatca tatgcttgat gtgtttgaac aaatcggcat
      961 caacaatagc tgctgctaga tttgttgatt cggataatgg agacatctta tctccaaaaa
     1021 cggcacctga aacaatagct ccaacaacca gcgctggatt taagcctagt gttgtcccaa
     1081 taccgaaaaa tgcaatccca atcgttgaca taacagtaaa ggcactccct acagcgctac
     1141 ctacgattgc acacacaaca aatacagaag ggacaaacca ctggacactg atcaatttga
     1201 acccaatgac cattagagtt ggaatgatcc ctgcttgtat ccatacagca atcaatgaac
     1261 cgactaaaat aaaaataaag attgggatga ttcctggttt gatgccattg atgattcctt
     1321 cgtgcagcca atctgtcggg tatcttttta tcactgcata caacatgacc attccgatcg
     1381 ctgctaagat cgtgatatta ggtgagacct ttaatccgac aacgccaatc gcaatgccta
     1441 aaacaataac cattaataca agtgttgctt ctaaaaaact tggtgttcct ttttttccca
     1501 tgataatctc tcctttttta gttgttctta tgttctaaaa aagttaagat atcggattac
     1561 tccgccgcaa ttcatccgcg attcctcctt tataaaaata aaaagacctg ttttaaacta
     1621 aggacgaatg actccgcggt accaccttaa ttaaaaacag atctgttttt cactttcaaa
     1681 cttttattca aatgtatgga ctttccatgt aattcgtcaa ttaggcttga tcggttcaca
     1741 gcgaccaccg actttctgaa caaaacacct aaccgctact gcgtgaaaag ttgacttgta
     1801 cgaagtttac cttccataag atcatttgtc aatgaaaaag aaaatatttt ttcatttttc
     1861 tgaaaattat ttttataact ttcacacaca agattatttt ttactagaaa gtagcgccct
     1921 cttgtttttt tcttgctact atttcttgat gttcttgctt gattctttct aatagctttg
     1981 gctctttcaa cagatcaaga gctgtattgg cgagtagttc tgctgcaatc gcaatagaag
     2041 caaggccttt gtcactttta gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg
     2101 tctcagagat tgctaccgta ggctggattg ttggtatgac ttggctgaca tttcctacat
     2161 cagaagaacc gagacttgtt ctttgttccg tttctatttc ttcatctgga acacctgctt
     2221 cttttgcatg ctggaagaat aagtcatcaa aagaaggagt aacgatgaca tcatcaactg
     2281 cattttggaa caatccaaac tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga
     2341 tattttccac tttttcatat actttatcta atgtttgccg atttcctgca cgtaaataga
     2401 aacgtccagc agcgtaatct ggaatgacgt tggcagctac tcctccgtct gtaatgatcc
     2461 catggatatt gacatctttt ggcaattgca agcgtagagc gttgattcca ttaaacactt
     2521 ggatcaatgc ttccaaagca tttactcctt tttcaggtgc tgctgctgca tgggaagcga
     2581 caccgtaaaa tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt ttcccataac
     2641 gaaaagctgg atggacgcat aacgctacat cgacatcctc aaaaaaacct tctcgaacaa
     2701 agctgccttt agctgatccg ttctcgcccc cttcttcccc tggggtgcca tatacacgga
     2761 tttctccacc agtttcatca atgaattgct tcactatgct ggcggctaat acggagtaag
     2821 taccaaacaa attatgcccg catgcatgac caatacctgg taaagcatcg aattctgcca
     2881 aaaaggcaat tgtgggaccg ggtttattgg aaatataacg tgcatcaaag gctgttcggt
     2941 gtccagctac ttctttttct acccaaaacc tttctttttc taattgttta atcaacgctg
     3001 ctgaggaata aaactcatag ttacttactt caggatgttc gtggatatct agagcaagtg
     3061 cttgatattc tggtaaacga tattggatta gatccttcaa ttggtttgtt gagattgttg
     3121 ttgtcataaa aaattcctcc tatgctagtc atttttctta ttttctcccg aatgtttccc
     3181 atgcaggcac gcttgatcct tttgatgttt tctcgattac tttgactgtg tcttctgttt
     3241 gataagcgtc tactattttt tgatacgttt tattgttttc atcttctttt cttgcaacga
     3301 tgatattcac ataaggacgt gcatggtcat ccactggttc taagaagatg gcatcagaag
     3361 caggattaaa tccagcatct acagctacac cgctattgat cactgaaatg tctacatctg
     3421 acaaagcacg tgctgtttgg gaagcatcta attcagaaat gtctagattt tttctattct
     3481 cggtaatatc agaaacagtg ggcgcttgtc cttttgcctt atccactttg atcaatccag
     3541 cagtttgcaa taacagaaga gctctgccgc catttgtaac atcattaggg attgctactt
     3601 ttgctccgtc tttgatatca gctacatcct tcaaagtgtt agaataaatt cccagcggtg
     3661 cattgacagt atttccgatc gatactaaat cagttccgtg ttctttattg taattgtcta
     3721 aaaagaattg atgttggaaa gaattcaaat caatcgatcc atctgctaaa gccacatttg
     3781 gttgattgta atctgtaaat ttcacgtatt ccaaattaat gttctccttt ttcaaacggt
     3841 ctttcacgtt gtcccataca tctgtatctt ctccaataat ccctagtttt acgatagttt
     3901 cttttttgtc agttgtatct gcttttcctt ggctacaccc tgccaaccaa ataattgcta
     3961 caaatgtaat gactacaaaa cgaataattt ttttcatttt ttctcctcca ctttcaatgt
     4021 gtaatttttt taatcagttg attactgata aattgactta aaaaaactaa gattagaatc
     4081 aataatgtag ccacaatcgt tacatctgtt tggaatcgat tataccctct cgatatagca
     4141 agattcccta atccgcctgc acctactgct ccagccatgg tagtcagtcc gatcacgttg
     4201 atgatagtca acgccgagac ccgaatgata ccaggcaaac cttctaacaa atacacacgt
     4261 aaaatgatac cgattggact ggttcccatc gcttctgccg cttcgatcac cccaggatca
     4321 acttctagca gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat
     4381 ggcactaaag ctgctttcgc accaatcgtc gttccaacta agaaacgagt agtcaatgct
     4441 aaaagagaaa gcaagataat gaaagggatg gaacgaatga tattgatcag cttatcaagt
     4501 atttcaaata gcagacggtt agctagtaat ccattagggc gtgtcactac taacgtcaca
     4561 cctaagatga ttccaatcat cccgccaatg aatgctgtcc aaaaagacat atatagtgtt
     4621 tgcttcgtcg cttcgacaaa ttcatccgga atcaaataaa cattgggcaa atatgtttta
     4681 aagaaatcag tcattttatc ttcctccttc gatcactttg atacgactat ctaaatcgtt
     4741 tttttgtaaa atatttattt ttacaccaga tttgatcaaa taatccaatg cttttttccg
     4801 ctgtttggtt tctccagcta aagtaacgat caaactccct aacggcgttt cttgtaataa
     4861 ctctacattg ccatataaaa tattcgctgt tacttgatat ttgctgtaca gatgagcgat
     4921 tagaggttcg ttcgtctgat ttccaatata acttaattcc accagccatt tattggcgat
     4981 ctgatcggca aaagcagaat gggcaatgat cgtttctaat gcttggtcaa gatgagtagc
     5041 tgttcgaata aaatctttgg tcaattcttc ttcaggattg ctgaagatct ggatgctgct
     5101 acctttttca acgattcttc catcttccat aaccgctacc ttgttgcaga tttctttgac
     5161 aacttgcatc tcatgagtga tcaggacgat tgttaaacct aactggcgat tcaatttttt
     5221 taacaatgca aggatctgaa gggtcgtttt cggatcaagt gcactggttg cttcatcaca
     5281 taataatacc ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc
     5341 ccccgacagt tgtttaggat aagcagatgc tttttcttcc aaaccgacaa gttccagtag
     5401 ttcattcact ttttgacgac gttcctgttt cgattttcca gaatacttta aagaaaaatc
     5461 aacgttgtca aaaatagttc ggctttccat tagattgaaa tgctgaaaga tcataccgat
     5521 ggcctttctt tcttttcgca attctttggg agagagctga ctcagattcg tctgattgac
     5581 aatgacttct cctgctgttg gtttctgcag caaattcatc acacgaacta acgtactttt
     5641 ccctgcacct gagtaaccga cgattccata aacatcgcct ttttcaatcg ttaaatctac
     5701 agaatctact gcctgtatct ctttttcttt ttgtttgaaa catacagaaa catctcgcaa
     5761 ttcaatcatt aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca
     5821 ttttcttgag gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca
     5881 actcctcttc aagtacgatc atactctagc aagatatcgg tcgcagccgt catcgcctta
     5941 catctatgct cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt
     6001 ctcagctacc gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa
     6061 taatattcaa aaaaaaagtc cttttactaa cataaactat gtcagtaaaa ggacgacgta
     6121 tcgcggtacc accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga
     6181 gattggcgtt cgttttaacg gtcgaacagt tccgaatttg cctacttatc gacaaattat
     6241 tctccaaggc cattttcagc ttttcttact tgactcttct caccagccag agcctctctg
     6301 caaagtttcc aaacttactt tccttttcaa cgaaatcgat attttgttgt tgggattaat
     6361 ctacatgagg aaaactgaaa agtcaagtac gaaattcaaa ttattttttc tatatttatt
     6421 tttctgtttt tttatttaac atttcaataa cagttcgaag atcttttcct tccgcaagaa
     6481 gtttcaattt ttccttttct tcgacggtat aatttcggac aaaactggaa tcaggtatca
     6541 aaacaacgcc taatcctgct cgtgaagcag ctgttgctcc tactaaagaa tcttccactg
     6601 caaaggctga actagcttct aatcctgttc gttccaaagc tgtcagataa ggaatcggtg
     6661 aaggcttgtt ctccgacact tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt
     6721 taaagtgatc caagatatct gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt
     6781 tcatatgacg ttcttttaag tctgccaata tttcttttgc atatggtttt aagtggatgg
     6841 ctccgcttgt cagctgttga taaaaatatt cttcacgctt cgctcggact tcctgtactt
     6901 tttcagggct acctgctgct ttcactaaaa tatcaaaact ttgtttccag ttttttccgc
     6961 cccaagcgtc aacgatttct tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc
     7021 agccatcacg atacgttttt tctgtgtcaa ctaacaaacc atctaaatca aatattgcac
     7081 ctttcatcta tacattaatc ctttccaaaa caatacacat agtttagcac gactggtcca
     7141 aaaagaacag aagcaaagaa ccagccttct cgtttataag agttcttgga atcgacggat
     7201 atagattttc ttcacagctg tcacaagcgc cagataagcg atgactgtag caaataacca
     7261 taaccagtaa ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg
     7321 aaggacagaa ccgacaataa ttcctaatgt agtgataaca aacatggcaa atgttgcgtt
     7381 gctttggata aatggtactt tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca
     7441 tagtgattcg acaaaccatc ctgcatggaa taatgcgatg aaaatcactc gctgtgaact
     7501 atccaatgta tggaaacttc cgcccactac agcaggacaa ataacaaaat acatcaacaa
     7561 gtaagtagta atatcaaata tcgaacttgt tggaccaaag taaaccatga attccccaat
     7621 actagatgca ttccattttt ttggctcgtc caaatattct ttatccatcc gatcccatgg
     7681 caaggaaatg caggaaacat catagatcag attcaaaaac aaaagttgta aaggcagcat
     7741 tggtaaaaat ggcaggaacg tacttgcaat cagcacggaa aacatattgc caaaattaga
     7801 acttgctgta gccttgatat atttcatgat attcccaaaa gtttctcgtc cagaaagaag
     7861 gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgcag attctttcgc
     7921 aatatccaca gctgtatcta cagaaatacc gatatcagcc actttcattg caggagcgtc
     7981 attgatacca tctcctaaaa atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa
     8041 acgtgctttt tgctgaggat tcaattttac gaatacttca tggtcttcag ctacttgtgc
     8101 taattcatga tcactcattt ccgagatttt ctcaccggta accaattcat tgatctctag
     8161 acctacttga cggcacactg aacgtgtaac taattcgttg tccccagtca gaacttttac
     8221 agctacacca tgttctttca gagctttcaa cgcttgtttc gttgtttctt ttggtggatc
     8281 aaggaaagca agataaccaa tcaaaaccat gtctgactca tctttgactg aaaattctcc
     8341 aacgacgctt ggattggtct tttgtgcgac accaatcact cgtaagccat cttcattaag
     8401 tgactgtact tttgcaagta cggcttcttt tcgttctttt gttaatggtg taacagtacc
     8461 gccaagatca ataaatttag aaatgttcag catttcttca atcgcacctt ttgtgatcat
     8521 ttgtgtttta ccagctggat cagcaaccac aacactcata cgacgacgtt caaaatcaaa
     8581 aggaatttca tccacttttt gataattgat cttatctgta tctaaagttt ctgttgcttc
     8641 atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc tatttagata
     8701 cgcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat
     8761 gattttatct tgtgtcaatg tccccgtttt atccgtacat aacacatcga ttgcaccaaa
     8821 gttctggatg gcattcaaat ttttgatcac tgttcctttt ttggccatca cacttgctcc
     8881 tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac caactgctac
     8941 agatagtcca aaaagaaaag cttctagcca atcccctttt gtaagcccat tgatgacgat
     9001 aactgctgga gccatcaatg acataaattt gatcagtagc atggatgttt tatggatgcc
     9061 taattcaaag cttgattgaa ttggtttttc agatagcgtt tctgctacat gaccaaatag
     9121 tgtttcattt ccaacagaaa caatgattcc ttccgcactt ccactgatga cattacttcc
     9181 cataaagaca agattttcgt aacttgtttc gctttgctga tcgtcttcac atactttcgc
     9241 atgcttttct actggatagc tttcccctgt caatgctgct tgggaaatga ataaatcctt
     9301 tgcggacaat aaacgcatat ccgctggtat catatcccct gctgaaagcc gaacaagatc
     9361 tccacagacg atttcttcca taggaatctc tgtgtacttt ccattacgtt ttacagctgc
     9421 cgttactttc acaagtgatt tcagtttttc agccgcttga ttggaacgta cggactgaat
     9481 caacgtcatt gtcccgctga cgataaccat agtgaagata atggctacac cgaaaagatc
     9541 tttatctcca ggagctgcta atacatattc cgtgatgaat gaaataattc ctaatgcgat
     9601 caacactaat gtaaatggcg tgatgtatgc tttcaatatt tctacaatga atggtgtttt
     9661 tttgccatat gaaatcgcat tttcaccgta attttctctt gaacgttcga tttgttcttc
     9721 tgtcaatcca tttgctgatg catggaatag ttcaaataat tcttgtttcg tttttccagc
     9781 aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcaatct ctcctttgtg
     9841 tccaaaagac agctgttctc gctgcttttg gtctttagct attcatcgga tcgatagaga
     9901 aaaaactgaa aggggctgag aacagataga taatagccct ggcctttcct ctagatcctt
     9961 gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca
    10021 tgacttccaa tcatttttcg aatagttatt tttccaatta gaccacctcc tttttatttt
    10081 tcaccgatgg ggatttgcgg caacaaaaaa acctctcgcc aaatactcag cgaaaggttt
    10141 gaaaaagcat attaaaaaaa gcttctcaga tctcatcgtt gagttttgac actgtacaac
    10201 gtaaaaagca gatacctgct tttagctacc ttatgaaaag ccttatctcg acaattcctg
    10261 ttctacccat tggcgtctct cgacgttttt gggcagtagc ctatgtttgt ataggagcct
    10321 cacctaacaa atcttattcg gttttccttc gttaatataa caactaaaaa agaggatgtc
    10381 aaatcaattt tccatgtttt tcatactttt tttaaaatac acacgttata ttcctgtctc
    10441 acgcggtttt atttgtctca ttttccacct cccgctataa tagaaatata gtaggaaacg
    10501 aggtgctgtt atggaaagaa cacttgttat tatcaagcca gacggcgtga gacgccattt
    10561 agtcggaagc atcatccaac gttttgaagc aaagggactg gccattgctg aaatgaagtt
    10621 tgaaactatg actcctgaat tagcaaaaga acattatcag cacttgactg agcgcagttt
    10681 ttttgatgaa ctgatcgatt acatgacttc cggtcctgtc gtttatcttg tgctggtcgg
    10741 cgaggaagtg atcgatatcg tccgtaagat ggttggagca acaaaagctg ccgatgctgt
    10801 tccaggtacg atccgaggag actacgcgct gccggggact gaaaacatca ttcacgcttc
    10861 tgattcccgt gatgctgcgg taaaagagat cgcccgcttt ttcccgccat tcgatataaa
    10921 caaaccgtct gaaaaagcgg cgaagtgata caaattctaa actgctggtc tagttgctgg
    10981 gttcagcaat ggccagcagt tttttctcta aggacctccg ttgattcttt cctaacatat
    11041 attcgagcac tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct aaagcagcag
    11101 aaatcaatct ttgataataa tctgccatca atgtttcata gatcactgta cttctttctt
    11161 tatgtgctaa gctccgtttc atatcttcat tcacactacg tgcctttttc aaccgaccat
    11221 tttctatata aaacgaagtc aggttcatta aaaaagtaaa gagatactgc tcatgattgt
    11281 tcaactgtgc taatcgtcgc attctttttt tgattttcaa ataattgttc tcaatgaacc
    11341 gatcactgaa tatccaagga aaatcagcta gaatagatag ttcaaattga ccatagtctg
    11401 gtatctgcat caagtattct tcaatgattc ggcattcata acgtaaaaat tcaaattctg
    11461 tctcagatac ttctgttttt tcgaccatgt gaagattcaa aaccaaataa tacagatctt
    11521 ctgttttctt aaaatgccta cggttattgc tgatctcttt ataaggatca aagctgtctt
    11581 cttgtacccg atctagcatt cgtctttttt tctttaactt tattggttca tccagctggt
    11641 ttcgcatcca ttcatattct gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg
    11701 ttacaaatga catttttttc actgaatttg tctcaatccc gactagagaa gtcttaaaac
    11761 agattccttc cgctaattgg gactgtgtca acttcttgtt gactcgaagt ttcttgatca
    11821 attcaccatc atacataata gttctccttt ttaatttaaa tttttccaca ttcaataaat
    11881 agatacgtta aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa
    11941 taccaacctt caagcattag aatgttgtct aactactgaa ggttggtttg attgtttatt
    12001 gattattttt ttgctgttca acgtctgttc ttgttactgg atataagttg gttgttgttt
    12061 tattaccatt ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt tcgattgctt
    12121 cattacgttc cgcttcattt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt
    12181 ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag
    12241 tcttgacttt ttcctgatct ttggagattt cctcatcgat tcctgctgct ttcgtatcga
    12301 tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca
    12361 caaagttccc atttcgaaaa gcaactactt gatcatgctg agaagataat aagtctgtac
    12421 caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat
    12481 cgatttctcc accgtattca tgattgactg tccctttagt ataaccaggc atatggatca
    12541 tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac
    12601 caagagcgct tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa
    12661 tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca
    12721 atgattgatc cagataatgt gccgttcgaa cataattatc tactgtgcta ttgccagtat
    12781 tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg
    12841 aaagaaattt cgtgtaaaac ggctgttgta gatgctcaag atattgggca gattctctga
    12901 ataaatcttt atctaatatc ccataaccta acgtttcgtc ggattcatca aaataagatc
    12961 gatcaaagaa attgtcataa cctaagtttt tatatacatg atctctattc caaaaactag
    13021 ctacattccc atggaaaaca gcgcttgtat agccttgctt ttgtccaagg atcgctggtg
    13081 cggcttgaaa gacattgtct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa
    13141 atgtgccagt ttccaacata ttttcagcat cacttgtctt tccctgtcct acttcatgaa
    13201 agaaattgtc aaagctgatc gttgcttgat tctggaaaat actatttaaa aacggtgtga
    13261 cttcttgtcc gtctaccttc atattaatca aaaactgctg gaaactttct aaatggagaa
    13321 cgatcacatt tttcccttta gcgatcccaa atgtttcggg atttggctct gcataatgtt
    13381 tctttgtata atctaaaact tcatcaatcc cattactgct tgcatgcgcg cgaacactgc
    13441 tagtcattcc tgttttgatt ccgtcataaa tagtatacgc atcgattcct agatatttga
    13501 caatatatga acggtcaaat gttcgctgca gcaattgtgg acggttggct tcgctcagcg
    13561 ctagatttcc tgaaaaaata agccctgaaa gacaggtaac agcgatagcc atcggtttgc
    13621 cgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga
    13681 acacagcgat atccagccag tagatgacat catggggctt catcaaagaa aaggagcttc
    13741 ctgacaatcc ttgcgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg
    13801 tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga
    13861 tcaaaagaat agctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca
    13921 ataaggtagt cgcaattggg ttgatccaca agataaagta ctggatcgta ccttccacac
    13981 ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg
    14041 tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt
    14101 ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt
    14161 gttatcttaa gattttttta ttctttctaa agattattcc gtccactttt cacagcataa
    14221 aaccgctgcg atgtaagcga ttaaactaat gaagagataa aagtcttgtg gtgaccgctt
    14281 cactccacgg tattcaaaag ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca
    14341 aaattcttgt cgcagctcct tatttcttga tgcaggacag ctttttcaca acttcttcct
    14401 taaacggtgt tctcctgcca actcctcggc cacaagtcac aatatttata aaacatgaga
    14461 agctgttttt tgaaattg
//
LOCUS       DASJON010000056        14381 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJON010000056 DASJON010000000
VERSION     DASJON010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.056"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1835)
                     /locus_tag="U3989_001260"
     CDS             complement(<1..1835)
                     /locus_tag="U3989_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1714719.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="U3989_001261"
     CDS             complement(1832..2536)
                     /locus_tag="U3989_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1714720.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="U3989_001262"
     CDS             complement(2548..4857)
                     /locus_tag="U3989_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1714721.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="U3989_001263"
     CDS             complement(5052..5516)
                     /locus_tag="U3989_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714722.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="U3989_001264"
     CDS             complement(5516..6142)
                     /locus_tag="U3989_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1714723.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="U3989_001265"
     CDS             complement(6149..6514)
                     /locus_tag="U3989_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN1714724.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="U3989_001266"
     CDS             complement(6504..6842)
                     /locus_tag="U3989_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714725.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="U3989_001267"
     CDS             complement(6832..7158)
                     /locus_tag="U3989_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1714726.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="U3989_001268"
     CDS             complement(7136..7417)
                     /locus_tag="U3989_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1714727.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="U3989_001269"
     CDS             complement(7419..8783)
                     /locus_tag="U3989_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1714728.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="U3989_001270"
     CDS             complement(8796..9365)
                     /locus_tag="U3989_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN1714729.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="U3989_001271"
     CDS             complement(9337..10524)
                     /locus_tag="U3989_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1714730.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="U3989_001272"
     CDS             complement(10588..12315)
                     /locus_tag="U3989_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1714731.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="U3989_001273"
     CDS             complement(12312..12764)
                     /locus_tag="U3989_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN1714732.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="U3989_001274"
     CDS             complement(12876..13256)
                     /locus_tag="U3989_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1714733.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="U3989_001275"
     CDS             complement(13253..13639)
                     /locus_tag="U3989_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714734.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="U3989_001276"
     CDS             complement(13641..13919)
                     /locus_tag="U3989_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714735.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..>14381
                     /locus_tag="U3989_001277"
     CDS             13967..>14381
                     /locus_tag="U3989_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714736.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYR"
BASE COUNT         3997 a         3081 c         2264 g         5039 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga a
//
LOCUS       DASJON010000057        14337 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJON010000057 DASJON010000000
VERSION     DASJON010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.057"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(131..697)
                     /locus_tag="U3989_001278"
     CDS             complement(131..697)
                     /locus_tag="U3989_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1714737.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="U3989_001279"
     CDS             complement(697..1653)
                     /locus_tag="U3989_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN1714738.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="U3989_001280"
     CDS             complement(1653..2309)
                     /locus_tag="U3989_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1714739.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2679..4697
                     /locus_tag="U3989_001281"
     CDS             2679..4697
                     /locus_tag="U3989_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1714740.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4767..4973
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5124..7538
                     /locus_tag="U3989_001282"
     CDS             5124..7538
                     /locus_tag="U3989_001282"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN1714741.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7886..9439
                     /locus_tag="U3989_001283"
     CDS             7886..9439
                     /locus_tag="U3989_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1714742.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9820..11604)
                     /locus_tag="U3989_001284"
     CDS             complement(9820..11604)
                     /locus_tag="U3989_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714743.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11619..13340)
                     /locus_tag="U3989_001285"
     CDS             complement(11619..13340)
                     /locus_tag="U3989_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714744.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13440..14201
                     /locus_tag="U3989_001286"
     CDS             13440..14201
                     /locus_tag="U3989_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1714745.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4639 a         2672 c         2861 g         4165 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtaac cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2641 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2701 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2761 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2821 aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag
     2881 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
     2941 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
     3001 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
     3061 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
     3121 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
     3181 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
     3241 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
     3301 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
     3361 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
     3421 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
     3481 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
     3541 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
     3601 cgaataattc cggtaacaat accaattctt caggaaatgc tggtacatca aacggaggac
     3661 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     3721 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     3781 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     3841 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     3901 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     3961 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     4021 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     4081 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     4141 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     4201 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     4261 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     4321 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     4381 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     4441 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     4501 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     4561 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     4621 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     4681 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     4741 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     4801 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     4861 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     4921 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     4981 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     5041 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     5101 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     5161 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     5221 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     5281 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     5341 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     5401 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     5461 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     5521 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     5581 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     5641 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     5701 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     5761 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     5821 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     5881 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     5941 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     6001 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     6061 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     6121 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     6181 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     6241 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     6301 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     6361 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     6421 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     6481 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     6541 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     6601 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     6661 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     6721 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     6781 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     6841 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     6901 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     6961 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     7021 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     7081 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     7141 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     7201 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     7261 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     7321 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     7381 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     7441 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     7501 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac
     7561 tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt
     7621 tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac
     7681 acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt
     7741 ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc
     7801 aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa
     7861 tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc
     7921 agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct
     7981 tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg
     8041 ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca
     8101 cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat
     8161 ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca
     8221 acagttttta cataataaac caacttctca agatgatgac tatctttatt ttgagaagac
     8281 ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact
     8341 tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca
     8401 tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata
     8461 cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac
     8521 aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca
     8581 catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc
     8641 tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac
     8701 agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat
     8761 tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg
     8821 ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc
     8881 tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat
     8941 tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg
     9001 tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac
     9061 tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca
     9121 aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta
     9181 tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca
     9241 gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac
     9301 gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc
     9361 ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca
     9421 agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca
     9481 agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga
     9541 gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt
     9601 gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca
     9661 agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt
     9721 attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga
     9781 ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact
     9841 attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc
     9901 gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg
     9961 gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa
    10021 tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt
    10081 cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt
    10141 ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg
    10201 tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat
    10261 attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg
    10321 acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt
    10381 attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg
    10441 accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat
    10501 tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc
    10561 taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat
    10621 cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat
    10681 tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc
    10741 acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat
    10801 gaaacgaatg gccggataga tagcacagtt cataaattga gcacgtttaa aagcgttgta
    10861 ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt
    10921 gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata
    10981 ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact
    11041 gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag
    11101 accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac
    11161 ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt
    11221 gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa
    11281 tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct
    11341 taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact
    11401 gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat
    11461 tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa
    11521 tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt
    11581 gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca
    11641 aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt
    11701 tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca
    11761 acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct
    11821 gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc
    11881 aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga accgccttga
    11941 gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc
    12001 ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt
    12061 aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc
    12121 tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg
    12181 tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg
    12241 atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag
    12301 atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt
    12361 ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct
    12421 tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc
    12481 atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt
    12541 aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc
    12601 atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga
    12661 ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag
    12721 gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt
    12781 tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat
    12841 acataaaaaa caacagagat caatgtaacc catgttagta atagagaata acgaaacatc
    12901 atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca
    12961 atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta
    13021 aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt
    13081 tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt
    13141 gttcctagaa acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg
    13201 ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg
    13261 gcgatttcta aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag
    13321 aatgttttta agatagacat attattcctc cttttttcct gatacttttc gattctatac
    13381 cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa
    13441 tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga
    13501 tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc
    13561 gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag
    13621 atataccgat caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga
    13681 cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa
    13741 aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat
    13801 ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg
    13861 aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca
    13921 gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag
    13981 acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga
    14041 tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat
    14101 ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca
    14161 gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata
    14221 actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt
    14281 aatgtggggg attattagtc gtggatagat agaattttag ttttttagag gaaatat
//
LOCUS       DASJON010000058        14167 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJON010000058 DASJON010000000
VERSION     DASJON010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.058"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U3989_001287"
     CDS             <1..378
                     /locus_tag="U3989_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEN1714746.1"
                     /translation="ESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFILN
                     HGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVESNY
                     KYSKKELLELLQKLNDHIEKIMP"
     gene            complement(683..997)
                     /locus_tag="U3989_001288"
                     /pseudo
     CDS             complement(683..997)
                     /locus_tag="U3989_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(1251..2886)
                     /locus_tag="U3989_001289"
                     /pseudo
     CDS             complement(1251..2886)
                     /locus_tag="U3989_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3209..3688)
                     /gene="rlmH"
                     /locus_tag="U3989_001290"
     CDS             complement(3209..3688)
                     /gene="rlmH"
                     /locus_tag="U3989_001290"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN1714747.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4116..5405
                     /locus_tag="U3989_001291"
     CDS             4116..5405
                     /locus_tag="U3989_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN1714748.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(5444..6049)
                     /locus_tag="U3989_001292"
     CDS             complement(5444..6049)
                     /locus_tag="U3989_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN1714749.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(6254..6718)
                     /locus_tag="U3989_001293"
     CDS             complement(6254..6718)
                     /locus_tag="U3989_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1714750.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(6740..7060)
                     /locus_tag="U3989_001294"
     CDS             complement(6740..7060)
                     /locus_tag="U3989_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1714751.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(7204..8283)
                     /gene="pepA"
                     /locus_tag="U3989_001295"
     CDS             complement(7204..8283)
                     /gene="pepA"
                     /locus_tag="U3989_001295"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN1714752.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            8483..8833
                     /locus_tag="U3989_001296"
     CDS             8483..8833
                     /locus_tag="U3989_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1714753.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            9056..10003
                     /locus_tag="U3989_001297"
     CDS             9056..10003
                     /locus_tag="U3989_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1714754.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(10060..11478)
                     /gene="pepV"
                     /locus_tag="U3989_001298"
     CDS             complement(10060..11478)
                     /gene="pepV"
                     /locus_tag="U3989_001298"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN1714755.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            11627..12472
                     /locus_tag="U3989_001299"
     CDS             11627..12472
                     /locus_tag="U3989_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN1714756.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12476..12916
                     /locus_tag="U3989_001300"
     CDS             12476..12916
                     /locus_tag="U3989_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN1714757.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12966..13160
                     /locus_tag="U3989_001301"
     CDS             12966..13160
                     /locus_tag="U3989_001301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714758.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            13296..13982
                     /locus_tag="U3989_001302"
     CDS             13296..13982
                     /locus_tag="U3989_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1714759.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
BASE COUNT         4361 a         2731 c         2565 g         4510 t
ORIGIN      
        1 gaaagcaact tttttcggga tctaaatgaa attctctctt atgatccagc ccatagctat
       61 gaggaactat acactcgctt gattgactat gtttacgcaa atactttttt atatcgctta
      121 tttatcctca accatggtga tatttcgttt cagaagaaag ttaaacaagt tattgaagaa
      181 aactatctga acatatggct atatgaagac aagaaaacga ttatcacaga agaaatgaga
      241 ttctttacta cgtaccacgt ccaaggttgt ctatccgttc tcaatcggtg ggtagaaagc
      301 aattacaagt actccaaaaa agaattatta gaactcttac agaaactgaa tgatcacata
      361 gagaaaatca tgccttgaat aaaaacatac taagcttagt taagaagaac cgtaaccttc
      421 tcgtataaca actgaatctt atttagccaa attgtttact gaagattttt tgagtaaaat
      481 ttctataatt cctacttatt gaaaagtagt ttgtagtgct gcttctccct ctctaagtga
      541 tatatcttgt tgtaccaatc aaattcacct catcattaat aaattacttt taggctaatt
      601 tattacaact tataggttga agctgtaacc aatttatagt catattatat ttttatcatc
      661 cagtttattg aattcttctc gtttacctca attcattttc aaggggaatc agctcatcat
      721 ctaacatttt acgccatgct cgcttcattt cttgtgggta tttaaatggt ttcgcttctg
      781 gatgtttggc tttatatcct tcgtaatcct tactgttgat aattgctttc atattaggaa
      841 ttggatcttc acctggcata tattgcagat tagaagcatg taattttttc tcagaaacaa
      901 accactcatt ggaaaagaca tgaatcaaat catcacttac ttgtgtaaaa tagcgacgtt
      961 taatgacaaa gatatcttca ttgctatcta gtgtaccttc atttctttgt gcttaaattc
     1021 aaagataatt ttctcaataa agatttttga ttccagtaag attcctttac tccttttaca
     1081 acatagcgtt gtcctttgtt ttgaaacatc ttcttcccct ctttgccatt aaaaatagcc
     1141 caattctcta taaagaagat taagcttcat tattataata taaagtttaa atttggttta
     1201 ttacttactt tttcttattc tctagtaagc taatattgaa aaaaatgtaa tcagatcatt
     1261 atttttatat gttgtaactt cctgataaga aaactaatta aaagaaagag gtaactacat
     1321 aaactaatta aaaaagctaa cttcatagtt ggaggataaa tggaagaaac ttcaatgatt
     1381 gtttctcctt gtgttccttc aatcgacact agtcctatta tcaatctctc ttttttgtcc
     1441 atttactttt gcgactgttc cccaataatc aaatacagga agcgttattt tttgtccttt
     1501 agattcatgt actgtataga ctattttcga atccatcact tgataaggca tagtatgcca
     1561 ttccttgcca attcttactt catgattctg caaatgttct ttgtattgta atgcttccgt
     1621 gggtgcatag tcataaagtc ctccgggaag gagtgtcgtg taacgaataa gattttcttg
     1681 attcattacc aagtgatctc tctgagtgac aactttatgt tcatagtttg cttttgttgc
     1741 agcagataca gccaacagta ataaaacaat tgccgttata ttaactactt ttctttgctt
     1801 agctgatgtg ctcttcatga aaacgaacga taaaatcaac gagccaaaca gtacttgaaa
     1861 gcctaagatt cgccaaggaa attgaatcat ttgaatgggt gtattttgta ataaagacca
     1921 tggaaaaaat ttggtggata agagccaaga gactaccgca ctaaggtaaa tcactttttc
     1981 tttgcgatta aatcctttaa aaaagaagag tggacagatc aaagaaataa taccaatgat
     2041 tcctaccgta aaggttgtca ggtcattatt catcgcacca atcaaactat ccgagacatt
     2101 taatgcttgg tgttgtaagg tcgttttatc tggctcatta atcgattgaa ccatgatttg
     2161 tcggaacatc ggataccaaa agtatgctgt taaaagaatc gtcattccta ctgcctttga
     2221 caactccaca atcaaccgga tgctttcttt ttttcttgtt tcacttacta caaagccgcc
     2281 taagactagt aagccaacgg ctattacact cataaaagtg gaaagaacat gtgtataaat
     2341 gagtaagctc atactgactg ctaatggaat tgcacatggc tttttctcat aaatcagtgc
     2401 gtaagcataa tacataacta tcggtataac cgtaagtgca atcgcttcag caatagcaga
     2461 acgatgataa atatcaatcg tacggtaccc ataaaatgta tataaacagc taaatataaa
     2521 agccgcatcg attcttttta aaaattttag tccataatga aacatcagca tgatagtaca
     2581 aacattcaat agccaaacat ataagatata actaacgatt aaattattac ttatccaata
     2641 aaaaatgact gccggaaaaa aggtcaagta gggataaaaa taattcactc cgctcccata
     2701 atgattaaac gtcgtaaaat tgattggacc agaaaaaatt gaagataacc ctttgactct
     2761 agcaaaatgg aaggcaatat cccccgatgt agaaatagta tgaacatttc taaaataatt
     2821 gattgaataa agtccagcaa taattgctag gagaatggac aatattattt ttttctttac
     2881 ttgcattact taattatata ctcctttcat ggattgcttt atctagtata acttaaattt
     2941 taaataaatc gttatttatt tttactctca cttaaaaaag cactaaaatt ataagagagg
     3001 gaggtaatgg tctgaacacc aagaattgtt ccaacatcga attgcttaac taatgagttc
     3061 aaagcttaat tatgtggctc ttatatttac cgagcgtgga ttgcgatctc tttagcgaaa
     3121 caaacatctt tcataaaaaa aacagccata tccgctttct tggatatgac tatttactac
     3181 tactttacta tctttcattc ttcttgtctc acttatgata aggcgccccc gcattgatcc
     3241 ggaaggcacg gtagatttgc tcgacaagga tcagacgcat caattgatgt ggatacgtca
     3301 tttttccaaa agagatctga gcattgcttc ttttcatgac ggcttcgctg agtcctaaag
     3361 agccgccgat aacaaaaaca aggtggcttt ttccttggat acctaattgg tcgatttgtt
     3421 tagcaaaaga ttcacttgta ggatttttcc cttcaattgc taaggcatag acgtattctt
     3481 gttctttgat tttcgctaag atccgttccc cttctttttc ttttacttgt ctcatttgcg
     3541 cttcgcttaa attctccggg gctttttcgt caggaacttc gatcaattcg atttttgcat
     3601 aggcattcag ccgtttgaca tactcattga taccttggat caggtatttt tcttttaatt
     3661 ttcctactga gataattttg atattcatgt tttctctcct ttggtcttca tgctcacttt
     3721 gtttattata atcaattttt tcgtctatat gtttattttg gctatttttg ttaaaaagat
     3781 ttccccaaca gttatgcaca acatccacag gtttatccac agaaaacagt gttattttac
     3841 cttatttcat cacattcatt aggcaaacat acgttttata agaatttatc cactttatac
     3901 acaggctgtg gatatgtttt atcccttatt tttccaatcc tatccgcgta gttttacaca
     3961 gtatctgtgg aaaaagaaaa tcaaatattt tcatctgaaa acaatgattt ttcctaaagt
     4021 ttttcataaa aagttcacag tctcaaagta gactaaagtc aacaaataag actatactaa
     4081 cagtagataa atgaatgtaa ttggaggagg aacacatgga tagaaaaaat gtgacaccaa
     4141 agatgaaaaa aagtaaaaat aatagcctct ggcgtaagtt aggtctgggg cttgttggag
     4201 ggatcgttgg aggactactg acagctggca ttttttatgc agcgatgggc actggcaaca
     4261 atagttctac tgctacaagc ggcaatcaga attctgccgg tgaaacagtt gtggaaaatg
     4321 tcaaagtcaa tgtagattct gatatcacca aagcggtaga taaagttcaa gacgctgtcg
     4381 tttctgttat caacttacaa agtcaaaacc aatcaagcgg ttttggcgga ctattcgggc
     4441 agcaagaaga aagtagcagc tcagatgata gtaatttgga agcttatagt gaaggaagcg
     4501 gcgtgatcta caaaaaagac gggaatactg cttatgtagt cacgaataat cacgtagtag
     4561 atggccagca aggtcttgaa gtcttgatga aagacggtac gaaggtaaaa gctgaattag
     4621 ttggaacaga tgcttattca gatttagcgg tgttgaagat caattcagac aaagtcgaaa
     4681 cagttgcctc ttttggtgac tcaagtgctc tgaaggttgg tgagcctgcg attgcgattg
     4741 gttccccact aggatctgaa tatgcaaatt ctgtcacttc aggaatcatt tcttccttga
     4801 accgacaagt gacaagtacg aatgaatcga acgaaacagt caatatcaat gcgatccaaa
     4861 cagatgcagc aatcaaccct ggaaattctg gtggtccttt agttaatatc gaaggacaag
     4921 ttatcggtat caactcaagt aagatcgcaa gtacttctga atctacttct aatgtaagcg
     4981 tagaaggaat gggatttgct attccaagta acgatgtagt gaatatcatc aatcaactgg
     5041 aaaaagacgg aaaagtgaca cgtccagctc taggtatcac aatggtcgac ttatctgccg
     5101 tttctactca gcaacaagaa caaatcttga agattccttc atctgtaact aacggtgtca
     5161 tcgtgacaaa tgtccaaaca gctacaccgg ctgaaaaagc tggattgaaa caatacgacg
     5221 tcattactaa gatcgatgac aaagaagttt cttctggtgt agaacttcaa tctgctttgt
     5281 ataagaaaaa agtgggagat tctgtcaaaa tcacttacta tgaaggctcg aaagaaaaaa
     5341 caactactgt tgaattgacg atcgatcaat cggcattaaa acaaaatcaa aatagtgaaa
     5401 attaataaaa aagaggttgt gacaaagact tgtcacaacc tctttatttt ggaaatgctt
     5461 ctccgactac ggcatcctca ggcaatacta agattccttt ttttgctgga gcatttggca
     5521 aatgcaattc acgagcggaa cagatcattc catagctatc tacccctcgc aaagttcctg
     5581 gccagatcat cattccatct ggcatcatag ctcctggttt tgcaacgaca actttttgtc
     5641 cagcttcgat attgggtgca ccgcaaacga tttgcaatac tgtaccattg tctacttctg
     5701 tctcagtaat cgataaatgg tcagaatcag gatgtgggac gcatgttttt acgtacccga
     5761 caacgatctt cggttcatga tcagcttcaa gctgttcttc gaatcctgct tctttgatca
     5821 atcgattcaa tttcgctaat tcctcatctg ttaagcttac ttggccgttc ccttcgattg
     5881 ttagatgatc ggagatatgg aaaaagttcc aagcaacaac ttgaccttgt tcatctttga
     5941 tccgagcaac attccatttg cgttcaacag tgttttctgt tcctttatcg tcggctataa
     6001 taaccatcaa ggtgtctccg acgtgtgctt tgttataagc aaaaatcatg ttctttcctc
     6061 cattatcctt tgctgagatt tatcttaaca aaaaattgca ttcactgcat gaaatttttt
     6121 tcatgcttta tttccgaaga agcctcttct ggaacaccat ttattcgtaa caagggactg
     6181 tgacaagact tttgtcacag tccctttact gttttagtta attgattaaa aaggtcgtac
     6241 aagttccact tgcttaatta cttggctgtt cttcttcgtc agaaacgatc aagacatcac
     6301 aaggtgaatg acgtatcacg tagctggcta cacttcctgt cataaagcgt tcgactgcat
     6361 ttagacctga ctgcccaacc atgattagat cgatctcata tttttttggc aatttgactg
     6421 ccatggattc acgaacagaa ccatatgcaa taattccttc tatatttgtg taatcaacag
     6481 atttcccata ttctttacat tcttcgatca tttccttggc tgtttcagtt tgctgatcga
     6541 ctagattttg atcaataggt gcatagccca taaagttata aaattgctga tcgattacgt
     6601 ttgctacata tactttccca ttattacggc gagccacttc taccgctttt ttgaaagcag
     6661 caagtgcttg ttcactaccg tcgataccta ctaaaatatt cttatattct tgattcatct
     6721 tgacccctcc taacagatgt cagccttttg ttacactttc taggaacgat tcgatttcct
     6781 gttttgtttt acgatcttta ttcactagac gacctaattc ttttccgtct tggatcacga
     6841 caaaacttgg gataccaaaa atgttccatt cagcggctac atctatgtac ttgtcacggt
     6901 ctacttcaat aaattgccaa tcagaaaagt cattttcaat ttcaggcatt tgcggtttga
     6961 taaaacggca gtctccgcac cagccggcag taaagaaaaa tacactcttt ccagtttcta
     7021 cgtagcctgc taattcttct aaactatttg ggataatcat tctttgtcct ctactttctg
     7081 tttatgtatt tttcaattaa attatacacc tttctttcga actaacaaac attgatcctc
     7141 tttttataaa aaaaatctgg caaaaagcaa tcttttgcca gattttatag tttcactatc
     7201 gcattatttt ccgtatacga tcgtatttac tgttgattta tccaaacctt tcagtgtttg
     7261 aatcaacatt tctcgtgcag cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg
     7321 gatataacgt cccactacgc caatcactgt acttgggatt ccttcgtttt gtgtatgagc
     7381 agcacctgca tctgtaccgc cttttgaaac aaagtattga taagggatat tgtttgtttc
     7441 agcgatatcc agcaagtact cgcgcaaacg aggaagcatg atcaaacctg gatcttggat
     7501 acgcatcaat gtaccttctc ctaaatgtcc gaatgtgcca tttttcgtca ctgtatcatc
     7561 tgctgcagag cagtctactg caaagaacag atctggcttg aatttattga cagaagcttt
     7621 agaaccacgc aagcctactt cttcctggac attcgcgcct gcaatcaatg tatgacctaa
     7681 tgtttcattt tgcaacgctt ctaaagcttc taataccatt gtacagccat agcggttgtc
     7741 ccatgattta ctgataatat tctttccgtt ggctgttttg atcgtttcag cgtaaggaac
     7801 gattgtgtct cctggaagga caccgtaact cattgcctct tctcttgatt caaacccagc
     7861 atcgaacaaa atatcggtta cttctactga tttttgtccg cttgttcctc gcaataaatg
     7921 tgggggaacg gaagaagaaa tacatggata attcttacca gtagaggttt taagtgtaaa
     7981 acgctgagca gaaacaacgt aaggattcca gccgcctaga ggtacgactt taaaaagccc
     8041 gttttcttgg atttgtgtca gcatgaatcc gacttcatcc atatgtgctg cgaccattac
     8101 acggggtgca tccgcttctt catgatggcg cagaccaaaa atgccgccca aaccatctaa
     8161 ctgtaactca tctaccagcg gttccatatg actttccata taagcgcgaa catcttgttc
     8221 aaaaccgctt gttccttgta attctgttaa ctctttgatt cgttgaaatg ttttttcttc
     8281 catatgctat tcgtctaaat ttcagactaa actcctctca gacttatatt gatctgcgga
     8341 taaaatatga ttttttctgc agaccgatcg tatttattat agcttatttt ttctggtagc
     8401 ggttatttat ttcaaagaaa atatgagagt gtggtaaaat aagcacaagg agtaacaagt
     8461 gagaagaagg aggtatgtcc acatgtatga agaaagcgaa ctaagctatt ttaaaggcgg
     8521 gttagcagtt ggaatcggtc taggattatt gagcggggta gcttcaacga tttggtatca
     8581 tcgcaaaaaa gtattgtcag cagaccaagt acttgaaaat atcaagtcat cttttctaaa
     8641 agaaggtgca atcgaaggtt cttggatctc ttttgaaaag gaacctatta ggaaattcgc
     8701 cgttcggtcg agtgcttatc ggggaggtct ttctcgaata gaagatggcg aaactgttta
     8761 ttatgaattt ttagcagatg ctcatacagg aaccgtatta gaaatttcaa gaaaaaaagg
     8821 tgcaacagat taatacgtca aaaaagtgag agaacgaata cgcacccact attcaaaaga
     8881 gaggtatcat gtccttcttt tgtagtgggt ggttttttag atagagcttt ccaaacatat
     8941 attccagtat tttcttttta aataaatgat atttagcggg aaaaactagc atttcctttg
     9001 attatgaggt atttttaatt tagcttttgt tttctatagt taaggagagt gaaaaatgga
     9061 acaacaagaa aaaattgctg ttctgattcc ttgttacaat gaggaagcaa caatttcaac
     9121 agtcattgca gactttaaac gagaattacc agaagcagat atttatgtct atgataataa
     9181 ttcgaccgat cgaacatacg aattggccgt tcaaggtgga gcaatcgtaa aaaaagaacc
     9241 tcgtcaagga aaaggaaatg tgatccgcca aatgttcttt gatatcgatg ccgactatta
     9301 tttaatggta gatggcgatg atacttatcc tgcagaagct gtacatggtt tgctcgagaa
     9361 attacgttct ggtgaagcag atatggtcat tggtgatcgc ctatcaaatg gaacctactt
     9421 tgatgagaat aagagacctt tccacgattt tggaaataac cttgttagaa atacaattac
     9481 tagaatgtat aaaacaaaaa ttcttgatgt catgaccggc tatcgaggat tcaatcgtat
     9541 ctttgtcaaa agttttccta ttatgagttc tggttttcaa atcgaaacgg aattgacgat
     9601 ccatgcttta gataaaaaat tcaagttcgt tgaaatccca atcgattatc gtgatcgtcc
     9661 tgaaggcagc gaatcaaaat tgaatacatt ctctgacgga ttcaaagtga tcatgatgat
     9721 tattaaaatg tttaaagatt ataagccttt gcttttcttt gggatttgga ccatcttctt
     9781 ctttttattt ggattggtca cggggattcc tgtgattcgc gaatatatgc tgacaagctt
     9841 tattacacgc atcccttctg cgattttaag tactggattg atgactttag cacttctttc
     9901 attagtaaca gggttgattt tggatacagt cgtcacgaac gctaagaaag aatatgaatt
     9961 gaatctatat catgtttatc atgaatacca cagtagaaag taaaaaaagg atcaagactg
    10021 caaaaataat ttgcaattct tgatcctctt tttaattaat tatttcgcca aacgatagat
    10081 ggcatctgca tagattgctg ttgctctgaa caagtcatca agactcataa attcgtttgc
    10141 ttgatgcatt gtatcagtat atcctgggaa catcgctcca taagcaacgc cacgtttcaa
    10201 caaacgtccg taagttccgc caccgatgat ttgttcgtag cctttttctc cagtgtggtc
    10261 ttcatagact tgcaataatg tagccaccaa tggatcatcc attggtacgt aatgtggttc
    10321 cattacgcga gcacctcgag taactgttgc gccttcttgc cccactgttt gttcgatgtc
    10381 actttgcaat ccgtctactg ttacaccttt tggaaaacgg aaattcatat tgatgaagtt
    10441 cgcttcctct gtaccatttt cttcaaaagc aaatagtcca gcattcattg tcaatttgcc
    10501 cattttttca tcttcataag cgactcctag tttttctcca taaaaatctt catggacata
    10561 agcagctgct acatggataa attgtttagc agatcctagg aattcatagt tatctaagaa
    10621 caatgccaag aaagaacctg cattgatacc agattgtggg cttgcaccat gtgcgccttt
    10681 accaactaat tcgattttta catacgtatt gtccgcttca atcgttccgc ttactggatt
    10741 tgcttcgatg aattggtaaa atgcttcttc catcgcgata gctgcatctg cacttggtac
    10801 ttgaagtgct gcagttgctg ttcctggcac catattttcg cgaagtccgg aaacaaaaga
    10861 tttcaagaca taatctcctg cattgtcgcc tttgaagcgt aaagctaaag aaacatttcc
    10921 tttttcacca ttgatgattg ggaattcagc atctggtgag aaaccaaaat ctggttcttc
    10981 ttcatgttcg aaataataag ccatatcgcc ccagccgctt tcttcatcgc tgccgactac
    11041 gaagcgaact ttttttgata gtttcaagtc aagttcttta atgatcttca tcgcatagta
    11101 agctgccata cttggacctt tgtcatcgct tgatccgcgt gcaaagatct ttccatcctt
    11161 gattactggt tcataaggat ctgtgtccca gccatctcct gcaggaacga catccatgtg
    11221 tccaaatatc cctagcgttt cgtctccttc gccaagatca atatgccctg catagttatc
    11281 gacatttttt actttaaatc catcacgttc gccataagct aacatatgtt tcaatgcgtc
    11341 gcgaggtcca ggtccaaatg gtgcatctgg tgttactttt gagtcatcgc gctcgctgtt
    11401 gacacgtaaa agatttttca agtcttcaag caagtcttct ttacgtgctt ctacttcttt
    11461 ttgccaatca attgtcataa agttaatcct ccacttattt aagtcttcgt gttcatgata
    11521 ccacaaatta cagaaaaaaa tagttcaaaa aatctgacaa ttccttttct tatctgtttt
    11581 tctctaaaaa aaatatacgc tataatgagt aaaaggaggt ttttagatgc tgataaacga
    11641 acaactagtg aaaagagtca tcacacctcg tccaaaaatt tcacataaag gaacatttgg
    11701 acgtatcgtt ttgattggcg gcaatgaaca atatggcgga gcaatcatta tgagtgcttt
    11761 agctgccgta cacagcggag cgggtctcac gactgtagta actgatgaaa aaaatcggag
    11821 tgctcttcac agtcatctgc cagaagcaat gattgtagtt tggacggata ctgaccagtt
    11881 tctttccgta ataaagcaag cagatgtact cttgattggt cccggattgg gtacttcttc
    11941 taagagtttg gaacgtttca aacaagtact agaaaaccaa tcaaaagaac aatggctggt
    12001 gattgatggc tctgctttaa gtttacttgc tgctaatcat cttttgctac cctatcccga
    12061 acaaactgta cttacacctc atcaaatgga gtggcaacgg ttgagttctc taccgataca
    12121 agaacaacgg gataaagaaa acaaaaagaa gcaacaggaa atcggtgctg tgctcgtttt
    12181 gaaaagtcat gagacaaaaa tttatggtca ccagcacttc tacaccaatc ctttaggttc
    12241 tgctgctatg gcaacgggcg gcatgggtga cactttagct gggatgatca caggattttt
    12301 agcccagttt cctcaaaatg accaaacgat tgcagcagct atttatcttc atagtttcat
    12361 tggagaccag ctggcaaaag aacaatatat tgttttgccg acacaaatca gcgaagcact
    12421 tccgaaatgg atgaattatt tttgtaaaaa atcaatggaa aacgaggcat gagtcatgga
    12481 gaaatttaca ggttatgtgc gagaaccttt ttattatgaa acagatcaaa tggggattat
    12541 tcatcacagc aattatatcc gctggtttga agaggctcgg gtagccttat tggtacatct
    12601 agggtttccc tataaaaaaa tcgaagaatc tgggatcatc atccccgttc tggaagtaaa
    12661 ttgcagctat aaagaaatga tccattacgg agaaaaagtg agcatccatc caactatcca
    12721 aaagtatacg gggacacggt tggattttag ctacgaaatt gtgggaactg aagacaaaaa
    12781 attaaaaact actggcagta gtaaacactg ttttttactt tctgattcgc aacgattagt
    12841 gaagctgagc aaagtcaatc cggagttaga tcagttattc caagcgtacg cttctttgga
    12901 tgatgtaaat tcttaataaa gaccgtctaa gcatttttct tgtattgtgt cgagtagttc
    12961 gtataatgaa aagaaaaaag caggtgatcc ccatgtatgt cgttaaagta atgcacggtt
    13021 acatcgataa aactggctgt cgcactcggg aaaaaaactt agacaatctg ctcattttca
    13081 aagacaaaaa agaatccgaa gcttttgcta aaagaatcgg cggtcgtgtc aaaccgatcc
    13141 aagaggtacg accagattga ttcaatattt ttcttttcga ggctctaata ggcatgccta
    13201 tcagggcttt ttttgcagac tgtgttttta tgcccacacg atttgtaagc gatttatgtt
    13261 ataatagatt atatctgcat agaaaggaag gttaaatggt tatcaataat ttgcaacagc
    13321 aagcttatga atctatacgt aaacaaatca tttattccga gttagcacct ggaagtaaaa
    13381 tctccgataa agtgttagaa gaacaactag ctatcgggcg tacaccgatt caagaagcac
    13441 tgatccaact gcgcaatcaa gagcttgtaa aaaccattcc acagtccggc acttatgttt
    13501 caaaaatcga tatccgatct gcttcattag cccgttatac gcgggaaaag ttggaaaaac
    13561 cgatcctgca agaatgcagt gcgaaaatga ccaagcaaga tcaagctaaa ctagaaaaaa
    13621 tactagaaca aaccgatcag gcagtaatcg ctcaaaataa aaaagctttt tttgatttgg
    13681 atactgattt tcaccgaact tgttatgaaa ttgctggaaa acgagaaatt tgggattggc
    13741 tagaaagtta tagtacgcac ttgaaccgtt tcagatggtt acgtttgacg atttcagaat
    13801 tagactgggg tcgagtatta gatgaacatc agacaatgct tcaatcaatg attgatcata
    13861 actttgatga agtcggattt ctttgcacga tgcatttaca tatgattatc gaggagcaag
    13921 aatacgtgat tcataattac cccgattatt ttgaagatat tcaggacaga cccatcaaat
    13981 aagaggtttt ggaatcagcg ttttgacctg agtattagag aaagaaacgg caaaatagtt
    14041 cctcatattt tgccgttttt tttgagctaa taccgagggt cagctgattg cacaccgttt
    14101 attgagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaata
    14161 gcttctt
//
LOCUS       DASJON010000059        14050 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJON010000059 DASJON010000000
VERSION     DASJON010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.059"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..684)
                     /locus_tag="U3989_001303"
     CDS             complement(37..684)
                     /locus_tag="U3989_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1714760.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="U3989_001304"
     CDS             complement(692..1378)
                     /locus_tag="U3989_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714761.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="U3989_001305"
     CDS             complement(1384..2457)
                     /locus_tag="U3989_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714762.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="U3989_001306"
     CDS             2679..3353
                     /locus_tag="U3989_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1714763.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="U3989_001307"
     CDS             3358..4698
                     /locus_tag="U3989_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1714764.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="U3989_001308"
     CDS             complement(4847..5833)
                     /locus_tag="U3989_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN1714765.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            6557..7588
                     /locus_tag="U3989_001309"
     CDS             6557..7588
                     /locus_tag="U3989_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1714766.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            7697..9229
                     /locus_tag="U3989_001310"
     CDS             7697..9229
                     /locus_tag="U3989_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN1714767.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            9368..10180
                     /locus_tag="U3989_001311"
     CDS             9368..10180
                     /locus_tag="U3989_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN1714768.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            10170..10655
                     /locus_tag="U3989_001312"
     CDS             10170..10655
                     /locus_tag="U3989_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1714769.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            10678..11223
                     /locus_tag="U3989_001313"
     CDS             10678..11223
                     /locus_tag="U3989_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN1714770.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(11296..12939)
                     /locus_tag="U3989_001314"
     CDS             complement(11296..12939)
                     /locus_tag="U3989_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1714771.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(13068..>14050)
                     /locus_tag="U3989_001315"
     CDS             complement(13068..>14050)
                     /locus_tag="U3989_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HEN1714772.1"
                     /translation="PPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDMEAHDKEIKKQ
                     LNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLSRQILELLDNE
                     QTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDESGSMIRDDRI
                     EAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGFQWLNEKLVTM
                     KPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKAKEDIQSVLKE
                     YERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIARYL"
BASE COUNT         4230 a         2648 c         2553 g         4619 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
     5881 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aatttgatta
     5941 aaacaattta attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat
     6001 gtcatttgtt tctatatgaa tttttatgaa aaatttcata taatttccac tcgttgtgat
     6061 ggtttaattt atccaatcca tcaataagca aacaacttat ataaaaaagg attaattctc
     6121 aatataattg ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt accaactatt
     6181 tctaatactt ctttaagaat tatcccaaca atatcgttag acaaaccatt attgaataaa
     6241 tggagagcca atcgattctg aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc
     6301 ctcctgccat tagaacgaac caaatccatt tcattgctca tgaagtaacc acatagctaa
     6361 aaagaggata aatcaattaa aagatcaaaa gaatgactgc actaataaaa taactaaaaa
     6421 tgtatcttct tacttcatat cttttagcta gtacttagtt aaaaatttgc ttcctaagat
     6481 caagacaaac tgtctatctt catttgatga aatgcggtaa aatatagaaa atgaacacta
     6541 ttggagggaa tttgtaatgg acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca
     6601 acgtacacac ttgctccctt ccgtctttca acagttttta gaagaaaatc gttcttccct
     6661 ttcattaggc agtcgctatg aatacgcaaa agattttcat ctgtttactg attatttgag
     6721 aaaagtattt tctgaagaag tggatgatca gctgattatc catttagaaa aacaagatta
     6781 tcagaatttc ttagctaaaa tctatcgaca tactcgttcg ttccaaacta cagcgaacca
     6841 agaaacggag caattgtttg aaaatagcta ttacgggatt tcaaggaagc aatattcatt
     6901 aaatcatttt ttacgatttt tgtatcaaaa aggactgatt gaagaagaga tttctctttt
     6961 gggtacaagt ttaccggaaa caaccaaagc agctccgcga tatatcgatg cagaactttt
     7021 caaagatttt gagcatttgt ttatcccgat tgaaggcttt caaacaagca gagaagctag
     7081 ctttgaagaa aagaatagac cgaggaacct tgcaattact gccattcttc tgtattgcgg
     7141 attaaagatc catgaagtag tagaacttaa acgtaaagat gtatctttga ccaagcagat
     7201 tcttaatctg aatagaaaag aaaacagact gaataaagta ccaataccaa aagaagcgat
     7261 gccatttctg gagaactatt ttaagttaac agcacaaaat acggacgaaa attcttttgt
     7321 ttttcgttcc tcacatgatc agcaaatttc tcctcggact attcgccaaa ttttgagcaa
     7381 aattacagta tataaaaacg tatcgccaga actatgcaga aaaacatatc gttattatgc
     7441 agaactatat ttagatgatg ctgacgcggt aaattattta tgtggtaatc gatacctctc
     7501 atcccattct tttcaagata tctgggaaaa gatggttgat ttttcttatc acgaattagc
     7561 ggcattcgtt cgggtttctt ccatataaaa caaatgtatg atcttttttt cacaataaac
     7621 tatgttatta tataaaggat tatgttacga ttatcttgaa tttgtatatt acttaacaaa
     7681 agataatgga gtgtgaatga tggagaccat aacgtattcc cctttgaact tatttacgaa
     7741 ttttcaagaa gctgcagcta acttttccga gaccccgatt atctttgatg agccactttc
     7801 agctttccca gaattaggac ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa
     7861 acgggcttat caattggctc atcttggagt gaaatccgga gacaaagtta ttatttataa
     7921 gagttccgct tttgacacat accttctagc tgtttctgtg tcttaccttg gtgcagtgcc
     7981 tgtcatgact tcttatcatc taccaacaac aacaatggaa gtatttatcg atcgtttaga
     8041 agaccctttc attttatttg atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg
     8101 aacaaaatca aaacagattc ctattcttta tttaatggaa caacctgctt tgtctgtttc
     8161 tcaatcattt ttagacaaaa atgaaatttc ttatatgacc catacttctg gtacaactgg
     8221 tatccctaaa cttatttgtc attctgctca ttctatggga tggcggacga aatggcaaaa
     8281 aacagttttc acaaaaattt cggaaaagaa attaatcgct tttcacatct ctccagtgca
     8341 ttccagattc aatattggta tctcttcttt gatgtccatg ggatttccta tgatgccttt
     8401 agcaaacgca caaagttcaa aagttgttca tatgttggaa gctcaccggc caattgcact
     8461 agagacccat ccgaataact ttgttcagtg gcgttttact gctaaggagc atccagaagc
     8521 tttttcgggt atccgatact atcactccac ttttgatgct ataaacaacc agacaatgaa
     8581 gacatttttg cgaacgtcga tggcacagga tgcggtattt cttcaagtat atggccaaag
     8641 tgaatgcgga ccaatgattc ttaaggctca tacactagag agtttgaaaa caagtgatgc
     8701 aagagatatg ggcgttggac tggaagacat gacaagcgct cggattacag attcagttgg
     8761 gaatgtccta cctgaaaaca cagatggtca tatacaatta ctgtctaaag gacgggcttt
     8821 gacttattac aaggaagatg cacggttcca ggaaaatgtt tatggcgatt ggtgggatag
     8881 cggagattac ggttttatgg ataagcatgg ccacctattt ttgaaagatc ggcaagtgga
     8941 tttgatcgaa acgatcaata gtacattagc tattgaagac tttttattag attcgttaga
     9001 ttttttggca gaagtagtga ttgtaaggga taaaaatcat tctccacaac cgattattgc
     9061 tttagctcct gataaagaaa tggattggaa tcgatggtgg gagcaagtcc atgaacttcc
     9121 tcgcttgaac gtccctatca tccgtgattt tgatgcaatc ccgcgaacag ctacaatgaa
     9181 agtgcaacgg cttcaaatcg agaaagaatt aaaatcacaa aagtcctaac tatttaagga
     9241 aagaaagatc cattttggtc tttcttttct ttttgttttt ttaaattgga tgagataaaa
     9301 atatgccaat tggctgtttt tcttttttga aaatttcgtt atactcttat tcatggagga
     9361 aaatgttatg aaaagaatct tgacaattgg cggttctgat ccatttgctg gtggaggaat
     9421 ccaaacagat ttaaaaactt ttgagaattt tggtctattc ggactaagcg ccttgacttc
     9481 tataggggca ctagatatta atgagcgatt tatgctggaa tctatatctt tggaattgct
     9541 taaacgacag ttagtttcga ttgataaaat gacttcatta gatggtatca aaattgggtt
     9601 attgaattca agtgaagcaa tcatggttgt tcgagacttc ataaaaacaa aacaaaatat
     9661 cccaatcatt ttagaccctg ttttggcatt caaagaaact gaagctgtga tcaatcaatt
     9721 gtatatttcg cagctcatcg aagaactttt tccacttgtt acgatgatta cgcctaattt
     9781 ggcagaagct gctttacttt ctggtcaagc tcttccctct tcgttaacag aaatgatcca
     9841 tctcgctaaa gaactcactg cttcaggaac aaaaaacatt gtgatcaaag gcggcacaag
     9901 gatggctgga gatgaagctg tggatcttct ctattcaatt gatcattatg aggtattttc
     9961 tcataaaaaa atcgacacta caacagtaaa cggggccggt tgtgcctttt cttctgcaat
    10021 aacagcgaat cttgtgttag gggcagattt taccaaagca gtagcagaaa gtaaaaaatt
    10081 tgttgcccat tgtatttcta atggcgtgct gatgaacgat cataccggaa gtgtctggtc
    10141 tggcggtatt caaaaaggag gaatgagaaa tgaaaactaa gcaattaacg atctatgcta
    10201 tgctgattgc gttgacagtt gccctttcat taacaatttt gatccctgta ccggctacga
    10261 atggatttgt gacattatgc gaggccggga tctataccgc tgcttttctt tatggtccaa
    10321 cgggtggatt agttgttggt gcttccagtg gtctattgat cgatttgatt tcaggatatc
    10381 cgcagtgggc agttttttca ttagtgatcc acgggttaca aggatatgtt gctggttctt
    10441 tcagcaaatc ttctactcgt atgtggggat tagggctttt attaggaagc ttagttatga
    10501 tcatcggcta tggtatcgcc ggttggattt tatatgattg gccggctgga gtggcttcta
    10561 taccaggaaa catcttccaa aatctgattg gaatgggact tgcctttcct ataactgcaa
    10621 gtcttcaacg tattcaagca cggaagcaat attaattgaa aaagaaggtt ttagaaaatg
    10681 aagttgtcag aaaaagaatt aaaagaccaa ttgactgaaa tagttaatga catattggct
    10741 gaggcacact tgaaaaaagg tgatcttttt gttttaggat gcagcacaag cgaagtcgtt
    10801 ggaggacata ttggaaaaaa ttccagtgcg gaagttggtc aatggatcat ccgtacgtta
    10861 aaagagttgt tggatcctaa agaaattgcg ctagctgtcc aaggttgtga acatttgaac
    10921 cgagcattag tagtagaacg aacagtggca gaagcaaaaa atttcgaaat tgtctctgtt
    10981 gttcctgcac tacatgctgg aggtgcttgt tctgttgctg cttttgacca gttcaatgat
    11041 cctgtcgaag tggaacacgt agttggacaa gccggtatcg atatcggtga cacatcaatc
    11101 ggtatgcata tcaagcatgt ccaagtacct gtcagacctc gtttgaaaac ccttggacaa
    11161 gctcacgtga cagcccttcg ctctcgtcct aaatatatcg gtggtcctag agcgaattat
    11221 tgaaatgagt aattgacaga gcaatctgaa aaacttcctc gcaaagtcca actaatgaac
    11281 tttaacgagg aagttttact tgtttgtttt atataaccga gcttcataag gtgctagtat
    11341 gcctttagct tcaaattctc cgctagagga atttgacaag agtaatgtcc aggactgatc
    11401 tgcttctttt ggaagttcat agtcagcaag tgattctgtt aagtttacca taataagata
    11461 ttgttcatct tctaaatatc tttcatagac ataaagttgt ggatgttcat gaagatattc
    11521 tttataagaa ccgtatatca gacctttatt ggtttgtcta atgcggatca tgttcttata
    11581 gaattgcagg accgactctg gttcatattc ttgttgttct acattgatcg ttttggtatt
    11641 tggattagtg atcagccaag gttcatgtgt agaaaaacca gcatattgtt ccgaagtcca
    11701 ttgcatgggt gtcctgctgt tgtctctcgt tgtttcacga atgatatcca atgcttcttc
    11761 tcttgtctta ccttccgcaa gcagcctttt ataaagtctt ttcgaatcta ctgcatcaac
    11821 ttgatcgatc gattcaaagg gcatattcgt cattccgatt tcctgtcctt gataaataaa
    11881 tggcgtacct tgcaaaaaga aatacatcat agcgattgct tttgcagaca tagcccaaaa
    11941 atcgggacga gtatctccaa acactgaaac ggcacgagga acatcatggt tttccatata
    12001 taaagcgttc catccgatac catccaatga agtttgccaa gcgcttaatg catgtttcaa
    12061 tttaaggaca tctaattgcc cttctttttc ttgctgccag atatggatat gatcaaattc
    12121 gaatatcata tcgaaataac cgtctttccc tacccattgt ggtccttctt ctgcagtgac
    12181 accacttgct tctccgacag tcatgatatc gtactcttca aaaacatgtt tcaaatcggt
    12241 caaatgttct tcgattcctg agacattttg aaagggtgag aaagggttct ctgtggcttt
    12301 tactgaatat tcatcttttt ttacatgaga aatcgcatct aaacgaaatc catctatccc
    12361 tttatccaac caccatcgga tcatattgaa tagttcttct ttcaatttct cgctttccca
    12421 atttaagtct ggttgttctt ttgcaaaaac atgcagataa tactgtccag tttcatgaga
    12481 atactcccaa gcagaaccgc cgaaaatcga tgtccaatta ttcggttctt tgccatctac
    12541 cccgtcgacc caaatgtaat aatcgcgata cgggctttct ttagattttt tcgattcttc
    12601 aaaccattga tgctgatccg aactatggtt gatgacaaga tccataatca ctttgatccc
    12661 taattcatgt gcttctttta gcaataaatc aaaatcttcc atcgttccga atctctcttc
    12721 tatatcttga tagtcactta cgtcataacc attatcgaca aatggagatt gatagatcgg
    12781 attgatccag ataaagccga taccaagatc tttcaaataa ttaagttttt cacggatccc
    12841 attgatgtct ccttgaccat cgccattgct atctttgaaa ctagcaggat agatttgata
    12901 gcctactgct tctttccacc atttttgttc ttgtatcata tcgtttcact cctttaacgg
    12961 tccctcttga ttataaccga ttctatttta gaagtatcct tgttaccggt aactagaaac
    13021 gaaaagctca acgaatctgc taagtgcgtt cgttgagctt tattttatca tagatagcgg
    13081 gcaatcagtt gaatcatttg cttgggaaat tcattcaagt cagtaatatc tacaaaacga
    13141 ctttcaccgt agatcgactt gatttcttct ttgtcttggc caatagctgc ggagacaaat
    13201 aggattcctt gacgttcata ttctttcaaa acagactgga tatcttcttt tgcttttttg
    13261 cctgtataat ccggcaacgc ttttggctga ccgtcactaa tattaagcaa cagtttggtt
    13321 gttgcggatt gtctgtttaa tttttctgct gctaaacgaa gtgcagcacc atcacgattg
    13381 ttttgccttg gtttcatagt gactaatttt tcatttaacc attgaaatcc gtcttcaaat
    13441 tctttataag aaaacaaaga agtcttttct cttgacgaac ggtctgctgt atctccgtaa
    13501 atcaacaatg ggatatcgac tttcttagaa aattcggcaa tggcaatcgc tgcttttttt
    13561 gccgcttcga tccgatcatc ccgtatcatt gacccagatt catcgatccg tacagctacc
    13621 gctaaagaag gttgctcatg tggcgggttc tttttgtcaa aatttctcaa atcaccatat
    13681 gctactttgc ttgcattgaa ccgctgacct tcgtattttc ctttttggta cgtctctgtt
    13741 tgttcattat ccagtaactc taaaatttgc cggctaagtg attctactat tggcattact
    13801 tgcatgtgta gctcacgtgc ttccggcgtt tttccttgga aagctggtcg gtgaacgatg
    13861 agaccttctt tttcatgaat cgtttccttc atgatttgtc gcgcttcttt attcagttgc
    13921 ttcttgattt ccttatcatg tgcttccatg tcagaagcag acatttcttg agaatcatct
    13981 cctgtttgtt gttctttttg attttttcct tcgatatcgc tactgcgttc tttttgcatt
    14041 tgaggaggaa
//
LOCUS       DASJON010000060        13979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJON010000060 DASJON010000000
VERSION     DASJON010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.060"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..362
                     /locus_tag="U3989_001316"
     CDS             168..362
                     /locus_tag="U3989_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714773.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="U3989_001317"
     CDS             631..930
                     /locus_tag="U3989_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714774.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="U3989_001318"
     CDS             complement(1027..1461)
                     /locus_tag="U3989_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1714775.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="U3989_001319"
     CDS             complement(1693..2403)
                     /locus_tag="U3989_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1714776.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="U3989_001320"
     CDS             complement(2393..3253)
                     /locus_tag="U3989_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN1714777.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="U3989_001321"
     CDS             complement(3258..3896)
                     /locus_tag="U3989_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN1714778.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="U3989_001322"
     CDS             complement(3911..4210)
                     /locus_tag="U3989_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1714779.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="U3989_001323"
     CDS             complement(4253..5737)
                     /locus_tag="U3989_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1714780.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="U3989_001324"
     CDS             complement(5757..6218)
                     /locus_tag="U3989_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1714781.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="U3989_001325"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="U3989_001325"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN1714782.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="U3989_001326"
     CDS             7521..8291
                     /locus_tag="U3989_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1714783.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="U3989_001327"
     CDS             complement(8564..9391)
                     /locus_tag="U3989_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1714784.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="U3989_001328"
     CDS             9959..11194
                     /locus_tag="U3989_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1714785.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="U3989_001329"
     CDS             complement(12193..12453)
                     /locus_tag="U3989_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1714786.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="U3989_001330"
     CDS             complement(12480..13781)
                     /locus_tag="U3989_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1714787.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4519 a         2847 c         2187 g         4426 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaa
//
LOCUS       DASJON010000061        13839 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJON010000061 DASJON010000000
VERSION     DASJON010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.061"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            27..467
                     /locus_tag="U3989_001331"
     CDS             27..467
                     /locus_tag="U3989_001331"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1714788.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            595..1278
                     /locus_tag="U3989_001332"
     CDS             595..1278
                     /locus_tag="U3989_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN1714789.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1587..1660
                     /locus_tag="U3989_001333"
     tRNA            1587..1660
                     /locus_tag="U3989_001333"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1621..1623,aa:Arg,seq:tct)
     gene            1819..2178
                     /locus_tag="U3989_001334"
     CDS             1819..2178
                     /locus_tag="U3989_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN1714790.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2301..3932
                     /locus_tag="U3989_001335"
     CDS             2301..3932
                     /locus_tag="U3989_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN1714791.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3994..4635)
                     /locus_tag="U3989_001336"
     CDS             complement(3994..4635)
                     /locus_tag="U3989_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1714792.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4821..4897
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4917..5225
                     /gene="rplU"
                     /locus_tag="U3989_001337"
     CDS             4917..5225
                     /gene="rplU"
                     /locus_tag="U3989_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN1714793.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5252..5596
                     /locus_tag="U3989_001338"
     CDS             5252..5596
                     /locus_tag="U3989_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN1714794.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5609..5902
                     /gene="rpmA"
                     /locus_tag="U3989_001339"
     CDS             5609..5902
                     /gene="rpmA"
                     /locus_tag="U3989_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN1714795.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6336..9143
                     /locus_tag="U3989_001340"
     CDS             6336..9143
                     /locus_tag="U3989_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1714796.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9334..9825
                     /locus_tag="U3989_001341"
     CDS             9334..9825
                     /locus_tag="U3989_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1714797.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9928..10917
                     /gene="manX"
                     /locus_tag="U3989_001342"
     CDS             9928..10917
                     /gene="manX"
                     /locus_tag="U3989_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN1714798.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10950..11753
                     /locus_tag="U3989_001343"
     CDS             10950..11753
                     /locus_tag="U3989_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN1714799.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11774..12688
                     /locus_tag="U3989_001344"
     CDS             11774..12688
                     /locus_tag="U3989_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN1714800.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12771..13145
                     /locus_tag="U3989_001345"
     CDS             12771..13145
                     /locus_tag="U3989_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN1714801.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13284..13529
                     /locus_tag="U3989_001346"
     CDS             13284..13529
                     /locus_tag="U3989_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1714802.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13701..>13839
                     /locus_tag="U3989_001347"
     CDS             13701..>13839
                     /locus_tag="U3989_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEN1714803.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LE"
BASE COUNT         4408 a         2300 c         3008 g         4123 t
ORIGIN      
        1 ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata
       61 ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac
      121 cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa
      181 aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa
      241 aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta
      301 gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga
      361 ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct
      421 ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac
      481 ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat
      541 gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc
      601 aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag
      661 caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta
      721 ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct
      781 gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca
      841 atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct
      901 aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca
      961 gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa
     1021 tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg
     1081 atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt
     1141 ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc
     1201 gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca
     1261 gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg
     1321 gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga
     1381 aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct
     1441 tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat
     1501 aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta
     1561 taattattga tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct
     1621 tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact
     1681 gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa
     1741 taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc
     1801 agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt
     1861 gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg
     1921 gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac
     1981 caaggagtgt acaatggact attaggagtg ggactacttt atggagctta tctttcttcc
     2041 gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc
     2101 agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg
     2161 ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga
     2221 aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc
     2281 atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt
     2341 caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg
     2401 tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt
     2461 tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa
     2521 tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa
     2581 tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt
     2641 tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag
     2701 gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag
     2761 gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag
     2821 cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg
     2881 cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt
     2941 tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt
     3001 tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca
     3061 ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa
     3121 ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc
     3181 ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat
     3241 ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc
     3301 ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg
     3361 tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt
     3421 ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta
     3481 ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta
     3541 tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc
     3601 tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac
     3661 tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg
     3721 taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc
     3781 aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc
     3841 acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt
     3901 tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt
     3961 aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag
     4021 tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa tagctgctgg
     4081 atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat ccttttgttc
     4141 tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa ccgtatattc
     4201 tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt ttggctggac
     4261 cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac gagtaggtgt
     4321 ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat gagtcacgcg
     4381 atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta agccagttga
     4441 agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat gcgtatcaat
     4501 ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag gtccttgccc
     4561 agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt gtatcagttc
     4621 ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa aagaaacaca
     4681 atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat tgcttgaatt
     4741 tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct ggtaagctag
     4801 tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat aagcacagca
     4861 tatgctgtca cttcggatgg cgagtctaag tatatcagaa ggaggtgctt tatagcatgt
     4921 acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa gcaatctatg
     4981 ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt atcttagtag
     5041 gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg
     5101 ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct aaaaaacatt
     5161 cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg
     5221 cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta agcgaaatga
     5281 cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc cttatggaag
     5341 tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg ggattgcctc
     5401 attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt atctttatac
     5461 cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc tattagaaaa
     5521 tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc aaattaaaac
     5581 aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac aattatttgc
     5641 ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa aacgtctagg
     5701 tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc gtcaacgcgg
     5761 tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt tatttgcaaa
     5821 agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta
     5881 tccagtagct caagaagctt aattgaatct tatgattaaa gaggttgtga caagactttt
     5941 gtcacagcct tttttgttta taaaaataaa agttgtagtg gaggctacga agtttacttt
     6001 gtctttttac acttaatatt tttttgccaa tttggaatca ggatgttttg ataactatcg
     6061 ttttcaattg atggaagcta tattgtttgc tatcagtgaa ataattatat ggtaaacagt
     6121 gttctttttc ttgtttccat tataaaaatc gtatcgggat ttttcgttct tttaaattga
     6181 acatttctat ctatccgcgt gttatcaaca aaacaaaatc aggtaaatag ttgacgaaac
     6241 agaaataaat acatagtatt atgttttgaa gcctaagtac aatagtctat agaaaaggag
     6301 cgataccatt ggcaaaacgc attgaccgaa tttatatgta tgtcaaagaa aaaaccgctc
     6361 atttaacaaa ggcagaatat gatcaaggag taacaacaca ggaaattgct gaagtgttag
     6421 ggattcagcg aacaaatagc agtaaagatc tgaatcagct agtgcgcgaa ggaaaactga
     6481 taaagacaga cggacgacca gtacgttaca tatatcaaac actcgttacg cacgtaaagc
     6541 cagttacaaa acatgtagtc agttacaaag aaacgccggt ctctgaagaa aaaaatacac
     6601 tccaaataga tactaaggat atctttgcaa aaatcattgg agctaacggc agcatgaaaa
     6661 attcagtgga acaggcaaaa gctgcgattt tgtatccgcc aagaggatta aattgtttga
     6721 ttacaggacc taccggatca ggaaagactt attttgcaca cgcgatgtat cattttgcaa
     6781 aagcgcatca tgtaatagat gaggaaacgg aactgattgt tttcaattgt gctgattatg
     6841 ccaataaccc tgagcttttg atgagtcatt tatttgggta cgcaaaaggt gcttttacag
     6901 gcgcagatga agaaaaaatg gggattatcg atcaagcgga tggcggtatg ctgtttttgg
     6961 atgagattca ccggttgccg cctgaaggtc aagaaatgat cttttacttt atggatcatg
     7021 gaacttacag tcgtcttggg gaaacaacga aatcccatga agcaaatgta cggatcgtag
     7081 gtgcgaccac agaagaccca ggttcttctc tattagaaac ttttgttcgg cgtatcccaa
     7141 tcaatatcaa acttccggct tttgaaaaac gtcctgcaaa tgagaaagtc gatttagtaa
     7201 aaatcatgat tgcccacgaa gcaaaccgca cgcagcgaaa gatttctttg acagaagatg
     7261 tagcaaaagc attgatcggc agtgtgacct atggaaatat aggacaattg aagtcaaata
     7321 tccagttagt atgtgcacga ggatttctaa atcatatgca atcacccgaa atctcgatca
     7381 caatcgatga tttgacagaa gggattcgta gcggattgat tcaattagca agcaaccgca
     7441 gtgcaatggc agagctttct aaaatattgg agccaaagat aactgtagca ccaaatgata
     7501 cgatcatgaa aatccaatcg gactcctatg agttacctta taatctatat gatatcatcg
     7561 gagataaagc agctttactg aagtcggatg gtttggatca agaagcaatc aatcatttca
     7621 tttctacaga tatcaacatc catctgaagt ctttctataa agaccatggt ttttctttta
     7681 atgcagacag caaattagca gaatttgtag atccgaaaat catagaagtg accaatcaga
     7741 tttatgtaat ggtcaaaaga tctttaccat atgagtttca gcaaaacttt atttatgcta
     7801 tgagcttgca tatcagttct tttttgaaaa aaatcaactt aggagaagaa cggcatacga
     7861 atgataacat ccgtgagatg gcttttgatt ttccagatga atatgctgtt gcgaaagaag
     7921 tgcgtagtta tatcgaaacg tattttcaag tggagattcc agaaagtgaa gactattact
     7981 tgaccgttct actcgtctcg ttacgggcaa atcaagcaag cggcagaatc ggtgtagtcg
     8041 ttgcagctca cggaaacagc acagcaagca gtatggtcca agttgtccaa cagttgttag
     8101 atgcagagaa cgtacgagca gtagacatgc ctttggatat ggatccgaaa acagcattgg
     8161 accgaattga acgcaatgta caagaagtag atgaaggaag cggggtcata ctcttagtag
     8221 atatggggtc ccttgcgtcg tttaattcgc aaattcaaag agacactgga attccagtac
     8281 gtactgttga tatggtaaca acttctctag ttttggaaac tgtacgaaag gtctcagtat
     8341 tagggacaga tctagatatg ttgcatgaat cattgaaaaa ttttcgaggt tacgcagaaa
     8401 tatcttctgt agaatctgtt caggaagcaa aacctattca taagaaaaaa gctgttttgg
     8461 ctatctgcgc atctggcgaa ggtacagcac aaagaatcaa agaattgatc gaacgagcgg
     8521 taaacaagcg tcaagaaaca gaattagaag ttctagcttt gtcgattgtg gaagtgaaag
     8581 aagaactgcc agttatccaa aaagaatatc agatcatagc tactactggg attactgatt
     8641 tgaatatcca agccccattt attcctttag aacgttttat tgatcaaaat atcgaagtca
     8701 ttttggatca gttgctgatg gaatcagaat tggaggaaac tgaattcatc tccctagatg
     8761 aagagtcagc taaaaatacg tgtgttgagt ttatctctga taacttcatc tttattaatg
     8821 gtagtaagtt aatcgatcca atgtggcaat tttctacaca aatctctcag acgacgggaa
     8881 tcggcgatga agaatatgga ttcaaaatca acctagtgat gcacacagca ggaatgatcg
     8941 aacgtattat tcggaatgaa ccattgactg tggaagaaaa cgaattgaca aatacaacga
     9001 atgatcctct ttatagccaa ttggcagcct cagttgttct gttggaagat cagataaaag
     9061 taaaagtgcc gatagaagaa atgtattatc tgctacgtct agtccataat cagttagata
     9121 aaaaagaata cactgtgcca tgaatacaca aaaacgatac acttttatag tgtatcgttt
     9181 ttgtgtattg gttaagaatc ctttaaattc aattagtgta tcgtaaaaaa gttggcacgc
     9241 tttttgctta tagtagtgtg tgctaggagg tggttgttca cacagaaatg ataggggttt
     9301 ctgtgaagta gtgaggaaga aggagttttg cagatggata ttcgattggc acgtatagat
     9361 gatcgtttaa tccacgggca agtcgcaaca gtttggtcga agcaaacagg tgttgaacgt
     9421 atcattgtga tcagcgatgt agtcgctcaa gataaattga gaaagtttct actaaaagaa
     9481 gctgcaccgc cggggatcac agcaaatgta ataaccgtgc agaaaatgat tcaaatctat
     9541 catagtggat tattatcacg agcaaaagtg atgcttttat tcaccaatcc ccaagatgtt
     9601 gaagcggttg tccgctgcgg tgtacagttg aagtcgttga atatcggcgg tatgagtttt
     9661 actgaaggca aatcaatgat taccaatttt gtttcagtaa atcaacaaga tattgattcc
     9721 tttcgttact tgaacagtca agaagtggag ttagagattc gaaaagtacc tgctgacagg
     9781 aaaatccttt tgatggaact actagcgaaa atcaaaaagg tgtagcaaga atggttgttg
     9841 gaaaaggtca aataattctg ttgtaatggt tagcaattat taatattttt gaatttgtta
     9901 caacaaaagt aaataggagg tataaatatg gtagggatta tcctagcaag tcatggacaa
     9961 tttgctgaag gaatcttaca atccggttca atgatctttg gcgaacaaga aaacgtcaaa
    10021 gccgtgattt tgaaaccaag tgaaggtcca gatgacttga gagccaaatt ggaagaagcg
    10081 gttgcttctt ttgataatca agacgaagtg ttattcttag ttgatttatg gggcggaaca
    10141 ccgttcaatc aatcaaacac attattcgaa gagcacaaag ataaatgggc aatcgtcagc
    10201 ggtttgaatt taccaatgtt gattgaagcc tacgcatcac gcttttcaat ggaatctgct
    10261 catgaaattg cagcacacat cattgaaaca gcaaaagacg gtgtaaaagt aaaaccggaa
    10321 gaattagaac cggcagaagc accgaaagca gcagttgaag acgcacaacc taaaggagca
    10381 cttccagaag gaactgtggt cggagacgga aaaatcaaat atgtattagc acgtgttgac
    10441 tcacgtctgt tacatggaca agttgcgaca gcttggacaa aagcagtgca accaaaccgt
    10501 attatcgttg tttcagatgc agtatcaaag gatgatctgc gtaagagact aatcgaacaa
    10561 gctgctcctc caggtgtaaa agcaaatgtt attcctatta gcaaaatgat cgaagtagca
    10621 aaagaccctc gttttggaaa taccaaagca ttgcttttat ttgaaaatcc tgaagacgta
    10681 ttgacagctg ttgaaggtgg cgtagacatc aaagaattaa atgttgggtc aatggcgcat
    10741 tcggtcggta aagtcgttgt aagtaaagta ttgtctatgg gacaagaaga cgttgaagcg
    10801 tttgagaagc ttgaacaaaa aggtgtgaaa ttcgatgtac gtaaagtacc taatgattca
    10861 cgcgataaca tggatgatat tttgaaaaaa gcaaaagctg aattagcaaa agcgtaataa
    10921 ataatattaa aatttatagg aggcttatca tgtctattat ttcaataatt ttagtcgtat
    10981 tagttgcctt tctagctggt atggaaggta ttcttgatga atttcaattt catcaaccac
    11041 tagtggcatg tacattgatc ggtttagtta ccggtaacct cgaagctggt atcgtacttg
    11101 gcggtacgct tcagatgatc gctttaggtt gggcaaatat cggtgccgct gtagcaccag
    11161 atgctgcatt agcatcagta gcatcagcaa ttattttagt tttaggtgga caaggtgtta
    11221 aaggtgttcc ttctgcgatc gcgattgccg ttcctttggc tgttgcaggg ttgttcttaa
    11281 caatgatcgt acgtacaatc gctgttccaa tcgttcattt gatggatgcg gctgctgaag
    11341 aaggaaacat ccgtaaggtt gaaatgtggc acatcattgc tgtatgttta caaggggttc
    11401 gtatcgcggt cccagcagca gcgttgctat ttatcccagc acaaactgtg caatcattcc
    11461 ttgaatcaat gccagcatgg ttaacagatg gtatggctat cggtggggga atggtcgtag
    11521 ctgtaggtta cgctttggta atcaacatga tggctacaaa agaagtatgg ccattcttcg
    11581 ttattggttt cgttgtagct gcaatttctc aattaacatt gattgctcta ggtgctttag
    11641 gtgttgcatt agctcttatt tacttgaacc tttctaaaat gggtggttca tcaaataatg
    11701 gaggcggaag caactctggt gaccctttag gcgacatttt gaatgactat taatcttgaa
    11761 ggaggaaaat aaaatggcag aagaaaaaat caaattatca aaaaaagatc gtttagctgt
    11821 tgcgtggcgc tctaccttca ttcaaggttc atggaactac gaacgtatgc aaaacggtgg
    11881 ctgggcattt tctatgattc cagcaatcaa aaaattatac aaaacaaaag aagaacgttc
    11941 tgcagcatta aaacgtcact tggaattctt taatacacac ccatacattg cttctccaat
    12001 tttaggggtt acattggctc tggaagaaga acgtgcgaat ggcgcgcctg ttgatgatgt
    12061 agcgattcaa ggggttaaag taggtatgat gggtccttta gctggtgttg gtgacccagt
    12121 gttctggttt acagtacgtc caatgctagg tgcactaggt gcttcattag ctatgggagg
    12181 aaatatcctt ggaccaatca tcttcttctt aggttggaac attattcgtt gggcattcat
    12241 gtggtataca caagaatttg gctacaaagc tggttctaaa attactgatg acctttctgg
    12301 cggattgtta caagacgtaa caaaaggtgc ttcaatttta gggatgttcg tattagctgc
    12361 ccttgtacaa agatgggtat caatcaaatt cttaccagtt gtatcaactg taaaattaga
    12421 taaaggtgct tacatcgaat gggataaatt gccttcaggt ggtgaaggaa tcaagagtgc
    12481 atttgaacaa gttaataatg gcttagccct ttctcctgaa aaggtaacaa cattacaaga
    12541 caacttagac caattgattc ctggtttagc tgcattagga ttaacattgt tatgtatgtg
    12601 gttgcttaag aaaaaggttt ctccaatcgt tatcatcctt ggtttattcg tagtcggtgt
    12661 tgttggacac gtcatcggac ttttataatc cataaaaata tagctcgggc gttgtcccga
    12721 gcttttttta tatagtatac tgttcataaa caaatatctg gagggagaaa atggttcaat
    12781 caatcaatac aaaagtagac ttggtaattg atgctacttc gtttacgggt ctgacagatt
    12841 acgggcaaat catgataggt aataatggtt ttgagtttta ccatgcgcga gatagacgca
    12901 aatatatcca aattccatgg gaagaagtgg attatgtact agcatcagtc atgctaaagg
    12961 gaaaatggat tcctcgatat gccattcaaa cgaaaaaaaa tggtacatac acattttctt
    13021 ccaaagaacc taaaaaagtg cttcgtgcga tcagagaata tgtagatgaa gatagaatgg
    13081 tacaatcatt gagtttcttt gatgtagtga aacgtggtat tcgtagtatg tttaaacgca
    13141 agtaaaaaat aaagaaaact tctaagagga ggttttcttt atttttttac tttgcagaag
    13201 aaaatatgaa atagcaaaac caattatttg tcccctttaa atcccatatg ctaaaattga
    13261 ttagcaaaga aggaggttat attatgagtt ggttatggac attgattgtt ggagccgtga
    13321 tcggagcaat tgcaggtgcg atcaccagta aaggaaattc gatgggctgt gtaacgaata
    13381 tcattgctgg actagtcggt tcaagtattg gacaagcgat tttcggcgaa tggggaccaa
    13441 aagctgcagg tatggcactt attccatcta ttttaggcgc agtgattttg gtagcagttg
    13501 tctcattttt ctttggaaaa cgatcataag tttttacaaa atagtaaact aaaaggagtt
    13561 gcagcgagac tgtgactcct ttttggcttt atttatttaa aataaaaaga agttttccgg
    13621 aaatttttat ttaactgtcg tgtacgaaga aaagtttgct ataatggaaa aaagcgtgca
    13681 aagaaagagg gagtttcatt atgatgctac gtgtagaaaa attgcgcaaa aaaatgcaag
    13741 aagaaaattt ggattcattt ttggttacca gtccgtacaa tcttcgttac ttaacgaact
    13801 ttacaggtac gacaggatta gctgttatta ctttggaaa
//
LOCUS       DASJON010000062        13794 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJON010000062 DASJON010000000
VERSION     DASJON010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.062"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="U3989_001348"
     CDS             140..460
                     /locus_tag="U3989_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714804.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="U3989_001349"
     CDS             912..1112
                     /locus_tag="U3989_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1714805.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="U3989_001350"
     CDS             1259..4048
                     /locus_tag="U3989_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1714806.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="U3989_001351"
     CDS             4095..4622
                     /locus_tag="U3989_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN1714807.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="U3989_001352"
     CDS             4643..5833
                     /locus_tag="U3989_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1714808.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="U3989_001353"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="U3989_001353"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN1714809.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="U3989_001354"
     CDS             7966..8640
                     /locus_tag="U3989_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1714810.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="U3989_001355"
     CDS             8637..8978
                     /locus_tag="U3989_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714811.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="U3989_001356"
     CDS             9008..9367
                     /locus_tag="U3989_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN1714812.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="U3989_001357"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="U3989_001357"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1714813.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="U3989_001358"
     CDS             12286..13752
                     /locus_tag="U3989_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1714814.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4579 a         2392 c         2938 g         3885 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct actt
//
LOCUS       DASJON010000063        13758 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJON010000063 DASJON010000000
VERSION     DASJON010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.063"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(66..770)
                     /locus_tag="U3989_001359"
     CDS             complement(66..770)
                     /locus_tag="U3989_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1714815.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            901..1404
                     /locus_tag="U3989_001360"
     CDS             901..1404
                     /locus_tag="U3989_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN1714816.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            1364..1702
                     /locus_tag="U3989_001361"
     CDS             1364..1702
                     /locus_tag="U3989_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN1714817.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            1723..3141
                     /gene="ffh"
                     /locus_tag="U3989_001362"
     CDS             1723..3141
                     /gene="ffh"
                     /locus_tag="U3989_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN1714818.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            3253..3831
                     /locus_tag="U3989_001363"
     CDS             3253..3831
                     /locus_tag="U3989_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN1714819.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            3835..4356
                     /locus_tag="U3989_001364"
     CDS             3835..4356
                     /locus_tag="U3989_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1714820.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(4435..4989)
                     /locus_tag="U3989_001365"
     CDS             complement(4435..4989)
                     /locus_tag="U3989_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1714821.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            5242..5517
                     /gene="rpsP"
                     /locus_tag="U3989_001366"
     CDS             5242..5517
                     /gene="rpsP"
                     /locus_tag="U3989_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN1714822.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            5529..5780
                     /locus_tag="U3989_001367"
     CDS             5529..5780
                     /locus_tag="U3989_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN1714823.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            5905..6696
                     /locus_tag="U3989_001368"
     CDS             5905..6696
                     /locus_tag="U3989_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714824.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            6866..7387
                     /gene="rimM"
                     /locus_tag="U3989_001369"
     CDS             6866..7387
                     /gene="rimM"
                     /locus_tag="U3989_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN1714825.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            7377..8138
                     /gene="trmD"
                     /locus_tag="U3989_001370"
     CDS             7377..8138
                     /gene="trmD"
                     /locus_tag="U3989_001370"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN1714826.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            8274..8621
                     /gene="rplS"
                     /locus_tag="U3989_001371"
     CDS             8274..8621
                     /gene="rplS"
                     /locus_tag="U3989_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN1714827.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(8753..9889)
                     /locus_tag="U3989_001372"
     CDS             complement(8753..9889)
                     /locus_tag="U3989_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1714828.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(10005..10571)
                     /locus_tag="U3989_001373"
     CDS             complement(10005..10571)
                     /locus_tag="U3989_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEN1714829.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(10707..11360)
                     /locus_tag="U3989_001374"
     CDS             complement(10707..11360)
                     /locus_tag="U3989_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714830.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(11410..11838)
                     /locus_tag="U3989_001375"
     CDS             complement(11410..11838)
                     /locus_tag="U3989_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1714831.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(11843..12217)
                     /locus_tag="U3989_001376"
     CDS             complement(11843..12217)
                     /locus_tag="U3989_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1714832.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            12521..12706
                     /locus_tag="U3989_001377"
     CDS             12521..12706
                     /locus_tag="U3989_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1714833.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            12719..12967
                     /locus_tag="U3989_001378"
     CDS             12719..12967
                     /locus_tag="U3989_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714834.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            12998..13168
                     /locus_tag="U3989_001379"
     CDS             12998..13168
                     /locus_tag="U3989_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714835.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(13270..13728)
                     /locus_tag="U3989_001380"
     CDS             complement(13270..13728)
                     /locus_tag="U3989_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714836.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
BASE COUNT         4487 a         2432 c         2647 g         4192 t
ORIGIN      
        1 gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat aaattttttt
       61 ccatttcatc gttttggttc ctgaaactta tagccaaatc ctctaatagt caccaagtac
      121 tttggatgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg tacatctacg
      181 attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg
      241 atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt cggtgttagt
      301 tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt gatttgtcca
      361 atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc aatccgtctg
      421 aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt caaatagtcg
      481 tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc cgtcagcatc
      541 aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat gccatccatc
      601 gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc taattctaat
      661 ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa attgaacgtc
      721 aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat tcaaatctcc
      781 tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag ggctatttta
      841 caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga agagcagtca
      901 atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa
      961 gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt ctgtccagaa
     1021 gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg aaatggattc
     1081 gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg
     1141 tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat tgatctagtc
     1201 atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg aagtttttcc
     1261 ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca aaaaatgact
     1321 gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa tcgaaaagac
     1381 caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa
     1441 ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg aagaatacga
     1501 agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct tggaggatta
     1561 tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat tggaaaatat
     1621 gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat atgtacagca
     1681 aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt tgaaagctta
     1741 actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct
     1801 gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga tgttaacttg
     1861 caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg
     1921 gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt gacgatcaca
     1981 cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt tatcatgatg
     2041 gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa ttatttaaag
     2101 aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc agctgcgatc
     2161 gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat gggaactgaa
     2221 gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac
     2281 tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat ggatgagttg
     2341 aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga tgccatgact
     2401 ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat tacaggggtc
     2461 gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat ccgctcagtg
     2521 acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt agaagtattc
     2581 cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac attgattgaa
     2641 aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa
     2701 aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg tatgggccca
     2761 ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat cgaaaacgtc
     2821 aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat gacgccagca
     2881 gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc tgccggttct
     2941 ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg
     3001 atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat gtttggttca
     3061 ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa aaataagaag
     3121 aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg aaaatgtcta
     3181 ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac tctaaacgaa
     3241 gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg gcaatatcat
     3301 gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa attgatgctt
     3361 gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga agcattcgat
     3421 gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt
     3481 gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc agccgtccaa
     3541 aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga gtatctttgg
     3601 tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga agtcccaaca
     3661 acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt taaatttatc
     3721 ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga tcaccttgac
     3781 agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata aagcatgcga
     3841 aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa aacggatgat
     3901 cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc tttttatgaa
     3961 acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa atatttggag
     4021 accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc acaattacaa
     4081 ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg
     4141 acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa
     4201 catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg ggacgggatt
     4261 cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac gacacgattc
     4321 ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata aaaaaggcaa
     4381 ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg ctttttactc
     4441 tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga aacttaatgt
     4501 tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga tatatgcttc
     4561 cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc
     4621 gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt tatgatccca
     4681 aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata ctgctggcac
     4741 agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca cttcctcacg
     4801 aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat tgtaaaatgg
     4861 cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt tcgcaaaagc
     4921 ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa atccaatttt
     4981 ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa aagataagta
     5041 actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat tacttatttt
     5101 taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt aatcaagctg
     5161 taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt gtgaataaaa
     5221 caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat gggttctaaa
     5281 aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc
     5341 atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt tttaaaagaa
     5401 gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt acgtaacatc
     5461 ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa gaaataaggt
     5521 gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg ctagtcactt
     5581 atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag tacaatctaa
     5641 cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa
     5701 tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca
     5761 tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt
     5821 gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt
     5881 ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc atttgctcta
     5941 ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct atcaagcagt
     6001 atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc caccactgaa
     6061 caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt gaaagaacaa
     6121 cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct caatatcgca
     6181 gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg tcccctcggg
     6241 ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata tcaatatggg
     6301 tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga cacaaatggt
     6361 cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt
     6421 tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa tatagaaggc
     6481 caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa cagtctacca
     6541 gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa tcgagccgta
     6601 gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct
     6661 gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga tctgagccca
     6721 tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt ttgcataatt
     6781 ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta aaataaaaca
     6841 gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa
     6901 cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc cagaagaacg
     6961 ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg tggaattaac
     7021 agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag gacatccttc
     7081 tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag acaactctgt
     7141 agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg tattagaaga
     7201 tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg atgtttgggt
     7261 cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg tcgtcaaaga
     7321 agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga ttgacgatga
     7381 agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg gaatccatca
     7441 tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc cgcgattatt
     7501 cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca ggtatgctgc
     7561 tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca ccggatgtca
     7621 aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa atggcagaag
     7681 aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga tatgatgaac
     7741 gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt actgggggag
     7801 aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat gtgttaggga
     7861 atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat ccccaatata
     7921 ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat ggaaatcata
     7981 aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa cgtcggcctg
     8041 atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa gaaatcaaga
     8101 aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa tcttctttac
     8161 agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa ctattacgat
     8221 attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa accatgaatc
     8281 cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg ttccgccctg
     8341 gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat
     8401 ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat acagtacgta
     8461 aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca cgtgttgcaa
     8521 aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat
     8581 tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcatcgtta tcagacagag
     8641 aaagaaccct tggtattcaa gggttctttt tttattatct gtttttttat attaacgatt
     8701 tctatttggt acatttttga ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt
     8761 tttctatatc ttctttcaat ttttctttta ctccttttgt cacatgcaaa taaatttttt
     8821 cagtgatatc actattttcg tgaccaacgc gatcctgaat ggcatacaga ggagttccta
     8881 gttccgctaa ttttgaaata tgggtatgtc taaaaatatg tgaactaagt tttttatcaa
     8941 tcttcatgtc agctttatga cttctcaaat aggtgttaat tgctgttagt tggaaaggag
     9001 ttcctttcgt tgtttgaaat agaaattgac catttggatt taattctaga agctcattgt
     9061 aaatagctat cgcttttttt ggtaaatcaa tttctcgcat tccggcagct gtttttgtag
     9121 aatcagattt tttcatatca gctattgaac gttctctata catcatcgtc ccatttataa
     9181 ctactgatgc attattattg gtaatgtgta catcatcttt acttaatgcg attgcttcac
     9241 cagacctcat cccagtcaaa tatagccact ggaaaaggag agaatatctt ttattgtgtg
     9301 aagttgtgaa atctactagt ctattatatt catcatcttc taaaaattta tctttaattt
     9361 ttatcgtttt tgactctctt ttataatcaa taactacttc atcgatagga tttttttcta
     9421 cataaccttt tttcatagca tatgaaaaaa gaagattcaa tttggattta attacactga
     9481 cgtacttatt tgacaagtca tctttatata tcatgtcttc aaaaatatta ttcaaatcta
     9541 ttgtattaat accagaaacg atgtaagtct caggtatctt cttctttata gtgtttaata
     9601 tattattcgt aggataatat gtagattcct taacttgtct tttgtatata actaaccatt
     9661 cttcaacaag ctcatgaaat gttagatcag gcttatgaag tgttttttcg tttaatttta
     9721 tatcaatttt tttatttaac tctagcaatg ccactttctg agtttctcga gatttatttt
     9781 tataagtaat gctcactttt tttctttttc tagtttttgg atcaatatat cgctcattat
     9841 atttatatac tttttttccg tttttatcag ttttagtttc gatccacatt gttttcatct
     9901 cctatttgtt gctataatag gaatagataa gtaagccgat tatagcaggt ttatttttca
     9961 tcacgtccac aaactttggc gagggagggg cgtgtttttg ttagttattg tggtaggtta
    10021 tctaaagcgt attgtgcttg ctcatttgta aaaccttcaa atataagttg atcgtacaat
    10081 tgcgaatctg acattgatgc ccaatcatcg taactttccg ctttagccaa agcttgttcg
    10141 ttgtaatcga catcaacatt agccagcgca taatcgattg catcacttgg atattcttca
    10201 aactctagtt gttcacgcaa accttgttca gacataccag cccaacctag atagctttca
    10261 gctttgctca atgcatttct gtattcacga ggaacacttt cttcttgagt aacttcttct
    10321 atagtagttt cagtagtttc agtttcttct tcaacgactg tagattctgc taccgattct
    10381 gcagaagaca cttctgtcac gctttcttta gttgtttcag cgacggtatt tttagcatca
    10441 tcactgcctc cttgcgaacc tatagcaata aacaccacaa caaccgctaa caaccaaaac
    10501 catacccgtt tataaaacgg tttcttaact ttatacattt tcccatcttg acccataact
    10561 ttttttgcca tttaaatatc cctcgcttct tgttataata tatttgtgat ctcagaaatg
    10621 aggtatgagt ccgtgttgca gcacgggctt ttttctttat aactttttta gagattatag
    10681 gcaaaatagt agggcataaa aatgtattat tgaattccgt atttagaaaa tcctaattga
    10741 acttcaccgg aagtcttttg ctgtgttgta cgcaatgctt cttcttcaga cattccattc
    10801 tgtactttcc atgcaacagg cgacatcccg tatttgttaa caaaatcagt aagtgataaa
    10861 gtgtcagcgt cttgctgagc gcttgtttgt tggtcttctg gattctgttg agatgctgct
    10921 tgttgttctt tctgatcttg actgataata ttgccagcat catctgtagt caatccattt
    10981 tcataaaggg ccacgccgaa agcttcccac tctttgttgg accaatttgc acgatcagct
    11041 ggagttgact gtaaagtgcg ttgtttcatc tgttcatatg tttcttcttg aggtgcggtt
    11101 tggattgtac tctgactgga gcttataact gttgggctag gttccgccgt agcttggttg
    11161 gagcttgtaa ctgttgaact agaatctgtt ttagatgtag atttgctagt agatgaactg
    11221 gtttcagttg tttcttttgt tttacttatt tttgtttctt ggttagaagt ggtatctgtt
    11281 gattcagctt ttttattatt tgaacaagct gaaagtagca gagcagtact taacaacaac
    11341 ataacgctaa cttttttcat tttataattc ctctttctcg ttgtaatatg tgtgctaaca
    11401 cgggcttttt tatataagaa aacgataagc gctttctgga agcccgtaaa gattctttaa
    11461 ttcctcgatt tttttaggat attgatcatt atcttcttta taaagagaaa caatgagatt
    11521 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    11581 atagctggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    11641 tctttcctca gagtctcgca gttcatcatt taaaaagatt atcgcacgat ctctaatttc
    11701 ttgaaactgc cccttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    11761 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    11821 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    11881 tatcccagaa agtcgctatc aggatatctt ttacgcgctg tatttgttcg ggtgtcaatg
    11941 tttccccacc ataagacata ttaacatttg agtctagtag tttatcaagt tcaaccactt
    12001 cctcttttgt agcccacttg ggaacattat tatttcccaa taaataatca gttgtgacac
    12061 caaaataatc agcaactttc tttaagttct cagattttgg cgaggcttta tcccatcttc
    12121 ttatttgtcc attagagatg cccacctgtc tttctacttc tgctatagtc acatgctttt
    12181 cgtctgctaa ttctttaatc ttagtaacta aactcattat tatcaacctt tcaaagctga
    12241 aaagaaaata aatagcttaa aagttatatt tttggttgac aattagcttt taagctagta
    12301 tatttaattc gtaagctaaa ttgttagcta aataagagca acaaaaaact ctactaattt
    12361 aaaacattct ctcggtcgcc aaacttagaa atgttatttt agaggctttt tataagtctt
    12421 atttaactat gtattcataa tagcttaaaa gctaataggt gtcaacgatt tagctaattt
    12481 tttagcttac aaattatttg tttagaaagg agctattttt atgtctgaga atttagactt
    12541 aaaaattcga gcggagatga gaaaaagaag aatgactttc aaagaactag ctgcgcttat
    12601 cggtatttca ggagcttatt tatcagatat tctaaacggc aatcgtgatg gaaagaaagc
    12661 acaacagcat atcgaaacag tgaaaaaaat attggacatc cgataggggg ggtaggcgat
    12721 gacaaaacta aaaaaacaag attttgtaaa aaaatataat tattctccat ctacttatca
    12781 acgtcgaatg tcggaactaa aaaatacagc aattttctca gcggcgtatg aacgggtcac
    12841 aggacaagaa gtttggatca atacagaatt atacgataaa tttttgtctt tcaaatccta
    12901 taacaggtta cgcacaagaa aggtaacacc taaagaattt atcgagaagc atttagttga
    12961 tttataaaaa acagaacatt ttgagaggtg aaggttaatg ggtaaattca acagagcatt
    13021 agtattcagc gcaccgctaa tcgtctatgc tttagggctt tggggaagta gacaagcgtt
    13081 gataggaacg attgtgtaca tggtttggat ttttataggg ctggatgaag ctgagtacaa
    13141 aacaaaaaag ccagtcggga gggactgact aatgagtaag gacgatattg tatttctgtt
    13201 aattatgttt tgtgggattg gatcaataat aattaatttg tatttgatta ttaaagaaaa
    13261 atttttcaat taaaatgagt cttttagctt taacttaaat tttttttgat aattagctgt
    13321 tcttatgacc attacaagtt tttcaccagg taatatatct ttcatatcta aaacttctga
    13381 aaaagcgact gagtagccag gagagattgc ttgtgccaac ggtataggag ataaaaggct
    13441 acttttatga atgcttccca tatcaaaacc attggcgtga taatgtgaac aattaatttt
    13501 cttaccgttg taaagatacg tacattcgat aattgagttt ggcatttttg atgggtttgc
    13561 aattacgcca gtaagaatca ttcgattact tctatcatgc ttaactaaaa aataggagag
    13621 cgtaattttt cttttatctt taaataagtt atagagggat aaaaaaaatc ctgaaataga
    13681 aatgattaaa gtaatagcga cttggtaatc gctcaaaatt tttatcaagt aattcaccac
    13741 ctttttactc aaattata
//
LOCUS       DASJON010000064        13732 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJON010000064 DASJON010000000
VERSION     DASJON010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.064"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(92..649)
                     /gene="frr"
                     /locus_tag="U3989_001381"
     CDS             complement(92..649)
                     /gene="frr"
                     /locus_tag="U3989_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN1714837.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(652..1374)
                     /locus_tag="U3989_001382"
     CDS             complement(652..1374)
                     /locus_tag="U3989_001382"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN1714838.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1510..2391)
                     /locus_tag="U3989_001383"
     CDS             complement(1510..2391)
                     /locus_tag="U3989_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN1714839.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="U3989_001384"
     CDS             complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="U3989_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN1714840.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3631..4110
                     /locus_tag="U3989_001385"
     CDS             3631..4110
                     /locus_tag="U3989_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN1714841.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4327..5418
                     /gene="phnW"
                     /locus_tag="U3989_001386"
     CDS             4327..5418
                     /gene="phnW"
                     /locus_tag="U3989_001386"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN1714842.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5411..5539
                     /locus_tag="U3989_001387"
     CDS             5411..5539
                     /locus_tag="U3989_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1714843.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5543..6088
                     /locus_tag="U3989_001388"
     CDS             5543..6088
                     /locus_tag="U3989_001388"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1714844.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6542..8233)
                     /locus_tag="U3989_001389"
     CDS             complement(6542..8233)
                     /locus_tag="U3989_001389"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN1714845.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="U3989_001390"
     CDS             complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="U3989_001390"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN1714846.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9715..10734)
                     /gene="argF"
                     /locus_tag="U3989_001391"
     CDS             complement(9715..10734)
                     /gene="argF"
                     /locus_tag="U3989_001391"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN1714847.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="U3989_001392"
     CDS             complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="U3989_001392"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN1714848.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12515..13216
                     /locus_tag="U3989_001393"
     CDS             12515..13216
                     /locus_tag="U3989_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1714849.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3884 a         2785 c         2449 g         4614 t
ORIGIN      
        1 taattttttc tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg
       61 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
      121 cagcagcgat cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt
      181 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      241 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca
      301 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      361 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      421 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      481 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      541 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      601 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      661 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      721 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      781 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      841 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      901 gataacatct gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta
      961 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
     1021 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1081 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1141 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1201 ccagatattt ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1261 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc
     1321 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1381 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1441 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1501 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1561 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1621 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1681 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1741 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1801 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1861 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1921 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1981 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     2041 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2101 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2161 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2221 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2281 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2341 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2401 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2461 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2521 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2581 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2641 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2701 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2761 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2821 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2881 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2941 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     3001 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3061 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3121 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3181 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3241 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3301 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3361 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3421 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3481 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3541 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3601 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3661 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3721 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3781 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3841 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3901 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3961 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     4021 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4081 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4141 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
     4201 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
     4261 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4321 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
     4381 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4441 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4501 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4561 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     4621 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4681 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     4741 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     4801 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     4861 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4921 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     4981 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     5041 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5101 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     5161 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5221 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5281 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5341 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5401 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5461 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa cccaactgac
     5521 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5581 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5641 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5701 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5761 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5821 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     5881 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5941 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     6001 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6061 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     6121 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6181 aagaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     6241 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6301 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     6361 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     6421 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6481 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6541 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6601 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6661 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6721 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6781 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6841 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6901 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6961 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     7021 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7081 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7141 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     7201 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7261 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7321 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7381 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7441 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7501 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7561 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7621 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     7681 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7741 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7801 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7861 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     7921 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7981 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     8041 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8101 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     8161 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8221 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     8281 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8341 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8401 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8461 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8521 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca
     8581 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8641 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8701 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8761 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8821 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8881 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8941 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     9001 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9061 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9121 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9181 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9241 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9301 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9361 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9421 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9481 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9541 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9601 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9661 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9721 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9781 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9841 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9901 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9961 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
    10021 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10081 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10141 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10201 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10261 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10321 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10381 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10441 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10501 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10561 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10621 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10681 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10741 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10801 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10861 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10921 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10981 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    11041 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11101 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11161 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11221 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11281 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11341 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11401 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11461 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11521 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11581 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11641 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11701 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11761 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11821 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11881 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11941 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    12001 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12061 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12121 ttttgtctca ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa
    12181 cgtttttcta tttttaagcg taatttattc attttttatt ctgtatacac tttcaaaaaa
    12241 atatttattc attgaaaaaa tttttttgaa taaattaaaa aaataaatta tattatcgtt
    12301 gttagcgttt actatatgta ttattgttgt tttttataca caaatcattc gcttcaaaac
    12361 aaattattca ataccattgt atatttttta cataataaca aattatattt gtaaagtatt
    12421 aaaacttgtt tctaatgttg gtatgttaaa actttacaaa ttctttaaaa aaagtgacaa
    12481 taaatatagt gaacaataag gagaggactt tttaatgcgt gttaataaaa tcgattttca
    12541 cttttcaaaa ctcagatatc aacctgattt tgaacatttc tcggatgaag agttccaaca
    12601 gatcaaggaa catactgttc ttcgttctta taaaaaagga cagatccttt tcgacgatgg
    12661 tgatcccaga caacggttgt atatcttgac caaaggactc gttcgtttag aacgttatga
    12721 tgaaagtgca acttattact actatgacta tgtgaataca aataaccttt ttcctatgtc
    12781 aggaatgttt ggagaagaga cctacactta tacagctcaa gcgatgactg atattgaaac
    12841 attctacctt cctgtttcct actatgaaaa attagcacga aaaaatcccg aacagttgat
    12901 ctatatgata caaaaaattt ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg
    12961 attgacctct agcgcctttg accgggtaaa acaaaattta ttcattttga aagaagaatt
    13021 gggcgtacca acaaaagatg gtcatatcac cattccttat ccgatcacct taaaagaatt
    13081 agcagtgaat agcggcacga cacgtgaaac tgctggacaa gtaatcaaac aactacgtga
    13141 ttccgggctg ttagattatg acaaaaagcg ttttactttc tgtaagacag ctactactcc
    13201 agctatgaga gaataacgcg aacaaaaata agcagtgacc ctgataaata tcagggtcac
    13261 tgcttatttt cctccctaaa acttctgaaa gatccggcag ttttagatca ctggacaaga
    13321 ctcaaagatt ttctatgtgt gtttcagtta tgtaatattc actgtttgca aaaaatactg
    13381 ttagattaac tagatgaagc gtcttgtcct catccgatct agattattcg atattcctcg
    13441 acaataaacg attactcagt gagggcaatc agaggaacgg ataatgataa ataagctgta
    13501 aaaaatagca aaataacaat aatgattaaa agtatgtaaa aaatagggct ttgacaaaaa
    13561 cgtgtcacag ccctatttcg attaaacggt gtgtacctgt caactcttcg gtcacaagtc
    13621 ataatgttca taaacatgag gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa
    13681 acgacaggct tacgaactct tgattaaacg gcgttcaaaa gcttgcatac gc
//
LOCUS       DASJON010000065        13258 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJON010000065 DASJON010000000
VERSION     DASJON010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.065"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1875)
                     /locus_tag="U3989_001394"
     CDS             complement(<1..1875)
                     /locus_tag="U3989_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1714850.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     R"
     gene            complement(1890..2417)
                     /locus_tag="U3989_001395"
     CDS             complement(1890..2417)
                     /locus_tag="U3989_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEN1714851.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            complement(2392..2865)
                     /locus_tag="U3989_001396"
     CDS             complement(2392..2865)
                     /locus_tag="U3989_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1714852.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            complement(2856..4205)
                     /locus_tag="U3989_001397"
     CDS             complement(2856..4205)
                     /locus_tag="U3989_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1714853.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            complement(4180..4866)
                     /locus_tag="U3989_001398"
     CDS             complement(4180..4866)
                     /locus_tag="U3989_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1714854.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            complement(4985..5302)
                     /locus_tag="U3989_001399"
     CDS             complement(4985..5302)
                     /locus_tag="U3989_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN1714855.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            complement(5308..5739)
                     /locus_tag="U3989_001400"
     CDS             complement(5308..5739)
                     /locus_tag="U3989_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714856.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            complement(5996..6172)
                     /locus_tag="U3989_001401"
     CDS             complement(5996..6172)
                     /locus_tag="U3989_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714857.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            complement(6189..6668)
                     /locus_tag="U3989_001402"
     CDS             complement(6189..6668)
                     /locus_tag="U3989_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEN1714858.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            complement(6601..6918)
                     /locus_tag="U3989_001403"
     CDS             complement(6601..6918)
                     /locus_tag="U3989_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714859.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            7121..7591
                     /locus_tag="U3989_001404"
     CDS             7121..7591
                     /locus_tag="U3989_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714860.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(7634..7813)
                     /locus_tag="U3989_001405"
     CDS             complement(7634..7813)
                     /locus_tag="U3989_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714861.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(7800..8138)
                     /locus_tag="U3989_001406"
     CDS             complement(7800..8138)
                     /locus_tag="U3989_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN1714862.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(8154..8357)
                     /locus_tag="U3989_001407"
     CDS             complement(8154..8357)
                     /locus_tag="U3989_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1714863.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(8372..9148)
                     /locus_tag="U3989_001408"
     CDS             complement(8372..9148)
                     /locus_tag="U3989_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN1714864.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            9447..9767
                     /locus_tag="U3989_001409"
     CDS             9447..9767
                     /locus_tag="U3989_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1714865.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            9785..10216
                     /locus_tag="U3989_001410"
     CDS             9785..10216
                     /locus_tag="U3989_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1714866.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            10346..10807
                     /locus_tag="U3989_001411"
     CDS             10346..10807
                     /locus_tag="U3989_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN1714867.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            10841..11476
                     /locus_tag="U3989_001412"
     CDS             10841..11476
                     /locus_tag="U3989_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN1714868.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            11589..12224
                     /locus_tag="U3989_001413"
     CDS             11589..12224
                     /locus_tag="U3989_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714869.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            12523..>13258
                     /locus_tag="U3989_001414"
     CDS             12523..>13258
                     /locus_tag="U3989_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1714870.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETS"
BASE COUNT         3970 a         2573 c         2213 g         4502 t
ORIGIN      
        1 ccgtcgccag atgccgtcat cggtcccacg gatgatgggc ttgtggttcg ttgccagcca
       61 caacttgaat tcaggttcga actcgaattc ttttccataa aggtgccgag ccgtgacttt
      121 atcaccacca gtcaattgtt tgaccagacc ttcgtccagt cggacgcctt cattcggttc
      181 tgacgaagtg accaaccgcg cacctttcaa tcgtgcaata tcactgtttg ctgatgattg
      241 gctttgcttg accatgatcg tttgtgcctg catagtcatc gcatagtttc ccatcaattc
      301 agaaataatt tccaaaaata ccgactttcc atttgaccca tgaccgtaga ggatgaacat
      361 acattgctct ctggtagatc cggtcaggga atatccgaca catttctgga tatatctgat
      421 taattcttga tcattgtcaa aaatctgatt aataaattct tcccacatag gacaatcgac
      481 cgattctgtg tactcgacat ttgaaacacg tgtaaacatt ttgtctatct catgctcgtg
      541 tagaagaccg tcatgcaaag acagatagcc gctttgtgtg ttgaacagcg ttttatcttt
      601 gtcgaactct tccggcaata cggatagtcg atgtttcaat tcgtccatca tagcgttttt
      661 tccggcattg ctgcgagaac gcttgacgtg tttttctttc gctttcatca aagcttcttt
      721 ggtttcatcg tctgcgcctt cagggatcgt taacggttct tttcccatga tgtctactgt
      781 cgtatcgatc attttccgaa cttcgccggt attgtcctgt agccaaactt tcccatcgta
      841 gtaataccaa gatttatcaa tgtacgaata tttgactaac gttccgtaga tatcagtaaa
      901 ccgatccgcg ttgccggtgt cgtcgtacga atagtatttt tcctcttttg gctgttcctg
      961 gtctttgata aaaatcttaa aatcagattt ccgtttgggg ttgtagacgg aatttgtatc
     1021 agcaattgcc ttatttagta gagattctcc gtaagttgcc gatccccttt tttggtcata
     1081 tttgctacgg atcattgcag acgctctgaa aatctcatcc attttttcaa agtctctacc
     1141 ggtccaaaaa gccaagtcgt tggcgaacgc catatccgct tcggattgcg aggaataaaa
     1201 agcttcccaa ccgccgttca taaagacttt gaatcttgcc ccttgggaag acgattcagc
     1261 acgtttgata atttcttcaa ccggtaaatc aacggtattc atcagcggat tttcttcttt
     1321 gaattggatg attttttcat ctccaacgta gcgatcatat aggcgtttga tatcctttgg
     1381 attcggctca gttatttcta aatattttga tgcggcattt ccagtcataa cgaagaatcg
     1441 gccgttctgg tacatttcta cgtcaccttt cctgcgtctt ccgccgggca attctccgcg
     1501 agcaataatg tggatgccag tacctgattg agaatactca gcataggatc tcatgctttc
     1561 gataaattca tagataatat tttcttctat atcacctgat ttaaatcgct caacttcacc
     1621 ttcagcatta tcgatatcaa tgccgaaata aggtggtttg aaaaagaacc ccagtccact
     1681 cattccgtag tgatcaatag cagcgagagc ggtctgaaaa tcagaccagg tactctcgtc
     1741 attggatttg gctttatgcc cgttgttggc gttataaggg atcttcgtgt gcttctgccg
     1801 cttttcatcc caaactagct tataaacaca ccattgtttg agatttttaa gctcttttgg
     1861 aattttttca tacattgatt acacctcaat taaaatggaa gattgtcatt tctaaccgtt
     1921 gtcgctcttg aacgttccgg tccgaaggat ggttcatcac catttttgaa ttggtgattc
     1981 attacgcccc gtgtgttcgt tttatcccaa gacttcacat tgacgttttt atatccattt
     2041 gactcttcag ttttcacgcg tactgaggcg gttttcaaaa caaagtccgc cagcaattca
     2101 tcaaacccgt tatatgattt tccgtcttcc aactgtagcg cttgtgcaat tgccatgatc
     2161 ataccttcgt tatatttgcc ggtagctttg gctttccaga tttttgcgaa aatgtgttta
     2221 ttttggaacg gttgatccac atcgttacga acgatcaaat cgatatcgat aaattctgcg
     2281 cctgatttag ttgcattttc tacagctttg tttacgacca cttcgtacgt accgtctttg
     2341 atttcgttcc cttgttgcgc ttttgaaaag tctaagttaa atcctgtcat gttaatcatt
     2401 ctccttttta ttttcatatt tttctaagat aggtttgaaa tatttttctt ctgctgcaag
     2461 tcgtgcttgg attgcttctt ctttggtgtt gaaatccttt tgtaagacat attctccttt
     2521 gaaatataat cgtgctttcc atttatttct tcgtttatcc caattaacgc cttttgttcc
     2581 accgacagac aaagccttaa cgtaagttcc ttcaacatgg tatttttcat tagctttttt
     2641 taaattaacg tagccactct cttcgcctgt cccgttaaca acacctcttt ttatattaga
     2701 tagagaagat tcaaaaactt cgttattttt atttctagcg attacaattt gattgcctga
     2761 actatctctt tttccagtat cacctattat tgttacgcca tttattattg aaccttgaac
     2821 atcttctgct tttttaccaa aaataccgcc cattcctaca accacccctt cctcttagca
     2881 gccatataag cccaaccagg tttaaatcct ttagctcttg ctatggcata taagtcctct
     2941 atactttctg cttcatcttc cgtcatgttg taatatttat tgttctcaaa attcaaagta
     3001 attttcgttt ctccgacttt cattaattca gccgattcat cgatttctaa ctctgacttt
     3061 cgttcttcaa tcggctgcaa ctctccgcaa tacggacaaa tattatcgcc ttttggccgt
     3121 tcgtaagcac caaaacaaaa cgcgcattga acgatagata aatcactatc agtttttgtt
     3181 ttcttttttg tatctaaact ccattcacga tccatatcag gcaatccaaa acggttcaca
     3241 ttacccacat ggtcaatgat gattgacgtt ttatctggtc ggtaacgcat ccccctcatc
     3301 gattgctgaa tgtacaatga taacgattga gtaggtctca acataattac agtcgaacaa
     3361 tcaggaacat caaacccttc tccgatcaaa tcaacattgc ataggatttt aatttcatga
     3421 tttctgaacg cttcgataat atctgcacgt tcatcttttg gagtttttgc atcgatatgt
     3481 gttgctttat aaccagcatc gttgaatatt tcagcagtgt gtttacttgc atcgatactg
     3541 tgacagtatg ctattgcctg ttcaccttct gctaacgttt tataatgttt caacacgtcg
     3601 ccataaatag tattcttcac tgccttatcc attgatttct ttgtaaaatc tccagtcgaa
     3661 gctttcttta attcggctgt atcgatcaat ttaggtgcat aatattcata aggagctaat
     3721 ctatgattct caatcaacca tttagccgat actccttcaa ttagcaagtc gttcacatcc
     3781 cccaacccac taccgtttaa ccggatgggt gtcgcggtaa aacctaatcg cggaacgtcc
     3841 gaataatact catagatttt tctgtaggac gctgctaacc cgtgatggtt ttcgtcagtg
     3901 atgatcagtt gtggtttctt gatggtttta agatgtcgta cgactgtttg aaccatgccg
     3961 aaatttacaa gctgattatc tacgcccatc ttttgaaacg tatctttgat ctgatcaatc
     4021 aattcacgac gatggaccaa gaacaacact ctgttgcctt ttcgtgtggt tcgtttagcg
     4081 atctctgcaa ccatgactga ttttccagaa ccgcacggtg atacgataca tggtgccttg
     4141 taaccatcaa cataggcttg tcttgcacga tcgactaaat cattctggta gtcgtacaat
     4201 tccatctaaa atcagctcac tttgcttaca gccagatcta ttatccaatt gatttttggc
     4261 ataggttgaa tctgtggctt gtaaaataaa tccgcgttca tcgtcttcct ttaccatcaa
     4321 tcgaccaaca acgtcacata atcccaaaat attgtttcga atctttcgat tgatctgcgg
     4381 caaagcgatg ttgtaagaag atccatccga atccgtccac aaatcgattt cttcccatgc
     4441 ggtccagata aggttgctgt ctaaattttt catatagcgt aggctgttca ccattctaaa
     4501 ttgcatatat tgataatcgc cttgcgaagg aacgccgttg ttttttcctt ctgcgcctaa
     4561 actggacaga atacaccgtt ccaattcgct gacattatcg acaaccacgt tgtcataaac
     4621 acctacataa ttggtagtta aatcagccaa cgtgttgccc cagtcgttcc aggtatcttc
     4681 gttgtcgata tatacgatat cgacattttt ctcacctcta aggacttttg aagtccgatc
     4741 aatatcaagg atcagtgttt tccctggaaa atatttagcc gttgaagttt taccttttcc
     4801 tggagcaccg tagatcaaat atgttccctt ggtttgttcg atatccgtcg ccttcttaat
     4861 ctgcatcaaa attctccttt ctattggtta aaaaagtttt tcgattgtcg gtatgctgca
     4921 ttgttgatcc actatcttct tcaactgata tagtgattct tttaatcatt ttgtattcac
     4981 tctcctactc tggttcgtgc gcaataattt caacaccgac aatataatca cctagtctgt
     5041 cgaattcatc ttctacaagt gtactgtcgc ctttatcgtt agtaagttcc atagagaacc
     5101 aatcttcatc aaggtcacgg aaattaactg ggttatcctc gaaattagca tcatatagat
     5161 catctgaatc aatagctatt aatgataaac ccttagtgtt accgcccact ttgtaagtaa
     5221 catatccttt gtaaccccaa atcataaaag aaactttaat tgtgtgttta gcccatgaaa
     5281 tatttgggtc ataaatagtc aactctttca tttccccaac tcccctacct tttgatccgt
     5341 atactgccgt aactcgctca cgcgctgttc taactgctcc ttgtcgtttt gcactgtgga
     5401 tagttgttgg cgcaagctat cggcttcctg ttgctttgta gcgatctcct gttgcttttg
     5461 ttcgacttct cgttgcttgg cttcaatttc cttctgcttg tccgatttga tttgctcaat
     5521 ttcggctttc agctgctcct gtgtgcgagt gttgttggat agctgtgatt caagttctga
     5581 cacacgttgc gatttcgttt gaccgtattg taggactgtg ttgaaatttg ctttgatcgt
     5641 gtccaaatct tgaaacgcat tgcttgctgc gtagccgata acgccgctac ctagtgctag
     5701 tccgatgatt gctgttgttt ttgctagttt gtttttcaat gttgtttctc ctttttttgc
     5761 tttatagata atttagttca ttaaaaattc attcccacga ttaaaacgga agcctagaaa
     5821 ttgcaaacaa tgtaaatgca agagctactg ttatgctgta aattgcgtat aatttgtaag
     5881 actcagggcg aagcttcttc atgatatata tacaaaccgg tatgcacgac caaatgaatg
     5941 ttacgcagac tattacttgt aatacaacca ccctagccac tccttccgtg tggggttatt
     6001 tgtctaaata caattccgac aaaccaagca agtcggcgat aaaataaaga tgttctttcg
     6061 tcaaattttg aatgtcatgg acagttgctg gtttgccgtc aacctttatt cctttagctt
     6121 ccgcgatttc gatagcagct ttcaaagcgt cttttacgtt ttcgtgttcc attatttctc
     6181 ctccttcatc atcttatcct caaacctttt gtttggacga gttcagcacc aggaatatct
     6241 ccgtgcttca gttcctcctt caattgcttt ttatccaatt tgggaggcac aggggtaaag
     6301 aatccttttg gaattaagtt ctcattgatt acattgaccg atactggatt attttgaatt
     6361 ccaatgttga ataattcacc tttaatcttc gttttaccag tcttttccat ttcctcttgt
     6421 aaatagtgct tgatactctt agcattgttc aaaagcgatg tcttacgttc ctgcagacgt
     6481 ttaatttcac tatcgattaa ctcagctttc ccttctactt ctttaactat ttttgctaag
     6541 ttttctgcct tatactcgat cgcttcatta atcgaatcga gagtatcgcg aagaatttct
     6601 tcatccaatt gttctgccag ttccaggact ttgatatatg actcgctgag ttggtaaaga
     6661 gttgccattc ttttgtgcct cctttaataa ttttgcaatt tgttcaaaag ctaggattgc
     6721 ctcatctaaa tccaattcca cagaatcatc aatttgttcg aaagcaaggt ttgtttcttc
     6781 aatatcactt gcttgataga taccaatttt tccattgtca taaagatcaa atactaaaat
     6841 tcctgacgca tctatattgc gcagtttgta ttcgtctttt aaaaagatgc ggtctagtgt
     6901 atccgttgca attagcattt acggttcatt cctttctgtg gtaaaatata gaaagatagt
     6961 ttatttccct gacacgatca tgcttgccgg cgttcgtgtc tttttttatt tttcaaaatt
     7021 actaattctt gcattatttc ttctctcctt tttggtataa tttagttaaa aactggtggt
     7081 gtttattttg aatttttttc acttaacaat ttttcaatgg atgtcaataa taagttttat
     7141 attgagtacg tttttaacag ggataaaaat aaaaggaatg cgccctcaac tagaagttga
     7201 acttaatgct tcttacttcg ctgcagatat gatttataca aaagtaatca tttctaacta
     7261 ttcaacagaa ccagctatgt tagttaattt agaactttca tccgctaact taaaccgcag
     7321 atggtcagct actccattta agaaattaat tgctaaagga ggatcagtcg atgataatag
     7381 aatatactct gaagctgtac cgctaaatat tcctcctaaa agtgctatat cttgttacct
     7441 tgcatttgaa gtagggaaaa ttaattttaa taaactcctt aatcaccaaa caaaaatgat
     7501 atttactcta aatagaaccc aaatccacaa aattgttgat atcaaaaata caaacttccc
     7561 aatagagaaa ttagtgaaag aattaaactg agtaatagta atatacattc tagtttctct
     7621 tttctattca tttttagtca gccccctcgg ttggcttttt cgctctgtac tcagcttcag
     7681 cttcatcaag ccccataaaa atccaaacca tgtaaacaat cgttcctatc aacgcttgcc
     7741 tgcttcccca aagtcctaaa gcgtagatga ttagcggtgc gctgaacact agcgctctat
     7801 taaattttcc catccgcttg cctccttaag atttccgaaa aatttgtttc taaaaattca
     7861 agtgttttac ttcttaaaaa tagatatgtg tctcttcctt caactggata atagacaaat
     7921 ccatttttgt ttttctcgat atcgataata tttctatatc ttgggttctt taaaactcta
     7981 gaagtaaacc aatcatattt tctgttaatc cgttctagca cttctggcaa cgtcatccat
     8041 ctaccagtat catcagcttt ttttaactcc tcataatcca cttgggagat aattacatag
     8101 ccttctggaa ttgggatttt tgcttctaga tattgcatta gctgttcctc cctttacaat
     8161 tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt ctttttaccg
     8221 tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag catggtgtaa
     8281 gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg ttgagtgatt
     8341 tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa tccatttgag
     8401 ggtaatatcc ttcttttttt agtaattcgt agataaatag acgccctttc tgtgtccatt
     8461 tggtattcat tacaatttta gtgccaccat cggctttcgg gatctcagtt gtatgagatt
     8521 ttgtgtatcc ttggttcatg tgttttttgc acaataacca ttggttaccg acttttttct
     8581 gaatacctag tttatgaagt aatttattca tctgttgtgg agacatccca taatctgctg
     8641 caatctgact aattgttact gaatctgtag aagataatat gctatctaaa taggagattt
     8701 taggttcgta ctctgcaatc ttttgttctg cgattaatct tccagttctt tcttctttta
     8761 gttgagttgc taattgaatg attgtatctg gattaagcaa agcttcttct actttttctg
     8821 gagttagata acctccatgt tttctaattg ctggcaacac ttcacttgtt acccaacgtt
     8881 taaatttttt ggcagaggga agttttgatt ttaagattaa actgtacaaa ccagattcgt
     8941 tgatgattgt catctctctt gattgacctg aggtcgtgat tcgcgacccc atcttatctt
     9001 ccaaatctac atgccgcgac aaagcatctt tagtgtttga ataacccaaa acactcgcaa
     9061 cgtctttgcc tacaaaatat ggttcatcat ttactaaaat tgtccgaact tcgttttgtt
     9121 cgaaattaaa aatttgcggt gtgttcatat ttctcattcc tttcttttat attcgtaaac
     9181 aaatttaaca acttttttct aaattcaatt gacagtatct gagtaatact ctatactaag
     9241 agcataatta aataagacat aaaaacattg attttaaagc tttcttggcg gtctgcattt
     9301 acatatcaat agtgtttttt gttgtctttt tagttgttaa atctgtttac aagatatagt
     9361 atagctcatt actcagaatt agtcaaatta tttctgcgta tttttctaaa ctttttttgt
     9421 aaacatttag aaaggttgtt ttatcgatga atacctatga aataataaaa gacttggcga
     9481 agaggaaaaa gatatctata cggcagttag aaattaattt tggatattct aacggttatc
     9541 tagcaaagtg gaaaaataat actcctaatt cagatgaatt aagtcggatt gctgactatt
     9601 tcgatgtctc tgtagactac cttctaggta gagaagaaag agaaacccct aaacatgtgg
     9661 atttatcaga agacgatact gttttttctt ttgatggaaa agaaatatct aaggagacaa
     9721 tgcgtaaagc gattgcaatt gctaaagctt tagaggaaaa tgaatagttg gagtgatggt
     9781 ttgtatgtat ttgaaattga aagaaatgtt gagcgaatac aatttgaaag tagtttatat
     9841 ggagatgaaa gagcctggtt tctactatcc aaaacccaga atcatttttt tgaatgaaaa
     9901 tctatacggt gaaactgcag aagcctttca tttatctcat gaacttgcac atttcagcgc
     9961 ctctcatttt gaattttcag ttttgtacga tacttctaca acttttcact caaaatttga
    10021 aacagaagca gataaaatag ctattctaat tttattaaat atctatattg agaacgaact
    10081 gactgatgaa tctcagttta atctcgaaaa atttatggag tattattcta ttcagaataa
    10141 gctcagatac acttgttatg ctgtctgcca atgctatttt aagaaaaaat attcttacgc
    10201 aaggcaatat gtatgaatac atcaagaatt attaaaaaaa ccgatttcag caaaacatta
    10261 aaaaagcccg tgctgcaaca cgggctcttt cctcatatat gagcttctac aaaaaaaatc
    10321 atatcacaga aatgaggaag aaaaaatgga aaaagagatt ttaataaaat ctccaggaaa
    10381 aaccttgatt aaagtaactg atgattctat ttcaattatt agaaaaggat ttataaattt
    10441 agttaatcaa ggtattaaag gagaaaaaac tatccccttt aaaaatatat cagccgtaca
    10501 attaaaaaaa ccaggtatga gtaatggtta tatacaattt accttgctag gcggaaacga
    10561 aagtcgcgga ggtatattag cggccacgaa agatgaaaac accgttatgt ttacaaaaaa
    10621 atattggaat gaaatggaaa atttaaaaaa atatatagag aagcaacaat ctatattaga
    10681 taataattcc aaagaaacac aaatttcaag tgcagatgaa attaaaaagt ataaagagtt
    10741 gttagatgaa gggattatta atcaagaaga attcgatttt aagaaaaaag aattgcttgg
    10801 tttataatat tcaatatcaa aagagaaaga ggatctatta atggacggta tatttggttt
    10861 tactggtatt gttttattta ttataggttt aatcatgtta attgtaagat ttatcaaaaa
    10921 aacaaataag aaaacaccta tgattctcct tattatcggg attattttta ctaccgtagg
    10981 tttctcgctt tcctcatcaa acgaggcaaa tgacaataac aaacaggaaa gctcatcatc
    11041 tgatactcaa actactacat ctaacagcag caataaaagc ctaactcaat ttgaagaatt
    11101 tttagagaca aataatcatg actggggtgc tttcttagat agttattatt ctattactcc
    11161 agctactgag caaaatacag cttttactaa atatattagt ggtaaaacat atacttttga
    11221 aggaactgtt atcgaatcaa tgactactag aatagccatt atagcagaca aagagtacga
    11281 tgataaaagt tggaatgata tttctgcaac acctaaagtt tcatatgtaa tttttgcgaa
    11341 ggacttaaac aatgctgata cctttacaaa gggcgataaa gtaactttta caggagaaat
    11401 tagttcaaga ggatcgaaca tagaaaaatc ctatgctcaa tgggatatga ttaatagtaa
    11461 agtaagtaaa aaataaatga accacgcccc actaagagtt agattgtgca caataataaa
    11521 acctatacaa aagagtgcca tcaggagtca caatgcttgt aaaggaaatg acaaaatagg
    11581 aggtaactat ggataatagc aatgaaaaac taaccaaaaa agaatatctt gaattaatag
    11641 ttgaaggcgg tattggagca attccttata ttggtggcgt tgtgcagtca ctttattttg
    11701 ggtctaaaaa cgagaagcgt ttcaaacgca tcgagaaatt ctacaaaaat ttaaacgaat
    11761 cattagaaaa cattcgcgat caaattccag aagatgtttt caacttagag aacaaggacc
    11821 aactcattgg aatattcgaa gggataaatg acgaggtaga aaaatcaaag gcacaaaata
    11881 aaatagatat gtataaaaat ctttataaga attgtttact tcgaattaac aacgcttcat
    11941 gggataacga ggagtattat cttcttgtac tgaatcaatt aacatcgatt gagattcaat
    12001 tgttagcatg cttaatgagt cgtggaaacg aaaatttcac tggaaatatc agcatgtctg
    12061 gctattcaca agagctaata gatggtagct taaatagatt atctgatttt ggtctgttag
    12121 aaaaaacgat tagctccata gtattaggtg atgtcggcaa acaaaatatg ggctacaaaa
    12181 tatctcattt aggaattcaa tttattaact caactcttac ttaagttttc tttctaatat
    12241 gacatctaga cctatcagca atgcaacaat aaaaaaccag acataccaat aatcaacaaa
    12301 taatctaaac tctggaagtt cagttattct tttaaaaatc actttaatca taataaacac
    12361 ctctgattgg atgatctaat acacatccaa ttataccatt aaaacgacaa atagcacatc
    12421 cttgcgccgg caagcaaaaa ggatgtgcaa aaaataataa aacccaaaca atgggcttct
    12481 ttatagttcc tattgtatca gagaaagaga gctgattcaa ttatggcaag attagtcaaa
    12541 cgtggaaata gttggcaata cgaaatttca tacaaaaaag atgacggaaa atacacgaag
    12601 ataagaaaat caggatttaa gacaaaagga gaagcaaaag atgccgccaa cgaattagaa
    12661 tataacttga acaaaggcct taaaggggat cgcaaaaatc tattattatc agattacttt
    12721 gaggattgga tgcaacttta taaagaagga acagtatctc ctatcactta tagaaaatac
    12781 gaagatacgt taatgaacat aaagaaatat atgccagcgg tattgatttc tgatttagat
    12841 agagtgggat atcaacgctt tttaaataag tatgctaaag accatgtaaa atctaccgtt
    12901 attaagttta ataaccatat tagagcgtcg ttgaaagatg ccgtagaaga aggattaatt
    12961 ccgtttgatc caactagaaa agcagtaatc aaaggaaaag attcattgaa gccaaaagaa
    13021 gataaatatt tagattatga tcaatttaaa tctttaatga aactcgtaga agaaaacctt
    13081 tctgcgcagt actcttctcc tatgctcgtg ttagttgctg gtgccactgg aatgcgattt
    13141 gctgaacttc taggattaac atgggaagat atcgatttcg aagatcaaat catcactatt
    13201 aataaaacat ggaattataa attaaatgaa tggggaaaaa caaaaaacga aacttcaa
//
LOCUS       DASJON010000066        13235 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJON010000066 DASJON010000000
VERSION     DASJON010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.066"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            218..652
                     /locus_tag="U3989_001415"
     CDS             218..652
                     /locus_tag="U3989_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1714871.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            753..1844
                     /locus_tag="U3989_001416"
     CDS             753..1844
                     /locus_tag="U3989_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN1714872.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            1874..2551
                     /locus_tag="U3989_001417"
     CDS             1874..2551
                     /locus_tag="U3989_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN1714873.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2883..4178
                     /locus_tag="U3989_001418"
     CDS             2883..4178
                     /locus_tag="U3989_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1714874.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4168..5628
                     /locus_tag="U3989_001419"
     CDS             4168..5628
                     /locus_tag="U3989_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1714875.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5764..7260
                     /gene="glpK"
                     /locus_tag="U3989_001420"
     CDS             5764..7260
                     /gene="glpK"
                     /locus_tag="U3989_001420"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN1714876.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7274..9097
                     /gene="glpO"
                     /locus_tag="U3989_001421"
     CDS             7274..9097
                     /gene="glpO"
                     /locus_tag="U3989_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN1714877.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9098..9814
                     /locus_tag="U3989_001422"
     CDS             9098..9814
                     /locus_tag="U3989_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1714878.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10120..12049
                     /locus_tag="U3989_001423"
                     /pseudo
     CDS             10120..12049
                     /locus_tag="U3989_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            12175..12729
                     /locus_tag="U3989_001424"
     CDS             12175..12729
                     /locus_tag="U3989_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN1714879.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4423 a         2216 c         2964 g         3632 t
ORIGIN      
        1 ttcacaaaat ttttcttcat actcaaaact aaacgacttt ttcacaacct ctgattcacg
       61 gtgttccaga aggagttttg tcgaaaacaa gccgaaattc tttgaagatg gctacttctt
      121 tttttcaaat ctagacagag gaggcggaca ttattccgtg ttttttgaca aaaaataaaa
      181 atcatactat gatatgtacg ccaatggagg ggatgaaatg gaaaagccaa ttgaaaaagc
      241 gttagatgaa tgtcttttct ttagcgtgaa aaagctagat cgcatgttga ataaattagc
      301 tgatgaagcg tttagaagga caggtctggc accaacttat ggttttattt tattgatatt
      361 aaaagaaaaa gatggaatac cacaaaaaga tattgcacaa atgctctatt ccgccccatc
      421 aacgattgct cgttttgttg aaaaattaga atataaagga tacgtcaaaa cagttagcga
      481 aggtcgcttg tcattagttt atcttacaga acaaggacgc caatttgcta aggaagtcga
      541 tggtagctgg gaagaacttc atcagtcata caaagcagtt ttgggaaatg aagcaagtaa
      601 agaattagcc cagacaatga atgatgcaac agataagtta caaaaaaaat aaaatttttt
      661 aagcaaatta gttgcatgta caactaatcg agtggtttaa tgatagatga ttctcgatag
      721 gttaatcaga gcgaattatg ggaggaatga caatgtcaca atttgatcaa tcaattactt
      781 taccttcagg agttcaattg aagaaccgat tgatgctgtc gcctatgacg acacaactca
      841 gctttttcaa tggagtgatc accgaagatg agatcactta ttataaacaa cgttctaaag
      901 gtcttggtgc tgttattact ggtgctgcaa atgtccaaga tatcggaaaa ggctggccag
      961 gtgaactcag catcgctcat gatgaaatga ttcctcgatt aagtgagcta gcgaaagcaa
     1021 tccaaggaga aggcgcaaaa gcgattattc agattttcca cggtggacga atgacaagtc
     1081 gagcaacttt atctggagaa cagcctgtat ctgcaagtgc agttgcagca aaacgtcctg
     1141 atgcggaaac accacgagca atgagtgaag aagaaatcgt tgaaacaatc caagcatttg
     1201 gcaaagccac acgtagagcg attgaatcag gatttgatgg aatcgagttg catggagcaa
     1261 atacgtattt gatccaacaa tttttctctc cgcattcgaa tagacgagag gatcaatggg
     1321 gcggtagctt ggaaaatcgt tatcgtttta ttgaagaatt attaacaacg gttttctcag
     1381 cggttgaaca atatgctaaa aaaccattca tcgtagggta tcgtttctca ccagaagaat
     1441 atgaaacacc aggtatccgt tttgaagaca cgatctggct tcttgaacga ttacgtgagt
     1501 caaaactaga ttatctgcat gtttcattaa atatgtatga tcgaatagct cgttctgata
     1561 aatacaacga taaatcgatt cttgaagtgg tccataacac gattcaagga aagatccctt
     1621 tagttggtgt cggtgggatc cgcaatcgcg aagatgtaga gcaggtattg aaatatgcag
     1681 aacttgcagc aattggacag caaatgatcg tcgatcctga ctgggcagag aaattattgg
     1741 aaaaccgtga ggatgaattt gtaacgaaac cttttgaaaa agcatataaa gagctttatt
     1801 tgccaagccc attatataat ttcttgaaag cacgttatca ataacgcaat ttttatagaa
     1861 agaggatggg aaaatgaaag tattgatcgt attaacaaat gtagaaaagt acgaaaagat
     1921 cgagcgtcct acaggtttgt ggttaagtga actgactcat ttttatgatg tattagtaaa
     1981 aaatgggatc gatgcagatt ttgtcagtcc taaaggcgga tatgtacctt tagaaccgca
     2041 aagtgttttg aatatggatg aaatcgattg ggcctattat gaagatgaag atttcagaaa
     2101 tcgggccttg ggaaatacct tacgtcctga tgatgtggat gcaaatgatt atgcagcggt
     2161 ttattatgct ggtggccacg gaacaatgtg ggattttcca gaagcaatag atcttgggaa
     2221 agtcgcttca caaatctatc gaaatgaagg gctggtctca gctgtttgtc acggtgttgt
     2281 aggattgttg ccagttacag atgagtctgg aaaatctatt ttagctggac gtacagtgac
     2341 tgggttcagt aatgaagaag aggaagcaaa tggtacaacg aatgaagtac cattcttagc
     2401 agaagatgct ttgaaagaaa aaggagcaaa ctatcagtct ggagcagctt tttcagaagt
     2461 ggttcaaatt gatgggcggc ttcttacagg gcaaaatcct caatctgctc atcaattagg
     2521 cgaagcagtt gttgaagcat taaagaatta aaaataaatg atgtgtctat tctaactatt
     2581 acattacaca cgctaaaact cgtcttttcg ttcttttctg tttcctattt ggacagaggt
     2641 acgtaaaagg cgagtttttt tcttttataa gagtacattt tttgaaacat ctcttcagaa
     2701 atgatactct tcaattaggt ccactagtac attattgaga agcagaccta gaacacggta
     2761 tgatggagag ggttgattct gctgcatcgc tatgtatcat aaggttgaaa gcgagttatg
     2821 aatactgtgt tttctgattg agaagttagc agtagaacac cttataagaa agaggaatgc
     2881 agatgaacat tgtaattgtc ggcgggtcgt ttgcagggat tcatgcggct atttatcttc
     2941 gccaattgat gccaacaagt gaaatatatt tgattgaaaa gcaatcgaag ttaggatgga
     3001 ttcccagcgg gttcaatttg atattaaaag gaaaggttac cagtgaaaaa caactttctt
     3061 ggatcacgaa agaagaactg actgaccgct atcgaatcca tgtttacaca gaaaagacag
     3121 taataggatt gaaagaaaaa aaagtcgttt tagcaggagg aaaaaaattg gattttgatc
     3181 gtttgattct agcgactggt tcaaatcaaa agtttcgaaa tatttctgca gaatcaacgc
     3241 ttattcaccc agttaaaaag atacaaaacg taacggctct tcagcaaaag atcatgaatg
     3301 caaagaaaat tgcaattatt ggtgcaggac aagcaggaat cgaaatagct gaagggttgg
     3361 catcaagaaa aaaacaaatc catctttacg aaagccgaaa gtcaattctt tttcgttatc
     3421 tagatcctga aatgacagaa ccgttagtaa aggaaatgaa aaatcaaggg ctttcacttt
     3481 ttttagaaga acaagttgtg tctttgaccg aaaatgaaac cgctattgta gagacggaaa
     3541 aaagaaagga gaactatgat cttgttttgc tggctaatca ttctcgtccc gacaatcaga
     3601 tgtgggaaga acaattaaca ttgaatgatg atggaacgat ctgggtagat gactacttgc
     3661 aaacgtctca aaaagacgtt tatgcgattg gcgatgcgat ccaagtaact tttcgtccaa
     3721 caaatgaaaa aatgtatgtt tctctagtaa ataacgctgt ccgtacagca cgaaatgtca
     3781 gcaagacaat tagtggctca cagacaaagg atcagggaac ctatcgtcca gtcggcaatc
     3841 agtggtttgg ctatttctta ggaagcgtag gtttgacaga agaagagagc attttttatc
     3901 cccaaaaaat tgaaacagga tacgtagact acaagcttac agcaataaat caaagcaaag
     3961 ttaaactcaa atttctattt gattctgaaa aaaagattct tggagcacag ctccaaagca
     4021 aaaaagagat gttccacctt cttgaccaac tgacaattgc agtagaagaa ggatggacgt
     4081 ttgaacagtt agaagaacat gagctatttt tccagccaga ataccgtgtg ccagatatgg
     4141 tgtggtcaaa ggtggcgaat actgcctatg aagattgaag cattattaga aagaaaagag
     4201 cagttacaga tcctgatttt gcgaaacctt gttctgcaag ggggaacagc ctctatcaat
     4261 ggtttgagag agcatgtcca actgtcaaaa gcctcgtttg atcaatattt ggaagatatt
     4321 gaattgattg gccgaatgat ggaaaaaaaa gtagaagtac agcgaaatga gtatcaggcg
     4381 ctgcttgtgt tggacgaaga tgtaagtttg gaaaaaattc tattgttttt attgcaggaa
     4441 agcttgaagt ttaagatgct cgtctattta ttagaacatc agcaagtatc catcgttcga
     4501 ttagcgacag cgtttaatat cagtgagtct tctgtttttc gaaagatcaa agaattgaac
     4561 cagctcttag aggaatttgg attacaaatc aaaaacggtc aattatacgg agaagagcta
     4621 cagatacgat atttttatta tgaactattc caatatatcc cggaagatca gcgaccgctt
     4681 tttttgcaaa atacaccaga aaaaagaccg ttcattctag gattggaccg tgtattagaa
     4741 acaacgttta cggcatcagc agaagcacag attgcttgct ggctcgggat tacgaaaaaa
     4801 agactattga atgaaaaaag tacgtacgca actttgaaag aaaagaaatt actctatcag
     4861 tcggaccggc tttatcaagc aatcgatcca atcatcgtga tgcatcttag tcgaacagca
     4921 gcagaactga atgtatacga aacaatgatg ttttacagct tttttgtttc tttttcgata
     4981 gtagacgaag aaatatttta tcagtatgat ttgacacgga gcaaaaagtt accaaccgct
     5041 gttttagata cttacattcg ggaaacgatg ttatggcatt atcgtcctcg tcgtttgaaa
     5101 atcaaagaag aaaaggcagt aggctaccaa atttcccaga tcaacaatga atggtttttc
     5161 tttgttggaa agatcgaagt ctatgagcgt gatcggcttt tggaacagca gcagaaaatg
     5221 ctcgggcatt ccttaacaca gttactggcg aaattacagg aaacagcggt gcagcaattg
     5281 ccgaggaaaa gagctgaaga tagtgagctt agttacctga tgatccagta tgccaatgtt
     5341 cttttgatga tcgattttta tattgcaaaa ccagtagtga ttggaatcga tttagaaagt
     5401 ctgccgattt atcgtattgc ttttcaacaa tatctgatca gagaactgag gggaatcggc
     5461 ggtattgaga ttggaagtta tgaagaaggt aaagaatatg atttggtgat cacattttgc
     5521 cagagaaaca aaaaacagtg tgaatattat ctgtcagaat ttgcgtcacc ttacgacatt
     5581 attcgattga aaagaagaat agagacgctg aaaaaagaaa agaattgatt aaaaaatagc
     5641 ttcataataa aaaactgtca aaaaaagaaa aaaagaataa aattttgaca gtttcttttt
     5701 ttaaaacagt agtatgataa gagtagttaa agaaagcgtt tgcatataat ggagggaatc
     5761 acaatgacag aatcaacgta tatcatggcg attgaccaag gtacaactag ctcacgagca
     5821 attatctttg acaagaaagg aagacacatc ggcagttctc aaaaagagtt cacgcaatat
     5881 ttcccgcaag aaagttgggt ggaacatgat gcaaatgaaa tctggaattc tgtacagtca
     5941 gttattgccg gagcgttcat cgaatcaggg atcaaaccaa atcagattgc tgggattggc
     6001 ataacgaacc agcgtgaaac aacagtaata tgggaaaaag atacaggacg tccaatctat
     6061 catgcagttg tttggcaatc cagacagtca gctggaattg cagataactt aaaaaatgaa
     6121 ggctatcaag aatttttcca cgaaaaaaca gggttagtga tcgatgccta tttctcagca
     6181 acgaagatac gctggattct tgatcatgta gaaggcgcgc aagaacgagc tgaaaaaggc
     6241 gaattgatgt ttggaacgat cgattcttgg ttagtatgga aactgaccga tggacaagca
     6301 catgttactg attactcgaa tgccagtcgg acgatgttgt tcaatatcca tcagctagat
     6361 tgggatcagg agattttaga tttactaaat attcctcgag tgatgttacc aaaagtcact
     6421 tctaactcag aagtttatgg ttatacgcaa ggttatcatt tttatggaag cgaagcacca
     6481 atttcaggga tggcaggaga ccagcaagcc gcattattcg gacaaatggc attcgaacca
     6541 ggcatggtca agaatactta tggtacaggt tcatttattg tcatgaatac cggtgaaaaa
     6601 ccgcaattat ccaaaaataa tttattgacg acaattggtt acggcataaa tggaaaggtt
     6661 tactatgctt tagaaggaag tatttttgtg gctggttctt cgattcaatg gctgagagat
     6721 ggacttcaaa tgttgcaaaa agctagcgat tcagaagacg cagcgaaacg ctctacaagc
     6781 gaagatgagg tttatgttgt tcctgcattc gttgggctag gtgctcctta ttgggatcag
     6841 gcagcacgag gggctatgtt tggcttgaca cgtggtacga caaaagaaga tatcattaaa
     6901 gctactttac agtcgattgc ttatcaagtg agagatatca tcgatacgat gcaagatgac
     6961 actggaatca agattcctgt attgaaagta gatggcggtg cagcaaacaa tgaatatctg
     7021 atgcaattcc aaacagatat cttgaatgta ccgatccaac gagcagaaaa cttggaaaca
     7081 acggcgctag gggcagcatt tttagcagga ttagctgtcg gttattggaa agatacagac
     7141 gagatccgcg aattttatga agctggcaag ctatttgaag ttcaaatgga ggaagagcgc
     7201 cgagaaaaac tttacaacgg ttggaaaaaa gcagtaaaag ctacccaagc atttgaataa
     7261 ggaggaaatc catatgtttt caaacaagac aagacaagat agcattcaaa aaatgcagca
     7321 agaagaattg gatctgttga tcatcggtgg cggaatcact ggtgccggtg tggcagtcca
     7381 gtcagcagca tcaggaatca aaacaggatt gatcgaaatg caggattttg cagaagggac
     7441 ttcctctcgc tcgaccaaac ttgttcatgg cggtattcgt tatctgaaga catttgatgt
     7501 ggaagtagta gctgacacag ttggtgaacg tgcagtcgta caaagtattg ccccacacat
     7561 cccaaaacca gatccaatgc ttttgccaat ctatgagggc gaaggagcaa caaccttcaa
     7621 tatgttctcg gtcaaagtag caatggacct ttatgacaaa ctggcaaatg tgacaggaac
     7681 caaatatgag aattatacgc tgacaccaga agaagtattg gaaagagaac cgtttttgaa
     7741 aaaagaaggg ctaaaaggcg cgggtgtcta tctagatttc cgcaacaatg atgcccgttt
     7801 agtgattgat aatatcaaaa aggctgccga agatggggct tatctagtaa gtaaaatgaa
     7861 agcggttggc tttttatatg atggcgatca aatcgttggc gtcaaagctc gtgacctgct
     7921 gacagatgaa gtgatcgaaa tcaaagcaaa attagtgatc aatacgagtg gtccttgggt
     7981 agataaagta cgaaacttga attttacgcg tccagtctct cctaaaatgc gtccaactaa
     8041 agggatccat ttagttgtag atgcgaaaaa actgcctgta ccgcaaccca catacttcga
     8101 tacaggaaag caagatgggc ggatggtttt tgctattcca agagaaaaca agacttactt
     8161 tggtacgaca gatacggatt accaaggaga ttttacggat ccgaaagtca cacaagaaga
     8221 tgtagattat ctattggatg tgatcaatca tcgctatcca gaagcaaata tcacattgaa
     8281 tgatatcgaa gcaagctggg caggacttcg tccactgttg attggtaatt ctggttctga
     8341 ttacaatggt ggagataatg gatcgatttc agacaagagc ttcaataaag ttgtcgatac
     8401 agtaagtgaa tataaggaaa acaaagtttc tcgtgctgaa gtagaagacg tgttgaacca
     8461 tttggaaaac agccgtgatg aaaaagcacc ttctacgatt tccagaggta gttctttaga
     8521 aagagaacca gatggtttgc tgactttatc tggtgggaaa atcaccgatt accgtaagat
     8581 ggcagaagga gctttgcgat tgatacgcca attattaaaa gaagaatacg gaatagagac
     8641 gaaagaaatc gactctaaaa aatatcagat ttcaggtgga aacttcgatc caactaaatt
     8701 agaagaaaca gtgacagaat tagcaaaaga aggagtagca gccggtttag aggaagaaga
     8761 tgctacttat atcgctgatt tttacgggac taatgcccga cgtatctttg aattagcaaa
     8821 agaaatggca ccttatcctg gtttgagtct cgctgagtca gctcggttac gttatggttt
     8881 agaagaagaa atggttttgg ccccaggcga ttatctcatt cgtcgtacaa atcatctgtt
     8941 gtttgaacga gatcagcttg atgagatcaa gcaacctgtg atcgatgcaa ttgctggata
     9001 ttttgaatgg acagaagagg agaaggcgca acagactaaa cgtttagaag cattgattgc
     9061 agaatcagat ctgcgggaac taaaggggga gaaataaatg aacagtgata tgactcaaat
     9121 catgggggag tttattggga cactgatcct agttttgctt ggagatggtg tgtgtgcggc
     9181 tgtgaattta aacaaaagta aagcacaggc ttctggctgg attgtcatcg catttggttg
     9241 gggactcgct gtaacaatgg ctgtctatat ttctggattc atgggcccag ctcatttgaa
     9301 tcctgcagtc tctctagcta tggcgatgac aggggcaatt agttggaatc ttgtcgttcc
     9361 tttcatcatt gcgcaagttt taggagcttt tgcaggggcc attcttgttt ggctttctta
     9421 tttgccacat tggaatgcaa cgaaagatga aagcgcgatt ttaggaacgt ttgcaacagg
     9481 tcctgctata cggaattatc cagcaaatgt gatcacagaa ttaattggta catttgtatt
     9541 agttcttggg ttgctagctt ttggacaaaa cgaatttgca cctggaacga acgtattcgc
     9601 tgtaggtgga ttgatcttag ccatcggtct ttcattagga gggccaacag gatatgcaat
     9661 caaccctgct cgggactttg gtcctcgatt agcacatgcg gtattgccaa tagctaataa
     9721 aggaacttca gattgggcat attcatgggt accaatcgca ggtccaatga tcggagcaat
     9781 tatcgcggta ggtgtctatt cattaatggt gtaaatctat aaaacatact aaagtttgac
     9841 aaccaaaatg tgtcttgcta tagttaagtg gaaaataaat aagacctaga agaatggggt
     9901 atgttttgtg agtgtagctg gtatctaatc agtaagtcga atagccaata aacaaagttt
     9961 atttggtgtg cttatgaaac ggtacctgtc aggaaaacaa tatctttgta ttctaataag
    10021 gataggacat gactttttta agctgtgttc tatcctttta tttatctcta ggcccttatt
    10081 tattttggga caggtatctc ttcaagaaaa ggagagaata tggacaacga aatgattagt
    10141 caattacaat ttgatcggat caaaaaggaa atccaagcaa gagcaattgg aaactatagc
    10201 aaaaaacgaa tcagcgatat gacggtctcg acaaacctac aaactgtctt ggatcgtcaa
    10261 gaagaaacaa gagaagcacg cttgatctta gaaagcagtc agcatgttcc atttatgggg
    10321 ttgccgagga tcgatgcact gacagaacaa gtcaaaaaag ggttgattct gcagccaacc
    10381 gatttgatcg aatatgcgga ttttcttaga agcagtcgga tgatcacaaa gttttttgat
    10441 aagaatcaat accagacacc cttgttgttt gcttatagca aacatcttcc tgatttgata
    10501 aatgtagaag agttgattga ccagaaaata aaaaataata aagtcagtga cgacgcgtca
    10561 cgtaatttgc gtaaagtacg caaacaatta caactaatcg aaaaagaaat ccagtcaaaa
    10621 ttactgaaat tcttgcgtca tccgaaaaac aaagaaatga tccaagaagc aatgattgtt
    10681 caaaaaggcg aatactatac gattcctatc aaagccagct ataaaaataa aatagatgga
    10741 acgattatcg atgaatcaaa taaaagaacg acggttttta ttgaaccaac tgtgatttct
    10801 aaattgaatg aacactatca gttgttaaaa gctgaagaaa tcagtgaaga atatcaagtc
    10861 ctggcggcat tgactggtgc tatcgcagaa aacgaagaag tgatcgattt attgatcgaa
    10921 acgatcacgg tgttagatat catctttgct cgggcaaaat ttagccgaga aatcaatgga
    10981 agcacaccta gaatcaataa atcggaacat atcattatca agcaaggcag acatccattt
    11041 ttacctgagc atgcggtacc attggatgtt gaaatcggta aagactatcg aggcttgatt
    11101 atcaccggag caaatgccgg aggcaaaaca gtcgtgttga agacagtcgg tttattgact
    11161 ttgatggcga tgttcggtat gcaagttccg gcaaaagaag ggacagagct tgctgtcttt
    11221 gatgagattt tcgtcgatgt cggtgaccac caaaatttag aaaatgcgtt gagtacattt
    11281 tctggtcata tgcaaaacat cgctgctatt ttgaaaaaga taaaaaggaa tacgttggta
    11341 cttttagatg agattggcag tggaacagag ccaaatgaag gggctgcact agcgatcgca
    11401 atcatggaat ctatgtatga gcaaggggca ttgatcattg ccacgaccca ttatggagaa
    11461 atcaagaaat tcgccggaga tcatgaagat tttgtgccgg ctgctatggc tttcgatcgg
    11521 gaagcacttc gtcctaaata tcagttgcgg gttggaagaa acaggagaaa gccaagcttt
    11581 gtggattgct cataaaatgg atatgtcgat gaaattgatc catcaagcag agcgttactt
    11641 ggcggaaaaa gcctatcgga cacagaaaaa agaatttctt cctaagacag cagttacaaa
    11701 taggaaggag aataaaaaag aacgtcagct ctttgcaaaa ggtgatcgga tatttgtcaa
    11761 tgaataccaa aaagaagcgt tagtttatga agatataggt gaagatacga ttgatgtata
    11821 tttagacaaa aaaataatcc acgtgcctcg tcagcgtgtt cgtctggtgc gttcagcaga
    11881 agatctgtac ccgacaggtt acgatttaga cagtctgttt atcgattata aaacacgtaa
    11941 acggcagcgt gacctagagc gaggatcaaa aaaagctcat aaagtactag taaaagaaat
    12001 gcgcaaacga caggaagaaa gaagagtaaa cgatgaaaac agcaagtgat tatctcaaat
    12061 tagctcataa tatcagtgtc tgtaaagaaa aataaaaaca gtgaaatcat tggttttatt
    12121 ccccgtgtaa gctaaaataa aaagaaaaga gtgctgagga ggtagggcga taaaatgaaa
    12181 ttaagcacag actggcgtaa agaaatacaa acgattccga atcttttatc gatattccgt
    12241 atttttttgc tgccgattta tctttatttt gtgctgagac agtcatttta tatagcagga
    12301 gcggtcattg ttgtctcagg tttgtctgat tatctagacg gggtcattgc tcggagatat
    12361 aatcaggtaa cagacttagg gaaggtgctt gatccgtttg cagataaatt gacacaactt
    12421 tttctgattt tgtctatggc atggtatcga ccgtggcttt ggctgttgtt tggtctattc
    12481 ttgataaaag aaggctttat gtttgtagct ggattaatcg ggctatctaa aaatatcaaa
    12541 ttaagtggag cgaagtggta tggcaaagta gcgactgctg tcatctatgt tggcatgatc
    12601 cttttgctgc ttttcccaga attaccaacg ctgtgggtga gagtcatttt tgccgtcatc
    12661 acttacggtc ttttacaatc ttttgtcttg tatgctgtcg agtaccgaaa gatgtttcaa
    12721 agaaaataaa aaaggggtgt gacctatgtc gcattccttt ttttgataaa tagactccca
    12781 gtagctactt ctcgataata agccaaccga accaaaaaca ggagaagttg tttttgaaat
    12841 tatttcttgt gattcggagt taaatggtag gctcacaacc tcttggtaaa cggtgttcaa
    12901 tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgagcagcta tttttccgtt
    12961 tgtttctcta atactcaggt caaaccgctg attttacaac ctcttcctta aacggtattc
    13021 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaat ccattttcac
    13081 aaaatttttc ttaatactcg aaactaagcg actttttcac aatctcttcc ttaaacggtg
    13141 ttcaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttc
    13201 ccgttttttt ctctaatact taggtcaaac cgctg
//
LOCUS       DASJON010000067        13096 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJON010000067 DASJON010000000
VERSION     DASJON010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.067"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2009)
                     /gene="pnp"
                     /locus_tag="U3989_001425"
     CDS             complement(<1..2009)
                     /gene="pnp"
                     /locus_tag="U3989_001425"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN1714880.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVV"
     gene            complement(2197..2466)
                     /gene="rpsO"
                     /locus_tag="U3989_001426"
     CDS             complement(2197..2466)
                     /gene="rpsO"
                     /locus_tag="U3989_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN1714881.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2717..3280
                     /locus_tag="U3989_001427"
     CDS             2717..3280
                     /locus_tag="U3989_001427"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1714882.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3533..4054)
                     /locus_tag="U3989_001428"
     CDS             complement(3533..4054)
                     /locus_tag="U3989_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN1714883.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4057..4899)
                     /locus_tag="U3989_001429"
     CDS             complement(4057..4899)
                     /locus_tag="U3989_001429"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN1714884.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(4892..5617)
                     /locus_tag="U3989_001430"
     CDS             complement(4892..5617)
                     /locus_tag="U3989_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN1714885.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            5793..6089
                     /locus_tag="U3989_001431"
     CDS             5793..6089
                     /locus_tag="U3989_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714886.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            6657..8249
                     /locus_tag="U3989_001432"
     CDS             6657..8249
                     /locus_tag="U3989_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1714887.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8262..9002
                     /locus_tag="U3989_001433"
     CDS             8262..9002
                     /locus_tag="U3989_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1714888.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9170..10519
                     /locus_tag="U3989_001434"
     CDS             9170..10519
                     /locus_tag="U3989_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1714889.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(10647..11237)
                     /locus_tag="U3989_001435"
     CDS             complement(10647..11237)
                     /locus_tag="U3989_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1714890.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            11384..12736
                     /gene="brnQ"
                     /locus_tag="U3989_001436"
     CDS             11384..12736
                     /gene="brnQ"
                     /locus_tag="U3989_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1714891.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         3682 a         2575 c         2596 g         4243 t
ORIGIN      
        1 acaacatctt cgactttatt gacacgatcg tttgctaatt gggaaatatg gacaagtcca
       61 tcttttcctt tgatcaagtt gacgaatgca ccgaattttt cgatacggac aactttccct
      121 agataaactt caccaacttt gacttctttg gtcaaatctt cgatgatctt gatagctttt
      181 ttgatcattt ctgcatcagc tgaagcaata cttacttttc catcttgatc gatatcgatt
      241 ttcacaccag tttcatcgat gattccgttg atcgtatcgc cgcctttacc gataacatct
      301 ttgattttag caggttcgat ctggatcatt tcgatttttg gtgcatattg gcttaattct
      361 tcacgtggag cagcaatcgt tgaagtcaat tcttctaaga tttccatacg tgcttgtttt
      421 gcttgagtta atgcttcagt taggatttgt tctgtgattc cttgaatctt gatatccatc
      481 tgtaaagcag tgataccatc ttttgtaccg gcaactttaa agtccatatc gccaagatga
      541 tcttccagcc cttggatatc agttaggatc gtatagtttt caccatcaga aacaagtccc
      601 atcgcaatac cggcgactgg cgctttgatt ggtacacctg catccatcaa tgctaatgtg
      661 ccagcacaga tacttgcttg ggaagaagaa ccatttgatt ccaatacttc tgctactaaa
      721 cggatcgtat aaggaaattc ttcttctgaa gggattactt gtgctaacgc acgttcacct
      781 aatgcgccat gtccgatttc ccgacgccct ggagagcctg cacgtcctgt agaaccaaca
      841 gaaaattgag ggaaattgta gtggtggatg aatcgtttgc tgacttccac ccctaaacca
      901 tcaatgattt ggtgctctcc tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt
      961 cctcgtgtga acaaacctga tccatgtaca cgtggcaata agctgacctc tgatgacaat
     1021 ggacggattt catccaattt acgcccgtca ggacggatct tatcgatggt gatcaattca
     1081 cgtacgacat ctttttcaag atcttctgtg atttgtttaa cttctttcaa caagtgatct
     1141 tcatcttcat gacctgcaaa tttctcagca taagcttcgc ggatatcgat cttcacctgc
     1201 tcgatattat cttcgcgagc aagtttttct tcagtcatta ctgctgcttt cattgtagcg
     1261 taagaagcat caaagatttc ttttttcaat acttcatcaa cttgcagtaa agtcacttcc
     1321 attttttctt tccctactgc ttgaacgatt tcttcttgga aagcaactaa ctctttgata
     1381 gcgtcaaaac caaacagcaa tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg
     1441 ctttcaacca tattgatggc ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt
     1501 tctgcttgtt ctactgttgg attcaagacg tactcgccat ttacacgacc aacttctacg
     1561 cctgcgattg ggccgtcaaa tgggatatct gagattgata gtgctaaaga agaacctagc
     1621 attgctgcca tagctggcga gcagtctgtt tctacactca tcacgatgtt cgttacttgg
     1681 acttcattac ggaagccttc tgcaaacatc gggcgaattg gacggtcgat caaacgggca
     1741 gtcaatgttg cttccgtact tggacggcct tctcgtttga tgaaacctcc agggattttc
     1801 cctgctgcat acattttttc ttcataattg atcgttaatg ggaaaaagtc tgtatctttt
     1861 gcttctttag aggccacagc tgcacttaag actactgtat ctccataacg gactaaaacg
     1921 gctccgtttg cttgttttgc tagctgaccg acttcgattt gtaagggacg gccgccccat
     1981 gtcgttttga atacttgttt ttctgacata acttctcctt ttccttctcg tgaaagcaag
     2041 acgctactaa acaaatgaaa actgccgaaa gccgtctttt gagcagcttt catttggtta
     2101 gtaggagtcc atctagcttg atcacggaat tttacttaat tgatatacga gtaaaaaaag
     2161 tgagattcat gtgaatccca ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt
     2221 gatcaattcg cggtaacgtt gaacgtcagt tttacgtaag taagctaaca agttacggcg
     2281 gtgaccaact tttttcatta ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc
     2341 atgttcattc aagtggttga tttcttcagt taatacagcg atttgtactt ctggagaacc
     2401 agtatcccct tcatggcgag cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat
     2461 tgccatttct ttcacctctt tcgattattc ccctgcgact gagtaaaacg ttggtgattc
     2521 gcttaaccaa gtggcaggtc atgtgcctag agcacacagc tatactttac ctgaaaccgg
     2581 gttgaaaatc aagtagttaa tcattaagaa aaggtagttt tcaaacaatc ttactactac
     2641 ttttccattt ttgtggtaaa ctataatgcg aaaatagact tgaaacacag tcttttaaga
     2701 aaggggtaca tattccatga ttacaatgga tgatatcatt cgtgaaggaa atccgacctt
     2761 acgcgaagtt gcaaaagaag tttctcttcc attaagcgaa gaagatatct ctttaggaaa
     2821 agagatgctt gagttcttaa aaaacagcca agatccaatc aaagctgaag aactacactt
     2881 acgaggcggt gttggcttag ctgctcctca attagatatt tctaaaagaa tcattgctgt
     2941 tcacgtacca agtcctgacc cagaggcaga tggaccttct ctaagtactg ttatgtataa
     3001 tcctaaaatt ttgagccatt ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt
     3061 atctgttgat cgagaagtac caggatatgt tgttcgccat gccaaaatca ctgtgtctta
     3121 ttatgatatg aatggtgaaa aacacaagat tcgtttgaag aattatgaat cgatcgtcgt
     3181 tcaacatgaa atcgaccata tcaacggtgt gatgttctat gaccacatca acgatcaaaa
     3241 tccatttgca ctaaaagaag gcgtattggt gatcgagtag agaattggca ggttccttcg
     3301 atactatgct acaagaaagc tttataaaaa aggggagtgc tcaaaagctg cacttccctt
     3361 ttttatgttt ctccactaat cgaaatttag atagacatct agaaatcgca attccaaaaa
     3421 attctgtgta ttggatacgc atcttttctt attttttctt atttcttcca tatgggtgtt
     3481 tatctcagag atgatagaat agtttctagg ataaaaagta gcagctacga tgttatcctt
     3541 cgtagaggat tcctgcttca gctagttttt gtttgatgtt tttcagcata tgcttttctg
     3601 ggtagcggat cgtatgcaga tggatgccgc ctgttaattc ggataacaaa gtagcattac
     3661 ttttttgata acgtctcata aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc
     3721 ctgctaactc accgtaaagg ggatgttcca caattacatc aatgatttca ccaccatttt
     3781 caacgattag tctcagttct tcttctgttt gatcttttcc atgctggaca gcgattttac
     3841 tgatgtgccc agtattggtt tcttctgttt cgaggatata acctcttgct gttgccacga
     3901 tacttttacc tgcagctcgt agtaaagcaa tatcgcctac aatgatctga cggctgactc
     3961 caaaagtttt cgcaaacttg ctagcactca ctggtttttc tgtatttgcc aaggtttgca
     4021 tgatttgttt tcgccgtgtc tctccttcga tcacacttat tcctccttca ttttgttttg
     4081 gtattcgcgc gagtgtttca tcaaatcaac atacgtatct gttattgttt ccttatatcc
     4141 cttatttttc agcagactaa caactttatc caatacttgc tgctctcttt ttgcatcata
     4201 aactgcttgg ttttcttctt ttttcacttg tcctatttta gttaccgtat ccattctttt
     4261 ttctaataaa gcaatcaact ctcgatcgat catatcgatc ttatgacgta atgtttcaag
     4321 agactcttct gttagttctt cctctactgt cttgatgata cgagcaggat ttcctgcaat
     4381 cactacattg tcgggaaaag atttggtcac aaccgaacct gcgccgacta ctacgttgtc
     4441 ccctagagtg acacccggcg tgacgataac tcctccgcct aaccaaacat tattaccgat
     4501 tttaatgggt tttgcgtact ccagaccgct gttacgtttc actggatgta gtggatgcgt
     4561 cgccgtgtaa agctggacat tcggagcgaa catgcagtta tcaccgattt caatcgtgct
     4621 gacatctaaa aatgtacaat tgaaatttgc ataaaaattc tccccaacaa atatattata
     4681 tccgtaatca aaattgataa ctggctccat atagattttt ttccctgtac ggccaaaagt
     4741 ctctttcaac aattgggatc gcagttccgc tgattctgct tggttgatga tttggctttg
     4801 acttcttgca aacatacggt cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc
     4861 tgcaatcatt ttttcttttt cggttttgaa ttcagtcata atctgctcct aattcttgta
     4921 tttttttgaa aacaaccgct ccgtcttctt gaataaacgt ataaggctcc gctaaataaa
     4981 caggcgtttg attgattact aagattttgg ctgacggctg gcgaaactgt accagatcac
     5041 aaaacggatg aacttgaaaa ctcgttccaa caatgacgat cagctctgct tggctgacgg
     5101 cataagccgc ctgctccatg actccatctt gaaacccttc accatataac acgatgtctg
     5161 ggcgtatttg tcctccgcat gcttgatgtt tgtcgctgtg aagatattct ttccagtcta
     5221 ccgtttgttg acattttcga caatgacatt gatacaaact tccatgaaaa ttgacgagtt
     5281 gcgggctttt agcctttgcg tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt
     5341 tttctttcgc tagatgtgcc atttccaaat gaatgatatt gggttttgct tcaggatgat
     5401 aaatcttttt gacaaattga tagaatttat ccggttcatt caccattgct tgatgactca
     5461 gcaagtattc cggctgctcc atcccgtggt agattcctga caaagatcga tagtctggca
     5521 ctccagaagg tgtggatacc cctgcaccgg tcaaaaatgt aatttgctga taagctaata
     5581 tctcttttgc tgcttcgtct actgttaatt gtttcatgtt ttgtcacctc actttccatt
     5641 atagcccata ctcgaacaaa tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg
     5701 ttatgggcat ttgaacaatt tatcacaatg ttcactgaca aaagaatgaa aacgtgttac
     5761 aatactgctg tataaaagtg aggtgaatcg atatgttacc attctttatt tattggggcg
     5821 ttattctggc ttgtatcgcc tggttagcgt taagcgttta tttctctgtt ttttatcttg
     5881 tacgcaaaga aaatggaaat ctttgggctt ttgctttatt caacgtgatt gccgcaattg
     5941 ttttagccat tacattagcc gtatatcgaa catggggctg gggaatcacc caatactcaa
     6001 gtttgattta tctaatatta gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag
     6061 gacgtgagcc aaagaaagcg gcagcttaaa tgatcgagat ggaactggac acaggaaatt
     6121 ttctgttcta gttctttttt tattgtatta catacatttt tgtgtataat tatactatcg
     6181 tttgcaatgg atagatgatt cacattttga ttgttttttc ggtaaaagta aagatttcta
     6241 tatttatcag tattttttca aaaaacagca gttatagatt gacaaagcct ctcgagtatc
     6301 gtaaactttt tttgtataaa taactgaata cgagatgcct caatcaatga ggtagaggtc
     6361 gcgagaatca ttaaccctgt ggagtgaagc aaacatggag aagcaggaat agggaggatc
     6421 gccgaaatgt gcactttttt gcttggagtg tatgttggga cgcaagttaa gagcttgcgg
     6481 actgtcttag cagtgatgct aagttgcgct atgttttgtg agaggtttat caccttatgg
     6541 aattttagcg gcctttttat atctataaat cggtcgcttt ttattttttc agataaagct
     6601 ttaccgcatt tcatctagac cgctcgtgta tcatataatc agaaagggag aaaaaaatga
     6661 aaaaaaatat ggtttcagtt gccgtactat ttgcttttgt cttctcacta ttcagtgtgg
     6721 gatctctggc aaatgcagat gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg
     6781 ctgggtatgc acccttcaac tggtctcaac aaacagatga gaataatgcc ttgccaattc
     6841 aaggccaaaa tagttacgcc ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg
     6901 gtttagggaa aaaattagtc atcgtccaaa cgaaatggga tggtctggct cctgcccttc
     6961 aatcaggcaa gatcgacgcg atcatcgccg gaatgagccc tactgctgaa cgacgaaaag
     7021 aaattgattt taccgatcct tattacgaat cacaattagt agtagtagtt caaaaagata
     7081 gcaaatatgc aaaagcaaaa agtttagcag atctttctgg agcaaagatt actgctcagt
     7141 taaatacatt ccactatact gtgatcgatc agataccaga tgtgcagaaa caacaagcaa
     7201 tggataattt ctcagcaatg agaacagctt tagcttctgg aatgatcgat ggttatgtca
     7261 gcgaacgtcc ggaaggtgta acggcaacta gtgtgaacaa agacttggaa atgcttgaat
     7321 tcagcggtgc agatggcttc cagacaaacc cggaagatgt tcagatttct gttggtatgc
     7381 gtaaaggcga cccggatatc caaaaagtaa accagattct ttctggcatc tctaacgatg
     7441 aacgaaccaa aattatggac caagcagtca aagatcagcc tgctgcaact gatagtgaag
     7501 aaaaacctgg catcatttct gatttcacta cgatttggaa acaatacggc aatatgttct
     7561 tacgaggtgc tggacttacc ttgttcatcg cattgatcgg gacagttgtc ggtactacac
     7621 ttggattact gattggtgtc ttccgttcgg tacctgaatc aggaaatgca ctgactcgct
     7681 tcttccaaaa aatcggaaat gcacttcttt ctatctatgt cgaagtattc cgcggaacac
     7741 cgatgatggt acaagcaatg gtcatcttct acggtctggc actcgctttt ggcatttcat
     7801 tgaaccgaac gattgccgct ttgtttattg tctctctcaa tactggagct tacatgacag
     7861 agatcgttcg tggggggatt tttgcagtag atgaagggca gttcgaagct gcgcaagcca
     7921 ttggtatgac ccatggccaa acgatgagaa aagttgttat tccacaagtt ttacggaata
     7981 tcttgccagc aacaggaaat gagtttgtta tcaatatcaa agatacagca gttttaagtg
     8041 taatcggcgt agctgatttg ttcttccaag gaaactctgc ttccggtgcg aatttccaat
     8101 tcttccaaac atttacgatc gtcgggatca tctacttagt catgaccttt acgatcacca
     8161 gaatcctgcg ggcggtagaa aagaaaatcg atggtccttc agcttatacc aagcttgaag
     8221 aaatcgacaa ttcaaatttg caggaataag gaggaagaat catgaatgaa aaaattattg
     8281 aaatcaaaca tctaagaaaa agcttcggtg aaaatgaagt attgaaagat atcagcgtaa
     8341 cagtcaataa aggtgaagtt gtgacgatca tcggttcttc cggttctggt aaatctactt
     8401 tcttgcgctg tatcaattta ctggaaaaac caacaggcgg cgagatcatt tataatggag
     8461 aaaatgtatt agctcctaaa tataatttac ctaaatatcg aacgaatcta ggtatggttt
     8521 tccaatcctt caacctgttc aacaatatga atgtcttgga aaactgtatg tctggtcaaa
     8581 caactgtcct gaaacgagat aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa
     8641 aagttggtat ggatcgttat atcgaagcca aaccagcaca attatctggt ggacagaaac
     8701 agcgtgtcgc aattgctcgg gcattatcaa tgaatccaga tgtattgcta ttcgacgaac
     8761 ctacttcggc gttagaccct gaaatggttg gcgaagtgct taatacgatc aaagacttag
     8821 cacatactgg cttgacaatg cttatcgtga cgcatgagat ggaatttgca aaagatgtat
     8881 ctgaccgtgt catttttatg gataaaggtg tgattgcaga agaaggtaca ccagaagata
     8941 tctttgtcca tccgaaagaa gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt
     9001 aggatcaata gctgattggg acaaagagct gcggtgttta ccgcagttct tttttatgaa
     9061 ttactcataa aaggtaaatt tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac
     9121 tcttttaata tggaagtaat taaacttttt tagttaggat gatttaaaaa tgaatgaaga
     9181 gcaagaactg cagcgtggac tgaaaaatag acatgtccaa ctgatttcta ttggcggagc
     9241 gattggaacg gggttgtttt taggttccgg aaagtctatc cagctagctg gcccttcgat
     9301 attgctcgcc tatcttatta ctggatgcat ctgttttttc attatgcgtg cgttaggcga
     9361 acttctgtta tcaaacacca ataaccattc ttttctggat tttgttgcag aatatcttgg
     9421 caaaaaagcg gctttcatca ctggctggac gtattggttc tgctgggtat cgattgcaat
     9481 ggccgattta acagccatcg gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg
     9541 gcttcctgaa ctgattgcat taggtcttct tctttgcttg aatatggtgg ctgtctcatt
     9601 gtttggtgag ttagagtttt ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat
     9661 cctcgtagga gcttatatga tcctcaccca ctatacgaca gatattggca cagccagtat
     9721 cacgaatcta tggtctcatg gcggattctt tccaactggt gcaaaaggat ttatcttagc
     9781 tttccaaatg gtaacctttg cttttgctgg gatagaatta gttggattag tcgctggaga
     9841 aacagaaaat cctgaaaaag tgttgccgga agctatcaat aatattccta tcaggatcat
     9901 cttattttac ttaggatctc tttttgtcat tatggcaatc tatccatgga acagcttgaa
     9961 tccagataac agtccatttg tagaagtatt ttcggaaatt gggatcacga ttgctgcttc
    10021 cttgatcaat ttagtcgtat taagtgcagc agcttctgcc tgcaacagtg caatatatag
    10081 tacaggacgt atgcttcgtt ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa
    10141 gttaacaaca catcatgtgc cagggaatgc tttgacattt tctactatcg tcattttcat
    10201 ttctgttatc ttgaattacg tgatgcctag tgaagtattc acactagtaa gcagtattgc
    10261 cactacttgt ttcttgttta tctggagtat gcttgtctac acgcacatga aatatcgtaa
    10321 aagtatcctc ggcaaaaaag ctcattcatt taaaatgccg ttgtacccat ttagtaatta
    10381 tctggtattt ctttacatgg cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag
    10441 aatcgcttta ttactaacac ctgtgtggtt cttgcttcta ttactaatct atcatatgaa
    10501 atatgaacgt aagaagtaag aaagtagttg tgacagaagt ggtcagcttc gagaaataag
    10561 aaggaattta cgaaaattgc ttcttaaatt tttgtgaaat ttcggcttat ttcttggagt
    10621 taaaagcttc tgtcccagcc tcttttctat ttccccggaa acaaccgctg ataataggca
    10681 tctgcttttt tataggaaaa aatccaaata ccagatacaa aaaaacgtcc gaataacccg
    10741 atcaacggta caaaaaagac accggcaatc aagcataagt taatcatcat ggagataaga
    10801 cttcgacggt cctttgacaa aactctcacg acatgggaat ctggtttatt tgctttcatg
    10861 atttgcctca tcaagatacc aaaagaaaaa ctccacatcg tgtatactaa gacataagcc
    10921 atctcaggcc aaaaagcttc agggttacgc ccgacccaac tagtaacaga aggacataaa
    10981 gtcagccaaa agagcaaaaa gatattcgac cagaccactc ggtaattaat tcgttctgcc
    11041 atctgaaata tacgatgatg gttcacccag atcaccgcaa caaatataaa gctgatggta
    11101 taagctaata atgtatttcg gatgacccat aaagaatgaa agctagatgt attagggatc
    11161 ttgatctcta atacaagaac tctcaaaaca atggctacca ctccatcagt aaatgcttct
    11221 aatctcgact ttgtcacgat gattcctcct cagatgattc agcgctttta ttttatcact
    11281 tacgttatta aataaccgtc gttttatata ggaaattttc tgaaaattgt tgcatatttt
    11341 tcgaaatttt gttactttta aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc
    11401 atttaaagac tatcttttta taggctccat gcttttcggt ctattttttg gtgctggaaa
    11461 tcttatcttt cccgttcatt tgggtcaaga agcaggcgct gctacctttt gggcgaatct
    11521 aggtttcttg gttaccggta tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc
    11581 gaaaagctca ggtgtatatg aattagcaaa acggataaat caaacatatg cgatggtttt
    11641 taccatcttg ctttacttag ttatcggacc gttttttgct ttacctcgcc ttgcaaccac
    11701 atctttcgag attggtttag caccatttat cgatcctagc agacagacgc tatatctagc
    11761 aattttcagt attgtatttt ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat
    11821 attagattat gttggaaagt tcctgaatcc tgcatttttg gttttattag gtgctttgct
    11881 tctccttgct tttttgaatc cactaggttc tgtaaatcaa gcgcctattc agccaaatta
    11941 tcaagagcat gcttttttca cagggtttac tcaaggatat aacacattag atgcgcttgc
    12001 tgctttagca tttgggatca tcattgtcac aacaatccaa aacatgggtg taacaaaacc
    12061 tgctgaaatc gctaaagaca cgatcaaatc tggtgcgatc agcattatat tgatgggaat
    12121 catttatact ttgctcgctt atttaggtgc tatgagctta ggtagctttg ctctaagtga
    12181 aaatggcggg atcacgctgg ctcagattgc tgatcattat ttaggtactt atggcagtat
    12241 cttattagca tttatcgtta tattagcttg cttgaagacc ggtattggtt tgattacagc
    12301 ttttgcagaa acattcacag atttgtttcc gcaaagaaat tacgcattct ttgttgcact
    12361 tgctagtatc cttccttgtt tagcggcaaa cgtaggcttg acgaatatca ttcaattttc
    12421 gttgcctgtt ttgatgttca tctatccatt agcaatgaca ctgattttac tagtgttagt
    12481 tggtcctttg tttaagcagc gtacggctgt ttatcgaatg actacttatt ttacattgat
    12541 tgcttcgatt tttgatgggt tgaacgcttg cccagaaact atcaaacaaa cacctattgt
    12601 tcaaaatctt ttatatgttg cggaaagcta cttgccgttt ttcaaattgg ggatggggtg
    12661 gatcgtacca gcagttatcg gttttgttat cggactgatt tggagtttcg ctaagaaaga
    12721 agaagtagca gattgataaa agaggttgtg aaagagacgt tcagccttat tgccgatagg
    12781 ctgtctcttg aacactgttt attgagaggt cgtgaaaaag ccgctcagtt tcgagtatta
    12841 agaaatattt tttgaaaatg gcttctcaca tttttgcata aatttgactt aataccgaga
    12901 aactggcttt tgaacaccgt ttattgagcg tctataaact gacctgagta ttagagaaac
    12961 aaaacggcaa agtagctcca tatactttgc cgttttgttt tgaactaata ccgaagagaa
    13021 gctgatcaaa caacatttct tttataatat agattttaga catatatatg atataaaaat
    13081 agttttcgca aaaaaa
//
LOCUS       DASJON010000068        13057 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJON010000068 DASJON010000000
VERSION     DASJON010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.068"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(245..859)
                     /locus_tag="U3989_001437"
     CDS             complement(245..859)
                     /locus_tag="U3989_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1714892.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1465..1842)
                     /locus_tag="U3989_001438"
     CDS             complement(1465..1842)
                     /locus_tag="U3989_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714893.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2142..3023)
                     /gene="galU"
                     /locus_tag="U3989_001439"
     CDS             complement(2142..3023)
                     /gene="galU"
                     /locus_tag="U3989_001439"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1714894.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3066..4085)
                     /locus_tag="U3989_001440"
     CDS             complement(3066..4085)
                     /locus_tag="U3989_001440"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1714895.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4718..5722)
                     /locus_tag="U3989_001441"
     CDS             complement(4718..5722)
                     /locus_tag="U3989_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1714896.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5735..6784)
                     /locus_tag="U3989_001442"
     CDS             complement(5735..6784)
                     /locus_tag="U3989_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1714897.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6801..7622)
                     /locus_tag="U3989_001443"
     CDS             complement(6801..7622)
                     /locus_tag="U3989_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1714898.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7609..8388)
                     /locus_tag="U3989_001444"
     CDS             complement(7609..8388)
                     /locus_tag="U3989_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1714899.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8405..8875)
                     /locus_tag="U3989_001445"
     CDS             complement(8405..8875)
                     /locus_tag="U3989_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1714900.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8887..9306)
                     /locus_tag="U3989_001446"
     CDS             complement(8887..9306)
                     /locus_tag="U3989_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN1714901.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9387..12164)
                     /locus_tag="U3989_001447"
     CDS             complement(9387..12164)
                     /locus_tag="U3989_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1714902.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12355..12579
                     /locus_tag="U3989_001448"
     CDS             12355..12579
                     /locus_tag="U3989_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714903.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3831 a         2732 c         2121 g         4373 t
ORIGIN      
        1 tactttttat ttttctgaca ctacatattc ttataactca tttctcataa ctgtgaaaca
       61 acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg atccatctta
      121 aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg ttttcttttt
      181 ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt caacatgtgg
      241 ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac aaaacttgct
      301 gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct agatttggca
      361 aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa
      421 ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca aatccagtaa
      481 caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg attgccgggt
      541 ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa agctgatcaa
      601 taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga ttttcaatca
      661 atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca ccacttagtg
      721 tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg atccaagtgc
      781 cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg tgataatagt
      841 caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg aatagaaaca
      901 gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca catgtggtct
      961 ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt gacatagcaa
     1021 ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt caccctagat
     1081 taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat cggtcagggt
     1141 gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca gggagccttt
     1201 ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca agtgcaagca
     1261 agaaaataca aaaaaacatt ggtatgattt atatatatat aaataacata aaaaaaggaa
     1321 tggcttatcc cgatacaaca agcattcctt taaaaaagat tcttttatat aagtgaaaaa
     1381 agcttttttc caccaattaa ataaatctct catctcaata gtttatccat tattttatct
     1441 ttttttgcgt atacctacta aatattaccg tcttcttctc gttcttctac tttgttcgag
     1501 agaatctgta ctagaaaaag tagttgtatg tctacctttt ctgcctatcc gagactttct
     1561 tttcatatat gggatataaa tgattagata cagtacagca aaaaataaag caaatacgca
     1621 agcttcccaa ctacctaacg ccaattcaac catcaaggta gcaaccccaa cggatgctgc
     1681 ataccagata aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca tgataaatag
     1741 actaataagc aatagtggca acacaaatat ccaattgctt ccttccatca gccacttgag
     1801 tatatcaagc agcgactgtg caacagcgac taattttccc atagtgaaca cacacctttt
     1861 tttataatta aaaaattcgc ctttttattc tactatgaat cttctggctt ggctttcatt
     1921 tctcttatgt aatcattttc ttttatacaa cttaaaaaaa aattaaacca ttagcataat
     1981 attttctgaa tctattcacg aaagtctttt tctatctttc gttttccacg actcaactga
     2041 tctagagtca aaaaactaaa acgacagttg cgcaaatgac gcacaagctg ccgctttaat
     2101 aacaaatagc ctttcattct cagcaggcca cttatttcca gttaactttc tgttcttagc
     2161 tttttactca actggataat atatgcactt aatgaagtcc caatttccga atgttgaagt
     2221 ccatattcaa tatttgtctt catatatcct aacttatcgc ctacgtcgta tctttttcct
     2281 ttaaactctc tcgcatagac cgcctctgtc tggttaagag tctgaagtgc atccgttagc
     2341 tgtatctcgt ttcctgctcc tggttcttga gtttctaata tctcaaatat tttaggagtc
     2401 agtagataac gtccgataat ggctaaatta cttggtgctt ttcccatttc aggtttttct
     2461 acaaaccctt ttactctgca acgacccttt tcgctatctg ttttaggatc aataattccg
     2521 tatttagatg tttcattttc tggtacacgc atgacggcaa cagcggaagc ttgtgtttct
     2581 tcataatcca tcatcaattg ttgagtcaat gggatatcat ctttcatcaa atcatccccc
     2641 agcatgacga caaacggttc atttcctata aatgcttttg cttgaagcac ggcatccccc
     2701 agtcctcttg gatgagattg acggatgaag tgaagattga cttgagtcgt ttcttcaact
     2761 aaagcgagta attctgtttt ttccttttcc cgcaaattgt tttccaattc gatatttgca
     2821 tcaaagtgat cttcaatggg tcttttttct tttcccgtta cgatcaagat gtcttcgatt
     2881 cccgaatcca atgcttcttc cacaataaac tggatcgttg gtttatccac gattggaagc
     2941 atttcttttg ccatggcttt tgttgccggc aaaaaacgtg ttcctagccc agcggcagga
     3001 atgactgctt ttttgacctt cacttgctgt tcctcctaaa cgacgattaa ctattgatca
     3061 tctatttaac gaagagaaaa ttcattctcc atttttccgt cacgtaacat gatttctttt
     3121 gctgcttttt tgatatcttc tccattatac aaaacattat agattgtttg agtaattggc
     3181 atctcaacac ctagctgttg agctagctct actgctgctt ttgtcgttgc cacaccctca
     3241 acgaccattc ccatgttttc tagaacttct tccagcttat gtccctgtcc taacaagtta
     3301 ccggcacgcc aattgcgaga atgaacactt gtacaagtaa cgatcaagtc ccccacacca
     3361 ctcaaaccga taaatgtcag aggattggca cccatcgcga caccaagacg gctgatttct
     3421 gccaatcctc gagtcatgat tgctgctttt gcatcatctc cgaatcctaa tccatggata
     3481 gcgccggcac caattgcaat gatatttttc agtgctgcac cagtttctac gccaatcaca
     3541 tcattatttg tataaatcct gaaataatca ttcatgaaca gtttttgtac ataggctgct
     3601 gctttttgat caatacttgc agctgtaatt gttgtgatat catgtactgc cacttcctca
     3661 gcatggctag gaccagataa tacaacaatt gcctgtcttt tttcttctgg aatttcttca
     3721 gccaaaactt ctgagattcg tttatgcgta tcttgttcta gtcctttact tgcatgtata
     3781 atgattggct tagtattcat tttaccaata agttcctgtg cgactgaacg aatcgctttt
     3841 gttggaacaa caaataaaat agcatctgcg ttatccactg cttctgccaa ttctttatat
     3901 ccgataatcg attctgggat cgtcaaatca ggtaaaaaat gctgatttgt atgatactta
     3961 ttgatttcat caatctgttc aggtacattt ccccaaatac ggacatcatg cccgttttct
     4021 gccaatgttt gtgctaaagc agtgccccaa gatccaggac caagaactgc gattttttgt
     4081 ttcatggata ttcctccaaa taaataattg ataatttcca ttatagtata catttcttaa
     4141 agttgggact aatttccatt gtttttaagt gtttgtttac ttcttttatc gtttttactt
     4201 gaaataatca atgatatgtt acaatagtct acattttatt tttacactcg ttatgctttg
     4261 cttgttccta agaggaagca gttttgacaa tgttactacc agcattacta ttaggaggtt
     4321 aggaaacttc tcctagtgag tattgattta aaatacataa gagacagatt gaacatttct
     4381 aaatctgtca ggtcctgcaa cggacagatt gtctctgttg acggttgaca taggaacttt
     4441 tgttttagtc aacattctgg atacaaaacg ctatgtaaac tggcgcaact cgttgtactt
     4501 ggacttttac tacggatcca tgcaacaact aaatagtact attgcataac aaaaaatggt
     4561 tggcacccac atgtcccatt aaacagatta taagttgaac tgtagataat gcaaaaaatg
     4621 tgttttataa cgtaattgat gccaatattt taaaaaaata aagggagcag ttatcgctgc
     4681 tctctttatt ttttatattc tttttatcca gtctatccta ataatcaaac tgacgatagt
     4741 agcggcgtat ctctaatgga tggcagttca actgctccac atgagcatcg atgcgttgtg
     4801 tcactacatg catcaataag aaagacaaaa cacgtccgcg atatttttca ctgatacctt
     4861 ctactggata gtcttttgtg tctatcaatg tataattgct gcaaattttt ggcaaaagtt
     4921 tagccacacg ttcagataac actctctgtt catcttcgcc taaaaataat gtgactgggg
     4981 ttgtttcttc tacgatctct aacgtcccgt gaagaaactc ggcagcatgg atagatttcg
     5041 atggaagcca gctttgttct tcccagtaac acatggcata tgaataaaca gctccccatt
     5101 gattccctgc tccaataaaa taatgcatac tatcacgtcg atgtttttct gcaaatgata
     5161 agccaaatgc atctgctttt ttctctgctt ccactaaccc tgttggtaaa taacgttcga
     5221 gctgttcata gtattctgaa taagcttcga attctccatt caaatacatc aatcgatctg
     5281 ctgccataaa aaatttgatt tgttctgttc caggagctgg ataagtgacc acaaaatcgc
     5341 atagctgaga caacttactg ttcgctttat cgataaatcc aatcagtgtc gctcctactt
     5401 tcttgatttg ttctactgct ttcactactt cttgagttgt accggtaaca gaggacaaaa
     5461 taacgataga atcttttgtt aatcttttgt ttccagtagt gtaatattct gcagcatgct
     5521 gtacaaagac aggcaagttg cttttcccat tcatataagt gacagcttgc atcgaggaag
     5581 catacgttcc accgattcct agataataga cagcgtcaaa tttttccgca taaagtttat
     5641 cgatgatttc ttctacttgc ggacgtaaag ctagtgcgcc ttttattgct ttgatttgtt
     5701 cttcttcatt gaatttcagc atgatttcca cctcttataa tttacttttc aagactgtat
     5761 caaagtcttc aaaaatcttt ttgttcaagt caataccttt agctactgcg gttttataag
     5821 cgaatagttg aaacggaacg acaagcgcta atacagacag atattcatca cattctattg
     5881 ctaatccaag tgttttctcg tttcttgctt tttttgtggt aatggtgaga gtctctccaa
     5941 cgtaccgact catatatctt tgcaactttt gcgaacgttc cttatttaca ctttcccctt
     6001 caataaagaa tagcaaatgc gaagcatctg cctccaggta tgggccgtgc atataagctt
     6061 ctaactcaaa tccttgtgac ggaacgcgga ttgtttcagt caatttcgtc tctgcttctt
     6121 tagctgttcc ccagtttgga ccatatccaa tggtgacgaa acgctgtgct aaacgaaata
     6181 aactttgata tccatcaaaa aattcttccg ttttttgaat aatcgctggt aaatggttga
     6241 ttattttgcg caactgttgc atgtagtctt gttctatttt tttgctgatt ttatttttac
     6301 ttattccaat tcctatccct aagagtagta actgtaaaac agtcgcagaa tagccttttg
     6361 taacgaagcc tactgattct accccggcac ctaaatcaat aatttgtgca gcttctaaag
     6421 caagtggact ttgcacgtca tttgtgatag caacaacagg taacccttgt ttttgtatca
     6481 tttttactgc ttcaacagta gaagcacttt tcccgctttg tgacacagca atcacagtat
     6541 cgattgaagg agaaagtttt ccataattga aaaaataaaa aggttcttga atatcgacag
     6601 taagatcacc ataagattcc aatgctggct tcgctgctaa acaagcgtta taagatgaac
     6661 ccgttgctaa gatcagtaaa ttcactgttt ttttcatatg ttctcttgtc gaaaaatcat
     6721 tctttttcaa gatcgtactt agtacttcct tttcttcata gatataatct tgcatcgtaa
     6781 acattttctc tgctcctttt ttaaaaaatc cctatccacg aaccaaaaat tccaaagaaa
     6841 gctagtccga tcaagatcgt taatggtttc acttctttct tcaatagata aaacactatg
     6901 ccaaatgctc caagacataa gatatttgga acgatatcat caaaaatact ttgaacagtc
     6961 acagcatttt ctcctgaacc gatttttaaa ggaatattga tggtgatcat cgtggcagtc
     7021 attgctccaa cgaccatcag tccaataata gacgcgccta atgtcaaact ttccatcata
     7081 ccgtttgcct ggatcttttt caaaaaaccc gttcctaagc gatatcccca acctgttgct
     7141 agatagcgaa acagaatgtg cggaatattg aaaatcaaaa tgaacaagat agggcctaaa
     7201 atatttcctt gcatggctaa agaggtaccc acacctgtgg caatcaatcg caaagttccc
     7261 caaaagaaag aatctcctaa ccctgcaaga ggtcccatca aagatgtttt gatactatcg
     7321 atcgcagtca catcaaattc aggatcatcc ttattttctt cctccattgc agccgtgatt
     7381 cctaaaatca gtgtaacgat atgtggggtc gtgttaaaaa actctagatg ccgtttcaaa
     7441 gctgctgcca tttcttcttt cgactgatat aactttttca aaatcggaat cattgcataa
     7501 acataagcca tattcatctg acgttcataa ttccaagaaa actccatttg aaaagaacgc
     7561 caaaatacgt gatttagctc ttttttcgtt attttgctgt ttttgatctc agaattcttc
     7621 atcttcttct acctcccctg cagttgtttg aagtggtagt tgacggacat tcataatatt
     7681 gactaccaca actgctaaaa tcgcaccaaa aatagctact ccggtcacag gaatctctaa
     7741 gtaagaagct atagcaaaac ctaagaaaaa atatggtgca acttgtttgt tgatcagcaa
     7801 acgagcaagc atcgcaaaac caagagctgg aatcaaccct gtagctactg acaacccctt
     7861 ttgaacgaag tcaggaatca tatttaatag atttccgatc gtattgcttc ccaccatata
     7921 agaaaccatt accacaacac caagcatcaa ggaaagaccg ataccggcaa gcaagtgcat
     7981 atgctcgact cctttataat ttccttcttc tgcataattg tctgctttat ggttcataat
     8041 tggaatcaaa agccctaagt aaatattttt caatatcaat gtcaacgtag caatcggcaa
     8101 acctagtaaa agcgcggttt ctgtaccggc tccagctgta atagcaaaag ccgtccctaa
     8161 aatcccaccg gtcacaacat caggtggaat cgaagcacct actgagaacg atccgataaa
     8221 tgctaactct aaagttgcgc ccataatgat cccagttttt acatcaccta acacgattcc
     8281 tgtaaacagc cccgtcacaa tcggacgaga aagcaacgat gtccctagtg catattctga
     8341 ttgtgcgaca aatgcaacaa ttcctaataa gactgcttgt acaatcattg aggttcttcc
     8401 tttcttacaa aacttctctt acatccactt ttttatctgc tggtacttga cggatttcta
     8461 cttctatttg tttgtcaatt aatcgtttca agcgttcctc ctcttctggt aaaatattta
     8521 ccgcttttcc gatgctgcga gtaccaggtt ttgccttgat ccctcctaag ttgatggttt
     8581 tgatttctgg atacgcttct gccagcttat atgcatcctc gatcgattcc actacgatga
     8641 ataacttata tttatctgtc accccgcttt tcaacgcggc aatcgagtca tcaattgatt
     8701 tgatcactag tttgactcct tgtggcttgg ctaatttgat cgttgtcttt cgtatctcat
     8761 tcgtaggtac atcatcatta gcaatcaaaa tacagtctgc gcctattgtt tgtgtccatg
     8821 aaaaagctac ttgtccgtgt aataaccgat ggtcgactct tgtcaataca atcatttttc
     8881 ttcctcctaa aattcgtctt cttctatttt ctgattgagc cgctcgttac aaaactggat
     8941 catttttttt gaatcagtta gcacctgctg gatcaaaaca gttgtctcta cttcagcgtc
     9001 caaccgagat actagctcaa tcaatagtgg caaattcaat ccggcaacga gatagacatg
     9061 atcccgattg atatattgta agaattcgtt gttgacactt ccgccaaaaa gatccgtcag
     9121 aacaatgatt tctttatctt tgtacctcat aaaaagctgc cttactgttt ccgctaaatc
     9181 aaaattctca tcagtatagc aatcgaatac agtaatagaa tgatttttcc catagatcag
     9241 ctctaatgaa tctaatatcc ctgaagcaaa ttttccatgg gaagctaaga taatttctct
     9301 atccactttt tcatctcctc ttttcactta cacatactaa aaaagcaagt tgcatgccaa
     9361 aattagtggc aacaacttgc ttatgttcaa aattcttctt ctatcgagag aaactctgta
     9421 tcttcactca aaatatcata gatataggca atctcagaat tggggatcat gacactataa
     9481 tgctcttcaa tgacactaaa cgcagattta atagcagata acccttcttt ttgacactgc
     9541 ttccatttct ctaatccttg ataggtttcg attggcattt gtcggatcaa tcgttcaatg
     9601 agacagctca catggacata caacgctaat tttttgggat tgctgatgct tcgattcaac
     9661 tgcacctcta attctctcat aaaatgatcg atttctccca tcaccttctc tgtatctaga
     9721 atagtcacgg aatccagtac tttttccaat gaaaaattac gaatgatgcg ctgattgaat
     9781 atttccttct ctgaagcatt catagtagtg tccaaccatt cagctagtac agacatcttt
     9841 tcccccgaga tgaggtcttc taatgaaaga tagtcgatat tggtgatatc aggatctgtg
     9901 gtgccaatca ttcctacgac ttcgtatatg gaaaataatg ggtcactgtt tttcttatct
     9961 tttaattgct gatattcata tgggatgatt ttcaactgac aagccgtagg taagcttttt
    10021 tctagcagtt cacttaagtg gaccgccgta ccgattccag tgaagcaagt agtaatgagt
    10081 gctttgggtt tgttttcctc tggatagagc agtcgccatt ctggctggat ctgtcgaaca
    10141 gtctcttctg ctatctcttc taatgataca tttttttgca ggcattcccc tactgtgatt
    10201 gctaaaggtg tcgtcacatt attcaaaatc aaaagcggta cggataactg ttttttaaaa
    10261 aactgatgga tttctttcag agatcccatg tctactaata tgatcaatcc atttgaaaca
    10321 tcgctgtttt cgcaataatc aaggatttct gaagcaatct gctgtggtgt gacatctagt
    10381 ggcatatcaa atgattcaaa tagatcctct tgtaataaac gatttactac atttgccata
    10441 ctgctagcag ttgcataacc gtgagcagca atcatcgctt ttggcagtcg tttttctttt
    10501 gaccatttcg ctttttttag atagatcgtc agtaaaatcc gatccatact tgtgtcttca
    10561 atatctaatt tgatcttact taattccaga attcgttgga catagtgata actagatgga
    10621 taagactgag aaacttgttg ttctaattct ttgattagtt ggataatatc atgttcttcc
    10681 ggtaaccatt tgatagtgct tcgctgatag agatagtaac tcaaggcata gacactgttt
    10741 ccatcgaatt tgatttgata ggcagtttcc atttgtcgaa aggtctctcg gatagattgt
    10801 gtccaataaa ccagcagctc gtgtttttct tgtctactgg tctcaaacag caaaaaatca
    10861 aataattgaa tcacttcttg ctttaatttt ccttcgcaag aatatagcag acagttattt
    10921 ttcttgaaat caattaggat ttgctcaaaa cttttaacga ttcgctgctg ttcgggctgt
    10981 tgcttctcca ttaattgctc taaggtctgg tcttgcgtaa tatagattcc ttgctgtgct
    11041 gtatttttag taataggctg cgcaagtagt tctttttgca aatgatggat cgtgacatgg
    11101 atttcaggct gttcacgctg ttcagcatat gcttttgctg ctgaaacttt gacgatattt
    11161 ttcagctctc ctatgtttcc ggataatttc tgacttgtta aaagtgacaa agctggtcca
    11221 ctgactgaca aagggctccc catttttctt tgttcttcga gaaaaaaaga gtaaatcagt
    11281 tctagccgtt cattacgact acgctgatta agtgcaggta gttcgattcg gataggtatt
    11341 cttcgaatga aagttgttaa gaaatgacta gtaatctctt cagtcgtagc aaaaaagaga
    11401 cgtgttttga tccggcgtga caaattaggt tctcccatac gataaatcac accttgatca
    11461 agataggtga acagtttttc ctgaccttct ggtgttaagc gatgtacctc gtctaaaaac
    11521 aagatcccac catctgctgc ttcaaaagcc ccttttttat tttcttctgc accagtgtac
    11581 gctcccttaa catgtccaaa caaattgcta gtcaaaagtt caggattatc tgcatattgt
    11641 gcacaattaa aagtgatgaa tggtgcagaa tcttctagca gatcatgaag cagacaatac
    11701 tgatagacca aattaactaa ataactttta cctgtgccac tttcgcctgt aatcaaaagt
    11761 ggtaatccgc catctggata gtaaagagct gttttgattt gttcgatact ttctttcaaa
    11821 ctggcgtcgt atccgatcat aaaagagaat aagtcttttg gttgcgcgaa aaaaggctgt
    11881 tcctctttga gatcatcgat agaaaaataa aaattcgtgc gtaacttaaa aaattgtttt
    11941 tcaaaagctt ctttgtggaa ataaatcact ggtcgagaat tgattttgac tagtagttgc
    12001 tcatcattta actggttcaa ataatgactg actgtgtttc tttttacccc aaaatgatca
    12061 gctaaccttt gtgctgtaaa gacctcgttc atatgttcta aatcgaaaaa ggctgtctgg
    12121 ttctctaagt aacataaaat ttccttcttc aacaacttat tcacccctcc tccatcatag
    12181 aaaacggatt cattttgtca ataaaatctt taataaagaa ttgaaaaata tatcttatta
    12241 tagacagcag cctacttatc ccttataatt tttattctaa aaaacgtaca gttccttcca
    12301 gtttttatac tggaatttca acatatgaaa gaataaggag gctatgacta ttcaatgaaa
    12361 tcggataaat ataaaaacag gaaacatact tcctggaaaa cttttcgaga aatcatcgga
    12421 tcttatctcg ctgtagttgt atttttgcct ttgctttata tggctatgac agaacaaact
    12481 cgttttattg gcgtagatac gcaatatata gccgaaacag gatttttatt catctttatc
    12541 ttgatttttg cgttctataa atccttccat acaaaataag attcacagat ttcaactttc
    12601 ctgtctgttt ttagctatta caaatcctta tatgtggtat aacaacaatt tattggcata
    12661 atgaggaaaa acgagttatg atgctttcag agagtaaaga ggtactgttg tcaccatcct
    12721 ttttattgat tacgtacacc actcttagtt ttttcccatt gattacgaaa acaaggtaga
    12781 aacattgatg ttcctacctt gttttctttt tttattttca gatcaaaatc tcaagtagct
    12841 tttgacttga tgtcgggagg ttgtcttcgt tccatcaaac ctgatagaat ctgcgccaaa
    12901 ccatagtttt ctccactatt atttattcct ctaagatagt acccacaata aacgaataaa
    12961 tatttttctc attttcatac tccttctttg aaaatcaatt agatttctct caacccactt
    13021 ttttacaaaa attacgtagc aaaaaagatt gcaagaa
//
LOCUS       DASJON010000069        12944 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJON010000069 DASJON010000000
VERSION     DASJON010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.069"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            171..515
                     /locus_tag="U3989_001449"
     CDS             171..515
                     /locus_tag="U3989_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN1714904.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="U3989_001450"
                     /pseudo
     CDS             516..935
                     /locus_tag="U3989_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="U3989_001451"
     CDS             complement(1027..1377)
                     /locus_tag="U3989_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714905.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="U3989_001452"
     CDS             complement(1343..2674)
                     /locus_tag="U3989_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN1714906.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="U3989_001453"
     CDS             2930..3496
                     /locus_tag="U3989_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1714907.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="U3989_001454"
     tRNA            3641..3728
                     /locus_tag="U3989_001454"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /gene="bsh"
                     /locus_tag="U3989_001455"
     CDS             4062..5036
                     /gene="bsh"
                     /locus_tag="U3989_001455"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1714908.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="U3989_001456"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="U3989_001456"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN1714909.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="U3989_001457"
     CDS             complement(7451..7897)
                     /locus_tag="U3989_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1714910.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="U3989_001458"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="U3989_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1714911.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="U3989_001459"
     CDS             8362..8880
                     /locus_tag="U3989_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1714912.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="U3989_001460"
     CDS             complement(8957..9913)
                     /locus_tag="U3989_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1714913.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="U3989_001461"
     CDS             10079..10885
                     /locus_tag="U3989_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714914.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="U3989_001462"
     CDS             10882..11871
                     /locus_tag="U3989_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1714915.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="U3989_001463"
     CDS             11868..12872
                     /locus_tag="U3989_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1714916.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
BASE COUNT         4164 a         2216 c         2591 g         3973 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaaagat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gaggggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaaaaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attgttcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 catacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcagc caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatat cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt aagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga tcttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaatcga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagt
//
LOCUS       DASJON010000070        12894 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJON010000070 DASJON010000000
VERSION     DASJON010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.070"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(260..979)
                     /locus_tag="U3989_001464"
     CDS             complement(260..979)
                     /locus_tag="U3989_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1714917.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="U3989_001465"
     CDS             complement(1028..2674)
                     /locus_tag="U3989_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1714918.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="U3989_001466"
     CDS             2853..3443
                     /locus_tag="U3989_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1714919.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="U3989_001467"
     CDS             3535..5040
                     /locus_tag="U3989_001467"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN1714920.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="U3989_001468"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="U3989_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1714921.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="U3989_001469"
     CDS             complement(6476..6994)
                     /locus_tag="U3989_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714922.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="U3989_001470"
     CDS             complement(7010..7336)
                     /locus_tag="U3989_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN1714923.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="U3989_001471"
     CDS             complement(7349..9328)
                     /locus_tag="U3989_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1714924.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="U3989_001472"
     CDS             complement(9349..9663)
                     /locus_tag="U3989_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN1714925.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="U3989_001473"
     CDS             9831..10124
                     /locus_tag="U3989_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN1714926.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="U3989_001474"
     CDS             complement(10202..11314)
                     /locus_tag="U3989_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1714927.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11467..11691
                     /locus_tag="U3989_001475"
     CDS             11467..11691
                     /locus_tag="U3989_001475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714928.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            11701..11880
                     /locus_tag="U3989_001476"
     CDS             11701..11880
                     /locus_tag="U3989_001476"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714929.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="U3989_001477"
     CDS             12074..12244
                     /locus_tag="U3989_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1714930.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12894)
                     /locus_tag="U3989_001478"
     CDS             complement(12668..>12894)
                     /locus_tag="U3989_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1714931.1"
                     /translation="VTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKT
                     GETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4075 a         2587 c         2188 g         4044 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actg
//
LOCUS       DASJON010000071        12804 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJON010000071 DASJON010000000
VERSION     DASJON010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.071"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(263..706)
                     /locus_tag="U3989_001479"
     CDS             complement(263..706)
                     /locus_tag="U3989_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1714932.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(924..2264)
                     /locus_tag="U3989_001480"
     CDS             complement(924..2264)
                     /locus_tag="U3989_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1714933.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2533..3429
                     /locus_tag="U3989_001481"
     CDS             2533..3429
                     /locus_tag="U3989_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1714934.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3579..4520)
                     /locus_tag="U3989_001482"
     CDS             complement(3579..4520)
                     /locus_tag="U3989_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1714935.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4646..5767
                     /locus_tag="U3989_001483"
     CDS             4646..5767
                     /locus_tag="U3989_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1714936.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            6025..7527
                     /gene="eat(A)"
                     /locus_tag="U3989_001484"
     CDS             6025..7527
                     /gene="eat(A)"
                     /locus_tag="U3989_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1714937.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7555..8121
                     /locus_tag="U3989_001485"
     CDS             7555..8121
                     /locus_tag="U3989_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1714938.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8332..9393
                     /locus_tag="U3989_001486"
     CDS             8332..9393
                     /locus_tag="U3989_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1714939.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9396..10292
                     /gene="murQ"
                     /locus_tag="U3989_001487"
     CDS             9396..10292
                     /gene="murQ"
                     /locus_tag="U3989_001487"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN1714940.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10306..12288
                     /locus_tag="U3989_001488"
     CDS             10306..12288
                     /locus_tag="U3989_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1714941.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12353..12799)
                     /gene="nrdI"
                     /locus_tag="U3989_001489"
     CDS             complement(12353..12799)
                     /gene="nrdI"
                     /locus_tag="U3989_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN1714942.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         4221 a         2346 c         2653 g         3584 t
ORIGIN      
        1 ccatttgtct ctctaatact caggtcagaa cgctgatttt acaacctctt aataaacggt
       61 gttcaaaagc ttgcatctgc ggtaataagc ctaaccaaac gaaaaatagg agaagctatt
      121 tttgtttggc tgttcttatt acttgatgca gggcagcttt ttcacaaccc cttattcacg
      181 gcgttccaga agtagcttct tcttatttct tgaagctgat aacttctgga acatcctctt
      241 tttttatttg gacggataat ggtcattcca tttctttgat catttttttc attttgtcga
      301 tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca ggatctttta
      361 aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg gccttcatcc
      421 aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg gcaaaaacaa
      481 gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt ttccacatga
      541 acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat actcgtgtac
      601 gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca aacataacta
      661 ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa aacaaactcc
      721 tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa ttattttgca
      781 cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc ggaaaaagga
      841 gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat tccacataaa
      901 taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc attctttttt
      961 tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac cccaatggag
     1021 ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa aaagctgatt
     1081 cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc actgataatg
     1141 atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa taatttgtca
     1201 ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa taaaacagca
     1261 cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc ttttcgatcc
     1321 ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat agatgagaac
     1381 aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt cattttttca
     1441 aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg aaaaagaatc
     1501 aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac acctttgatt
     1561 gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc aatcagttca
     1621 gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt cataccatga
     1681 ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc tgtgcccaac
     1741 atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac actgatttta
     1801 gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag attgatgatc
     1861 gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg gaaccacaac
     1921 tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca catagaccag
     1981 tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat caaaccagat
     2041 aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt catcacgata
     2101 tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc gatcccaatc
     2161 gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc taacatggtg
     2221 atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga cctctttctt
     2281 ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca atgactaatg
     2341 ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat tttaatagaa
     2401 gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa atataggaaa
     2461 cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga cagagaaagt
     2521 gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa atcactttat
     2581 tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact acagtcggaa
     2641 ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt tttagattat
     2701 ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta
     2761 tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa
     2821 ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact tgggctgaaa
     2881 cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc aaagacggat
     2941 ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta taagcaggaa
     3001 tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg gaatgaacaa
     3061 cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg cgggattctg
     3121 gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc catgtatttt
     3181 caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta tcgtcctgaa
     3241 aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct gcatacacca
     3301 tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg ggcaaaacga
     3361 tttatcacta acgattccac tatttatgag caatacgaat atgacctctc ctctgttgaa
     3421 aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa gcacaattct
     3481 tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa aaaaagagaa
     3541 ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc aaggattcct
     3601 tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt
     3661 tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg atggtttttg
     3721 atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa
     3781 gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc tgcgatagaa
     3841 gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc gcctagttca
     3901 gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac acaagctttt
     3961 cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc ttcttgaaag
     4021 acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc tagttcgatt
     4081 tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg
     4141 atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc atgacctaat
     4201 ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat cgaacaaaac
     4261 atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg ttcgttgctt
     4321 tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt accgttaaag
     4381 tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg atcgatcaag
     4441 aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat tgctccggcc
     4501 ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat
     4561 atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta
     4621 cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg
     4681 aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg
     4741 aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta
     4801 cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa
     4861 aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca
     4921 agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag
     4981 ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt
     5041 cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc
     5101 ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga
     5161 tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc
     5221 atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac
     5281 gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg
     5341 ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct
     5401 ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt
     5461 ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca
     5521 aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc
     5581 aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc
     5641 aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca
     5701 aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc
     5761 gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg
     5821 agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt
     5881 gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg
     5941 gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     6001 tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc
     6061 tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg
     6121 aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa
     6181 aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa
     6241 cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag
     6301 atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga
     6361 gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg
     6421 gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga
     6481 aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac
     6541 cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa
     6601 ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa
     6661 atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa
     6721 aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc
     6781 gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg
     6841 aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta
     6901 ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag
     6961 cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt
     7021 cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag
     7081 tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac
     7141 cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg
     7201 ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga
     7261 aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa
     7321 cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat
     7381 gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac
     7441 gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact
     7501 gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt
     7561 aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa
     7621 acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa
     7681 ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg
     7741 aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt
     7801 ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat
     7861 ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc
     7921 ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc
     7981 atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc
     8041 aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca
     8101 gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca
     8161 ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc
     8221 aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga
     8281 tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc
     8341 ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg
     8401 gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg
     8461 gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg
     8521 atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg
     8581 cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag
     8641 attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa
     8701 gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat
     8761 tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg
     8821 ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc
     8881 ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc
     8941 gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca
     9001 gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg
     9061 gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac
     9121 gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca
     9181 gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg
     9241 cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat
     9301 gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg
     9361 aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc
     9421 taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag
     9481 ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga
     9541 caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga
     9601 tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac
     9661 cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa
     9721 tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca
     9781 agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat
     9841 atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt
     9901 gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc
     9961 cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga
    10021 atatgatttc tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg
    10081 atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga
    10141 cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc
    10201 tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg
    10261 aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa
    10321 ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta
    10381 gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta
    10441 aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt
    10501 caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca
    10561 ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa ttcatcagca
    10621 tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa
    10681 caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg
    10741 attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta
    10801 gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc
    10861 ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt
    10921 tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg
    10981 aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt
    11041 gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat
    11101 aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc
    11161 ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc
    11221 ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca
    11281 ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa
    11341 atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga
    11401 caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa
    11461 atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga
    11521 gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg
    11581 gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct
    11641 ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct
    11701 tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa
    11761 gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa
    11821 acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta
    11881 gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa
    11941 ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg
    12001 aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc
    12061 aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg
    12121 acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa
    12181 acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa
    12241 acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac
    12301 aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat
    12361 tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat
    12421 aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact
    12481 gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt
    12541 catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac
    12601 catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa
    12661 aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat
    12721 gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg
    12781 atatatataa aatgttcatg gatc
//
LOCUS       DASJON010000072        12747 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJON010000072 DASJON010000000
VERSION     DASJON010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.072"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U3989_001490"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U3989_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1714943.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U3989_001491"
     CDS             complement(323..892)
                     /locus_tag="U3989_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN1714944.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U3989_001492"
     CDS             1078..1980
                     /locus_tag="U3989_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1714945.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U3989_001493"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U3989_001493"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN1714946.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U3989_001494"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U3989_001494"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN1714947.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U3989_001495"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U3989_001495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN1714948.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="U3989_001496"
     CDS             8554..8739
                     /locus_tag="U3989_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN1714949.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="U3989_001497"
     CDS             complement(8801..9997)
                     /locus_tag="U3989_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1714950.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="U3989_001498"
     CDS             complement(10167..11603)
                     /locus_tag="U3989_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1714951.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="U3989_001499"
     CDS             11896..12705
                     /locus_tag="U3989_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1714952.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DASJON010000073        12681 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJON010000073 DASJON010000000
VERSION     DASJON010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.073"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..211)
                     /locus_tag="U3989_001500"
     CDS             complement(<1..211)
                     /locus_tag="U3989_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1714953.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNV"
     gene            complement(288..2984)
                     /locus_tag="U3989_001501"
     CDS             complement(288..2984)
                     /locus_tag="U3989_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1714954.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(3412..4485)
                     /locus_tag="U3989_001502"
     CDS             complement(3412..4485)
                     /locus_tag="U3989_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1714955.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(4482..5309)
                     /locus_tag="U3989_001503"
     CDS             complement(4482..5309)
                     /locus_tag="U3989_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714956.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(5310..6116)
                     /locus_tag="U3989_001504"
     CDS             complement(5310..6116)
                     /locus_tag="U3989_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714957.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(6128..7213)
                     /locus_tag="U3989_001505"
     CDS             complement(6128..7213)
                     /locus_tag="U3989_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1714958.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(7230..7772)
                     /locus_tag="U3989_001506"
     CDS             complement(7230..7772)
                     /locus_tag="U3989_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1714959.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8030..9019
                     /locus_tag="U3989_001507"
     CDS             8030..9019
                     /locus_tag="U3989_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1714960.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            9016..9753
                     /locus_tag="U3989_001508"
     CDS             9016..9753
                     /locus_tag="U3989_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN1714961.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            9750..10301
                     /gene="coaC"
                     /locus_tag="U3989_001509"
     CDS             9750..10301
                     /gene="coaC"
                     /locus_tag="U3989_001509"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN1714962.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            10317..10949
                     /locus_tag="U3989_001510"
     CDS             10317..10949
                     /locus_tag="U3989_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1714963.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            11034..11681
                     /locus_tag="U3989_001511"
     CDS             11034..11681
                     /locus_tag="U3989_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1714964.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            11686..12669
                     /locus_tag="U3989_001512"
     CDS             11686..12669
                     /locus_tag="U3989_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN1714965.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         3913 a         2608 c         2244 g         3916 t
ORIGIN      
        1 atacattcaa ctcctgttca aaatcacact catccacata agcattaatc accgtaatct
       61 cttgaacctt ccccgtcagt aattgaatac tgctaagaaa gcctgaagct aagtgaccgt
      121 gagtcgctat tagaattttc ttcatatggt taattccttt ctttctgttc acttttatat
      181 aagcaattcc tgtgccaact tttaaaaaca ttggtttaat cacgttaatt cacattaaaa
      241 aaagtgtcta acctaaaaaa aacagattag acactttttt agacacttta tttttgaata
      301 tacggtccta ataattcata gagcagcgaa agctcataag cattgacttt aaaattatat
      361 ttcatctcca ttgattgaaa aatacttcta gaaacataat aaaattcatc ttcatctggt
      421 ttcaaatcat aattcggttc ttcagatttt tctgtccgag ccaagaataa acgttccatc
      481 atcaatgccg tatgcatata gagatttagt tttacacggc catttaaagt gaaatgatag
      541 taattttcat atttagaaat gactgtttcc acttcactca tcacgatcgt tgggttcaga
      601 aagttcaaac ggtccgcaat tccttccaac gaaaagaatt ttactagctc ctgaatcaag
      661 cggttaaatt ctgccggctt catttcagga ctcaaaatat gctccaaatt ggctgctcca
      721 tcaccgtcta agatgtcata aacattaata ttaggaattg aaaaggaacg tggaagatcc
      781 gaagtcgtga tgaccaattt tgtcttgtca aaataagttt catcattttg gtcgattaat
      841 tcacgtagtt ctctgtagtc cttcgtgaaa acttcgatgg attcatccga taaatgatga
      901 cgaaaaacat ctttcaattt gtccgaaatc cccaaacccg acatacagga aatgataatg
      961 ttgctacttt gagaaaagcc ttcaaagtat tgaacattga ttgaatactt gtctgctgca
     1021 tcctccgcaa tctgtttaaa tggcgcattc atcgtcatct tcaagccaat atcaagtgca
     1081 atggaagtcg tcaaattatt cagaagaagt aactcccctt ttaaatcgtt cttgatctgt
     1141 gtgtaaattt gatttaaaga gcccatatct acgatcagca ctagactatc tgtattcact
     1201 tgccgtttca cgaaattcct tgtcgattcg acaatctctg acacattact ctctactggc
     1261 atatccaacg cttcaaaaac ataattgccg cataattgat tcactactgc ttgaatgctg
     1321 ctagccgtac tatctccgtg agcaagcaac aggcccttca gctcgatgga ttcatctaca
     1381 taatcagata aagtcacgac aataagcagt cttagaaagt tctcgtcctt ttctgagagc
     1441 ggtggtaaat gctccaagaa ctgattacat aagtaagtcg tccgaggtag atttgcagct
     1501 aatgtacgat ttatcccggt taagtctgtc ttatagcttg gggaaatgac ataggatctg
     1561 ctaataattt ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc taaaacggat
     1621 acccagacct ttctaaacat ctgaatcatc aattttctac tcattgataa aaaagaatcc
     1681 ttatcaatgt gatgcaccaa atgctgtact tcattcttta tcgaagaaaa attttcattg
     1741 aaattacctt catttatcag ctgagtaatt ttttccaaaa tcgaatcata atcaaacggt
     1801 ttgattcgat ccgtttttat cttttgattt ttctttatca ataaactagc aatcggcaag
     1861 ctttctactt cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt
     1921 aatggttctt cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc
     1981 agcttaccaa tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa
     2041 acaataatat ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct
     2101 aatcgttctc gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc
     2161 cgatcaaagg tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga
     2221 accttagcag aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata
     2281 aatagaaaga gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga
     2341 tagccgttgt tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc
     2401 cccttcttgt atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca
     2461 cagtttaaga tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca
     2521 aaaatttttg tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg
     2581 agtccgttag gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt
     2641 ccgtcatgac caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc
     2701 gcatattcat ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta
     2761 tcttccacta atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc
     2821 gaaattttct cggcagtgac agaagacata ttccctgtta gtcgtatcag ctcatcaaaa
     2881 actcgatctt tagcgctctt tttcattttt tcctccagtt taatgtgtct aaaagcataa
     2941 taaactatcg gctgcaatat gtctattcta tatttgtatt ccaattttta ctttgaaaca
     3001 caaatagtcg aatatttgcc tcagatatct gacaaaataa tttatctacc aatttagttt
     3061 ttatctaata tgatttttat gttttttcat agctacaaat attgaactgg attcatatat
     3121 tagttttatt aatagattac agaagggtta agtatatttg catagtttcc aattacttta
     3181 tccttctgta actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat
     3241 ggttttgtcc acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa
     3301 aagcggcgtt tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat
     3361 ttttagctct ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct
     3421 atacatcttg aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc
     3481 tttgtagact tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga
     3541 aatgtctttt ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc
     3601 tgcattttgt gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc
     3661 tttatttttc acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg
     3721 tgggatcaca taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa
     3781 tgtcactgcc acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat
     3841 tgctttgaca tttggtgtta aatgattcaa tttatttgct gcttctgtca gctgtgccat
     3901 attcttacta ttcaaagaat agcctaaact attcaaagaa agccccataa cttctcgtgc
     3961 accgtcaatc agcatcaaac tgtcttttaa ttccggcttc cacaaatcat cccaatgctt
     4021 aatttgattt gcatcaaaga atttatcgtt atatacgatc cctaaagttc cccaaaaata
     4081 tggaatcgaa tactgattat ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt
     4141 atttaatcct ttgatcttcg aatgatctaa tggaagtact agtttttctt tgatcatttt
     4201 ttggatcata tattcacttg gtatagcgat gtcgtagttt gtcccacctt gctggatttt
     4261 tgtgaacatc gcttcattag aatcaaatgt ttcataattc actttgtatc catattcttt
     4321 ttcaaatttc gtgatcaaac taggatcgat ataatctccc cagttataga tggtaagtac
     4381 ctttgctcca ctcatgccac ttgttttttc aagttgacga acaccaagca gcagcaccgc
     4441 aataatgatg agaataccta aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc
     4501 ccctcgcgta ctcttctgtt tttcttcagc cgttttggac ggttttcttt actaataaag
     4561 tagtatccaa tgaccaatat catggagaac aagaaaacta acgcacttag cgcattgatt
     4621 tctaaactga ttccttgtct tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca
     4681 tttcctgtga cgaaaaatgt aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa
     4741 tatccagcta tgattcctgg agaaagataa ggcaaaatga tatttttgac tacttggaaa
     4801 ttattagcac caaggtctct agccgcatcg accattgaat cgttcatttc ctgcaatttt
     4861 ggcaagacca tcaagacgac gattggaatg ctgaaagcaa tatgggaaag caatacactc
     4921 tgaaaaccta atccgaatcc tagtagtgta aagaaaatca agaaactagc accgataata
     4981 acatcaggag agactaataa gatgttattc atgctcaaca acgctgtccg tgcacgtcgt
     5041 tttttcgtgt aatagattcc catagcccca aatgtcccga tgatcgtggc gatcaatgca
     5101 gataagaaag ccagcatgaa cgtattcaat acgataatga tcaaacgtgt atcttcaaaa
     5161 acagcttcat aattttccca agtaaatcct gtaaagctgt tcatcgttcc tccttcattg
     5221 aaggaataaa aaatcagata gaagatcggt gcatataaga ataaaaagac gatccctaaa
     5281 taaagatacg accatttaaa ttttttcatt tatgagcgac ctcctttttt cttctcgcct
     5341 gtcaggagca tcacgatgaa catagcaatg attaaaatca caccgattgt agaacccatc
     5401 ccccagtttt gtgtaaccaa gaaatgctgt tcgatagccg ttcctaaagt gatcacccga
     5461 tttccgccaa tcagacgagt cagcatgaat aaagaaagag aaggaataaa tactgcttgg
     5521 acaccacttt tcacaccatt taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag
     5581 ctattcgcgc ctaaatctct actagcactg atcaaagaag gattcatctc ttccaatgca
     5641 ttgaaaattg gcataatcat aaatggaatc tcgatatacg ttgccacgaa cagaaaactg
     5701 aagtctgtga ataatatctg tttcggcccg atcccgataa aatcaaagaa atgattcacg
     5761 ctgccgtgca cactgaaaat cccaataaat gcgtaagctt taagcagcag attcacccat
     5821 gtaggcaaaa taatcagcat cagccataat tgcttgtgct tcagttttgt taaaaaataa
     5881 gcagtcgggt aactgatgat cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa
     5941 ttgatcgtca tagaaagcga tgtccccgac gtaaaataag atagataatt attcagcgtg
     6001 aattgcccat tcatatcaaa aaaagattga taaatgatca ttaatacagg cgcgatcacg
     6061 aataaaccga gccacaaaat ataagggacg gaatagatcc tgcgcattgt tttcattctt
     6121 gtccccccta ttcttcgtag ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga
     6181 aacgcatgac atggatatct tctggctcaa aggccaatcc tactttgctt ccttctttag
     6241 cttttttcgt tgaatggacc atccattcat tttgctgttc atcataacag atgatttcat
     6301 aatggacacc acgaaacagc tgagtatcga ccgttacgac tagttttcct ttatctggtg
     6361 tcgtgatcgt caaatcctct gggcgtaaaa caacttcaac tggttcattt agacgcatcc
     6421 cgccatcgac acattcaaat tgcttaccaa caaactcaac tagattatct tcgatcatta
     6481 caccgtccac gatattgctc tccccaacaa aatcagcaac gaaatgattg attggctcat
     6541 cataaatatc tacgggtgta ccactttgga cgattttccc tttattcatg acaaaaatct
     6601 catcactcat tgccaacgct tcttcttggt catgagtaac aaaaataaac gtgattccta
     6661 atcgttgttg cagatcccgc agttcatact gcatatctgt acgtagtttc aaatcaagcg
     6721 ccgataacgg ttcatctaat agcaatactt tcggttcatt tacaattgct cgtgcaatcg
     6781 ctacacgctg tctttgacca ccagacattt cactaatctc ccgtttttcg tatccaggaa
     6841 gctggaccat acgcaaagca tcttttacct tcttttcgat atctgatttg gacattttct
     6901 tgatcttcaa tccaaaagca acgttatcaa aaacattcat atgaggaaaa agtgcataat
     6961 cttggaagac tgtatttact tgtcgcttat tagctggcaa atcattcaac cgtttccctt
     7021 caaaatagat gtccccttct gtagcttcgg tgaatccagc tataatacgt aggatcgttg
     7081 ttttaccaca gccggaagga ccaagcagtg tataaaattt tccttgttcg atctcaaaac
     7141 ttactttttt taatactgtt tcatcatcat agcgtttcac tacttgatca aatgaaatga
     7201 ttggtttccc cacgttttta ccccctatat tacaaatatg attgcgttgc aacaatcaat
     7261 acttttgttc gttttttgag atgattcctt aattgatgtt gttttgtcgc ttgataatag
     7321 atcgactgtc ctgcttttgc cacatagacc gtctctccta agcttaactc taattcccct
     7381 tctaatacaa aaataaacgt ttcagataat gaaggttcaa acgtcttata ttctcctgca
     7441 ccttcaaaag tcagcaagac aggctccatt tctttttcat tcgaatcagc cacgagccat
     7501 tccaattcat acccgttttc ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc
     7561 acttgcagat tcgctgcttc ttgatgaaaa aattcttctg gagtcacacc taagacttca
     7621 agaatagaaa agaatgtttc cattgatggt gaacttaaat ctcgttccaa ctgggaaata
     7681 tacccttttg acaaatctgt gcgttcgcct agttcttctt gtgtcaaatt cttttggatg
     7741 cgcaaatttc ttaacttctc accaatttcc ataatttcct ccgaatcgaa gttttctaat
     7801 agtaaacttc ctgtttacta ccactcgtat tagtatacga aatttattga aaaaggcaac
     7861 cctttgtaaa attttttttg ctgaatattt ctttatcttt tggaataaat tctagttgtt
     7921 tgattcttta tttttcacct aatttttgtc taaatttacg gaagaacaag aataaaagag
     7981 taggctttat ggtatgataa aagaaaattt taaaaggagt gtctcacgaa tgaacgcttt
     8041 tcgatctttt caggtaataa aggaacctgt tttccataca gcaatcagtg atctgccatt
     8101 tactgagttg gctgaaaatg aagtcttggt aaaaattgcc tattctgatg tcaattataa
     8161 agatgcattg gcaatgagcg agtctggtca ggtgatacga acatatccga tgactccagg
     8221 gattgattta tccggaactg tcgtccaaag taacaatcca cgtttttcaa aagaagatct
     8281 tgtacttgct actggttttg ggcttggcgt cacccatcca ggcggttata gccaatatca
     8341 aaaagtacca ggagactggt tagttcctct accaaaaaat atgacgctgc gtcaagcgat
     8401 gatccttggt acagcagggt ttactgctat gttgtgtgtg aacgctttaa tccgtcaagg
     8461 tatgacctcc gataaaaaag ttgtcgttac tggtgcttct ggcggtgtag gcagtacagc
     8521 gatagccctg ttacataaac taggattcca gtcaatcatt accttttcca gaaaagaaga
     8581 gtctgtcaca tggctaaaaa gtctaggtgc tttgcaagtc gtacgacctg atgaatttct
     8641 gccagagaca acaaaagctt taggaaaaca gcaaatcgac tatgtcatcg acacagtagg
     8701 tggcgagcag ctaagcagtc ttcttccatt gatctcttat aacggtgcag tagctttatg
     8761 tggcaatgct gggggaatca aactaaacgc gactgtcttg ccttttattc tgcgaaacat
     8821 ccagttgatt ggaattgatt ctgtcaatgt accaatagat caacgtcttt ctttatggca
     8881 acagatggcg gatttgcaga ttgcagatga actggtcgtc caagagatta ctctggatca
     8941 attgcctgaa acagcatcta aactacttgc tggaacacac caaggaagaa cactagtaaa
     9001 tgtaggtgac cataaatgaa gattctaatc acagctggag gaacgactga aaaaatcgat
     9061 caagtacgtg ctattacaaa tcattcgaca ggacgattag ggcaagcatt agctgattat
     9121 ttagctgcca atccggatac cacaatcgat tacgtgacta cgaaacaagc actgaaaccg
     9181 aaaagacact ccaatataac gatttatacg atcgaatctg cactggactt atttttacaa
     9241 ttagaagaac taacgaaaaa agaacactac gatgcgatta tccacagtat ggcagtcagt
     9301 gattttactc ctgcttttag tttttcagaa gaacagctgg caaaaaacct acccacttct
     9361 tcaacgcaag aagaactgga caattggttt gcggaaaatg aacagacaga taccactgtt
     9421 tcaaagatct cttctaacac cgagcatctt gtgctcgttt tgaagaaaac accaaaaatc
     9481 atttcttatc tacgtgaatg gcagccaaaa gcaaaaatca ttggtttcaa actattagta
     9541 gatgtaccaa aggaatctct tctggcagtc gcaaaaaata gtctgataaa taacaaaact
     9601 gattttattt tcgcaaatga tttgacggaa atccatggcg agacacacca cggctatctc
     9661 ttatctaaag atggaacagt ggaagaagct caaagtaaat ctgagattgc tgcattgatt
     9721 actgagaaaa ttagactgga ggaatcaaaa tgaaaaagat cttactaggc gttactggaa
     9781 gtatttctgc ttacaaaagc gctgatctta caagtcaatt cgtcaaatta ggtgcccagg
     9841 tagatgtgat tatgtctaaa agcagcacac attttatcac gccattaacc ttgcaatctc
     9901 tgtcgaagcg cgcagtccat acagacgtca tgcaagaatc aaatccgcag gtaatcaatc
     9961 atatcgaact agcaaaacaa gctgacttgt ttctcatcgc gcctgctaca gcaaacatta
    10021 tcggcaagct agcaaacggc attgcagacg atcttctttc aacagtcgca atggcgcttc
    10081 cagtgggaac accaaaattg attgcccctg ccatgaacac gaatatgtat caaaacccga
    10141 tcactcagcg taatctggaa acattgagat ctgtcggcta tcaggaaatt gaaccaagag
    10201 aatctctttt agcttgtgga gattttggaa aaggtgcctt agctgatatt cagacgatca
    10261 tcgaacaggt catgctgact cttaatgaaa gaaatgctta gaaaaaagga gtacttatga
    10321 aaaatacaaa aaactttaca ttaactgcga tgtttttagc tattttgatt ttattagctg
    10381 ttacgccttt aggttttatc cctatcggtc caatcaatgc aacaaccatg catattcctg
    10441 taatcattgc ttcgatcgtt ttaggaccaa gactaggtgc ttttcttggc ggtacatttg
    10501 gactgatcag catgatccgt agcacagtta tccaaacacc tttgtctttt gtcttttctc
    10561 cgtttatccc agtcatcgga acagaacatg gaagctggaa agccttgctg atcgcgttta
    10621 ttcctcgcat cttgattggg gtggttcctt atttcgttta taaaggtctg cgcaaactaa
    10681 tgaaacaaaa agcagattcc gtttctttat tcttagctgg actttccggt tcattgacca
    10741 atacgatttt agtgatgaat atgatctact ttttattcca gcaagattat gccaaagtca
    10801 tcggaacaaa tttaaatgct gtttattcag ctattctagc ggttattttt acaagtggta
    10861 tacctgaagg aatcgtcgca ggtcttgcga ctgcagcagt atgcaatgtc ttgttaagat
    10921 acttcaagca cccttctctt actaactgat agtcatcgta gccgatctct gatttttttc
    10981 tcttttggtg ttactattga atggaaagat ttgacgtcga aaggatgatc caaatgaaaa
    11041 tttttgttgt aggtgcaaac ggacaaattg gccgacacct gataaaagat ttagctccaa
    11101 gttcccatga aatttttgca ggggtgagag atgttgcttc ccaaacatta gtaaaaaaag
    11161 agaatgtttc gtatgtctct tttgatttaa cttggtccgt ggaaaaaatg accgaagcat
    11221 tcaaaggcat cgatgtattg atttttgctg ccggctctca aggaaagaat cttctgcaag
    11281 tagacttgga tggcgcaatc aaaacagtga tcgcagctga aaatgcgcat gtctctcgct
    11341 atctgatggt cagtgctgtt tatgcagatg aaccagcaaa atggcctgag tccatgacag
    11401 attattatat tactaaacat tatgcagatg aatggctgaa acgaactaac ttagattttg
    11461 tcattcttca accagtcaca ttgactaacg atgaagaagt tacttccatt caattaacta
    11521 agcctaatga gaaggcatca aaaacgatca ctcgctctac agttgctgct gtacttgccg
    11581 ctctagtgga agaaacggat atttctcgta cgactctagt actttctgaa ggtagcaaag
    11641 agttgaatac agcattccaa gaatgggcaa aggaggaata gatccatgcg tgccatttca
    11701 atcaagcatc ctggttctgt agatgtatta gaacaaattg aagtgcctgt tcctaaacca
    11761 aaacaaggcg aattactgat caaagtccat acagcagctg tcaatcgaac agatattatg
    11821 aaaagagaat cagtctcttt gaaacctccc taccctattt tgggagtcga agtagctgga
    11881 gaggtagttg agaataaaag tgatatctcc acttttactc ctgggacaaa agtatacgga
    11941 ttagtcaatc taggtggtta tgccgaatat gctgtgatgc ctgccgatcg tgcaatcctt
    12001 ctccctgata cgctagacta tgtttctgct gctggaatct cagaagtttt tttgactgct
    12061 tatcaaacac tttactggct tggaaaatta caagaaggcg aaactgtctt gatccatgca
    12121 ggagcaagtg gtgtgggaac agcagcgatc caattagcta gacagctttc tcacgcgaaa
    12181 atcatcgtta ctgcaggatc agaagaaaaa ctcgcctttt gtaaagaact tggtgcagat
    12241 gaattgatca actataaaaa acaagacttt caagaagagg tacgaaaaat cactgaaggt
    12301 cggggagtcg atgttatttt ggatttcatc ggcgcttctt actgggaaaa gaatttagct
    12361 tctatagccg ttgatggacg ttgggtcttg attggtatgc taggtgggac catcgtgcct
    12421 gagatagacc ttggtgtatt gattgggaaa cgtgtccaac tgcttggtac attattgaca
    12481 cctagaagtg atcagtataa agcagagctc gcacaaactt ttatgcaaaa agctgggcct
    12541 tttctagaaa atggcaagat tcggccaatc attgaccgta cattccaatt cagcgcagtc
    12601 aaacaagcgc atgaatatat ggaagcaaac aaaaacatcg gaaaaatcat tttgaagata
    12661 actgaataaa atcatgctga t
//
LOCUS       DASJON010000074        12635 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJON010000074 DASJON010000000
VERSION     DASJON010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.074"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(146..1111)
                     /locus_tag="U3989_001513"
     CDS             complement(146..1111)
                     /locus_tag="U3989_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN1714966.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="U3989_001514"
     CDS             1345..2190
                     /locus_tag="U3989_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1714967.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="U3989_001515"
     CDS             2381..3568
                     /locus_tag="U3989_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1714968.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="U3989_001516"
     CDS             3574..3930
                     /locus_tag="U3989_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN1714969.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="U3989_001517"
     CDS             3932..4270
                     /locus_tag="U3989_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN1714970.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="U3989_001518"
     CDS             4665..5699
                     /locus_tag="U3989_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1714971.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="U3989_001519"
     CDS             5692..6378
                     /locus_tag="U3989_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1714972.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="U3989_001520"
     CDS             6402..7250
                     /locus_tag="U3989_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1714973.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="U3989_001521"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="U3989_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN1714974.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="U3989_001522"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="U3989_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN1714975.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="U3989_001523"
     CDS             9516..10751
                     /locus_tag="U3989_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1714976.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="U3989_001524"
     CDS             10738..11208
                     /locus_tag="U3989_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN1714977.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="U3989_001525"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="U3989_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN1714978.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4179 a         2366 c         2798 g         3292 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atata
//
LOCUS       DASJON010000075        12604 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJON010000075 DASJON010000000
VERSION     DASJON010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.075"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..241
                     /locus_tag="U3989_001526"
     CDS             <1..241
                     /locus_tag="U3989_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714979.1"
                     /translation="MRECIKFYEKAEKADLTDLEAIDSMIAITADIFQDPAVTFDAIL
                     DGLTASELVPALESVFEQINELGNNEKKQTASKKK"
     gene            265..456
                     /locus_tag="U3989_001527"
     CDS             265..456
                     /locus_tag="U3989_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714980.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            456..4304
                     /locus_tag="U3989_001528"
     CDS             456..4304
                     /locus_tag="U3989_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN1714981.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            4305..5177
                     /locus_tag="U3989_001529"
     CDS             4305..5177
                     /locus_tag="U3989_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEN1714982.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            5174..6223
                     /locus_tag="U3989_001530"
     CDS             5174..6223
                     /locus_tag="U3989_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714983.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            6227..7051
                     /locus_tag="U3989_001531"
     CDS             6227..7051
                     /locus_tag="U3989_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714984.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            7069..9129
                     /locus_tag="U3989_001532"
     CDS             7069..9129
                     /locus_tag="U3989_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN1714985.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            9146..9295
                     /locus_tag="U3989_001533"
     CDS             9146..9295
                     /locus_tag="U3989_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714986.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            9292..9738
                     /locus_tag="U3989_001534"
     CDS             9292..9738
                     /locus_tag="U3989_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714987.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            9740..9877
                     /locus_tag="U3989_001535"
     CDS             9740..9877
                     /locus_tag="U3989_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1714988.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            9912..10154
                     /locus_tag="U3989_001536"
     CDS             9912..10154
                     /locus_tag="U3989_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HEN1714989.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            10170..10367
                     /locus_tag="U3989_001537"
     CDS             10170..10367
                     /locus_tag="U3989_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1714990.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            10378..11394
                     /locus_tag="U3989_001538"
     CDS             10378..11394
                     /locus_tag="U3989_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1714991.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            11509..>12604
                     /locus_tag="U3989_001539"
     CDS             11509..>12604
                     /locus_tag="U3989_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714992.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVL"
BASE COUNT         4473 a         2042 c         2723 g         3366 t
ORIGIN      
        1 aatgcgtgag tgtatcaaat tttacgagaa agcggaaaaa gcagacctaa ctgatttaga
       61 agcgattgat tcaatgattg caattacagc agatattttc caagatccag cagttacatt
      121 tgatgctatt ttagacggtt tgactgcgag cgagttagta ccggcattag aaagtgtttt
      181 tgaacaaata aatgaactgg gaaacaatga aaaaaagcag acggcgagca aaaagaaata
      241 agtttttctg aagctaggaa agcaatggat caaatctaca aagatttaat cgaagcaggt
      301 tggacgatga gagatgtgga cgaagccgac tatcattatt tgttacacct ttttggagaa
      361 gtggagagtg gcgaagaata tgtagatggt gctgatttca tcaaacaatt tttatcggct
      421 gaagacttag taaaacttga ggaaggaggt aaataatggc aggaaaagga caaccggcag
      481 gaaatatcaa gctagggatt agtttagata gcactagttt tggtaacacg ctggacgaaa
      541 tcaatgcgaa agtcaaacaa gctgagtcga atatgcgtgc caatctaaag gcttatgatt
      601 cagcaggacg ttcatacgaa gcacttagtc aaaagacgaa agacttgtct acggttatgg
      661 aagggcaaaa cgccaaagta agagaattaa caaagcgccg tgatgaagcg attagcaagt
      721 atggcgagga atcgaaacaa gttgctaacc ttaacacaca gataaacaat gctaccgcaa
      781 aatataatgc ttacagtcgc cagttgaacg acacaaaaaa agaattggtg tattccaaaa
      841 cagccgtcaa tgatttatct aatgaaatca aagaaaatga acgacaaatg aacgccgaag
      901 taaaagcgtt gaaagccgct ggtgatgaat ctggtgcttt tgaagcaaaa caaaaagggc
      961 tagccaaaca aacggaatta tccgagaaag ctatcgaaga acagcgcaaa gttgtgaaac
     1021 tgatggctga tgagtttggc gattcagcaa atgaaaccga agatgcaaaa agggcattag
     1081 aaaagttaga acgacaaagc caaatatcta gcaggcaatt agaagcactc aaaagctcca
     1141 gcgatcaatc aggaaaagaa atagaagatt ttggcgacaa gtccacaagg tcagctagga
     1201 aactggacgg actaaaagac aaattaagct cgctaaaaag cgcattttcg tttggtgcag
     1261 ttgctggatt agcgcataac gctattagca gtgtagtaag tggcgtgcaa ggtttggttg
     1321 gcgaagcagt aaacgcatcg gattcattga tgaagttttc caaaaccatg gagtttgcta
     1381 actttgggaa gtcacagata gaaagctcga aaaaagaaat gaaagactac gccgataaga
     1441 cggtttatgg tttagaagaa attctgaaca caaccgcaca attggcatct aatgggattc
     1501 ctaactatac agaactaacc aaggcggcag gtaacttgaa tgccgttgca ggcggttcta
     1561 gtgatacatt caaatccgtt gccatgatgc taacgcagac ggcaggagct gggaaactaa
     1621 caactgaaaa ctggaatcaa ttagcagatg cgataccggg tgcttcagga ttgttacaag
     1681 acgctatgtt gaaaaacgga gcttatacag gaaacttccg tgatgcaatg gcgcaaggtc
     1741 aaatcacttc cgacgagttc aaccaagcaa ttgtacagtt aggtatgaat gacggagcag
     1801 ttaaggcagc cacttctaca gacacattga gcggttcttg ggagcagatg aaatccactg
     1861 taataaatgg gctacaaagt attatagaaa aaataggcgt tgaaaatatc actggtttca
     1921 tcaacagagt aacaaaaggg attgaaaatt ctattcctaa aattactcaa tttataggtt
     1981 ggttgagaga tattggaacg tggatcgttg aaaatagaga gccactaaca tggattgtcg
     2041 gaatcatagg cggaattaca ttagcagtaa aagcattgaa cgtagcaagt atgttgctgg
     2101 caattactgg cggaacattg gcagcccctt ttgtggcgat tggtgtagca ttaggcgcac
     2161 tagcaggtgc tttggtgata gcttatacaa aatctgaaac atttagaaat atagtcaatg
     2221 cggcttttac agctgtgaaa aacgtagtta tgagcgttat caataatttg gtggaatact
     2281 acaaaatgtt gtggagcgtg ttgcagtggc tttgggaaaa aataaaagaa tgggcttcat
     2341 ggattggcaa taaattcatt gaaatgaaga acagcgttgt gaatacggtt cagaacctat
     2401 ggaatggcgt gaaaaacttc ttcagcaatg gcgttggaga tacgtggaat aaggtagtcg
     2461 gttgggtaaa aaacattttc aacaaagcaa ctgaattgaa gaacaacgtt tctgatgtaa
     2521 tcggtaacct gtggaatggt atcaaagaca cattccgtag aggtatcgat acggtattca
     2581 attggttttc agaactacca acgaagatga agaatgccat tattggcggt aaaaacgcca
     2641 ttgttgatgc gttcaaaagt attttcaacg cagcacttaa agcgataggt aaaccagtta
     2701 acgcaatcat ccatggagct tcatgggtac tagaaaaatt gggcgcagaa cctctacaag
     2761 aatgggatgt accacaatac gctacaggta caccagcagg tgggcaccca atcaatggtc
     2821 caatgatggt taatgatgga cgtggagcag aaacagttat cacaccagat ggtagagcct
     2881 tcattcctaa aggacgcaat gttgttctaa acgcaccaaa aggaacgcat gtcttgacag
     2941 cagaagagac cgcgcaactt caaggttcta aagctcctaa gtatcgttac aaaaaaggta
     3001 ctaacttctt tggtaacatg tgggatagtg tgaaaaaggt tgctggtaat gtaggtcaca
     3061 caattaaaaa cgtagtaggt gacgtgtggg actttatttc agacccggga gcgttagcta
     3121 gaaaagtact tggggggtta gatgtattag gtggattgac aaagtatcca ttagaagtag
     3181 gtaaaggcat cctatctaaa gcaacaagtg cactgactga aaaaatcact gggttgttct
     3241 catctggtaa cttagatacc tccgtaggaa caaatggcgt ctataaatat ttggctgatg
     3301 ttgctaagtc tgtgatgaag aaattcccag gatttgtggc aactagtggg tatagaccag
     3361 gtgaccctta ttcacatggt aaacgtaatg ccattgatat tgcattacca ggcgtcacag
     3421 gaggctcacc tagatacaca gaagcagcaa actacgcttt tgacaaattc gcttccaaaa
     3481 ttggttacgt aatcactaat gggaaagttc gtgaccgttc aggacaatca ggtcaaccag
     3541 caactggtgc atgggagcca tggcccgatg gagatcacta tgatcatgtg catttaaacg
     3601 gtgtgaaaga cccacaaaac actcaaattt caggagatag cgtgggaggc agtggggtag
     3661 aaagatggcg caatgtagca attagagcgt tgaaaatgac cggtcaatac agtactgcaa
     3721 acttaaatgc attactaaat caaatgcgta cagagtcaaa tggtaatcct aatgcagtta
     3781 acaattggga tattaacgcc aaaaatggaa caccatcaaa agggttgctc caagtgattg
     3841 acccaacatt cagacagtat gcaatgccag gattcaacag caatattttt gacccactat
     3901 ctaacatctt agcttcaatc agatacgcac tatcaagata tggctcacta acaaatgcct
     3961 atcgtggagt tggttacgca aacggtggaa ttgtaaacca acatcaaatt gcggaaatcg
     4021 cagaaggaaa caagccagaa attattattc cgttagataa ggctaaacga tcaagagcga
     4081 tgcaattatt gtctatcgca atggacaaac taggagtttc tcctaaaact tatggaaatg
     4141 cgacaactgt ttccagtgat tacgaaatcc tagaagccat tgaaaaacag gcggcaacaa
     4201 cgaatcagct gttatcgtta ttgctggcat ttttcaaagg aaatagccga actgatagag
     4261 atttagcttt agatattcag aagatcttgg ttaggaggat gtagatgcga actgtattac
     4321 tgagaaatag aataaacgaa gaaattgatt tgtccacaga agactatttt gctactgggt
     4381 tgagtaatat ggggtttgaa gtaaaaaaag aacatgtggg acagtggggg aatttcagag
     4441 aaagcagtga gagcgttgaa atatctgaat ttcagtcatc tgtaatcatt tctgtgcatg
     4501 ggtttcgaga aaaagaactg tacaattcac ttgtgcagtt cctatcggaa ggtccgtttg
     4561 aactggaatt tgcttttgac ggcgaaacaa tggtaagaag atgcagtcta aaatctttaa
     4621 gtaagactga aatcgatccg aaaacatcgt tactaacaga cactttagaa ctatatttca
     4681 catcaaactg gtattccgta aaacgcgaaa aattaattca gcgaccgaat gtagtgaaaa
     4741 cacgtggtaa ggtttttccg tataaaagat cttatatcta cactcaaaac ttgtgggaga
     4801 aaaaaggtgt attcaaattc aataataatt ctgtatacct aacgaatagc aaggaacgga
     4861 tgtccccgct aaaaattcgt gtgattggga agtgttcaaa tccgtattgg gaagtaatcc
     4921 aaaactcaca aatcatcgca acggatggat acttcataga tatgactgaa acacagactc
     4981 tagaagtatc aagccttttt gaagatacaa cggcgatttt aaaagatatt gcaggggtag
     5041 aatcttctgt ctaccaacaa caggattata caaaaactaa ttttgttcag gccccaactg
     5101 gagaattcag cattgtgttt catgttgggg gggcagacgt agagattgag ttatacgagg
     5161 agcgtgatct gttttgattt tagcagtcac gctttttcat cgtgatttaa tgctttataa
     5221 cgaacattta ttctctaaca gttttgaatt tggtgtggac gagatcaacg aagaggctag
     5281 tagctttacg atggataagt acgttcctgt aaaaacaggc gattttctac tagcaaaata
     5341 tattcctagt ggaaaatttg catattttgg tgtaattacg tcgcaggaag acgaaaaaat
     5401 cagttgtaaa agcttactta gtttagctga tagtgagata ccgactgcac gtgtgtcagg
     5461 agataactac gaagagcata tccggcggtt gattgaatac tatttgctga atgatccaac
     5521 gaaacaacta aaagatattt tagacgtcaa agcagaaagt gcgacctctc attcgtatca
     5581 agctaccgat acgaataaac acaagttaag tgcgtatatt ctcaatggtt tcaaaaaata
     5641 caacgtaaaa tggtatttca agggaattca aaacagaaaa atttatacag gcatacgtgc
     5701 tgtaaatgaa tcaatttaca ttaaagacaa ttcttctgaa tttagcgatt gggatgtgtt
     5761 tgttcaggcg ccgggcgctg gaaacgagaa caagctatta atcgttgata aagcaatgaa
     5821 agatatagag aaaccgataa tactgtcaac atggtatttg gacgaagaaa acaatttgac
     5881 acaagatgga tcgaaagaaa atatcacgaa accaactgtc aatttggtta atatctacga
     5941 tcaaaccgca gaagataaag catcctacga agatgtggca aaatcagagt tgaaaggcaa
     6001 tacgtattcg catgaaatca aagtgaacgt tgtaagaaat gcaaaaaatt tgaatgtcga
     6061 aacgattgaa acagggatgt ttgccacgat ttcttataaa ggaaagatat acaagtcggt
     6121 tttgacagct tggcgaatat caagtgataa ggagtttgtg gaattgactt tcggaaacat
     6181 aagaagtcgt tttatggatt attttgaaga taatggggga taaaaaatgg ttagcaatgt
     6241 ggatggatat caatttgaaa acgtgaaagt aagcgcagaa aatgatgcta gactttatca
     6301 cgttttatat aatcggaaaa atcaggttat tgatggttac gatcagtcta tgaatttatc
     6361 ttcaagcgga ttaacagtaa aagttgctgc aggagcagcg attattcaag gtcgtatggt
     6421 cgttgttcga caagaagaaa gtataacagt tccagcaaac tcaagtggtt atatagcatt
     6481 aacagtggat ttgacacaag aagttatacc tggatctatt cttccagaat cggaagaata
     6541 tgaatggact aataatcaag tcaagctaga atttataaca aaagttataa aaggtaattt
     6601 aaacaatggt gataaggtct ataatttacc actatgctca gttaattcta ctggatcaac
     6661 tgtttcaatc tcaaagatat cggatagtta cgagctgact ctctctaaag gagaaatttt
     6721 gtggaggggg actgcgttaa tgcatgatac tcaaactgtc caaccttcaa aaaaaatttg
     6781 gcagacagtt tcaggttttt tgttagtatg gctcccatac gaaaacgggc aagcaattga
     6841 ggatagatat gtaactacgc ctttttataa ggagcgtgta acttttacta atgttttagg
     6901 agaaattgtt tcaggatttg atgactatca caaaaagtgg tttagtaaac gaataaacta
     6961 taattcgaat actaatatat ttacaggtgc agcaagcaat gcaagtggag ataacgcaaa
     7021 tatggtgctt aggtatatag tttcttttta gttaggaggt atggaagagt ggcaaattta
     7081 gaaattaaat tatctgcaaa taagagacag ccttatctac gtcaccgtgt tgttggtaga
     7141 gttggtgatg gggggctcac aacaatcaat gtacaacttc ttgaggaaga tgaaattaca
     7201 ccttttgtaa ttaatctaaa cggtactttg aaatttgtgg gcgaagtttc aaacggtaac
     7261 tataccgagg gagaaccaga aataatcgat tcgactaatg ggttaattag ttacacgttc
     7321 actaagtcaa atttcagcac gagtcatcaa ttcaaacaag catattttga atatgtagat
     7381 cctaacggca aaaaagtaac ttttcagaac ttcatcatag acgtgttaga acgagtggat
     7441 attaattcgg agcaagcgaa atactatatt tcttcattgg aaaaattaca gagtgaaatg
     7501 caaaccactt tcaatcagtt cattagtgat aaacaggtcc aatacgatca aatctactcg
     7561 aaatataacg aattagtaag attgataaat gaatcagata agcaagtaaa tgatcgtatt
     7621 gatcaaacca atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc
     7681 atcgactttg ggggctatga ttattcgggg aatccgaatt tgttaagtaa gctatcatac
     7741 gacttaattg aaaatcaaaa tacttcagct ggaacacttt ctaagggtga aaactcgttt
     7801 aaatataata agatatcagc tgaagtggaa ggtggagtag agttatatta taaacgaaga
     7861 ggtatagcta actggttacc ctctaataaa acgcttgtaa tgactgttaa gcttagagct
     7921 ggagcggact atagtccagt tgacggaaaa cttatactga ttagatatag gtatgttgac
     7981 agtggaactg gcaagattgt tttagactta cctattaaca gtaattcgat aactcaggaa
     8041 tggaaagagt ttagtattac tggaactact ccaacattta gcccacaagc ataccatcct
     8101 tggatacaat ttagggctca agatggggta cttggggaaa tagaaatgag ctatgacatc
     8161 aaaatcgaag aaggctctac agctacacca tatcagccta acttacttga tgactcttac
     8221 tggctaggta aaacaccgtt gggtgagaat ttgataacga atgccaagtt cccaattata
     8281 acacgaagca atcctatttc tggttttgat atttcagaag aattaataat aggtgaaact
     8341 tatacggtat ctttaaaagg gactaagcca gctactaaag aattccactt atactacgga
     8401 gaagcaaatt accagcagtt gcagtatcag gcaactttac ttcctgtgga aggattaaca
     8461 gatgtgtgga gtgccacttt tacagccaga aatactagtg aaacgacaaa tttacttaga
     8521 gtagcgttat ggcaaaaacc taattctgca acatatgata ctgttcaaat tgactggctc
     8581 aagattgaaa aaggcgacac acgcacacca aatatcagtc agtttaaata ctttggtgaa
     8641 ggcttgaaag acagtaacaa tccgaacgac tacagctggg atgtcacacc agagtatact
     8701 gaaaaaagct tgaataatac ggttagtctg actgaaccgc aatccatcga aggattgaaa
     8761 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
     8821 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
     8881 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
     8941 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
     9001 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
     9061 gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc aattacttac
     9121 acgatttaga aaggcgtgaa tcgatatgaa ggcagcatat agaccaattg aaccttacgg
     9181 attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca cagaagtcga
     9241 accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata aatgaaaaac
     9301 atttggaaat atggccgtac tggcggagag tatgcaggaa aagtattgga cgacatgctt
     9361 gtatccgttc cttacacaga tcagccaccg ctcgaagggg ttcgttcaga tggcgaaccg
     9421 ctaacgattg ctgatcagat gtttgatcct aaattgaacc aatggattat tttagcgaac
     9481 gcgttagatc acaacgattt aaacaatctc aaagcgatgt acgaggctct tgaacatgaa
     9541 aacggcaacc taaaacagct aaatgccaaa ctcatgctaa gcgatgtagc gattaaacag
     9601 gaaaatactg cattgaaaga aaaagctgac agtttagcac aaatcaattc aaaaatgatg
     9661 cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct
     9721 tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg
     9781 ctgttttact gacgatcaag ttcgtctatt cgttccacta tgtatcacag atgaagaagc
     9841 agatagaatt attagcaaag aagagagcgc atcttaagtg atgcgttttt ttgttggaaa
     9901 gttggtggaa catgaaagaa gaagcgctcc aagacgttgt ggagagatta gtaagaattg
     9961 aaacaaaatt agacaactac gaatcactta gagaaaaagc tgatagtgca aaagatttgg
    10021 cagacaaagc ctactcagta gcgctaaaca acgcagaaga tatcaaagaa atgaaagcca
    10081 ataataaatg gtcgtggggt tacatgatcg gtttaggcat tacgatcatt ggctatttct
    10141 tgactaaatt gtaaaggagt taagaagaaa tgattttacc cgataagtat tatcaagtca
    10201 ttaaatggac ggttttaaca gtattaccag ctgcatctgt tttagtagcc acgttaggaa
    10261 aagcgtatgg atggaatgga acagatatga cagtactcac tatcaatgca gtagcaacat
    10321 ttttaggcgt tatcactggt gtgtctgcat ataacttaaa aaaataggag gaaaaaaatg
    10381 aagaagaaaa tcattttatc attgagccta ataatggctc tttttttatt gccaattaac
    10441 gggttcgcct atacgatcaa caatgaattt aatttaggtg taaatgaagg tagctcacaa
    10501 gtagcaaata accaatacat cctattgcat gaaacagcaa acgaaactgc gacaggacga
    10561 aatgaagcgc agtacatgaa acgttcttgg tacaatgcct atacagcgta tattgttggc
    10621 gatggtggga tcgtttacca agttggacaa cctggttatg tacaatatgg cgctggttca
    10681 tatgctaatg caaatagtcc tgtgcaaatc gaactgcaac atacgcacga taaagtaacg
    10741 tttgaaaaga actacaaggc atatgttgaa ttagcgagag attccgctaa taaatacggt
    10801 attccactta cattagacac accgtataat caaccaggaa tcaaatcaca tttatgggta
    10861 actcaaaaca tttggggcga tcacactgat ccgtatggat acttatcaga aatgggtgta
    10921 agtaaggaaa aactggctta cgatttagct catggattta cagacgaaaa cccaacgact
    10981 tctgagaaca agcctgtcat tgatccaacc cgagcaggtg cagcaaatcc aactttatca
    11041 gatggaacga accattctca cattgatcag tttggagaaa tcgaaaatgc gaacttacac
    11101 gtcgctggat ggcacatcgc taactataaa tacgagtata ttttcattat ggactacaat
    11161 actgggaaag aattagctcg agtaagagct gatggaattt atagaccgga tgtaaatcaa
    11221 gcttataata cttctggaaa cgttggctat catgtatctt ttaacatgcg caatttccct
    11281 aataagaaag tatacgtcat gatgcgggca acgaatgatc cagaaggaaa cactaaaggc
    11341 ggtgcgcaag atttccatga caaacgttgg tatttaaata ttcctaaacg ataaaaatag
    11401 ctcctcgttg aggagcagta catagaattg aaaattaacg ttaaatcaaa aaaatattta
    11461 caagatgttt atttaagtag ataataaaag aaaaaagcag gtgaaattgt gacagcagaa
    11521 attggtatta tgaataaaag tggaatcgtg ttagcttcag attcagcatc tacgattgga
    11581 gatagcaaag tatataatac tgcaaaaaaa ttatttactt tggattccat gcattctgta
    11641 ggtatcatga tatatggtaa tgctgaattt aatggtattc cttgggaaat aatcattact
    11701 caatataaga aaagcattgg tagttctgtt tttaatactt tggaagaata cgcggataat
    11761 tttatagaat ttgtcaaaac agcatctttt attagaagtg aacagaccga gcaagaacaa
    11821 atgattgggg tttttcaaaa aataatttca ggtttatttg agagtataga agtagatatt
    11881 aactttttaa taagccaagg tacacaaata gacaaagatg tcctagtgaa gttattacaa
    11941 acaaaaatga ataccaattt gtctcaacag tcccagacat tcattttaga tattgaaaag
    12001 gcgttatttc ttagtaacta tggagaaata ttaaaagata ttcttaatag tatttctaca
    12061 atggaaggtg tttcagaagc tatatcagag gaaattcaaa gttacgttta tgaaattata
    12121 attagagacg atgtttattc ttcccctaca ggaatagtta tagcaggata tggaagaatg
    12181 gatattttcc ctaaactata ttcttataat atgtttggat ttgttatgaa tattctgaaa
    12241 tactcagaat atgaaagtgc tcaaatagga aatgacaatg gttcattaag atctactata
    12301 ttaccatttg ctcagtctga tgtggtaaat actgtagttc aaggggtaga tccacaaatt
    12361 actaattatc tgtcgagtca agtcgatagt tttgacgata acggaaaaaa tacctatatt
    12421 aatattataa agaatatttc agagtttcaa caaaatcaat tcatatttcc gttactaaac
    12481 atgattgctt tactcccagt tgaagaaaca gcaattatcg cagagacttt attaaattta
    12541 accagtttta aacgaaaata tactacttct gtagaaacag taggtggccc aattgatgtt
    12601 ttag
//
LOCUS       DASJON010000076        12604 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJON010000076 DASJON010000000
VERSION     DASJON010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.076"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..542
                     /locus_tag="U3989_001540"
     CDS             99..542
                     /locus_tag="U3989_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1714993.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="U3989_001541"
     CDS             complement(548..1732)
                     /locus_tag="U3989_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN1714994.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="U3989_001542"
     CDS             2000..2698
                     /locus_tag="U3989_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1714995.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="U3989_001543"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="U3989_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN1714996.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="U3989_001544"
     CDS             complement(3508..4041)
                     /locus_tag="U3989_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1714997.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="U3989_001545"
     CDS             complement(4338..6719)
                     /locus_tag="U3989_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1714998.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="U3989_001546"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="U3989_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN1714999.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="U3989_001547"
     CDS             7420..7959
                     /locus_tag="U3989_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN1715000.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="U3989_001548"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="U3989_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN1715001.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="U3989_001549"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U3989_001549"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="U3989_001550"
     CDS             9066..10232
                     /locus_tag="U3989_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN1715002.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="U3989_001551"
     CDS             10278..11774
                     /locus_tag="U3989_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN1715003.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="U3989_001552"
     CDS             11855..12538
                     /locus_tag="U3989_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1715004.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4234 a         2204 c         2598 g         3568 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttct
//
LOCUS       DASJON010000077        12472 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJON010000077 DASJON010000000
VERSION     DASJON010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.077"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..267
                     /locus_tag="U3989_001553"
     CDS             <1..267
                     /locus_tag="U3989_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715005.1"
                     /translation="YSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEAEGFIYPF
                     CGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            754..1167
                     /locus_tag="U3989_001554"
     CDS             754..1167
                     /locus_tag="U3989_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN1715006.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1787..1951
                     /locus_tag="U3989_001555"
     CDS             1787..1951
                     /locus_tag="U3989_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715007.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            2363..2908
                     /locus_tag="U3989_001556"
     CDS             2363..2908
                     /locus_tag="U3989_001556"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715008.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            2909..3226
                     /locus_tag="U3989_001557"
     CDS             2909..3226
                     /locus_tag="U3989_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1715009.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(3383..3547)
                     /locus_tag="U3989_001558"
     CDS             complement(3383..3547)
                     /locus_tag="U3989_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEN1715010.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            3649..4170
                     /locus_tag="U3989_001559"
     CDS             3649..4170
                     /locus_tag="U3989_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN1715011.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            4157..4630
                     /locus_tag="U3989_001560"
     CDS             4157..4630
                     /locus_tag="U3989_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN1715012.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(4637..4795)
                     /locus_tag="U3989_001561"
     CDS             complement(4637..4795)
                     /locus_tag="U3989_001561"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEN1715013.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            4888..5649
                     /locus_tag="U3989_001562"
     CDS             4888..5649
                     /locus_tag="U3989_001562"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1715014.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            5869..7188
                     /locus_tag="U3989_001563"
     CDS             5869..7188
                     /locus_tag="U3989_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1715015.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            7204..8424
                     /locus_tag="U3989_001564"
     CDS             7204..8424
                     /locus_tag="U3989_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1715016.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            8459..9136
                     /locus_tag="U3989_001565"
     CDS             8459..9136
                     /locus_tag="U3989_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1715017.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            9133..10326
                     /locus_tag="U3989_001566"
     CDS             9133..10326
                     /locus_tag="U3989_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1715018.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            10341..10667
                     /locus_tag="U3989_001567"
     CDS             10341..10667
                     /locus_tag="U3989_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1715019.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            10671..11006
                     /locus_tag="U3989_001568"
     CDS             10671..11006
                     /locus_tag="U3989_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1715020.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            11003..11398
                     /locus_tag="U3989_001569"
     CDS             11003..11398
                     /locus_tag="U3989_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715021.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            11395..11766
                     /locus_tag="U3989_001570"
     CDS             11395..11766
                     /locus_tag="U3989_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715022.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            11782..12342
                     /locus_tag="U3989_001571"
     CDS             11782..12342
                     /locus_tag="U3989_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1715023.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
BASE COUNT         4276 a         2161 c         2769 g         3266 t
ORIGIN      
        1 tactccagca gtcatggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
       61 acacgtgaga aaccaaaatt caaagtcggt gacatggttc gctgtgaagc agaaggattc
      121 atctatccat tttgtggata tgtagaacat ctctataatc actcagcaat cattcgtatt
      181 gaaaacacga tggaatgtga caagtggtta gcgaaaagca aagagaattt agcagtagcg
      241 agattggtgg atatggaggt tatataaaca gcgaaaaaat gtacttctaa gagaaaaatt
      301 tattttttat aaaaatagga aagtatcatt atgaaaaaga ccacagagat aaataaacct
      361 accacataaa aaatgaatat ttcttgcgat tcaatatagg tatatttaac aatttgtaca
      421 ataaaaaata ctataggtaa aaaagataac atgaggagct tatttgttct agagtgtttg
      481 acaaagtaac aaaatgcgac gataccaata caaacgggaa tccaaaataa cagcatgctc
      541 cacatagtca ctcaaccatc tcctatacgc tttattatca atagataata tcatttttcg
      601 taataaagat aaaaaaataa gtaaggcttc tcattaaaat ataaaaaaga cagccgacca
      661 ctgactgcct atataagagt attgaaataa aaagctgctg atataataaa ttccacaagt
      721 ttattatatc acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc
      781 aaacagacaa gatgtaatgc gagagatgtg ctgaagaact ttcggcgttt ggagcggatg
      841 gcaggtcgct ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcgccg
      901 aagcacggca ataaggcaga agacgcaatc attcagatga tggatataga agcggagaga
      961 gacgcgattc tagcagcctt gatggctctt agtctgatta gtcgtcagat actctactat
     1021 agcttttgtg tgccagatag cttctcaaac tacagaatta gccgtgaagt gggttattca
     1081 gaaagaagta tacaacggat gaagtcggaa gctctaatag agtttgcaga agcatataaa
     1141 cacggaagaa taattgctta taaataattt ggcggctttt tggcggaatg atggcggttt
     1201 ttagctattt accagtgata ttatggtagt gtcgaaagat tagtgatagg tctaagacaa
     1261 aataataata aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc
     1321 aacggaaata ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttattatc
     1381 tatcatccgt tgctgtctat tgttttttta attattcaca cgataactaa aggtgagcga
     1441 agagaaatga ttccattaat aatttcaatt tttgcgcttt gtcttaatgt ctatatgatt
     1501 ggatttaaaa atgggcaaaa taaaagatag tagtaactaa gaaagaaatt tttatattgt
     1561 cactgtggcg gaaagggtag acgctaagca tgtgtgctag gtcaatgctt cggcaactat
     1621 gcaatgttcg attcattgcc agtgactttg gcaaccgagg catcggcggt ttaaaaatat
     1681 aggggtgcgc aatttcgtac gcgttttgtg catcgtgtag gttgctatta catattagat
     1741 cactcgttga gtggtctttt tattttacat aaaggaggta acaacgatgt atagaccaca
     1801 atacttagaa cagaagtatg aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg
     1861 aaagtataga agaccaataa agagcgatac atataaacga aaggaaaaca atgaagttat
     1921 ttcttttcgc agaaggagaa aagccaaatg agaaactact ggtatgtatc gctaactaat
     1981 gaatatcctc gaaccattga tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa
     2041 gggaagtact ctattgtcga aatgctaaga gaagctacac caaacgaagt ggataaatgc
     2101 aagctgatat attgcggtca tggctattgg aaagacgagt atatccaaca aaacattgag
     2161 aggtggatag atagatgagt tacctcgaac atttgaaacg ttgctacatg cattctaaga
     2221 ataaacttcc tgacagctac actgtagatg atgttgctat acatgtattg aagactgaga
     2281 gccatagcag tcctgatggt agcagtaagg agcagacgct cgcgtggttc aagttcttta
     2341 agtggattaa ggaggaagag taatgcacat gcacatagga gaaaagatat ataaattgag
     2401 acggcttaat aacgaaacaa tgaacgactt agttaaacaa actggagtgt caaaatcatg
     2461 gttatctgat attgagtctg gaaaaaagca acgcgttgat tttaataaga tatgtgcaat
     2521 agccaaacat tataaagtta gcttagaata ctttcgggat gattatttgg atagtgatga
     2581 acacgaggat attattcctt tgacaggcga aacaagagtt aaatcagtga aatcgcttga
     2641 acaaagccta aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa
     2701 agatactgac tcagtaccag aggtttggta caagggtgag aggttggatg aattgcctaa
     2761 aggattagta gatgtctcgt atcattggaa gactgatgat tttactaatg atgatagagg
     2821 agcgaacgac atcacgattc aatacttttc tagctttaat gataagtatc cagataagaa
     2881 aacaatcgga cacaagagag atatgtaaat gaaagaagtt agacctagag acgaaataga
     2941 caaactatac aagaccaaac gatggcgaga cctaaggcaa gtagtaatag ctagggactt
     3001 cggcatgtgc caagagtgca agcgtcgagg gcggaacaca aggggcacga tcatccatca
     3061 catagtcgag gcgagggaag acctgtcact gttctggtcc gtagataacc ttgaatgtat
     3121 ctgtgtagct tgtcacaaca gagagcatcc agagagatca ggcgggaaga agaaaccaaa
     3181 acctaaatca catatcgtta aaatgtattc aactcctgaa agataagttt gcagcgaaat
     3241 gaaggtagcc cccctactct aaaagattaa agagtaaggc ttgagaagaa cggtgctgtc
     3301 cttccttcgt aaaaagaccg cttttcaagt tttttggaga aaaaggaaaa agccgaccaa
     3361 tttaagccgg ctttggacga agctatttct tagtccattt gtttcctttt tgagaagtag
     3421 ggggtaatcg gtcgcctgga tcaattttta cttcttgtcc gccttggaca tttccaccac
     3481 gaggacccac ttctttgtag gttccttttg gtttgttgtc ttcgccgggt ttatagagtt
     3541 ctcccatagg aatccctcct taacaaattt cggcacagca ctgccgataa cctaattata
     3601 aggattgtga taacgatttc aatctatctt ttgaaagaag gtgatattat gccgcaacca
     3661 gcgaagagtg caaaattaca attattaaac ggaaacccaa ataagaagaa taccgaagaa
     3721 ctccgcaagc gagcggccgc agaagacaaa ttaaaaatgg ctactgacaa aatcaaaccg
     3781 ccgttatggc tagattcgct aggaaaggat acctttgagt ttatcgccga tgaattgctg
     3841 tctgtggatt taatcagtaa tccggacgtc catacaatgg ctctatactc caattggtat
     3901 tcgcaatacg tttctttaga aaaacagctt cgaaaactac aacgagagta caagttgaac
     3961 tatgcgcttg cgaaaaagga ggcagaggcg agaggtgagc cgtttaatga acctaatgaa
     4021 ttaattggta atccgctctc tcggcagatg gatacagcgt ctcggaatct ccgttctttt
     4081 ggcgctgatt taggactatc accagcagcc agagctaagt tagctattaa gatggctgat
     4141 gatggtggtg atgacgatga cgacttctaa tattttggat atgtcttaca cagaacgtgt
     4201 ggactattgg caaagctatc ttgaggagca agcttcttgg ggtggctttt taaaatgtcc
     4261 atatccggaa ttgttaacta cttggtatgc ggaacgatta atcgatggaa gcataccagc
     4321 cagcaaagaa aatattcaag ctgctaaacg gcatatgcgt gatttgcagc gccaaggaac
     4381 agatgatttt ccttggatct ttgacgaaga aaaaggtcac cggcctatta gatatatcga
     4441 aaaaaaatgt aaaccaactg aaggcgactt tggttcgttt gttttgcaac cttggcagca
     4501 tttcataatt ggatccatgt acggatgggt acatcgtgat acaggagagc gtcgcttccg
     4561 cgaggctctt atttttgttg gacgtaaaaa cggtaagttt tgccgtttaa aaatcgggca
     4621 aaatcggtga aaacctttat ttttggcttt cttttagtaa taattcgata gctttatcaa
     4681 gcaatttaga cataggaacc atagtttctt ccgacatttt ttttaatcct tcgtataaat
     4741 ctttatcaat tgcgttagaa attctaattc tgttttttag tccgtatttg tccatgtgtg
     4801 acactccttt ttattttact ataacatccg attccaccgc ttgcaactac catcaattga
     4861 tggtagtata taaaagaggt gagtagattg aaaaaataca ttatttacaa acacttaaat
     4921 aaaataaatg gcaaaattta tatcggtgtc acaaatgata ttggcagaag gtggaggagc
     4981 ggtgggattg aatataaacc accaaagaac gaaacccaac accacagaag tttttggaat
     5041 gcaatacaaa aatacgggtg ggaaaatttt gatcatttga ttattgaaga agacttaacg
     5101 atgaaagaag cttttgagaa agaaaagttt tatattgaac tttatgactc aactaataag
     5161 aaaaaaggtt ataacatcgc aaaaggcgga aatggcggaa taatatataa agttcacccc
     5221 aaaggaatgc tagggaaaaa acaatctaaa gaattttctt caaatcacag taaatgggca
     5281 aagaaccata agaataattg tatgacaaac ggtgatgttg tatggggtgt tactcacgaa
     5341 cacccaaaag gcatgttggg aaagcaccac agcaaagaat caattatgaa aaagaaagca
     5401 tatagtggtg aaaatgctgt aacctcaaaa ccaattgtcg caattgaacg ggatggaact
     5461 aaaagagaat ttcacagcgc taagctttgc atggcttact attcgattag tacatgtgtt
     5521 ttttaccgac ttttaaaaga tggcgcccct tatgtgataa atcctaaagc gaactacaga
     5581 aataaagaaa aaatattagc tatcgaagga attatgttca aacataaaga agataccgag
     5641 gtaagttagt agattgcgaa aggttactaa ctaccgtaac gcgtaggagt tgaataaata
     5701 taatgctccc aagagtgtcc gacagcagat aatttgctga aaatgtacgc taaactgggc
     5761 cagaactgac tgaccgatga aaatgagggt gacctccaga gtgcgagata aaaagctcgc
     5821 agataataac aatcgaaaac atcactaatt tcaggccttt ccacatacat ggtcgcttat
     5881 gatgatgaac aaggcgccaa cgtttacgta ttggcaaatg ctcgtgatca agcaagcttg
     5941 ttgtttgata aggccgcaga aatggtcaaa caatcgccgg cgctctttaa gaaatttggt
     6001 aagcctaaac gatcaagtat taattatgct cccgcctttt ctaaaatgga accacgcgcc
     6061 tcagatagcc ggaaattgga tgggctaaac actcactttg gtatttttga cgagatccac
     6121 gagtttacga attacaatct gatcaacgtt atcaagaaat caagaggaac cagaaaacag
     6181 cctctgatag tttatatcac aactgctgga tatgtattag atggtccgtt gatgtcttat
     6241 tttgagcaag gtgtggactg tttggaacat ttggaagatg acatcgatga acggactttc
     6301 tattatctgg caaaacttga cagtgcggaa gaggctgatg acccaagatt atggatcaaa
     6361 gccaatccga atatttgtct aatgaatttt gttggcatgc tagatgacta tgttaaggat
     6421 aaaaaagatc caaaagaata tgctgactgg attaccaagc aatttaactt gttttccgat
     6481 atcgatgagc tgtcatttgt cgatatgcct accattaaac gaaacaataa aaccatcgat
     6541 attgaaacgc tcaaaggtaa gaagtgtgtc ggtggttttg acttgtccga aacggaagac
     6601 tttaccgcag ccgttttaga atttccgctt gaaacaggcg aggtattcat tttgcaacac
     6661 acatggatcc cacaagctag atttgatcga gataacaatc aagagcgtat caaagcgtgg
     6721 gagaaggtgg gagatctaac gattattcct ggtgattacg tcaattatga atacgtctta
     6781 aattggtttg tagaaaattc gaaaatctat gacattgtaa aaatcaatta tgacaaggcc
     6841 aaggcgctac gattaaataa agaactagaa aatgcaggat ttgaaaccaa cgagattcgg
     6901 caagggtttc tatcattagg tgggccaatg caaaacttca aggaaatgct attggacggt
     6961 aaggtgattt tcaacaattc caagctttac cgatggtatc tatccaacgt caagctggtg
     7021 atggatcgca actcaaactg gatgccgtct aagcagtcca agagtagaaa aatagatggt
     7081 tttgcagcaa gtttgaacag ccacgccgaa gtgttgaata tgttggttaa tcctgtcgga
     7141 accgggaaag taacctatta ctcgatttcc gatttaatga atatgtaaga aaggtgtgga
     7201 ggaatgagta ttttagatcg tttgcgttct tttggccgag cgaagccgaa agcgagcaaa
     7261 caagagtatt ttttgaatga cccgggattg ataccgtatt tagtcggaaa agatgaaata
     7321 tcagaaggga ttttttccgt aattagccgt gtatcgaacg tttttgcgtc tctccctctc
     7381 aaaatgatag atgtggagtt tggccaaccg gacgactgtc ctgcatacaa cttgttgagc
     7441 gaaggccctc gatattttac aaagtttgat tttttccggg acgtggaagt tttgagaaac
     7501 taccaaggga atgcgtatgt gcagattttc cgaaatatca atggagaagt agcagatatg
     7561 gcgttagtaa aacctggtgc ttgccatcca gtgattgata tggatagcgg ggagctttac
     7621 taccaagtaa ctgcgactga caaaggcagt tacaagcaag ttatctatgt acattacatg
     7681 gaaatgcttc actttaaaca accgaggttt ggcggcttgg aaggtacaga ccccacaaaa
     7741 gtattaacga ataccctcgg atatgatcga gaagtccgaa aaatctcttt aagtcagctt
     7801 aaaggaagta atgaagggct aaaagttaag tttgctagca atatggatga agaagctaaa
     7861 aaagctacag ttaaaaacat tgctgatttt tatcgacaaa acggtggact acttgtggaa
     7921 gaaaacggtg tagaaatcga acgtttacaa cgagagctgg tagacagcaa gcttttagat
     7981 actgataaag tatctcgctc cagaatcgct atggtctaca atgtgccgga gcatttcatc
     8041 gggaataacc agtcgagtta ctcctctcag gaacagctca atatggaatt tttgacgtac
     8101 aatctagtac cgaccgttaa tcaatatgaa gcggaactaa ataagaaaac actatcgaga
     8161 accgaaaaag caaaaggtta tcgatataag tttaatgtcg caagtttgct aagagctgac
     8221 actcaggcaa ggggacaatt ctatcaaatc atgcgacgag gcggagcata ctcagcgaat
     8281 gatgtccgaa gatttgagga cttgcaaccg ataaataaaa ccggtatgga tgattaccat
     8341 atttccggag acctatatcc aatcgatatg gatccaacat taagaaaaac aacctcgtct
     8401 aaaagcgtag ccgaaaacgg ttaggttttt ttagtttgca ccgaagggag gtggaaaaat
     8461 gaaaaaagtg acgttaagcg gcgatgtcgt ggataacgat accgcgtggc tttatgactg
     8521 gtttgggatc aattgtatct caccagggaa aatttctgcc gctcttacag aagcagcggg
     8581 ggatgaagta gaacttgata tctcatcgaa cggtggggat gtcctagcgg caagcgaaat
     8641 atataccgct atccgtgcct atccgggaaa ggtatccgga aatgttgtga gtattgcagc
     8701 aagcgctgca agtgtaatcg cttgcgcttg cgaaccgctt agaatctcac ctacggcaca
     8761 catcatgatt cataacgcat gggtgaccac tagtggcaac gctgaggaat taaaagccaa
     8821 tgcagaaatg ttaagcagtg tggatgagtc tattgttaat gcttacgaga tcaaaacagg
     8881 actcgatcgg aaaaaacttg ctgatttaat ggcgaaagat acttggttaa atgctcaaac
     8941 agcagtagca gaagggtttg cagatgaaat tatgtttgca gaagcaccag taacggtact
     9001 caatgcctct caaccggtta ttccaaaaaa cgcagtaact aagttgaaaa atttaatact
     9061 caaagcggaa gcaccgcaag aagaaacact cttacagaaa aaactaaaag ccttaaatgg
     9121 agggaaaaac gaatgaattt agaacaatta aaaaatgcgt gggtcgaggc gggaagtaaa
     9181 gtctctgact taaatgcaca actcaatgca gcattggttg acgatgaaaa aacagaagaa
     9241 gatgtagtaa gtttgcaagc acaagtaaaa gcagcacggg ctaaacggga cggattgaaa
     9301 gagcaagtgg caaatatgga agccgaacaa gtcttaaacg tcaaaaaaga accattagat
     9361 aaaaaagatg aaaacttgaa aaacaagttt atcaaagact ttaaagcgat ggtcaatggt
     9421 gatcctgcta ttatggctac tttgacatct aatacggatg aatctggtaa tgctatcgga
     9481 ttgactattc ctgtagatgt gcaaacgact attcatactt tggttcgtcg gtttgactct
     9541 ttgcaagaat acgtaaacgt tgaaaaagtg accactacca gcggttctcg cgtttatgaa
     9601 aaatggtctg atattacacc gttgactgct ttggatactg aagacggtga aatcccagca
     9661 aatgatgatc ctgcacttta cttgatcaaa tacttgatca aacgctatgc aggtatttcc
     9721 acagtaacta acagcttgct aaaagatact gccgaaaaca ttttggcatg gttgtctaaa
     9781 tggatcgcga aaaaagtagt tgttactcgc aatacaaaaa tcttggcagc tattgatgga
     9841 atcaaagcgg cacaaaagaa agatgttaca gatgttgatg gaattaaaga tatcgtaaac
     9901 gtccaacttg atccagctat cgaagctaca tctatgttta ttacaaacca agatggcttc
     9961 aatgttttag ataaagtgaa acgtgctgat ggatcttact tgttacaaaa agacgtagct
    10021 tctgcaactg gatatacctt cttaggtaaa ccaatcaaga aaattgcttc tcgtttcttg
    10081 ccaaacaaag gtacgcaagc tactcctaaa tatccactgt acattggtga tctgaaagaa
    10141 gccgttacat tgtatgatcg cgaaaacatg agcttgctga caacgaatat tggtggtgga
    10201 gcttttgaaa cagacaccac taaagtacgc gtcatcgatc gcttcgatgt gcaactagtt
    10261 gatgatgaag cggttgtttt ggctactttt acaactattg cgaacgagac accggcgaaa
    10321 gtttaaggag ctgattcctt atgattcttg atcctaaaat ggatttaggc gaaatcaaaa
    10381 acgcactaaa gattgatacc gatgatgacg atgtggaagt aagccgtgcg gcacaagctg
    10441 caattgcata cattagaggg gctatcggga atgataagcc ctctttttat aagcaagaaa
    10501 atgaaacggt tgatctgatt aatttagcta tcctgcaatt agcagatcat tattacaacg
    10561 ctcgatcagc aaccgtgagt gggaacttgc gagagtacga tttaggtttt acaagcctaa
    10621 tcttgcaact caaagcaagt tatttgcttt ttgtggagga ggagtagcgt atgcccctta
    10681 tccaaacagg aaatttaaat caacgcatca agtttgtccg agatacgacg gttaaggatg
    10741 aggacgggca agttgtcccg acttctacaa ccattcttac ttgctgggca agtgtgcaga
    10801 cacaacgcct gaacgatatt aagacgtcga ttggtacggc tttggaagga acactgacgt
    10861 tcattatccg ctaccaacaa aaatcagagc tgaccaatga tatgaaagtg cgttggaatg
    10921 gaaaaacgtt tgaaatcatt acgattacga aaggcgagtt tgcgaaggac ttcacgacaa
    10981 tcattgcaaa agaggtttca aaatgagtgt agaagtcgat gcaaccgaag tgtacaaagc
    11041 gcttagggaa gtaaaagcaa acgttcaacg agtggaaagc ccagcactta gaaaagctgg
    11101 ggagtacgct caagaagagt tacgacaaaa cacaccttac tgggatggaa cgaagtcaaa
    11161 cggtaaacgt ggttcgtata tgcaagaaca tgctaagaac catgtggtta caagctcgat
    11221 aaaaaacgga ttggtagaag tcggctatga caaagatgtt tcttggcgga tgcactttat
    11281 cgagttcgga acaatcaaac aacgtccaaa aggtttcgta caaaaaacac aaaagcaaat
    11341 cgaaaaacaa gtaacacaaa tcattgctga cgaagtaaaa aggaggctag gactttgaaa
    11401 acggcagtat cacaagtcta ttcaattctg aatagcaatg aaaaaacaaa gaacattgat
    11461 ttttacacca atagtgttcc ggaatcagct caaacagtac ctagccttcc agttggcaga
    11521 attacagaga tatccggcaa ctatgaagat tttgcaagca acaatccttt gaccattcaa
    11581 tttaacgtac aggtagatgt atgggtgtca accatgaaag aggttgatgc cttttatttt
    11641 gcccttgatg aggttatgag ggggaatggt tggcaatgcg catacacgga acaaacagat
    11701 gacgaggact tggaaggtgc aaagcggatt atcaaacgat atgtagcaaa tatttcacta
    11761 aattaaaagg agagaaaata gatggcaaca gtaggattcg agagcgtcat ttttggcgta
    11821 aaaacaggtg caggcggcac tctaaaagaa ttagtagcag ataagtcgaa aggcggagcg
    11881 atcgaagcta aaattactgg attaggcgca acttctaaca caacatacgc ttcaaacgta
    11941 ccgttcttca ttgcaagtaa aggggtttcg tcgccaaaag ttacgcttga cgtggcagac
    12001 ttaatggata acggcattta cagcgaaatc attggtgcta aaaccgtgga tggtgcaaat
    12061 gtaattggtt cagaaactga agcgccttac gtgtcggtag tcatggttac agcgaacaaa
    12121 gaaggaaaac gcttgttcat gggattgaca aaaggaaaat tcagtcatcc agatatcgac
    12181 atgaaaacag ctgaagacaa aggggtagaa ttgcaaaccg attccatcga aggggaattc
    12241 atttctgatg aacgtggcta tgtatactta acagccgtag aatcagaagg aatgacctta
    12301 caaaaattca aggacttggt aaataacaaa gagggggagt agttaaccct gcatctacac
    12361 caacgacaga tacagggaca ccaaaagaac cagaaccaaa aattgataca caaggttagc
    12421 catttttggc tagccttatt ttttgtaaaa acaaggagga aaacaaatga tt
//
LOCUS       DASJON010000078        12311 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.078, whole genome shotgun sequence.
ACCESSION   DASJON010000078 DASJON010000000
VERSION     DASJON010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.078"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..477)
                     /locus_tag="U3989_001572"
     CDS             complement(<1..477)
                     /locus_tag="U3989_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN1715024.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINA"
     gene            complement(607..1317)
                     /gene="budA"
                     /locus_tag="U3989_001573"
     CDS             complement(607..1317)
                     /gene="budA"
                     /locus_tag="U3989_001573"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN1715025.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1330..2994)
                     /gene="alsS"
                     /locus_tag="U3989_001574"
     CDS             complement(1330..2994)
                     /gene="alsS"
                     /locus_tag="U3989_001574"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN1715026.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3199..3861)
                     /locus_tag="U3989_001575"
     CDS             complement(3199..3861)
                     /locus_tag="U3989_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1715027.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3871..4686)
                     /locus_tag="U3989_001576"
     CDS             complement(3871..4686)
                     /locus_tag="U3989_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1715028.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4948..5391)
                     /locus_tag="U3989_001577"
     CDS             complement(4948..5391)
                     /locus_tag="U3989_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN1715029.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5525..5863)
                     /locus_tag="U3989_001578"
     CDS             complement(5525..5863)
                     /locus_tag="U3989_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1715030.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5851..6228)
                     /locus_tag="U3989_001579"
     CDS             complement(5851..6228)
                     /locus_tag="U3989_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1715031.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6452..7645
                     /locus_tag="U3989_001580"
     CDS             6452..7645
                     /locus_tag="U3989_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1715032.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7811..8233)
                     /locus_tag="U3989_001581"
     CDS             complement(7811..8233)
                     /locus_tag="U3989_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715033.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8628..8822
                     /locus_tag="U3989_001582"
     CDS             8628..8822
                     /locus_tag="U3989_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715034.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8819..9313
                     /locus_tag="U3989_001583"
     CDS             8819..9313
                     /locus_tag="U3989_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715035.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9475..10647)
                     /locus_tag="U3989_001584"
     CDS             complement(9475..10647)
                     /locus_tag="U3989_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1715036.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10853..11668)
                     /locus_tag="U3989_001585"
     CDS             complement(10853..11668)
                     /locus_tag="U3989_001585"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1715037.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11818..12264)
                     /locus_tag="U3989_001586"
     CDS             complement(11818..12264)
                     /locus_tag="U3989_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1715038.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
BASE COUNT         3607 a         2481 c         2137 g         4086 t
ORIGIN      
        1 agcatttata ttaaaatttg ctaataaaag atctacatat ttttctagaa atgcttgatc
       61 gatttcatca taccctttcg ttactccgca atcgatatct aaaacaccga ttaaattacc
      121 ttcttttacc attggcacta cgatttctga ttgtgcggct gaatcgcagg aaatgtaatt
      181 tgcatgggtt ttgacatcat caacgatcaa tgtttcttgt ttttcagctg attctccaca
      241 aactcctttt cccatcttga tacgtgtgca agaaactcgc ccttggaacg gtcctaaaat
      301 caattcatta ccatcgaata aataatatcc tgcaaagaca gtctcaggca aagtctcatt
      361 caacaatgca gatgaatttg ctaaatttgc taaccagtta tcttctgctt ctaataatcc
      421 ttgttgctgt aataataaaa tttcgtatgc atcttttttc tgtttttctg tccacatgat
      481 aaaaaccgct cctttttgat ctatggagaa gattatagct cagttttttt gtaaacgcta
      541 ggtttcctcc tcaaaaaaac gagatacgtc aggttaatca ccttgaacgc atctcgctgt
      601 ctttttctat tctgcttgtt ggatttctgc tgctaaatca gcataatcaa tcgtttcgtg
      661 aacaaaagtc ggatcatcaa ctggaaaatg ctgtacaagg gacgagatgc ggctaatctc
      721 aacttttcct tcagccactt caaaatccat tacatgtccg ccaaatgtat gtgtatcatc
      781 gataaaatgc aagtgaaatc ccgctgcagc aactccttgg aataattcag gcgtaaagaa
      841 cccgactaat gttccttgga cattgctttg ttggaattct ggttgagact ctgaaatttt
      901 tgctagtctt ctgtaaggtt tttcttgctt cggcatgata cggatgtgca tatttttgaa
      961 taatccagtt atcttcactg ccaaaaatag gttttccccg gcttctctta ctaatatgtc
     1021 ttttttcaat tgttctccat ctgcagtttt tgagacagaa aaactagtat ctggcgtaaa
     1081 atctgtaatt gctgcatagg gggtaagttc acttccatct aattgggcaa ctgagccatc
     1141 tgatcttcct tgataagcta cgccatctaa aaagatgact tcaccatcaa gcccatgcaa
     1201 tgttcccaat cccagagttc ctttttctaa tatatgagca atcgtttctg ttccatccat
     1261 caagccagcc atcaaagctc ctaatgtccc gtgctggtat aagatttttt cagtcatgtc
     1321 gacacttcct taatagaatt gatctggtaa gattgtttct cccaatttct cattatcacg
     1381 gtaatcaatc ggaatatcaa taattacggg tccttctgtc gcaaatcctt cttctaaagc
     1441 tttttctaat tcctctacac ttgtagctcg gattccttta gcgccaaaag cttctgcata
     1501 tttcacaaaa tcaactggac caaagtctac acctgacgcc cgctgatatt tcattttttc
     1561 ttggaactct accatattgt agtgtccatc attccaaatc aaatgaacga tattcaattt
     1621 tttacgtacc gccgtttcta aatcctgggc agagaataaa aatcccccat cgccggaaac
     1681 tgatacgatt tgtgtatttg gacgcaccaa tgcagcagaa atcgcccatg gcaatgcaac
     1741 accaagcgtt tgcataccat tactgaataa caaatgacga ggttcataac ttctaaagtg
     1801 gcgtgccatc caaatataat gactacctac atctacggta acagtcatat catctgttac
     1861 ttttgactga agcgtgttaa taacttctaa aggatgcaag atacctgctt ctccttttga
     1921 ttgaacaatg tcccgctccg ttaattctgc ttgaagagat gctaaatatt cttttgcatc
     1981 ttctgacact tgttgaggct ctaatgaacc tgtcaataaa tcaagagtgg ctgagatatc
     2041 accgatcaat tctctttcag gctgcataaa aggatcgatt tcagctggcg cttcatcgat
     2101 cacaatgatc cgcgcatctt tttccgcatt ccagtttctt gcttcatatt cgattggatc
     2161 atagccaata gcaataacta gatcacttcg tttcaaaagc atatctcctg gttggtttct
     2221 aaataaaccc acacgaccaa agaaatgcgc ttctaattca cgtgagatca ctccagctgc
     2281 ttggaatgtc tctacaacag gcaatgctgt ctttccaact aattgcctga tggctaacgt
     2341 ttctttttca cttgatcctc gcatacctac taatagtacc ggtagttttg cttctctaat
     2401 gcgttcagct aggtaacgga tatcatctgc agaagcagaa cctaatttag gatcgctcaa
     2461 tggcttgatg acattccctt ggacaggtgc atccactaca tcttgcggga tactgataaa
     2521 gctcgcgcct ttttttgaac tcttagccat acgataagca ttggcaatca cttctgaaat
     2581 tgtttctgga tcttggattt ctgcactgta ttttgtgatt ggctggaaca gtgctgcatt
     2641 atccatgctt tgatgggtca acttcaatag atcgcttcgt tttacttgtc cggcaattgc
     2701 taaaactggg tcaccttctg ctgtcgctgt taccaaacct gtagctaagt tactagcacc
     2761 aggaccactt gttgcgataa ctactcctgg ctctcctgta atacgtccga ttgcttgtgc
     2821 cataaatgca gcattttgtt cgtgacgagt cacgattaat tcaggacctt gatcctctaa
     2881 ttcattaaat acaccatcga tttttgcacc tggtataccg aatacataat caacatgatg
     2941 attaatcaaa ctttctacta cagatttcga accttgtttc attttttcgt ccattgaaca
     3001 tcgtcctttt tatgctttat ttttaattat tagttagaaa taatcacaaa taattgatac
     3061 agtttctaaa cctgtttcat tcaaaagtat agcacgtttt atttcatttg tttagccata
     3121 tgtttcaaaa aatgaaaaag aatgacagtt tttacataaa aactgtcatt cttaattgaa
     3181 agtttcaatt tataaagtct acagatccat agttctcatc aatttacgat tgttgaagta
     3241 aatcaaacca aatacgatag aaggacctag tagaatacac cattggggct ttacgaaaaa
     3301 gaagagaaac aaacttgtca atagtaaaaa atctagtaaa ctcacatacc attttttcaa
     3361 cgtatagtag aacgctatcc gtagaacttg atttggtttc gacgctggat ttctcacctg
     3421 aaaataccag tagttgatgc taaaggaaac tgataaacaa gcaagtaaaa caaacaacgg
     3481 aatcaaccat tttccataag aaaaacgtat aaaaaagaaa caatcaaaaa tcattatcac
     3541 actaactatc caagcaaata gccaataaaa aaatcccttt agccaaaatt ggcgaaacgt
     3601 ctttatgaac aaacgtactg gtgaaaaact tttttctttt gctaattctt ctgtcatacc
     3661 tatcatcacg atgcaacctg gaccaataaa aagtaaagaa aaaataaagg aaaagacatt
     3721 ccttccatct atcgacaaaa ggaacgcagc tgctatatac aagatattga cacaccaaaa
     3781 acaaaggcta cttgccaatg attgatacaa ccaataaaag aattccatat aaacattttt
     3841 gtcaaaacgc tgctctttca taatcacctc ctattccttc attcctgatg aagcgatccc
     3901 ttctacaaaa tatttttgca tcgctaaaaa tataaaagca acaggaagaa cagaaacaac
     3961 tgaggcagcc atgattaatg cgtactctgc atcaaatagc ccaataaaca ttttcaaccc
     4021 taactggatt gttttatttt ccgtagaaga caaataaatg aatggcccca tataatcgtt
     4081 ccatgtatta acgaatgtga tgatcgtcaa actggcaatc gccggttttg taagaggtaa
     4141 aatgatcttg cgatagattc cccactctga taagccatcg atccgcgcac tctcacataa
     4201 agaatctggg atactactgt agtattgctt caacaagaac acaccaaaag cattgaacgc
     4261 ttgtaatagc accaacgacc acaacgtata tgttaaaccc acttttctca tcataatgaa
     4321 ctgcgggatc atataagact gccaagggac agcaatcgtg ccgatatatg ctaaaaataa
     4381 aatatcgcgc cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa
     4441 caactgaatc aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa
     4501 aggaatacga gaccaaataa ccagataatt ctctaaatgc caagttttag ggatccattg
     4561 aatcggaatc gtgaacactt cattatttgc tttgaatgaa gcagaaacca tccataaaaa
     4621 aggaatgatc gtcaataaag ctaatacaat cagcagaatc atacccaacc attgaataaa
     4681 gggcatgttt tttgaacgaa tttttgcttt ttttctttca tttcgaacac ctccaaagtt
     4741 ataatcttca tttctttgta atgattacaa tccagaacaa cggttattat agctgattca
     4801 tttccgttct tcttatgttt gtaagcgctt atttaacaag ttatgtaaat ctctttattt
     4861 tttttgattt aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc gcttttgtat
     4921 tgaaatttct tcaattagag atacttatta ctgttcaaaa gcttctttga aacggtgaat
     4981 caattcattg actacaggtg ataaaatccg attctttttc cagactagca tgcagttcgt
     5041 ttcaatcatt gggtcaagcg gaacgaatgt cactcgcttt gaaagatgac ttacagcacc
     5101 ttctatgact aaagcagcac ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa
     5161 attgtacgtt cctgtgatca gcaactgctc cttttttatg cctaaccagt cagcaaacat
     5221 tcgctgtatt tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt
     5281 tatcacgtct ttttttgcta aaacagaatt ttctaaaatg agcaatcccc atttttcctt
     5341 tcttggtaac aaaatcgttt cgtatttctc tgtatctact ggatctagca aaatcgctaa
     5401 atctaacagg cttttttcta gtcactccgt gatatcatca cttgttccgc tgtggatatg
     5461 aaattgaacg ctgggataat cacggatcaa cttttctagt aatagtgcca atgtattcga
     5521 attatcagct tccacacagc cgatcgtaaa tcggccgctg aataattgtt tttttctgtt
     5581 ctcgaattct tgagtggttt gattcgttaa atctaaaatt tcttctgctc ggctttttta
     5641 gaaaataacc tgcttctgtc aagatcattt tgcggttttc gcgtacaaac agttctgttc
     5701 ctagttcctc ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa
     5761 cttctgcggc ttgagagatt gttccttctt ctgcaattgt ccaaaaatat tttagtaagc
     5821 gtaactccat agcgatgctc cttgattttt ttacttgata cattccattt tatttctaga
     5881 taacacacga tcatatttct tgatttttcc ttgtaaacgt tccaaacact cttgcatttc
     5941 agcaatttct ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc gctcaaatac
     6001 agttgcttct ccttcgctag ataggcgaat atattcaatc aaagactcga tcgataatcc
     6061 ggcatttcgc atacagatcg taaagtcgac ccattttagg tcttcttctg tataatcacg
     6121 gattccgcct tttgtccgat taactggtgg aatcaacccg attcgttcat aatagcgcaa
     6181 tgtatcggcc gaaatgccct tttcttcact gacttgtttg atattcatct gatcactctt
     6241 tctcttaatt tcttgccact ttacttatgt attttattta ttctacaact ttaagtgtac
     6301 tccaagtcaa tactcatttc tattaaaact aagctggatt atctatatcg gttgtttgtg
     6361 tattatttca aataaaaaag tcttgttttt ttattctttt ttggatatac ttttaacaat
     6421 ttagggaaat tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa aggtattatt
     6481 atcgacatcg gcgggaattg ctttagagaa tatggatatt atgtttttag ctttttcatt
     6541 atcttcaatg attacttctt ttcatattag tggtacgcaa gcgggtttca tttcaacgat
     6601 cactaatttg gggatgctca ttggaggtat tttttttggc atcatagctg ataaatatgg
     6661 acgtgtgaaa gtattttctc aaacggtcct tctgttttcg attgcttctt tattgatgta
     6721 ttttgcttca aacatctact tggtttatct ctttcgcttt atcgcaggtg taggagcggg
     6781 aggagaatac ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg
     6841 acgagcttct tcggttgctg gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt
     6901 agctgctgtg atcattccaa cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc
     6961 ggtgttgatg gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt
     7021 acaatctgcc gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac atcttgcttg
     7081 gcaaacatgt ggccttagta tcatggtaac tgtccaaatt gccggctatt ttgggttaat
     7141 gaattggctg ccttcaatta tgcaaaatcg attagggctt tcggtttctg ggtcttcatt
     7201 gtggatggtc agtactattt taggcatgtc tttggggatg atgacttttg gcataatcat
     7261 ggataaattg gggcctaaac tagccttttc tttgttttta atttgttcat ccttatctgt
     7321 ctatctactt gtgttagccc atagccagtg gagtttggta gcagccgctg tgatagtcgg
     7381 ctactttatc aatggcatgt atggtggata tggcgcagtg attagtagtt tgtatccaac
     7441 agaaatccgc gcgacggcga ataatttcat tatgaacctt ggacgagctg ttggtggttt
     7501 ttcttctatc gtcatcggtt tcttgatgga tcattatagt ttaactgccg taatcttgtt
     7561 tttgagcggt atttacctag tcagtctgct ggtcttgaca actcttactg gtgttacagc
     7621 attaagggaa agcttgcagc attgatagtt gagtctgtaa atgacaagaa agaggtgtgg
     7681 caaaagcttg catctgcggt aataagacca gccaaatcaa aaacatgaga cactgtttcc
     7741 gtttggctgg tcgtattact tgatgcaggg cagctttttc aactgctaat ttccttctat
     7801 ctggcggcga ctattctttg acgtccgtca tttcaaagta ctttactaat aacttagcat
     7861 aacgaaaaac tgtggataca ctcgtatatt gagcgtctgc ataatcttcc aaatttttct
     7921 caggatgttg atacatgaat tcaatcaaat gataattata tgattttctg tagaaatgaa
     7981 gataaataat gtagattggg aagttctcct gcttttccat agaaaagcgc cgattattct
     8041 cattcttcaa aatgacctta tcaggatatt tttggaacaa ttcttctaaa tctgttagat
     8101 aattcaagat agacttactt gataccttca attcgttttg tagttcttct attgaacacc
     8161 aacgttctgt ttcaagcagt aaatgaatca ttttaatttt cttttgagaa acttttgtta
     8221 aaaaagtctg catattttct cctcctgtat ctactttact atactacata caagaaaaaa
     8281 aacggataca aatatcatat tgtcacaccg tcaatagtgc ataaacctgt tattatttct
     8341 ctttttttcc taagtattct attgatatga atcatttaat cgtttattat ataaatgtca
     8401 ataatctaat aataaaataa cgtacatatt ttattattgc atacttaata acagaactat
     8461 agcaaaaaat cccgtgtatc aacacgggat tagattaagt ttcctcaatc actattcata
     8521 acacaattat tataacaagt aagtaaacaa ttgaaaagga catttattga aaacggttgt
     8581 tttttactta aggaggaaat ttagcgaaaa aattagttgc attgtttttg gtcgcaggaa
     8641 gtttgttgag tgggtgtggt gctactacgt ctactactca aagcacaagt tcagatcagg
     8701 cttccagcag cagtaccacg atctccgaaa gtacggcaga ttcatcgaaa gaacaagaac
     8761 aaattgaaaa gaaaaaagaa gagaaacaaa aagcggaaaa agctgcacta gaaaaaaagt
     8821 gacagaagcg gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc
     8881 aactgcttca gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct
     8941 tgctgcaatt gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga
     9001 agaaattgca cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc
     9061 agaacaggcg aggcaagaag caatcgcaca gcaacaagca gaacaggcgc gacagcaatc
     9121 agcagctgaa caggcctctc agcaggcaca agccgcagct cctgagcaaa ataatgaaca
     9181 aactgtctat gtcacaccga ccggttcaaa ataccataca cataaatgtg gaaatggtac
     9241 gtatacccct gccactttaa gcgaagctca agctcgtggt ttaacccctt gctccaaatg
     9301 ttatggaaac taattcaaaa gttatactat aataataaaa ttcttctgaa acataaaaaa
     9361 atagaagatg gcgttccaga tagtaaacca aaaataacag tttacacgtc catttcgtca
     9421 gcttctctct tttttcattt agtctgttcc ctctgctttt ttggacatct tcattcattc
     9481 tttctcaatc aaatagtaca cctctttttc ttttcgcagt ttatatcttc cttttttcaa
     9541 tttcattgaa aactgtttcg aatgtttatg ttttacatgg attttaagca ggagctcctc
     9601 atgttgcaag cgatagttcc tgccttttaa atcagaaaat ctcactagtc cgtattcatc
     9661 agaagctttc acaaatggaa ctttttctct agtaatatag tgtttgccgt tgagcgtttt
     9721 tacagaaaag accattcctt cgattggtcg tccgtttgtg ttctttacat aaaaagataa
     9781 aggatagttt cgcttagcga tggccaaaga atctaaataa gcgatgagta gccaaactaa
     9841 gccgattgat ccaagtagta taccgataaa ccaaataaaa aatttccatt ttttccattg
     9901 atcaattcct tggatcgctt tttttgcctc ttttgcttga taaggaatac gatgccccgt
     9961 cacaagcagt cgatgtgtat tgaccatata aggagtacag gtaagcaaag tcacgagatc
    10021 tctgcctttt tggataccca aagaatctac ttcatcaggt aagaccactt ggattttttc
    10081 tacttgatac gccaatgtct ttccattgat ctggatgaaa aactggtctc cttttttcaa
    10141 cttaggcaaa tctgtaaata attttgcttc tgggagtcca cgatgcccag aaagaacggc
    10201 atgggtgttt ttcccgccag tcggataaga actttcttcc aataatgacg ttcctttttg
    10261 caaaaatatt tccgttgtct gatcgaatat aggtaagctg acattgattt ttgggatttc
    10321 gatgactcca agcatatgtt ccataaaaaa ggcattacta ggtaaatctt ttaatgcttt
    10381 tgcatcaact gcttggttga aactgcctat ccctggtgat acgttttgct ctgccaactg
    10441 ttggtttttc tttgtcattt tttcctgctg tttttttatt tcctctgaat tcttattcga
    10501 tgcttgcttt tgataatgga tgatcatttg ctgggcaaga aaatcattca gtgaatcttc
    10561 tacaaaagga taagaaaaga tcccgatccc aacgactaaa atgattgcca tgaagatgtc
    10621 cagtattctt attctgcttt tattcatcat gtccacctat tttctaatat gtatatgaag
    10681 aggttgtgaa atcagcgcct tgacctgagt attagcgaag caaacggcaa aatagttcct
    10741 cctattttgc cgtttgttga gcgaatacca agagaccgac cacttgtcac aacctttctc
    10801 cccatttgaa tcactatcgt ttatttatcg tatgattttt gtttccaata gtttaagctt
    10861 ctgtttcttg atggcgtttt ttgaaataga tcactgctcc tccaactaaa gcagtaccta
    10921 ccaagatgaa tacgatgatc cccataccac cagttgaagg caagattcct ttaggttggt
    10981 tatcgatcgt tgtttcatca gaagttgttg gatcatcatc tactgtaaat tcagtgtacg
    11041 cagcttcatt cttaggtaat gcgtagcctt ctggtgcttt tgtttcttga aggcagtatg
    11101 ccccttgttt cagcccttcg attgaaaatt tcccttctga atcagaaatc aatttcactt
    11161 cttcccagtc agaacctgtg acttcatctg accaagcata tgggtattct cctgaggcaa
    11221 ggtttgctat ttttttcatt aatttaacag ttggatcttt tgctcttaga atagaaaatt
    11281 ctgcatcggc taatttttct tgcgtgttca catcttgttt gatgaagtgg aaatcaccgt
    11341 agttttggac attttctaca gtaatcgtgt tatccgtagt tgtattttcc cgattgacta
    11401 caccgtttgg attcccaaga ttggcttcat ctgcgccatg gatgataccg gttgtttgtt
    11461 gcaattttcc gttttttact ataaaagtag tttctttttg tggattggtt aattgataac
    11521 catctggtgc ttttgtttca actaaagtat aattgccgtc tattaatcca ttgacactga
    11581 tggatccttc agtatcagtt gtgaatgtca acgctgactc agcaggatca tagagagcga
    11641 caccagtgtt ttcatcaaaa ccggtcacga attgattctc tgaatttttg atctggaatt
    11701 gcgcccctgc taacttatct acccctgaaa agttcccagc tttctggatt ttgatattgc
    11761 tttctttgat gacattttta gcatataggt gaatcgtgtc taaaggtttg tcagggtcta
    11821 gggtaagctt aacacgatat ttggcgacgt ttcgacaccg ggctttggtg tttctacaaa
    11881 gacgtataca gcatctttcc catcagattt acctggaaga gtcaggttag ctaatccttc
    11941 accagaagtc gttacacttg cgaagggtgt agcgtctgct ggaactgctg tgtctttact
    12001 tggatagtac gtagcagtga tgtcatatac gttgaattca acgccgttca acggttcgct
    12061 attcggaaat gtcaattccg ttccgttgtt tatttgattt tctggtgctg aagtgaattt
    12121 caatttatgc aaattaaccg taacattttc tggtgtaggt gatacggctg cagaaacctc
    12181 tgcagttcct aggccgagaa cgaaaggaag cagcatgata gcagttccta aaaacgtcca
    12241 tatttttgtt ttgatgtttt tcattttatt tccctcctat tgtttccttt ttgttgtttt
    12301 ttataaaacc a
//
LOCUS       DASJON010000079        12219 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJON010000079 DASJON010000000
VERSION     DASJON010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.079"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..297)
                     /locus_tag="U3989_001587"
     CDS             complement(133..297)
                     /locus_tag="U3989_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715039.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="U3989_001588"
     CDS             592..1920
                     /locus_tag="U3989_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN1715040.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTVVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="U3989_001589"
     CDS             2122..2955
                     /locus_tag="U3989_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN1715041.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="U3989_001590"
     CDS             2971..3708
                     /locus_tag="U3989_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN1715042.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="U3989_001591"
     CDS             complement(3863..4831)
                     /locus_tag="U3989_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN1715043.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="U3989_001592"
     CDS             5063..5896
                     /locus_tag="U3989_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN1715044.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="U3989_001593"
     CDS             5930..6769
                     /locus_tag="U3989_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1715045.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="U3989_001594"
     CDS             6795..7355
                     /locus_tag="U3989_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1715046.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="U3989_001595"
     CDS             7554..9275
                     /locus_tag="U3989_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN1715047.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="U3989_001596"
     CDS             9440..10582
                     /locus_tag="U3989_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1715048.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="U3989_001597"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="U3989_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN1715049.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4172 a         2053 c         2533 g         3461 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccgttgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt gcgaaaaagc
    12181 tgccctgcat caagtaataa gaacagccaa acaaaaata
//
LOCUS       DASJON010000080        12160 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJON010000080 DASJON010000000
VERSION     DASJON010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.080"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(66..1082)
                     /gene="tsaD"
                     /locus_tag="U3989_001598"
     CDS             complement(66..1082)
                     /gene="tsaD"
                     /locus_tag="U3989_001598"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN1715050.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1079..1543)
                     /gene="rimI"
                     /locus_tag="U3989_001599"
     CDS             complement(1079..1543)
                     /gene="rimI"
                     /locus_tag="U3989_001599"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1715051.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1550..2092)
                     /gene="rimI"
                     /locus_tag="U3989_001600"
     CDS             complement(1550..2092)
                     /gene="rimI"
                     /locus_tag="U3989_001600"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1715052.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2076..2798)
                     /gene="tsaB"
                     /locus_tag="U3989_001601"
     CDS             complement(2076..2798)
                     /gene="tsaB"
                     /locus_tag="U3989_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN1715053.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(2989..3969)
                     /locus_tag="U3989_001602"
     CDS             complement(2989..3969)
                     /locus_tag="U3989_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1715054.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(3993..5477)
                     /gene="galT"
                     /locus_tag="U3989_001603"
     CDS             complement(3993..5477)
                     /gene="galT"
                     /locus_tag="U3989_001603"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN1715055.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5489..6478)
                     /gene="galE"
                     /locus_tag="U3989_001604"
     CDS             complement(5489..6478)
                     /gene="galE"
                     /locus_tag="U3989_001604"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN1715056.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            6726..6893
                     /locus_tag="U3989_001605"
     CDS             6726..6893
                     /locus_tag="U3989_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN1715057.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(6955..8766)
                     /locus_tag="U3989_001606"
     CDS             complement(6955..8766)
                     /locus_tag="U3989_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN1715058.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(8763..9128)
                     /locus_tag="U3989_001607"
     CDS             complement(8763..9128)
                     /locus_tag="U3989_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN1715059.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9291..9686)
                     /locus_tag="U3989_001608"
     CDS             complement(9291..9686)
                     /locus_tag="U3989_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1715060.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(9704..10666)
                     /locus_tag="U3989_001609"
     CDS             complement(9704..10666)
                     /locus_tag="U3989_001609"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN1715061.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(10666..10878)
                     /locus_tag="U3989_001610"
     CDS             complement(10666..10878)
                     /locus_tag="U3989_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN1715062.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(10899..11597)
                     /locus_tag="U3989_001611"
     CDS             complement(10899..11597)
                     /locus_tag="U3989_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN1715063.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(11617..12159)
                     /locus_tag="U3989_001612"
     CDS             complement(11617..12159)
                     /locus_tag="U3989_001612"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN1715064.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
BASE COUNT         3395 a         2685 c         2078 g         4002 t
ORIGIN      
        1 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc tttttcacaa
       61 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
      121 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
      181 cgaagagggg gaatcactag ttctacctca gggagtttag ttgccaaagc atcctgcaat
      241 ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg cttgactgga
      301 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
      361 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
      421 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
      481 gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
      541 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
      601 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
      661 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
      721 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
      781 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
      841 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
      901 cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac gccaccaaaa
      961 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
     1021 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
     1081 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
     1141 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
     1201 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
     1261 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
     1321 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
     1381 ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
     1441 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
     1501 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
     1561 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
     1621 tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga ttactcatac
     1681 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
     1741 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
     1801 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
     1861 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
     1921 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
     1981 tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt tctgtataaa
     2041 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
     2101 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt taaatagcga
     2161 ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg agccacagtt
     2221 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
     2281 tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
     2341 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacagtttct
     2401 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
     2461 atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt cagactagat
     2521 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
     2581 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
     2641 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
     2701 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
     2761 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
     2821 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
     2881 aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
     2941 acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt atttcgttga
     3001 ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt
     3061 acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac
     3121 agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc ccatcacaga
     3181 aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa
     3241 tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatactgctt
     3301 cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta
     3361 gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg
     3421 gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac
     3481 aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc
     3541 ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa
     3601 taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa gatcactaat
     3661 ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc
     3721 taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat
     3781 caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg
     3841 ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata
     3901 gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat
     3961 tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac
     4021 gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa
     4081 acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt
     4141 tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct
     4201 aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc
     4261 cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga
     4321 tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac
     4381 tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct
     4441 gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt ttccaatact
     4501 gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat cacagacatc
     4561 ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat aagtttttct
     4621 atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga
     4681 atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg aggcaagact
     4741 tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt
     4801 tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg gaatccccag
     4861 ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag
     4921 cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc
     4981 gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg
     5041 atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt
     5101 gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg
     5161 cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa
     5221 tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt
     5281 tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt
     5341 acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat
     5401 aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc
     5461 gtttgactga ttgacatatc tcattcccct attcttcata accatttgga tggctgacat
     5521 gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt ttccagccca
     5581 gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg
     5641 gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct
     5701 tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca
     5761 agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga
     5821 tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct tttctttgac
     5881 caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga
     5941 tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc
     6001 catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg
     6061 gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc tctccataag
     6121 ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg acttcaagta
     6181 agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc
     6241 ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg
     6301 aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg
     6361 cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta
     6421 gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa
     6481 aaaacactcc tttgattagt aaaattgtgt ttgttgattc tatataattt aaaaaacgaa
     6541 gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta
     6601 taataaaaca tttagtaaac attttcaagg attttttgca aaggtttaca aacaaacgta
     6661 aaactagcct ttttttctct tttgttccat aatagatata acaaataata aatgaggtgg
     6721 attcaatgaa aaaatttgta tctggaatat tagtaggaag tctggcaaca gccgctgccg
     6781 ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag
     6841 caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat
     6901 ttaaaaatac gcatgaagac tttctaaaaa acagataagt catcatgcgt atttttattt
     6961 gtcagtcaat ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata
     7021 aggtctagct actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata
     7081 aaagtcagac tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc
     7141 tatagcttct gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt
     7201 gttttccttc aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa
     7261 tcgagcttca atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg
     7321 gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat
     7381 cagtgctgct aattctttcg tccgaatgat tggcggaccg atcgttaaaa agctatttag
     7441 atgagtaaac catccccata gtatttcttt gtattcctta gcagaaaaaa cgtgccattc
     7501 aatagcctgc cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc
     7561 tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct tcaataaata ttcggctagc
     7621 ttctgaaaag ggctcattct gataggcaag aggagtttct aaatacgacc aatcccatct
     7681 ataagggatt agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc
     7741 atgctcaaaa tttttctttg gaatcggcaa gtgatattct tcgatgattt tcttgtaata
     7801 aaacaactca acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga
     7861 ctgatgccat tgctccaggt tttcatttgt tagtaaccgt ggccatgcca ttgctccgcc
     7921 ttgcttttga tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac
     7981 aatcggttcc ttgcctgatg gatgatagac cagttttccc ttttcgttct cgaagcgccc
     8041 tcctcttgat actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa
     8101 aaagacattt tctttaggcg gtatcttcga aagatctaca tctgcaggat tagaaggaag
     8161 ctgatacagt aaaacatttt tctttgcgta gtctacccac actctttctt cttctgtctc
     8221 atcattttcc cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc
     8281 tgtctgtaaa agataaccat cttttctttt gcttactttt tttactaaag aataaacaaa
     8341 ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc
     8401 gtataaacat cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag
     8461 ttgcttattt ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg
     8521 aaacaagttg ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag
     8581 cgtcacttgt tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac
     8641 tcgacctcca ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt
     8701 attatggaga atcctttctg ctacacttaa tcctctaggt ccggcaccaa tgatccctat
     8761 tttcatttgc atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt
     8821 tcattttttg caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt
     8881 ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca
     8941 aatgaagcaa atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca
     9001 atttctttca accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaat
     9061 ctatccacga gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat
     9121 ttgatcatag ttgtttcctt ctttctacct tttcgcttag cttatatatg tagataatag
     9181 cacaaaataa aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc
     9241 aaaatctaat gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt
     9301 ttacttttga tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga
     9361 taacctgctt tacgtaattt atttgctgta cggatgctca aacttttctt ttgatcgtaa
     9421 atatagacag gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg
     9481 atgtttcttg cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt
     9541 acttgtgctt ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt
     9601 ttggccatta ctcgtagata gacttcccaa aggatgattg caagcacaat caatagcaaa
     9661 atgatattaa ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg
     9721 ctaatgaggc agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg
     9781 ttgattctct tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta
     9841 aaaattctcc tgctgccgat actcctccac ctaacacgat acttgacgga tttaatgtat
     9901 tccccaagtt tccgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg
     9961 caaacggatc gtcttcttga gccatttcaa agatatcttt acttgttact tcttctccgt
    10021 tgtctagcat tgctttcagt tttgaatccc ctgcgtattc ttctgccaag aaacgaccta
    10081 ggcgtaccac acctgtagcg ctagatacag tttctaaaca tccgcgttta ccgcaagtac
    10141 attcgaaccc ttcaggatcc acagtgatat gtccgatttc tcctccagct ccgctcacgc
    10201 cgtgaatcaa atttccgcca gcaacgatac ctccgccaac acctgttcct agagtaacga
    10261 atacaacgtc tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat
    10321 tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa
    10381 attgaacttc tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca
    10441 cgcttcctgg ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat
    10501 tcaaacgatg attgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa
    10561 tattggtatc gatgctccat ttttgctgaa tctctccttc tggtgttaaa attgcaaatt
    10621 ttgctgttgt tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt
    10681 ctttcggctt tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt
    10741 atcatgatca atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat
    10801 gtcatagatg cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac
    10861 atcatataag ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt
    10921 tttaaaccca gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc
    10981 ctttggtgta tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact
    11041 aatccgccaa ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca
    11101 aaaacaaatg tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg
    11161 ttatgtttaa aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta
    11221 cttgcaccac cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt
    11281 atcccgctga acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc
    11341 tgccccataa aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg
    11401 aagattggtg tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt
    11461 ccggcataat aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc
    11521 tgtatcccta aaaaagcata ggtccataac ggtcgtctta accatacacg caattttatt
    11581 tgctgttgat aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat
    11641 cttctgatca aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct
    11701 acatgttttt ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat
    11761 tcgataaccg gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg
    11821 caaatagagc ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga
    11881 ctgttcatcc atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat
    11941 tttcttttgt tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt
    12001 cgataaagtc gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt
    12061 atagattcgc tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc
    12121 gattcctttc cggcgtaatc ttactttcaa ttggtccaaa
//
LOCUS       DASJON010000081        12082 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJON010000081 DASJON010000000
VERSION     DASJON010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.081"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..192
                     /locus_tag="U3989_001613"
     CDS             <1..192
                     /locus_tag="U3989_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1715065.1"
                     /translation="FLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQE
                     LKMTILDEVHVVNEEEDFF"
     gene            170..697
                     /locus_tag="U3989_001614"
     CDS             170..697
                     /locus_tag="U3989_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715066.1"
                     /translation="MKRKIFFRRKKKIKAVRIDERLIHGQVAMTWTKSLNLTGLVVAS
                     DEAATNDIQKMTLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKV
                     AKAFPTEIELMNVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPT
                     PAMEKRSAISILSDF"
     gene            718..1527
                     /locus_tag="U3989_001615"
     CDS             718..1527
                     /locus_tag="U3989_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715067.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            1524..2345
                     /locus_tag="U3989_001616"
     CDS             1524..2345
                     /locus_tag="U3989_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1715068.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            2396..4495
                     /locus_tag="U3989_001617"
     CDS             2396..4495
                     /locus_tag="U3989_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN1715069.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            4492..5610
                     /locus_tag="U3989_001618"
     CDS             4492..5610
                     /locus_tag="U3989_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN1715070.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            5649..6527
                     /locus_tag="U3989_001619"
     CDS             5649..6527
                     /locus_tag="U3989_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1715071.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            6718..6870
                     /locus_tag="U3989_001620"
     CDS             6718..6870
                     /locus_tag="U3989_001620"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1715072.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7894..8706)
                     /locus_tag="U3989_001621"
     CDS             complement(7894..8706)
                     /locus_tag="U3989_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715073.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            8869..8976
                     /locus_tag="U3989_001622"
     CDS             8869..8976
                     /locus_tag="U3989_001622"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715074.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            9034..10101
                     /locus_tag="U3989_001623"
     CDS             9034..10101
                     /locus_tag="U3989_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1715075.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            10243..10446
                     /locus_tag="U3989_001624"
     CDS             10243..10446
                     /locus_tag="U3989_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN1715076.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            10506..11708
                     /locus_tag="U3989_001625"
     CDS             10506..11708
                     /locus_tag="U3989_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1715077.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         3778 a         1969 c         2636 g         3699 t
ORIGIN      
        1 tttttggaga aagagattgc tgtaactatt ttgtctggct ttaatctgcc aattctgctc
       61 gaaatagtat tggcaaccaa ggaattaaca aaggaggata ttcaggaact ggtaaataaa
      121 tgccaacagg aattaaagat gaccattctt gatgaggtac atgtagtaaa tgaagaggaa
      181 gatttttttt aggaggaaaa aaaagattaa agcagtgcga attgatgaac gattaattca
      241 tggacaagta gcgatgactt ggacaaagag tctaaattta accgggttag tagtagcaag
      301 cgatgaggcg gctaccaacg atattcaaaa gatgacattg aagatggctg caccatcgaa
      361 tgtcaaagtc attattaaaa cagttgatga cgcgattcat ttgttaaaag atccgcgggc
      421 gaaggacatg cgcttgatgg ttttagtccc taatgtcaaa gatgcggtaa aagttgcgaa
      481 agctttccca acggaaatcg aattaatgaa cgtggggaat gctgggaaaa tgacaccgcc
      541 atcggaagat aagaaaacct tatcaaagga agtgatgtta acgacgtcag aaattcaaga
      601 cttaaaagaa ttagttgaac tttatccaga taccgttttt cagcctacac cggcgatgga
      661 aaaacgttca gctatctcta ttttgagtga tttttagaaa aaggaaggag aaaaattatg
      721 tatgatgcat ttgtagtcgc tttggccgtt tttattggag ttgctggaca tgaaatgttc
      781 gggatgtcca tgctgagtcg tccgattgtt gttgctccgt tagcaggagc gttgatgggg
      841 gatttgcaga cggggttgat tgtcggggcg tcactagaag ccatctttat gggtgtcgtc
      901 aatatcggaa tctctagtac ggcggagcct tctttggctg ctgggctggc tacagcgttc
      961 gcaatcaaaa tgggaggaaa cgtggatgcg attattccca ttgtctttcc actagcagta
     1021 ttaggattac agttaatgaa tatgattttt tcttttgtat gtggaccgat ggctgctaaa
     1081 tttttcaaat ttgcaaaatc tggtgatcaa aaaggaatca tcaatctgca cttctttatg
     1141 tggtttgtcc actatgcgct ttatgctctt attccattct ttgccgtttt atttggagca
     1201 gatgttgtca aaagcgtctt ggataatatt ccagaggtca tcatgaacgg attaacagtc
     1261 gctggaaatc tgctgccggc agttgggatg gcgatgctgt tacggatgct ttggagtaaa
     1321 aatattgctg tgtggttttt ctttggggcc gttgttatgg cgtatctcga gttaccattg
     1381 attgcaattg ccgcagttgg aacgattatt gcaattgtta tagcccaaag agatctacaa
     1441 ctaaaaaata tatctgttca tgcagttcaa agcgatgctg ctgtaaatgc aacgcttgac
     1501 caagaagagg aggatttctt tcaatgaaat tagctgatgg aatttctaaa gatgagaaaa
     1561 aattggttcg gaagatgttt tggcgctcga tgactatgta tgcttcggtg aatccggtga
     1621 cgatgggcgg cggtggtttc gcctattcta tgatgccctt cattaatcat ttctacaaaa
     1681 gtgaagaaga tcgagctgcg gcgctggaac gtcatacaca gtatttttcg acgaccattc
     1741 cttgcgcgtc ctttgtcatg gggattgcgg catcgatgga gaaggaaaat tctgaaaaag
     1801 ctggttttga cgtggaaagt atcaacgcga tcaaaacgag tttgatggga ccattggctg
     1861 gaattggcga ttcattgttc tggggagttt ggcgtgtcgt taccgcggga ctagcgatca
     1921 acttagccaa tgccggaaat attttggcac caattatctt tttagttatg ttcaatatac
     1981 ctaattactt gatgcgatac tatggcggcg ttttaggcta ttcattaggc tctaaatata
     2041 ttgaaaagct gtatgcgaac gggttaattg aaattctgac aaaagctgct agtatcttgg
     2101 gtctgatcat ggtaggtgca atgacgatca gtatggtcaa attcaatacc gttttgagct
     2161 tttcaatgaa gggcgaagaa ctaatgaatg tccaagaaat cctcgatagt atttttaaag
     2221 gaattgtacc gttattggta acattgagct gtttcaaatt actagataaa aaagtcggcg
     2281 tcatcaaaat tatttttgga atggtcgtct tgggaatttt attgtcatta cttggaatcg
     2341 catagtagga agctgcagct actaggctgc agttttttta gcaaggaggg aaaagatgac
     2401 tattaatgaa gtaatttatt cacccaaaca cccatttttt aatgctgagt taaacgacgg
     2461 aaatgtgact acttcaaacg aatacagcta ctatcggaat attcctgatg taaagggatt
     2521 gaccttcgat gtaagtaatc aaaagacatt agcaaacctt gatgtttgcg ggacgattca
     2581 aacgttgacc ttttatcgag aaaatttact aactgaggaa aaaccaggtg tttgggtcaa
     2641 taaacagctc tctcaaacca attcgttatc cttaaaggtt gaagtaaacg ggattgtcta
     2701 ccatttgtca gaatcggatc atcgtattga agtcgatgtg attcaagact gtttgcctag
     2761 atatattcat cattattcga cgtttaaggt cattgtagta tcctttgctc ccatactggc
     2821 taataagcgt ttaagtatgc ttgttcaaca ggtttacatt gtgaatgaaa cacacgaaag
     2881 ccttcaagtt tcagtgaaag atgtgccttc gtatcaagag aagtattcgg atcagcaaaa
     2941 tgtgttgatt tctcaaaaag gaaataagga cacgatcgca caacaggaag tggcaagctt
     3001 cgccattgct tttgtggacc caaatgcttt tgaagaagta atgacttttc aggaatctga
     3061 tatcgaaaaa tggctgaagg aaacattgaa ctattttgct cagctatttg ggcagctgtc
     3121 aatgccaaac gatagagttg tccatctcta tcgtcgtgca ctttatcaaa gcttttctag
     3181 tttcggtatg aatcgacagg gacaggtcgt cggcagcaat tggggcagct atcctgctac
     3241 taatcgaatt tggaataagg atatgtatta cgcatcattg ccattcacca tgtttgaacc
     3301 ggagttatgt caaaaaacgg ttctatggtt tgatcaatat ggcgttaagt ttcctggttc
     3361 aaaatttaca ggaggtatca atcattctct gagtaattcg ttatccagtg cgttactggc
     3421 tagtctttat tttgaacata ctggggatac aaaattcttt gagcggtgtc cgcaggttct
     3481 actgaatgct tcaagaatta ttgaggacat attagctcaa cgtcggtcag gggaaccaat
     3541 gctttttcca tctgtttggt tatcagacgc ctttgcactt ggaaagtatc ataccggcag
     3601 caatctttgt ctttggaagg cctgtattgg acttgcagaa atttttgaag ccctagaaca
     3661 actgagtctt agcaagcgat acaaaaacat tgcctgtaaa ctaaaggaat caatttgtaa
     3721 acaaatgact atcgatggtc cttttggtga gcaatttttg gagggaatcg gtgatgagga
     3781 gaaaacaacc tattcagtga agcactatca aaaaccgatt cttgagcaag ggttaatctt
     3841 tttaagtgat gtgattgtgg atggaaaaat taatttattg atgcatgatg gagaagaatc
     3901 agacactacg ttgatgcctt tttatcaatt tttagataat aaagatcatt tatatcagaa
     3961 caccatggcc ttttctgcta gttctttcaa tccaacctat agcgaggaaa tcaaaggcat
     4021 cacttgggga ttagaatccg gcgccacttt tcctggcttt atcactgttt taatgagcga
     4081 gctgcacaac cctcaagctt tcgtaacccg attagaagaa ctaatttgtt tggcagattt
     4141 agacggttcg tggtggtggt ggccgtatcg cttagaggct caacaaggag aagtcgtgcg
     4201 agactttggc tgcggcaaat gcggctgggc atcgggtctt tttgtcagcc taatgatcag
     4261 tcaatatttc ggattgaaat tcaaaaaagg agtattgcaa attgatcctg ttgataattt
     4321 aacgttttct tggaagaatt tgaagttcgg tcaagccttc atttcgattg aatgtacgca
     4381 atccgagttg agtatttcaa atttgtcaga aaagccattg aaaataagtg atacgaaaaa
     4441 aattcttcac gtagaaaagg gaaaaacgat tcacattcaa aggagaaaaa gatgagtata
     4501 ctaaaaaata ttacccaatt taaaaaaatc cagagtcgag caatcactcc cgaaaatcca
     4561 acaggcgaga aaggtaaagc tgcgatgttg ccaagcgagc taggaccaag ccgcaaaggt
     4621 cgtggcgcaa ttccgctgac ttctggtgaa gaaactgtga ttgcggatat tactggaacg
     4681 ggaataattc gtcatatgtg gatgacgatt cgtgatcgaa cagaatttgg cagttttgtt
     4741 ttacgtgatt taatcttaag aatttattgg gatgggtcgg atagccctgc tgttgaaagt
     4801 cctttagggg atttcttttg taatgggttt ggagagcgtt gcgaggtgaa ctcgttgccg
     4861 atagtggtta atcctacagg gggctttaat tcctatttcg aaatgccttt ccaaacatcc
     4921 gctaaaatta cgatcgagaa tttacatccg aaggatatgt cctcattttt ttatacgata
     4981 aattacgctt tggtggacga attggaagaa aatagcctat attttcacgc aaattggcat
     5041 cgggaacgca ttaccaaatt acaggaggat tataagttaa ttgatcgatt aaagggtcaa
     5101 ggctattacg tgggtactta ccttgcgctt acctgcctag aacgttattg gtggggcgaa
     5161 ggcgagttta aattctacat tgatggggat agtgagtatc cgaccgtaac ctcaacaggt
     5221 tcagaagatt attttggcgg ggcgtgggcc tttcatgagt acgatgagtt cggacgtcca
     5281 catgcgaaaa actattcaac agccttttta gggtatcctt accaatcaaa tagagatgcc
     5341 acaagagatc gttttgaaac gggaaaattg aatgccgttc atgcctttgg tgacgacgca
     5401 atgccacgac atggtcttta tcgttggcat cttcaagacc ccatctgttt taccgaagat
     5461 ttatcggtca ctttacaaca aatcggcaac gatgatgttc gcttatttga gcggcaagac
     5521 gatgttgcta gtgtcgctta ctggtattca acagaagctt caggaaatac taaacgtcat
     5581 ttcactgatc gagaacgctt gccgcgttaa tgaagtgttt ttcacatatt cgaagggagg
     5641 aacctatgat gatatatggg gcaattgaag ctggagggac taaatttgtc tgtgcagtcg
     5701 gtactgagga attaacgatt ttggaaagag tcagttttcc cacaacaact ccagttgaaa
     5761 caatgaagca ggtcattgat ttttttaaac cttttaagga acaattggca ggcatcgggg
     5821 tcggctgctt tggtccaatt gatattcaat gcaaatcaac gagtttcggt catatcacat
     5881 caacaccaaa acttgcttgg caaaatttca attttgtagg aacgctaaag caatatttca
     5941 atattccgat tgaatggaca acagatgtca atgcggcttg ctatggtgaa tatgttgctg
     6001 gttctggcaa agggttagca ggtgttgcat acttcactgt gggaacaggt atcggaggtg
     6061 gtgccttgtt caatgggact ttcgtggaag ggttcagtca tccggaaatg gggcatatgt
     6121 tgatcaagcg acatccaaag gacaattttt caggaaactg tcccttccac caagattgtt
     6181 tagaggggat ggcagcagga ccagcgattg aaaaacggtt gggtgtaaaa ggccagaatt
     6241 tattggctga cgattcgttt tggcagattg aagcctttta ccttgcccaa tgtgcataca
     6301 atacaacact gatgttttcg ccagatagaa ttatttttgg cggtggtgta atgaaacaag
     6361 aacacatgaa gaaaaaagtt caggataaat ttgttgagct tataaatgga tatgttgaaa
     6421 taccaccaat tgatagctac attattacgc ccgaattagg tgataatgcc ggaataatcg
     6481 gcggattagc attggccaga aaagcggtaa gaaataagca accataatta atgaatgttt
     6541 cattgatact catgtcaaaa gtatttaacc gagtaatcaa atgatcaaat gaagttggat
     6601 aaagcgaaaa tgaattcaaa taaaaaggct ctaagtcaaa tcgtgttgtt ttcattaaat
     6661 cactgaaagg aaatgcttct gcgtttcctt ttttcttttt gttgtgcgct gtataccttg
     6721 aggatattca ctggtggcgt acacttaaat agtaatatat tgtgttactt tatgtctgtg
     6781 gtaatagtta tagttgctta ccaatgtatg ttcatttttc gaaatgttga cttcataatc
     6841 cttggcttaa tgtttgaatt tttttggtga tagatttttt cagccgtata aaataaatcg
     6901 taattctatt atagagagtt gtgattataa aaagatagca agaaaaaaaa agaaagtttc
     6961 tatacatttt aaatatttta ttcctatttc tattgctaaa aaagtagaaa ttctatattt
     7021 acctttaact ctcgcttctc ttgcaaatgt agttagcatt ttaatcgata ataaactcag
     7081 gtagttatat taccttgaaa tgattgataa ttttgtcact tctatttttt ttgcaataaa
     7141 ttttttattt tagtgaaaaa taagaaacta tttctttgaa ggcgataagt tagaaaaata
     7201 ttattgtagt cttttcttta ttaggaatca tcactgttgt atcttattac cttttctgtt
     7261 tttcttatta gagaaaaata atttgagagg tagatagttt ttattggagg aagtaattat
     7321 gtatattttt gaaacaaaaa tttttcattg caataattgc aacaaagaaa tttcatcggg
     7381 ggaagaggtt tggacaaaaa tggaaatttc cgccaaaagt aactgctcct caactgaaat
     7441 caattaaaga actagaattt gagaatgcac tggtaatttg tatgacatgt actgatgaag
     7501 tatttcgaaa acatttttaa aggttgagat gtatatagat ttaaccaatc atttatctct
     7561 ctcggaaaag gaatattaac taaccagcgg gttatcaaaa ataggtagga aaactgtact
     7621 taatggctta tttcgcgctg attatgagaa tatcagtctg tttgattaat ttttagggtt
     7681 caaaatacag agacaaactc caggtcgggt ttcgccacaa gcgaattacc cgatctggag
     7741 ttttgtctat tgcaggaagg cgtagccgga ctgcaggaat aaagcccccg gttctaacag
     7801 ggggctaaca aagaacagga aactgtgaca actacccatt ggggagtgtc ctagcagtat
     7861 tttctttgtc acgtgcttat caattttgtc acctcatttt gatgatggat acgctgcatt
     7921 taacaggcgt tctttctcga cttccatcac gaatggtaga taaagatatt gctgttctag
     7981 tagatattga ttgtcctctg tgtactgata agaagcggtt tcttctgaat agaccaacgg
     8041 ggcaattcgc acatcgctgg tcaattgatt aattgagtat agaaggatgg ctttctcacc
     8101 tagttccttg ggtaaatgtt tcccaaaata ggaataggat ttttgatttg ctaaatagac
     8161 gagtatgcga tccacactaa agttggagaa ggtaaagggc atcatgtgca gaatatgatt
     8221 ttgattatca tgttgcaaat agagctgggc ttccttacca aaattagttg aaccaagata
     8281 aagctgcttt ttttcatcga tacattggaa agtaaactgg tcttgttcag ttggtggaat
     8341 gagatttgta ataaaaaatt caatcattcg atccatttct ttttcactaa caaggtcagc
     8401 gtgtttgatt tcgtctacta tgagaccttc aatcttagga tcaagcttac ctgccttttt
     8461 ttggagcatt tctaaaatgt attgattctc ttgatcacca tacaaaagat attcattggt
     8521 gacatgacct atctctgcga tttggtgaag ggtttcggtt tttggaaggt tgatcccacg
     8581 ttcccagtta ttgactgtac ttcgcggtaa atcaccaatt agtttgccaa atttctccat
     8641 actcaacttt aattcttggc gaatctgccg aattcgattg ccgactgctt gcttgtctat
     8701 ctccatgaaa actcctcctc aggataagtt cttaaattta gtatacgtgt gtacttaaaa
     8761 aaagtcaaaa catattgact taaaataaat acaggcgtac aataaaaatg tacttaaaat
     8821 aagttcaata ggttctttga caagtaaata gcagaaggag gtttgtcgat gaaccaagtt
     8881 aatcaacatg atctcggtga agccattcga gtttctcgag aagaaagggg gtggacacaa
     8941 cgatatttag cagaaaaagt ggggatttcg agatagttat tatcgaaagt agaaaaagga
     9001 acgagacaat taagtgaaga aaaattgaat cttgtgttgg atagtttgca agaagaggtc
     9061 gttccagtta accgtgtttt gattgattat ctaaccattc actttttctc gaatcaacat
     9121 ttaaagctaa ttgaagagat tattgaaatg cccattgaac gttttgagga actggattat
     9181 gcgccaaagg gctatattgg ccaatacgtg tggaatcaag taatcacgat tcggtattcc
     9241 attgacgatg cggttaaagg aactgtgatg gagttttcag gtcaaggctg taaacactta
     9301 gcaagatggc taaaaacacg aaaaacaaat tggcaacagt tttttcgtaa ggtattagat
     9361 tatcagggga actttacacg gattgatttt actttagatg attttgtggg gagtctcagc
     9421 attccagaac ttaagcataa ggtaatacta ggccatgtgt ggaccacttt tcaagtgagt
     9481 gaatctcatg gcggtacgga tatcataaac aatgaatcaa atggtgagac cttgtattta
     9541 ggctcaaaga agagtcagtg tcgtttttgc ttttatcaga aggaccatga acaacggaag
     9601 cgacgaggaa ttcctttaga agaggcaaaa gtaaagaatc gctttgaact acggtatcga
     9661 aaagagaaag cgcaaagctt ggcaaagatt atttcaaaaa cccatgattt aaccaaactg
     9721 ttctttgagt tacttaatgg agcaatttgt ttttatgacc gtgatccaaa tgatctaggt
     9781 gcaaaagttg atgccaaatg ggcagccttt attagcaatt atggcgcaat caccatttct
     9841 ttagatacaa ttcctcaaag ttttgagaaa actatgagtt ggttaattca tggtgtttca
     9901 ccatcattag tttttattgc acaggtcgat caaacgtttc attcttcctt gttgcctttg
     9961 attattgaac aaggagaaat gaatccaaga cagatcaaac ttctccaaaa tatgaaggca
    10021 gacccggagt attatcagga ggaggttgag tattatgaac gaaaattaca aagtatatca
    10081 aatgagaaaa caagctacta aaaaagcaaa gtactgctta cacaacaagt tgtgcaacgt
    10141 actccgcctc gacaagacga gtataacaca accatcgaat gagaaacaat agattataaa
    10201 gaggattatt ttaaaaggag taaagaatca ggaggtgaga gaatggaaac aaaatgtgat
    10261 ttcatggtaa atcgtgcaat tttgatagag atgggcttta aacctagtca agctgcacga
    10321 atgattaaag agagcaaggc atatcttgca aggattgaag gtattgattt ttataataat
    10381 cgccaagttg gtgtggtgcc atcccgagta attgaacatc tttttcacat tcaagttgcg
    10441 gaataaacag tacaagcgcg gtactattaa agtagctttt ctcaagtaca gggaggaatg
    10501 gaacgatgcc aaaaattaaa aatatttacc aagataaaaa gacaaaaaaa tggttcttcc
    10561 gagcgtatct aggtatagat gaaaatggaa gaaaggttca gaagactaaa cgaggttatg
    10621 cttcacaaaa agatgccaaa attgcttatg acaagtacat ggaaacacat gattttaaca
    10681 agacaattcc taatcaactg tgttctacca atcaaatgac tttcgaagag ttttacaagg
    10741 ataggtttgt aaaatggtac gagagacagg tcaaaagtca aacatatgag aatgcgcaat
    10801 tcattttcga aaagcgtatg cagtttttct atcattttcg tgttcgagat attacaagtc
    10861 acgatattga ggattggtta tttgaactaa gccagacaga gtcacgtaat tcacggaaaa
    10921 aagttaatgg cgaaacgtta tcaaaatctt atatcaatcg gattcgaggg cacctaaaaa
    10981 ttgtattgga tcgggctgtt aaagaggggg tgattgctaa aaatcccgtt gaagatgttt
    11041 cttcgcttcc ttctgagaat aagaaagttg agttttggga acacagtgag tttcaaaaag
    11101 tgatgaatga attgaaggat gataagattc aaacccacca tcggaagatt gtgtatgaaa
    11161 tgctctttta tactggcatt cggattggag aacttgaagc tttgtcttgg tcgaatgtag
    11221 attttgagaa aaacacgata acgattgaga agacattgat ttataataca aaagatgatt
    11281 ggtacttttc gactcccaaa actaaatgtg cttatcgaac gattgggttg gggaagaagc
    11341 tttccttaaa actcagacag tggtatgact tgcaaaaacg tattggaaat tttgaatatg
    11401 ttgctcagtt ggacggaaca tttacgccac cttattcttt tgcgaactgg ttaaaagaag
    11461 ctgctaaaaa agcgggtgtc aaaccgatta agcttcacgc gctacgacat tctcatgttg
    11521 catttctaat tgagcagaat attcaaccgc tatctattca agaacgattg ggccatgcga
    11581 atattcaaat cactttagga acttacggtc acttatatgc aaagtcagat caacaagtgg
    11641 taagtgcgat tgataatgtt caagagggcg ctattctttc aaaaaatgac aatttattac
    11701 cagtctgagt tttccacggt tctaccataa gacaaataaa tgcttttgca ccagtgtttt
    11761 cagtcgaatt gctcctgttc aagatgtatc ggaaatagcg agaaaataag caaatttgca
    11821 taactataat aaaaaggact tagttttgat gggaaatttt tcaaaattaa gtcctttttt
    11881 atgtggttga aacgggtatg atcattttaa ggaagtgatc taagatgaaa ggacgactga
    11941 acaattatca tattagaaaa gctttattgt attccacaaa caaattacta agaataacaa
    12001 attagttttt gtttgttctt attccctatt gacttttctt tcaaacatgc tattctaatt
    12061 ttattctaat agtatccgat ga
//
LOCUS       DASJON010000082        12055 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJON010000082 DASJON010000000
VERSION     DASJON010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.082"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..361
                     /locus_tag="U3989_001626"
     CDS             <1..361
                     /locus_tag="U3989_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEN1715078.1"
                     /translation="ARNNKYKGFQQLILDTLEANEIPYQVNGDLTNKLPHVLNLRLIG
                     EANDLLLMKLDLNGVAISTGSACTAGNIEPSHVLEAMYGEDTPILKESIRISFGLGNT
                     EDDIQYFLDQLVRAVKK"
     gene            498..842
                     /locus_tag="U3989_001627"
     CDS             498..842
                     /locus_tag="U3989_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN1715079.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            1113..2399
                     /gene="efmA"
                     /locus_tag="U3989_001628"
     CDS             1113..2399
                     /gene="efmA"
                     /locus_tag="U3989_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN1715080.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(2537..3136)
                     /locus_tag="U3989_001629"
     CDS             complement(2537..3136)
                     /locus_tag="U3989_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN1715081.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            3540..4661
                     /gene="mnmA"
                     /locus_tag="U3989_001630"
     CDS             3540..4661
                     /gene="mnmA"
                     /locus_tag="U3989_001630"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN1715082.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            4907..6328
                     /gene="arcD"
                     /locus_tag="U3989_001631"
     CDS             4907..6328
                     /gene="arcD"
                     /locus_tag="U3989_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN1715083.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            6342..7460
                     /locus_tag="U3989_001632"
     CDS             6342..7460
                     /locus_tag="U3989_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN1715084.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(7520..8812)
                     /locus_tag="U3989_001633"
     CDS             complement(7520..8812)
                     /locus_tag="U3989_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1715085.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            8944..9522
                     /locus_tag="U3989_001634"
     CDS             8944..9522
                     /locus_tag="U3989_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1715086.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            9651..10028
                     /locus_tag="U3989_001635"
     CDS             9651..10028
                     /locus_tag="U3989_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN1715087.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            9961..12033
                     /locus_tag="U3989_001636"
     CDS             9961..12033
                     /locus_tag="U3989_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1715088.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         3923 a         2145 c         2581 g         3406 t
ORIGIN      
        1 agcacgtaac aacaaatata aaggattcca gcaattgatt cttgatacat tagaagcaaa
       61 cgaaatccct tatcaagtaa atggtgattt aacgaataaa ctccctcatg tgttgaatct
      121 tcgcttaatt ggcgaagcaa atgatttgct actgatgaag cttgatctta atggggtcgc
      181 tatttctact ggttcagctt gtacagcagg aaatatcgag ccttcacacg tattagaagc
      241 aatgtatgga gaagatacac cgatactaaa agaatccatc cgtatcagct ttggtttggg
      301 aaataccgaa gacgacatcc agtatttcct tgatcaatta gttcgtgctg tcaaaaaata
      361 actagtgaag tcattcttat atgaacagat gtctgagaga acgccttcat tttttaggga
      421 acttaactga aaaacctagt aaaaatcagg ataaactttt ataataaaga gaaactaatt
      481 ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac ggtactgggc gcaccaatta
      541 gctatcgttt gcatcctgat gcaaaaagat atacattgcg cgataatgga tttacagaaa
      601 caaacggagg caattatcaa ttgatccgac cattagatgc cacgccgcaa agcaaagaag
      661 gattcaaatt aaaaatcact gtatcaaaag atattcaaac attgaaaatg tcgatcacta
      721 cagcaaatgg attgaaagca gtcaatattt tcaaagatcc aaaacagaaa atgagccaag
      781 ataaatttta ttttctgatg gatgggatga ttagccgtgg gttattcgaa aaagtcgaat
      841 gatctgtgga tataaaaaga caactagttt tgaaacggcc tattgttaga tgactaactt
      901 tgtaaggacg acgcaaatta gttgtctttt ttatttttaa atttaacaca tgttgttatt
      961 tttaatattt tgctgattta ttgtttctaa gtaaagaaat ttaaattgaa acgattgtta
     1021 tttagggaaa aaggtatttt ttgaaaaaag aatcccgtat aatgattgtt gtgcaaacgc
     1081 tatacaaaat gaaaaagaaa agagggatgt ttatggaaaa tgaacagtcg gtcgttttaa
     1141 cgaattggaa gagaaattat ttgttctttt tatcgggaca gtttttgtca ggaatcacta
     1201 gtatggtcgt ccaatatgcg atcatctggt atttgacaag agaaaccggt tcggcgacaa
     1261 ttttgagttt tgctacttta ctaggaatga ttcccatggt tttactaagc ccatttgtag
     1321 gaccgctagt ggatcgctgg gataaaaaag ctcttttgat tgtcacggat attattgtag
     1381 cgattttcgc gcttatttta gcagtagtcg gaacgatttc agaatccttc ccgatttggc
     1441 tagtttttgt gtctttgttt atgcgttcgg tcgcacaaac attccaaatg ccaacgatcc
     1501 agtcgattat gccgacaatc gttccatctt ctcacataac aaggacgaat ggacagttag
     1561 gaatggtcca atcagcgaat ttcatcattg cgccagctct aggtgcggct ttgttttcag
     1621 tcgttcctgt caattacctt attttattag atgtattggg ggcagttttt ggtgtcggct
     1681 tattgatttt tgtgaagatc ccaaaagttt ctcctgaaat attggaagtt ccgcttacta
     1741 tttttaaaga tgcaaaattt gggctgcagc aattgatgga taataaaggg ttatggtaca
     1801 taacgatcaa tggtgcattt gtgatgctgt tgttcatgcc agcaatcagt ctgtatccat
     1861 tgatgacgct agattacttt ggtggttccg ttggacaggc gggagcagtg gaagtagtct
     1921 acgcagtcgg aatgctctta ggcggtgcac tgatcagttt tataggaaca tggaaagacc
     1981 gaatgaaacc tattattata gcctacatca ttatgggcct gacgatcggt gcaagcggat
     2041 tggttccaaa cgatagtcaa gggttcttgt actttcttat cttgaatgca ggagcaggtt
     2101 gtgcaacgcc atatttcaat acactgctga tggcaatgat ccagcagagt tatgaatcga
     2161 atgttttagg tcgtgtctta gggaacttca actctttgat gaacctggca ggtccaattg
     2221 gattattatt tgcaggacca ttagctgatc gtttaggggt agaaaaaatg ttcttgtttt
     2281 ccggaatcgg gattttatta tgcggaatcg ttttattttt aacgtcagct gcccgaaatt
     2341 atgataaaga attacagaaa aaactggtaa aagaacacca tgagcaaaaa gacgaatagt
     2401 atggaatgga gttgtgacag aagtcttgtt acaactccat ttctgatcct aatgttcctg
     2461 tggcaataag cctactcaaa tgaaaaatat gagtaattat ctttatttgt ttaatacttg
     2521 atatagggca gttttttcac gacctatcgt acttcctctt tgcttcatag atttctttga
     2581 tttgttgctc agataacgga gtgccaaatg gtagtttttc ctttaagagt tctctttccg
     2641 gtgcgtctac gcctacattg acatcgttct cgataggtac gctgtaatga tggatatgtc
     2701 catgaagatt gcggatagtg tcgttaggcc cgagcaacat cggataatga gtcaagtaat
     2761 attgttgatg gtcgaattta acgatggcac ctacatcata aaaataaaat ttgggacttc
     2821 cgtcagtaag cctaggatca tttttttcta aataacggaa aaaagcgcga ctgtcatggt
     2881 tccctttgat caagtggatt gttccttgca gttgagtgac cagttccaaa atttctgcat
     2941 ttgctttttc atattgcgga tgcatagcaa ggtcgcctag atgataaaca tggtcggttt
     3001 cttttattac tgcattccag ctagtgatca actgttggtt catttcatcc acatttgcaa
     3061 agagccgcgg agcaaagtcg ttttttccta ataagcgttc atggaaaaag tgcatatcac
     3121 tgataaagta attcatgtat cttttgcctc ccttttttct tagagtaaac aaaatcactt
     3181 atctgagcaa ctcataacat ctaaaaagaa aaggttgtaa atgagacgtg cagctttaag
     3241 cgataagaag caattttgaa aaatagtttt tcacaattta taaaattatt ccttattgcc
     3301 gaaaggctgg ccctttttat gagtatgcca attttttata tcgaatgctc aaaagatgag
     3361 tgattttttg gttgactatc atgtttgtgt actgaatagg tccagtagaa cagtattttt
     3421 acgacttgat aattaaggga tgaaattttc agaaatatga cgtataatgt aaaatactga
     3481 aaagttgcga ccttcaactc gtagatgttt cagaatctaa ttgaaatgat ggtgatatca
     3541 tgacagacaa cagcaaaaca cgcgttgtcg tagggatgag cggcggtgtc gactcatctg
     3601 taacagcact tttgttaaag gaacaaggat acgacgtgat cggaatattc atgaaaaatt
     3661 gggatgacac ggacgaaaac ggtgtatgca cggccacgga agattataag gatgtagcga
     3721 aagtagctgc tcaaatcggg attccttatt actctgtgaa ttttgaaaaa gaatattggg
     3781 atcgtgtatt tgaatatttc ctagccgaat accgcgcagg acgtacacct aacccagacg
     3841 tgatgtgcaa caaagaaatc aaattcaaag cattcttgga ttatgcaatg gatcttggcg
     3901 ccgattatgt agctactgga cattatgcgc aagtcactcg cgatgaaaac ggcgtggtcc
     3961 acatgcttcg tggagtagac aataataaag atcaaactta cttccttagc caattatcac
     4021 aagaacagct ttcaaaaacg atgttccctc taggcggcat ggaaaaatca gaagtacggg
     4081 caattgctga acgcgcaggc cttgcgactg ctaagaaaaa agattcaacg ggaatctgct
     4141 ttattggtga aaagaacttc aaacaatttt tgagcaacta tctgccagca aaaaaaggaa
     4201 atatggtcac tcttgatggt gaagtcaaag gacaacatgc tggcttgatg tattatacga
     4261 ttggtcaacg tcaaggacta gggatcggtg gcggaggaga ttcgcaagaa ccctggtttg
     4321 ttgtaggaaa agatctagcg acaaacacat tatatgtggg gcaagggttc catcatcctg
     4381 cattgtatgc cacaagcttg gatgcaagtg aaatccactt tacgacgaat gaaccaatgc
     4441 caaaagaatt caagtgtaca gcgaaattcc gttatcgtca gcaagacgtt cctgtgacag
     4501 ttcgcttatt ggatgataat cgtgcagaag tcgtattcga cgaacctgta cgtgcaatca
     4561 ctcctggaca agcagttgta ttttatgatg gaatggaatg tctgggtggt ggtttgatcg
     4621 accacgcata tcaagaaaca aaagttttac aatacgttta agcgatatta aatgaatcaa
     4681 aacagctatg gctaacaaaa gaaacacctt ttcttttgga tagcatagct gtttttttgc
     4741 aataaaatta atttttattc attttatact tgaaatactt gtgtaaaaag ttcataatat
     4801 ccaataatat gaataaagtt gctttttgtt aagcaagagc agttgcaaaa tagaagggaa
     4861 tcttttgcaa gtgatttgtt caattttata agtgagggtg atggaaatgg aaaagaaatc
     4921 gacgggtatt agcaaatggg gactggttgc tctggttgtc agttcgtcaa tcggaagcgg
     4981 tgtgtttggg atcacaagtg atctggctag ttctgcagct ccagggccag cgatcctttc
     5041 atggataatc gttgggattg gtattttagc tttggtatta tcgctgaatc atttaggaga
     5101 aaagcgtcca gatctagatg gtggtatttt cggttacgcg gaagcttcat ttggtaaatt
     5161 aggtggcttc atcagtggtt ggggttactg gttgtctgcg tggctgggaa atgttgcttt
     5221 tgcaacgatg ctgatgagcg ccatgggaga atttttcccg atattcaaag gtggacaaaa
     5281 tgttccttct attttatttg caagtatttt tatttggggt ctgactttat tagtaaacaa
     5341 tggagttgaa ggagcgagcg tcatcaatac aatcgtgacg atctgtaaat tagtgccatt
     5401 attcctattt ttaatatttg tatttatcgc atttaaagtg aatatattca ctgctgattt
     5461 ttggggtaac gtatctgata atctagtgaa tggaacaggg cagcaaggaa cgatctggtt
     5521 gcagattaaa ggttgtttga tggttatgat gtgggtcttt gtagggatcg aaggcgcaag
     5581 tgttttagcc aaccgcgcga agaaacgttc agaagcccag caagcatcga tcattggtct
     5641 gctttgttta ttgtttatct atatccttgc ttcgatcttg ccatacggcg tcttatctca
     5701 agaagagtta gcaactattt cacaacctgc tatggcaaat attttaaaag gaatcgtggg
     5761 aaattgggga gcagcgctga tcaatatcgg cttgattatt tcgatcattg gttcttggtt
     5821 gtcttggacg atgctcccag cagaaacaac gatgttgatg gctcgagacg gtctgttgcc
     5881 gaaaggttgg ggaaaaacga ataaaaaaaa tgccccaact tattcgttag ttgttactgc
     5941 aatcttgact aatctattct tattgacttt cttagttact gattatgcct atcaatttgc
     6001 ctattcttta tgtacagcgg caattttgat ttgttatcta cttgtcggga tctatcaagt
     6061 ccagttttct ttaaaagaag gaatcaaaaa acaagtagtg atcggtggga ttgctgtagt
     6121 ttttgaattg atgggcatca ttttagcagg attttcttat gtccttttat gtagtattgc
     6181 ctatcttcct ggtttttgct tctactggat cgcttgtcgt gaaaccaaac atcaaattac
     6241 gggaaaagaa aaagtaatga ttgggctgat ttgtgtggcc gcactgcttt caatcgggtt
     6301 gcttgctgta ggctggatca agatataagg gggaaagaaa catgaaaatc gcaaatttcg
     6361 gtgtagaaga atggctaaat aaatgggaaa agaaagcggt atatgatatt gcacaaagct
     6421 cgatcgaagc attgacatta gacgaactcg ttggcttaga tgggactagc gtaagtaaat
     6481 ttttcgaaaa acgcaaaaac gaaccattgg actatggctg gatcgaagga tcagatgttt
     6541 tcaaacaaga agtcgcttct ctttatcaaa ccgtcgatcc ggaaaacatc ttacaaacga
     6601 atggagcgac tggtgctaat cttttagctc tatacgcgtt agtggaagca ggagaccatg
     6661 ttgtttcgat gcttcctact tatcagcaat tatatgacat ccccaaatct ttaggtgccg
     6721 ctgttgactt tgttcatttg aaggaagaag aagattggca gtttgattta gaacagctag
     6781 aagccttagt caaacctgaa acaaaaatca tctgtttgaa tagtgcaaac aatcctacag
     6841 gcacattgct agatcggaaa actttagaaa aaatcgcaga aattgctcgt acagtagatg
     6901 catatgtatt gatagatgaa gtatatgcgc ctttaacgga taaaggtgaa ttcttgtcga
     6961 tcgtagatgt ctatgaaaaa ggtatagcaa ccaattccct atccaaaacc tactccattc
     7021 caggtattcg tattggctgg acagctactg gacctgagtt ggcagaagtt tttcgaaagt
     7081 atcgagacta cacgatgatt tgcggcggag tcctgtcaga tgatcttgct gtacatgcat
     7141 tgaagaataa agaaaaaata ttggcgcgaa acaaaaagat cattacagag aaccttgtga
     7201 ttttaaaaca atgggtagcc aatgaaccga aagttgaact tgtcgctccc aattacgtct
     7261 ctacttcttt cattaaatta gccatcgaag aagatgacca gacattttgt atcaatttat
     7321 tagaagaaac aggagtacta ttagtaccag gaagtgcctt tgatttaccg aagcatgccc
     7381 gactaggtta ctgttgtaaa aaagaaacct tggaaaaggg attattcctc ttatctgagt
     7441 tcttgaaaaa acaggcctaa acaaaaacac tgcaaggaca gtctgtgaca actgttcaag
     7501 cagtgttttt catttacgct tattgtctat gagaaggaga gtgccctgaa gctatggaga
     7561 ctcctgaagc tttttcttct gcagtttctt gctgatggat ctcttgtgta ttgtgtcgta
     7621 caagcaagaa gttgaccaaa acaagaagag aagtcgaaag aaagacgccg cgataaccaa
     7681 atgcactgga aacactggag ccgatcatag gccctactac gttaccagtt gcttggaaag
     7741 attggttata actgaagatc cgtccagcag cttgatgagg cgaatatttt gtgatcaatg
     7801 cttggacagc agggagcaga caagcatcag agataccaat caaaaaacgt aaggcagcta
     7861 attgccaaac attcgtgacg aatgccattg ggatataaac cagaatcgca aaacccaaac
     7921 caatcgcaag tattctttca ctcccaatct tgtcacctaa gcgaccaaat cttggagcgg
     7981 ctatcaaagt ggcaatccca ggtatcgaag caatgattcc gctgatcaac gtaacattcc
     8041 catgtccatg cagcaattgg cgaatgtaca gactgatgat tggactaatg gaattgttgg
     8101 atgcttggat gatcatcgtt gtgacgaaca taccgatgac gacgtgtgga tatttcaagt
     8161 ctttgaaaat ctgtttggca gaaaccatgt tttctttttt cactgggaca aatttttcat
     8221 ggacgaaaaa cagactcaac aagaagacga aaaataagat gatccctgta atgaaaaagg
     8281 tagggcgata gccaaacgct gaagcagaga tcccgccaag aagcggtcca agtaatgttc
     8341 ctgtaacaga accagttgct aaggttccaa gaacttgacc acttttttct tttggcgtcc
     8401 cagttgcgac aagggcagtg gcattactga tataaccaga aaaaatccct tgaagcagac
     8461 gcaatgcgac taattgatac acactagtca cgcaacccat caaagaaatg acgattgcca
     8521 ttcctaatga agcacgcaac agcatcaatt ttcttccttt ttggtcggct aattttcccc
     8581 accaaggaga gataagcgta gtcaccaaga aagtagatga aaaagtcaat ccgctccaaa
     8641 agttcaattg tgacgtacta taattaccta acgtatctat gtaaagtgac ataaaaggca
     8701 tgactaaact aaatccgata ccagccataa atgttccaaa ccataagaca aacaagtttt
     8761 gttcccaagt ttctcttttc ctaaataatt tttcctttat tcttgcagcc aattagactc
     8821 cttctttctg aaaattttca tctacagctc tttattataa actacaatat gtagtttatc
     8881 aatgaaagtc tcttttttct ctttcttttt cgtggtaaac tgaaagagtg aaggatttat
     8941 tttatgagag gaaacatgga aatgaaaaaa agaaatctgt ctcaagagaa aatcatcgac
     9001 tgctttcgag aactagcaga agagatggat gttcagcaaa taacctttca gcatttagcc
     9061 aaacgcttgg atatcaaggc tccctcgctg tataaccatt tcaaaaatat tcgtgatgtc
     9121 aaaacagcgc taacagcgaa acttctgcag gaacttaatg accagctgcg acgggctctt
     9181 gtgggaaaaa gtggtcagga agcactgcag atctatgcag aaacttatcg atcctttgct
     9241 ttttcaaatc aagcagtgta tgagttactg atcagtgtac cgcataccaa tgaagctgtt
     9301 ttattagatg gaatccatga aacgaatcag atcattttgc agatatttga ggcttttcaa
     9361 ttaacgaaag aagaaaaagt tcatcgaagc agggaacttc gtagtatgat ccacggttat
     9421 ctttccttac gttttttagg atactttaca aaagaaccaa caatcgatcc agaagacagc
     9481 tatttttgga tgatcaataa ctttatcgct actttgcctt aaacaatcag attcatttgt
     9541 aacaatttta agatgttcat taaatgaccc ttttataaga gaaaattctt gcaactttct
     9601 tgaaaagtga ctaagatgag tctagaaaag ctgaaagaag gagcacgagt atgtatcaag
     9661 taattaccat gttcggggac aatgagcctt ggtggttttt tgaagactgg gaagaagata
     9721 ttgaagaaga agaaacattc gagtctttcg aagaagcacg acaagcgtac gaaaaacaat
     9781 ggaacgaaat ccatcaaaat tatgaatata tcaatgcaaa aagtaacttt ctcagcgcct
     9841 tttggaatga cggagatgag cgctggtgtg aagagtgtga cgatgattta cagcaataca
     9901 agggacttgc gttgttgaaa aatcatcagc ccatcacagt agaaagcaga aaggaatttt
     9961 atgaaacaac taattctagc ggaaaaacca agcgttgcga aagacctaag caaggtgctt
    10021 ggtgctaatc aaaagcacaa aaattattat gaaggaccca aggtgatcgt tacttgggcc
    10081 cttggtcatt tattaggatt gaaaatgccg gaagatctga ataaggaatg gcagagctgg
    10141 caaatggaca cgttgccaat gatccctaaa aaactaggaa tcaaaccact tcctaaaaca
    10201 ggacatcaat taaaagcaat caagcagctg gctcagcgaa aagatgtctc agaagcagtc
    10261 attgcgacag atgccggaag agaaggggaa cttgttgctc gctggatctt agagtatgtg
    10321 catttcaata aaccagtgaa gcggttatgg atctcttctc aaacagataa agcaatcaaa
    10381 actggattca agcaactaag acctgcgaaa gaatatgatg cattgtatga atctgctctt
    10441 gcccgggcaa aagcggactg gctggtagga ttgaatgtga ccagagcctt aactgtgaag
    10501 tacgcggata atttatcagc aggccgtgtg cagacaccga cactagcatt agtgagagac
    10561 caagaaaaga aaatcgagca gttccgtcca caaacgtatt tcacgatcac gttgcaagct
    10621 ggaaaagaac aagccaaaat gcttcaaaaa aatcaatttg ctttaaaaac acaagaagaa
    10681 gcacaaaaac ttgttcaatt gatcagtaca agcaaaggaa ctgtcacaga agtccaagaa
    10741 aaattaaaaa cggaatctgc gccattgcct tatgatttaa cagagatcca aagagaagcc
    10801 aatcaacgct atggctattc tgcaaagaaa acattaagtc ttgtacaaag tttgtatgag
    10861 acccataaga tcgttactta tccgcgtaca gacagcaaat atctgacaaa tgacatgaaa
    10921 agtacgatga aagaacgatt gcaagcagtc agcgactttg ctcctgaggt caagcagtat
    10981 ttgaaaaacg gagccatcgt ccaacagaca aaagtattcc aagacagtaa agtgaccgat
    11041 caccatgcac ttttgccaac tgaaaacaga gcacgttatg aaaaattaag taatgaagag
    11101 cgaaaaatct atcaaatgat tgtctctcgt tttttaggac tgtttgctca accccataaa
    11161 gtcagccaaa caaaagttac ggttgaattt ggtaaagaac gattcatttt tcggcaaaat
    11221 cgtgtcgtcc aagcaggatg gaaaggcgaa actgaaactg agacggaaac agtcaaatgg
    11281 gaaaaaggaa tgagaatcaa tcccgatttc gccatcaaaa aagaactatc tgcaccgcct
    11341 aaaccattgt cagaagcaag cttgcttggt caaatggaaa aatacagtct gggaacccca
    11401 gctacgcgtg cagagattat cgagaaacta atcaaatctg aactgatgga acgtacccct
    11461 caaggactgc aagtatctcc taaaggtaag caattattag tattggtaaa tccctcttta
    11521 gtaacaccag atttgacaga aagctgggaa aaagaccttg aagctattgc tgcaggaaag
    11581 aaacaagcag gaaccttctt gaatggaatc gaaaaagaaa caaaacgctt ggtgaatgag
    11641 atcaaatcaa gcaaacaaga atatcaagat ttctcgatta cccaaaagaa atgcccagaa
    11701 tgtggggcca atctgcgcga gaaaaacaca cgtgacggca aaatctatgt ctgcaccaat
    11761 tcagattgca actaccgcag aagaaaagat ccaaaagtct ctaatcatcg atgcccgcaa
    11821 tgtcaccgga aaatggaaat catcgaaggt aaaaacggtg cttatttccg ttgtaaatac
    11881 gatggaacga cagaaaaatt gcttgataaa aaagagcaga agaagaaaat gaccaaacat
    11941 gaagaacgac gcttgctgaa aaaatatgca caagcagaag aaccagaaga aagcccatta
    12001 gcagcagcac taaaagcagc aatgaagaag taataagata tgaaagaggt tgtga
//
LOCUS       DASJON010000083        11950 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJON010000083 DASJON010000000
VERSION     DASJON010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.083"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..862)
                     /locus_tag="U3989_001637"
     CDS             complement(5..862)
                     /locus_tag="U3989_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1715089.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            982..1893
                     /locus_tag="U3989_001638"
     CDS             982..1893
                     /locus_tag="U3989_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1715090.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            1900..3546
                     /gene="mdcA"
                     /locus_tag="U3989_001639"
     CDS             1900..3546
                     /gene="mdcA"
                     /locus_tag="U3989_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN1715091.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            3648..3947
                     /locus_tag="U3989_001640"
     CDS             3648..3947
                     /locus_tag="U3989_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN1715092.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            3937..5613
                     /gene="mdcD"
                     /locus_tag="U3989_001641"
     CDS             3937..5613
                     /gene="mdcD"
                     /locus_tag="U3989_001641"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN1715093.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            5617..6210
                     /locus_tag="U3989_001642"
     CDS             5617..6210
                     /locus_tag="U3989_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN1715094.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            6234..7310
                     /locus_tag="U3989_001643"
     CDS             6234..7310
                     /locus_tag="U3989_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN1715095.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            7335..8204
                     /gene="mdcB"
                     /locus_tag="U3989_001644"
     CDS             7335..8204
                     /gene="mdcB"
                     /locus_tag="U3989_001644"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN1715096.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            8315..9079
                     /locus_tag="U3989_001645"
     CDS             8315..9079
                     /locus_tag="U3989_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN1715097.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            9568..11661
                     /locus_tag="U3989_001646"
     CDS             9568..11661
                     /locus_tag="U3989_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1715098.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            11686..>11950
                     /locus_tag="U3989_001647"
     CDS             11686..>11950
                     /locus_tag="U3989_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715099.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEE"
BASE COUNT         3919 a         2107 c         2559 g         3365 t
ORIGIN      
        1 ttttttattt tcctaaataa aattcccgca tcgtttttgc aatcattttg atcaaaggat
       61 gatcttctgt gaagctagaa gataaataaa ttgttcgtgt caagggcaaa tctttgattt
      121 cccgcaagac gaaaggatca tttttatcgt aagaccaaga aacttttggg aaaaagctaa
      181 cagctaatcc ttgcttgaca atagaacgta atgtcgcagg atcatctgtc gtcgaactga
      241 tcgtcaattc gatacttttc gcttcaagtg ctgcatccag tgtatgacgg aaggcgttgc
      301 tctttgacag catcaagatg ggggtgcgtt ggatatcttc taattgcaga tgttcttttt
      361 tagcaagagg atgtttctta ggaatggcga cataaacagg ttctttcatg ataggaatat
      421 tggtcatgcc aggcttcatc tccgaagtga tgtacagtac aggatcggta atatcatctg
      481 gtaaatgatg ttgcatgatc gttaattgga tatcaggatg cttttccata aaaattcgga
      541 ccaagttcgg aatagaaaca gaggctacct ctaaatacaa tatgacggaa gtaacgatct
      601 tctcattgac cgaagacaat aatttgattc cttcttctaa tgtgtctaat gtcttgactg
      661 catatggata aaattcttct gcataaacgg tcgtctcaat gtttctgccg ctatggtaaa
      721 acaaagggac acctaattcg ttttccaaat gtttgatcgc acggctcaag gcaggttgag
      781 aaataaataa ttctttggct gcttgggtaa cattttttgt ttcgaatact tttttaaaat
      841 actgcagatg aagcaatttc ataattatcc atcccatcca tacctttttg ttatggtttt
      901 ttcttttttt ggcatttttt attatagcag aaattgacta agatagcagt ggaaaaagta
      961 agtgctgagg aggcgaggct catgagtaca ggtttcttat ttcctggaca aggcagtcag
     1021 aagttaggga tgttagcgga tatccctgaa atgtacatgg aacgtttaga aaatgtgacg
     1081 gggtatcggt tagttgataa agaagaaaac tatcaagata cggtttttat ccagctagca
     1141 ttactgacaa aagcagtatt ttatttagat gaaatggaaa aaaacggtat tcagcctgat
     1201 attgcagcag gacattcgat tggtgctttt tgtgcagctg ttgcttgtcg atctttgaga
     1261 tttgagaatg cagcggaact agtctatcac cgcgcagtct taatgaaaga agcttatcca
     1321 agcggttatg caatgggcgt agtagtaggt gtgacgagaa gcgaagctga ggaaattgta
     1381 gcaaaaacat ttgatcctga ccatccagtt tatctgtcaa atgaaaattg tccgatgcaa
     1441 catacgttat ctgggagtat cgaaggttta tccgttacgt tggcagaagc caaacggcac
     1501 caagcgcaaa cagcgaagct gctgaaagtc cctgtgccat ctcactgtct cttgatgaat
     1561 gatacggtca agaaattcaa gccgtatatg cagtctgtcc atctatctga tgctagatgt
     1621 ttgtatataa aaaatacgga tggaagagcc actcaggacg cagaagagat tcgtaaagat
     1681 ttgttggaaa acctagcttt ccctgtgcag tggaaccaaa tgatggactt ggcaaaagaa
     1741 ttaggaatgg atctgacgat tgaatttccc cccggcaata ctttgacaaa gctgattcat
     1801 acaaaatttg gtgtagataa agggatccgt acgatcaatc tagaccaaca tggtattgat
     1861 gacgccacgt ttctatatca aaaatggaga tgactaatta tggaaaaacc aaaccgcaac
     1921 tgggcttcta aacgcgaaaa aaaagcgcag cgattggaaa aaatcagcaa cttgctcaat
     1981 ggcaagtatg tagaaactga aaaaatcgta gaagtaatgg aaaaactgat tgctcccggc
     2041 gacaaagttg tattagaagg cgacaatcaa aaacaagcaa gttttctttc agaaagtctt
     2101 gaaaaagtag atgcagaaaa agtgaatgat ctgcatttga tcatgtctag tatctcacga
     2161 ccagaacatt tgaatatttt tgaaaaagga atcgcaagca agatcgattt ctcttatgct
     2221 ggtgcacaaa gcttgcgtgt cgcccaaatg attgaagacg gcacaatgaa attaggtgat
     2281 atccatactt atttggaact ttatggacgc ttattcatcg atttgattcc aaatgttgtt
     2341 ttagtagctg cagataaagc agataaagac ggcaacttgt acacaggttt caatacggaa
     2401 gaaacaccaa caatcatcga agctgcagct ttcaaagatg gtatcgtgat cgttcaagtt
     2461 aacgaagttg tcgattcttt accacgtgta gatatccctg gtgactgggt agacgttgtc
     2521 gtgaaagcag ataaacctta tcaattagaa gctttattca cacgagatcc tcaaaacatc
     2581 acagaattgc aaattctaat ggcaatgatg gcaatcaaag gaatctacgc agaacacggc
     2641 gtacaatctt tgaatcacgg tattggattc aatacagcag ctatcgaatt gttactgcca
     2701 acttatggtg aatcactagg gttaaaaggg aaaatcgcga agaactgggt attgaaccca
     2761 catccaacga tgattccagc catcgaatca ggttgggttg aaagcatcca tagttttggt
     2821 ggcgaagttg gtatggaaaa atatattgct gctcgcccag atgtcttttt tgtcggtaaa
     2881 gacggcacga tgcgcagtaa ccgaacatta tcacaagctg caggacaata tgccatcgat
     2941 atgtttatcg gttcaacctt gcaattagat tacgaaggaa actcttctac cgtaacaaaa
     3001 ggacgtttat ctggttttgg cggtgcgcct aacatgggac ataatcctgg cggtcgtcgt
     3061 cattctacac cagcatggca atcacttcgc gacaatgatg atccattagg gaaaggccaa
     3121 aaattagttg ttcaaatggt tgaaacatat ggttcaaata aaaaaccagt ctttgtcgaa
     3181 gaattagatg cactagctgt tcaaaaacaa gcaggcttag ccaatgcacc agtgatgatt
     3241 tatagcgaag atactacaca tatcgtgaca gaagaaggaa ttgcttattt gtacaaagca
     3301 aaaacaaaag aagaacgaca agcagcaatt gcagcaattg gcggtgttac acctcttgga
     3361 atgactagca cgaaagaatc actagatagc ttgcgtgcag ccggcatcgt gaaattacca
     3421 gaagatctag gcatcaaacg tagtgaagcg aaacgttcat tattagctgc acaaaccatc
     3481 gatgatttga tcgaatggtc agatggcctg tatgaaccac caatgaaatt cagaacttgg
     3541 tcataaaatt ggcgagaaaa accttttgtc gccatttacc cagatggaag aagaaagacg
     3601 aataagcaga acgtgtatgc tctgttttac aaaaagggga gagatccatg gaacaattag
     3661 cttataaata tccaacgaaa cagccgatca ataagaaaat ccatgtagga gtggtcggtt
     3721 caggagattt ggaaatcttg atgtttccaa cagaaaaaac agaatctact gtaaaaattt
     3781 gtacaggaag cgatggcttc ggcgaagtat ggaacaatgt attaacgaga ttttttgatc
     3841 gttatccaat ttcagcagat atcgtgatca atgacttcgg tgctacacca ggagttgtgt
     3901 acctacgatt gacgcaagca atggaggaac tttcagatga aaaatagttt tgtagaatta
     3961 aacgccagag aacgagtgaa cgctctactt gacaatggac gtgaattgat tgatccattc
     4021 gatcaaatca tcgcgccaag tttaccagca caaggaatcg tccctgaaag tgatgacggg
     4081 atcgttgtca gccgtggcac gatcaatggc aaacaagcag tagtaatcgc tatggaagga
     4141 aaattccaag gtggcggtat cggtgaagtt tccggtgcaa aaatcgttgc tgcacttgaa
     4201 catgtgttag aagaaaataa aaaaggaaat gagatctttc cagtcattgt actagataca
     4261 ggtggtgttc gtttgcaaga agcaaactac ggactgttat ctatttcaga aatcggaaat
     4321 gtaatcattg ctctaaaaca atatgtacca gtcatcggtt tgattccagg acgtgtagga
     4381 tcatttggcg gtatgtcgat tacttcagca ttaatgagtt atttaatcgc aacaaaaaaa
     4441 gcgagagtcg gtttgaatgg accagaagtt attgaacaag aagcaggcgt tcgagaattt
     4501 gattctagtg acaaagactt gatttggaat acattaggat ctcgtcaacg ggtagaatca
     4561 aaaatcattg atgaattagt gactgatagt gtagaagcaa tcaaagctgc tgtcatcgca
     4621 gcaatggacg aacgaaaaga cacgcatcgc agtgaaagct cagcatttta tttatcatta
     4681 cttgattctt tagatctttc aaaaccaatg gccattgacg attacaatga agcaattgct
     4741 aaccatcaag caaaatcaat tgacttacca gaagtagcag aaggaaatga accagctgca
     4801 gcaagtgttg gttacgattg gtttgcggca ctaactggca tgaaaaatcc aacttcttct
     4861 attagtactg tgtattatgg taaatcaagt aaattcatag aagaagccat cgtgacatca
     4921 atcgtacctg atcctaaaaa cccgatgtac cgtgtacgaa acggtgaagt tggtttagtt
     4981 gaaggtttcc gcatgggaca aattttggat cacgtttacg aagaagataa agacaaaaaa
     5041 gtgaaacggc caatcatcgt tgtgatcgat gtaccaagcc aagcatacgg ctataacgaa
     5101 gaattgatcg gtatccatgt ggctttggct aatagtgctg ctgcttatgc aaaactccgt
     5161 caagctggac atccagttat tggattgatt gcaggaaatg ctatttcagg tgcattttta
     5221 gcacatggat tacaatcaag ccgcttgatt gctttaaaca ataaagaaat taccgtccaa
     5281 gcgatgtcta aagaaagtgc tgcacgtgtg acaaaacgtt cggttgctga aatcgaagca
     5341 gctgctgaaa aagttccagc tatcgcttac gatatcaata attatacaaa actaggagcg
     5401 atctatcatt tcctagaaga tatcaccgac caagctgctt cagaacaaaa tgtcagcaaa
     5461 gtcatcgaag caatcaatga agccattcaa gatgtccgca atacaaatga cacgatgctg
     5521 acaaatcgat acacaaacga aacagccaaa acattcggac gtgtcgaaac aaataaagtc
     5581 cgtgccaaaa tggacgaaga atgggatgca taaaaaatga ttcaagcaca tgatattgtc
     5641 ctttttgata aacatgaaat agaagggttc accttgcctg aatgggtcgt tctaactgaa
     5701 aagatgttcg gaacagttcg tcgtgcgtcg acggatcacg cagaaatact accgattggt
     5761 ttacgtggac ctgcccgcaa tcagagatgg tcaacagaaa tctcaaaaca tgccgtcaaa
     5821 caagtcattc atccgtggga catcatcgaa caagaatcgt atagacaaga gaaaatcatg
     5881 gattatcctg tttatcagca atttcatgct gctagaaaca tattatcagg atgtaaatgg
     5941 ggaatcggcg gcagcttagg atttgaatta tcgactggga ttccggcagt aaaagaaacc
     6001 agtgattttg atttgctgct gtacgcaaat tcaccgattg aattgccaat tcaggtaatt
     6061 caatcgcatc ctgctttttt tgagcaattc gatacccaag tagtgactcg gcaaggcggt
     6121 ttttcactaa aagagtattt gagagagcca gagaaaaaaa ttctattgaa aacagtcgaa
     6181 ggacctaaat taaccaaaga actttggtaa taaagaaaaa gagagagaaa gagatgtttg
     6241 aaaacaaaaa aatcaaaatc aacgtaagtg acttccagaa agtatcagct gtaacggctg
     6301 ttatgatgca aacgatattg agctttgctt tggcaaacag ccacagcgca acaaatacag
     6361 cttttcttgg taccttttac gtcatcgtta aatacacagc accaatgttt atttttgcaa
     6421 ttgtttataa tatggtaaaa acaagtcagc atttgtctta ttgggaattt ttgaaagaaa
     6481 agttttttga actagtcgtt ccgtatcttc tatggacgac ggcttatctt tggctatttc
     6541 cagcagtcca acaacaaact ccgtatacgg atactgcaag ctttttactg aaatatgtca
     6601 caggtgatgg agcgcctcat ttatggtata ccgttatgat gctgcagatc caattgttca
     6661 tgccgttttt tgtttggctc ggttacaaag tatttgctaa taaaaaatat gtatggccag
     6721 tattgattgg ttcaacgatt ttatatgtcg catggtatct gttctatcaa acacaagtat
     6781 tgactgggcc ttatcatgac agctggtact tattagatcg gtttgttgct agtttcatga
     6841 tttatggggt agccgcattt gtttatcacg aaaaagtcta tcaatatttg gacagagtac
     6901 gttatctttt cttacctgtt gccttagtta tcgcgttctt ttctgtccgt tcattgctcg
     6961 cacatccagg agacttgagc tttgcgaatg caccttactt gaatacgatc caaagtttat
     7021 acagtttagt catcatcttt gctgtcttca taggcgcttc caaaatgatt gtaaacgatt
     7081 cgccaaaatt gccattgttc aaatggttgt ctgtttatgc ttatcgtacg tatttagcca
     7141 atgtatttgt tttccaagta ctgttattat tgtttaaaga tagttggctg cagttacctt
     7201 caggagtcat gattcttgta gcttatctaa tgacggcaag ttgtgcattt gctttaagct
     7261 ggttacttca tatcatttgg gtagctatca aaaaaggttt cagcaagtaa tagtaggaag
     7321 aaggagtgag tgtcatgata aatgacgaac gaaaaaaagc agtgactcag caactcgctg
     7381 cgttggtaga ggaagcattg atcgcagaag taacattaag tccaaaacct ggattagtgg
     7441 atgcactgga tgcgggtgca cattccgata tggactatgc tttatttctt aagtctgcta
     7501 agacgctaac tccttttttt gaagaaatgg ctcaaatagc atggcatcat gctattgatc
     7561 aggagttaag agaaagaatt gctgagattg ggcgaaaagc ggaaaaggca atgctgattg
     7621 ctacaaatgg catcaatacc cataaaggag cgatttgggc aatggggcta ttgatcagtg
     7681 tcattgcaca aaaaatcagt atggaacagc ctttttcttt atcgaaaata cttgatgatg
     7741 tcagcaagct tgctgctttt cctgatacaa aatatcgtca taaaaaaacc actcatggtc
     7801 atagggtgaa gcaaaaatat ggcgtaaatg gtgcatatga agaagcgata ttagggtatc
     7861 cgcatatcag gcttgcaatt caagcagcta aggagctaga taatgattca tatgtacaga
     7921 aacaaatgca tatgttgtta gttttgatga gttcgctaga tgacacatgc gtattgcatc
     7981 gcagtgatca gaaaacgtta gtggaaatgc aaaaacttgc tcatgctgct agtcaaagtg
     8041 ctttaccaaa tcgtccattt acagcactgt tgcacttttg tcaggaaaac caaatctctc
     8101 ctggaggaag cgcagatctc ttgtctgcaa gcttgttcat gctgaagata gaggaattat
     8161 ggactgccga cacatacatt tgtccgatgt tactcaatga ctaaataaga caaaattgct
     8221 aaaaaaacag tttatgcatc tggttattga ctcttccttt tagcaaggct atcataaaaa
     8281 tagttttttg taataaaaga taacgaggaa gacaatgaaa aaaatcaatt atttagcaat
     8341 cattttgtta gtgggcctat gtttgtttgc tctgccgacc caaggattcg ctgtcgcggg
     8401 tggtcatggt ggaggtacat ctagcggtcg aagttcgggc agaggttcat ttggtggtcg
     8461 ttcatatggg ggcagttatt ttggcagtcg ttcttacggc agagattctt atgggtatta
     8521 tggtccaggt acaggtcctt ctagttatga gcaaacggga tttcttgcat tagctgtttt
     8581 gtcagtagga ggggtttctg ctttttctat cgtcaagaat aaaagacaaa agaaaaggct
     8641 gaaagcggag atattgtccc gcatgtcagg aacggctaaa gaaaagaaag cacagtttct
     8701 ggaaatccaa aagatattca tgaagatcca atccgcttgg gaagaagaaa ctcctgaaaa
     8761 agccagaagc tgttatacag acaagctgtt tgccgaacat cagaaagttc tgcaaaaaaa
     8821 caaagcagac ggtgtaagaa atcatactaa aaaaataact attcaggaag taggaaatta
     8881 tcgacagatc cacgaaaata gttttgccat ccgaattgat ttcagatgct gtgattattc
     8941 agtcgatcga aaagatgggc gattgatctc aggaagtaag acacaacgac agtatttttc
     9001 tcagatatgg tattttgatt tttctgagaa agaaaaaaga tggcaagccg attttattca
     9061 gccgattatg ctggattgac aggaaatatt tatgaacgga gttgtgacaa aagccttgtc
     9121 gcagctcctt tttctgaata aacggtgtgc cagagatagc ctatcggcaa taaggcacga
     9181 ttttgtaaaa tatgggaagc gatttttcaa aatcgcttct tatcgctcaa ggctgaacat
     9241 ctctgtcaca acctctccac taaatggtgt ttaaaagctt gcatctgcga taataagaac
     9301 aaccaaacga aaaataggaa gcactatttt agtttagttg ttcttattac ttgatgcagg
     9361 acagcttttt cacaacctct tttttgtata gatgggcaaa atttttgtaa taataggcgt
     9421 atgtcttttt cgttgaggag atgaaaaagc atatggctta ttttttgttt gctttgctta
     9481 ctttctttcc acttcaagtg gcagaaatat ctacttttta ttatgtaagc ggaaccatat
     9541 acttgaaatc agaaggagat catttctatg aaaacaagaa caattgaaat acttcacaga
     9601 atcatgacag cccaatatcc agtaaaagca aaacatctag caagtgagtt caatgtcagt
     9661 cagcggacga tccgtcagga agttcttgaa gcgaatatct ggttgagcag taaaaagctt
     9721 ccggagatcc gtacaattcg caacaaaggt tttttgttga agctggaaaa ggaaaagcaa
     9781 gctcgattag aaactgaaat aaaagatgtg aaagacggct ttttgaatcg gcgcgaacgt
     9841 atttttgatc tagttttagc cgttgcttac agccgaatcc cgatccatct aaacaaaaaa
     9901 gaagaagaat ttcaaatctc taaaagtaca ttagatgaag atatgagacg gttacgagca
     9961 acactggtca gttatgggat agagatcgtc agttatggaa agcaaggact ggtttataaa
    10021 ggttcagaac gatctatccg tacgatgatc tatgatttta tcaatcagaa tctgggaatg
    10081 accgacttct ttatgaatca agatgcacaa ttaacaccgg ctcaaaaaat ctttctccat
    10141 tattttcctt tggtggaaat caaaaaaata caggagatct attccaataa gttgttgaaa
    10201 caagaagatg atatttataa aaaacaaatc ctgcttttta cattgatctg gatccagcga
    10261 atcaggaaac atgaatttat ctctgccatt aattggaaaa atacagaaat aaaagacagt
    10321 agcttttctg catttattga ccacatgatc gaggaatttc aattggacaa tgtcccgcaa
    10381 gtagaaagaa attatcttcg tttcactatt gagacgttca atgctagaga tatctccaat
    10441 tcaatcgagt gggtacaagc tcaattacta acgatccaat tgatccaatt tgtagagaac
    10501 gagacacacg tacctttcca tctaaaggaa gaagctttat tcgaaaatct ctataagcac
    10561 atggctgcac tgattgtaag aatcaagaat catatccaag tcatgaatcc attgaaagaa
    10621 aacatccgaa caaattattt cccaatctac cgcgcagtca ctcattttgt tcctacgatc
    10681 gaagaagtaa ttggtggcaa gatactggag gacgagatag cttttcttgt catccacttt
    10741 tcgacgatcg ccagtgtaat caagcgagac ttgaattatg tttataaatc agtcgttgtc
    10801 tgtaaccatg ggatggctac tggaaatctg ttagcagaga atctgaaaga aaagtttcct
    10861 cagatcgaga ttactgcagt tttaagttca aaagaaatcg aattagtaga taaattggat
    10921 gtcaatctgg ttttcagcac ctttcaattg aattatcgaa ataagccgct tttagttgtc
    10981 gatccgattt taactgagac gaatatcttg gttatagaag actttttgaa aagaaatgaa
    11041 gaactacagc ggttagagcc taacagtcat gaatcgacgg agttattcac tcaaataata
    11101 gatgtcatga aagaaagtgg tggccgagta gatcgaatca tctatggcaa gttagaaaaa
    11161 ttattcgaaa caaacaattt agaaatcaac aaaagggaga tccagcctat gttaaaagat
    11221 attttgacgg acagcaatat catgatcaaa gaaaaagcca ctacttggga ggaatcgatt
    11281 gaactagctg ctagaccatt ggttaaagaa aacattgtcg aagagcgcta tgtcgaagcg
    11341 atgattcgtg cagtgaatga ttacggaccg tatatcgtga ttggcaaaca catggcacta
    11401 gcccatgccc gaccagaaga tggggtcaat aaattaggag tcagcgtagc tacgattgag
    11461 caaccaatcg atttcggtaa tcctgaaatg gatccagtaa agattatttt ttgtttggca
    11521 gcagtagact cctactcgca tctgaacatc atgaaagaac taattgaatt gatcaacaac
    11581 gaagagaagt tggatcgatt gattgaatgc acaaatatcg aagatttcaa acaactgctt
    11641 ttttcagtag ttgagaaata ataaatgata cgggggagaa caaagatgaa caaattgaac
    11701 attttattcg tatgcggtgc aggtttagga agtagttttg cagcacagat gtctgctgag
    11761 gatgttttga acaaattagg agtagaagcc aaactggacc attgtgatat ttcttccgca
    11821 gtttccatga aaccagatgt gatcatcaca gcagaaaatt ttcgttcgca gttcgaaaag
    11881 ttcgcagttg atcctaaaac ggcgatgatc tatttgaaaa atattgtttc aaaaaatgaa
    11941 attgaagaaa
//
LOCUS       DASJON010000084        11887 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJON010000084 DASJON010000000
VERSION     DASJON010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.084"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..581
                     /locus_tag="U3989_001648"
     CDS             <1..581
                     /locus_tag="U3989_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1715100.1"
                     /translation="FLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEVYDL
                     PDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVWQNE
                     QTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGYGPK
                     GKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            712..2046
                     /locus_tag="U3989_001649"
     CDS             712..2046
                     /locus_tag="U3989_001649"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN1715101.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            2353..2838
                     /locus_tag="U3989_001650"
     CDS             2353..2838
                     /locus_tag="U3989_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN1715102.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            2831..3514
                     /locus_tag="U3989_001651"
     CDS             2831..3514
                     /locus_tag="U3989_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN1715103.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            3640..6285
                     /gene="polA"
                     /locus_tag="U3989_001652"
     CDS             3640..6285
                     /gene="polA"
                     /locus_tag="U3989_001652"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN1715104.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            6330..7166
                     /gene="mutM"
                     /locus_tag="U3989_001653"
     CDS             6330..7166
                     /gene="mutM"
                     /locus_tag="U3989_001653"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN1715105.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            7130..7759
                     /locus_tag="U3989_001654"
     CDS             7130..7759
                     /locus_tag="U3989_001654"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN1715106.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            7811..8143
                     /locus_tag="U3989_001655"
     CDS             7811..8143
                     /locus_tag="U3989_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1715107.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            8455..8946
                     /gene="nrdR"
                     /locus_tag="U3989_001656"
     CDS             8455..8946
                     /gene="nrdR"
                     /locus_tag="U3989_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN1715108.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            8969..10342
                     /locus_tag="U3989_001657"
     CDS             8969..10342
                     /locus_tag="U3989_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1715109.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            10342..11268
                     /gene="dnaI"
                     /locus_tag="U3989_001658"
     CDS             10342..11268
                     /gene="dnaI"
                     /locus_tag="U3989_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN1715110.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            11397..11837
                     /locus_tag="U3989_001659"
     CDS             11397..11837
                     /locus_tag="U3989_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1715111.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         4074 a         2078 c         2597 g         3138 t
ORIGIN      
        1 tgtttttggt cggaggggtg gggcatgcga ctagaatcct ttatgagaat tttgaaaagc
       61 aaggatttca ttttgaagat ggcatgagtg aaagtgaaat ctgtcgtcag tatctaaaag
      121 aagtctatga tttgcccgat aaggcttttc tgatagaatc gaagtctaca aattctggag
      181 agaacgccac tttttcactg gagattcttc attccttagg agctgtacca gaaaaaatct
      241 tattgatgaa tgatccgaca ctgcaacgca ggactcgagc aacttttgag aaagtatggc
      301 aaaatgaaca aactattttc gcgaatgctg tgccatttgt gccggaaatc cttcactttt
      361 ctgaagagat catctttaca gcaaaagaac tgaatcatca atggcctaag gaatatttcc
      421 atgcgctagt attaggagaa atggaacgtc tccatgacga tgagaacggc tatggtccga
      481 aaggaaaaga ttttattcct cacattgata tatcagagga agtttggtct tcttacacca
      541 gaattaaaga gtcaataaaa acggattttt ctcgtacttg accattttag caagcgaatc
      601 ctcctttcca aataaatttg gttgaaattt tcaatcgttt ataagataat gaggagcgtg
      661 ttgaatgtaa tagctaaaag aatggacgct agataagaga agaggattca aatggaaaat
      721 caaaataaat tgtatcattt tgttggtatc aaaggttcag gaatgagctc cttggctctt
      781 gttttacatg aacaaggtct aaatgtccaa ggatcagaca ttgaaaaata tttctttaca
      841 caaagagatt tagaaaaagc aaatatcacg attctgccat ttaacgcaga taatgtaaaa
      901 ccagggatga cgatcattgc aggaaatgca tttcctgatt cacatgaaga aatccagcga
      961 gcaaaagaat taggtttgga agtgattcgc tatcacgact ttattggtca ttttatccaa
     1021 aattatacga gtatcgctgt aacaggctct cacggaaaaa caagtacgac tggactgctc
     1081 tctcatgtat tgagtggtgt ccgtccaaca agttatctaa ttggagatgg aacaggacat
     1141 ggtgatccgc aagcggaatt cttttcattt gaggcctgtg aatatcgccg tcatttcctt
     1201 gcctattcac cagactatgc aatcatgacc aatatcgatt ttgatcatcc ggattattat
     1261 acaagtatcg acgatgtatt tacagctttc caaacaatgg ctggacaagt gaaaaaagct
     1321 atttttgcgt atggtgacga tgcttattta cgcaaattaa aagcaaatgt gccgatttat
     1381 tattatggtg tcacagaaaa cgatgatatc caagcacgaa acatcgaacg cacaacttct
     1441 ggttctgctt ttgatgtcta tcatggggat gaatttgttg ggcacttcac tgtaccagca
     1501 ttcggtaaac acaatatctt aaatgcttta ggtgttattg cagtagctta tttcgaaaaa
     1561 ctggatctga aagaagttgc agaagaaatg ctgactttcc caggagtaaa acgtcgattc
     1621 agtgaaaaga tcgtagcaga catgaccgta gtcgatgatt atgcgcatca ccctgcagag
     1681 atcaaagcaa cgattgatgg cgcacgccaa aaatatccag ataaagaaat aattgcagtt
     1741 ttccaacccc atacgtttac gcgtacgatt gcattgatgg atgaatttgc agaagcactt
     1801 gatttagctg ataaagttta cttatgcgat atttttggtt cggctcggga agaacaagga
     1861 aacgttaaga ttgaagacct tggagcgaaa atcaaaaaag gtggagaagt catcaaagaa
     1921 aataatgtat ctccattgtt agattatcat gatgcagtag taatcttcat gggtgctgga
     1981 gatgttcaaa aatttgaaca agcgtatgaa aaacttttaa gcagcacgac gaaaaatgta
     2041 ctgtaataca attactgcag tgtccttata aaaatagaaa gagggggagt ggcaaaaatc
     2101 ttgccaagcc cccttttttg caacttatga taaacggtgt tcaaaagctt gcatctgcgg
     2161 taataagccc aatcaaatca aaaacatgag aagctgtttt ttcttggctg ttcttattat
     2221 ttgatgcagg acagcttttt cacgacctct gtaaactata ggaatctgtt aaatcaaggg
     2281 agcagaactt gccttttacg ctacttttgg tacaataatg tatgattaaa aataaactgg
     2341 agggtttcta tcttgaacat aggtaagccg agaaaactag gaaaaacaat aaatgaaatc
     2401 gaggaaggcg actcgttatc cttaacagaa tctattgaag ataaccagct tttgctgtat
     2461 ttgggattga cgaatgatgc aaatcctttg tatatccaac atgactttgc acaaaaaaca
     2521 gaatatcaac agccaatcgt tccatcagtg atgctgatgg ggatcatcac aagtgcaatt
     2581 tcaaagcatc ttcctggacc aggatcacat gttgtcaatt tttctgtcaa ttttattgaa
     2641 ccggttttcc attatgaaac actgactttt cagtttgagg tcataaaagt agataagatg
     2701 aaggatgtgg tgacgatttc tgtagaagcg gagaatacga acgaagatcg tgtgttggat
     2761 gctgtcgtta tggtccaacc gccacaagtt cctgaattgg aaatagcaat cgaaagtgaa
     2821 ggtgaattga atgaataatc caagtacatc aatgtcaggt atcaatcgtt tttatgcaaa
     2881 agtttatgga tttttaggta tgggacttgc ccttagtgca gtcacttctt acttggctct
     2941 ttgggtattt cctttgcaat tagcaacttt tatcaataat tttccactag gctttgtggg
     3001 tctgtggatc gttgaactga ttttagtaat ggttctaggc gtaaaagcac aaaagaatcc
     3061 gagcttagca atcggtggat ttattgccta ctctcttttg aatggcataa cattagcggt
     3121 tactcttgca tactacgata ttggaacagt aacgcaagct ttcttgacag ctactttgat
     3181 gttcatgggg atgtctgttg tcggagtaac aacaaaaaga gatcttagcg gggttggaag
     3241 agcaggacag gcagctttaa tcggagtgat cattgcacta gtcttgaaca tcttcatttt
     3301 gagaagtagc gtagttgagc tgttcatctc cctactgtta gtcgtcatct ttgcagggat
     3361 tacagcttat gataaccaaa gcatccgtaa tatctatgtc cagacaaacg gaacagcaca
     3421 tagtggaatt gctgtattca tggcattgaa gctgtatttg gactttatca acttgttctt
     3481 ggcgtttctg agaatctttg gacggaacaa ctaaaaaaga aaaaaattca tcagttgggg
     3541 ctataccgaa ttatttggta tggtcccgct ttttcttttc gcattccgca ggattctttg
     3601 ttagaatgaa taagaacttt acagtaagga gttaagaaga tgacaaaaaa taaattatta
     3661 ctcgttgatg ggaacagtgt ggcttttcgc gcattctttg cgcttcacaa ttctcttgaa
     3721 cgtttcaaaa atagaaacgg tcttcataca aatgcaatct atgcattcaa tacgatgttt
     3781 gaaaatgtta tgcaaaaaga gcaaccgact catgttctgg ttgcgttcga tgcaggaaaa
     3841 acgacttttc ggacagaaat gtatgcggag tacaaaggtg gaagatccaa aacacctggc
     3901 gaattcaaag aacaaatgcc atatatacgt gatttgttga caggactagg ggtacagtat
     3961 tacgaattgc ccaattatga agcagatgat attatcggta ctttggcaga aaaagtagac
     4021 aaagatcaat ttgatgtcgt cgtgctttca ggtgatcggg atttgactca attagctagt
     4081 aaagaagtaa aagtggatat cactgtaaaa ggtgtcagcg atatcgaaag ctatacacca
     4141 gaacatgtag cggaaaaata tgatgggttg acgccaaaac agattattga catgaaggga
     4201 ctagccggag atgcctctga caatatcccc ggtgtgacta aaatcggtga aaaaacagct
     4261 atcaagctat tgaaacaata tggctcagtc gaaggaattt atgaacatat cgatgaaatg
     4321 aagcaaagca aaatgaaaga aaatctgata aatgacaaag agcaggcttt cttatcgaaa
     4381 aaattggcga ccatcaatac ttcagcgcct gtggatgtct ctatcgactc attgaagtat
     4441 gaagggaaaa atctcgataa actcgttccg ttttataaag aaatggattt caatcagttc
     4501 ctttctaaat tgaatatcgt agaagaagaa gtaaaaatgg atgatatttt gtttgaagtc
     4561 gttcatgaat tcaaagagga aatgtttact acggatatgg cgttgtacgt ggaaatgatg
     4621 ggcgacaatt atcatacaga ggaaatcgtc ggagtagcgt gggggacaga aaagaaaatt
     4681 tatgtcacga acgatctatc tgtattcgaa aaccgagcgt tccattcttg gatcactgat
     4741 ccgactcggc tgaaaaaagt atatgatgcg aaacgaactt atgttgcttt aaaccgctat
     4801 actggaaaaa cgaaaggaat cgattttgat gtcttgctag gtgcatacct tctcgatacg
     4861 aatgaaaaaa gcacagatat cgaaggaata gctgctcatt acggatacaa cgatatccaa
     4921 tcagatgaag ctgtatacgg caaaggggcg aaaaaaggtt taccagaaga agaggaactc
     4981 ttttttgctc atttagcgcg aaaagtagct gcaatcaatg cgttgacacc aaaattgagc
     5041 caagaactgg cagaaaaaaa ccaagaagat cttttccgga aaatggaact gccattgtct
     5101 atcattttgg cagagatgga aataagcggg atcaaagtag atgcgactcg cttacaagaa
     5161 atgaagggcg agttctctgc tcgcttaaga gagatcgaac aaaaaatcta cgaagaagcc
     5221 ggcgaagaat tcaatttgaa ttcaccaaag caattaggag tgatcttatt tgaaaaaatg
     5281 ggattacccg ttatcaaaaa aacaaaaact ggatattcca cagccgtaga tgtactggaa
     5341 cagcttcgtg aacaagcacc aatcgtagag gatatcctca cctatcgtca aattgcaaaa
     5401 attcaatcta cctatgtcga gggactgctg aaagtgatcc aatcagatgg aaaagtgcat
     5461 acccgttatg tccagacatt gacccaaaca ggacgattaa gctctgttga tcctaatttg
     5521 cagaacatcc cgattcgatt agatgaaggg agaaagatac gtcaggcatt tgttcctaga
     5581 gaaaaagact ggctgattta ttcttctgac tattcacaaa tcgagttacg cgtacttgcg
     5641 catatctcaa atgatgaaca tttgaaagct gccttcctag aaggtcaaga tatccatgcc
     5701 agcacagcga tgcgtgtatt tggaatcgaa aaggcagagg atgtcacacc aaatatgcgt
     5761 aggcaggcaa aagcagtgaa ttttggtatc gtgtatggca tctctgatta cggactttca
     5821 caaaatctag gaatcagccg aaaagcagca cagcaataca tcgatacgta ctttgaaaaa
     5881 tacccaggtg tcaaagaata tatggaaaga atcgtacgcg aagcaaaaga ccaaggctac
     5941 gtcgaaacgc tgtatcaccg ccgtcgatat ctgccagaca tcaattcaag aaattacaat
     6001 atccgttctt ttgcggaacg aacagctatc aatacaccaa tccaaggaag tgctgctgat
     6061 attttgaaaa tcgcgatgat cgaattggat aaacgattaa aagaaacagg tcttcaagct
     6121 acgatgctgt tacaagttca tgatgaactt gtcttcgagg taccggaaaa agaacttgaa
     6181 tcattagata aattggtgaa ggaagttatg gaacaagctg tttctcttca tgtaccactg
     6241 atcactgaca gcagttgggg aaaaacgtgg tacgaagcaa aataaacatt gtttaagaca
     6301 gagcaatata atgaaggtga aaagaaaaaa tgccagaatt accagaagta gaaaccgttc
     6361 gtaaaggatt ggaacggtta gtagtaggaa aaacgattca aaaagttcag gtcttgtggc
     6421 ccaagatcat cgagcaacca gagacaccca tctttgaggc ttctcttgtt ggcgaaacca
     6481 tccaatccat tggacgtaga gggaagtttt taatttttca tctcgatcat tgtgaactga
     6541 tctcacactt gcgaatggaa ggaaaatatc aatttacgaa agaaaataca ccaatcgata
     6601 aacatacgca tgtacttttc ttttttgagg atggaagcca acttcgctac aacgatgtac
     6661 gaaaatttgg tcgtatgaca atcgtcgaaa aaggagcaag tgcaacttac cgaggaatca
     6721 tgaaattggg acctgagcct ttgccagata gcttcttatt agcagatttc gcaaatggac
     6781 tgaaaaagtc ccacaaagca atcaagccat tgctgctaga ccaacgtcta gtgactggtc
     6841 taggtaatat ttatgtagat gaagcattat gggaagcgaa gatccatcca gaacaaccgg
     6901 cgaacacact gcgccccaaa gaggtagaac aattacgtct atcgatcatt gatgttttgg
     6961 atcgtgcaat agaagccgga ggtaccacga ttcgcagtta tttgaatgca ttaggagaat
     7021 caggcggatt tcaagtagca ctgcatgtct atcagcaaac tggcaaacca tgtatacgtt
     7081 gtggaacgcc gatcgtgaaa acaaaagtgg cacaaagggg gacgcactat tgtccgaatt
     7141 gtcaaaggct aaaaatcgca aagtaggatt tgttttaggt ttgacgggtg gaatagctac
     7201 cggtaaaagt acggcagcca aagtctttca gtctcatgga tttcctttga tcgatggcga
     7261 tgtgattgcc agagaaactg ttgagcctgg aacgcttgct ctgaggaaaa ttgtatcagt
     7321 gtttggccaa gaggtattgc agcctgatgg gcaactagat cgagggaaac ttgggatgat
     7381 tgtttttcca tcaaaggaaa aacggcaaaa acttgatcag ctattagatc cgtttatccg
     7441 aaaagcgata aaagatcaaa tcgcctctct aagttccaat catcctttag taattgtaga
     7501 tatcccattg ctctacgaag gtcattatga ccactatatg gatgcagtag ctgtcgttta
     7561 tactacgcca gaaactcagc ttcagcgttt gatgaaacgt aatcaactga cgctagaaca
     7621 agcgcagcag agaattagta gtcaacttcc aatcgaagaa aaaaagcagc gtgcggatat
     7681 tttgttcgac aacaatggaa caaaagaaaa acttgtggga caaatcgaaa attggctttt
     7741 tgctaatcgc tttctttgag tatctgtgtc aattttgcta tcctgagtat atagaaagtg
     7801 agggatagcg atgggatcat taaaaaatca aaaggataaa gtcgtaggta acgcaaaaga
     7861 aaaaatcggc gagatgctca ataaagaaaa tctaatcgat gaaggtcgta cgcaatcaga
     7921 aacagcagaa gaaaaagaaa agcagtacca agcaagaatc gatgaaatag aagaccagca
     7981 ggcagaagaa gaagcgaagc ggcaagattt gactcaagaa cgaaaaaatc cgacacctga
     8041 tcgtagtaaa acaccgctcc cagaaagtga ccacattccc gatgagcaaa aaatcgagga
     8101 acagcgaatg aaacactata caggaatcga agagaagtta taaaatagta aaaaatcgag
     8161 atgtgacttg aagcagatga gaaggtgttc atctgcttca agttttttgt gctagatgac
     8221 taaatcagct tttgtgtaag ttatttctca gtaatcaagg tgatcagtat tggaaaattg
     8281 aaatttagta aggacagggg acacaattac gcatgaaagt agaaaaaatt gttcttatat
     8341 ccgtgttttt tgggtaggac agtcgcgttt gtgatttgtt tcgtgttata atacaactag
     8401 ttgtaaagaa agacacatcc tttatataaa atgcgatgca agaggtgaga caaaatgaga
     8461 tgtcccagat gtcaacataa taattcacga gtaatcgaca gccgtcaagc agatgatgga
     8521 cgtgcgatcc gtcgccgtcg cgaatgtgag aattgttcgt tccgattcac tacatttgaa
     8581 cgaattgaag ctgctccgtt attagtaatc aagaaaaacg gggaacgcga agagttcaat
     8641 cgcgataaaa ttttaagagg actcatccgt tcagcagaaa aaagacctgt agctatggaa
     8701 caaatggaac aaatcgtcaa ccatgtcgaa aaccgcgtac gcagtctcgg agaaaacgaa
     8761 gtatcgacga atctaattgg tgaatatgtg atggaagatt tagttgattt agacgaaatc
     8821 gcttatattc gttttgcaag tgtttatcgc cagttcaaag atatgtctgt tttcttaaaa
     8881 gagttgcaag atatcgtgga taaagcaaaa gaaaataata aagagaataa taaagaaaac
     8941 aattaagaaa caaaaaggag gcatttcctt ggaaaatgct tggaaagagc ttcaaccaaa
     9001 aaatatttac caaggaagaa aagcggctcc gctgagagaa gacagcaaga acgccttgct
     9061 gtatctttat cagccactaa tcggcggaga agccttgagt atctatttca ccctgttatc
     9121 agaaatctct caagaatctg gcaaaggtcc tgaagggatg catgcagatc tgcttagtag
     9181 tctaggctgc ggtatcccgc aattttatga tgcaagaaaa aagctagaag gaatcggctt
     9241 actggatgtt tattttaaag aagatagtga tctgggacct cgttttattt atgagctgtt
     9301 agaaccactc attcctaaag catttttcaa agactctgtt ctggcttttc ttcttctgga
     9361 aaaagttggt gaacgacgat tccagaagct tacccaattc tttgaaccta aaaggcatac
     9421 agcagaggga tatcaaaaaa tcacaaaaaa atttttagat gtttatcgct tcaatgaagc
     9481 agtgtacgct gctaatcaag aaaacatcga atcgctgaat aaatcttttg aagaatctga
     9541 atcagtagca ttacttgaag cagattcgac attcgattgg cagtttttga atgattggct
     9601 ggcaaaaaaa catattgcaa gtcttgatga tccaatcatc aaacaagtcg acatgtatca
     9661 tcagctttac ggtttcgatg aattgacact tggcgaactg atcagtcaat cttatgactt
     9721 tagtgaacaa aaagtatctg aaaaagaact gcaaaaagcc attttggcaa tgcagagacg
     9781 tccggtgaag caaacaaaag aaacagttgc ggtttctgaa aacagtaatg aaccctcgtc
     9841 aaacgaacat ttgacaaacg cagcgcttca gctgataaaa gaagcacaga gcattccacc
     9901 gatgaaatat ctggaagcaa tcaagtcaga aaaaggcgga tatgtttcta aacaagaaaa
     9961 ctggttactg caagatcttg tttctcaatc aggtctttcc agtagcgtga tcaatgtttt
    10021 gctgaattat gtactagtga tcaagaataa tgctagctta aatgccagtt atgtaaatac
    10081 agtggcaaat gaatgggcac aaaagaaaat cgccacagca gaagaagcga tccagcacat
    10141 tcggcaaata agtaatcaag caaaacaatc aaaacaaaag aagcaaacaa attatcaaac
    10201 tggcaagcga aatgttcgac gggaaaagct tcctgattgg gtcaaccagc caaaagatga
    10261 aaaacagatc agcagcgaga aaaaagcaga gattgaccgc cgttttaaag aatacctggc
    10321 aaaaaaggag ggtgatatct agtggaagac gtaggcaaag aattgaagag gatcatcagc
    10381 aaacgcaatt atcaagaaaa gtatgctgaa atgatcgctg aagtcttaaa tgatgcggat
    10441 gttcaagctt ttctaagcgc acaccaagaa gaattgacac aagcggatat tgaaaaaagt
    10501 tacgctaaat tgtatgaatt cgtgcaagaa aagcgtaaat atcaaaaaaa cgatccaacg
    10561 atgatcgctc ccggctatga gccgaagctg acgttgaatt ttcattatgt ggatgtcact
    10621 tatgtaccaa cggaagcatt gttagcacgg caaagacaag aagaaatcag aagccgagta
    10681 aaagcaatgg atatgccaaa agatattcaa tcggcttctt ttgaaaactt tgaacgtaca
    10741 gatggacgag gatatgcgag cttagaagcc ctagattttg tagagaaata tactgctgat
    10801 ccaaaagggt atcataaagg actgtatcta gtaggaagtt ttggtattgg aaaaacatat
    10861 ttattaggag caacagcgag agaattagcg attgctggat tcaccacgac gctagtccat
    10921 tttccgacat ttgccgttga gatgaaacag gcaatcggaa aagatcaagt ggcagaaaag
    10981 ttagatgcag tgaaacgttc acctattttg atgatcgatg acattggtgc agatgcgatg
    11041 agtagttgga tacgagacga agtattcggt gttatccttc aatatcggat gcaggaacag
    11101 ttaccaacct tcttctcatc gaattttaca atggatgaat tggaacagca tttgtccgtc
    11161 actcaacgtg gagatgaaga gccgctaaaa gcgaaacgga taatggaacg catacgttat
    11221 ttgacaaaag aaatcgaaat gactggtcgc aaccgacgca atggttgaac agttgacaag
    11281 tccaactgac atttgaccta gtagtaaaag caggtcagcg aatcattata tggacagaaa
    11341 tgaccggaga caatcaccgc aacaagtaat gcagaaactt aaacggagga ttttttatga
    11401 gggctatttt ttctagtata tctgtagttg gtctattttt tcttttagca ggctgcggta
    11461 gcgcagatag caacacaggt tctgttgatt cgattgatgc gggtcaaaca gtaaaaacaa
    11521 gcagtacgaa aacggagaga caaagcatat ttgtcggaac gattgaaagt atcactcaag
    11581 tagaagcaga agaagaaacg cttcagttga aactcgtcga tgtcatggcc aaagaagatc
    11641 cagcgaatat gggaacgtca ttcagcaatg acggagtgat cttaaatgct actgctgaaa
    11701 aaataagcgt ggacataaat gaattgaaaa tcggtgacaa agtccaattt acattagttg
    11761 agatgccgat catgacgatg tctatcccac cgcaagttcc tggagattcg attattgatg
    11821 tttcattgaa caaataacca ataacatttt ttcaaaaata agaaaatgtt gttttataga
    11881 acgaaaa
//
LOCUS       DASJON010000085        11878 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJON010000085 DASJON010000000
VERSION     DASJON010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.085"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(153..2228)
                     /locus_tag="U3989_001660"
     CDS             complement(153..2228)
                     /locus_tag="U3989_001660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN1715112.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2230..3147)
                     /gene="glyQ"
                     /locus_tag="U3989_001661"
     CDS             complement(2230..3147)
                     /gene="glyQ"
                     /locus_tag="U3989_001661"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN1715113.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3533..4351)
                     /gene="recO"
                     /locus_tag="U3989_001662"
     CDS             complement(3533..4351)
                     /gene="recO"
                     /locus_tag="U3989_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN1715114.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4494..5393)
                     /gene="era"
                     /locus_tag="U3989_001663"
     CDS             complement(4494..5393)
                     /gene="era"
                     /locus_tag="U3989_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN1715115.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5408..5803)
                     /locus_tag="U3989_001664"
     CDS             complement(5408..5803)
                     /locus_tag="U3989_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN1715116.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5787..6263)
                     /gene="ybeY"
                     /locus_tag="U3989_001665"
     CDS             complement(5787..6263)
                     /gene="ybeY"
                     /locus_tag="U3989_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN1715117.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6292..8484)
                     /locus_tag="U3989_001666"
     CDS             complement(6292..8484)
                     /locus_tag="U3989_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN1715118.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8508..9479)
                     /locus_tag="U3989_001667"
     CDS             complement(8508..9479)
                     /locus_tag="U3989_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN1715119.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10184..11563
                     /gene="brnQ"
                     /locus_tag="U3989_001668"
     CDS             10184..11563
                     /gene="brnQ"
                     /locus_tag="U3989_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1715120.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3234 a         2531 c         2173 g         3940 t
ORIGIN      
        1 ttatttcctc acttttcatg agcgttttga aaattcgaac aaagaataaa ataacaaaaa
       61 ttattaaaat agaattaata acagctattt ataacaaaaa aaacagagac acagcacgtt
      121 tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg tgatgatttg atccaagctt
      181 gctaatgaca aagccatggc agctatttga cttagttgtg ttagtcggtt cttacgtact
      241 gctggttcat cagccatgac catcgtttga tcaaaataat tttcgatcag tggacgaaga
      301 gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag caaaacttgc tttcattgct
      361 tctactgctt tgtgtaattc tttctctgcg tcgttttcga acagctcagg gtctacttct
      421 gattgttttt caagctgcgc ttttttcgct aagttcacga cacgtgtcaa agcttccatt
      481 gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca attgtgctgc tgcaaatact
      541 tttgtcagat ctttttgttc cgcattcaaa acagcatcga tgacatcatg acgaatattc
      601 ttcgttgtca ataattggcg cagacggcct ttgataaagt cgatcacttg ttgttgccct
      661 tctgataaac caatgccgaa acgatccgta tctttactga taacatcgct aatcgtttct
      721 tgcagaacag acaaaggaaa ggcccatttt ttcgattcga tgatccgaac gacaccgtat
      781 gcttggcgac gtaaggcata aggatcgttt gacccgcttg gaatcatccc aactgcaaag
      841 aaagaaaaga tgctatctaa tttatctgca atcgctaaaa cagaacctac tgtagactga
      901 ggtagctctc cttcactgga tataggcatg taatgctcac gaatcgctgt cgctacagca
      961 ggtttctcgc ctttcagaag agcatatttt tcgcccatga tcccttgtag ttcaggaaat
     1021 tcccctacca tgttggttac tagatcaaat ttatagattt cagcagctcg ttgcaaatcc
     1081 atcaattctt cctcagatag tcctacttgt tcgccaatga tacgggcaat gacttgtacc
     1141 cgttgcattt tctcatagat actaccgatt ttttcatgga aagtaacagt ttttagtttt
     1201 tcaacgtatt ggtcgattga agctttcttg tcttcattat aaaagaattc cgcatcttct
     1261 aaacgagccg tcaatacttt ttgattgcct cgaaccacat cttccaattt tacccgatca
     1321 ccattacgag cagaaataaa atgaggaagc agcatacctt gatcattccg tacctcgaaa
     1381 taacgttgat gttctttcat agaagtgact agtacttctt caggaacaga aagatatttt
     1441 tcttcgaagt cgccagaaaa gacagttgga tattcgacta aattgttgac ttcttctagt
     1501 aaatcttcgt ctagatcaat aacccagtga tttttttctg ctaattcgtt tgcttgtgta
     1561 acgatcattt cttgtctttt ttgcggagac gcaatgacga attgttcatt caatttttct
     1621 tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta agaaacgatg acctcgagag
     1681 gctctgcctg ttttgacatc caagatttcg aatgggatta cttcgtcatc taacaatgcc
     1741 acgatccagt gaatagggcg gatatattca aaatcgtatt ctgcccaatg catcgttaca
     1801 gggaaagtca aacttttgat cacccctgct aagcctgtca atacttcttt tgcttcctta
     1861 ccatgagtgt atttcgttac atagacatat tccacacctt ttaattctct aaaagtgata
     1921 gcgtctgtcg tcagaccttg tccgcggaca aatccttccg ctgctttcgt ccagtttcca
     1981 tctgcgtcca aagcaatttt tttggctggt cctttgactt cttcttcact gtccgcttgt
     2041 cgttctggaa tatttgttac tttgattgct aaacggcgcg gtgtggaaaa tgtttcgatc
     2101 gtatcataag tcaattggtt ttcatctaaa aattttgctg ttttttcttc taattgtttc
     2161 atgcttggtg tgacaacatg ggcaggcatt tcttccagcc cgatttcaag taataagtct
     2221 tttgccatct tattcagcct ccttcacggt ttctggattt gctgtgtttt tattcaacaa
     2281 agggaatcct aatttttcgc gttctgccac aaagattttg gctacagcac gagccatgtt
     2341 gcggatgcga gaaagataac cggcacgctc tgttacggat actgcaccgc gggcgtctaa
     2401 taagttgaat gtatggctac atttcaagat ataatcatac gccggatgaa caagattttc
     2461 atcgatacag cgtttagctt ctttttcaaa acgatcaaag ttgcttagta gcaagtcttg
     2521 gtcgcttact tcaaaagaat atttggaatg ttcatattct ggttgtttga agatctcgcc
     2581 atatttcacg ccatcagccc attctaaatc atagacactt tcaacttctt ggatataaga
     2641 agcaagacgt tctaatccat aagtgatctc agaagttact ggtttacaag gcagtccacc
     2701 aacttgctgg aagtacgtga attgtgtgat ttccattccg tcaagccata cttcccaacc
     2761 cacaccggca cagcccatgg atgggttctc ccagttgtct tcaacaaaac ggatgtcgtg
     2821 ttctaatgga tcgattccta aaaggcgcaa gctttctaaa taaagctctt gaatattctc
     2881 aggtgaaggt ttcatcacca cttggaattg atgatgctga tacaaacggt ttgggttttc
     2941 accataacgt ccatcagctg gtcggcgaga tggctctacg taagcagcat tccatggttc
     3001 tggtccgatt gcccgtaaaa aggtatatgg gctcatcgta cctgctcctt tttctgtgtc
     3061 ataagcttgc atcagcatgc agccgtttga tgaccaaaat ttttgtaatg ttaaaatcat
     3121 atcttggacg gtcaattttt tactcatctg ttttgcccct ttcaatttta aagcgttgct
     3181 gtcaaataag aatacaggaa agaaaacgac aaaaaatccc tatgtctgat tacagacata
     3241 gggacgaata attcgcggtt ccaccctact tccgaataaa gattcggcag cttcatttat
     3301 cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg ctcccactct cccgaactcg
     3361 ctaaaccacg tgttttttac tgatcttggt caatgtacgt attcattttg cagttttctt
     3421 gtttctataa tagatcctat cactgaacga tctatttaat ctatggttaa taatagacgg
     3481 aactccctgt gtttgtcaaa tggttttgat tttttgttgt atgttttatt tactattctt
     3541 ctttttcagg tgtctctttt tctttttcag acatgtcttt ctctgacgtc tttaatacgt
     3601 gtgcccaaga cttcatttga ttgatgaact ttttgctttt caaatggata ccgacatatt
     3661 catcatacag ctggtcgatg agttcacgga ttgcttgttt ggtttcttct ttcagatgga
     3721 ttccactaat tttatcataa gaaatagctg aaaacatccg aacgaaataa acagctcgag
     3781 gatctgcatg ataacggtga tcgtccatcg accaatgtct ttcgcatatc acaccaccat
     3841 atttggaaga atagtcaaat tttccttttg tttccccgca tacgacgcag tgtgtccaga
     3901 ttggtgcaat tccgaagcgt tgcagtattt ggacttcaaa aatattcgtg atgatttctg
     3961 catcgtctcc ttggtccatc cgttgcagtg cttgttgcgt aaactggaac aaatgcgggt
     4021 cgtactgatg atcttcgatt gccgcatcta ctagattcaa taaatatgtg ccatagccgc
     4081 tcaaaaagat gtcttcttgg atgtgtcgaa acgcatggac atctttgaca ctgcttaaaa
     4141 atgacaaacc ttcacttcga aaattcccga tataaactgc ttcagtaaaa gggagcaaag
     4201 ccggagctaa gggctgattt tgccgatgga tctctttgat ataaaacatc attttgccag
     4261 aagattctgt aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa
     4321 tcaagccttt tgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa
     4381 aagcagattc tgccgatcca gatcatttat gttgcgatct tccgcagctt ttctcaacac
     4441 gcggattttt attcagttta tttctatacc taatttacaa gaagctcaga atatcagtat
     4501 tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt tgtctcgcca gtctttttga
     4561 actttgaccc aaagttctag aaagactttg tctcctaaaa gattttcgat atcttttctt
     4621 gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat ccctttttgg
     4681 ctatctcttt cgacaataat cgtagcctgg atatggattt tatcattctc gtttcgcttc
     4741 atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact
     4801 ttctcacgga tcaattcaga aacgatgaag tattcaggat gatcagtcac ttgatcatct
     4861 ggaaaatatt gtgggccctc tggcatttca ctgactaata catccatcaa acgttcgacg
     4921 ttgtttcctt ctgtagcaga gattggaatc acttgtgcaa aaggcatctg cgttgaataa
     4981 tcttcgatga ttcccaacaa ctcgtctgga tggattttgt cgattttatt gataatcaga
     5041 taaacaggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccgaccgcgt
     5101 ttttgatcag cactaatcat aaataagacc gcgtccactt ctttcaacgc actgtaagct
     5161 gcttctacca taaaatctcc taaacgatgc tttggtttat ggattcctgg tgtatcaata
     5221 aaaatcaact gagcttctgg tgtagtgtat actccttgga ttttatttct agttgtctgt
     5281 gccttgtcac tcatgatggc aattttttga cccacgattc gattcaataa tgtggatttc
     5341 cctacatttg ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatatgtt
     5401 ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc aacccaacga
     5461 tgacggtaaa acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc
     5521 caatcggatg aaaatgaaaa tccgtaaaca tgtccacgac attttcaaaa acagtattga
     5581 tgatctctac tatccatacg agaaaaacag ctaaaagcag ccataaccat tctatccgat
     5641 caagttggaa aatcacgccc ataaacaaag cgaggattcc gaacgcaaca tgtttcctca
     5701 tgttgcgttc ttcctcaaaa acagttttga ctccttggac ggcaaattcc acggacatca
     5761 ggaaatgttt gtttttctct acgtttttat cttttaagtc cataggcatc taagatctct
     5821 ttctgtaaac caaacatttc tttctcatcc tctggtgtca tatggtcata cccgttgata
     5881 tgcaagaatc catgaaccgc taaaaagcca agttcccgat catacgaatg gccgtaagct
     5941 tctgcttgct ctgcagcacg ttcagtagag atcatgatgt ctcccagtac acgaggcaat
     6001 ggattctctc catcttcaaa aatcactggg atttcgtctt cgccctcttc ttccaaggca
     6061 aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt
     6121 gctgcattgt ccatgaaagt aacagacatt tctgtttctt ctggcaactc taaatatgat
     6181 gctgcaaatt gcagcaagtc gtcgatttct ttcattttct cttctgagac ttggtttgtt
     6241 tcatccatga aggtaatatc cattatacat cgcttctttc tattcttctt gctattcttc
     6301 ttgttcttct tttatcttct caacttccga tttcatttta ggatatttga tccgagaatg
     6361 gaaggtactg cttaaaccgc tgatcaatga tttttctact ttacggattt cttttaacgt
     6421 caatccactg tcgtccaatt gtccatcgga aatacgctct tcaataagat tatggacaaa
     6481 actttcgatt ttttcgctcg ttggatggtc cattgcccgt actgccgctt cacaactgtc
     6541 agctatattg acgattcctg cttcacggct ttgaggtttt ggtccaggat aacggaattg
     6601 ctcttccgta acttccgggt tgcgttcctt tgccttaacg tagaaataac gcatcagcgt
     6661 ggttccatga tgctgtctac aaatatcgat caccatttgc ggcatattat aatcttccaa
     6721 tattttggca ccatctgtca catgtccaaa gataatatgt ttactgtctt ctggcaataa
     6781 gaaattgtgc ggattttccg ctcctgaagg caagttctca acgaaaaagc tggcgtgttt
     6841 gatcttgccg atatcatgat aatagcaagc tactcgagtt agtaaagaac gtccaccgat
     6901 ttctgctaca gcattagcac ttagattggc taccatcatg ctatggtgat acgtacctgg
     6961 tgcttcttcc agcaactgtt tcaataaagg atgattggga ttgctgagtt catttaatac
     7021 gatcacacta tcatcattga ccatcaattc aatatagggg tgcagaccta tcgtcagcaa
     7081 gaatgaaaac agtgcacctg tgaatccgca gactagcgtt aaccaagtcg ttgcatcccc
     7141 aaagctcatt ccttggtaga taatcaaaat gacatccatc atcacaggaa aaataatgac
     7201 ccacatagca gcggataacc cttgttctga gatccgtttg cgtctgacga tcgttgccat
     7261 gagaccagaa aatacatagg acatcaaaat aactgtcaga aagttcgtgc caatcgaatc
     7321 ataaaaaatg aaaagtgcgg atactgcttg aaaaatacca gccatgatcc ctgctcttcg
     7381 attcaagaaa aagcttaaaa caagtggagc aaaagccgcg gggtaaaaca gcggaatata
     7441 agcagcttgt tctgtttgga agagttggaa aaatttcatt agtacgacgc ttactgacat
     7501 tgccgtcacg tagaaaagta cgtattttgt tctttctcct gcattttgga attgaagtga
     7561 attataaatc agtacaccga tttgaagcaa aacagctaaa accatggcaa tgagtgggaa
     7621 aatagaagtc gtttgattcg tcaatcctag taattccaat tttttcatcg ctgaagcatc
     7681 aatttgattt ccttcgcgaa caataatttc tccttggaag atcatcactg gttgtacagc
     7741 ttctcgtgca gattgtttta attcttctgt ctttttctca ttcaatacat cattaactac
     7801 gatgccttga tccactaagt agcgaatcat ttgttgctgc tctgttgtga cattcaaata
     7861 ttgaatctga tcttctgctt cttgtttaaa agcttccaat tcgctttcac gaacttgttt
     7921 actcatctgt tcgtcaatga gcttcaaact ttcatcccta actgtgtgaa tttccgtttc
     7981 agtcatttca aatatcgtcg aataaaaatt gttcggcagt ttttggtaaa acgcaacgtt
     8041 ttctgcattc acgttttcaa aatttttctt cagtgtagca atccgctctt ctgtagtagg
     8101 tttcgggata ttttcatctt ccttcgcctt atctaccttg ccattataag atttgttgat
     8161 ggtatcattc gtcttatcga tcaattcgaa taattgcttg atccgattgt tctggtcatc
     8221 tgccagatct ttttgataag tatattctgg agtcactgct tctgctgcca gttttcttct
     8281 ttgttcggtt tcttccgtgt tctcaatcgt tttattcgca cggatactct cttctgctac
     8341 ttggccttct ctgaaatcaa tggccttttg tttgacacta ctaaatgtaa cgccgcagac
     8401 aatgataaaa aacaagaaca gaaggaaagg caatgctgcc tttccgagtt tttcttgtag
     8461 tttctgcagt gttttattca acatgtgctt atcctcctaa gcaaagctta ttcttgatga
     8521 agatccgcct tttcataagc tttgatgatt tcggcaacta cagggtgccg aacaacgtca
     8581 cttgcttcaa aattaacaaa atcgattttt ttgatttgct ttaatgttcg ttctgcatga
     8641 acaagaccac ttgtcgtccc acgaggcaag tctatctgac ttgtatcccc attgacaatc
     8701 atttttgatt gataaccgag acgggtcaag aacattttca tctgagcgac tgtcgtgttt
     8761 tgtgcttcgt ccaagataac gaatgcatca tccaatgtcc gcccccgcat gtaagctaaa
     8821 ggtgcgattt cgatcacgcc tcgttccatc aatcggttcg tatgatccaa tccaaaaatt
     8881 tggtataaag catcataaac aggccgcaga taaggatcca ctttttcttt cagatccccg
     8941 ggtagaaagc ctaggttttc ccctgcctcc acagcgggcc gcgtcaaaat gattttttgc
     9001 acttcgcctt tttttagggc agcaattgct aatactacgg caagaaacgt tttccctgtt
     9061 ccggctggcc ctacaccaaa tacgatgtca tgtttggcta ccgattcaac gtatttcttt
     9121 tgaccgctgt tttttacccg tataggtttt ccattacggt ctttgacgat ctcttcctca
     9181 tacatctcaa caaaataatc taatgtacct ttttgcccca ttttcaatgc cgtcacaaca
     9241 tctggcgtac ttacgtggat tcctcgtttg atcaattcct gtaatgtccg aatgactgag
     9301 gcagccaagg aaatctggga ttgctcacca atgatctgaa tgatttctcc acgtgtatgg
     9361 atggttgttt ccgttgtttc ctcgatcagt tttatatgct tgtcgtgtgc acctaaaagc
     9421 atgctgacat catcagcagc tgtcaatcta atctctaaag aactagactc ttccgtcaaa
     9481 aatagacatc cccttttttt cgattcattc tttcctattt taccatactc agaagaaact
     9541 atatagcaaa taagaaaagg ttgtaaaaga gtcgcttaac catacgtacc aagaaacgtt
     9601 aaaaaatagc cgtttccatt cgctaaaggt ctcgttttga taaaaagaag ctgagaatgt
     9661 aagttatatg aaagtggaga acaaaatatt tcgtttcatt caacaagtat tctctaggta
     9721 tttttcctgt ttcaaaaaaa gaaaacaaaa tagttaaaat attcttgcat ttcaaaaaaa
     9781 acaggtgtat tattacgtca acaaataaac atacagcaaa gcgtcgagga aaacagtagg
     9841 ctattcaacg atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag
     9901 acgaacatca tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc
     9961 aggctagcgg ataactttat caaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa
    10021 aaaggtggta ccgttctaga atctagagcc cttttcccaa gtggaaaagg gctttttttt
    10081 attttaaggt ggtggttttc atggaatctg atattagtgg cattactaat tgctgcaacg
    10141 tttatatcgt caatcaaaat gataattatt ggaggaaaaa tttatggaag aaacaaaaac
    10201 ttattcatgg aaacaactat tgatggtcag ttcacttatc ttcgggatgt tcttcgggtc
    10261 aggtaatcta atctttcctg tccacttagg tcaaatggct ggcgcaaact gggtcagtgc
    10321 tggcgtagga tttgctatct caggtgctct tttcccatta ctagctatct tagctgtagt
    10381 agtgacaaaa agtgatggtc tgtatgactt agctaaacct gtcggaaaat ggtatgctgc
    10441 cttgttctta atccttgttc atttgacgat cggaccattt ttcggtacac cacggacagc
    10501 tgctactgct tttgaaatgg ctgctaaacc atttttgcct gaaaaatata attcattagg
    10561 tatgttttta ttctctgctc ttttcttttt acttgcttat ttccttactg tccatccaac
    10621 acggttggtc aaatacgtcg gaaagtatct aaactccttg tttcttgcat tattagcaat
    10681 catttttgtc gtttctttat tccatccaat gggaaatcta aatcaatctg ctgcagatac
    10741 ttatcaaaca aacgctttaa caaatggtgt cctagaaggt tataacacgg ttgatgcagt
    10801 agctttgctt gccttaagtg tcacttttgt ccatgcagtc cgtgaccttg gtttcaaaga
    10861 taaaaaagtc acagaattaa cggcaaaagc tggtagcctg agcgttgcgc tagaagtcat
    10921 gatctatttt ggacttgttc ttctcggtgc gatgagtttg aataagatgt ctttatcaga
    10981 aaatggcgga actgcacttt ctcagatcac taatttttat ttaggtaact tcggcgctag
    11041 cttcttagga gtgatggtca cattaggtgt cttcactacc gctatgggac tcgttgtttc
    11101 ttttgcgcaa gatttccata aactgttccc taaagtcagt tatatgacat ggctgcggtt
    11161 aacgactttc gtctcttttg tcgttgcaaa tgctggttta gacaatatca tccaatggtc
    11221 tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg ttgatcttgc tttccttaac
    11281 agcaaaattt ttccaaaaga caccatttgt ctatcaagta acgatgttgt ttgctgctgt
    11341 tcctgccgta ttggatatgc tggcaaattc gcctgcttta gtgagtcaac aaagagttgt
    11401 agcaagcatg ctcgaatttt atcatcatca cgttcctttt gctgcattag gacttggctg
    11461 gatggtccca acgttacttg gttacgctgg cagcctactg ttttattacg cctatcgcct
    11521 atcagggtat aaacaagaag caaatgaact accagaagaa taaccaaatc aaacacaatt
    11581 taacataaga aatattaaac aggaaaaaac cagctagatg aaagaatcct attctttcgt
    11641 ctagctattt tttagttctc ttttttacgc gcacaaaaaa actgcggaca atgattgtcc
    11701 gcagtttaaa tgggctctat aatatctagt gttggtgacg ccttctgttg ccaatacttt
    11761 ttcttttttc taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa
    11821 aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactag
//
LOCUS       DASJON010000086        11798 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJON010000086 DASJON010000000
VERSION     DASJON010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.086"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..283)
                     /locus_tag="U3989_001669"
     CDS             complement(32..283)
                     /locus_tag="U3989_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN1715121.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            430..1053
                     /gene="lexA"
                     /locus_tag="U3989_001670"
     CDS             430..1053
                     /gene="lexA"
                     /locus_tag="U3989_001670"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN1715122.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            1233..1451
                     /locus_tag="U3989_001671"
     CDS             1233..1451
                     /locus_tag="U3989_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715123.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(1740..2252)
                     /locus_tag="U3989_001672"
     CDS             complement(1740..2252)
                     /locus_tag="U3989_001672"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN1715124.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(2266..4563)
                     /gene="recJ"
                     /locus_tag="U3989_001673"
     CDS             complement(2266..4563)
                     /gene="recJ"
                     /locus_tag="U3989_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN1715125.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(4705..5148)
                     /locus_tag="U3989_001674"
     CDS             complement(4705..5148)
                     /locus_tag="U3989_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN1715126.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(5149..5937)
                     /locus_tag="U3989_001675"
     CDS             complement(5149..5937)
                     /locus_tag="U3989_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1715127.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(5934..6875)
                     /locus_tag="U3989_001676"
     CDS             complement(5934..6875)
                     /locus_tag="U3989_001676"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN1715128.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(7052..7639)
                     /locus_tag="U3989_001677"
     CDS             complement(7052..7639)
                     /locus_tag="U3989_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN1715129.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(7860..9167)
                     /gene="obgE"
                     /locus_tag="U3989_001678"
     CDS             complement(7860..9167)
                     /gene="obgE"
                     /locus_tag="U3989_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN1715130.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            9461..10471
                     /gene="gap"
                     /locus_tag="U3989_001679"
     CDS             9461..10471
                     /gene="gap"
                     /locus_tag="U3989_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1715131.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(10556..11491)
                     /locus_tag="U3989_001680"
     CDS             complement(10556..11491)
                     /locus_tag="U3989_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1715132.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            11677..>11798
                     /locus_tag="U3989_001681"
     CDS             11677..>11798
                     /locus_tag="U3989_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715133.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         3314 a         2625 c         2056 g         3803 t
ORIGIN      
        1 acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc
       61 cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc tcaggatcga
      121 cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc aaatactctt
      181 tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc ttctttttac
      241 gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc cctcctttta
      301 ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta aataaaatac
      361 gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa gtgaaagaag
      421 gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg atatatccat
      481 gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga agctgttcag
      541 ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc
      601 caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt
      661 gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga acccatttta
      721 gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc cgaagaaggc
      781 agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat attggatggt
      841 gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt cattgcaatg
      901 actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt tttccgtctg
      961 cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat attaggacga
     1021 gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag aaaacttttt
     1081 aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt cttctttcaa
     1141 atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa tatacgaaca
     1201 attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt cttggcttag
     1261 tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt ccagctttac
     1321 ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc
     1381 aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac tacagatcca
     1441 ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta aacttttacg
     1501 ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc taaagccagt
     1561 ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc
     1621 ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg
     1681 agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct
     1741 taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc
     1801 atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg
     1861 atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga
     1921 cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa
     1981 gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact
     2041 cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc
     2101 atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc ccgataagcg
     2161 tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg
     2221 ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca
     2281 tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag
     2341 ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg
     2401 taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat
     2461 taataatttt tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc
     2521 aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc ttgtgcctac
     2581 cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat tgaatgaata
     2641 agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa caactagaga
     2701 gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc
     2761 agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag
     2821 gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg
     2881 cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc
     2941 attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt
     3001 accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga caaatttcag
     3061 atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa
     3121 ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc
     3181 tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa
     3241 gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc
     3301 acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg
     3361 catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc
     3421 tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt
     3481 cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg
     3541 attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct
     3601 ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt
     3661 cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg
     3721 agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc
     3781 aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc ctaatgaaac
     3841 aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa
     3901 atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga atgctactcc
     3961 gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc
     4021 ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc
     4081 agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat
     4141 caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc catacccatg
     4201 ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt
     4261 cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc
     4321 tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt gtatcagata
     4381 cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg
     4441 gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat
     4501 ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct
     4561 cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa
     4621 gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt
     4681 acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg
     4741 agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc
     4801 cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc
     4861 ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag
     4921 ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag
     4981 tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa
     5041 gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac
     5101 taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt
     5161 aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc
     5221 aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat
     5281 catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc
     5341 gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca ctgtcgtaac
     5401 agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa
     5461 tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca tagaggcgat
     5521 attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac
     5581 catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag
     5641 gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc
     5701 ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa
     5761 agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc
     5821 cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg
     5881 tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc
     5941 tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt
     6001 tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca
     6061 ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga
     6121 tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt
     6181 tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta
     6241 tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg
     6301 atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt
     6361 ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt
     6421 tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa
     6481 aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa
     6541 cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc
     6601 ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga
     6661 agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga
     6721 cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact
     6781 tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga
     6841 acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc
     6901 ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa
     6961 ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc
     7021 gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt
     7081 cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat
     7141 tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgagtaa
     7201 tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag
     7261 caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg
     7321 tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc
     7381 atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg attaatccac
     7441 ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg
     7501 tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat
     7561 gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa
     7621 tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc
     7681 tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac
     7741 aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct
     7801 gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc
     7861 tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg
     7921 cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact
     7981 gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag
     8041 atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca
     8101 tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct
     8161 aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt
     8221 ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg
     8281 aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc
     8341 ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct
     8401 tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt
     8461 ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc
     8521 aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga
     8581 ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg
     8641 gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact
     8701 ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc aatttccggt
     8761 gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc
     8821 acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct
     8881 cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc
     8941 cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc
     9001 atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct
     9061 ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct
     9121 ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag
     9181 tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt
     9241 acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct
     9301 tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt
     9361 tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat
     9421 gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat
     9481 taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga
     9541 tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca
     9601 atttgactct actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt
     9661 agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc
     9721 aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa
     9781 agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc
     9841 tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat
     9901 ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga
     9961 atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct
    10021 cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac
    10081 tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg
    10141 aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt
    10201 ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac
    10261 agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg
    10321 aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt
    10381 ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat
    10441 tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct
    10501 ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat
    10561 gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt
    10621 atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac
    10681 gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga
    10741 gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc
    10801 cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa
    10861 acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa
    10921 tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc
    10981 ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct agcactttgt
    11041 ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc
    11101 tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct
    11161 ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt
    11221 tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag
    11281 tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt
    11341 tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa
    11401 cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga
    11461 cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca
    11521 tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat ttttataacc
    11581 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
    11641 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
    11701 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
    11761 aaccgaatgg gacgtgctat acaaactatt tataatgt
//
LOCUS       DASJON010000087        11706 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJON010000087 DASJON010000000
VERSION     DASJON010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.087"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..586)
                     /locus_tag="U3989_001682"
     CDS             complement(5..586)
                     /locus_tag="U3989_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1715134.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="U3989_001683"
     CDS             complement(769..1395)
                     /locus_tag="U3989_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1715135.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="U3989_001684"
     CDS             complement(1417..2748)
                     /locus_tag="U3989_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN1715136.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="U3989_001685"
     CDS             complement(2903..3304)
                     /locus_tag="U3989_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1715137.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="U3989_001686"
     CDS             complement(3390..3596)
                     /locus_tag="U3989_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715138.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="U3989_001687"
     CDS             complement(3691..5193)
                     /locus_tag="U3989_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN1715139.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="U3989_001688"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="U3989_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1715140.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="U3989_001689"
     CDS             complement(5764..6381)
                     /locus_tag="U3989_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN1715141.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="U3989_001690"
     CDS             6602..7975
                     /locus_tag="U3989_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1715142.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="U3989_001691"
     CDS             8050..9162
                     /locus_tag="U3989_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN1715143.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="U3989_001692"
     CDS             complement(9234..10871)
                     /locus_tag="U3989_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN1715144.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="U3989_001693"
     CDS             complement(11039..11647)
                     /locus_tag="U3989_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1715145.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3074 a         2470 c         2121 g         4041 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 cattta
//
LOCUS       DASJON010000088        11621 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJON010000088 DASJON010000000
VERSION     DASJON010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.088"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..439
                     /locus_tag="U3989_001694"
     CDS             5..439
                     /locus_tag="U3989_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715146.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(517..1050)
                     /locus_tag="U3989_001695"
     CDS             complement(517..1050)
                     /locus_tag="U3989_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN1715147.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1144..2322)
                     /gene="mutY"
                     /locus_tag="U3989_001696"
     CDS             complement(1144..2322)
                     /gene="mutY"
                     /locus_tag="U3989_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN1715148.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2315..3112)
                     /gene="recX"
                     /locus_tag="U3989_001697"
     CDS             complement(2315..3112)
                     /gene="recX"
                     /locus_tag="U3989_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN1715149.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3219..4601
                     /gene="rlmD"
                     /locus_tag="U3989_001698"
     CDS             3219..4601
                     /gene="rlmD"
                     /locus_tag="U3989_001698"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1715150.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4775..5353)
                     /locus_tag="U3989_001699"
     CDS             complement(4775..5353)
                     /locus_tag="U3989_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN1715151.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5675..6145
                     /locus_tag="U3989_001700"
     CDS             5675..6145
                     /locus_tag="U3989_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN1715152.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6253..7479)
                     /locus_tag="U3989_001701"
     CDS             complement(6253..7479)
                     /locus_tag="U3989_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1715153.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8233..8433)
                     /locus_tag="U3989_001702"
     CDS             complement(8233..8433)
                     /locus_tag="U3989_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1715154.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8567..9175)
                     /locus_tag="U3989_001703"
     CDS             complement(8567..9175)
                     /locus_tag="U3989_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN1715155.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9260..9730)
                     /locus_tag="U3989_001704"
     CDS             complement(9260..9730)
                     /locus_tag="U3989_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN1715156.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9778..10692)
                     /locus_tag="U3989_001705"
     CDS             complement(9778..10692)
                     /locus_tag="U3989_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1715157.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10838..>11621
                     /locus_tag="U3989_001706"
     CDS             10838..>11621
                     /locus_tag="U3989_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN1715158.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGW"
BASE COUNT         3493 a         2311 c         1900 g         3917 t
ORIGIN      
        1 gaccatgaaa gttgtacaaa caaaagatac aatgagcaat atttacttag atgctgtacg
       61 gatcaggcat caagtttttg tagtcgaaca gggtgtgcct ttgtctcgag aaatagacaa
      121 agatgaagcc cattgtatcc acttcgtcct gtactcagat aaaaaagaac cgcaaggaac
      181 tgtacgtctt ttgcctttag aaaacggaaa aatgaaatta cagcggatgg cgattctttc
      241 tgaatacaga caccaagggt taggaaaaat attgattgaa gaagcagaaa actttgctaa
      301 gaatcaaggc tataatacga ttttattagg ggcacagtcc acagctgaaa ctttttacga
      361 aaaactagga tatacagctt acggcgatcc ttttgaagat gctggcatgc ctcatattta
      421 tatgaaaaag actttataaa aatttatttc gaaaaaagca aaaaacagca agcttttgag
      481 aaaaatctct cactggctta ctgttctttt ttgtgtttac ttacgcgaca attgcttgta
      541 acggttatac caaatatcaa tatagccttc cgagaacggg ccatctccat tattgatcca
      601 gtcaaccaga atcttcacat tttcttttag gatgaagtca atatcgttcg ggtaattcat
      661 cattttgctg tgcatctcat attcatcgac atccaatagt cgtttctcgc cgtcaggaaa
      721 taccttgata tccaagtcat agtcgatata tttcaaggcc tcatcgtcca gcaaaaaagg
      781 tgaagctaga ttacaataat aggaaacccc cttttctctg atcattgcta ttacattgaa
      841 ccagtatttt ttatgaaaat aaacaatcgc tggttcgcgc gttacccatc ggcgtccatc
      901 agattcagtg actaatgtgt gatcattcac gccgatcata gagtgttcgc ttgtttttaa
      961 taccatggta tctcgccacg ttcgatgcaa tttgccgtcg tgcttgtaac tttggatcgt
     1021 cacaaactct ccttcttttg gaactcccat agcaaccctt ccttaagcag cattttgtgc
     1081 tttgtgtttg ggtgttcatt acaccgacat cataattatt atatcaataa aaatgagctt
     1141 tgcctagaaa ggattcctat tgtttcgatt tttctctaac tgatctacca gtttcatttg
     1201 gaccttagga aaaacgaccg aatcaaatgc atcaggcttc aaccacttcg aagttttgtt
     1261 ctcggtaaat tcttgttctg ctccctcggt tgcccgtccg taaaaaagga gaacatgcca
     1321 tttcagatga ctaaaaatat gagtaacttc tcctaaatgt cgtgtctgcc aaacaaagag
     1381 ttcctgcttt tcaaatggga gattttgctc ttcttctgct actaggtcat caaacagatc
     1441 gatttctggc tttgtttccc attctttttt caatcgctca tactcctctt gtgtcacttc
     1501 catcattgga aatgtccaca tattcgccag caatttttgg ctatctcttt cttcaaaata
     1561 atacgctcca gaatgatttt gaagagctgc actgatataa taaacgtctt ttggctttgc
     1621 tttttttgtt tttacaggaa aactcgtttg gattcctcgt ttgttagcta aacaaaaggc
     1681 ttggatcgga caagtctcac actttggaga agttggtgta cagatagctg aacctagatc
     1741 catcattgct tggttgaatt cacctggatg cttttcatca atgattttcc gcatcgcttc
     1801 atcaaagatt ttcctgctag aagcttttgc gatgtctgct tcaatgcaaa acaatctgct
     1861 gactactcgc atcacgttgc cgtcaactgc aggttctgga agtccaaatg cgatacttgc
     1921 aatcgcgcct gtcgtatatg gtcctattcc cttcaatgag ctaatttctt caggcgtttg
     1981 cggcatttcc ccatcaaatt ctgacatgat ctgtttagca gcagcttgga tgttccgggc
     2041 tcttgagtaa taaccaaggc cctcccatgc ttttaaaagc ttttcttctg gagcatttgc
     2101 tagctcttca attgtaggaa accattccat gaaacgataa aaataatcga tcactgtgtc
     2161 tactctggtt tgctgaagca tgatctcaga gatccagata cgataaggat cacgattata
     2221 gcgccaaggc aggtttcttt tttcttgttc ataccattga atgaactgat cttgaaattc
     2281 ttttgtttct ttatctgtcc atttttccca ttctttattc actttcctct tccttttccg
     2341 taatgaatcg ctggatcata tccaaatcaa atcctttttg atataagctt tgctttactt
     2401 ttagatttct ttttttaggt tcaaacctta aatttcttcg ccatagttta tctccttgtt
     2461 tgactaaata atcatattct atttcttgat ctgcttcttg cggaagatca gctatcgctt
     2521 gttggatcac ttcttgagaa aagcctttcg tcatcaatgt ttgttggatt tttctcaata
     2581 attctttttg gctttttccg tgattttttc caaacgcttt ttcggctgtc tgatgtgcat
     2641 tttgaacttg gtcttcaaat gtatattggc tgagcgcttg ttcaatgatt tcaggtttca
     2701 aacctttttg ctgcatctgc tgcgctaatt tctttggtcc tttgtcactt aagcgcatat
     2761 tcgtgcgcac ataactttcc ccatagacca aatcatcgac aaggtccaat tcttttaagc
     2821 gggctacgat tttatttcga tcttcttgaa ggatttcctt ttcttttaag tatgttcgga
     2881 tttctttttc cgatcgcaac tgataactta agtaattcat tgctaggtga aaaccgaaat
     2941 cagctccgct actcttcttg atctcttgga tagtttcttc gtctaattcc cgtcctttta
     3001 ataatcgata gcgtactaat acatcttcag atacttgtaa agcttctcct tcagataaat
     3061 ctacgcgata gaaagggcct ttgcctttac tgattctcgt aacggttaac ataaacatcc
     3121 tcctcgaaca tatattctct atcataccac agaaaatccc cgtaatcagg tattatatgt
     3181 tagaataaac cacagaaaaa agaaaaaggg agatacttat ggcagaaaca attgtcaaaa
     3241 ctggacaaac ggttcctttg aaaatcaagc gtttaggcat aaatggtgaa ggaatcggtt
     3301 atttcaaacg gttgatcata tttgttccat atgcactacc aaaagaagaa gtactagtga
     3361 aaatcacgaa agcaactcct cgctatgcag aaggagaatt aataaaagtc aaaaaaacaa
     3421 gcaaagatcg cgtagtggca ccatgtcctg tttactatga atgcggtggc tgtcaattac
     3481 aacacttagc ttatcaagct caattggatt ttaaaaagga tctgcttttg caagcattgg
     3541 aaaaattcaa accagcaggc tttcgatctt atcaactttt gccgacgatc gggatggatg
     3601 agccttggca ttatagaaac aaagcacaat ttcagttacg aaaaaataag cagactcaaa
     3661 aaattgaagc tggtttatac gaagcaaact ctcatcagtt agtccctata acagactgct
     3721 tggttcaaga acctcgaaca caaaaagtaa tgaatactgt tgttcaacta ttgaataaat
     3781 ttgatgcacc catctatgat gaacggacaa acagtggaat cttccgcaca atcatggtta
     3841 gaatcggtgt taaaactggg gagttacaag ttgtttttat tactcgcagt aaaaaattcc
     3901 cgcaaaaaaa cgcaatgatc cgtgagatca atgaacgact gcccgaagtt gtttcaatca
     3961 tgcaaaatgt ccaggcacaa aaaacgtctc tcgtgatggg ggatgaaacg atccacttat
     4021 ggggaaaaga agccattgaa gaacaaatca atgaagtgac tttcgatctt tctcctcgtg
     4081 ccttctttca actcaatcct cagcagaccg aattgcttta tggcgaagga atcaaagcat
     4141 tagatgtcca gcctaatgaa acgatcgtgg atgcttattg cggagttggg acgatcggct
     4201 taagtgtagc aaaacaagcc aaagaagtac ggggaatgga taccgttcct caagcaatcg
     4261 ctgatgcaaa aaaaaatgcg gaaagattag gatatcacaa cactcgatat gaagtcggga
     4321 cagcagagca agttctgcct aaatggttga atgaaggttt tcagcctgat ggtatcatcg
     4381 tagatccacc acgtacgggt ttggataacg cattgatcca atcattattg aaataccctc
     4441 caaaaaaact tgtttatatc tcctgtaatg tttctacact tgccagagat ctggttaatt
     4501 taagtaaaaa gttcaagatt gaatatttac agtccgtcga catgtttcct cagactgcac
     4561 gttgtgaagt tgtcgtgaag atgacgagac tcgctgacta aatgaaagag gttgtgaaaa
     4621 agtctgccct gtatcaagta ataagaacag ccaaacaaaa atagctcttc atgttttatg
     4681 aatactgtac ttgtgaccga agagttaaca ggaggacact atttatttaa aaaaagggtt
     4741 gtgacatagt ttttgtcaca gccctttttg attattattg gatattcacg tctatcactt
     4801 gataaaatgc atttgtcgta tcgctgatat tccatactgc taaaatcaca ttatatcctt
     4861 ttcgatcctg cggaagtgtg atcgtttggt tgactgtttt tggtggaaca gttgctttgt
     4921 cgtctatttt tgtgatcaat tcaaaatcac taaatttcaa aggttgatta ggattccaag
     4981 ttggtttggt aatgtagtaa tcccaagtac ttgtccgatg ttgtgctgtt aaattccaag
     5041 taatatttaa tgttcctgga ttaaccatgt ttttatgcca tctgttagat gtctgttcat
     5101 ctaaaggctc aaagccgctg actcctgcac tagcaatttt tccatcaatg aatgtatttt
     5161 tggtagcttc gatactttgc ggttcccatt gtgctcttcc gacattttga tttaagtttc
     5221 ctgctgctga tgttcctagg tatgcacggc ttcctggaga agtaatataa ccatgcgcaa
     5281 aacctggaat gctaaaaccg aacaacccta aactaactaa cccaataaac aataacgtac
     5341 taatcttttt catgtagaaa cctccttcat ttgataacca cattgtaaac gctttattgt
     5401 attaacacaa acattatgct tttataattt tatgttgaag taaaaaaggt gaaaaaagta
     5461 agatgcagat tataacagaa gtagacgaga aaagtacaaa tagaaaacat gagaatcaaa
     5521 tcatatgatc tttttcaaaa acatcattca tataaacaaa tgttcaactt tttaataaca
     5581 acaattatca atttttttct tacttcacaa actaaataca ggtcttgctg tgagccttca
     5641 tggtaaacta atattataga aaggagttag taaaatgaag atgataaaag atattcgttt
     5701 ttgggtttgt gttataggaa ttgtcatatt aggcttttta tcagggttgc ttagcggaaa
     5761 tcccggaaaa tattactatt ctttgcaact tccaccattt gcaccaccta gctggatatt
     5821 tgggccgatg tggaccttac tgtatatatt gatgggtatt tctctttatt tgttacttaa
     5881 tcacaacaac aaaaaacagc gaaacaatct tgtcggtctt tttgtgattc agttcatttt
     5941 taattttata tggtctgctc tattttttaa tttaagaaat attttcattg cagcaatcga
     6001 tattacactt ttagttattt tcttgtcagt gcttatgtat cagttgtggc tgcatcatcg
     6061 cctggcaatg tggctcatga ttccttatta tttatgggta ctattcgcaa ctctattgaa
     6121 ttattcaatt tattttttaa actaagcgct aaaaagagac gtcctgtaat ttaacacata
     6181 gaaaaagtgc cacctaagtt agattttttg tctaatttag gtggcactct ttattaacca
     6241 aactattttt ttctagtacc cacttgttgt tccatactca ttattctggt cataaccata
     6301 accattttgt tcctgtccag cgtaaggatc ataactttga gtatcttgac ttggtgtata
     6361 gtactgactt gtatcttctg tttgctgata ataggtattc ggatcttggt actgatcata
     6421 tgtgttttct gaataggaag aataatcggt gtacccttca ctattcgatc catcaccgtt
     6481 tgtcgaatcg ccatcttccg aatcatcaaa gaacatcatt gaagcattct catttttcaa
     6541 attaggtaat gtatcgcttg tttttatatt taattgtttc ttcagttcat tttggatcga
     6601 aagaagatca ttttttccta aaatttgata gtagatatca ttgatcattt gtccttcgcc
     6661 ttgcaactgg tcttgtttga ttgtttcaaa cgccggggtg tagttcgtcg ctacatcaag
     6721 catgtcatcc caatctaagt ccgtcgtcac attgttctca actgctttta aaatttttct
     6781 ataattgctt acgccatcta aacttaaaac tttattgacg attttcgtca ctacttcccg
     6841 ctgacgacgt tgtcgaccga tatcgccttc tggatcttcg tgtcgcattc gagaataggc
     6901 taaacctttt tctccattca atgtttgttc acctactggt acatggacac catccaaagt
     6961 aaagtcaatt ttattattca ctttgatgcc accgaccgca ttgatcaaat cttccatgcc
     7021 atccaaattg atcgttacat aatgatcaat aggaatatcg agcagttgct caattgaatc
     7081 catggccatt tcgacaccac caaatgcgta ggcatgattt aatttatcta ccgttccata
     7141 acctacgata ttcgtataaa tatctcggtc caaactgatg atcgttgatt ttttttgttt
     7201 cggattaact gttactacca tcatcgtatc agaacgtcct tgttccgttc ttcctaatcc
     7261 accggtatct aaacctaata gcaaaaccga aaaaggttct ttgtcatcaa tgcttactct
     7321 ctcagaacgt ttggaggata ttcgattaga gtttttagaa attttgttta ctgtttggct
     7381 cgcttctccc ataaatctga ttccgtatgc tgataatccg gcaatcagca aaacgaatac
     7441 ccctaacaag gttaatagaa ctttttgcca tcgcttcata atacacctct gtttcacatt
     7501 ttctaaaata ttgtctcata taactgttca atcagcaaat tcacttttta atcataagaa
     7561 aactttaaaa aacatttttg tgtaacgatt attatttttt aaaatttctc aaaattctta
     7621 actatcatac cataccgtga aaaagatcga aagaccgaat acacgcagtt cgcaatcgtt
     7681 ttatcttttt ttatgtattt tttccagaaa aagcgaacaa caaggtattt tccaaataat
     7741 cgtgcagtaa aatgatcgca catacctttc ccatcttttt aacagtcttt gcctaccgtt
     7801 cagattatca ttcgatttca cgacaatttc ttcctatgac tggtcctttt gttaaaccca
     7861 tcttctattc gttttatttc cagctcactg catggctctt aaaatgactc gaactagtga
     7921 ttgcattacc atcatctttt aatggataga tatgctcctc ttcaaagtaa aacgattttc
     7981 ttttaaagta atagaattga catggtaatg agtatgtata ctagtggttt cacaaatggt
     8041 cttttattca acatccaaaa actgaaattt ctttaaaatc cattactaac gaagaaaatg
     8101 ttccttgctt gataattagt taggacatgt caagtgaaca aatgcattta cttagccaaa
     8161 aaatctaaaa acttcataaa tagacaaaaa gacacgactc tctaagaagt cgtgtcttta
     8221 ttcaacaaat tattataatt tagtcacttc agcagcttga ggtccgcgtg caccttcaac
     8281 aatcgtgaat tctacttctt gaccttcttc taggctcttg aagccgtctc cttgaattgc
     8341 tgagaaatga acaaatacgt catctccgcc gtctactgta ataaacccaa atcctttttc
     8401 attgttgaac catttaaccg ttccgtgttc cataaatgta tatcctccaa aaaagttctt
     8461 gcaagtaacc ttgcaagaac attataccca atttagaaaa cgctagcaag ctttcgctct
     8521 tttattttcc aaattatttt taagactgat atgacggagg aaatggttat tttgctgctt
     8581 cgaaacgttt gtttacttca tcccaattaa taacattcca gaatgctgca atataatctg
     8641 gtcggacatt tttatatttc aaataataag catgttccca aacgtccaaa cctagaattg
     8701 gtgtctttcc ttccatcaat ggagaatctt gatttgcagt agaggtaata gctaattttc
     8761 cattttccat tacaagccaa gcccatccag aaccaaatcg tccagcggct gcttttttga
     8821 attcttcttt aaaagaagaa aaatcaccaa aagcttcatt aatcgcttct tttatttctc
     8881 ctgtaggttc gccaccagca tttggtgcca tgatttccca gaaaaatgaa tggttagcat
     8941 gtccgccacc attgttacgt acagctgtct tgatatctgt tggaatagcg tccatatcag
     9001 ataataattc ttctattgtt ttttcgccta attctgggta tttctcaatt gctgaattta
     9061 aattcgtcac ataagtattg tgatgcttat catgatgcag atgcatcgtt tcttcgtcaa
     9121 tataaggttc caatgcgtca tatgcataag gtagatctgg taatgtgtaa gtcataaaaa
     9181 aattcctcct caatgttttc ttaataatgt ctacattaca aagagtaaca aagagaagga
     9241 aattcttcaa ttgttatgcc tagtcgtctt tttcactttg acctacttct tcttcaaaaa
     9301 cctcagtttc tttaaaaata aacaacttgc tgaaaatata gttcgcaagg ataacaataa
     9361 tttgagtgat gattttcgcc acatactcat tgctgttcat aacattgatc aacaatacca
     9421 ttgcccccat gtccaatccg taagaaagga tacggtagaa gaagaactta ccgcattcaa
     9481 taagtgcatg tgtaaaattc ggtgattttg aatggaatac ccaaagtttg ttcgttacaa
     9541 aagcaaacaa tacagaacaa aaccacgaaa tagaattgga taaggccata cttaaatcaa
     9601 aatattggcg tgcgagagca aaactaacaa aatttacaat cgtagccaaa ccaccaaaaa
     9661 agagatagac tagtatctcc caatatcctt ttgcttctaa atatcctttt aactgtcgat
     9721 actgcttcat gagccaacct accttattaa aaattcttat ttatcatact ctatatttca
     9781 ataactttca tctttattta aaaaaattaa ttttttttga tcctttgcct ttgtctctgc
     9841 ttggatcgtc acatgtgtaa taccatattt taattttaat aaagaaatta tacgaggata
     9901 tatcttttca atctcgctta gtagctgatc ttcaaaatca atatgtccgt cgaaaataat
     9961 tatcttttcg ctatactgcc aaatatggac atgatgaaca tcctttaccc catcgattgc
    10021 ttcaatttct tgttccattt tttcatagtc gatagcaggt gcactttgta tcaaaatagc
    10081 ggcagctttt ttgatcacca ttatcgcttc tatcaatata tatatcgaaa tgatcagtgt
    10141 aataactgga tcgatccagt agatatcgaa aaaacggatc aaaattgctc cgataatcac
    10201 acttaccgaa gacagtgtat cacttaacaa atgcaaataa ctagatttta tattcaaact
    10261 ttcatctgct tcttgtcgta ataataatac tgagaacaaa ttcgcgagca atccaataac
    10321 tgctactgtt agcatcaaat gactatttat cttttctggt gaagtgaacc gcttgaatgc
    10381 ttcaacggtt aaaaacaagg ataatatgat caatatgaat gagttgatga aagcactcaa
    10441 tatttctaaa cgctggtagc catacgtacg ttttttattt gcttcttttc gggccatttt
    10501 ttgtgccccg taagaaaaaa taattgccaa tgtatcacta aggttatgga aagcatctga
    10561 aacaagagac aaactccctg acataatccc tccaataaat tctgataaag tgatcaccgc
    10621 attcaaaacc actactccaa atagtttttt tgatgatctc tgtggctgtg taggcactat
    10681 tccttcctcc attttattta tattggatag tctttagaca agaataacat ttttttgata
    10741 tttcaccaat cattcagctg tttccttaaa gcacttgtat ttcacttgat acttcgctag
    10801 aattgcaagt gatgacacta ctagatgagg agtacttatg acaacaaaac aattaattat
    10861 cagttccttt atacgattca aagacctaaa ggaggcgaga aatgtcccat tttggaaagc
    10921 cattgtctat cttttagttt tgagtataat tatggctttg ccaatctctt ttcaagtttt
    10981 tcaagtctta aacaacattc aattggatgg gcaaaagatt gctactcgca ttcctgattt
    11041 tacaatccaa aacgggcagc ttcaaaccga agaaaaagaa ggattcattt accaaacaaa
    11101 ctccattatc ttcacatttg atccagaggg aaaacgaaca gaacaggaca tctctactga
    11161 tttgatgggg aattttgtta gtgtcgggat gttaaaaaac aaacttatca tagctcttcc
    11221 taatacaggc acgacttctg ctttgttgaa caataaccaa ttagaacttc cctacaccaa
    11281 tgaatcttta aaaaacttaa caggtaagca gctccgttct tttttaagtg aagcatctat
    11341 accactttgg ataaaagtcc tgacatttat attttctatt tatcctagtt ttctgaatct
    11401 gctgatcact ttgctattta ccaacattgc tgcttttctc tatgcacgct ttcgactaac
    11461 aaaagctact tttcttgatt gtttaaaaac attgatctat tcaatttcat tgcctactat
    11521 aattgcaaca atcttgatgg tctttcttcc ttcttttgac actagcgcct tcattgcttt
    11581 tgcaggaatc tttatttttg ctcaagctgt caaaggatgg g
//
LOCUS       DASJON010000089        11592 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJON010000089 DASJON010000000
VERSION     DASJON010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.089"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..971
                     /locus_tag="U3989_001707"
     CDS             108..971
                     /locus_tag="U3989_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1715159.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1100..2722)
                     /locus_tag="U3989_001708"
     CDS             complement(1100..2722)
                     /locus_tag="U3989_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1715160.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2971..3492
                     /locus_tag="U3989_001709"
     CDS             2971..3492
                     /locus_tag="U3989_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1715161.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="U3989_001710"
     CDS             complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="U3989_001710"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN1715162.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5573..6391
                     /locus_tag="U3989_001711"
     CDS             5573..6391
                     /locus_tag="U3989_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN1715163.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6494..6691)
                     /locus_tag="U3989_001712"
     CDS             complement(6494..6691)
                     /locus_tag="U3989_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715164.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6860..7438
                     /locus_tag="U3989_001713"
     CDS             6860..7438
                     /locus_tag="U3989_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1715165.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7533..7670
                     /locus_tag="U3989_001714"
     CDS             7533..7670
                     /locus_tag="U3989_001714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715166.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8483..8986)
                     /locus_tag="U3989_001715"
     CDS             complement(8483..8986)
                     /locus_tag="U3989_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1715167.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9345..10370
                     /locus_tag="U3989_001716"
     CDS             9345..10370
                     /locus_tag="U3989_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1715168.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10693..11571
                     /locus_tag="U3989_001717"
     CDS             10693..11571
                     /locus_tag="U3989_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN1715169.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3591 a         1973 c         2256 g         3772 t
ORIGIN      
        1 attttgatta gaacttgcga aaataaaccg gttaaagtgt gtctagcgca agagatttgc
       61 tataatagac gataagggta aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg
      121 taacagattc ttgttgtgat ctgccatacc atgtattagc aaaagaaaaa gttgattttt
      181 tatcaatgaa tctccaattg aatgatcaag agttagtaga tgacctcgga aagacattca
      241 attatgaccg ctacatgaat cagctgaaaa atggtgcgat gcctacgaca tcacaaatca
      301 atgttggccg atatttagaa tttttccgtt cttatgcgga aaaacagatt cctgttttat
      361 atgtggcttt ttccagtgga ttaagcggct cttacagtag tgcgcttcaa gcggttgaat
      421 tgctgaagga agaatatgaa gaccctgaga tctatgtttt tgatacaaaa gctgcaagta
      481 ttggggaagg gcttttagta ttggaagctg taagattgaa aaaagaaggt aaaagcatcg
      541 aagaaaccct agagtggctg gaaagtaatt atttgaaagt ccattcatgg gtaacagtag
      601 acgatctgaa atatctagaa cgaggcggta ggatttccaa agcaagtgct actcttggca
      661 gtctattgag tgtaaagcct attattacgg tggataaaga aggaaggctg acgaatgttg
      721 acaaagttcg aggaagaaac aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa
      781 tcgttgcacc acttgatcaa atcgtttatg tggcttacgc tggggatcta gagagcgcgg
      841 agaaggcaaa acggcttttg gaagagcaga taaagatgaa agatgtgaag ctttatccct
      901 taggaccaac gatcgccagt cacacaggct atggctgtat cgcgattttt tcgatgggaa
      961 tcagtcgata aaagcagtaa ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa
     1021 acagcgactc ttaaaagata gatgagcatc ctatctctta gaggtcgctg tttttttatt
     1081 ttttaagcta aataggccat tatgcccata actcgttcaa ttgtttttga atatctgcat
     1141 tttccaaaaa ttcatcatat gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg
     1201 caatgatgcg gtttgccaat gtttggataa attgatggtc atgtgacgca aataagattg
     1261 agccagtaaa tgccatcaag ccgtcattca atgctgtgat tgattctaag tctaaatggt
     1321 tcgttggatc atctaataca agtacgttag ctttcgaaag catcattttt gaaagcatgc
     1381 aacggacttt ctctccacca gataaaacat tcactggttt caatacttct tcaccagaaa
     1441 acaacattct tcctaagaag cttcttaaga aggtgttatc atcttcttct ttacttgcat
     1501 attgacgcaa ccagtctaaa atcgttagat cagtatcgaa ttctttcgtt gtatcctttg
     1561 gtaaataaga attacttgtt gtcacacccc aacgtactgt tccagtatct ggcgtaattt
     1621 cacccatgat aactttgaat agcgttgttg ttgttatatc attttctgcg acgaaagcga
     1681 ccttgtcttc tttgctcaaa ttaaaagata tatcatctaa aattttcttt ccatcaatcg
     1741 ttacacttac attttctacc tgcaataagt cgtttccaat ctcacgttca ggtttgaatc
     1801 caacgaatgg ataacgtcta gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca
     1861 tttttttacg tgaagttgct tgttttgatt ttgatgcatt cgcactaaag cgggcaataa
     1921 attcttggag ttctttgatt tgttcttctt ttttggcatt tgcgtttgct tgaagtttag
     1981 ccgctagttg actagattcc aaccaaaaat cataatttcc tacataaagt ttgattttac
     2041 caaaatctag atccgccata tgtgtacata ctttgttcaa aaagtgacgg tcatgggata
     2101 caacgatcac agtattttca aaattgatca aaaattcttc cagccaattg atcgattgga
     2161 tatccagacc gtttgttggt tcgtctagca aaagaacatc aggtttacca aatagtgttt
     2221 gtgctaataa cacttttacc ttttgtccac cagttaattc actcatttgc tgatggtgca
     2281 attcttcagg gatgttcaga ccttgcagta aaacagctgc ttctggttca gcttcccatc
     2341 cattaagctc agcaaattct ccttctaatt cagctgctcg aataccatct tcgtcagtga
     2401 aatcctcttt catgtaaatc gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc
     2461 ccatgatgac tgtttcaagt acagtatagt cttcatagtc gtaatggttc tgtttcaata
     2521 cggccatccg ttcatctggt cccatagtaa cggaacctgt ggaaggctgc agatcccctg
     2581 ataacacttt aaggaacgtt gatttacctg caccatttgc accaatcaag ccatagcagt
     2641 ttccgggggt aaatttaata ttcacatcat caaatagttt gcggtctgaa aagtgcaaac
     2701 tcacatcatt tacagtaatc aatttttttc ctcactttta tcattattag ttttgacaag
     2761 tcttatagaa ttatataggg gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt
     2821 ttcacgtgaa acagaatgaa aaaaataaaa ataaaagcgg agcgaaaata gctactatag
     2881 gatttattag tttgcaacag cataattttt gacgttattt ataagaggga gtactataaa
     2941 actgtaaaag aaatgaaaga aggattgaaa atggatgaaa aaagaaccat cgactggggc
     3001 tcattgattt tgggaatttt atttgtactt gtctcattgt tatcatttca agaccctgtc
     3061 ggaaatttag ttgcaattgt agtagtcttt gcgatttttg cctttattaa agggatattt
     3121 gaactgtttg tacggaacag attaaaagag ctaacaggat ataaaggaaa gatgccctta
     3181 ataatcggga tcatcgatat tctagttggc gtctttttct tattcaatat tggtgcagga
     3241 gtcgcagcat tgccttttgt ttttgcagta tggtttattg ctgattcagt tttagcgtta
     3301 tttactgctg acttggcaag aggagtaagt gaaggatatt attggtttac gatcatcgtc
     3361 aatattttag gaattttatt aggaattttc ttattgttta atccaatttc ttctgcgttg
     3421 acattaagct ttttagttgg attctatttt atgttatttg gtatcacaca tatcgtctat
     3481 gcatttagat aagtaaaatc aaacaaataa aaaagccggc ttccgattac agttttactg
     3541 ttgtaattgg aagccggctt ttttatcatg gacaaatatg cacgaggtga tttttaggtg
     3601 tcgagacaaa tttatgcaaa aatgtgagaa gctatataca caaattttat cttaatactc
     3661 gaagcatagc gcagttgtca caacctcttt tcatataatt cattcaatac tggcaaatca
     3721 tggacttcat atttttcttt gaatgcttgg atttcttctt cagaatattt tcttggatct
     3781 aaatgcagat cttctactgg caatgggcgc ttgttattga tcttcacatc gattactaca
     3841 ggacgatctg attttgcagc tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact
     3901 gtaaagctat ttgccccgag cgcttctcca actttaccga aatctgcatc ttctaaaaat
     3961 acaccaaatt tcttttgttc cgtatcttct tgttctgctt cgatgaaacc aaaagaatca
     4021 ttcgaaaaga caacattaat aattggaagc tgatatttga cttgtgtaat gatatcttgc
     4081 atgaccatag caaacccgcc atcaccactt aaggtaaata cttgtttttc tgggtagctt
     4141 agttgtgcgg caattcctcc tggcacacca ttacccatag ttgcgaacca tccagatgtc
     4201 gtatgttttt gtttaccgtt catattcaat aaacgaatag aatggatagt cgtgttaccg
     4261 acatctgtga cgaaaatcgc atcgtcttca gcaatacgat tgatttcttt atagacagcc
     4321 tctggacgaa tcggttcttc ctctcgatct tcaaaagatt tcaaccaatt tacccatttt
     4381 tctttatttt cttgatttgc taggtaccag ctatcagcag ggcgagcatc ccctaactcg
     4441 acaagtcttt gcaaagctgt gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt
     4501 cgtccaaatt ttgacgcatc gatatcgatt tggataaatt cagcagttgg attaaaaaat
     4561 gcacgtccaa atgggaagtc gctaccgaca aataagatca gatcggcttc tgccaatgct
     4621 tcatttccgg gtttagtagc gacacgtcca gcaaaaccaa gaaagttttc ataatcatca
     4681 ggaataattc cttttgctag aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat
     4741 tctttgattt gcggaaatcc gtttcgcgtt ccttgaccaa cataaagaac tggttttttt
     4801 gctttttcaa gataaggcaa agcagctagc aaatcttttt cttctggtaa tatcacactt
     4861 gttttgtgag tgtgagcggt ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca
     4921 acagggatag tcacgatagc cacgccttta tgctcatagg cggctttaat ggcttcatct
     4981 actacatgcg gaagactttg tggtgtcatg actgtacgat tgtatacgct aacatctgcg
     5041 aaaataggat tttcatttag ttcctggaaa gagttatagt tcatggatgt agaagcaact
     5101 tgtcctaaga acgctactag aggcacatgg tccatctgtg catcatacaa accattgatc
     5161 aaatgtgtcg caccaggtcc tgcagagccg aagacagcgc cgactttccc cgttaatttt
     5221 gcatctgcag cggctgcgag tgcgccgact tcttcatgac gaacttgaat gtagttgatt
     5281 tcggtttttt cttgatatag cgcatccata attgaattga aagatcctcc gggaatgccg
     5341 taaatatggt cgatacccca gctttcaaga acttttacca ttgcgattcc tgcattgatt
     5401 tttgacatgg tgtttcactc ctttagcaat gatgatacat aaattttttg tatctagagt
     5461 atagaacttt ttatcgaaaa tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt
     5521 tttgggaata tgctagagta gtacatagat agattcaaga gaggggaatg gcatggttga
     5581 atggctattg agacttgaac cttggcagca agcacttgct gggacagctt ttacttattt
     5641 tatgacagct cttggtgccg ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct
     5701 aaacttgatg ttaggatttg cttctggtgt catgattgct gcaagttttt ggtctttgtt
     5761 agatccggct attacaaaag cagaagaaaa tggagatatt gcttggctag ttgtaagtat
     5821 tgggtttggt ttaggcggtg tgtttttata tatggcagat aaaacactgc cccatatgca
     5881 ttttggacca caacatgaag cagaaggatt gcctacccat ttaaaacgga cgatcttgct
     5941 tgttttttct attacattgc ataacattcc tgaaggttta gccgtggggg tagcattcgg
     6001 tgcagcagca actgcggata atccgacagc cgctatatta gccgctgttt cggtggcgct
     6061 aggtattggt atccaaaact tcccagaagg agcagctgta tcaatcccgt tgcgacaaga
     6121 aggtctgagt cgtaaaaagg cctttgttta cggacaagct tctggaatcg ttgaaccaat
     6181 tgctggagtg attggagcgc tactagtaac gaaagtggag ctgctgcttc cttatgcact
     6241 tgcttttgcc gcaggagcaa tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca
     6301 gcagacattg tccagcaaac atcattttgc agtttttggc gtcatgagtg gatttatcat
     6361 tatgatgatt ttggatgtgg cattaggata aataaaaaaa atatatatga tatatcaaat
     6421 agggccgcga acttatatcg cgaccctatt tttttagtaa gttatatcat aatgaagtga
     6481 atggtatcat acattaataa tagttttttc tatgtgtatt tcctttgatc cagcgaacaa
     6541 gccagatgat aagtgcgatt gggattagaa ctttgaccgc aaaccataaa agcccgctta
     6601 ggacactgac aaaaagactg atgacgaagc tagctgcaat tagcactaaa agaaccatga
     6661 agatatttaa gaacatattt ttttgacgca tacaaaaccc tcctcttgtt cattctttac
     6721 tatagaaaaa gtataacagg gattgtttaa aaacacctcc aactgaagac tgatttttac
     6781 ttcatctact taaatgatca aacaattgct ttttctctgg tttatcatct aaaatagagc
     6841 ggaaaaggag gagaatatca tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat
     6901 cgcgattatc atgactattt tagttttaga acttcatgaa cctaacggag acacgtggga
     6961 ggcgctcgct gcattaggtt accgattttt tatctatatt gtcagtttta tcattttagc
     7021 gatttattgg aacaatcatc atcatttatt tcaagttgta cataagataa atggacgggt
     7081 cttatgggcg aacagtttct ttgtttttgc gctttcgctt tttccgtttg caacttcatg
     7141 gatcagtgag tacatcaata gcctggctcc agaaatgact tatggttttg ttaccttgac
     7201 cgctaatatt gcgtattata tcttggcaaa agaacttgtc aaagcaaata aaggccacga
     7261 gaaagcattg aaggcgatgt tgaataatta caagaaatcg tatctatcga ttggtttgaa
     7321 tgtgttagga attgtcttag ggtatttagt agcgccagta gccgtgctga tcgcaaacat
     7381 cgtgattcta gctgcttgga tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa
     7441 tgatccgtgt ttaaaattta agagaaaaat atagaaaaaa taaataaacg atcaaatatc
     7501 cgagtgatac tagtattcaa agagggatgc agatggatca aaaaagaatt catgtaggac
     7561 tgattactag ttttgtcacg atgcttattt atttggatca aggtcatttg acttggacag
     7621 tagttggtgt cgtaggattt ttgacattat tcacagcaat aaaaaaataa acatctttat
     7681 gtaatctgat gttctatcta tttaagaaaa gcatgtttac agcagtatga ttctgttgta
     7741 aacgtgtttt tatttgtgga aaatttaatt tttagaaaag tgtatcgaaa aatagatagt
     7801 tagtttttcc acagtggata acttcgtaag agataaaaat aaaaaattag tcaaagaagg
     7861 gcgtatagat ttcttttgat aggagatata caacagaaat gaataaatat taacgtgttt
     7921 tcaaacagta taaaacggtg tggtttttgg aataaaaaaa taacatatct cattttacac
     7981 ttttgttatt ttatacttaa aaaataacga cacagaaaat accgtgtctc agatttctct
     8041 actacttcta aaaactactg tttttttcct ttgaaatgcc ttcaaaaagt aggatcaggc
     8101 agaatatact ggcagaatca aggagaaggg agcctaaata aggagaaaaa ccatagaaaa
     8161 tataaagtaa ttacttataa aacaagcttt ttatagaaaa ataatatacc tgagtagggt
     8221 ataaattaag acggaattcc tttctagatt gagatattga ctaggaagaa tgttaagatt
     8281 ttaagccttt aagctaaaaa aaaacccttt ttgttaaata aggggaatat agcctgtcag
     8341 tttcaaactg caagtcgtcc tgctgcaata atatagtaga ttagagatga aaatagattt
     8401 ttttaaacca atgactgaat aaaaagaccg tcatagatac acattggaat gtgtctatta
     8461 cggtcttttt ttacgaacga ttttattgaa gacggacagt tcgggtcaaa aataaaaaag
     8521 ctacgatcag aaataagaat aaaatgtatc ccaaaataat ggctagacgg tctaacttat
     8581 ttgtcgatag atgtttgttt agaagttgat aggcccataa acctatcagt cctcctgttg
     8641 tattagttat caaatcagtg atgtccgcta cgccaatacc gaataggaat tgaatcaatt
     8701 ctgccaagaa gcttacaagg aaaataatcc ctaatttttt agaaaaagaa agtctcttga
     8761 aattgacatt caaaagcacc ccaaaaggaa aaaagacaat caagttataa gtggtctcac
     8821 gtaataccgt cgtattttca aaagaaaaag tagaaaaagg gacgagattg acattgctgt
     8881 aagaataagt gagaaattgt ggaagattaa atgatagctt gaataaaatc aaccataata
     8941 gaaagaataa ataaatccct aataaaaatt ttgatacata ccgcatagac atctcctcca
     9001 tttaaaaagt ataccatcga aaaccgctga aaaatcataa aaaagtatga agtttaagga
     9061 ccaacgtagt tatcgtacga aaattagaag cttttttttg ttgggaatcc ggtttcttta
     9121 acaaagctaa gatagcgagt tgcttggaaa agttgctatc ttttttctgt caaaacgact
     9181 cggaatgttc gttttttgtt ctaatttctc caagttgttt actttttgat actaagttta
     9241 tgaaaaatca agaaatagtt gttatactag gtaatgctga caaacgggat tggcatttca
     9301 tcttggttca gctttttttc caattactag gaaggaggga tagattgttc aagacttatt
     9361 ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat gaatctgatg ctacttatct
     9421 ttattgtatt atggttaggt tacataaatc atgatttttt gatttcaagt ttttcatcat
     9481 tagggatctt tacttttttc tattatcaaa acataccgat gaaatatttg ctgaaacgta
     9541 tgtttctgat cggttgcgga ctgttggttg ctttcgtttt ggggatctta tcgacttatg
     9601 ttttatggct tgaaccattt gccgtagctt tggttgcttt ttccagcaga ttcgtgttgc
     9661 gattattcca tatttctaaa ccaggtggct tgttttttgc gatgctttct gccatgggaa
     9721 cgagcatgca gctgccaatc gctcaattac cgatcgtctc actttatttt tttatgggtg
     9781 tcgtttttgc cctgattgcg gcagtgatta caaaattact ggattcacgg cctgagcaga
     9841 caattgaaaa agcaacttta aaagaaagat ttcacgaaga gcctttagtg atcattgatt
     9901 ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt cagtcacggc ttgaatctgc
     9961 ataatcctta ttggttgacc ttgtcatgtg catcgatttt gctagcagaa aacttggatg
    10021 cgatgaagca tcgacaagtc caatatctga ttggatcgat gggtgggtta tgtgtgtcgg
    10081 cattcttgtc atttgtgccg tttacgcagt tgcagaccat ttttctgatc acttttctat
    10141 atggaatcgc acaatttcta gttgctagaa attatgcagt tgctaatatt tttcttaatc
    10201 cgatggcgtt gatgctatct acacttattc gtggagcata cttgatttct ttgatcgaat
    10261 atcgtttttt agggatcgtc ataggaagtt tcatcggttt aggtgttgcg tgggtaatga
    10321 cggttggttt gcaacattat ttaacggttg ttcgtcaaaa attagaatag aaggacaaat
    10381 atatgtgcca aaagaggtta tgacaagatt tttgtcacaa cctctaattc aaggtgttca
    10441 aaagccagat tctcagcatt aagtcaaatt cacacaaaaa tgtggaaagg aactttcaaa
    10501 aaaattttat atttattcta tcaaaaaatt ttgtttatga tggtaactga ataaactttc
    10561 ctgagatggt caatgctttc cttgaaagtc ttacagatat tctttcttca tagaaagttt
    10621 aagttttata gttagtgtac aataaaagta tgtttatacc atatggtgtc cggataatca
    10681 gtcatttaaa caatgaggtt caaagatatg aaaaggggaa atttgatgaa gaaaataagc
    10741 aatgaaatcc aaattctggc agaaacttta actcttccca aagtcgttat agagcaaatg
    10801 attgtacaag caaaaaacat ccttgatgag gtgtttgagc aatatagtca tgaactaatg
    10861 gatcctaata aagcaaaggc gactgcaaag aaaatagaca aagtctatga agaggaacct
    10921 tttgcagcat tagctattta tctatatgca gcttctcaaa gttggaaaaa aatttatcaa
    10981 ccattgaaga ttccaagaac tatctatttg gcaacaatga atgcttttac tagatttatt
    11041 catgaacatt tccaagtaac aggaagctat cgctttgacc gtggtttttg gatttggcat
    11101 tacactagtg gcttgatctt tcggattggt gaattggagt acgaaatgac atattttccg
    11161 aaagggtaca cggatctaag attagaagga aagcagtgcc tttctcttca tattccttcc
    11221 gatgcagatt tatctcagaa taagataaga gatagttacg aaagtgccat tcgttttttt
    11281 gaactttatt ttcctgatta ccattaccaa gtgatgtaca cagatacttg gttactatca
    11341 cccaatctta ctaaatggtt gaaaaaagag tcaaaaatct gtctttttgc agccgattat
    11401 cgattattat cagttgatga acaggacgat agtggagtcc cttggatctt tggacgagta
    11461 gatgcccaaa tacatgatta tccagaaagt acgagtttac agcgacaagc aaaagagcaa
    11521 ttgcttgcag gcgagcacat tggctctggc ttaggtattc ttacttcata agtttagtac
    11581 ttcgaaatag tt
//
LOCUS       DASJON010000090        11337 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJON010000090 DASJON010000000
VERSION     DASJON010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.090"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(364..1482)
                     /locus_tag="U3989_001718"
     CDS             complement(364..1482)
                     /locus_tag="U3989_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715170.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1953..3260
                     /locus_tag="U3989_001719"
     CDS             1953..3260
                     /locus_tag="U3989_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1715171.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="U3989_001720"
     CDS             3379..4578
                     /locus_tag="U3989_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1715172.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="U3989_001721"
     CDS             complement(4605..5873)
                     /locus_tag="U3989_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN1715173.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6036..6682)
                     /locus_tag="U3989_001722"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="U3989_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="U3989_001723"
     CDS             complement(7090..7593)
                     /locus_tag="U3989_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1715174.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="U3989_001724"
     CDS             complement(7716..8480)
                     /locus_tag="U3989_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN1715175.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="U3989_001725"
     CDS             8588..8863
                     /locus_tag="U3989_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN1715176.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="U3989_001726"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="U3989_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1715177.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10630)
                     /locus_tag="U3989_001727"
     CDS             complement(10430..10630)
                     /locus_tag="U3989_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715178.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3401 a         2387 c         2002 g         3547 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaata
//
LOCUS       DASJON010000091        11203 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJON010000091 DASJON010000000
VERSION     DASJON010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.091"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..90)
                     /locus_tag="U3989_001728"
     CDS             complement(<1..90)
                     /locus_tag="U3989_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1715179.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSE"
     gene            400..2031
                     /locus_tag="U3989_001729"
     CDS             400..2031
                     /locus_tag="U3989_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1715180.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            2034..3113
                     /gene="uxuA"
                     /locus_tag="U3989_001730"
     CDS             2034..3113
                     /gene="uxuA"
                     /locus_tag="U3989_001730"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1715181.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            3187..4614
                     /gene="uxaC"
                     /locus_tag="U3989_001731"
     CDS             3187..4614
                     /gene="uxaC"
                     /locus_tag="U3989_001731"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1715182.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4698..5345
                     /locus_tag="U3989_001732"
     CDS             4698..5345
                     /locus_tag="U3989_001732"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEN1715183.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5465..6841
                     /locus_tag="U3989_001733"
     CDS             5465..6841
                     /locus_tag="U3989_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1715184.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6851..7645
                     /locus_tag="U3989_001734"
     CDS             6851..7645
                     /locus_tag="U3989_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1715185.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            7658..9424
                     /locus_tag="U3989_001735"
     CDS             7658..9424
                     /locus_tag="U3989_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN1715186.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            9447..>11203
                     /gene="uidA"
                     /locus_tag="U3989_001736"
     CDS             9447..>11203
                     /gene="uidA"
                     /locus_tag="U3989_001736"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN1715187.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIA"
BASE COUNT         3933 a         1434 c         2278 g         3558 t
ORIGIN      
        1 ttctgatact ttttttccag gttctaagtc agcatagata atttgtcggc gaatagattg
       61 gtaagcttga ttttggagac tagttttaat tgtcatacac aatgctcctt aacttttatt
      121 ttaactgata ttttaattct gttcatgcca tataatatca aattttgaag taatacacta
      181 gttataaaaa taaaagaata catgaaaaaa agaaaaaata agtttcgata aaatcagtcc
      241 aaaaagaagg agtattttat ttttctgaat aaaaaataca atcattctga tatggtagaa
      301 aaaagcaagt ttattccctt tttatattga caacggttac aatgggtttt aaaatataaa
      361 atgtatcaaa actaacatat cagtttgagg aggttaatta tggtttcgat taaaaattgg
      421 caaaattcta tcgaagaatt caatgaaatg cagattctac tgccgtgttt tgatgtcaaa
      481 gaagttaagc aagaaggcca gcaacatcca gtatggcttc attttggtgg aggaaatctt
      541 tatcgaggat ttcatgcaga aattgctcaa tgcttagcag atcaaaatga attagaaaca
      601 ggagtagttg tgtgtgaaac atttgatgaa gaagtagtgg aaaaagctta tcatgactat
      661 aacaatgaca ttttggaaat tgttatgagt ggagatggtg aactcaaaaa aagacttttg
      721 gcagtaacgg ctgatagttt atattgcaat ccgaagaata tagcggacta tcaaagagtg
      781 gttaaatatt ttgaagatcc taaattacag tttacaacgt ttactattac tgaaaaaggt
      841 tacagtttaa aagattctaa agggcaattc ttttcggtag tcaaagctga tatcaaatct
      901 ggacctagca atcctcaaca tacgatgagt attgtaacat ccttgttata tagcagattt
      961 aatgctggag agttgccgat tgcgatggtt tctactgata atttctcaca gaatggtcaa
     1021 cgttttcaag atagtatttt tacgattgca gaagcttggc aaaaagaagg atatgtttct
     1081 gaaaaattta ttgcttattt aaaggataaa gaaaaagtga cttttccttg gagtatgatt
     1141 gatcgaatta cgccaaatcc ttcagagaat gtaaaggaag atctattgat gatggggctc
     1201 gaggatttag atatcattca cacaaaaaaa cataccaata ttgctgcttt cactaatact
     1261 gaggtagttc attatttagt tattgaagat agttttccaa atggccggcc taaattggaa
     1321 aaggctggtg tgattatgac agatagagag acggttgata aagcagacat catgaaggtt
     1381 acgacaagct tgaatccact tcatactgct ttggcagtaa caggttgtct gctaggatat
     1441 caatcaattt cagcagagat gcaggatgat gatttggttg gtttaatcaa aggaataggt
     1501 tatttagaag gtttaccagt agttgaaaat ccaggcatta ttgatccaaa acaatttatt
     1561 gatgaagtaa ttaataaacg tttgcctaat ccctatattc cagatacacc acaacgaatt
     1621 gcaacagata cttctcaaaa agtagcgatt cgctatggtg aaacaattaa aaagtatgtc
     1681 gaaaaagacg gaaatgcaga aaatctaaca tttattccta tcgctattgc tgcttggtta
     1741 cgttatttac tggcagtcga tgatcaaggg gaaaattttg tgccaagtcc tgatccgtta
     1801 ttggtagagt tgcaggataa attatcagta ttgaaattag gtgaacaaga tccttcaaag
     1861 attcattgtg cggtacagga aattttagaa aataaagaaa tctttggttt agatttatat
     1921 caagttgggg taggaaagaa aactgaaaaa atcttttgcg aaatgctagt aggtcctcaa
     1981 gcagttcgta aaacaatcaa taaatatata acagaagttg gaggaaagta actatggaaa
     2041 tgacttttag atggtatggg gcaaatgaag aaaaaatctc actacaagaa attcgtcaga
     2101 tcccagcaat gaaaggaatt gttggaacat taatggatat accagccgga gaggtttggc
     2161 cgagagaacg aattaaatct ttaaaagatg aagtcgaagc caatggactt accttgaaag
     2221 ttattgagag tgttaatgtt catgatgata tcaaaattgg tttaccaaca cgagatcaat
     2281 acattgagaa ttataaacag acgattcgca atttggcaga atttgggatt gaggttatct
     2341 gttataattt tatgccagta tttgattggg taaaatcgga tttggattat cgtttagatg
     2401 atgggtcttc tacgttagcc tatatttcaa aaaatatccc tactgatccg gaggaaatta
     2461 ttagagaagt agaaaattct actaataatt ttgaattgcc gggttgggaa cctcatcggt
     2521 tatcacaaat taagcaatta ttagaagcct ataaagaagt tgatgaagag aaattacgag
     2581 agaactttgc atattttctc aaagcaatta tcccgacatg tgaagaagtt gggattaaaa
     2641 tggcagttca cccagatgat ccaccatatt ccattttcgg attacctcgt attgttaaaa
     2701 atcgtgacga tttagattgg ttgtgtaatg tagtggattc tccttctaat ggtattacat
     2761 tatgtactgg aagtattgca gaacgaccag ataatgatgt ctatgaaatt ctatctgaat
     2821 tttgtcaacg ggatcggatt ccttttgcac atgttcgtaa tatcaaatat ttaagtgaca
     2881 atggactcga tttttacgaa gcgccgcata aatcagaata tggttctcta gatatgtata
     2941 agatattaaa aacaatgtac gataatggat ttaatggcta tattcgtcca gatcatggtc
     3001 ggatgatttg gggagaaacc ggtagacctg gttatggatt atatgaccga gctctaggtg
     3061 cagtttattt aaatggtatt tgggaagcgc ttgaaaaggg ggcgagtaaa taattatata
     3121 agggagttta tttagaggaa aaattttctc ttctaaaaac taacggaagg aaaggagtgt
     3181 ttaaatatgt ttttagataa gaacttttta ttagagaatg aaatagctaa aagacttttt
     3241 cataaatttg caaaacacca accaattatc gactatcatt gtcatttaga cccagcatat
     3301 ttgctagaaa ataagcaata caagaatttg actcaggtat ggttaaatga taaaggagct
     3361 ggagatcatt ataaatggcg attgatgcga gctaatggta tagaagaaga gttgatcagt
     3421 ggtccaggtg atgattatga aaaattttta gcttttgtaa aaacagtgga gaaggcgcca
     3481 ggtaatccta tttttgaatg gtctcatttg gaattacaac gttactttga tattgatttg
     3541 acaatttctg agaagaatgc accagaaatt tggcgacgtg ctaacgaaaa aattcaaacg
     3601 gattcctatc gcccgaaaga attgttggag atgatggatg tgaaagccat ctgtacaacg
     3661 gatgatccta cagatgactt gtggtatcat actgagcttt ctaaacagga taattctttc
     3721 aaggtattac ctactttccg tccagataag gtctggagta tcaaatcatc aggcttttcg
     3781 aactatattt taaaacttag tcaaacagcg gctataccaa tcaaagactt taatgggttg
     3841 aagcaagcat tgagccaacg agtggatcat tttcatcaaa ttggctgtcg attagcagat
     3901 cacggtttga atacttattt ttatgaaata gcagatgaaa aaatactcga caatctgtta
     3961 ataaaagctt tagcaggaaa aacggatttc catttatcag aaattgcaca atttgttaca
     4021 ggtattcaat tacatttgat gtctgagtat acaagatatg gttggacgat gcaaatgcat
     4081 ttaaatgctt taagaaataa ttcaagtata ttaaaggaac aaattggagt agatgtcggt
     4141 ggtgattcga tgggggatca agctagtttg acacaaaatg ttgtagcatt gttcgatacc
     4201 gctgaaaaga gtgggtgctt accaaaaaca attttatatt cattaaatcc aactgattgg
     4261 cttccactag caacagcaat gcaaagcttc caagggggta tgattcaaaa aatccaattg
     4321 ggatgtgctt ggtggttcaa tgatacagct gaaggaatgc gtcaacagtt gactacgatg
     4381 gcacaacaaa gtttgttgtc gaattttaca ggtatgttga ctgattctcg aagttttcta
     4441 agctatcctc gccatgagta ttttcgtcga gtattatgtc aattactaag cgagtgggtt
     4501 aaagcaggcc gaattccaga tgatgaaatg tatcttagtc agatagtgga gaatatttgt
     4561 tatcgtaatg cgtataatta ttttaatttt tttgaaatcg gaagtagaaa ataatacttg
     4621 aaataaaagc ttaaatttat tctttgtaag ttagtacagc tagacaaagt atgtatccat
     4681 aaaggaagga gatagatatg aaaaaagtag aaattttaag taagttagaa gcggctggag
     4741 tcattgcggt tgtacgtggg aaaaataagg atgaggccat taaagcaata aattcaatta
     4801 ttgcgggtgg aattaaaggg attgaactaa cttttacagt tcctaatgct gaagaaataa
     4861 tcaaagaaat tgctaaaaaa tatgctgatc aaaaagatgt aataatcgga gccggtacgg
     4921 ttttagatgc gtttactgct cgtatcgcaa ttatggctgg ggcagaatat atcgttagtc
     4981 catcttttga tcgacaaaca gctgagattt gtaatttata tcaaattcct tatttaccag
     5041 gttgtatgac aattactgag ataaaggagg cacttactgc tggcgtagat atcattaaac
     5101 tattcccagg ttctgctttt ggtccatcca ttgtaaaagc ctatcaagct ccattaccac
     5161 acgtaaatat aatgcctact ggcggagtaa gtatagagaa cttagaagat tggtttgaag
     5221 taggagttgt tgcagttggt gtaggtggga atttattggc ttctgttgaa aaaggtgatt
     5281 ttgaagaagt aagtcgagta gctagtaaat atatagataa gttttatgag ataaagaatt
     5341 attaggagaa aaaacaaata atactctgaa atgtaaaaaa taaatactga ctctttacgc
     5401 ttcagagtat agtacgtact ttcatcagaa tgaaaacgct taacaacaga agggagagaa
     5461 ttgtatggaa agaaaatttg gtataagaga caaaatagga tatatgtttg gagattttgg
     5521 aaacgatttt actttcattt ttgctagttc ttttttaatg atcttttata caaaagtatt
     5581 ggggataagc ggagctgccg ttggaacatt atttttagta gcaagattta tcgatgcagt
     5641 tactgatgta actatggggc gtattgttga tagatcgaaa gcaactaagc agggtaagtt
     5701 taaaccgtgg attttaagaa tgagtggtcc agtcgcttta gcaagttttt taatgtatca
     5761 aacttctatg atttcagctt ctatgagcgt taaggtagta tacatgtatg ttacgtatat
     5821 attatggggg agtatttttt acacatctat aaatattcct tatggatcaa tggcatcagc
     5881 tattacttct attacagaag aaagaacaat gctatcgaca ttccgaaatg ttggagctac
     5941 tttagcaagt ttggtcattg gtgtattaac accattattt atttatacaa gagatgattc
     6001 aggcgctcaa atagttcgtg gtggatcaac ttttacaatt gttgcgggaa tattttcgat
     6061 atgtgcactt atttgttatt taatatgtta taaactaact actgaacgag taagaattga
     6121 ccaatctcaa tacaaagttt cattgttaaa atctttaaaa gggttagtta aaaatagggc
     6181 attactttct ataattggtg cggctatttt cttactcctt gctcaattat taattatgag
     6241 tatgaataat tatgtatttc ctgattacta taatgatgca cagggaatag ttttaatgaa
     6301 ttttatcaat cctattttgg ttttaatagt tgtttcgcct ttaactttgt ttttaagtaa
     6361 aaaatatgga aaaaaagaac tggcatcagt aggaatgttc ttttcagcaa tagtatattt
     6421 gatcttattt ttgataaaag ctagtaatat gtatgtattc ttaattctta gttcggttgg
     6481 ctatatgggt ttgggtgtgt ttaatactgt tatttgggca aatattactg atgttattga
     6541 tgatcaagaa gtaaagtctg atcaaagaga agatgggacc gtatatgcgg tatattcttt
     6601 tgctagaaaa ataggtcaag ctctagctgg aggcgtcgga ggttggactc tttctattat
     6661 aggctatgac tctttagcaa atgtacaaac gggagatgtt ttagaaaagt tatataacgc
     6721 ttcaacatta attccatcaa tttgttttct gattgtagga ttaattttat ttttccttta
     6781 tccattatct aaagataaag tcttagaaaa ttgcgagctt ttaaaacaaa aacataaata
     6841 ggagagaaat atgaataatt ggatattttg tattgattca gatggctgtg ttatggatac
     6901 gatgacttat aaacacaatc tctttttcgg accaattgct gcagagattt ttgatgttca
     6961 tcatagagaa aaattcctac aggattggaa taatattaat ttatattcag agactcgtgg
     7021 aataaacaga tttttaggtt tagttaaaac attagaaaaa aatgatgtaa catcgattaa
     7081 tcgattaaag cactgggtat ctctaacaaa agaattgtct aatccatctt tgaagaggga
     7141 aatagaatat tctcagtcag aggatttatt aaaagcttta aaatggtcgg aagaagtcaa
     7201 tcaacagatt agtcaagcaa aaggattaga taaacccttt ttaagtgcta gagatactat
     7261 aaaagctctg aaaaaatatg ggaagataat tattgtaagc tcagctaata aggaagctgt
     7321 tcaagaagaa tgggaaagac atgaattatt atcattagtt gatgaactgt gttgtcaaga
     7381 taaaggaaaa aaagaagata ttattcgaag tgtaattgaa aatggttgtg atttggataa
     7441 gatattaatg attggtgatt ctcctggtga tctagaagct gcaaataaaa ataaagtttt
     7501 cttttatcct attttggtca ataaagaaaa agaatcatgg gaaaacttaa gaactgatgt
     7561 tttatttaaa tgtttatcgg gagattatgt agatattgaa caaaaatata tagatacttt
     7621 ttggaaaaat cttactaata agtagaggag aaagaaaatg actataaaag tagatttacg
     7681 aaaaaaacca tataatttag atgaagatgc tatttcttgg attgaagaga cgattgattc
     7741 tatgactttg gaagaaaaaa taggtcaatt gtttgttaat atgggatcta gtcgtgataa
     7801 aaattacttg agtaatatgg tcaatcagta ccatattgga gctgtacgat ataatccagg
     7861 aactgctgat gaggtatatg atcaaaacta tatcttacaa acagaaagta aaattcctct
     7921 attaattgct gctaatacgg aagcaggtgg taacggagct tgtacagatg gtactgaagt
     7981 tggtgtagaa gtaaaaattg gtgcaacaaa tgatgcgaaa tatgcttatg aattagggag
     8041 agtttctggg attgaagcat cggctattgg atgtaactgg agttttgctc ctattgtaga
     8101 tattaataga aattggagaa atcctatcat ttctagtcga tcttttggac aaaaccctga
     8161 taaagtttta gaacttagtt tagcttatat gaaaggtatt caggagagtg gaattttgcc
     8221 tgcagctaaa catttccctg gtgacggtat tgacgaacga gatcagcatc tatcttttag
     8281 tattaatagt ttaggtacgg aagaatggga taattctttt ggaaaagtat actcaggctt
     8341 aatagaagct ggattacctt caattatggc aggtcatatc catatgccag cttattcaaa
     8401 acattttaat cctgatctga aagatgaaga tttacttcct gcaactttat caaaagaatt
     8461 aatcactgac ttacttagag gaaaactcgg atttaatgga ttagtagtta ctgatgctag
     8521 tcatatgtta ggaatgactg gggcaatgaa acgatcagaa ttattgccca cttctattgc
     8581 agctggatgt gatctatttc tattttttaa tgatccagat gaagacttta attatatgat
     8641 ggaaggctat cgtaaaggaa ttatcactga tgatcggtta ttagatgcct taaaaagaat
     8701 tttagggtta aaagctaaat tgggattaca caaaaaagca aaagaacaaa ttttattacc
     8761 taagcaagaa gctctggaaa aaattggttt aattgaaaat aaatcaattt ttaaagaggt
     8821 ggctgaccaa gcaattacat tagttaaaga taaacaagat atttggccta ttgtaccaga
     8881 aaaatataaa aggattttac tagtagatgt acatggggta aaaggtgggt ttggtgaagt
     8941 agttgctgga aatagacctc aagccattga tttattgaaa cagattttgt cagcaaaagg
     9001 atttgaagtt gaaaaataca tttcacctga agaagaattg aaaggattat cgcaagaaga
     9061 aaaaattaaa aatatagctg gaatatatgc tgctaagaga ccaattaaaa atttaactaa
     9121 aaaatatgac ttgatcatta atatagctaa tgttaatcca ggcacagttc aacgaattgt
     9181 ctggccagca agtaaaggaa ctccggatat accgttttac attcatgaaa ttccaacaat
     9241 ttttgtatct gtacagtgtc cgtttcattt ggctgatgta cctcaagtaa agacttatat
     9301 taatacttat gatggaaaac aagaaacttt agaaatattg gtggacaaac taatgggtct
     9361 ttctgaattt aagggagtaa gtccaataga tgcttattgt ggtttaaagg atacatttat
     9421 ttaatattta gataggagtg gtttatatgt tgtatcccat tatgacagag acgaggcaag
     9481 taattgactt aagcggtctc tggagattta aattagatac aaatgatgac ttacaggaag
     9541 aacttgcttt agaaccaata aatgaaaaag agagttatcc gatttcagtt ccctcttctt
     9601 ataatgattt atttgaaaca gagaacatta gaaatcatgt tggatgggtg tggtatgaaa
     9661 aagaagtgac tcttcctaaa agattattag aagaaagatt agttcttcgt tttggatctg
     9721 caacacatga agcaaaagtt tttttgaatg gaaagtttat tactcaacac aaaggtgggt
     9781 ttacaccatt tgagtctgaa attaatcggt ttgtaaaaga cgggaataat cgaattacag
     9841 ttgctgtaaa caatattatt gacgatagta ctttacctgt ggggattatg aaggaaacag
     9901 tacaggaaaa tggggatata aaaaaggaaa acttagttaa ttttgatttc ttcaattatg
     9961 caggtattca tagacctgtt aagttatata caactccgaa agactatata agtgatatta
    10021 ctattaatac aagaataact gatttggatg caagtgtaga attcaatatc gatacagaat
    10081 ggaccggaaa aatcttaata gcgatatatg acgaagaggc tcaacttgta gctgaagctg
    10141 aaggaaagaa aggaatgatt cagcttaaga atccaatttt ttgggaacct ctagacgctt
    10201 atttatatag tatgaaaatt agtttaattg atgataataa agtgatcgat acctatacag
    10261 aagaatttgg tattcgcagt gttcaggtta ccaatggcaa atttttgatt aataataaac
    10321 ctttttattt taaaggattt ggtaagcatg aggattcgta tgttaatgga cgtggtttga
    10381 atgaggctgt aaatattaaa gattttaatt taatgaaatg gataggtgct aattctttta
    10441 gaacatcaca ttatccttac tcagaagaaa ttatgcgatt agcagatcga gaaggtattg
    10501 tagttattga tgaagtacct gcagtagggt tacatttaaa tttcatggcc actttattag
    10561 atgataatgt ggaaaaacat aatacgtgga aagagatacg aacagaaaat tctcataaaa
    10621 cggtgatcaa ggaattgata gaacgcgaca agaaccaccc aagtgttgta atgtggtcta
    10681 ttgcaaatga accagattcg gattccgaag gtgctaaaga atattttgaa cctttagttg
    10741 aattaactaa agaacttgat ccgcagaaaa gacctgtaac aatcgttact tatctaaaat
    10801 caacgccaga tgtttgtaag gtaggagata ttgtcgatgt tttatgttta aatcgttatt
    10861 atggttggta tgtttcggga ggggatctgg cacatgcaaa atatctgttg aaaaaagagc
    10921 tagatggatg gatggaaaga tgtcctgata cgccaataat gtttacagaa tatggagcag
    10981 atacagtagc tggatttcat gatacagtac caacaatgtt tacagaagag tatcaagtgg
    11041 cttattacga agcaaatcac gaagtaatag atacatgtga aaattttgtt ggtgaacaag
    11101 tgtggaattt tgcagatttt gcaacaagtc aagggatttt aagagtgcaa ggaaataaga
    11161 aaggaatctt tactagagat agaaaaccta aaatgattgc tta
//
LOCUS       DASJON010000092        11193 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJON010000092 DASJON010000000
VERSION     DASJON010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.092"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..902)
                     /locus_tag="U3989_001737"
     CDS             complement(9..902)
                     /locus_tag="U3989_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1715188.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(886..1572)
                     /gene="ftsE"
                     /locus_tag="U3989_001738"
     CDS             complement(886..1572)
                     /gene="ftsE"
                     /locus_tag="U3989_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN1715189.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1677..2778)
                     /gene="prfB"
                     /locus_tag="U3989_001739"
     CDS             complement(join(1677..2705,2707..2778))
                     /gene="prfB"
                     /locus_tag="U3989_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN1715190.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2904..5438)
                     /gene="secA"
                     /locus_tag="U3989_001740"
     CDS             complement(2904..5438)
                     /gene="secA"
                     /locus_tag="U3989_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN1715191.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5660..6211)
                     /gene="raiA"
                     /locus_tag="U3989_001741"
     CDS             complement(5660..6211)
                     /gene="raiA"
                     /locus_tag="U3989_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN1715192.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6339..7058)
                     /locus_tag="U3989_001742"
     CDS             complement(6339..7058)
                     /locus_tag="U3989_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN1715193.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7055..8368)
                     /locus_tag="U3989_001743"
     CDS             complement(7055..8368)
                     /locus_tag="U3989_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1715194.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8449..8769)
                     /locus_tag="U3989_001744"
     CDS             complement(8449..8769)
                     /locus_tag="U3989_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715195.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8877..9164)
                     /locus_tag="U3989_001745"
     CDS             complement(8877..9164)
                     /locus_tag="U3989_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715196.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9291..9935
                     /locus_tag="U3989_001746"
     CDS             9291..9935
                     /locus_tag="U3989_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN1715197.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9994..10701)
                     /locus_tag="U3989_001747"
     CDS             complement(9994..10701)
                     /locus_tag="U3989_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1715198.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10921..11091)
                     /locus_tag="U3989_001748"
     CDS             complement(10921..11091)
                     /locus_tag="U3989_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715199.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3051 a         2430 c         1956 g         3756 t
ORIGIN      
        1 ttgtggtatt agaatttcaa aaaacgtctc atagaaatga tcgagccaag tgaaccaatg
       61 atcacaccaa tgccgaccat taaaagattg attttccaaa cgatattttg tggacgaatc
      121 aatgaataat gggaacgtaa taatgatgga ttgatcaatt ggaaaaacca tgcataacca
      181 aatgtgatga tcaaaatcgg aagaatcgca ccgatcagac cgatccagcc accttcaagg
      241 aaaaacggcc agcgaatata tccatttttt gcaccgacta aacgcatgat ttgaatttca
      301 cgctgtctag acaaaatcgt gatccgaatc gtgttcgaaa tgagaaacat cgctacgaac
      361 agcagcaatg ccgctgcaac taatccccat gtccgaactg caccagcgat ttggaaaatc
      421 cgatctgagg aagctccacc gtaatcagaa cgagaaacat tcttcaaatc tgctgcttct
      481 tctgctactt gttttgtata agaaggatca gtagcactta ctacatacac atcatataac
      541 gggttattgt caccttcaaa caatttccag gaatcaccca tttcgttttg gatttttttc
      601 aattcctgtt ctttgcttga aaattcaact tttttaacat gatctaagtc attcaattct
      661 ttttctagct ttttcatctc ttcgtctttt gtaccgatat ctacgaatac agatacatca
      721 acgtttcctt ctatatcctg agctaattta gttgcattca tgataactgc catgaataca
      781 ccgactaaag tcaacgtgat cgttaccgca ctgactgaag caactgtcat ccagccgtta
      841 cgtttcaaac tcttgatact ttctaataaa tggcggaaaa aggttctaat catcgtatcc
      901 atattcccct tccgcttgat ctcgaataat gcggccgttt tcgatagcaa ttactcggtg
      961 ccgaatcgta tttacgatcg tactgttatg tgtagccatc acgacagtag ttccttgggc
     1021 attgatccga tctaataatt tcatgatttc ccatgagttt tctggatcca gatttcctgt
     1081 cggttcatcc gcgataagta ctttaggcgt attcacgatt gcccgagcaa tagagacacg
     1141 ttgctgttcc ccaccggata attcgctagg aaagactcga actttgtgtt tcaaaccaac
     1201 aaggtccagc acttccatta ctcgtttttt gatatctcga ggttttctgc cgataacttg
     1261 catagcgtac gcaacatttt catatactgt ttttcttggt aataatttat agtcttggaa
     1321 caccacgccg atttctctgc gtagataagg aacttcgcgg tttttgattg ctattagatc
     1381 atgacctgcg acgttcaaag ttccttttga agctttttct tctcgataca tcaatttgat
     1441 gaatgtagat ttaccggctc cagacggacc aaccacatag acaaactctc cttgttcgat
     1501 gacaatcgag agattgcgga tagcggtcgt accatttgag tacttcttca ttacatcctt
     1561 catttcaatc atggcgttct ctccatatct ttttcagtct ttagctatta tagcatggca
     1621 atgtttcaac atgcttgcaa aatcttacaa ttttatttca atttaacaat gagttcttat
     1681 tgtagatgca tttttaaata gccgtcaata aataaatcaa ggtcaccgtc cataactgct
     1741 tggacatttc ctgtctctac gtttgtccga tggtctttta ccatagaata gggatggaaa
     1801 acataagaac ggatctgtga accccagccg atttcttttt gttctcctcg aagtgcagct
     1861 gcttcttgtt ctttcttttc cacttctaat tgataaagct tagcttttaa catccccatc
     1921 gcttgctcac ggtttttcaa ttgtgagcgt tgtgcctgac tagctactac tgttcctgta
     1981 ggcaagtgag tgatccgtac agcagattct gttttattga tgtgctgacc acctgcacca
     2041 cttgctcggt acgtatcgat tttcaaatca tctggattga tttctatatc gatcgtttca
     2101 tcaagctctg gcatcacatc taccgaacaa aaagaagtat gacgtcgttt cgctgagtca
     2161 aaaggcgaaa tccgtacaag gcgatgaaca cctttttctg attttagata accaaaagcg
     2221 ttatgtcctt taatcagcag ggtcacactc ttgatccctg cttcatcacc tgcttgataa
     2281 tctagtgttt ctacttgata gccatgctgc tctgcccaac gtgtatacat acgtaaaagc
     2341 atgctgcccc agtcttggga ctccgttccg ccagcccctg gatgcaattc gacgatcgca
     2401 ttgtttcgat cataaggtcc gtctaacagc atagataatt catacgtctt cattttttca
     2461 tctaatgcat tgatacgtgt ttctgcttct tcttgcattg ctgcatcagg ttcttcttgc
     2521 agcatctcca ccatcaattc gatttcttct agttcttcgg caagagaatg gaattggtca
     2581 taggtttctt tattagcgtt attttcgctg atcacttttt gtgcggcttc gctgtcatcc
     2641 caaaaaccag gttcagccat ttttgcttct gcttccgcga tgtcttcttc cagctgatct
     2701 aagtcaaaga gacctcctga aacttgtgac cttttcctgc atttctgtca ggagatttcg
     2761 aatttcggat atttccatgt tttttccttc tttcactata cttttttcat tatatctagt
     2821 ctaacagaat cagcaaataa cagaaagaag gtgagacgaa ttcttaaaaa aagaatttgt
     2881 ctcaccaatc ctgctgtcag aaattagttt gcattttttc catgacagtt cttatatttt
     2941 ttcccgcttc cacaaggaca aggatcattt cttccgacct tgttcacatg gattggctgt
     3001 tttttcgcgc tagtattact attttctttg gcatctccgt cttctgagtg agaagctgtt
     3061 ccttgagaaa cctgttcacg ctgtacgttt tgacggatct cagctttcat aaagattcga
     3121 gtgacttcat actcgatggc gcctaccata tcttcaaaca tcttatagcc ttctgtttga
     3181 tattcgacta atggattgtt ctgtccataa gcacgtaagc cgattgactg acgtaattga
     3241 tccattgcat caatatggtc tgtccattta gagtccacaa cacgcaagat cacgactttt
     3301 tcaaattcca acagttgctc tggaccattt agttgagccg ctttggtatt aaatacttct
     3361 tgtgctcgtt cgtttagata ctctttgatc tcctgagatg tttttccttc gatatctgca
     3421 agagtaatac tatcttcatg gacaagagta ctgccggcaa aatcgatgat actttccaaa
     3481 ttccaatttt ctttctctaa ttgtgtatgc gcatctacta cacgactgat tgtccgtttg
     3541 accatgttca ttagtggctt ggataagctt ttttcttcca tgattacttc ttgtcgctgt
     3601 ttatagatta cttcacgttg ttcacgcatc acatcatcgt attgtaagac gtttttacgt
     3661 gtgtcatagt tatttccttc tacacgtttt tgtgcagatt ctacttgttt cgtcagcatc
     3721 ttgctttgga taacagcatc ttcctcaccg atcttcatcc gatctaagaa agctttgatt
     3781 cgttctgaac cgaatcgttt catcaaatca tcttccaatg acaagtagaa ttgtgacata
     3841 ccagggtctc cttgacgtcc tgcacgtcct cgaagctggt tatcgatacg acgagattca
     3901 tgtcgttctg tcccgataac tgctagacca ccaagttctc ttaccccaag tcctaacttg
     3961 atatctgttc cccgaccagc catgttggta gcaatcgtca cagcaccttt ttgaccagca
     4021 ttcatgatga tctctgcttc tttaaagtga tttttcgcat tcagcacttc atgaggaata
     4081 cgttcttgat tcagcatatc tgataataac tcagatgttt caaccgcaac agttccgact
     4141 aacacaggtt gacctttgcg gtagcgatct ttgatatctt gcacgacagc gtggaacttg
     4201 ctttgtaatg ttgggtaaag taaatctgga cgatcatcac ggataaccgg acgatttgtc
     4261 ggaatctggt atacttggat gttataaatt tctctgaatt cttcttcttc tgttttagct
     4321 gtaccagtca ttcccgcaag tttcttgtac atacggaaat agttctggaa agtgatcgtc
     4381 gccatggttt tcgtttcgtc ttcgatctct acgccttctt tagcttcgat tgcttggtga
     4441 agtccatctg aataacggcg tccatccatg atccgaccag tgaactggtc aacaatcagt
     4501 acttttccgt cttggacaac ataatcgata tctaaaagca tgatatagtt ggctctcaat
     4561 gcttgatcca agtgatgggt caatgcagta ttttcaatgt cgtataagtt atccaaaccg
     4621 aatgtttctt ctgctttttc gatccctgct tcggttaaac tgattgtttt tgattgtaca
     4681 tcgattttat aatcttcttc ttctttcaaa cgtttcacga agttgtctgc tcttgtatat
     4741 aaagctgtgg atttttctgc ttgccctgaa atgatcagcg gcgttctcgc ttcatcaatc
     4801 aagattgagt caacttcatc gacaatggca taattcagcg gacgttgtac catttgatgg
     4861 cggtaaacga ccatattgtc tctcaagtag tcaaatccta attcattatt cgtactataa
     4921 gtgatatcac aattataagc ctcgcgtttt tcatccgaag attttgagtt gatattcaac
     4981 cccactgtta atcctaagaa attataaagt tcgcccattt ccgtcgagtc acgggttgct
     5041 aaatattcat tgacggtaac tacgtgtacc ccttcgccag tcagagcatt tagatatact
     5101 ggcatcgttg cggtcaaggt tttaccttca ccagtacgca tttcagggat atttccatcg
     5161 tgaagcacga ttccacccat caattgtacg tggtatggat ataaccccaa tacacgtttt
     5221 gctgcttctc ggacgactgc aaaagcttcc ggaagtaatt gatctaatgt ttctcctttt
     5281 tggtatctgc ccttgaattc agcagttttt gcctgtaatt gttcatcagt taatgctgcc
     5341 atttggtcag cgtatgcttc aacttttttt gcaatgctat ctaaacgttt tagttctttc
     5401 ttatcgtttt cgataagtga acgaatcaaa ttggccatgc gttgtgtaat ctccttttcc
     5461 ttctgtgtat tatccagact atgtagactt tacttttcat tttatcatta gttatagata
     5521 attgaaatat cttaagcgta ttaaagtaag aacttcataa tttattcata tttcatctaa
     5581 aagaggagga aactcaaaaa aacttgcggc agctgtcagt agctgctgca agttttcgtt
     5641 gttatttaag taaggctgat cagtctgttt caatcagtcc gtattttcca tctttacggc
     5701 ggtaaacgat acttgtacca tttgtttctg catcttcaaa aataaagaag ttatgtccca
     5761 gcatgttcat ttgtaagact gcttcttcac tatccattgg tttcaatgaa aggcgtttcg
     5821 tacgtacgat atctaattca gaatcatttt cgttttcgtc ctcttcattg aataaagcag
     5881 cagcatctgc agtattcatg cccgtttcac gggatttacg attaattttt gttttgaatt
     5941 tgcggatttg gcgttccaat ttatcaacaa ccaaatcgat acttgcgtat aaatctggag
     6001 aagtttcttc ggcacgtaat acaagataag gtaatgggat cgttacttcc acttttgctg
     6061 ttttttctgt ataaactttt aagttcacat gcgccgttgc atcgggtgcg tcactgaaat
     6121 agcgttctag tttgcctact tttttctcaa cgtagtcgcg aatcgcttca gttacttcga
     6181 tattttcgcc gcgtacatta tatctaaaca taaaaattac ccctttcgtc taccacaatc
     6241 aagaaggaga aggaccactc tttccctctt cttaattcta ttatatcgaa ctctaggtta
     6301 tttgcaaatt aaatcgctat caaatttcca catctaattc atctcgccaa agaaatggtt
     6361 cggatgattt tcggctgtgc gacctgcaaa caatctgctg cgtggaaaag agtctgtcct
     6421 gttgtataga catcatcaat aatcaatatt tttttatttt gtatcttaga aaaggtgact
     6481 gcaagttcaa aaggttgagg catctccaat ctttcctttc gcgtgttttt tgcttgagcc
     6541 agcagatgct tctctctttt gagtagcatc tgaaaaggaa ttcttgctgc tttcaacatc
     6601 tcgcttactt gattgaatcc tctttctgtt cgcctttttt cagataaagg tattggacaa
     6661 tagatgtatg tcgggtattc tttttttatc tgcgtccatt tatatgcaaa ggtgcctgct
     6721 agtcggatat ctcccataaa cttgtattga ttgagccagg ccttcatatt ttcatcgtac
     6781 acaaaaaagg cacggtgctg aaaatcatag gtcggatatt tcttcttcca ttcttgacag
     6841 tcactgcagt aaacgggaat agactttact ttctttttcg tactctctag attgaaatgg
     6901 attggttttt gacagccaga acagcctctt gtttccaata aatgaaattg tgcgcgacaa
     6961 actgaacaag tctgaggtaa tctgtatttc gaaacaccaa taatctcgct gaatgttaaa
     7021 ttttggaaga tatctttact gcagcaactg cacctcacag catcttcttc ccttcttcat
     7081 tcatttcttt gatttcctga caagcttttc gaatagctgc agttttttct tcatagaaaa
     7141 agtatacttg actgttcgta taaggccctt tacgatcggc tcttcctgcg atttgtacca
     7201 aagaagattt agtaaaaacc ggatgatccg ctcctaaaac gataacggat attctttcaa
     7261 atgtgactcc acgttcaagg atcatcgtgg ttaacagtat atcatatttt gcttctcgca
     7321 ttttctggac tttttccgcc cgctgattgt ctcttgaaga aacatctgtg atctcatatc
     7381 tcggaagagc ctcttgaagt gtggctttca aacgatgcat catcgtgata cttggacaaa
     7441 ataaaaggat atggttttct ttaattaatt gatgaatgat ccgcaccaaa gattttatct
     7501 tcttcttgtt cttgcatttt tgtcgccaat tattcatgaa aaccaagacg ggttcaggaa
     7561 gcaaacgccg atgaaagcgt aaagcaagtt tgtggatgtc aaacgtctgc ttgatttcct
     7621 ttagtaactt tttatcaggt gtggcactta agtagatgaa tcgtccgtct ggtttaatcg
     7681 ctgtttgcac agcatgagct aatcctgcat ccccgttata aggaaacgca tcgatctcat
     7741 cgatcactat taaatcaaag gaccgataaa aacggtatag ctgatgaatc gtacagataa
     7801 caaaagaagt aaaggtataa gccgttgctg tattcccatg gagcaacgtg attttttctt
     7861 ctggaaaaac ataacgatag cgtaaaaaca attcattaca aacatcgact cgcggtgtac
     7921 aaatcgctac tcttcctcca gcttttaaag tttccactaa aataggataa agcatctctg
     7981 ttttccctgc acctgttaca gcccaaataa gatgatgtct tttttctgaa tggcaaagta
     8041 ttctctttga gatttccgtt tgtttttcag taagctgtcc tgtccaaaaa aagacgactt
     8101 ctcgaggttt tgcttcaggc tgatcaaaca aatataaatc ttgttgagtg tcgcatcgcc
     8161 ctaaatatag acaatgtgta caaaaataac cgtaatctgc aagctgtcca tcaatgacgg
     8221 acgtcctcat tccacagcga tgacaaagaa tccagttgcc caaacgttcc actgctgcga
     8281 tcttctcata atttggtaaa tttacctcct cacagtctat tgcttcaata tctctcgtca
     8341 gaagccttct accccaaaaa tgattcatat tcttcgccct aaaaaaatat acgaaaaaac
     8401 aagcgatcta ttttttggat cgcttgttcg atagttatac tgttctattt attcacctat
     8461 gagcgtgatt gcttgatcta acactttttc gccattcatc ataccataat cagccatatt
     8521 gatgacatct aaaggaatgc cttttggttc cagacgtttc tcaaaatcag ctttcataaa
     8581 gcgaacttgt gggcctaata acaacacatc cacgtcttta tttgcaaggt tgttatctgc
     8641 gtcagaagcg gacacagcga aaatatccga gtcgattcct tttgcttcag cagctttttg
     8701 catttttgtt actagtaaac ttgtgctcat accagccgaa catacaagca tgattgtttt
     8761 tttagccata cttttctctc ctttttaaaa caaactatgc aaacgttttc ttcgcgtctg
     8821 ttactctatt atataaaaag aaagaaattt gtcaacgttt tctaaacgcg gtttatttaa
     8881 tcaaatagcc aactagattt cactaattta ttgaacacga catattcttt gtccaaattg
     8941 atgacaacta cttcatagcc tgcatttatc cagccatagg cacctttttc agggtgttta
     9001 taatcatttg cccaccaatc tgtactttcc cttgctgttt tgggaagtcc gttactggga
     9061 aacagcagtt cgtcaaattg ggtaaaggtc aacgttactt gagagccgcc attgttcttc
     9121 aaataatgcg taattgcatc gtactttttc attcgttcct gcatccccag atccacctgc
     9181 ctttgtcttt attatcgcat actacagcaa ttgcgcaacc gttacagtgt tgaaacaacc
     9241 tagaacttta tttatactga ataaagaaaa tgaaaaaaag aggtgacaag atgatcgagc
     9301 gctatcgtac aataaaagaa aatggacaaa gtgagattga gatcaaaaaa tcacgcttta
     9361 tctgttctct tagacgaata gaatcagagg accaagcaaa aagtttcatt caagaaataa
     9421 aaaaagaaca ttggaaagcg aaccataact gcagcgcctt catcattgga gaaaaaaatg
     9481 aaatccagcg aagcagcgat gatggagaac caagtggaac ggcgggcgta ccgatgttgg
     9541 aagtcctcaa aaaaaatcaa ctgatcaatg tagctgcagt agtcactcga tattttggag
     9601 gaacgaaact cggcacaggc ggattaatac gagcatacac tcatgcggtc tctcaagcat
     9661 tgtctgacat tggaatcgta gaaggtacct tgcaacagga gatctctgtg atgatcacgt
     9721 atccgcaact aggaaaatta cagaattatc ttgaactcca acattttttt ataaaagaaa
     9781 tccaatatac tgatcaagtt agcgtcatct gtgctgtaga tgaaaaaagt gctgaccact
     9841 ttaaagaatc catcattgag ctgctgaacg gacaagtcca atttgaagag ggttctgttt
     9901 cttatcacga gcaactgata actgatcata tataaaaaca ggtgattctg attctctttt
     9961 atttctaata aggaatcagg gatcacctgt tttttatttt tccaaataaa actcaaaacg
    10021 atttcctacg tattgcgtac gcacatactc aaacggcgta ccgtcttcaa aataagaaat
    10081 ctggcgcaaa cgaagaactg catctcctcg ttttacctct aaatagtcag caattttctc
    10141 agaagccaac acagcagtaa tcgtttgatt cgctggaccg atctttttcc cgcctttttc
    10201 ttctagcgtg cgatacaacg attcagtaat ctctgctttg ctgtagccat cgatcagctt
    10261 ttgtggaaca ctggcaactt caaaacaaat agggacatca tctgcgaaac gaatacgttc
    10321 cattcgcaag atcgtgtcat ctttactcaa gtttagtttt tccatttcac ttgagcttgg
    10381 cgaagtcaag aaataagaga ttgtccgact agacggtact cgattttgtg attccataat
    10441 ttctgtaaaa cttgttgcac cagacatttt ttcttgtact ttttctcgcg caacatacgt
    10501 acccgaaccg atctttcttt ctaagattcc ttcatctgcc aagttttgaa ttgcttgtct
    10561 caacgtcata cggctgacat tgaatttcac tgctaattct ctttctgaag gaagacggtc
    10621 tccgactttc catataccct gttcgatttc attttttaat tggtcatgaa tctgtatata
    10681 tacaggtaat tgattcgaca taacgtacac ccgtcctttc gtttcttcta ttataactgg
    10741 tatatacctg attcacaact gaaaaagata aaggtttcta tccatcaaca gaggatttag
    10801 acacacatcg ttctccctac tcctattgtc agaaacaatt ttttagatat gttcaaccta
    10861 gaaaataaca aaagacccta aaatcgatga aaatgccgac ttatggatct tttgttatta
    10921 ttagcggttt ttagccatcc atttttttgt atgattcgtt gggcgttttg attttacctg
    10981 gaccgctttt ccgccttttt tcggaatatt cacttcaata ttgtattttt ctgatttgac
    11041 caaatcatat aacttttcag ctgcttcgtc tactttttct ttccatttca tgtggatccc
    11101 tccaattctt gtagttaact taactctatc ttaattcagc agaatcttca aggaacaagg
    11161 tagcaaagca aaaaaaagag ctctcgaact aac
//
LOCUS       DASJON010000093        11172 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJON010000093 DASJON010000000
VERSION     DASJON010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.093"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..752)
                     /locus_tag="U3989_001749"
     CDS             complement(57..752)
                     /locus_tag="U3989_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1715200.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            906..1184
                     /locus_tag="U3989_001750"
     CDS             906..1184
                     /locus_tag="U3989_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715201.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1203..2411
                     /locus_tag="U3989_001751"
     CDS             1203..2411
                     /locus_tag="U3989_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1715202.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2559..2924)
                     /locus_tag="U3989_001752"
     CDS             complement(2559..2924)
                     /locus_tag="U3989_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN1715203.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3071..3844)
                     /locus_tag="U3989_001753"
     CDS             complement(3071..3844)
                     /locus_tag="U3989_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1715204.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4018..4878)
                     /locus_tag="U3989_001754"
     CDS             complement(4018..4878)
                     /locus_tag="U3989_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN1715205.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4878..6296)
                     /locus_tag="U3989_001755"
     CDS             complement(4878..6296)
                     /locus_tag="U3989_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1715206.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6353..6802)
                     /locus_tag="U3989_001756"
     CDS             complement(6353..6802)
                     /locus_tag="U3989_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1715207.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7247..7510)
                     /locus_tag="U3989_001757"
     CDS             complement(7247..7510)
                     /locus_tag="U3989_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN1715208.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7609..8904)
                     /gene="rho"
                     /locus_tag="U3989_001758"
     CDS             complement(7609..8904)
                     /gene="rho"
                     /locus_tag="U3989_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN1715209.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8901..10172)
                     /locus_tag="U3989_001759"
     CDS             complement(8901..10172)
                     /locus_tag="U3989_001759"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1715210.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10337..>11172)
                     /locus_tag="U3989_001760"
     CDS             complement(10337..>11172)
                     /locus_tag="U3989_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN1715211.1"
                     /translation="ARKGGYAVGGFNTNNLEWTQAILEAAEAKQAPVLIQTSMGAAKY
                     MGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTSVMFDGSHLPFEE
                     NLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECKQMVATGIDYLAC
                     GIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQIEKAISMGISKV
                     NVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDAKSHIDWFGSANK
                     A"
BASE COUNT         3117 a         2362 c         1947 g         3746 t
ORIGIN      
        1 ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tgttcatcaa
       61 aagataatat tgtcttctaa caagtccata ttcaaaataa cgatatgatg gtctttaacg
      121 gtgataatac catctttttt cagttgtttt aaaattctgt ttacactgct agcagttgag
      181 atgccacaaa atttggcgat ttcttcattc gtgatcgaaa aatcgatcag catcccttct
      241 tctgtccgca caccgaataa atcatataat tcgtaaagtt gcgtacaaac agctcccagc
      301 tttccgttta atagcatttg ctgcattttt ttcatcgaat aaagcagacg atagcggtaa
      361 tactctttta catagtgttg tagctttggg tcttggttga tattttgcca aaagcgcaca
      421 cgattgattc gataaaactc tgcatattct gattcaactc gaatattata cggcgaatcg
      481 atgaattgtg aatattcgtc ttttaaaaga gagacgattt caagatcttt gatatagcga
      541 aggttaaatt ctctcccatc atttgaaata acacttgctt ttaccgttcc ctctttcaag
      601 atatacgtat gggaatcttc catcccttcg aatgtcaaat atttatgata tcctttgcgt
      661 ataatcggaa aatcattttt atccaaatat tcctttaaga ctctactatc caatgatgct
      721 cccccatcca aactttcatc taaaaagatc atacagtact ttaaaacttt ttaaagtcca
      781 aaggtatttt actaatgata ttaacatttg ttaagtacct ttttccatca cttatgcgat
      841 ttttttcctt ttttttattc gttatactgc agaagtaaaa ttttttaaga atagagggaa
      901 aaaacatgaa ttactggatg aacacaatca ttaatcgatt agaaacagca tatcaaacaa
      961 gatttgatat gaaagcttct ttagtctttt taaatgatgc gtatcaaaac tcgatcgaat
     1021 tgatcaaagc agtggatgag aatccaacaa acgaatgcga agaattttta aatctgttta
     1081 tgtctacaag agatttattt attcgccaac ttgttgatcg ctatccatcc aattatcacg
     1141 atgtagaagt tcaaatacaa aaattaaaag catattcagc ttaaggggaa tgtataacat
     1201 acatggaaaa gaaattattt aataaatatt ttattggaat cacattgatc aattttattg
     1261 tctacctgat ttattatctg ttgatggtca tcatggcagt cattgcgcaa gacaccttac
     1321 acgccagctt gagccaagca ggttttgctt ctgggatcta tatcatcgga acattgctgg
     1381 cacgcttatt catggggaaa cagttagaac tatttggtcg gaagtttacc ttaagaggcg
     1441 gcgcgatctt ctatctcctt tcaacattag catatcttta tacgccaact atcggggcat
     1501 tgtatttagt tcgattcttg aatggattcg gctacggtac agtttctaca gctacaaatg
     1561 ctatcgttac tgcatatatc cctgcatcta aaaaagggga aggcatcaat tattatggat
     1621 taagtactag tttagctgcc gctgttgggc catttttagg aatgattttg ctgaatatga
     1681 ctgattttcg tttcattatc tggttttcaa ttgtactggt cttctttgtt acgattgcct
     1741 gccttgtctt tcctgtaaaa aatattccat tatctgaaga acaaaaaata tctcttcgct
     1801 catggaatat caatagtttt attgaaaaga aagcactttt cattacagga attgcctttt
     1861 tgatgggtct ttcttattca agtgttttat catttttatc ttcttataca aaagtaatcc
     1921 acttagttgc tgctgggtca tttttcttcg tcgtatatgc tttggtcatc acatttacac
     1981 gtccattgac tggaaggatt ttcgatgtca aaggagaaca atacgtgatg tatcctagct
     2041 atttattctt aacagcagga ttatttttac tgagtgtaac gacgaatagt gtgacattgc
     2101 ttatctctgg agcattagtc ggcctaggtt atggtacgtt tatgtcaaat ggacaggctg
     2161 tttgtttaaa aatcgtccgc gaacatcgaa tcagtattgc tttatctacc tactttattg
     2221 gtttagacct aggcttaggc gttggtcctt atttgatggg gatgctaaaa tctcttactt
     2281 catttagagg attgtatgtg attgccggag tcattccatt gatatgtaca gcactctatc
     2341 ttgttaatgg ccgaaaacat agtcatgaag cagagggcga attgattgaa tcattgaaaa
     2401 atgaaatttg atcttgatga atagataaaa aaaaccgttc agatctgaaa ataatctgag
     2461 cggttttttg agtaaaaata gtttatgatt attcttaatt aaacatctat ttatttttaa
     2521 agctttcatt tatcgataag aatagtttaa ctgttgattt attccatttt ccgttcatct
     2581 ggtacgatgc cttgacttag caaccacttg tggaagaacc attctgccac accgatcaat
     2641 agagcaggaa tcaatacttt catccaaata tcgactgtat cattcataaa atatagatag
     2701 agccacaaga taactgcagc tgaaccagca tctgcgatcg tcgctgcgat gttccctact
     2761 ttacgtaaga tcaacagatc ccctagcaca tagatcatga ctacaataat taccccaaca
     2821 atcgttgaat ccataaagct aacaccgtat aaacctgtca gcacgaccca cagaatcgcc
     2881 cagatcatga ttgccttcac taccaacgcc ttcacatgtt tcattttctg cactcctttc
     2941 tcctggataa aaaagtaaga ttatctaaca agataacctt ttcattatta taccatcggc
     3001 tgagagaagc gagcaaagat aaggtcctat ctaaaaatga ggaagccact tagaacattc
     3061 acgtaataat ttatccgtac agacattcga tatggtgttc ctttactttc gcatactgtt
     3121 cttttgtcag ttctttactt gtcaccagat aatcgatttc atgaaaagca atggcttgat
     3181 gcatgaattt atgtgcaatt ttttgctgtg taactaaaac ggcaagccgg tctgattgtt
     3241 ttctaaaagc ttttttgacc attgcctctc cttcatgagt ttctgttact ccttggctga
     3301 aatctagccc gcgacaactc attatcgcta aatccgcata atatgacaat gcatcgttat
     3361 aggcttttga accgttgatt gttgctcgtt tcggaacaat tgcccctccc aatacagata
     3421 cttttgccgt tgtatattca ctcaacatag cagctgtgac gatgccattc gtaatgatct
     3481 gcaaaccatc aaatcttgct aggtttctga ctaagtgtaa agaggttgta ctggaatcca
     3541 agaataagac catatcatct cgaatcaaag aagaagctgt atctgcaatc ttacgttttt
     3601 ctgtttcttc ttctaatttg gtgatcttca tcaggtaagg cacgtcaatt tttcgattgt
     3661 caataagcat ggcgccaccg cgaactctac ggatcaatcc acttttttcc agaaaaacaa
     3721 ggtcacgttt aatcgtggct tcgctataat ttactaactc gatcaaatcg gtgactcttg
     3781 caaattggtt gttttttaga taattcacaa tctcctgctg tctattctcc acttcattca
     3841 acataaaatc accccattta ctttatatag taacaggaaa aaagaggttg tgacagaagt
     3901 gattggcttc gagaaataag aagaaatttt tcgaaaaaac tacttctgaa catcgtgtgt
     3961 ggttaagagg gtatgacaag actattgtca taccctcttg accacacatt tttattttta
     4021 gtataaacta gccttgccta ctgtgttcaa aatttccatt ttatgtttga ccacttcttg
     4081 taccttttta ttagctgctg gataaattgc attcggctcg tacatcgatg gattttcact
     4141 caatatttct ctcaaagcat tgaaaaagac gcttttaaga tctgagctca aattcacttt
     4201 ggcaactccg tattttactg cttcgctgac ttctgcgtcc ggattacctg aaccgccatg
     4261 taaaacaaat ggaagatcga tttgttggtt caattctttc agtaagtcga tatttaattt
     4321 tggagttctg tttttcggat acaagccatg cgctgttcca attgctacag caagtgtatc
     4381 gattcctgtt tctttcacaa aaatcttcgc ttgatcagga tctgtataaa tgattgtttc
     4441 agcacctcct tcagcagaac cattattccc aatcgttcca agctcagcct ccactgagac
     4501 atttaccgca tgagctaacg caaccacttt tttcgtaatc tgtacattgt cgtcgaatga
     4561 tttcaaagac gcatcgatca tcacggaaga atagccgttt cgaatcgccc gtaagacatc
     4621 ttctatcgta ccgccatgat ccaagtggat aacgatcggc agtttacttg tataagcata
     4681 ggctcttact gccgcaacaa actcatcact taaataggcg atttcatcag gatgaatttc
     4741 taagattact ggtgcttcta atctctctgc ttcttcgatg attgctttca tcatatcata
     4801 gctgcaaata ttgaaggctg gtactgcaaa ttgattctct ttagccactt tcaataactc
     4861 tttcattgta tataacatta cgcttccacc tcatttgtta gattttcatt tttcgtccac
     4921 ttgtcttgtt ttttctcttt cactgttttt ttcaatagtc caagcataaa ggctgtagtg
     4981 actgtacctg atgcaattgc caagacatac ataggtaaat tgttcataac atttggaatt
     5041 agcatcaccc aaatccctcc atgaggtgcc attgaagtaa ctcctactgc catggaaatc
     5101 cctgcagcga ctgctgaacc tgtcatcaaa ctaggtatca cgcggatcgg gtcagatacc
     5161 gcaaacggta tagcaccttc tgtaatatac gaagcgccta aaatccagca tgattgacct
     5221 gaagtccgtt cttctttggt aaatttatgt ttgaataata aagtggctaa agcaatacct
     5281 aggggaggtg tcattcctgc agccatacaa gcagcaatcg gtgcatttac tccagctgac
     5341 atcaaaccga tcgagaaagt cgaagtcgat ttattgatcg gcccacccat atcagctgcc
     5401 atcatgatcc cgataagtaa tccgaacaat agcccggatg tatttcccaa gctattcagc
     5461 caatttgtca aagcatccaa cacccatgtg atcggttgtt caatcacaaa taccatcaaa
     5521 agcccaataa ttaagatcga taaaaagggg acgacaatca gctgataaat agcagaaaat
     5581 tcttttttta cgctaatttt atttgccaga aaatcacaga tataccctgc tagcacgcca
     5641 ccaatcattc cgccaagaaa acctgaacca cctgtagagg ccagtaatcc ggcaacaatt
     5701 cctggggcaa aacctgcttt tccagcaatc gatgtagcaa tccccgcgcc taacgcagga
     5761 accatcatgg caaacgctgt atctcctccg atccttgaaa atgcagctgc taacacatta
     5821 tagtctgctg agttgggatc agccgctgta atcccaaagg caaaactcag tgcgatgatg
     5881 attccgcctg cgatcacaaa tggaagcatg taattcacac cattcatgaa atgattatag
     5941 attgtcggct ttttctcatt tgcagctggt tctttttcat tagacacaac gaatacgcca
     6001 atatctctca atgcttcgtc tattactact tgcggttctt tgattgctcg gcttgtagag
     6061 acacgcttta cttttttccc ggcaaatcgg tccaattcga tttctttatc tactgcaaga
     6121 atgattgcat ctgcctcttt gatttcttgt tcagataact gattttctgt ttttacacct
     6181 tgtgtttcga attttgcttg atggtttaga tttcttgctg ttacttctaa tttttctgcc
     6241 gacatataag tatgagcaat tccagtcggg caagcagtta caccaacgat tttcatgatt
     6301 attttcctcc tttttcttca ctatcgatac tcggacaaag actctactat tttcattctt
     6361 gactaatctg tctgatgata tctgctggcg tagtggcttc ttttattccc tttctaaaat
     6421 ctgcattcat caatcgtctg gagagtgtgc tgagaatctc tagatgcata tctgctgatt
     6481 tttcaggaac tacgatggca aaaatatatt caattggcat ttcatctatc ccgctatagt
     6541 ccgttaattt ggctgtatga aaaaaacaca gtgctggttt tgtcacatat ttgctttttg
     6601 catggggaat cccaaatcca tcttctattc ctgtggagat ctcctcctct cgtttgaata
     6661 gtgccttaac gactttttga acatttctta ccttatttgt ttttttcaac acatccccta
     6721 ttcgctcaaa taatgatcct tttgaatcta tcgatggatc gaaaacaatc agttcttctg
     6781 cgatcaattg atcaaacctc ataaaaaacc tctcccccac aaaaaatgtt aacgttttct
     6841 tggaactgag gtcagtatag catcgctagc tgtaccgttc aatattatga atgaacagag
     6901 gaaaaagacc catcatccgc gtgtataagt cattaaaatg aacgatctag ctcacaaaca
     6961 atcgcaagtc tataacaaaa caaaaagcct tccttgaaag gaaagcttag cttctaattt
     7021 taggtaaatg gttttttact tcgaaataac ccctcttcat ttcgttaata taaggtgttt
     7081 ttatcaagtg gataattaaa actatatact agtttttcat tgttttgtgc attatgatcc
     7141 tacgactttt tcagcatcaa acgaagtttt aaattggcaa aaaacataaa aaaacagact
     7201 atccctatca gatagtctgc taaacgttga gttatcagcg tttttattat tccgcagcgt
     7261 ttgcgtcttt gagaccgtat tttttgttga aacggtccac acgtccgtct gcttgtgtga
     7321 atttttgacg tccagtgtag aatggatgtg agtcagaagt tacttcgaca cggatgactg
     7381 ggtatgtatt accgtcttcc cattcaactg tttcttgtga agtttttgtt gaacctgaca
     7441 agaatttgaa accagttgtt gaatccatga atactactgg gtggtaatct ggatggatgt
     7501 tttctttcat gattttttcg ctcctttgcc ctgctccatt tgctgaacca gagttgattt
     7561 gtcgatttac aacattaaca tcttaacatg tttcagtcaa ggttgcaatt atcttttcag
     7621 atttctggtc ggattttttt taccaaagga gacatcttga aattctttga aaaattgctg
     7681 attgtttttt gttttacgca aaaagcggat cagttgttcc gtatattcta atgaatctcc
     7741 agtcatattt tttcgaagtt tccaaatttc ttctaattct tcacttggca tcaataattc
     7801 ttcttttcgg gtacctgatt ttttgatatc gatggctggg aagatccgac gttcagataa
     7861 ttcacgtgaa agttgtaatt ctagatttcc agttcctttg aattcttcat agatcacgtc
     7921 atccatacgg cttcctgtat cgactaaagc ggtcgctaaa atcgtcaaac tgccaccttc
     7981 ttcgatgttt cttgctgccc cgaaaaattt cttaggttta tacaatgcgg ctgggtcaat
     8041 cccgccactc aatgtacgtc cgcttggtgg aacgactaag ttataggccc gagcaagtct
     8101 agtaatacta tccatcaaga taacgacatc tcgtttatct tcgactaaac gcattgcgcg
     8161 ttccaatact aattcagaaa ctcgagtatg gttttgcggc tgcagatcaa atgtagaaga
     8221 taccacgtcg cctttcacgc ttcgttctag atccgttact tcttccggtc gttcatcaat
     8281 cagtagaacg attaattcta cttccggata gttttcggtg ataccatttg cgatttcttt
     8341 taatacagaa gttttccctg cttttggcgg tgcgacgatc aatccacgct gaccaaaacc
     8401 gattggagaa aagatatcga tcattcgggt agatagtctt tgcggtgttg tttccagttg
     8461 aagctgtctt tgcggataaa gaggtgttaa ggctggaaaa tgcggacgtt cttttgcttc
     8521 ttccggatct tttccattaa cgctttccac gtgcatcaaa ccataataac gttccgattc
     8581 tttaggtgga cgggcttttc ctgccacttt gtccccattt ctcaaactga atcgtctgat
     8641 ttgtgaggca gaaatataga tatcttctgc acttggtcca taattgattg gccgtaaaaa
     8701 accgtaacca tcttgaccaa cgatatccaa tattccttcc atataataaa atccttgttt
     8761 ctctgcttgt gcacgaatca cagctagaga taattccttt ttattcatct tactgtaata
     8821 aggaatttta aattctttgg cataactata aacttctttt aacgttttgt tctctagttc
     8881 tgccattgtc aaataatcgc tcattcagct cccacttcct ctatcatttc aattgatgcc
     8941 cctaaggctg ttagtttctc gataatatga tcataaccgc gtaagatata atctacattg
     9001 taaatcgtcg ttgtcccttc tgccaatagt ccagcaatca ctaaacaagc acctgcccga
     9061 agatcagaag ccacgacttc tgtgccgtga agtttatttg gaccattcac gatgatcata
     9121 tttccttcca cggtaatatc agcgcccatt cgtgctagtt ccggcacatg gttcacacgt
     9181 ttcgcataga tcgtatcgat gatttctgaa gtgcctgtag tcatcaatag taacgcagta
     9241 agtggctgct gcagatctgt ggcaaatcct ggataaggat aggtcatgat gtttgcgggt
     9301 tttaattctt tcgaggggta aacttcgatt tcatcttcgc tgattttcat atggacacca
     9361 atttcctgca attttgctat aaagctttcc aaatgttcaa agatcacatt tttcactttg
     9421 ataccatttc ctactgcagc tgctaatgct agataggttc ctgcttcgat gcggtcagga
     9481 atcatgaaat gccgacagcc atgtaatgtt tcgactcctt cgatacggat cacatctgta
     9541 cccgctcctc gaacttttgc ccccatatta ttcagtaaag ttgccacatc tatgatttcc
     9601 ggttctcgtg cagcgttttc aattactgtc tgtccttttg cttttacagc agctaacatc
     9661 acattgatcg ttgctccgac tgaaaccacg tccatgaaaa tccgattgcc acgcaatcct
     9721 ttattttctg tccgtaaata catggcacca tgttcatttg tcacttcagc acctagagct
     9781 tcaaatcctt tgacgtgtag gtcaatcggt ctagggccta aataacagcc acctggcagg
     9841 cctactaccg cttcgccaaa tttaccaagc agtgttccca taaaataata agaagcacgc
     9901 aaactattga tttttccgtg cggcatcgga atcgatacga cttctcgagg atcgatttct
     9961 agtatgttgt tggaaaaatg gacagttgcg cccatgatct ctaaaatttc aattaatgaa
    10021 tgaacatctt gtatgtccgg cacaccttcc aagatcactg gggaatcagc taagattgca
    10081 gctggaatca atgcgactgc actatttttt gcaccgctga tcgttacttc accagctaaa
    10141 ggtcggtttc ctttgattac gatttttttc atctgtttcc accttgttta ttaatctttt
    10201 ttcatggata tgcttgtatt ttatcataga taaaaaaaag aaacgagcat ggtctgccgt
    10261 tttaagtaaa aaagaatgga gagcgacttc aaaagtcgtt ctccattcct atatatcgta
    10321 tctgttatgt ggatgcttac gctttgttag ctgaaccgaa ccagtcgata tgagatttag
    10381 catcttcaac gattgctgct ttacctggag ctaataattt acgagggtcg aatcctttac
    10441 cttctttatc tttaccagct tcgatatatt tacgagtagc tgcagcaaat gataattgga
    10501 attcagtgtt aacatttact tttgagatac ccattgagat agctttttcg atttgatctt
    10561 gaggaatacc tgatccgcca tgtaatacta aaggcatgtc tggacctaca gcatcagcaa
    10621 ttgcttgtaa gtggtcaaaa gctaatcctt tccagttttc tggatattga ccatggatgt
    10681 taccgatacc gcaagctaag tagtcgatac ctgtagcaac catttgtttg cattcttcaa
    10741 tatcagctaa ttcgccgtta ccgatgattc cgtcttcttc tccgccgatt gaaccaactt
    10801 cacattcaac tgagatacct tttgcatgtg ctttttcaac aacgtcttta gctaatttca
    10861 agttttcttc aaatggtaga tgtgaaccat cgaacattac tgaagtatag cctaattcga
    10921 tacattccaa agcagcttcg taatcaccat ggtctaagtg gatagcaact ggaacagtga
    10981 tgttcattgc ttcaactaaa tcagtgatta aatctttaca gattttgtaa ccacccatgt
    11041 atttagctgc acccattgat gtttggataa gtactggtgc ttgttttgct tcagccgctt
    11101 caaggattgc ttgagtccat tccaagttgt ttgtgttgaa tccgccgaca gcgtaaccgc
    11161 ctttacgagc ag
//
LOCUS       DASJON010000094        11152 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJON010000094 DASJON010000000
VERSION     DASJON010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.094"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..347
                     /gene="metG"
                     /locus_tag="U3989_001761"
     CDS             <1..347
                     /gene="metG"
                     /locus_tag="U3989_001761"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEN1715212.1"
                     /translation="STKEKQIKFDVFEKVELKVAEVINCQKVEGADKLLQFRLDAGDS
                     QDRQILSGIAEFYPDPSELIGKKVVIVANLKPRKMRGQISQGMILSAEAPDGSLQVIE
                     APKSMPNGSEIA"
     gene            complement(826..1413)
                     /locus_tag="U3989_001762"
     CDS             complement(826..1413)
                     /locus_tag="U3989_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715213.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1588..2910
                     /locus_tag="U3989_001763"
     CDS             1588..2910
                     /locus_tag="U3989_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1715214.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3065..3841
                     /locus_tag="U3989_001764"
     CDS             3065..3841
                     /locus_tag="U3989_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1715215.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3834..4403
                     /gene="rnmV"
                     /locus_tag="U3989_001765"
     CDS             3834..4403
                     /gene="rnmV"
                     /locus_tag="U3989_001765"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN1715216.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4396..5280
                     /gene="rsmA"
                     /locus_tag="U3989_001766"
     CDS             4396..5280
                     /gene="rsmA"
                     /locus_tag="U3989_001766"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN1715217.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5731..6360
                     /locus_tag="U3989_001767"
     CDS             5731..6360
                     /locus_tag="U3989_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715218.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6484..7593)
                     /gene="ugpC"
                     /locus_tag="U3989_001768"
     CDS             complement(6484..7593)
                     /gene="ugpC"
                     /locus_tag="U3989_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN1715219.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7802..8224
                     /gene="mgsA"
                     /locus_tag="U3989_001769"
     CDS             7802..8224
                     /gene="mgsA"
                     /locus_tag="U3989_001769"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN1715220.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8547..9083
                     /locus_tag="U3989_001770"
     CDS             8547..9083
                     /locus_tag="U3989_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1715221.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(9123..10886)
                     /locus_tag="U3989_001771"
     CDS             complement(9123..10886)
                     /locus_tag="U3989_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715222.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10889..>11152)
                     /locus_tag="U3989_001772"
     CDS             complement(10889..>11152)
                     /locus_tag="U3989_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715223.1"
                     /translation="LIAKPALLILDDSLSALDYQTDLDLRKALQTYLGSTVIIISQRV
                     RSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVASQEEE"
BASE COUNT         3516 a         2028 c         2275 g         3333 t
ORIGIN      
        1 tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa ctaaaagtag
       61 cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa ttccgactag
      121 atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc
      181 caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc
      241 gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc tctttacaag
      301 taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg
      361 gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa
      421 ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta tccaaaataa
      481 atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata atactaatag
      541 gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt taattgattc
      601 atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat gactttatta
      661 catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct atcactttat
      721 gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa aacaatcatc
      781 ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact tgattttatg
      841 gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg
      901 gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat agagctttgt
      961 caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa accaatgatt
     1021 atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat aaaaatagat
     1081 cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata aaaagaaaat
     1141 cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat acaacatccc
     1201 ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa
     1261 tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta atgtgaacca
     1321 atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg ataaataatc
     1381 tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg tggaaaaggt
     1441 aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga gtttttcact
     1501 gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga aaattatacc
     1561 aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga aatcaatctg
     1621 tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt cttcaaaact
     1681 gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg gcttttgggt
     1741 ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc aatcccggaa
     1801 actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag ctttttatta
     1861 ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc gattatcttt
     1921 gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt gattgcagtg
     1981 atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg tgcttctgta
     2041 caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat atggggatta
     2101 ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa agatgttact
     2161 tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga cggctggtta
     2221 ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt
     2281 attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt ttttttgaaa
     2341 actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc atcagacgtc
     2401 atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc ggtatacggt
     2461 gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc tttagaagac
     2521 gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt tccttatgtt
     2581 cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc tttcgacttt
     2641 ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc
     2701 tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc actttatcca
     2761 atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac gatgatgacg
     2821 caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc agtctattat
     2881 caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag aaaaagtacg
     2941 aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt cttctatttt
     3001 gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa aaggagagat
     3061 aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg aagatattcc
     3121 agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga
     3181 tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata tatacagtat
     3241 tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa ataaattaca
     3301 agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg attatcattg
     3361 gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg ctatcgcaaa
     3421 agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca cgtatcaaat
     3481 cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca gtggtgatta
     3541 tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac
     3601 tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg aacgattact
     3661 tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc gaaatgaacc
     3721 aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc cgacagaaga
     3781 agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg aacatggata
     3841 agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga aggctgcggg
     3901 aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa gagatcctga
     3961 tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat cctgattttt
     4021 caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa catgcttttt
     4081 tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg
     4141 atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg
     4201 tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg gcacaagcaa
     4261 aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat
     4321 tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa
     4381 tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag aacaaaagag
     4441 attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt tctgactgaa
     4501 ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac gaacgtgatc
     4561 gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc aaaacaagtc
     4621 gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat gcaaccctat
     4681 gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac agctgttaga
     4741 gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta ctatattacg
     4801 acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact agtcgtcatg
     4861 atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc ttatggttca
     4921 ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt accaaaaaca
     4981 gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag aagagatata
     5041 ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc ggcatttcag
     5101 caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga tgaccagaca
     5161 aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg tcgtggggag
     5221 actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa
     5281 aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa taagaacagc
     5341 caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc agatgcaagc
     5401 ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa gtcacaagga
     5461 acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa
     5521 gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc acatctccta
     5581 tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt tgtatggatt
     5641 tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag tcgacattta
     5701 tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg aaaactaacg
     5761 atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc taaaatcatg
     5821 attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc gatgtttttt
     5881 acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt tttaacaacg
     5941 ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat tggggcttgg
     6001 tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt tgctaattgg
     6061 cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga aatgctagta
     6121 gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca aggatatttt
     6181 tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt tgattataat
     6241 attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt atttacggca
     6301 gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac gacatattag
     6361 acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc tctaagatga
     6421 gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct aacagggggc
     6481 aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc ctttgctgat
     6541 gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat ctacttttga
     6601 gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa gtaattcgga
     6661 aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta cttgttcact
     6721 gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac ctttttcaac
     6781 taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat ctgtgataac
     6841 accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa agccggcaac
     6901 gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt ggataaatcc
     6961 gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat cgtgagtaac
     7021 atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac gcatggctac
     7081 acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt ttgcatcccg
     7141 aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag cagctggttt
     7201 tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac gttttttgat
     7261 gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa aaacagtcat
     7321 atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt taggtgccac
     7381 gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt ccagtcccgc
     7441 gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa cgataaattc
     7501 acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat tgtcatattt
     7561 tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt cttttttagt
     7621 tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc atgttgaaca
     7681 agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat atccataaga
     7741 aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag gagtgtctct
     7801 tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga aattagcaac
     7861 agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga ctgggcagag
     7921 gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt taggcgggga
     7981 tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt ttcttcgaga
     8041 cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt tatctgatgt
     8101 ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa gaggactgga
     8161 agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc cactttcatt
     8221 ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta gctataaaag
     8281 ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata aacaataata
     8341 ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg ggaaaagatc
     8401 ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg gatgttgcct
     8461 cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat gatacgatgt
     8521 agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca cgtatgattg
     8581 ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta gcaatcgaga
     8641 ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc attctttttg
     8701 gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg gccctcttct
     8761 cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga gcaatctacg
     8821 gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct acgcttagtg
     8881 tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg taccatgtgc
     8941 cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata tggttaccga
     9001 ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa attttaagaa
     9061 gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa gactgcttgt
     9121 ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc tttttgaagc
     9181 agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa tatctggtcc
     9241 gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc ttccatcatt
     9301 gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct ggtagcttca
     9361 tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa cagttggcgt
     9421 tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt ttccggcaaa
     9481 cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc tgcgtcactt
     9541 gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag ccaagtatct
     9601 tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg gatgtcatgg
     9661 tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa attaacaagt
     9721 gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg ataagcagta
     9781 aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac atgatcaaaa
     9841 gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa atcaggcttt
     9901 tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc ggtttgcagt
     9961 tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga gtagatcgta
    10021 aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa taggccgcca
    10081 actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg gtttgtcaag
    10141 gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag cagttcaaat
    10201 tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt accgattcgt
    10261 tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa atttgtttgt
    10321 gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca aagaacgacc
    10381 aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat gattgccact
    10441 ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc catatcattt
    10501 gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag tggcaatcga
    10561 ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc taaccgattg
    10621 cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc accgaataga
    10681 ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat ttgatcgata
    10741 ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat tcctagtatc
    10801 acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg gcggagcgtc
    10861 tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg acgatttcct
    10921 gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca atcttacctt
    10981 gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg gagataataa
    11041 tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct gtttgataat
    11101 ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc aa
//
LOCUS       DASJON010000095        10955 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJON010000095 DASJON010000000
VERSION     DASJON010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.095"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..603
                     /locus_tag="U3989_001773"
     CDS             <1..603
                     /locus_tag="U3989_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEN1715224.1"
                     /translation="QRYGSQNQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALD
                     TLKSALDSIEASREKEGQALALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLE
                     EFLGAKVDEQRLLTELAILLERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQ
                     EMNREINTIGSKSNAIEIKNQVVQSKTILEKIREQVQNIE"
     gene            621..905
                     /locus_tag="U3989_001774"
     CDS             621..905
                     /locus_tag="U3989_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715225.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            1392..2006
                     /gene="gmk"
                     /locus_tag="U3989_001775"
     CDS             1392..2006
                     /gene="gmk"
                     /locus_tag="U3989_001775"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN1715226.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            2011..2316
                     /locus_tag="U3989_001776"
     CDS             2011..2316
                     /locus_tag="U3989_001776"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1715227.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            2569..4977
                     /gene="priA"
                     /locus_tag="U3989_001777"
     CDS             2569..4977
                     /gene="priA"
                     /locus_tag="U3989_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN1715228.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            4992..5483
                     /gene="def"
                     /locus_tag="U3989_001778"
     CDS             4992..5483
                     /gene="def"
                     /locus_tag="U3989_001778"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1715229.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            5476..6414
                     /locus_tag="U3989_001779"
     CDS             5476..6414
                     /locus_tag="U3989_001779"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN1715230.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            6414..7772
                     /gene="rsmB"
                     /locus_tag="U3989_001780"
     CDS             6414..7772
                     /gene="rsmB"
                     /locus_tag="U3989_001780"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN1715231.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            7785..8525
                     /locus_tag="U3989_001781"
     CDS             7785..8525
                     /locus_tag="U3989_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN1715232.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            8522..10591
                     /gene="pknB"
                     /locus_tag="U3989_001782"
     CDS             8522..10591
                     /gene="pknB"
                     /locus_tag="U3989_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN1715233.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            10756..>10955
                     /locus_tag="U3989_001783"
     CDS             10756..>10955
                     /locus_tag="U3989_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="EngC; RsgA; CpgA; circularly permuted GTPase;
                     ribosome small subunit-dependent GTPase A; has the pattern
                     G4-G1-G3 as opposed to other GTPases; interacts strongly
                     with 30S ribosome which stimulates GTPase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN1715234.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKN"
BASE COUNT         3700 a         1997 c         2441 g         2817 t
ORIGIN      
        1 caacgatacg gtagtcaaaa tcaagtgtca attagtcgga tggcggaacg cttcgtgctg
       61 gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga gcatggaaga attggcacag
      121 ttagctctag atacattgaa aagcgcactt gattctattg aggcgagtcg agaaaaagaa
      181 ggtcaagctt tagctttagt gttgaaaaag aatactgaag aattcaaaga agtactaaaa
      241 aaactcgata gttttgtaga aatctatgaa aaggaatttc aaataaggta tcaaaaaaag
      301 ctggaggaat ttcttggagc gaaagtcgat gagcaacggt tgttgacgga gctagccatc
      361 ttgttggaac gcggagatat tcacgaggaa ctggatcgtc tggcgatcca tgtacaaaaa
      421 ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg aattagattt cctgatccaa
      481 gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa atgcaatcga aattaaaaat
      541 caagtcgtcc agtctaagac gatcttagaa aaaatcagag aacaagtaca aaatattgaa
      601 tgacgaaaga ggtagagata atgaaatatt atgacgtaac gttccacgaa ttaagtggaa
      661 aaactgtggt taaacgggat atcccttctg aaaaaaacgg ctttgacgta tggaaagatg
      721 cctgtgcaga ctataatgag aacgaactgt ttattttgat caacgacggt gcttacgtga
      781 cgatgaatcg gaaatttatt gttcgaattg acacagaaga ggtagaagat ccgacagaaa
      841 aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa tactttgtcc aatatgggct
      901 tttagcaagg atgctgaaca aagaaataca ataaataaac agaattggat ttcttcgctt
      961 agcttctttg ctctaggaat acatttaaag gttgtgacaa aattcgtgtc acaatctttt
     1021 tttattttga ataagtgatg ttcaaaaacc attttctcgg tattaagcca aatttatgca
     1081 aaaatgtgag aaactatttt cacaaaattt ttcttcatac tcgaaactaa tcggcttctt
     1141 cacgacctct tatacacggc gttcaaaagc ttgcatctgc gataataacc ccaaccaaac
     1201 gaaaaacatg agaagctgtt tatgtttggc tgttcttatt acttgatgca ggacagcttt
     1261 tttacgacct cttggtacag ggtagctttt ttaccaattt ctagctataa ttaaagataa
     1321 ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa gtacaaaaag actagttgaa
     1381 aggacgtcgt catgtcagag cgtggattat taatcgtgtt gtctggtcca tccggtgtag
     1441 gaaaaggcac agtacgaaaa gcaatttttg agagtgagga taatgatttt cagtattcga
     1501 tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga aggcgttgat tatttcttcc
     1561 gaagcaaaga agagtttgaa tcaatgatcg aagctggaga gatgctggaa tacgcagaat
     1621 atgtcgggaa ttattatgga actcctttgt catatgtcaa caagacattg gatgaaggaa
     1681 aagatgtttt cttggagatt gaagtccaag gagcaaaaca agtcaaagaa aaagtgcccg
     1741 atggtgtatt catctttttg actccgccag atttagcgga attaaaatca cggatcgtag
     1801 gccgaggaac agatgcagat gaagtaatcg aaaaacgaat gaaagttgca aaagaagaaa
     1861 tcgaaatgat ggctctttac gactatgctg ttgtaaatga tgaagtgccg ctagcagttg
     1921 aacgaatcaa aaacattatt gccagtgaac attttcgagt agatcgcgta atcggtaaat
     1981 atattaaaat gttaaaggag atgtagtctt atgatgttaa aaccttcaat tgactcacta
     2041 ttagatcgtg taaattcaaa atattctcta gtgattttag caagtaaacg cgcgcatgaa
     2101 ttggatgcaa gtgcacagcc tactctagat tcttttgatt cagtaaaaag cgtcggtcaa
     2161 gctttagaag aaatcgatgc aggaaatgta gtcaatgatc cacatcctga attaaaacgt
     2221 gaacgtttga agatggaaga agaggaacgt caagcacaaa aagagcgtga acaacacgaa
     2281 ttggaatcac gtatccgtga agagcaaaaa atgtaataaa gaaaaataga gcgaacatct
     2341 aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt cgccacttat tgtctggtag
     2401 aaaaggctgt gtctgaactt tttgacacag ttcttttttt tcttatctgc tgttaatttt
     2461 ttgttaggat ttggcagaag ataagacaaa tccatcgatc tctagtaaaa taggcaagga
     2521 actttttgtc gcttgataag aaaagacaaa aaaaggagga aacgaaccat gacattaaca
     2581 gctgaagtca ttgtggacgt acctactatg cagaccgatc agccattcac ttatttggtt
     2641 ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag tagaagtccc ttttggtaat
     2701 ggcaatcgtc atgtccaagg atttgttatg gctatccaaa aatcagaaga atcagctaat
     2761 ccatctttga aggcagttat tcgtttgcta gaccttgctc cggtggtcaa tgaagaatta
     2821 ctttcattag ctgattatat gaaaaaaatt acttacgcat tcaaaatcac ttgtctacaa
     2881 acgatgcttc cgagtgtgat gaaagcagaa tatgataaat tgatctatcc tttggcagac
     2941 acaccagaag ttcaggtatt gtttgatcag cgagaagtga tttcctggaa ggaagcagaa
     3001 gaagaaggca gcctttctca attgattcgc tggcgccaag agcaattagt agatatcaaa
     3061 tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt tggtccgttc tttgttaact
     3121 gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa atgccaagaa acaaaaagaa
     3181 cttcttttgt gtttatctga actgagccaa gaagaaccaa ttgcttattt caaaaataaa
     3241 ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg gctggctgga gtttatagaa
     3301 tcagaacgtt atcgcgaccc ttataaagac cgagtatttg accaaacgac ggcattggaa
     3361 ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca cagcaggtca gcagcaaaaa
     3421 gatcaggtct ttttgctgga gggaatcact ggcagcggga aaacagaagt atatcttcag
     3481 gcaatcgctg atgttttgtc agaaaataaa acggctatta tgttagtgcc tgaaatcgca
     3541 ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg gagaatcagt tgcggtctta
     3601 catagcggac tttcccaagg tgaaaaatat gatgaatggc gcaagattga gcgggaagaa
     3661 gcgcaagtag tagtaggcgc acgatctgct atttttgccc cgctgaaaaa tatcgggctg
     3721 atcattatcg atgaagaaca tgaatcaagt tacaaacaag atgagacacc gcgttatcat
     3781 gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt gcccaatagt tctaggaagc
     3841 gcgacgccct ctttagaatc aagagcacga gctcaaaaag gcgtttatca gttattgcag
     3901 ttaaaccacc gagccaaagc agctgcacag cttccagcta ttgagatcgt agatatgcgt
     3961 gaagagtttc agaatcatcg gacctctact ttttctgcta atttgcagga aaaaatacaa
     4021 aatcgattag acaaaaaaga acaaactgtc ctactgctca atcgtcgagg atattcgtct
     4081 tttgtgatgt gtagggactg tggatttgtt cttccatgtc ctaattgcga tatctctctt
     4141 acactgcata tggatacacg ttcaatgcgc tgccattatt gcggacatga agaacctatt
     4201 cccaatcgct gtcctaattg tggcggcaat aaaatccgct actatggaac aggtactcaa
     4261 aaagtagaag aggaactccg agaactttat ccgcaagcta ggattttgcg tatggatgta
     4321 gataccactc gaagaaaagg ggcacacgaa cagattttac aaaaattcgg tgcaaaagaa
     4381 gcagatatat tattagggac acaaatgatt gctaaaggtt tggactttcc agaagtgaca
     4441 ttagttgggg tgttgaatgc tgatacctcc ctgaatttac cggattttcg ttctagtgag
     4501 catacattcc aattgcttac ccaagtttca ggacgagcag ggcgagcgga aaaagcaggc
     4561 gaagtcgtga tccaaacatt caatcctcaa cattacgcca tagaattggc taaaaaacaa
     4621 aattatgagc agttttatca gcaggaaatg catgtacggc atcgtggagg ctatcctcct
     4681 tattacttta ctgtgaaaat aaccgccagc catcctgaag aacaagtagc ggcaaagaaa
     4741 atctttcaga ttgccaatca gctaaaagaa gtgttaagcc ctcaaagctt gctattaggg
     4801 ccaacgccca gcatgatttt gagagtgaaa aaccgatatt attatcaact aatcatcaaa
     4861 tataaacatg aaccgaatct tcctcatgtt ttggatgaaa ttttgaatgg aagccagaaa
     4921 gaacagcgac aaggtctttt tgtggcaatt gacaacgaac cattaaactt tatttaagaa
     4981 agggccaaat tatgcgctat cctatattga tccaccctaa tgataaatta aaaagaactg
     5041 ctcagccaat cgatgtaatc acggacgaga caatcgcttt attagacaat ttatatgaga
     5101 caatgattgc gaatgacgga atagggatcg ctgctcctca agtaggacaa aacaagagaa
     5161 ttgctgtgat cgaagtagat gaaggcgaga aatttgaact gattaaccca gaaatcatcg
     5221 aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag tattccgcat gtctatggaa
     5281 cggtcaagcg agcagatgaa gtgaccgtgc gttattatga tcgagacgga gaagagatag
     5341 aagtgacggc ttttggctat ttagcccgtg ccttccagca tgaaatcgat cacttagacg
     5401 gtatcttgtt cattgagaaa atgatacagc agatcccgga agaagaactt gaagaatata
     5461 tggaggaaca tcttgatgac taagctagta tttatgggca cacctgcttt ttctgtgccg
     5521 attcttgaag ggctgataga atcaggttac gagatccagg cagtggtaac acagcccgac
     5581 cgtccagttg ggcggaaacg agtaattacg ccgacaccgg taaaaaaagc ggctctgaag
     5641 cacggtatcc gtgtgctgca gccagagaaa atctccggtt cgccagaaat ggaagaaatc
     5701 attgaattgg tgcctgatgt gatcgtcact gcggcatttg gccaattctt gcctgaaaag
     5761 ttattgcaag taccaaaatt aggggcaatc aatgtccatg cttctttact tcctaaatat
     5821 cggggaggtg cgcctgttca ttatgccatc atgaatgggg agaaagaaac tggtgtaacg
     5881 atcatggaaa tgatcaaaaa aatggatgca ggcggaatct atgcgcaaga aagcatacca
     5941 atcactaaac aagatgatgt agggacgatg tttgaaaagc ttagcctttt aggaagaaag
     6001 ctattgctag aaacattgcc caatattttg gatggacaaa aaccagtacc tcaagacgag
     6061 tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag aagcaatcga ttggcataaa
     6121 actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc cttggccaat tgcattcacg
     6181 acctatgagg gcatacgctg gaaattatta tcggttgaag tattggcaga aactacagac
     6241 aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc tatggattgc ttgcggtaaa
     6301 cagaccgtct tagccatcaa tgaattacag ccagctggta aaggaaaaca ggccgtacat
     6361 gatttcctta acggaagcgg tcagcaagtt cagattggtc agcaggtgaa ataatgaaaa
     6421 aaatccctaa aaaaatgaaa aaatcagtac gatatatggc attactgaca ttagagcgag
     6481 tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga gatgatcaaa caaagcgagc
     6541 taaatcaaaa agatgtgggt ttatttacag aattgatcta tggtacgatt agccatcagc
     6601 gactattgga attttacgtt gcaccattga tcgctaaagc aaaaaaagtg gatgactggg
     6661 tcaagatgct tctttatctt tctgtatacc aattagaatt tttagataaa gtccctgcac
     6721 atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa aggaaatcct gggacaggga
     6781 aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg agcaccagat ctgcaaggca
     6841 ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct gcctctatgg atgacgaaac
     6901 gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct cggtttatcg ctatatcagc
     6961 ctagccatgc tagtgcccga gtagatacac gccgactttc cagagatcag gccatcactg
     7021 ttttgcaaga agaagagatc acagctgaaa aaagccaagt cagtccatac ggtatagtag
     7081 cgaaaaaagg gcatttagct tctagctcat tgttccacga tggcgtcatg acgatccaag
     7141 atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga gcctgaacat catgtcttag
     7201 atgcctgtgc agcacccgga ggtaagacga cgcatattgc ttctttttta gatccgtcat
     7261 gcggtggaga agtggtcgct ttagatgtac acgaacacaa agtcaaactc attgaagata
     7321 atgcagcacg tctaggtgtc tcttctgctg tctacgctca aaagatggat gctagagaag
     7381 tcagtaaaca gttcgaagca gagcagtttg atcggatttt agtagacgcg ccttgttcag
     7441 gtcttgggct gcttcggcga aaaccagata ttcgttataa aaaacagccg gatgaattag
     7501 cgaatttacc aaaaatccaa ttggaaattt tagaaagctg cgcaccaact ttaaagtctt
     7561 ccggtattct gacttatagc acttgtacga tcttaaaaga agaaaaccaa gacgttgttt
     7621 ctgctttctt ggaaagacac cctgattttg agaggattga tgttttgacc aatcctatgc
     7681 tacaatcatc aatcgaagag aaaatgttga ctttgtatcc gcatcagttt tacacagatg
     7741 gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga aaaaatgcag attgaatatc
     7801 aatcagatgt aggaagacga cgtaacacaa atcaagacta tgccagtgta tttaccagcc
     7861 gttcaggtct caagcttgct gtattagcag acgggatggg tggtcatcgt gccggagaca
     7921 ttgccagtca gatggcggta acgaatctag gcgaagcttg ggaaaaacaa gaagctgttg
     7981 acgatgaaaa aatcgcccaa tggttcattc aatcgattca agaagaaaat atgctgattt
     8041 atcagcgtgg acaagaacag ccggaatata acggtatggg aacaacaatc gtagcggccg
     8101 ctctgtcaga tgactggttc acgattgcca atgttgggga tagcagagct tatttgattc
     8161 gtgaagataa aatcatgcaa ttgacggagg atcattcttt agtcaatgaa ctagtcaaat
     8221 caggagaaat cagcgaagaa atggcagtga accatccaag gaaaaatatt ttaacacgtt
     8281 ctgtcggcat gcctggaaca gtcgaagtag atgtttctac tcatctttgg caactaaaag
     8341 atcgactgct gttatgttca gatggcttga cgaacatgct gtctgaagaa gaaataggaa
     8401 cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac tgagctgatt gatcgtgcca
     8461 atgaagcagg cggagcggat aatatcaccg ttcttcttat cgaatataag gaggacagtg
     8521 tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa atcactggaa atatcggtag
     8581 cggtggtatg gcaaatgtat ttttggcgca cgatttaatc ttggatcgtg atgtagcagt
     8641 taaagtctta cgatttgact ttcaaaatga ccagactgcg attcgacgat ttcagagaga
     8701 agctttagca gcaacagaac tcgttcatcc aaacattgtt agtgtttacg atgttggtga
     8761 agaagacaac atgcagtatc tggtgatgga atacgtcaaa ggaatggact tgaaacgata
     8821 tatccaaaca cattatcctg ttccttatga aacagcagta aatattatgc agcagatcct
     8881 ttctgctatt tctttagctc ataaccatca aatcatccac cgagatttga aaccccaaaa
     8941 cgtactgatc gataatgaag gtgttgtaaa aatcacggat ttcgggattg ctatcgcttt
     9001 atctgaaaca tcgatcacgc agacgaatac gatgctagga tctgttcatt atctgtcccc
     9061 tgagcaggcc agaggaagta tggcaacgaa gcagtcagat atttatgctt tagggatcat
     9121 cctttatgag atgttgacgg gtagcgtacc gtttgatggt gaatctgctg taacaattgc
     9181 tttaaaacat tttcaagatg atcttccatc tataaaagca cttgatccaa atgtgccaca
     9241 agcactggaa aacgtgattt tacgggcaac ggctaaagaa cctgcagacc gttataagtc
     9301 agccgaggaa atgtctgatg atctttctac agtattatca ccagctcgtg caaacgaaga
     9361 aaaatggcag cctcatgtga tggataatga aacaaaagtt atcacacccc ttacggaaga
     9421 tgcaccaatg cctgattcat tcaaatctat gcctctgcca aaagacaagc cagaaaacga
     9481 aaccttacca gaggaggaag aagttctgcc tgatcctaaa aagaaaggac agaaaaaatg
     9541 gtggctgatc ctattagcta ttcttgctgt tttaggaatc ggaacagctg tattctttgc
     9601 ttctggcggg cgaggagaag ttgttgttcc tgatgtttct gatctttctg aagcttctgc
     9661 tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca aaaacagaag aaattgcaga
     9721 tgacaaaatc gaagaaggta atgtagtgaa gacagacccg gcagcgggaa cgacagtcaa
     9781 gcaaaaacgc gaagtcactc tgtatattag tagcggcacg aaaaaaatca aattagatga
     9841 ttacagtggt aaaagttatg aagatgccga agcaagtttg aaagaattag ggttttctgc
     9901 tgacttgatt caaaaagaag aggaattcag ttcagaggta gaaagcggaa tgatcatttc
     9961 gcaatctcca tcagaagata cagaagtcga tcctaaaaca gacacgatta ctttcacagt
    10021 cagcaaagga ccagaaacag tgactttgag tagtcatgta ggagaaaact acgaaactgt
    10081 tcgtaattgg cttgttacac aaggattcaa tatcaataat attagtgcta cttatgacta
    10141 tagcgacact gtaccatcag gttcgatcat cagtcaggat ccagcctcgg gtgaagttgt
    10201 agcagaagaa acttacatca attttgtcgt aagccaagga acagagccta aattgaatga
    10261 tatctccggc tatacaaaaa gcgaagcaca aagttattta gctagtgtcg gtgcagaata
    10321 tatcgggcat gagacttccg aatattcaaa cacggtggat aaagataaag tgattcgtac
    10381 agtaccaggg gcaggaacga caattaccaa aggaatggtt gtgaacgtaa tttactctaa
    10441 aggaccagat ccagcgtctt ctagttctga aaaagactct tcagacagtt ctagcagctc
    10501 aagtagctcc agtagcacaa gcaactcttc aaaagaagaa tcttccagct ccacaagttc
    10561 atcagctagc acctcagaaa gttcaaaata agtgctgaat aaaatatatc agaagggaat
    10621 gcgacattga tttgttgcat tctctttttt gtgtttctat ctcttttgat caattttcaa
    10681 gccatatact ttgagaagtt tgcatggaat aacacctact ttcctcttcg tatggtaaaa
    10741 taaacaaaaa cggaggtggc acttatcaaa ggacaaatca gaaaagcaat cagcggtttt
    10801 tactatgtat acgcaaacgg agagacctat caaacaagag gaagaggaaa tttccggaat
    10861 cggaaaatca cgccgcttgt cggagatcag gtgatctttg agagtgaaaa tcaaacagac
    10921 ggctatcttc tggaaatctt gccgagaaaa aatca
//
LOCUS       DASJON010000096        10855 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJON010000096 DASJON010000000
VERSION     DASJON010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.096"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1620)
                     /gene="metG"
                     /locus_tag="U3989_001784"
     CDS             complement(<1..1620)
                     /gene="metG"
                     /locus_tag="U3989_001784"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN1715235.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQT"
     gene            1888..2166
                     /locus_tag="U3989_001785"
     CDS             1888..2166
                     /locus_tag="U3989_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715236.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2263..3084
                     /locus_tag="U3989_001786"
     CDS             2263..3084
                     /locus_tag="U3989_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1715237.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3369..4379)
                     /locus_tag="U3989_001787"
     CDS             complement(3369..4379)
                     /locus_tag="U3989_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1715238.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4391..5344)
                     /locus_tag="U3989_001788"
     CDS             complement(4391..5344)
                     /locus_tag="U3989_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1715239.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5334..6404)
                     /locus_tag="U3989_001789"
     CDS             complement(5334..6404)
                     /locus_tag="U3989_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715240.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6410..7453)
                     /locus_tag="U3989_001790"
     CDS             complement(6410..7453)
                     /locus_tag="U3989_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1715241.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7454..8395)
                     /locus_tag="U3989_001791"
     CDS             complement(7454..8395)
                     /locus_tag="U3989_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1715242.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8479..8826
                     /locus_tag="U3989_001792"
     CDS             8479..8826
                     /locus_tag="U3989_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN1715243.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(8896..10557)
                     /locus_tag="U3989_001793"
     CDS             complement(8896..10557)
                     /locus_tag="U3989_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1715244.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3091 a         2204 c         1972 g         3588 t
ORIGIN      
        1 tgtttgagta ccttcagaca ttttttcttg aataaaagca acttcttctt ccatatctaa
       61 acgtgggaag attggtgtac cttttgcaac tacttttgtt cctgaaggaa attcgccaaa
      121 gtgtaatcct tcaagattca ttgtttcaga gtccaatcct agttgattga agatcttctc
      181 tggcgtttct gtcatgattg gctgaagtaa tatcgctgcg atacgcaaag attcagctaa
      241 atggatcatg acactattca attcagcttt cttttcttcg tcttttgcta atacccatgg
      301 ttctgtttca tcaatgtatt tgtttgcacg agaaatcagc acccagattt cagccaatgc
      361 tgtattgaat tccatttttt ccattgcatc atggtaacgt ccgacaacgt tagcagccgt
      421 cgtagacagt tcactatcaa atggagtgac aagtgaggca tagtcaggaa cgagtccatc
      481 acaatatttg ttgatcatag caatcgttcg attcaataag ttccctagat catttgccag
      541 atcataattg acacgggaaa caaaatcttc tggcgtaaag acaccatctg acccaaacgg
      601 aacagcacga agcaaataat aacggagtgc atctaaacca taacgttcca caagcatttc
      661 aggataaacg acatttcctt ttgatttaga catttttccg tctttcatca atagccagcc
      721 atgcccaaat acttttttag gtagaggcaa atcaagggcc atcagcatga ttggccaata
      781 gatcgtatga aagcgaacga tttctttacc gaccatctga acatctgcag gccagtattt
      841 ttcgaataga ctttcatctt cggagccata tcctagtgca gtaatataat tggataatgc
      901 atcgatccat acatacacca catgtttcgg atcactcttg actggtactc cccaagtaaa
      961 agttgttcgt gaaactgcaa gatcctctaa tcctggtttg ataaagttgt tgatcatttc
     1021 gtttttacga gattctggct ggatgaattc gggatgttct tcatagtatt ccaatagacg
     1081 gtctgcgtat ttgctcatgc ggaagaagta tgattcttct tttaccaatt ctacttcgtg
     1141 tccacttggc gctttaccac cgatcacgtt accttcgtca tctttgtaca cttctgcaag
     1201 ttgtgtttct gtaaagaatt cttcgtcaga aacagagtac cagccttcat attcacctaa
     1261 gtaaatatct ccctgttcta ataaacgatc aaatattttt tgaacagctg ctttatggta
     1321 atcatctgtc gtgcgaatga atttatcata actgatatca agcgttttcc ataatttctt
     1381 gacatcagcc gccattttat ctacatattc ttgcggtttt acacctagtt cttctgcttt
     1441 tgtttcgatt ttttgaccat gttcatccac tccagtgaga taaaacacat caaagcccat
     1501 caaccgttta taacgagcaa ttgcgtcaca tgcaatcgta gtatacgagt tgccgatatg
     1561 aagtttgccg cttggataat agattggcgt agtgatataa aaagtttcct tttctgccac
     1621 ggggctcttc ctcctttatc gcatgatgcg aattcgtttc cttgctagta taccataaaa
     1681 aaagcatgct gttagctttt tttcaaaatg aaaaagactg gataaacgac ttctatctac
     1741 gttcaatcta tcctttaaaa ggatgaatag gttgaataaa tgcgggtttt tgttttgttt
     1801 catatctact aaaaaacggc tttttatttc tcccctcaca cgtggtataa taaagactat
     1861 ctatcttagt aagggactgg aacaatcatg cagaagaaac atcttttttt tacacttagt
     1921 attgcttttc tttcattagc acatttaata ttcagctatt tttatattcg tatgtatggt
     1981 tattttaacc tccatgggca tttgaacagt tttatgacag cagcttggat tttgcgtttt
     2041 atcattgacg tatatatcgt tatttgtggc ttctttgcca ttcgtgaaga gcggtataaa
     2101 gtattgccgt tttatctgct tttcttttta ttcaatctca tacttccatt tatttttcat
     2161 atttaaatga taacctagat ttttcaagta aatccttggt tttttttcag agtctgctac
     2221 aataaggtaa aaagtacttt tactgtcaat ggaggtggtc agatgaaccg ttttgcttct
     2281 aaagaagaag aaaaattata ttatgaacat aaagcaagtg aagaagaatt tcttaactgg
     2341 tatcaaaaac aggaacgtcc agaatatgac aagccctctt tgactgtcga tatcgtactg
     2401 atgtgctata acaaagaaga agatcagctg aaagttttgt tgatccaaag aaaagggcat
     2461 ccttatcgaa attcatgggc attgcctggt ggctttgtcc aaaaggatga gtcaactgga
     2521 gaaagcgtct taagagaaac aaaggaagaa actggtgtgg ttatttcaaa agaaaatatt
     2581 gagcaactgc atacattcag tacgcctaat cgagatccgc gaggctgggt cgtcacagtc
     2641 agctacttag cctttattgg agaagaaccg ctaatcgccg gagatgatgc caaggaagta
     2701 cgctggttca cactagaacg tcatagtaac aagatccatt tatctagcgg agaagtggct
     2761 atctcgcttg atttagtcac tggagagtct tctggcaaag atactttggc ttttgaccat
     2821 agtcaaatca tcttgaaagc attcaaccgt gtagtcaata aaatggaaca cgagccgcaa
     2881 gttctacaag ttttaggtaa agatttcacg attacggaag ctcgaaaagt atttgctaaa
     2941 ttcttaggga tcgattataa aacgatcgat cattccaatt ttaagaaagc tatgctccaa
     3001 tatttcgatg agatcggtga acgtcctgta ggaatcggcc gtccttcaaa aatctatcag
     3061 ctcaagccgg ggcaccatga ataaggttgt gagcaaaaga ttcagaccta atcattcaag
     3121 aaaaaacgca aaaacaactc tttgtgagtt acgttttttg tttcagaatt ttgatgtatt
     3181 cgtaaacttt ctgaataaaa aattaacaat agcagaaaaa gctcgtacga actttttctg
     3241 ctattgtttt attggtttgt cataattagt gagcttttct tcaaaagaaa aattcctaga
     3301 aacaaaagtc attaagactt tttattaaca aggctaaccc taacgctcct atcagtttca
     3361 ttcatttttt atcccttgat aaccgaatgt ctgcccattt tttctactac ttggctcttt
     3421 cttacggaat tgctttcttc tgctccgtcg gtattgatca aaagaaattt tttattatac
     3481 cattgggact tataacgcaa ttttgttatt aatttatgcc agtcgtgaat agaaaaatca
     3541 gcatgtgcct cttcttttgg aaaagattct acaaataagt agtcatattg tttgaccaat
     3601 ttcccagata attgttcaag ataatcttct cgctggttct tttgtttcag ataaagatcc
     3661 attacttttc tcttttgttt ctgataattg cttgcctgat ctaacctgat ctttctatgg
     3721 tgagcggagc ggactttcac attcaatttc cgctgtgcat gttgcagctt ttcttctgtc
     3781 tggtgaaatt ttggtaaagt tacttctaga ggtcgagatg tttggatcaa tgtcttcgga
     3841 tgataggcga tcccgatcca tttagctaaa gattcttttt ttactggcga aacatcacaa
     3901 agtagtgcga cataaaattc ttcattatat cgagcagaaa tcgtagcgga gcgaatcgat
     3961 ccttcaactg gccgatgctg atttatcttg attagttctt tttgttttgg taactttaag
     4021 tagccatttt ccagatagat cgtacgggtt tggttgttgg tcgtataaga ttgccacata
     4081 ctttttttcg ttttaagctt tgggaagctg gcacgtccct tgaaataatt gcgaaaagcc
     4141 cgatccaaat taagctgtgc atttgctaaa gcaagactgt ctacttcttt taagaatggg
     4201 tattcttttt ttaatgtagc gggtgtgagc gaaaaattgg ctactcctgt ttgttgccaa
     4261 cttttctgcc gctttttcag caaatggtta taggtaaatc gagcacatcc aaaagtacga
     4321 atcaattgct gtttttgttc ttctgtcgga tacatacgga atttaaaagc ttttagcatc
     4381 tatgtccctc ctagttagca accagaagag attccttttg tcgttttagt ttttttgcgt
     4441 aatatgggac ttcctgctca gttgcataga caaaatgttc tggtgcgatt tcacgaagtt
     4501 gacgtacttg tccgtcatct ggttcaggtt gatatttgat tcttctgcgt tttctctcat
     4561 ggtctggatc tggtagagga atagcagata acaaactttc tgtataagga tgaacaccgt
     4621 gattatagat ttcatcactt gttcccatct ctaataacaa gccgttatgc attacaccga
     4681 ttcggtcact aatatgttta accatcgaca aatcatgtgc aataaataga taggtcaatt
     4741 gatgttcttt ttggagatct tgcaacaaat tgaccacttg ggcttggatc gacacatcta
     4801 gtgcagaaat tggttcatca caaatgataa aacgcggttt tactgccaat gctcgtgcaa
     4861 taccaatacg ctggcgttgc ccacctgaaa attcatgagg ataacgagta ccgtggcttg
     4921 gattcaatcc aacagttctc aatagatcat cgactttttt tgcacgctct tcagatgatt
     4981 tagctagtcc attaacatca attccttcgg caatgatatc tctgactttc atacgaggat
     5041 tcaacgaagc gtatggatct tgaaagatca tctgaacatc atgccggaat ttcgtcaaac
     5101 cttttttatc tttgatcttc gttatatctt gaccgtcgaa taatatctca ccatcagtag
     5161 gttcattcaa gcgaatgatc gtacggccag tagtggattt tccgcttcca gattctccaa
     5221 ctaaaccgaa tgtttctcct tcataaatgt gaaagctgat gtcatttaca gcatgtactt
     5281 cattcttttt tcctacattg aaatactgtt tcaatccttt aacttcaagt agtttatttg
     5341 gcatcagcaa ttcctccttg accgtaagta tgtttttgtt tctctttaaa gatagcccag
     5401 cgacgttgga tttcttctgg cggtgtcact ttgggtgcct gcggagcaag tagccaagtt
     5461 gctgccttgt gtgtttttga tagttcaaaa aatggtggtt cttgttctgc atcgattttc
     5521 atcgcgaatt catttcttgg atagaatgcg tctccttttg gcgggttcaa taaatccggt
     5581 ggagaaccag gaatagcata caatcgatcg ttcgcactat ctaatgtcgg cattgaacca
     5641 agcagtcccc aagtataggg atgttgcggg ttgtagaaaa tttcttccga tgttccaact
     5701 tcaacgattc ttcctccata catgacagcg acacgatcag cgacattcgc tactacacca
     5761 aggtcatgag tgataaagat aattgatgta tcgatttttg tctgaatttc tttcagcagc
     5821 tccaaaatct gtgcttgaat agtcacatca agcgctgttg taggttcatc agcaatcagt
     5881 atttccggat aacataccag tgcaatcgcg atcacgatcc gttgacgctg acctcctgag
     5941 aactgatgcg gataactgtt gatacgtttc tcagcttcgg gtattccaac taatttaagc
     6001 aattctaatg cagctttttg tccctctttt tttgaaactt tattatgctt tctaagggac
     6061 tcagcgactt gcttcccaat gggcatcgtc ggatctaaag aggtcattgg atcttggaaa
     6121 atcatcgcta tttctttacc acggattttt tgcatctctc tttctgattt tccaacgata
     6181 tcgttacctt taaagaggat ttgaccagca tctatattgg cgttgcttga cagcaagcgc
     6241 atgatgcttc tggtcgtcac tgattttcca cttccagatt ccccaacgat cgccaatgtt
     6301 tctcctttat tcaaatgaaa attgacaccg cgaatcgctc gtacctttcc agcatacgta
     6361 tcaaaagaga tttctaagtc tttcacttct aaaattttcg tcatcgtctt cactctttca
     6421 ttttaggatc aaaggcatca cgcaatccat cagcaagtaa gttaaagcca atcatgataa
     6481 ccgataaagt tactgccgga atccataata gataaggcag atacaggaat gtcttgtacc
     6541 cttcgctcaa catcgttcct aatgaagctg acggaggcgt aagacctaaa ccgataaagc
     6601 ttaaaaaggc ttcgaagaaa atagcagatg gaatactaaa catcatttga ataataatga
     6661 cactagagat attcggcaaa atatgtttga atgcaatttt cattcttgat tcacctaatg
     6721 tcaatccagc taaaacatat tcttgatctt ttaatttcat tgtttgtgca cgaacgatac
     6781 gtgccattgg tatccagttt gtgatggcca tagcagccac gattgaaaaa attcctggtt
     6841 caaaaacgac caacatcaaa atcatgacaa cgaggtttgg aattcctgat aagatttcca
     6901 aaaaacgttg catcacgtta tcgacacgtc cgcctaacaa accagaaacc aacccgtaag
     6961 ctacaccaat cgttacatct agtaatgcag caataaatgc gatcagcaac gagattctca
     7021 ttcccataaa taagcggctt aatacatcac gtcccaaacc gtctgttcct aaaaagaaat
     7081 ttacgtttga agggacgttt gcttgagcat atttatcaac aagttcacca ccaaccattg
     7141 cttttccatt caatccatcg atattgattc ccggaatacg aggaggcaaa ttgatataag
     7201 ccacgttttg ggcagttgga tcatgaggag aaacaaagat tgtgatcaca gagatcaaaa
     7261 tgatgaccaa tagtaaaacc attgaaataa cggcagcttt gtttttcttt aaacggcgcc
     7321 atgaatcttg gataaaattc aaagaaggtg cagtaatctg ttcacgttct tcttcggtat
     7381 ttttttgtaa tggaagaaat tcatccgcag ggatatctgc aactgtttca taacgctttg
     7441 gtatcatttc catttatcct cggctccctt ctgaaacacg aattcttgga tcaactagac
     7501 cgtacaatag atccacgatc aaaataacaa aaatcagcat aaatgagtaa aggatcgtta
     7561 cagccatgat cgtaggataa tcatttgttg tgattgactt aacgaattgt tccccgatac
     7621 caggaatagc aaagatattt tctaccacca gtgatccggt catcaatgct acagctaacg
     7681 gtcctaataa agtcatcaat ggaatcaaac tatttcgaag accatgttta aaggcgattt
     7741 cccaacggct caatcctttt gcttttgcta attccacata atcactatgc agtacttcca
     7801 ccatttccgt acggataaac cgagcagagt cagcaagcgg cgacatggcc aacgcaattg
     7861 ttggtaagat tgtgtacaca aagccatccc attttgcgat tggcaaaact tgtaatttga
     7921 ttgcaaagat atattgtaat aaaacagcaa atacaaagtt tggaatggaa cgacctaata
     7981 tcgctacgag tgtagaagat gtatctgccc atgtattttg cttcatggca gagatcgttc
     8041 ctaaaatcgt ccctaggact gttccaaaga taattgcctg taaccctaac tgcaaggatg
     8101 gtccgattct tccagctaac aaatgtgcga ctggctggtt tttaaattgg aaggaaattc
     8161 caaaatctcc ttgaagcaaa ttagaaagat aaatcccata ttgtacgata actggtttat
     8221 ccaaaccgac ttgtttattt agcatctcga ttgtttcagg actcaaacgt tcctgattcg
     8281 tatatggcgt gcccggcagc aattgcatca agaaaaaggt gattgttgca attaaccaaa
     8341 gcgtgatgat catgaaaaag acacgtttta atacatattt gatataacta ttcactaatg
     8401 tttcccccta gcttttgact tctgacagat gtttcgctac attattagat aatgaaccaa
     8461 aaataaagga gcaatcaaat gaaaaaaaaa tctatcccct acgcagtagc ttttctactg
     8521 attctggtta ttttaataaa aaatctcatc aaccattcct ttacactcat tcaattatct
     8581 aatgatctat tcttatggtc attgccgttt ctgattatcg gcggtttttt atgggtcttt
     8641 tcttcgggtt tcttcgatca ttttcagcga tcggtccatc ttgcacgaac aaggaaccga
     8701 aagaaaaaac cagaattttc ctcgctctcg tctgccagct acggtatgta cagcttttgg
     8761 ctgatcattg ccggaatact aattgctctt tctgctattt tcatgctttt ttctttgtta
     8821 ggataagtga atatgagaac gaggttgcga catttgccgc agcctcgttt tttctcatgc
     8881 tcacggtatt aacgattatt cttcaacgta tgtccatttg taatcccatg aagcgcctgc
     8941 cgcatggtga acgatacctt tgattttttc attcactagg tgaccttctg ctttttggta
     9001 caccggaacg acacccatat catcagagat gatcttgatt gctttttgga aatcatccca
     9061 acgggcttgt tcatcgccag cgtctgttga tgaagctgct ttaactgctt cgtcatattc
     9121 tgcattgctc cagcgaccac ggttgtatga attacctgtg acaaacaaat cagtaaagct
     9181 gcttgggtct gcatagtcag cagcccatcc gcctagtact gcgtcaaaat cacccgaagt
     9241 tgagcggtca agacgtacag agaatggaac aggtgttggt ttgactgtta cgccatccaa
     9301 gttttcttgg attgagtttt gaacatattc gattactttt ttcgatgaat catcatcaga
     9361 agccatcaaa tcaaattcta aagaatcaac gcccaattct tttttcgctt tttcccaata
     9421 ttcttttgct ttatctttgt cataagaaac taattttcca gcttcttttg caaagtcttc
     9481 gttcgttgtt ggacttttta ccatatctga tgggatcaat cctgtagaga caactgatcc
     9541 gtcacctagt acttgtttta ctaaagcttc acgatcgata gaataagaaa tagcttttct
     9601 taggttcaca ttgttgaatg gtgaattctt ttcacgttga ttgaattcga tatagcttgt
     9661 ccgcgcttct ttaacagaag tataagctgg atcgtttgcg ttttgctgtg ctaactcacc
     9721 agacaagatc acgtcatctg cttggccatc tttgaataag ttcaatgcag tagaagattc
     9781 ttttactaca ctgactttga tttcagaaag ttttactgta tctttgtccc agtaatgatc
     9841 atttttctta taagtccatt ctgtatctgt tcctggtcca tcaaaatctt ctaatgtgaa
     9901 tggtccatta taaacagctt tatcacttgc agatgcatag gcatccccat tttcttccac
     9961 tacggattgt ttttgtggga aaaatgatgg gaaagcaagc agatattgga aatatggtgt
    10021 ttgtttcgtc aatttgattt ctaattcata gtcaccaact gctttgatcc ctagttctga
    10081 tttatctttc tttccagctg taatgtcttc tgcattttca actggcgcat acaggtaagc
    10141 atattcagaa gctgtagctg ggtctactgt tcgttgccag ccataaacgt aatccgctgc
    10201 tgtaacagga tcaccatttg accattttgc gtcttctcgt aatttgattt tgtaagttaa
    10261 tccgtcttca ctgacttctg ccaactcgct agctcctgcc ggctgtggtt tgctgtcttc
    10321 atcaagacgg taaattcctt cataaacgtt attcaatgct gtaaaactga ttgtatctgt
    10381 tgcaactgat aagtcagctg taggcatttc ttgaggcacg actaagttaa atacgcctcc
    10441 tccatctgct gaaccgcttt ttgctgaact actgcttgtg gctgaactgc cgccataaca
    10501 tccagcaagt acaaggccgc ataccgcaac caaaccaaat gtaaatttct ttttcatttt
    10561 acgtacccct ttattggaat tttcagaata attaatattt tctgaagttg tttaagagtt
    10621 taatgaataa ttattaaaaa atcaacactt ttttttgtct ttttcataaa ataatgattt
    10681 tttcgtgagg tttcaaggaa tttttcagaa aaaatagctt tgtacaaggt tttccatggt
    10741 cttccaaaag ccattaaata attttcgtta tattgtctat tattgccata caaagataaa
    10801 aaagaataaa caaaaagaaa ctgtgacaaa agtcttgtca cagtccctta tctga
//
LOCUS       DASJON010000097        10789 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJON010000097 DASJON010000000
VERSION     DASJON010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.097"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            189..1448
                     /gene="lysA"
                     /locus_tag="U3989_001794"
     CDS             189..1448
                     /gene="lysA"
                     /locus_tag="U3989_001794"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN1715245.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1435..2235
                     /gene="dapF"
                     /locus_tag="U3989_001795"
     CDS             1435..2235
                     /gene="dapF"
                     /locus_tag="U3989_001795"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN1715246.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2238..3119
                     /locus_tag="U3989_001796"
     CDS             2238..3119
                     /locus_tag="U3989_001796"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN1715247.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3116..3832
                     /locus_tag="U3989_001797"
     CDS             3116..3832
                     /locus_tag="U3989_001797"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN1715248.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3838..4533
                     /gene="dapD"
                     /locus_tag="U3989_001798"
     CDS             3838..4533
                     /gene="dapD"
                     /locus_tag="U3989_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN1715249.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4549..5550
                     /locus_tag="U3989_001799"
     CDS             4549..5550
                     /locus_tag="U3989_001799"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN1715250.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5547..6908
                     /locus_tag="U3989_001800"
     CDS             5547..6908
                     /locus_tag="U3989_001800"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN1715251.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6868..8019
                     /locus_tag="U3989_001801"
     CDS             6868..8019
                     /locus_tag="U3989_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN1715252.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7994..9163
                     /locus_tag="U3989_001802"
     CDS             7994..9163
                     /locus_tag="U3989_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1715253.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9338..10717
                     /locus_tag="U3989_001803"
     CDS             9338..10717
                     /locus_tag="U3989_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1715254.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3589 a         1881 c         2412 g         2907 t
ORIGIN      
        1 ataaatagct tctaatagtt cgggtatttt gtttttataa ccatttttat tcttattttt
       61 aaaaaaatat ttcgaaatat actagacaat tcagaaaatt ttgtttaata ttattccaat
      121 aaaattcaaa tctttatcaa atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga
      181 atgaaaaaat gacagaattg ataattggcg gagtcacagc aagcacattg gcagaaaagt
      241 ataagacacc tctgtatgtg tatgatgaag aaaagatgag acaaacgatg ctgtcattca
      301 agcgaggctt tgtatcgaaa cgttttgata caaaagtgct ttacgcctct aaagcattcc
      361 aaacgattga gatgctgcat ttagttcgtg aatatggatt cgggctagat gttgtcagtg
      421 gtggggaaat ctataccgct ttgcaagctg attttcctag agatactatc tacttccatg
      481 gtaacaacaa gacgccagag gaactgatct atgcacttga aaatgatttg ttgcatattg
      541 ttgccgataa tttgatggaa gttgaattat tagcagaatt aagccaacgc tatttaaaaa
      601 aaatctgtat catgcttcgg ttgaatgtcg gagtagaagc acatactcat gaatatatcg
      661 tcactgcgca tattgattca aaattcggca tgagctatga aagtaaagag tgccaacaag
      721 cccttcagtt gataaaagaa agcagctatc tagagttaga aggatttcat gctcatatcg
      781 ggtcacaaat ttttgatgtt actgcttggt tagctgaaat cgacaaattg acgaattact
      841 taaccgattt tgaccaacct ctttccttga atcttggcgg agggtttggg attcgttata
      901 cacaaaatga tcagccgatc ccagtcgaag aggcactaaa aaaactgatt gaatatacgg
      961 aagaagcttt ggaaaaacga aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa
     1021 gtattgttgg agaagctggt acaacactat acacgattgg tttcatcaaa aagacaccac
     1081 ataaaaaata ttactttgta gatggcggga tgacagataa tatccgtcca gctctgtatc
     1141 aggcagaata cgattgtgat ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga
     1201 cgattgcagg aaaaatgtgt gaatcaggcg atgtggtaat taaagaagct tatttgccga
     1261 aagcagaatc gggtgatctt ttagctgttt attccacggg agcttatgga tactcgatga
     1321 gcagcaacta caatcgagcg acacgaccag cagttgtttt tgtcaataat ggccaatcaa
     1381 gactgattgt tcgcagacaa gcattttctg atctactaag gggggaagtt tcctatgatc
     1441 cttcataaat ataatggctg cggaaatgat tttatcttat tagattacgc agaagacaca
     1501 gattatagtc aattagccgc ttctttatgc acaaaagaaa aatatgatat agacggattg
     1561 atcgccgtaa aaatcgatcc gttggaaatg atctattaca acaaagatgg cagccgcgcg
     1621 ccgatgtgcg gaaatggcat tcgctgtttt gctcgatacg taaatgatca gaacatgatg
     1681 aatgaacagg aattcaacgt agagacatta gcaggcacaa gaaaaattac ggtgttggat
     1741 ccctcacctt tttattgcag cgtagatatg ggagtgccag atgattctcc ggaaaaaatc
     1801 aaagctaatc aaacttatcc aataaaaaac caggaaatag agatcaatgg tgaaaaagta
     1861 acgatcacta gtttattttt aggaacgatt cataccgttg tttttgttga aaatgcgcgt
     1921 gaggaagtct ggagaaaaag aggtgaactg atctgtcatc atcctttgtt caaagaaaaa
     1981 accaatgtca actttgtaga agtagtcaat gaacaagaat tgatcgtccg tacctatgaa
     2041 agaggagtag gttggacact tgcttgcggt acaggttgct gtgcctctta tgtggttgct
     2101 aaggaaactg ggaaaatttc agcagatcag gtagttgttc acttggaaca aggagacttg
     2161 atgatctctg gtgaggatgc gatacaaatg gctggcccag cagttcatga atggacaaga
     2221 atattggagg aatgaagatg ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg
     2281 aagagggatc agtagattat gctggtttgg aaaaattgat ccaattccat ttagatgaac
     2341 aaacagatgg attattagtt ttaggtacaa caggagaatc ttctacgctc acacaatctg
     2401 aggaggagca gatccttcaa ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg
     2461 caggagctgg aacgaataat acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt
     2521 tgggggcaga cgcacttttg gtcatcaccc cttactacaa caaaacaagt gatgcaggac
     2581 tagctgctca ttttacagca attgcagaag cctcaccgat tccgcttatt ttgtataatg
     2641 ttccttctag aacaggcatg tcgattccaa tccatgtatt agtaaattta gcagaacatc
     2701 ctaatatcat tggtttgaaa gaagcctcag gagatatggc ttatgtgatg gatgccgctc
     2761 ggttgattgg agaagaattc ttcctttaca gtggaaatga tgatttgatc ctaccagtaa
     2821 tgagtgtagg aggaagcggt gtaataagtg tatgggcgaa tatccaacct aaaatagtcc
     2881 atgaattagt aaaagatacg caggacggaa gatggcagca agcaaaagaa aagcaattaa
     2941 atgcacttga actgatccat gccttgttca gtgagaccaa tcctattcct gtcaaagcag
     3001 ccatgtcatt attagactta ccttccggac ctctacgcct gcctttagtt tctctttcaa
     3061 aagagaaaaa gaaacaatta gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa
     3121 aatcggactg attggcagtg gacagatggg aaatagagtc gaagaagttg cacaagaaag
     3181 aggagatacg gtccttcttt tacggacggc acctaaagaa gcaactacgc ttgcttttac
     3241 ggaactgcca gaactgctta ttgatttttc tcatccagat aatttagaga aaatcctttc
     3301 gtatagctta tcatatcaac ttccgttagt cattggcacg acaggttata cacaatcaca
     3361 gttccaagaa ataaaagaac atgccaaaca tgtacctgtg atgtatagtg ctaatttttc
     3421 gttcggcatc atggtgatga atcaactaat caagcaagca gctgcaatgt tgcaaggctg
     3481 gcagatcgaa ctgattgaaa aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc
     3541 aaatatgctg cttcaaacaa tccaagaagt gcaaaagctg cagcccgtat acaactggca
     3601 agggaaaaaa agagaagaaa accaaattgg tgtacacagt attcgtgcag gttctttacc
     3661 tggtgaacat gatgtattgt ttgctgctac tgatgaaata tttacgatca aacatgaatc
     3721 tttttctaat cgaatctttg ctgcaggcgc agtagaagca gcggactggt tgaaagatca
     3781 gtcaccaggt tattacaaat tagaagattt attaatggac aaaggggtgt aaaaaacgtg
     3841 gaagctcaag aaatcatccg ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc
     3901 tatctgaatg taaccgagcc aatcgaattt aaagaaagta aagtttttgg tgaaggaagc
     3961 agcttcgttg tttttggtga ctatgagacg attgctccgg ttttagaagc tgaatcagaa
     4021 aaaatcattg aaatcgaaat cgaaactgct gcaagataca gcgcagttcc tttgctagat
     4081 attaaaaaaa ttaatgctcg tatcgaacca ggtgctatta tcagagatca agtgtcaatc
     4141 ggcaacaatg ccgtgatcat gatgggagcg attatcaata ttggtgcagt aatcggtgaa
     4201 aacacgatga ttgatatggg tgctgtctta ggtggacgag cgacagtggg caaaaactgc
     4261 catattggtg caggagctgt cttagcaggt gtgatcgaac cagcatcggc caaaccggtt
     4321 atcgtagaag acggcgtact ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc
     4381 ggaaaagatg cagtcgtggc agcaggagca gtggttttag aagatgtggc agcagaaact
     4441 gttgtcggtg gaatacctgc acgtgtatta aaaataagtg atgatcagac aaaagaaaat
     4501 acagcattga ttgctgcgtt acgcgaatta tagaggagat ggagaaaaat gcgcgaaata
     4561 gatacctgta ttgtgggggc aaccggatta gtcggccaaa cgatgctcaa agtgttagaa
     4621 gaatatgatt ttccagtagg acgattgaaa ttattagctt caaaacgttc agccggtaaa
     4681 gtttgtgtat tcaagaaaaa gaaacacact gtacaggaat tgacagaaac ttcatttgaa
     4741 ggatatgatt tagcgctatt ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc
     4801 gcacgaagtc agggactatt tgtcatagat aattcctcag catggcggga agattcggat
     4861 attccattaa tcgttccaga ggtcaatctg gaagactata cattgaatcg gctgattgca
     4921 aatccaaatt gttcaaccat tcaagcggta cttccgctta aagctttaca agaagccttt
     4981 ggtatccagc gagtcaatta ttcgacctat caagcagttt ctggttccgg gcaaaaaggc
     5041 attgaagatc ttcatcgtac gcaaaatgac gaggaaccag ttttgtatcc ttataatatt
     5101 agccaaacgg tgattccgga aatcgatcag cctttggaaa atgggtatac aaaagaagaa
     5161 cagaaaatga taaatgagac ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca
     5221 acctgcgtac gtgtgccaat cgaaaatgga catggagtct ctgtggcagt tcaactgcaa
     5281 aaagagttta cgacggaaga aattagagag tgtctcagcc agtttccagg gattcaatta
     5341 gaagatgatc tagcagcaca taaatatccg acgagcattc ttgctagagg aacagatatg
     5401 gtttatgttg ggaggatcag aaaagacact tctttagaga acggattgtt gttctatacg
     5461 actgccgata acatacgcaa aggagccgct gcgaatgccg tacagattgc tgcagcttta
     5521 atgcagaaaa tagcggaggt ggcaagatga gtaatacgct gaaagtagcg aaattcggcg
     5581 gaacatcgat gtctcatgaa gagcaattca aaaaagtccg tacaattgtt ctttcagatc
     5641 ccaatcgcca agtgatcgtt gtttctgctt taggcaaacg tacgtcagat gatgacaaag
     5701 taacagactt actttatttg atttatgctt accttcagca tcatgttgat tggacacccc
     5761 tttggaaaag gatttgtgat cgtttcattc aagtaagaga tgttcttgcc ctgcagttac
     5821 ctattgaaga tgaactagca cgcatacaga aacaactgga agacgaaaag ataactgaag
     5881 attatttagt aagtagaggt gaatatctga ctgctcaact gatggctgag tatcttggtt
     5941 accagtttat tgacgcaaaa gacctgattt tctttgacta tgatgggaaa atcgatcagg
     6001 aaaaaacagc acaggaaatc acaaaagcat ttaaaagata tccgaaaatg gttgttccag
     6061 ggttttacgg ggcgaaccct gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca
     6121 ttaccggcgc gatactggcg aagatgttgc aagcggaaag ttatgagaat tggacggatg
     6181 tttcaggaat catgatggct gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa
     6241 tctcttttag agaactgaga gagatggctt atatgggggc taacgttctt catgaagcag
     6301 ctgttttccc agttcaagag gctgatatcc ccatccaaat aaaaaatacc aatgatcctt
     6361 tcgctcaagg gacaaaaata tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa
     6421 tagctggacg caaggatttt ctttccatca ctttattcaa acgtcatatg tccgatgaaa
     6481 tcgggtttat ctggaaagca atgagcattt ttgctaaaca tgagatatca atcgaacata
     6541 ttccttcagg gatcgacaat atcggagtga tcgtctcagc agaagcgatt gccgataagt
     6601 tatttctcat cacgaaagaa ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg
     6661 aagacttagc tttgatctct gtcgttgggg gtccgcataa agaattgatt ggattatctg
     6721 ggaaagtgtt atcgatattg aacaaattag aaattcgcac atcgatctta tcacaaggag
     6781 cacaggagtt gaatctgatc atcggtgtac cgaataatca atatgaaaca gtagtgaagg
     6841 gtatctatga agggatggtg aatacggatg ctgaaagaat atcgaatggc actccatcaa
     6901 attcctgaaa tcgggtttca tgaattcaag acaaaaaaat atttatatga acaagtaaaa
     6961 aacagcgagg ccgttatcca tgaaatcggc gaaacaggac tactccttta ttttgataat
     7021 cagcaagaag aaacgattgc ttttcgaaca gatatcgatg cactgccagt caaagaagaa
     7081 acgaatctct cttttgcttc taagcatgaa ggttatatgc atgcatgtgg tcatgacgga
     7141 catatggcga tgcttttagg gttgactgac tttataacta aacacattga ggatcttatt
     7201 tataatgtag tactgatcta tcagccatca gaagaaattt ccggtggcgc acaaagcgtt
     7261 attcagtcag gattgttgga taaatatgaa gtacaagcaa tatttggctt tcatctatgg
     7321 cctggactag ctaagggaga agttttctct cgaccaggac ctttgatggc acaaagcagt
     7381 gaaacagaca ttatcattaa aggccagtct gcacatattg cttctagtga aaaaggaatc
     7441 gatagtctgg aggcagctgt acgatttttg aagaatgttt atgattttga ccggcaatta
     7501 ccggcagagt ggaatcatct aatgaaattt ggccaaatcc aaggtggaac gatccgaaat
     7561 gttttagctg gagaagttat tgtatccgga agtatccgtt cttacagttc agatatgcaa
     7621 aattatttga agaggggatt agaccattta gcagaacagt ttcactctac agctactatt
     7681 tcttttcgct ataatgacgg ctatccagct gtaatcaacc atagaggctt atatgcagca
     7741 ctgttagatt caaacaaact acatgaatta gatgctccag ttcttcaagc cgaagatttt
     7801 ggcatatata cgcaacaata tccatgtgta tttttctttt taggtgtcgg tgatacccct
     7861 gctttacatg atccgacgtt cgattttgat atggatgttt tagaaaatgg tgtagaatgg
     7921 tacaaaacga ttctttttac agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag
     7981 gagcgagtaa ataatgatga atttatccaa tcgatttagt cctaggcttt ctagaataga
     8041 agtatcaaaa atacgacaat tcgatcagca aatatcgtcg ataccagatg tgatcaaact
     8101 caccctggga gaaccggatt ttccaactcc tgaacatgtc aagcaagcag gaatagcagc
     8161 gattgaagaa gacttttctc actataccgg aatgagagga ttagaggaac tgcgggaagc
     8221 ggcttgtata tttcagcaac agcgttatgg attaacttat gatccgcaaa cggaagtttt
     8281 gacgacagtt ggtgcaacgg aagcgattgc ctctgcgttg ttatctgttt tggaagaagg
     8341 agacaaggtt cttattccag cgcctgctta ttcaggatat cagccattag tggaactagc
     8401 tggtgctgaa ttgatcccga tagatacttc tgacacaggg tttgtctgtc agccggaaca
     8461 atttgaacga gcatttgaac aatatggatc agctgtcaaa gcagtcattt taaactatcc
     8521 taacaatcct actgggacga cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt
     8581 aaaaaaatat ccggtatttg ttataagtga tgaagtatat gctgaattga cttatagtgg
     8641 aacgcatatg tccatagcta cttatcttcc agaacaaaca atcgtgataa gcgggttatc
     8701 caagtcacat gcgatgactg gttggcgagt gggctttatt tttgctcaaa aagcccttat
     8761 tgacgaacta attaaagtcc atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa
     8821 agctgcggct gaagcgctaa taaatggggt gaaagatagc gagaatatga aagagcaata
     8881 tcaaacacga agagactatc tggtccagca attggcgcct ttaggatttg aagtgaccca
     8941 gccaaacggt gcattttact tgttttgcaa actcccagct gccattcaaa ctgactcttg
     9001 gcagttttgt gtagaattag cggaacaggc aaaagttgcg tgtatcccag gaattgcatt
     9061 tggcccagaa ggagaaggtt atatccggat ttcttatgct agcagcatgg aaaaattaca
     9121 tgaggcctgc aaacggatca aagacttttt aacagaacag tgattcatag attttatcaa
     9181 aaaaacaatc gttaattttg cggtatattc tgcaaggaga cgattgtttt ttaaatttag
     9241 ttcaaatgaa cgcgtttctt ttgattcata actaatgtaa tgttaagttt aaattgttta
     9301 gaatgatttt aaaagaatat tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat
     9361 tgtaggtgca tctcatggag ggcatcaatc gatcttggaa ctattgacac gatacggtga
     9421 aaatgtagat atcacgctat ttgaagcagg agactatatt tccttcatgt catgtggtat
     9481 ggagctgtat ttagaagaca aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga
     9541 aaactttccg caatccaatg ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc
     9601 cgataaaaaa acagtaacgg ttactagcgc atcagataac gaaacaaaag aatatcctta
     9661 tgacaaattg attttgagtt ctggtgtcaa accaaacaat ctgccagtac caggagcaga
     9721 tctagaaaat gtctacttga tgcgtggata caattgggca accaaaatca aagaaaaatt
     9781 aaatgatccg gctattaaaa aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc
     9841 agcagaagca agccgaaaag ctgggaaaga agtcgtattg cttgatgtga tcgatagacc
     9901 tttaggcact tatttagatg ctgagatgac agatatttta gaacaacacc taaatgaaaa
     9961 cggtattgaa gttttgacaa atgcaaaaat cgaagctttt actggtaacg gaaaagttga
    10021 ggcaatcaaa acaagtgaaa aagaaatccc agcagatcta gtcattcaag ctgccggtgt
    10081 gaaggcaaat acagaatggt taaaagggat cgtcgactta gatgaacgtg gctggatcca
    10141 aacaaatgaa tacttgcaaa cgaatcttcc agatgtgtat gcagtaggag atgctacact
    10201 tgcttattct atcccagcac gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag
    10261 agaagcacgt tatgtagtaa aacatctttt tgaagaagta ccaaacaaac cgttcggcgg
    10321 agtcgtaggt tcctctgcat taagtgtctt tgactatcat tttgcagcaa gcggattgaa
    10381 tagctttaca gcagcaaaag caggagtaga tgtacgagtg tcttattatg aagatacgtt
    10441 acgaccaaaa tatgtaccag aagaacaagg aaatccaaaa gtgtccatcc aattattctc
    10501 ggatccaatg acccatcaat tgctaggagg agcagtatta tctacctatg atgttacagg
    10561 tcaaggaaat gtattggcac tagcgatcca aaacaaactg actttagaag aactagcaga
    10621 agctgacttc ttcttccagc caggctttga ccgacaatgg agcatgttga atctagccgc
    10681 tcaacaagcg ttaggagaag aaccatttgt agaataagaa aacgaaatat ttgaaaagag
    10741 gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaa
//
LOCUS       DASJON010000098        10759 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJON010000098 DASJON010000000
VERSION     DASJON010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.098"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(58..1041)
                     /gene="istA"
                     /locus_tag="U3989_001804"
                     /pseudo
     CDS             complement(58..1041)
                     /gene="istA"
                     /locus_tag="U3989_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1436..2278)
                     /locus_tag="U3989_001805"
     CDS             complement(1436..2278)
                     /locus_tag="U3989_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1715255.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2603..3019
                     /locus_tag="U3989_001806"
     CDS             2603..3019
                     /locus_tag="U3989_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1715256.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3016..3486
                     /locus_tag="U3989_001807"
     CDS             3016..3486
                     /locus_tag="U3989_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715257.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3500..4273
                     /locus_tag="U3989_001808"
     CDS             3500..4273
                     /locus_tag="U3989_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715258.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4287..5099
                     /locus_tag="U3989_001809"
     CDS             4287..5099
                     /locus_tag="U3989_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1715259.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5153..6118
                     /locus_tag="U3989_001810"
     CDS             5153..6118
                     /locus_tag="U3989_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1715260.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6254..8977
                     /locus_tag="U3989_001811"
     CDS             6254..8977
                     /locus_tag="U3989_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1715261.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8990..9622
                     /locus_tag="U3989_001812"
     CDS             8990..9622
                     /locus_tag="U3989_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1715262.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9827..10054
                     /locus_tag="U3989_001813"
     CDS             9827..10054
                     /locus_tag="U3989_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEN1715263.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10123..10389
                     /locus_tag="U3989_001814"
     CDS             10123..10389
                     /locus_tag="U3989_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1715264.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10379..10648
                     /locus_tag="U3989_001815"
     CDS             10379..10648
                     /locus_tag="U3989_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1715265.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3565 a         1716 c         2102 g         3376 t
ORIGIN      
        1 ataaatagtt tttctatcaa gtatattgaa ctacgataaa tttgttattt tgttttgttt
       61 aatttcgttg tacttattgg ctttgacttc gatttcttta aatactgggt aatcggtgtc
      121 cggcaaagct agcaaatctg ttttttctgt gttccataga tcattgataa tgacatgttt
      181 ttcgtaatgg attcgctctc gatcgtgtga cagctccacc gctaatgcct cgttgaaact
      241 ggcgaaatcc ttcataatcg gtgtagatga aaagaagttg taccgtacat agccgacttt
      301 gttttccata ctacctttct cgtttccgct acgtggattg cacacctgtg tctcaaaacc
      361 atagtgcata gaaaatcgga ggaaaccgtc agtcagcacc gcttgttcag tctttgtttt
      421 tgtctaagta acggccgttg acatattatc aatccgtatt ttccgaggaa cgccacctga
      481 ttggctgaag agttctttca tgccttccaa caaacattct tgattctcgg cggggagtac
      541 aacggcgaat cctgcattac taaaagggaa agtcatcact aataccttca catctttaaa
      601 cgctccatcg tgacaaactt ccatcgtacc aaaatcaagt tgcgcttctg ctggcgggtg
      661 ttcaagacgc tcaaagcctt ggtcttccag tgtttccaag tagtgtgtcg ctttccaatc
      721 ttgaatgaat acacagactg ttcggtagga tcctggaaag ccatcggctt ttaaagcagt
      781 agtcattgct tcagcagtcc tacgcttttt atttggcagc ttagcatctt ctgaaagcca
      841 tagggaaacg atgcctcccc acttttctgt atacatcata ccagtctttt tgcgtgcttt
      901 aggttgcggc agcaaatcat catctgcata ttttttagcc gtgcgccaat ttacatctag
      961 ctcctttgca atatgattaa tggatttatc atgattattt cgtaaatttc tgatagtatt
     1021 aatatcggtc attgtgagca tacctttctt acctccagtt acagtgtcga gactataact
     1081 ttagaggagt ttcaggttgc ccgcaatggc tttttatctt aacttaatcg atttgcggag
     1141 ttgtgtagtt tttcgttgca cttgtatgta cttttattct gccataaaca gcttaatgaa
     1201 tgtccacttg aaaaagtgaa taaacgaaat agatgactcg atttaataag gaagtaaacc
     1261 cacacagaaa tataggtgat tttccttgaa tgaccaagat attgaaagtt agtgaaaaaa
     1321 cacaaaaagg tagccctatt cacagaaggt ctgccttttg tgttatcgcc agcaaacatt
     1381 agagggggta tttcatatag cttttgtcct ctcgacagtc ctatcagttt ctcttttaac
     1441 ttatctctaa gtgctgtcca gttttaatag cttctagctt gtttcctaac tgtgttttta
     1501 atatgttgaa ggcttttgaa cctgtgcagt ggcaagtaag gattttttct actcgtgagt
     1561 cattcaattt atttccaatt ttcattacct cttgctcagg tagacctaaa ttatttatat
     1621 aaggtatgcc aatcatatgg aagccaccaa ttaggtattt tatgggttta gtcggaaatg
     1681 tttcaagagc aatttccatc atatcgataa tgccatgatg actacagcca gtaaagacaa
     1741 tcagaccgtc ttcttgctca attacgacca ataattcatc tgaaaaatca tcataaacaa
     1801 atttatcatc catctttttg tataaattct tggtggtcga gtttgctttg cctaggggga
     1861 tgatatggaa gctcggagca agttctgttt tcgtttttat tgtatgtaaa ttctcatgca
     1921 ttttaaggat ttcattttta ggaataccaa catacagaaa cgtggttaaa atctttaaat
     1981 aatggggtaa agttacacct tcagctgtat aaataggggc gtttcctgat tttaaatatc
     2041 ttaacccgcc ggtgtggtca gaatgaccgt gtgaaataat cactgcctcc agatcattca
     2101 aagtttcccc cattttagtc gcattataga ctaatttttt attagcgcca gcatcgaaga
     2161 gatatttctt ccctgagtag ttaattaaga gagataaccc atgtccactt ttaaagttca
     2221 aatttattct tgagttttca aataaaacct tgatttccac tataacatct cctttcacat
     2281 gaacgtgtat tcatatattg tagcacatta agttctattt tttgttgtaa ttaccgaaaa
     2341 aaatattaat ggacgctttt tacgctttat ttcgtatgtg gaagaaaatt tcttaggatg
     2401 tggcggggga ttggggcaaa gctaaattcc aattacagtg atcatttaat tagacacctt
     2461 taaataccca cactttcatc aacactataa tttagggaaa gaaaagtgtt ggcatgatat
     2521 agaaaaacct ttaattaaca gcgtttccta aaattggcac gcatcttgct tatagaaaag
     2581 cgtaaggata ggaggaatga aaatggcaac tataattctt gccagccatg gggaatttgc
     2641 tcaaggatta aagcaaacag cgacaatgat tattggagat agtgtaccaa ttcatgcctt
     2701 gtcagcattt agagatgaag atgaatctat tttggtacaa ataaaaaaac ttttagccac
     2761 aatagatata gaagaaacct acattttaac tgatattcta ggtggtagtg tgaataatga
     2821 tatgctcact attattaaag agtatgaggg attgcaatta attactggaa tgaacttacc
     2881 tttagttatt tctattgcaa cctataatgg aaaaattggg gaaagtgagc tagaaaagct
     2941 tattaaagag agtcaacaat cagtgattga ttgtagaaaa ttattagtag aacaacttac
     3001 tggaggagat gacctatgat taagttagta cgtattgacc atcgtttgtt gcacggacag
     3061 gtagtatttt cttggagtaa atcattaaat atcaatcgta ttattattgc caataaagag
     3121 gcagcgaatg atgaattcaa aaaaatgtct ttgaatttat ctaaaccgac tggcattaaa
     3181 ttaacaattt tttcagttga acagttacta gaaaaaatgc ctaaaatcga aacacttaac
     3241 gaaaatatca tgcttatttt tggtaataca aaagaaattc gtcaattttg tgaagcctat
     3301 cctaaagtac aagaaatcaa ctatggaggg ataatcaaaa aagaaggttc caagcaattt
     3361 agtaatgcca ttttcttgaa tgaatctgag caagaagatg ccaaaaaatt aaaagctctt
     3421 ggcattaaac aatttattca acaggtacca acttctaaaa aagaagattt gaatacaatg
     3481 ttataaagga ggaatgaaaa tgctcgttca gtcaattggt ttagggttaa tcggtgtttt
     3541 ttgtatttta gactcaaggc ttttaggaag aatgaatttt gaacgaccgt tgattgtcag
     3601 tacgcttgtt ggtttggtac ttggagacat ggaaaaagga ttaattgttg gagcctcttt
     3661 ggaattgatg tccttaggat tagtaaatat cggagctgct tcaccacctg atatgaatat
     3721 ggcatcaatt attgctacag cctttgccat tttatcaaat gcgaatgcag aagcagcatt
     3781 aacgattgcc attccaattt cagtgttagg tcaaatacta ggtatcttaa tgcggacgat
     3841 tttgtcaaat ttgactcacg ttgcggatcg ctttatcgaa caaggaaaat ttagccaagc
     3901 aaggagaatt catatacttt atggctcggg attatatgct ctcatgtatt ttattcctat
     3961 ttttctagca atttattttg gaacgaatct tgtttctcag attgttgatg cgattcctga
     4021 gtggctaaca aatggcttaa cggttgcaag taaaatttta ccagcatatg ggtttgcgtt
     4081 gttattacaa acgatgttaa cgaaaaaaat ggtgccattt ttattacttg gatttttaat
     4141 tactgcttat tctggattgg gaatcattgg tattgcagcc ttcgcatgta ttgttgcgtt
     4201 tgtcatggtt caagttcaag gtaaaaactc aaatggtaac caaaatgagc cagatccatt
     4261 agatgactta taaggaggta gattaagtga aagagaaaga tcaaaatgca tggaaatatc
     4321 ggcagttctt atggcgttca tgggcaattc aagcctcatg gaactatgaa cgacaaatga
     4381 acatgggttt tatgtacgga attgcaccaa ttctagataa catttacaag aatccagaag
     4441 atgaagcaat gaagaaagaa gcttacgagc ggcatatgat gtactacaac tgtacccctc
     4501 aaacaagtgc atttgtgtta gggttaactg cttctatgga ggagcaatac tacgaagacc
     4561 aagaaaactt taatccggaa tcaatcagtg cagtgaagac ttctctgatg ggaccgctat
     4621 ctggtgttgg tgattctttc tttcaaggaa cgattcgggt attagccttt ggactaggaa
     4681 ttaatttagc ccaacaaggt agtattttag gtccaattct agcaatgtta atttcctttg
     4741 taccttcctt tttagttact tactatggtg ggaaaattgg ctataacatg gggaataaat
     4801 acttgtctaa actgtatcaa gaaggtttga tggataaagt aatgtatgtt tgctccattg
     4861 tggggttgat ggtcattggt tcgatgattg ccagcatgat cgggattaca acaccaattc
     4921 aaatgggtaa agcttttgtc ttacaagatg tattagatag tattatgcca caatttttac
     4981 cattaggatt aaccttcttc atgtactggt tattgcagaa aaaggtcaaa acaggatgga
     5041 tgttggcaat ctgtttagtc ggaggaatta tttttagttt gttaggtatc ttacaataag
     5101 ggaataacca ataaatttgg ttgaataata taaataagga ggaattcaat tgatgaattc
     5161 aaaagaaatt gttaaagaaa tcgtagcgaa aaaagaggta aaatcaatcg tatttgttgg
     5221 ctgtggcgct tcaaaagcgg acctttatcc tgcaaagtat tttctagacc aaaatgcgaa
     5281 gcagttacgg attagtcatt acacagccag cgaatttaac tatgcaacac cagcatctgt
     5341 agatgagtca actgtcgtta tttcagcttc tttaggaggt acgactccag aaacagttga
     5401 agctaacgca gtagctaaag aaaaaggtgc tacagttatt agtctaacac gaagcattga
     5461 ctcaccgtta acaaaagatg cagattatgt aatttatcat cgatttgcag agaactatgc
     5521 tgctaaatta gaaaaaacag gctactgttt agaattggca gtagaacttt tacatcaagt
     5581 agaaggttat gaccattatg aagcaatgca agaaggattt gctactatct atgatcttgc
     5641 ccaaaaaagt gcagaatcag ctcgtaaaga tgcgaagaaa tttgctgaac gctttaaaga
     5701 tgaaaaagtt atttatgtaa tgagtagtgg tgctacacat gaagtagcat actcaacatc
     5761 aatttgcttg atgatggaaa tgcaatggat taattctggt accttccatt caggagaatt
     5821 tttccacgga ccattcgaaa ttgtggataa agacgtaccg tttatattat taatgaatga
     5881 tggaaaaact agaaatatag atgctcgagc attaactttc cttgaacgct ttgatacgaa
     5941 aacagaagtt gtagatgcct tggattatgg cttgtcttca catattgcta aagaagtctt
     6001 agactacttt aacccattcg tgattactgc agtatttaga gtttatgcag aagaattgtc
     6061 agaagcaaga gaacatccat taactaagag acgttatatg tggaaactaa cttattaaaa
     6121 aatagatcaa actaaataag gtggcttgag aaattaagcc accttgtttt cttcaatgtg
     6181 attttataca tatggtaaaa taactagtac atatggtaaa ataactagta catattgtga
     6241 aagcaggtgg cagatgcagc gattaaatga ctatctttat caactaacat tgcttttgaa
     6301 atctacgcct tcagataatt attcagctcg cagtatttcg caagctctac agttgaatag
     6361 aagtacaatc agtagctact taaatgaagg tgttcgtgaa gggcttttca taaaagtaaa
     6421 aagttacccg gttttatttt tacatagaac agctcttgaa gaactacata tcacactgaa
     6481 taactccgaa atagaatcaa ttgaaagctt gttaacaatt tctcaaaaac ccgcattaga
     6541 tcaagtaatt ggctctaaag gaagtttgaa agaagcaatt gatcaaatta aaacggcagt
     6601 cttatatcct ggaaaagggt tgcctttatt attgatcggc gctagtggat cagggaaaac
     6661 ctttctagcg aataaaatat atgaatatgc agtagaagaa aaggtcattc acacatcggc
     6721 acctttcata gattataatt gtgcacaata tgtgagcaat ccagagttac tatcctctgc
     6781 tttatttggc tacacgaaag gagcatttac cggagcaagc caggagcata ctggtttgct
     6841 agaaaaagcc gatggaggag tattgttttt agatgaagtg catcgcttat ctgaagaagg
     6901 gcaagagaaa ttatttacat ttatggatac gggtgaattc tctcctatgg gggataatag
     6961 tattcggaaa aaagccgatg ttcgattagt ctttgcaaca acagaaaata tatatacgac
     7021 tttcttacca acttttctta ggagattgcc agttattgtt aatctaccta gatttcaaca
     7081 acgtccgtcg tttgaacgtc tgtcattgat tgatgaattt tttgtttctg agagccaaat
     7141 tttagctaag gaactatctg tttctgatgc attgattcat ttcttaatga atagtgattt
     7201 tgaaggtaac gtggggaata ttaaaaatat tatcaagtat gcttgcggaa acgcatatat
     7261 ccaccagaag agtcagcaaa caattcaagt taaattattg gatatgcctt tagagtacag
     7321 ttataaattc aaggagcaac taaataaacc cagaaagaaa agagcagatc gaatctacta
     7381 tccagaagaa acacgacaag tgcaactaga aagaaacaat cagctttttg ttcaattttt
     7441 tgaccaatta cttactggat tctctgaagt gattgaaaat aacaaggata ttaaactatt
     7501 tttagaagaa atggttacca aggtaacaca agtaatggat acgcttattt ttcaagagga
     7561 ttacgaaaaa gagcagtctt tatacgccat tctaaactat catattcgtc aaagtattga
     7621 ttttatgcga gaaagctacg ggtttgaaca agatggtaac cgggtagtcg ctttggccaa
     7681 ctatctatat gtcaaggata atcaagtgat ttttgaaaat agtcttcaag aaaagaaaac
     7741 caaagatcag cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa
     7801 aaaaatctta agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt
     7861 ctttattacg ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag
     7921 tattgtgcta gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat
     7981 gctgaataag aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat
     8041 tgaagcaaaa ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt
     8101 ggtagatatg ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc
     8161 actgttaatc attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt
     8221 aaacaataaa agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa
     8281 gcagttacta cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg
     8341 gattggtagt gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag
     8401 agaattagta attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac
     8461 tccttttcaa ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca
     8521 gattccatat attggattag atcgcctgat taatggggga agcattggcg aatttgttca
     8581 ttacttggaa aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa
     8641 tttttcaatt agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc
     8701 ttttgtacaa aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa
     8761 tcagctgttt ttgttgtacc ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga
     8821 aagtgtggat gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga
     8881 taaaattcat agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt
     8941 agagttgcga ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg
     9001 agtaattttt gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag
     9061 aatgaacttc tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt
     9121 ggggaaaaca gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga
     9181 ggaactttat ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc
     9241 tttacgaaaa gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc
     9301 actggcatct tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac
     9361 tgaatatttt tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga
     9421 aatatattta aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc
     9481 aacgattggt attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc
     9541 aatgcaaatc gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca
     9601 aattgtttta gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct
     9661 attttgacta accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac
     9721 cttttacgaa aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt
     9781 tcgcaatccc taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa
     9841 ccaaaatacg aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac
     9901 tgatactact gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat
     9961 ccctgaaaat tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag
    10021 attttgcgat accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt
    10081 tgtactaacg ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg
    10141 aacggaggta gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa
    10201 gaactggtat tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat
    10261 gctgtactgg ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa
    10321 gtttccaaag agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat
    10381 gaattataaa gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa
    10441 ccaagctaaa ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg
    10501 tcagcacgga aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa
    10561 ctatcgaata ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg
    10621 acatagaagt acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt
    10681 caatgtataa tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg
    10741 gttggtttct gctttttta
//
LOCUS       DASJON010000099        10586 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJON010000099 DASJON010000000
VERSION     DASJON010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.099"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..722)
                     /locus_tag="U3989_001816"
     CDS             complement(24..722)
                     /locus_tag="U3989_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN1715266.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="U3989_001817"
     CDS             complement(715..2067)
                     /locus_tag="U3989_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN1715267.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="U3989_001818"
     CDS             complement(2290..3690)
                     /locus_tag="U3989_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN1715268.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="U3989_001819"
     CDS             3851..4588
                     /locus_tag="U3989_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN1715269.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="U3989_001820"
     CDS             4713..5192
                     /locus_tag="U3989_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN1715270.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="U3989_001821"
     CDS             5189..5956
                     /locus_tag="U3989_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715271.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="U3989_001822"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="U3989_001822"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1715272.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="U3989_001823"
     CDS             complement(6859..7461)
                     /locus_tag="U3989_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1715273.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="U3989_001824"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="U3989_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="U3989_001825"
     CDS             8142..8726
                     /locus_tag="U3989_001825"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN1715274.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="U3989_001826"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="U3989_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN1715275.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..>10586
                     /gene="prmC"
                     /locus_tag="U3989_001827"
     CDS             9795..>10586
                     /gene="prmC"
                     /locus_tag="U3989_001827"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN1715276.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAI"
BASE COUNT         3632 a         1907 c         2085 g         2962 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgata
//
LOCUS       DASJON010000100        10347 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJON010000100 DASJON010000000
VERSION     DASJON010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.100"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(64..306)
                     /gene="acpP"
                     /locus_tag="U3989_001828"
     CDS             complement(64..306)
                     /gene="acpP"
                     /locus_tag="U3989_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1715277.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(428..1429)
                     /gene="plsX"
                     /locus_tag="U3989_001829"
     CDS             complement(428..1429)
                     /gene="plsX"
                     /locus_tag="U3989_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN1715278.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1478..3514)
                     /gene="recG"
                     /locus_tag="U3989_001830"
     CDS             complement(1478..3514)
                     /gene="recG"
                     /locus_tag="U3989_001830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN1715279.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3631..5313)
                     /locus_tag="U3989_001831"
     CDS             complement(3631..5313)
                     /locus_tag="U3989_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN1715280.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5365..5727)
                     /locus_tag="U3989_001832"
     CDS             complement(5365..5727)
                     /locus_tag="U3989_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1715281.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5997..6185
                     /locus_tag="U3989_001833"
     CDS             5997..6185
                     /locus_tag="U3989_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN1715282.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6423..9092
                     /locus_tag="U3989_001834"
     CDS             6423..9092
                     /locus_tag="U3989_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN1715283.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9547..10293)
                     /gene="truA"
                     /locus_tag="U3989_001835"
     CDS             complement(9547..10293)
                     /gene="truA"
                     /locus_tag="U3989_001835"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1715284.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2819 a         2231 c         1860 g         3437 t
ORIGIN      
        1 attaaaaaaa gaaggataaa aagctgcatg cagcttttta tccttctttg cttttatttg
       61 aaattattta agatggctgc tgatataatc tactgcgcca ccgactgttt caatttgttc
      121 agcgtcttca tctgaaattt ccgtaccgaa ttcatcttca agctctaaaa cgaattccat
      181 aatactaatt gaatcagctt ttaaatcatc tttgatgttc aattcatccg tcactttatc
      241 ggtatctatc tcaaaatgat ttgaaataat tttcgctact ttgttaaata cttcttcgcg
      301 agtcaacttt ttcacctcca agaatcatac acttgtcaat tacctacacc atggctaatt
      361 ttactggcat ttttcaggaa tgtccacctt tttttcaatg gaacggatcg cttcagatat
      421 ccaaatacta ttcttcgctt ttttcaaatt gttcgactaa ttgtccaact acatctgtct
      481 caagcattgt gtggatttgg cggatcgtat agcgaacagc atcggggcct gttgctccat
      541 gagttttaat gactggtgct ttcaagccaa acaaaacggc accgccatgt ttggaataat
      601 ccatttctgc tttcaaagaa cgtaagccgt cttttaaaag catagcgccc atttttcctt
      661 tgatcccttc atttagaata gcagatttta atagattcat catattcatt gctgttcctt
      721 caatagattt caaaacagcg ttgcctgtaa atccatctgt gaccactaca tcagccacac
      781 cattgagtag ctcacgagct tctacgtttc caataaaatt caagcttgtt tcattctgaa
      841 ggagttcata ggcttttttc gtcacttcac taccttttgt tgcttctgtt ccgttgttta
      901 agagtgcgac acgaggacgt ccgatacctc ggactttacg tgcataaaaa gaacctaaaa
      961 taccgtattg caacaaatgt tctggtttgt tttctgcgtt agcgccaaga tccagcatat
     1021 cgaaaccggc accttctttc cctactactg gcaaggtaga catcaagcca gggcgttcga
     1081 tatttttgat acgtcctacg atgaacaacc ctgctgctag tagcgcgcct gtattccctg
     1141 ctgaaaatac agcatctgct tcaccgtttt tcactgcttg tgcagcaagg accattgatg
     1201 cagtcttttt tcgtcgaatt gcttttactg gctcatcatc actattgatt ttttcgtctg
     1261 tatggataat cgtgatgttt ttttcatttg ttaagtattt cttgatttct gcttcttttc
     1321 cgtaaagtaa gaattctact tctggaaaat cttgctgcgc taaagccacg ccttctacaa
     1381 ttgcttgagg tgcgtgatct ccgcccattg catctacggc aattttcatt gtttccactc
     1441 ctttttccgt catcagtata aagtatagca cagtctgcta atcgaaataa cgattatcct
     1501 gattttctat ttggaggctt tgcgctaaag gctgatactc aggaagcatc tgccaatcct
     1561 ttatttgcca aagggctgca gcctcttctc tggctacctc caaaacattt ccatccgtca
     1621 ccaagtcagc aatcgcaaat tgaggcagac cagattgacg gaagccaaat acttcaccag
     1681 gaccacgtaa ttccagatct ttttcgctta gtacaaaacc attggtggtc tctgtcatga
     1741 ttttcatccg ttccacacct agttcatttt taggattagc aactaagata cagtaggaag
     1801 catcacttcc gcgtccaaca cgtccacgaa gttggtgcag ctgtgccaat ccaaatcgat
     1861 ccgcatccat gatcagcatg acggtcgcgt tcggcacgtt tacccctact tcaattacag
     1921 tagtcgaaac taatatctgc atctggttgt ctttgaaggc ttccatgatt acttcttttt
     1981 cctgattctt cattttacca tgtaacaagc caacttcata ttttggtgca aatagttcac
     2041 gaagtttttc atagatttcc actgcatttt ttacatctaa tgcttctgat tcttcaatca
     2101 gcggacagat gacatacatt tgatgtcctc ttgccagttc ttttttcgtc caatccagta
     2161 cactgtccaa ttgaggtgtt cttacccagc gtgtttcgat cgggatacgt ccagctggaa
     2221 gttcatcaat gatagataca tccatctcac catatgctgt aatagctaat gttctaggaa
     2281 tcggagtcgc tgtcataaat aagacatctg gtcgccagcc tttttcacga agtactttcc
     2341 gctggttcac gccaaaacgg tgctgctcat ccgtaataac tagacctaaa ttctgaaatt
     2401 cgactccatc ttggatcaat gcatgtgtgc cgatcacgac gttaatttcc ccttgctcta
     2461 gtttttcaag gagctcacgt cgttcttttg ctttcgttga ccccgtaatc aatgcgatgg
     2521 ttacttcatt gggatcaaac agctgttgca agctttccat atgttgttgt gccaagattt
     2581 ctgtaggcac catcaacgca ccttggaaac ctgctgtaac ggtagcgtat aacgcaatcg
     2641 cggcaaccac tgtttttccg ctacctacat ctccctgcaa aagtcgctgc atatgctgcg
     2701 gactgcgaag atcccgacag atttcattcg ttacacgttt ttgtgcattt gtcagttcaa
     2761 aaggtagctt ttgagtaaat tgtttcaatt tttggacatc ataaaggatc tccaaaccat
     2821 tcgtttctgc tttttctgct tttttcaatc cttgaagacg catttgaaat aaaaagaact
     2881 cttcaaaaac gacacgtcgt ttggcttgat ggttttcttc gtggtctttt ggaaaatgca
     2941 tacttcttac agcagaagct cggtcaagca gtcgatattt ctccacaagg ttgtttggca
     3001 gattttcttc aatcaatgtg ccgtattctt gaaacccttt tttgattaga tcaactaatg
     3061 tcgtttgacg aattgatttg ttgacgtgat atatcggcga aaagtcatcc attgtttgag
     3121 aacctaaaat cttcatcccg ttcaaggctt tacgctttgc atcccattta ccgtatacgg
     3181 cgatttcctc cgataaaatg actttttctt ttagataagg ttgattaaaa aaagaaacgt
     3241 tgaacacatc atgttcttgc atcatacgaa attgcaatcg gcttttttta taaccaaatc
     3301 gattcatcac aggaggcgag actacgattc ctttcaaagt aaccttttct tgatcctgga
     3361 tctcgtttag atttctttcc tgaatatctt cgtagcgaaa aggataataa gtcagtaagt
     3421 cttcaatcgt ttggatgccc aagctggcaa gactagctgc tcttttttct ccgactcctg
     3481 ccagcatagt gattggatca tctaaagaac gcataaattt ttcctccctc ctttaacaaa
     3541 aaaaggactt ggtcagcgat catacctgat cactaaccga aggcctttta tgaatgaata
     3601 ctgtgttaga aagatattct gacacagaaa ttattcagca gaaaatagat aaggatagac
     3661 aggctgatct ccttggtgga tctcgatttc caaatcagga tgtttttctg ttagtttctc
     3721 agcaaattca tttgcttgtt caactgttcc ttcatcacct gccaagatcg ttacgatctc
     3781 tgtgtcctca ttgatcattt gttctagtgt acttaacgct gttgctgtga tatctgggtt
     3841 agagatcagg atttttccat cgatcatgcc caaaaagtct ccttctttga tctctacacc
     3901 gtcaattgag gtatctctga tcgcgtgagt gacttgaccg ctcacaacgc tagacaacat
     3961 ttctgtcatc gtttctttgt tttcttctaa agaagcttga tcattgaatg aaagcatagc
     4021 tgtcaatcct tgggaaatcg ttcttgaagg aacaactgct actgggatat ctgctacttc
     4081 tgctgcttga tcagctgcca tgaagatgtt cttattgttt ggcaagacaa tcactttatc
     4141 tgcgtttact tcttgaatcg ctttaacaat gtcttccgta cttggattca ttgtttgtcc
     4201 accactgata acataattag cgcctaaact ttcaaacagc tcttggacgc ctttaccagc
     4261 agcgatcgct acgataccat acgggatacg tggtatttct tttttcgcaa actcgttcac
     4321 ttcttcatcg tgttccaaaa tcgtctcatg ctgcaagcgc atgttgtcga ctttgatttt
     4381 gactaatgaa ccgaattttt gtccataatt caagacttct cctgggtttt ctgtatgcac
     4441 gtggactttg atgatctcgt catcattgac cacaagtaaa gaatctccta taccatctaa
     4501 atagttacgg aattcttcgt aatcgaatgt actatcgact gtaggtcctt ctccgatttt
     4561 taccatgatt tctgtacagt aaccaaaatg gatatcttct gtcgccattt gaccttggac
     4621 actgcgatga tgttctgcat tgaccatatc atccatttct gctggagaag gttcatatgt
     4681 ttcatccgct tggaattctc cattcaatgc acttaaaaag ccctcataaa caaacagcag
     4741 tccttgccct ccactgtcta cgacaccgac ttcttttagg actggaagca agtctggagt
     4801 tttagctaaa gcacgctttg ctccttttac aacagctgtc attacttcaa cgacatcatc
     4861 agtacttttt gctttctttt ctccagcttt cgctgcttct ctagctactg tcaaaatcgt
     4921 tccttctact ggtttcatta ctgctttata cgcagtttca actccatgag tgaaggctgc
     4981 agcaagatca gaagcattta atgagacaac atctggaatt tgcttagaaa atccacgaaa
     5041 caattgagac aaaataacgc cggaatttcc gcgcgcaccc atcagcaatc cttttgaaag
     5101 cattgctgct aattctccta ctttttctga ggttgaatct actactgcct tagctccact
     5161 agtcatagat agattcatat ttgttcccgt atcgccatct ggcacaggga atacatttaa
     5221 tgaattgacg tattctgcat ttacttgcag acgattcgca cctgcctgga ccatttcctg
     5281 gaactggctt gcacttattt ctgttacact cacctagata atcctccttc agatcgactg
     5341 ctgagttacc agccagtctc ctgcctagtc tggcagtaca cgaacacctt gtacaaagac
     5401 attaactgaa ttggcagtta cgcctaataa tgcctctaga ttgtatttca ctttttcttg
     5461 gacgttgcgg gaaacttcag agattttcgt accatagctg ataatcgtat aaacatctac
     5521 agcaatacca ttatcttctt ggcggacaac tactcctctt gagtaatttt ctttacgcaa
     5581 aatctcagta atattatctt taatttgatt tttgctggcc attcctacaa caccgaacac
     5641 atctgtcgcg gcgccaccaa caactgttgc aattacatca ttggtaattt caatggttcc
     5701 agctgacgtt tgaattttta cagccatgaa tgagcctcct taacgggtaa aaaactccca
     5761 tttttcttct atcatatttt atcatagcgc aacttgaaat aaaaggcagt tacccaaagt
     5821 actcaaaaac cttgcaaaat ttcaggtata accttgttta atttacaatt attcttctca
     5881 attgtcaatt gtatctatga aaatgtactt gcaaattccc attctatatg atatgttagt
     5941 ctggtatgag ctgaattatt ctattgctcc aaatcaaggc aaaggaggaa attaccatgg
     6001 caaaagtttg ttactttaca ggtcgcaaga caaaaagtgg aaacaaccgt tcacacgcta
     6061 tgaactcaac aaaacgtact gttaaaccta acttacaaaa agttcgcgtt atggtcgacg
     6121 gaaaacctaa aaaagtttgg gtgtcaactc gtgctttgaa atcaggaaaa gttgaacgcg
     6181 tttaattgaa tgatacttgg accgttcaga tagtgaacgg ttttttattt aataaaaatt
     6241 tttaagggtg ggacttttga ttcctaccct ttttttgcgt ttatttttgt tatacgaaca
     6301 aattatgaag ttatttattc taaattttat ttttttgtga aaactagact ctttttaagc
     6361 agagaaaaac aaggatatga taaactgaat acaacttcat agaaatttgg tggaaaataa
     6421 atatgaaaaa aaatttaaag tcattcttta gagaaaacgg acgctacgca gcagctagtt
     6481 tcctgattcc gtttttgatt ttagcacttg tttatctaag tatcggaatc tatcctggaa
     6541 gcagcagaag tattttggca agtgatgcct tttcacagtt ttctaatttt catgccagct
     6601 tccgaaatgt tcttcttgga aagcaaagta ttttttatac atggaatgct tctctaggat
     6661 tgaattattt atctttaatt tcttactatc taggcgggat cttcacgcca ctggtgttct
     6721 tttttccaaa ccagatgatg ccagatgcac tgtatgttct gactttgctt aaggtcggct
     6781 gtgccggatt ggctttttgg tttcttgcac gtacgttcaa gattcctcgc tgggggcatg
     6841 ttgcacttag cgtcagttac gcattgatct catttatcac tgcacattcg gagatcatca
     6901 tgtggctgga tgcatttttt tatcttccat tagtgatttt ggggattcat cgtgttatgg
     6961 atcttaaacg gccgaaactt ttatttatca gttatttact cttgttttta tccagttttt
     7021 atatgggttt tatgattggc gtattttctt tcttatattt tatcgcccgt ctattgacaa
     7081 actggtcaga atataaaaaa acgattgttc cttatggtat cacttcttta ctggcaggcg
     7141 gcgcttcaat gatcattgta ttgccagcag tgctggatct tcggtcaaat ggcgaggcac
     7201 tgagtcagat cactacgttg aaaacagaag cgacttctta tctggatcta atcatgaaaa
     7261 atatgattgg tgtttatgac acgacaaaat acggatcgat tccgttcgtt tacgcaggtc
     7321 tgctaccatt agtcctctgt ctgttttatt ttgccagtcg gaaagtaccg ttgaaaaata
     7381 aatttttgta cggcagtctt ttcgccatac ttatcgcaag tttttatctt gttccattga
     7441 atttgttctg gcatggtatg catgcaccga atatgttttt attccgttat gcctatctgt
     7501 tctctttctt agtgctcctg cttgcaggaa aaggatggga aaagatagaa caagacgatc
     7561 gtgggctgtt gttaggaatc attttggtcg tagctgctct ctttgcggtg gcatggggca
     7621 tcaaacctga aggaagctac acctatgtga cgatgacttc ctttgtattg acagtggttt
     7681 tcttagggat ttatgctttt gctgtgggat tttatcagca taaaaaactt tcattcaatc
     7741 atctgagtat attgttatta ttgatcatgt ctgctgaagc actggtcaat acgaacagta
     7801 tgatccatgg cattcttgat gattggaatt atgcttcccg cagtttatat actgatcctt
     7861 atcctgactt gaaagctctt gtaaatgaaa caaaaaaaga ttccgactcg ttttaccgct
     7921 tggaaaatct ggatccagta tctgcaaacg attcgatcaa ctatggttac agtggtatca
     7981 gtttgttttc ttctataaga aatcgtaatt cttcctctta cctggataaa ttaggtttcc
     8041 gttcaagagg gacaaatttg aatatccgtt ataacaataa tactttgctg atggatgggt
     8101 ttacaggaat caaatataac atttctaaaa acgaaagcac gttcagtaaa tatggcttcc
     8161 gccaagctgg tcaaagcggc gactatacac tgtatcagaa tgtcaatgca ttgccactag
     8221 catttactgc accgttgtcc gttaatgatg ttgagcagcc agtgacagat aatttgacca
     8281 gtcagacaaa cttgatcaat acattgtctg gtctaaatga acagtatttt acattctata
     8341 cacctacttt gaagaatcag ttgaatacga cgatcacaaa gacaacgact ggcgtcactt
     8401 actcagaaac agcagataac cagccaaaag agctcacttg ggaagtccat gtgcctgcaa
     8461 atacacaagc ttacttaagt ttgttcccaa ccaattttgc gcaattagaa agttctagtg
     8521 cgacgatcac tgtcaatggt acatcgcgaa aaacgcaaat caacatttct ggtcaatact
     8581 atgatcttgg ctattatcct caagagacaa cagtgacatt tactgcaagt ttttatggga
     8641 cgaaagaaat aagtttcatg gagcctaaag tcgttggtct agatgttgta gcctatcaag
     8701 ccgcaatgaa tcagatcaaa gaaaaaggtg tcgaaatgga agccactggc agaaaagctt
     8761 ctgggactgt aaataccgct gaagattcca tgttggtgac aactattcct tatgacaaag
     8821 gatggaaagc taaaattgat gggaaaccag tcaaggtcag caatttcaaa gacgcattct
     8881 tgatgataaa agttccagca ggtaaacata ctgtcacttt cagttacttg cctgaaggat
     8941 ttacgatagg tgcaatcctt tttgtcctat gtattgcatt gtttattctt tatgtctggt
     9001 accttgaccg caatcgcccc ttttatggca cgattgatcc agcaatagaa gaacaaccac
     9061 gaaacacaca cagaagaaga cgtagaaaat aaagaaacga tggaacctgt tcagactgtc
     9121 cgcaccaagg gcaatcaaaa caacgttcca tcgttttttg gttacagaaa aaataggtaa
     9181 caaaaagatg attcacttat caacgcctcg tccacaagtc acgatattca tcaaagatga
     9241 gaagctgttt tttgaaattg ctctttgtga ctcagagtta aatgacaggc tcacgacctc
     9301 tttcttaaac ggtgttcaat cagctgacct tcggtattag ctcaacaaac ggcaaaatat
     9361 gaggagctat tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacgacc
     9421 tcttccttaa acggtgttcc agaagtagct tcttcgaaaa taagccgaaa ttttgcaaaa
     9481 ttttgagaag taattttcgt aaataccttc ttatttcttg aagctgacca cttctatcat
     9541 aacctcttat tcatatttca cttcatacaa ataaagtcct tcagggtgtg ctgtaggtcc
     9601 tgctgcatta cgatcttttt tcgcaataat atctgggata ctatcttcag gcaatcggcc
     9661 attgccaatt ttcaaaagcg ttcctactaa gatccgtatc atcttataca agaagccatt
     9721 tcccctaaat gtaaacaaca attcgtcttt tgtttcattt acctccatct tagcctcgta
     9781 gatcgtacgt attttatcct ctatcgagct tcctgatgca caaaaagagg tgaaatcgtg
     9841 agtccctaat aaatcaggaa gcgcccgctg aattttttcc aaatcaagtg ggtaaggaaa
     9901 ataacttgca taatggcgtc gaaaaggact gcgtggtttc cctatatcca ctcgaaattg
     9961 atacgtcttt tctttgacca aatatcttgc gtggaagtct tctgaaacaa tttctacttg
    10021 tcttacagca atatcctctg gtgactgggt atctagcgcg aaacgcattc gttctaacgg
    10081 acgctcctct ggataatcaa aatggattac ttgcccgatc gcatgtactc cggcatctgt
    10141 ccttcccgaa ccaaagaccg tgatttcttt tccatttgcc attttttgta atgttttttc
    10201 tacttcttct tgaacagtac gcccatttgg ctgtttttgg aatccattaa aatttgttcc
    10261 gtcgtacgca atgattgctt tgtaacgtac catgtttttc tctcctttgt tcctaaaagg
    10321 ttatctggaa taatgatttt tctataa
//
LOCUS       DASJON010000101        10332 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJON010000101 DASJON010000000
VERSION     DASJON010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.101"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..1432)
                     /locus_tag="U3989_001836"
     CDS             complement(41..1432)
                     /locus_tag="U3989_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715285.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="U3989_001837"
     CDS             1689..5078
                     /locus_tag="U3989_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1715286.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="U3989_001838"
     CDS             5081..6502
                     /locus_tag="U3989_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1715287.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="U3989_001839"
     CDS             6499..8376
                     /locus_tag="U3989_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1715288.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="U3989_001840"
     CDS             8468..9310
                     /locus_tag="U3989_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1715289.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..>10332)
                     /locus_tag="U3989_001841"
     CDS             complement(9462..>10332)
                     /locus_tag="U3989_001841"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1715290.1"
                     /translation="IEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATYTS
                     HSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKKMA
                     DLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNKFL
                     IMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQHSE
                     MAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNKQE
                     LMQKGLLKEYRKT"
BASE COUNT         3629 a         1936 c         1932 g         2835 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa ta
//
LOCUS       DASJON010000102        10202 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJON010000102 DASJON010000000
VERSION     DASJON010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.102"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            237..1877
                     /locus_tag="U3989_001842"
     CDS             237..1877
                     /locus_tag="U3989_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN1715291.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="U3989_001843"
     CDS             2031..3353
                     /locus_tag="U3989_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN1715292.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="U3989_001844"
     CDS             complement(3415..3846)
                     /locus_tag="U3989_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715293.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="U3989_001845"
     CDS             complement(3910..4746)
                     /locus_tag="U3989_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN1715294.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="U3989_001846"
     CDS             complement(4936..5238)
                     /locus_tag="U3989_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN1715295.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="U3989_001847"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="U3989_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN1715296.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="U3989_001848"
     CDS             complement(5692..5958)
                     /locus_tag="U3989_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN1715297.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="U3989_001849"
     CDS             complement(6212..6937)
                     /locus_tag="U3989_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1715298.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="U3989_001850"
     tRNA            complement(7303..7373)
                     /locus_tag="U3989_001850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="U3989_001851"
     CDS             7490..8242
                     /locus_tag="U3989_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1715299.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="U3989_001852"
     CDS             8258..8737
                     /locus_tag="U3989_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1715300.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..>10202)
                     /locus_tag="U3989_001853"
     CDS             complement(8988..>10202)
                     /locus_tag="U3989_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN1715301.1"
                     /translation="TIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLFLIILFC
                     LASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENELTDRHPI
                     SILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSNSEFKIK
                     RTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIKLTDLDN
                     KLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEKFRFGWI
                     LQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLLNWTNIN
                     QLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLILSALWI
                     VYYQRTFWKKFE"
BASE COUNT         3097 a         2035 c         1858 g         3212 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gt
//
LOCUS       DASJON010000103        10137 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJON010000103 DASJON010000000
VERSION     DASJON010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.103"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..251
                     /locus_tag="U3989_001854"
     CDS             <1..251
                     /locus_tag="U3989_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1715302.1"
                     /translation="LTEASGGAIDLPTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVD
                     FFLDKKHTVLTIYDAIGIEWWFVSEPKEKNKTDIRCDA"
     gene            460..1485
                     /locus_tag="U3989_001855"
     CDS             460..1485
                     /locus_tag="U3989_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1715303.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            1524..2474
                     /gene="manA"
                     /locus_tag="U3989_001856"
     CDS             1524..2474
                     /gene="manA"
                     /locus_tag="U3989_001856"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN1715304.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            2568..4271
                     /locus_tag="U3989_001857"
     CDS             2568..4271
                     /locus_tag="U3989_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1715305.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            4273..6306
                     /locus_tag="U3989_001858"
     CDS             4273..6306
                     /locus_tag="U3989_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1715306.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            6317..6757
                     /locus_tag="U3989_001859"
     CDS             6317..6757
                     /locus_tag="U3989_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1715307.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6957..8084)
                     /locus_tag="U3989_001860"
     CDS             complement(6957..8084)
                     /locus_tag="U3989_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1715308.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8087..8764)
                     /locus_tag="U3989_001861"
     CDS             complement(8087..8764)
                     /locus_tag="U3989_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715309.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9028..>10137
                     /locus_tag="U3989_001862"
     CDS             9028..>10137
                     /locus_tag="U3989_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1715310.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQ"
BASE COUNT         3359 a         1782 c         2083 g         2913 t
ORIGIN      
        1 gtttgacaga agctagcgga ggcgcaattg atttgcctac agatgaagtg attggacttg
       61 attttctgaa attcatgatc gatactgaaa gcatggaagg actgattgct cacttgcagc
      121 aactatcagt tgatttcttc ttagacaaaa agcacacagt actcacaatc tatgatgcta
      181 taggaatcga atggtggttc gtcagtgagc caaaagaaaa gaataaaact gacattcgat
      241 gcgacgctta actttttctt tcttaacgat tgtcgcatgt ctcgtcattt tatgaacggt
      301 catactatcg cttattctag agatagaaag agaaaatagt ataccggggg aaactgcgcg
      361 gtttttcgcg cagttttttt gtgtaaaaaa ggttgattcc tcatagatta agctttaagc
      421 ttggactgta agaatctgat agaaaagcag gagaaaaaga tgtatccaca cacgaaacag
      481 cttattttga aaaagatgga agaaggattt tatccaggtg tagcagtcgc cttcataaaa
      541 gaaaatcaag tagaaacatt tacagccggc tttgcccaag tcgttcctga aaagcagctg
      601 atgacagaag atctgttgtt tgatgcagcc tcactgacga aagtcgttgc aaccacttca
      661 ttagttttac gtctattaga agatggaaag atagaactcg atcaaccgct tcataaatat
      721 cttcctgaat ttgaaaatca gacaatcact ttgcgtcatt tactgacaca tacttctgat
      781 atcaaaacat ggataccaaa cagagataag ctatctcagg aagaattgat cgccgcttat
      841 ttgaagctag aaccaggtga gcaactagga aaagaagtaa aatatacaga tgccggtacg
      901 attcttcttg ggttgatgct agaggaaatt taccaagaaa ctgcagtgga aatctttcaa
      961 gaacaagtat tggaaccatt aggaatgatg aacagcttat ttcttccttt tccttccaaa
     1021 aaaatcgttc ccacagaaca actgccttcg ggtgaggttt tgcgtggaca aactcatgat
     1081 ccgaaagcaa gagttttggc tgaacatgca ggtaacgcgg gtctctttat tgatttgaaa
     1141 gacagcatta cttttgtcca aatgtatttg aacaagggaa aaatccgtaa tggtcgtttt
     1201 ttagaagaag agacgattga ttctttactg caagatcaaa caccgaaaaa aaatggctat
     1261 cgttctttag gatgggactt aaaaaaggat gtagaagacg gtcatgcgat tctttttcat
     1321 actggttata caggtacgtt tttcttgatt gatataaaaa cacaagaagc gttgatattt
     1381 ttgtcaaatc gcgtacatcc tgtcgatcat cgtgcggagt atctcatatg tcgagatgaa
     1441 attgttgaaa actttttgaa agagaaaacg gtattaccga aataatgcta taatagctat
     1501 gaaaaatgag aggagtttga attatgcagg aacctatgtt tttaaaacca gtttttcaag
     1561 aaaaaatttg gggaggaagt cgtttacgtt cagtctttgg ttttgatatc cctaacgata
     1621 aaatcggaga agattgggcg atcagtgcgc atcctcatgg tgtcagtgta gtagaaaatg
     1681 gcgaattcaa agggaaaaga ttagatgaac tatggtcaga acacaaggaa ttgtttggcc
     1741 atccttcaga accagtgttt ccattattga tcaagatctt agacgcagaa gatgatctat
     1801 ctgtacaagt acatccagat gatgcctatg ggatgaaaca tgaaggtgaa ttaggaaaaa
     1861 ctgaatgctg gtatatcatc gatgctgaac ctggtgcaga aatcatctat ggtcatcatg
     1921 caaagacaag agaagaacta gctgagatga tcaaagatgg gcgctgggat gaccttttga
     1981 agaaagtacc agttaaaaaa ggtgatttct tttatgtacc aagcggaacg atccatgcta
     2041 ttggtaaagg aatcatgatt ttagaaacac agcaaagttc tgacacgact taccgcgtgt
     2101 atgactacga cagaaaagat gcgcaaggac aaacaagaga gctgcatatc caacaatcga
     2161 tcgatgtaac aactgtccct gcgaaaacac cagaattaca aatcaaagaa gtccgaaaag
     2221 ggaattcatc gatcgtcact tatttagaaa ctgagttttt caatgtatat gagtgggata
     2281 ttaaaggaat cacatctttc aaaaaacaag ccccttatac attgatgacg gtaatcgaag
     2341 gagcaggaga ccttgtcgtt gacggaaaaa catatccatt agaaaaagga acaagctgta
     2401 ttattccaaa tggcgtttct gagtgggcgg tccaaggtga actagcaatc attgcgactg
     2461 aaccaggcaa atagttccca gatagctgaa agcagcagac ggtttttcgt ttgctgcttt
     2521 ttctacataa aatccgtcca tcaataaata gaagtatggt gaaaaagatg gaatacaaat
     2581 ggaaagcaaa caacagcagt cgcgtgtcta tgaaacaact gagaaaagct ggacatcact
     2641 taaatgaaat gatcatgcca aatctaagtc tgtttatcgc ttggggaatc ttgtcattag
     2701 caacaaaagg tgtgtctggc gatttgcaca ccacattaga agaggtatgc cattggatga
     2761 tccagctgct tttacccatc ttgatcagtt attcaggcgg gaaatcggta gggggacaaa
     2821 aaggggcagt cgccggtgca attgcttctt tagggttgat cgctcgttcc aatgctccac
     2881 aaatcgtagg agcgatgatt atcgggccgc ttgccggcat cgtatatgaa caatttgttt
     2941 ctagattcca cgtgaaattc aaggctggat atgaaatgct tgtcagcaac ctactagtag
     3001 gactgactgg tagcgttatt tgtataattg gaatcgtttt tattgaacca gttttaaata
     3061 gtgttcactt gttgctcgcc tccgttgtgt cttggctagt cacaatgaat ctgttaccac
     3121 ttgtcagtct gatcttagaa cctctaaaag tactattttt caataatgca atcaatcatg
     3181 gtgtattgac acctttagga atcgaattct cacaaacagt cgggtactct tccttatttt
     3241 tgatggaggc aaatcctgga cctggtttag ggatcttact ttctatcctt tgttttgcag
     3301 aaaaacagga aaaagtaaat gcgagtggag cattgatgat ccatgcgatt ggcgggattc
     3361 acgaaatcta ttttccattt gtcttgctgc gaccgtatct atttttagct gtaatggctg
     3421 gtggggcaag cggcacgttg atatttcaat ggttagatac gggattagct gcacctgtat
     3481 cgcctgggtc actactcgtg atttttgcca atgcgcctta tccacaacta gctggggtaa
     3541 gtatagggat agcaatcagt acatttgtga ccttcatttg tgcagcgata ctattgaaat
     3601 taccaaataa agaaaaagag aacaaaacgg tcaaagaaag gggagaaatc ttggaaccat
     3661 ctatcattca ttccgtcatt tttgcttgcg actcaggtat gggatcaagt gctatgggag
     3721 ccgccctgct gaaaaagaaa ttagcagcag aaaaaatcga tctttctgtc ggatacagct
     3781 ctatctatga attgaataac caagtaaatc agctggttat cgtgcaacat gaactagcgg
     3841 gtcttgcaag agaaaaagta cctcaaagtc aaatctatgt actggatcac tttttggaaa
     3901 cagatatctt gctgtcacga ataaaagaag cgatccaatt tccaaagcgg gaagaaaaaa
     3961 aagagatagc agatatcaca gaacaaaaaa agtcgcctgc taatattgta tttttgtata
     4021 aaaacaacag acgcggctca caaactatgg gcatgacgat tttaagtcaa ttagcaaaaa
     4081 aagcgaaaaa acagtttgtt atctcaaaag aatcaataga aaaaatcaaa atccaagaag
     4141 aaacgattta tatcgcacaa agagaactag ctgtaactga acagctagaa gaaaagatcc
     4201 ctcggctagt tctagttgaa cattgggtag ctacgaatga atacgaaaaa ctcgtgaagg
     4261 gagaaatata agatgtattt atcgcccaga gaaaaacaat tattagcaga atttctcagt
     4321 agcccgtcac ctgtatctat ccaaaaaatg atgaatttat tgaaggtcag taaacggact
     4381 atttaccgcg aattagataa tcttgaagtc tcattgaaat cggtaaatgc ttctttgaaa
     4441 aaagcagcta gaggctcatt tgtcattgag gcatcagaag aagtattaga gcaattaaaa
     4501 gaaaatacag atgggaaaag taatgactta tccactgcta aacgacaaca cgcgatttta
     4561 gccgagttgt tactgacgac agagccgttg agtcttgctc atttcatgga atcatatcgc
     4621 atcagtaata ccacttttta tgcagatatc aaacagttgg aagaaagtat tcgccaactg
     4681 cctttggaaa tcattcggaa ccagggatac gagatcgaag gaccggaaaa ataccgccgg
     4741 cttctgacag cgaatgtatt agaattagaa atcaatgaat atgaactttt tcatagtatt
     4801 tctttcgatg catcactgaa ttattttttt cgattcgttg atccgcaaca tctatctctt
     4861 gcacgcaaag taatcgggga agaattgatc caaaaaaaga ccaatctaag tgatagaaag
     4921 cttgaacatt tggttttgat gctgacattg acgatggagc gtgtgactaa aaatcattta
     4981 ctgacaaatg agacatataa tagtctggca aacaaagaat tactacagac agctaaacga
     5041 ttgttttcac ggatcgcttc tgagacaaaa caactttatc cagtaaatga gatcgtcttt
     5101 tttgcaggat tattaaatga cttcacgaac tcatttgatc agaatttctt tgaagagacg
     5161 ttcgatacac agcttgctta tttagtaaaa caactgattc aagaagtaag tgaagagacg
     5221 aatgtccatt tttatgaaga tgaaactttg tacaaaatgc tgttgactca tttatctggt
     5281 gtgttttcaa gagccgtttt acaagaagag acactgacca atccgatttt agaaaaaatc
     5341 atggttcaat acgaggaagt tgcgaatgca atccgtaaag cagtagagcg gatttttcaa
     5401 gaaaaaaaat tatccgaaga agagatcgct tatatggtcc ttcattttgc gaattcattg
     5461 gaacgaagtc cgaaagaaag caatatcagt attgcaggat tttcgccaag tggattagct
     5521 tctactagca tgttggaact acgattgaaa aggtattttc ccttcatcca acaaattcat
     5581 ttctatcgga ttgctgattt aaaaaaaatc gatttacaag aagaatatga tgttgttatt
     5641 tctacatcta ttttaccagg atacggtgga aaatatattt tagtctcccc gctactgcta
     5701 gaagatgaag tcaagcagct aaaagaagct ttttcgcata ttgagaagaa aaaaaggcaa
     5761 aaaattacgt ataacagcaa taacgagtgg atgatagaag atacttatga ggaaacaatg
     5821 catttcatgg atcaaatcaa tcaattactc ggtcgttttt ttatccaaga actggataat
     5881 cctgagacga tttccgaaac gattcgcagc ttatttcctt attttcctga aggactagta
     5941 acggatgaag aggtggttca tcgccggctg cttaagcgat atgaacaagc acctatcggt
     6001 attcctcata caaatatggg gttattccat acatccagct cattgatttc taaaccgatc
     6061 ttttgtattt tcaatttgaa acatccgttg accattgaag ggatggatag acagccagtc
     6121 gatttgtccc gtatgttagt tatgctggcg ccttcgccaa tccaagaaaa agacgggatt
     6181 cttttaggaa agatcagtgg agcaatcatc atgaatgact tgaacaccga gatcttccgc
     6241 tcaggaaata aagaaatcgt ttatcagttg ttagcgaaaa tcctgattga agaaataaag
     6301 aaatgaaggt gaaaagatga tacagctacc agcagaaaaa atattgctcg atcaatccta
     6361 tcaaaattgg aaagaagcgt tgaccgagat cggccagtgg atgacccaac aagggggaat
     6421 caaaccggca tatatccaat caatgatcga aagacaagaa aaagcgagtg tctatatcgg
     6481 gaatttcgta gccttgccac atgggactga caaagatgaa gaagtagtag aagaaggaat
     6541 ctatttagtc caagtgcctg atggtgtgga ctttggaaca aaagaggaac caaaaatcgc
     6601 gacactctta tttgtagtgg ctttaaaaaa agaacatcag ttagcggtgc tccaagattt
     6661 agcctttttc tgttcagaca tcgaccaagt aatggcgctc agtgatgcga aaaccattga
     6721 agaagtccaa atgatttttg aacatggaca tatataacca atgttcacag taaaaaaagc
     6781 tgccttgcat caagtaataa cttcaaccaa gataaaaata agaggagcta tttttgttgg
     6841 gaggttctta ttactgcgga tgcaagcttt tgaaaactat ttattctaga aaaccgttta
     6901 tttgaaatag gggacggtga caaggttttt gtcaccgccc ctattatttt tgtgttttaa
     6961 tagctctttt ttatatagat agtgcccata cctatgaata ataaaacgat actccatata
     7021 ccaagtgtca agataccatt catgaaagta acctgttctg aagctagtgg cgagaaaata
     7081 tctcctccta tgatcacatt ttgaaaatca acataagaag ttggcaagta atgactgatt
     7141 gattctatcc atccaatcga agaaaacaag cgatactctg ctaagaaaag acaaagaagt
     7201 gtacctaaaa gattgactag agaatgttca aataaagcag atagaagaaa actgattgta
     7261 cttaaaaaga gcacccacaa caacaggaac agaaagctct ggaaaagata ctcatacatc
     7321 ggaaggattc gtatctcctg accaatgatc gttcctacag gataacggaa gtctcccaaa
     7381 ccatttttga ttattactag tatagagacg atcaaaaatg gcatcacgag acttagcata
     7441 gctgcaccat aataaatggt aaaacggatg ctatgtttct ttaccatact atccggtaat
     7501 agattgacta gagagattac ctgctttctt ttttcataag agatgatgga agaaagaaac
     7561 aaggcggtta tacataaaat caaaaaacta gaaatcattc ctgaaaaaat attttcgtag
     7621 taatttgcta aagggagatg cgctggcaag tcaaaaatag aatatctttg gatctgatgt
     7681 tctttcatat aacggagcaa ctcgacacgt ttttgttgtt caatgattgg aattccttgt
     7741 ctttgaccag aaatcagtcg atccagtata tctttttcat aatgataagt tgcttcgacc
     7801 atttgctgga cattccctgt gttttttgca tgtaagattg cttcgatcag tttgttgctt
     7861 ttctctaaac tttccacaaa ttctttctca ttctcatctt ctttcaacgt ttcaatcaaa
     7921 cgttgcatct cttcatagtt atgattcagt tcatcgaatt ctgtataagc aagtttatga
     7981 tcaaaaaaaa gtgggaatgc aaagagcacg accaacaata gaggaaataa aaagtttact
     8041 ttgtttttta gtaagagatt gcgttcaaat gtaagtaaag acatagttac acctcatata
     8101 atttacgata aatagctaaa atctcttcca tatttgagga ttctgtgacg atttctccat
     8161 tttttagaaa acatgcttta gaacaaatcg attggatatt cgcgatagcg tgggaagaaa
     8221 gaagaatcgt cttcccctct ttctgccatt gtctgatgag gctattcaag atatcaatac
     8281 ttcctgggtc taatccgttc aacggttcat cgaataacat gacagatgaa tcactgatca
     8341 agtaagcagc aagcaagaca tgttgtttca ttcctagaga catttttcca atcggcaaag
     8401 taaggtactc agtcatcttc aatcgttgga aaacagtttg gcattctact ttgctctgcc
     8461 aatagtattt gatcaaagtc aaataatcaa cagccgatag atagtcggat aactgattcg
     8521 ttgattctag aaaaaagaaa gaccggcgat aatctgtata agcagtagga gagatgttgt
     8581 gaagggacaa ttgatcataa gttgttggca gcagttgggc aatgccattc agcaaagttg
     8641 ttttgccaaa tccgttcggg gcaatcaaag cagctacttc tcctgattct actaaaagat
     8701 tcaattgtcg aatcacacgt tttttaccac gtttcatgtt catgtttttg atcagtaatg
     8761 tcatagatag gcctccaata taaaatttaa taacataata gaatggaaaa catctaaatt
     8821 aaatgctaac aaaagttatt gtttgcatat tataaaaaaa ttaaaataac agttttattt
     8881 aaaattgaag ttttctgata ccgaaatctt tttattttgc tgacatactc aaacctttgg
     8941 cacaaagttt cgtgccaaag tacgactatt tctcttttta gtaagcggat acaattagag
     9001 tatcaaaagg agagagggag agacaaaatg gaacggacga ttcccaaaaa caacatttcg
     9061 ctaaaagcga gggtacaaaa attaggcagt actttatcaa gcatggtcat gccaaatatt
     9121 ggagcactga ttgcctgggg tgtattaacg gctctattta tcccagatgg ttatttacca
     9181 aatgaggctt tggctaccat ggtaagccca atgctaactt atctgattcc tttattgatc
     9241 ggttatactg gcggaaaagt aattgctggt gaccgcggat cagttgttgg ggcaattgca
     9301 acgatgggtg tgattgtcgg aacagatatt ccaatgatgc taggcgcgat gatcatggga
     9361 cctcttggtg gatatgccat caagaaattt gatcagctat tccaaaaacg gatcaaatcc
     9421 ggttttgaaa tgctagtcaa taatttttca gcaggtctta tcggttttgg attagcgtta
     9481 ttaggttttt ctgcgattgg tccagttgtc gatgcattga ctcaagcaat ggcaaaaggt
     9541 gtagagatca ttttatctgc ccatttgatt ccattgacaa gtatttttat tgaaccagca
     9601 aaaatcttat ttttgaataa tgcaatcaat catgggattt tgacaccatt gggaactgaa
     9661 caagtagcag aggctggaaa atctattcta ttcttgcttg aagcaaatcc tggtccagga
     9721 cttggcgtat tacttgcttt tactttgttt ggcaaaggag cagcaaaatc ttcagcacca
     9781 ggagccatga tcattcactt tttaggcggg atccatgaaa tctattttcc ttacgttatg
     9841 atgaaaccgt tattattctt atcagttata ggcggaggaa tgtcaggaag tttcattttc
     9901 caattgatgg atgctggact aagagcgcca gcttctccag ggtcgatcat tgcgattcta
     9961 gcgatgacac cgttcaattc ttaccttcct gttattttgg gcgtgattgt tgccgcagct
    10021 gtttcgtttg gtatttctgc agtaattttg aaagcggatg cgaaagatac aaatgaagac
    10081 ttcgaaaaat ctgttcaaga gacaaaggca gctaagcaag cttccaaagg tgcccaa
//
LOCUS       DASJON010000104        10136 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJON010000104 DASJON010000000
VERSION     DASJON010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.104"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..53)
                     /locus_tag="U3989_001863"
     CDS             complement(<1..53)
                     /locus_tag="U3989_001863"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN1715311.1"
                     /translation="MLVFQMRNVDKTSTVLK"
     gene            complement(102..341)
                     /locus_tag="U3989_001864"
     CDS             complement(102..341)
                     /locus_tag="U3989_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN1715312.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(475..1692)
                     /locus_tag="U3989_001865"
                     /pseudo
     CDS             complement(475..1692)
                     /locus_tag="U3989_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            2183..3121
                     /locus_tag="U3989_001866"
     CDS             2183..3121
                     /locus_tag="U3989_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1715313.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            3251..3898
                     /locus_tag="U3989_001867"
     CDS             3251..3898
                     /locus_tag="U3989_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HEN1715314.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(4591..5529)
                     /locus_tag="U3989_001868"
                     /pseudo
     CDS             complement(4591..5529)
                     /locus_tag="U3989_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5529..6146)
                     /locus_tag="U3989_001869"
     CDS             complement(5529..6146)
                     /locus_tag="U3989_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1715315.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(6160..6330)
                     /locus_tag="U3989_001870"
     CDS             complement(6160..6330)
                     /locus_tag="U3989_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715316.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(6678..8168)
                     /locus_tag="U3989_001871"
     CDS             complement(6678..8168)
                     /locus_tag="U3989_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1715317.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(8211..8510)
                     /locus_tag="U3989_001872"
     CDS             complement(8211..8510)
                     /locus_tag="U3989_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715318.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8876..9160)
                     /locus_tag="U3989_001873"
     CDS             complement(8876..9160)
                     /locus_tag="U3989_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715319.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(9679..10122)
                     /gene="vanZ"
                     /locus_tag="U3989_001874"
     CDS             complement(9679..10122)
                     /gene="vanZ"
                     /locus_tag="U3989_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN1715320.1"
                     /translation="MTLIWLVLFKLQYNILSVFNYHQRSLNLTPFTATGNFREMIDNV
                     IIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGATDITDVITNTVGGF
                     LGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINYV"
BASE COUNT         3125 a         1738 c         1558 g         3715 t
ORIGIN      
        1 tgtttcaaaa cagtagatgt tttatctaca ttacgcattt ggaataccaa catgacgaat
       61 ccctccttct taattacaaa tttttagcat ctaatttaac ttcaattcct attatacaaa
      121 attttaagat aatgcactat caacacactc ttaagtttgc ttctaagtct tatttccata
      181 acttctttta cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtca
      241 gcacgaacac gaaccgtctt atctcccatt atatcttttt ttgcactgat tggtgtatca
      301 tttcgttttt ctttttgtgc gcctaaattt cccacaatca ctcacttctt tctatttctt
      361 cttattctta ttttatcatc aacaatcaca aatcacttgt gatttgtgat aagtgatttg
      421 tgataagtga tttgtgatta atatataaaa gccctcttta aagggctttt atgtttattt
      481 tgagaaagat ataaaatcaa tatatccctt ttccccaatt tttacaacgg cattgtaggg
      541 ctttttctct ttgtttttga ttccttttac cagggtttct tttccctcca gtaattcttt
      601 tacattcgtt ttggttagtt ttttctttct aaaatgttca gctaaagtaa acttacattc
      661 aggataattt gaacaaccat aaaacgattt ttttaataca atattgttgc cacacttagg
      721 acattttcct acaatacttt tttctgcttc tttttctttc tgttcctggt aatcagaaaa
      781 atttagtttt tctatatcgt taggtacagc ttccagtaaa tgaacaatga attttttgat
      841 attcgtaata aagttctctt gattgccttc tcttttaccg atttttttta aatacgtttc
      901 ccatttagcc gtcatttcag cactcgttaa aaggtgctga ctttcaactg cctggcacaa
      961 taattttcct ttttcagtta caacaagctt attcttaatc acttggatat attctttttg
     1021 ttttaacgct tcaataatac ttgctcttgt cgcttctgtt ccaatcccct caacttcttg
     1081 taaaatcttg attgcttctt catcatcaac cgttttattc gccgttttca tagcagttaa
     1141 taatgtaccc tctgtaaaag cttttggcgg ttgcgtttct ttttcaacac tcttaacacc
     1201 aacttcggca cgttcgccaa tgataacgag tggtaacgtt tgaacgtcct ctttttcttc
     1261 tttggtttgt tgtttgaaaa gaattttcca cccttcttgc tttggtgtct ttccgatcga
     1321 ttgaaacagc aaatcagcta ctttcgtttg tatcttggtt tcttcataca agtaatcagg
     1381 taaaaacatg gcaacggtcg ttttaacgac taaagcataa atttttcgtt gtaaatcgtc
     1441 cattttcgct aatgcagatt cggtaggtac ttgttttgtt gggataatgg cgtggtgttc
     1501 ctgtacctta ctaccgtcca cataacgctt tctaggctct gtttgaacca tttcaagatc
     1561 aagtcctaaa aaaccgctat atttgccaaa attcgctttt aaataagcaa attcgttctc
     1621 tgtaataaat ggtgtatctg ttcttggata actcaataat tttgcttcat acagtccttg
     1681 catagctttt aaacgtaaaa ttttgcgttc taagacgttt taaatatatc tgcgtatatt
     1741 tttatgtgtt ttatcttttc gtcggcaaat gcgttaaaat ttgcgaaata aaatagctca
     1801 agggcgaacg cagtgagtgc atttaccctt gatctatttt tttgagtaaa ttagcttttg
     1861 aaaccgacgg aaaggaaaca caaaactttt cagaggcaag gggtttttaa ggggcgcttt
     1921 tagccccttg aatttatttg ctttaagata tatttagttt taaaatagtt ttaacgaaaa
     1981 aaagacataa aaaaagacga gcaacgctac caacgttctc gcccggaata acaaaccaca
     2041 cttgttattc attcgatgat tagtatatca atttttgatt ttgaatacaa aacaaaatgg
     2101 acgaatggat acaggcgaaa tttattttgc gattccactc gtcctttttt cgttccaaaa
     2161 attattttag gagcgataaa aaatgaagaa aattgatgaa aaattagctg cagaaatctc
     2221 actaaaaaaa ccggccgtct ctaatagccg gttaagtaat agccggttaa gtggtcaaac
     2281 tttgggaaaa tctcaacccg cattaagttt tgatgccatg acaatcgttg gaaatttgaa
     2341 caaaactaat gctaaaaagc tatctgactt tatgagtgta gagccacaaa tacgactttg
     2401 ggatatactt caaacaaagt ttaaagctaa ggcacttcaa gaaaaagttt atatcgaata
     2461 tgacaaagta aaagcagata cttgggatag acgtaatatg cgtgttgaat ttaatcccaa
     2521 taaactcaca catgaagaaa tgatttggtt aaaacaaaat attatcgact acatggaaga
     2581 tgacggtttt acaagattag acttagcttt tgattttgaa gatgatttga gcgattacta
     2641 tgcaatgact gataaagcag ttaagaaaac tgttttttat ggtcgtaatg gcaagccaga
     2701 aacaaaatat tttggtgtcc gtgatagtga tagatttatt agaatttata ataaaaaaca
     2761 agaacgtaaa gataacgcag atgttgaagt tgtgtctgaa catttatggc gtgtagaagt
     2821 tgaattaaaa agagatatgg ttgattactg gaatgattgt tttaatgatt tacacatctt
     2881 gaaacctgcg tgggctactt tagaaaaaat taatgagcaa gctatggttt atactttgtt
     2941 gcatgaagaa agtatgtggg gaaagctaag taagaatact aagactaaat ttaaaaaatt
     3001 gattagagaa atatctccaa ttgatttaac ggaattaatg aaatcgactt taaaagcgaa
     3061 cgaaaaacaa ttgcaaaagc agattgattt ttggcaacgt gaatttaggt tttggaagta
     3121 aaataagttt tatttgataa aaattgctaa ttcagtataa ttaatattta cgaggtgaca
     3181 taacgtatga aaaaatcaga ggattattcc tcctaaatat aaaaatttaa aatttaggag
     3241 gaagttatat atgactttta atattattga attagaaaat tgggatagaa aagaatattt
     3301 tgaacactat tttaatcagc aaactactta tagcattact aaagaaattg atattacttt
     3361 gtttaaagat atgataaaaa agaaaggata tgaaatttat ccctctttaa tttatgcaat
     3421 tatggaagtt gtaaataaaa ataaagtgtt tagaacagga attaatagtg agaataaatt
     3481 aggttattgg gataagttaa atcctttgta tacagttttt aataagcaaa ctgaaaaatt
     3541 tactaacatt tggactgaat ctgataaaaa cttcatttct ttttataata attataaaaa
     3601 tgacttgctt gaatataaag ataaagaaga aatgtttcct aaaaaaccga tacctgaaaa
     3661 caccataccg atttcaatga ttccttggat tgattttagt tcatttaatt taaatattgg
     3721 taacaatagc agctttttat tgcctattat tacgataggt aaattttata gtgagaataa
     3781 taaaatttat ataccagttg ctctgcaact tcatcattct gtatgtgatg gttaccatgc
     3841 ttcactattt atgaatgaat ttcaagatat aattcatagg gtagatgatt ggatttagtt
     3901 tttagatttt gaaagtgaat ttaattttat acacgtaagt gatcataaaa tttatgaacg
     3961 tataacaacc acattttttg gttgcttgtg gttttgattt tgaatttggt tttgaactta
     4021 tggactgatt tattcagtcc attttttgtg cttgcacaaa aactagcctc gcagagcaca
     4081 cgcattaatg acttatgaaa cgtagtaaat aagtctagtg tgttatactt tacttggaag
     4141 atgcaccgaa taaaaaatat tgaagaacaa ctagcaaaag attttaaaga gttattttat
     4201 tttaagtctt tataacatga gtgaagcgaa tttttaaatt tcgatagaaa tttttacatc
     4261 aaaaagcccc ctgtcaaaat tgacgaaggg ggttttttgg cgcacgcttt tcgttagaaa
     4321 tatacaagat tgaaaatcgt gtataagtgc gccctttgtt ttgaacttag cacgttacat
     4381 caatttttta aaatgatgta taagtgcgcc cttttaaatt ttgagtgatt atattttttg
     4441 agttagaaaa agggattggg aaaatttccc aaaataattt aaaaaataag caaaaatttt
     4501 cgatagagaa tgtgctattt tttgtcaaag gtgtatacct tgactgtgct tgctgttaca
     4561 ttaagtttat ttttaagtta ttaaaaaaga aatagctttt aaagtttggc tcgctgtcgc
     4621 tttataaagc tgattgactt ttgattgcaa actacttaaa gaaaacaaac tcggactatt
     4681 cgttttcttc tctttggttt gaacatcagc aattatcccc tcttgattgc ctattttagc
     4741 ttgtttagaa gaaacaaaag ctaaaagctc ctcttgggtt ttaaaacgct gtgtggggct
     4801 tagaacgccc ttaaacgacc cttggtttac ttttatacta gcttccacct cgaaaaaagg
     4861 ttctttttta aaattctcta tggcttcctg gcgctgaaaa ataaggtata aggtgggcgt
     4921 ttgaacacgt cctagtgaaa atgtaccttg tacgcccttc tgttgtaaat ttaacgtata
     4981 caaagggctt gcgttcatgc cgatcaacca atcggcaatt tggcgtgttt gcgcttcttg
     5041 ataaaaggga tagtaattca ttccaggttg caaattttga aaaccgcttc ggattacatc
     5101 tttttctaag ctattgatcc atagtctttt aaatgtttta tcttttgaaa aggcatttgc
     5161 tttatggata atcgaccagg cgatattttc accttctctg tcgctatctg ttgcaacaat
     5221 aattgtattt gcctttttga gaagttctgc aacaatttta aactgctttc ccttatcttt
     5281 tgcaacttca aaatcgtatc gatcaggaaa aatcggcaaa gattcaagtt tccaattttg
     5341 ccacttttcg tcataatgac ctggttctgc taattccact aaatgcccaa aaccaaaggt
     5401 gataaacgtt tcatctgtaa atagtgggtc tttgatctca aaataaccgt cttttttggt
     5461 gctttgtttt aaagcacttg cgtaggctaa tgcctggctt ggtttttcag ctaaaataac
     5521 cgtactcatt aactatccct cttttcattg ttttttcttt gatcgactgt cacgttatat
     5581 cttgctcgat accttctaaa cgttcggcga ttgattccag tttgttcttc aacttcttta
     5641 tcggataaac cattcaaaaa caaatcgaaa gcatgcttta aacgtggatc attttcttta
     5701 aatttatgct gacgaccttt gaatttgcct tttttcttag caatttcgat tccttgtgcc
     5761 tgacgttcct taattttttt tcgttctgat tctgcttgat acttgtacaa ttcaatgaca
     5821 aggctattaa tcaaacgcct taaattttca tcttcaatac cattcattga gggtaaattt
     5881 aagacttcca gggttgcccc cttaatttga atttgattca tcaattctgt taattcttta
     5941 ttatttcgtc ctaatcgatc taattcagta acaataacaa tatccccttc acgaatatag
     6001 ttaagcatag cttgtaattg tgggcgttcg accgattgac cgcttaattt gtctgaaaag
     6061 accttagaaa cgccctgtaa cgcttgtaat tgccgatcta agttctgttc tttgctactg
     6121 acacgtgcat aaccaatttt agccattttc aaccaacctc taaaattctc tcggttgcaa
     6181 taaccaatca gcaatatcta ctttttcaat ttcaaattgc ttatcagaaa ttgtcttttc
     6241 gtaagcgata aaatcttgcg catattgttg ctcattaaaa atagccacca cttcgtcatt
     6301 ttctaaaact cgataaataa attttttcat tttactcctc ctattatgcc caacttaaat
     6361 gacctattca ccaagtcaat tatactgcta aaatcatatt aggacaaata ggtatactct
     6421 aatgacctat aaaagataga aaattaaaaa atcaagtgtt cgcttcgctc tcactgcccc
     6481 tcgacgtttt agtagccttt ccctcacttc gttcagtcca agccaactaa aagttttcgg
     6541 gctactctct ccttctcccc ctaataatta attaaaatct tactctgtat atttctgcta
     6601 atcattcgct aaacagcaaa gaaaaaacaa acacgtatca tagatataaa tgtaatggca
     6661 tagtgcgggt tttattttca gcctgtatca tagctaaaca aatcgagttg tgtgtccgtt
     6721 ttagggcgtt ctgctagctt gtttaaagtc tcttgaatga atgtatgctc taagtcaaaa
     6781 gaatttgtca gcgcctttat atagctttct ttttcttctt tttttacttt aatgatcgat
     6841 agcaacaatg atttaacact agcaagttga atgccaccat ttcttcctgg tttaatctta
     6901 aagaaaattt cctgattcgc cttcagtacc ttcagcaatt tatctaatgt ccgttcagga
     6961 atgcctagca cttctctaat ctcttttttg gtcgtcacta aataaggctt gtatacatcg
     7021 cttttttcgc taatataagc cattaaatct tctttccatt ctgacaaatg aacacgttga
     7081 cgttcgcttc tttttttctt gaatttaaac cacccttgac ggacaaataa atctttactg
     7141 gttaaatcac ttgataccca agctttgcaa agaatggtaa tgtattccct attagcccct
     7201 tgatagtttt ctgaataggc acttctaaca attttgatta cttctttttc ttctaagggt
     7261 tgatctaatc gattattaaa ctcaaacata ttatattcgc acgtttcgat tgaatagcct
     7321 gaactaaagt aggctaaaga gagggtaaac atgacgttat tacgccctat taaacccttt
     7381 tctcctgaaa atttcgtttc gtgcaataag agattaaacc agggttcatc tacttgtttt
     7441 ttgccttctg taccgcttaa aaccgttaga cttgaacgag taaagccctt attatctgtt
     7501 tgtttgaaag accaatcttg ccattctttg aaagaataac ggtaattagg atcaaaaaat
     7561 tctacattgt ccgttcttgg tatgcgagca ataccaaaat gattacacgt tagatcaact
     7621 ggcaaagact ttccaaaata ttctcggata ttttgcgaaa ttattttggc tgctttgaca
     7681 gatttaaatt ctgattttga agtcacatag actggcgttt ctaaaacaaa atatgcttga
     7741 taacctttat cagatttgat aatcatagta ggcataaaac ctaaatcaat agcggttgtt
     7801 aaaatatcgc ttgctgaaat agtttctttt gccgtgtgaa tatcaaaatc aataaagaag
     7861 gtattgattt gtcttaaatt gttttcagaa tgtcctttcg tgtatgaacg gttttcgtct
     7921 gcatacgttc cataacgata aacgttgggt gtccaatgtg taaatgtatc ttgattttct
     7981 tgaatcgctt cctcggaagt cagaacaaca ccacgaccgc caatcatgct tgattttgag
     8041 cgatacgcaa aaatagcccc tttgctttta cctggcttgg tagtgattga gcgaatttta
     8101 ctatttttaa atttgtactt taacaagccg tcatgaagca cagtttctac aacaaaaggg
     8161 atattcattc agctgttctc ctttcctata aatcctataa aataggttgt ttaattaact
     8221 tggtttgctt tttcattcaa ctgtttcaat attgcatgtt ttgaaaaaga tttttttcct
     8281 ttataagtca atttttttcc actaatcgaa taaattattt tgttattttc tattaactta
     8341 tatatataat cttccccctc cgaagaaaaa tacttatctg attttgtttc taagtagata
     8401 tttctctttt ctaactcttt cttaaacgtt tctagtgtat agatatttgc taattttctt
     8461 atctccaata aactattttt tatataagtt ttacattcat catgattcat acaaactcca
     8521 ccttctataa atgaatacaa aaaaagcaat caaacgattt ccgattgatt gcttaacaat
     8581 tcttaaattc agtagcttag atacttgaaa actctctgat ttccctatat aatgatagta
     8641 cggttatata ccgtcttcaa acaaagttaa ttaaataact tcttacgagg gaagagttca
     8701 tctgactaac tgataagcgt tggtttggca atcttatcgg gctatgcatt tataaaatgt
     8761 cgtcaaacat tttataaatg tgtcatggct cttttttcgt ttctattcag ttcgttgttt
     8821 cgttatatct agtataccgc ttttaaaaaa aataagcaac gatttcgtgc attattcaca
     8881 cgaagtcatt gcttttttct tcttccattt ctaaatccaa tgttacttgt tctgattctg
     8941 tttctggttc tggttctgtt ggctcatttg ggattaaatc cactactagc gttgagttag
     9001 ttaactttgc aatttgttct agtgttttta tggttggatc tgattttcct gattctattc
     9061 gtgaataatt tgatctactc atttctaatt cttgggagaa tttttctttt gatttggtac
     9121 ctctcatctt ttttaagctt tctctaaatg ctagttccaa gtttctcccc cctctttttg
     9181 tgtaataaaa ttcacgcttt atttaaatta tagcttttaa tatcttaata aacaagtgat
     9241 taatcacttg tgatttgtga ttattccaaa agtaggtaat ttcaccaacc tttttcaaaa
     9301 gtttttatcg acgattattg gactggatcg tgtttcctaa agcgattcta agcgcatgtt
     9361 taattttaaa gtgggtaatt ctctttaaaa cgtcctacaa agattcaaaa aaagcatggc
     9421 tgctagtggg ggtaccgtca ggaaaatgcg gatttacaac gttaaggaaa tttattaaag
     9481 agaccaattc ctcagcacta aaattgataa ttgggctttc tttgttacta tagtgttagg
     9541 taagcttttt actttatcac cggttttccc ctcacttcac acctacgggc gagtttcccc
     9601 acatcccgtt ttccatctaa tccaattcat tcaatatctt tatgtgtatg aaagatcaga
     9661 ttgcttgatt tagacatctt acacgtaatt tattcttaaa tgggtacggt aaacgagcaa
     9721 taatacgagc aaaagtatac ctacaaaaat aataactctg tctaattttt tttgattcat
     9781 atgcttattg cttaaaccat ataatttcag tccaagaaag cctccaacag tatttgtaat
     9841 tacatctgtt atgtctgtcg ctccaatagc gaagataaat tgaattattt caaaagtaag
     9901 acttaaaacc agtacaaaag caaacttagg taaaaatccg atttctttaa aattgacatt
     9961 caaaagcaag ccaaatggaa taaagattat aacattatct atcatctctc tgaaattccc
    10021 agtagcagta aatggagtca agttaagact tctttgatga taattaaata ctgataaaat
    10081 attgtattgt aatttgaata acactaacca gattagtgtc actaaatata aagcta
//
LOCUS       DASJON010000105        10129 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJON010000105 DASJON010000000
VERSION     DASJON010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.105"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..310
                     /locus_tag="U3989_001875"
     CDS             137..310
                     /locus_tag="U3989_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715321.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="U3989_001876"
     CDS             950..1423
                     /locus_tag="U3989_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN1715322.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="U3989_001877"
     CDS             1432..1659
                     /locus_tag="U3989_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715323.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="U3989_001878"
     CDS             2295..2666
                     /locus_tag="U3989_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715324.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="U3989_001879"
     CDS             complement(2922..3164)
                     /locus_tag="U3989_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1715325.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="U3989_001880"
     CDS             complement(3196..4098)
                     /locus_tag="U3989_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1715326.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="U3989_001881"
     CDS             complement(4111..4299)
                     /locus_tag="U3989_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1715327.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="U3989_001882"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="U3989_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1715328.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="U3989_001883"
     CDS             complement(4914..5807)
                     /locus_tag="U3989_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1715329.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6808)
                     /locus_tag="U3989_001884"
     CDS             complement(5885..6808)
                     /locus_tag="U3989_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1715330.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="U3989_001885"
     CDS             complement(6857..7207)
                     /locus_tag="U3989_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1715331.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="U3989_001886"
     CDS             complement(7240..8142)
                     /locus_tag="U3989_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1715332.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="U3989_001887"
     CDS             complement(8135..8992)
                     /locus_tag="U3989_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1715333.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..>10129
                     /locus_tag="U3989_001888"
     CDS             9326..>10129
                     /locus_tag="U3989_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1715334.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNF"
BASE COUNT         2913 a         1897 c         1690 g         3629 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttc
//
LOCUS       DASJON010000106        10090 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJON010000106 DASJON010000000
VERSION     DASJON010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.106"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="U3989_001889"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="U3989_001889"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN1715335.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="U3989_001890"
     CDS             complement(1171..1866)
                     /locus_tag="U3989_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN1715336.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="U3989_001891"
     CDS             complement(1879..2595)
                     /locus_tag="U3989_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN1715337.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="U3989_001892"
     CDS             2925..3533
                     /locus_tag="U3989_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN1715338.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="U3989_001893"
     CDS             3858..4181
                     /locus_tag="U3989_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN1715339.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="U3989_001894"
     CDS             4363..5733
                     /locus_tag="U3989_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN1715340.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="U3989_001895"
     CDS             complement(5863..7164)
                     /locus_tag="U3989_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1715341.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9476)
                     /locus_tag="U3989_001896"
     CDS             complement(7518..9476)
                     /locus_tag="U3989_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN1715342.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="U3989_001897"
     CDS             complement(9653..9919)
                     /locus_tag="U3989_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1715343.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2957 a         2095 c         1830 g         3208 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc
//
LOCUS       DASJON010000107        10047 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJON010000107 DASJON010000000
VERSION     DASJON010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.107"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..946
                     /gene="recF"
                     /locus_tag="U3989_001898"
     CDS             <1..946
                     /gene="recF"
                     /locus_tag="U3989_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN1715344.1"
                     /translation="IGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQKKL
                     STYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQRNQ
                     YLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQKEQ
                     LEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQKN
                     VQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETIEG
                     KVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            943..2889
                     /gene="gyrB"
                     /locus_tag="U3989_001899"
     CDS             943..2889
                     /gene="gyrB"
                     /locus_tag="U3989_001899"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN1715345.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            2909..5380
                     /gene="gyrA"
                     /locus_tag="U3989_001900"
     CDS             2909..5380
                     /gene="gyrA"
                     /locus_tag="U3989_001900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN1715346.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            5616..5915
                     /locus_tag="U3989_001901"
     CDS             5616..5915
                     /locus_tag="U3989_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN1715347.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            5965..6477
                     /gene="ssb"
                     /locus_tag="U3989_001902"
     CDS             5965..6477
                     /gene="ssb"
                     /locus_tag="U3989_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1715348.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6503..6739
                     /locus_tag="U3989_001903"
     CDS             6503..6739
                     /locus_tag="U3989_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN1715349.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            6882..8855
                     /locus_tag="U3989_001904"
     CDS             6882..8855
                     /locus_tag="U3989_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN1715350.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            8861..9313
                     /gene="rplI"
                     /locus_tag="U3989_001905"
     CDS             8861..9313
                     /gene="rplI"
                     /locus_tag="U3989_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN1715351.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            9587..>10047
                     /locus_tag="U3989_001906"
     CDS             9587..>10047
                     /locus_tag="U3989_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN1715352.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEK"
BASE COUNT         3360 a         1821 c         2223 g         2643 t
ORIGIN      
        1 gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac
       61 tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga
      121 acaaaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga
      181 tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg
      241 ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag
      301 aaaccagtat ttaaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat
      361 tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt
      421 caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca
      481 gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat
      541 ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac
      601 aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca
      661 gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat
      721 cgatttgatt aaagaagaga cgggggaata tccaatcctt ttgttagatg atgtgatgag
      781 cgagctggat gatagtcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac
      841 gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat
      901 tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt
      961 ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa
     1021 gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac
     1081 cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa
     1141 atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata
     1201 cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta
     1261 catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta
     1321 ggatcttctg ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga
     1381 aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa
     1441 caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag
     1501 acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac
     1561 cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac
     1621 cattatgacg gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt
     1681 tttccagaac ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg
     1741 caatatacag acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat
     1801 gaaggcggaa cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat
     1861 gcccgaaaac aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga
     1921 gaaggattga cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca
     1981 aaaacaaaat tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat
     2041 ttcatgaaat tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta
     2101 ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt
     2161 gctttagaga ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa
     2221 aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt
     2281 aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca
     2341 agcatggaca agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca
     2401 gggtttggag aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact
     2461 gatgccgatg tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac
     2521 atgcgtccta ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta
     2581 aaacaaggga agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa
     2641 caagtcgttg cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt
     2701 ggagaaatgg atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg
     2761 gcccgcgtca gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg
     2821 gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta
     2881 gatatttaat catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga
     2941 tgtcaatcta accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt
     3001 cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata
     3061 cggcatgaac gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt
     3121 cggagatgtc atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt
     3181 acggatggca cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg
     3241 ttctgttgac ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat
     3301 cgctacagaa atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga
     3361 tgatacagaa aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg
     3421 aacaacgggg atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt
     3481 agtagcggct atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga
     3541 agtattacct ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg
     3601 aagagcgtat gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga
     3661 gatgccaaat ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc
     3721 aaaattgatc gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga
     3781 tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc
     3841 cagcgcatct gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg
     3901 attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct
     3961 tgaaaactat atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga
     4021 aaaagcagaa gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga
     4081 tgaaatcatt gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat
     4141 cgaacggttc gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg
     4201 attgaccggt ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat
     4261 tgaagattta actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga
     4321 attagcagac ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga
     4381 ggtcttaagt ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac
     4441 aaacaatggt tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg
     4501 acgtggtgtc caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg
     4561 ttctacccat gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg
     4621 atacgaaatt ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg
     4681 tatcgattcg agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg
     4741 ccactatctc ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt
     4801 tgctaacatc cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt
     4861 caatgtgcta ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc
     4921 tgtcactttc aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg
     4981 aatacgcctg cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga
     5041 agtattagtg atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt
     5101 gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact
     5161 agctggattg accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt
     5221 cattatccgt ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg
     5281 tttgatgaag atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc
     5341 tgcagaacaa acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta
     5401 agctagcaag aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt
     5461 gaatttctct taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt
     5521 gtataatcag agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac
     5581 ctaagagccg aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca
     5641 tgtacattat tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg
     5701 atgcaatctt gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc
     5761 gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt
     5821 ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata
     5881 tcattcgtca catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga
     5941 aaacgatgag aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg
     6001 aaagatccag atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct
     6061 gtgaatcgta attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg
     6121 atttggcgca aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt
     6181 gttactggac gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg
     6241 acagaagtag tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc
     6301 caacaaagcg gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat
     6361 aactctaatc aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct
     6421 gacccattcg gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg
     6481 aatttgatag gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa
     6541 agtagactac attgctgcta accatattga atatatcgat tataaagata tcgaattact
     6601 aaaaagattt atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc
     6661 taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc
     6721 tttcgttagc gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta
     6781 ttcaattcct ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa
     6841 gttatggtaa aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc
     6901 atttatcccc gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct
     6961 taattttacc atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg
     7021 ttcgctattg gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc
     7081 aggcttcgac tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag
     7141 tgattgctta tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa
     7201 tcaaagatca aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag
     7261 aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag
     7321 taaagggtgt tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga
     7381 cgaacagtca gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata
     7441 aattggatga caaagagatt tcttatttga actctttcgt cactacacta gtttctgact
     7501 ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag
     7561 cacagatgat cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc
     7621 gagcagaagc tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct
     7681 caagaaccct cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc
     7741 gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg
     7801 gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg
     7861 ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg
     7921 atatggatgc tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa
     7981 aagcttacat cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag
     8041 aaatccataa gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc
     8101 tgaaaaaaga agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct
     8161 ctcagggcct atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg
     8221 aagagtttcc aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag
     8281 agctagtttc tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg
     8341 aggccacatt attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa
     8401 ctgcacgaac ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag
     8461 tacaatattt attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg
     8521 gaagcgacta tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata
     8581 atagtgttat agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag
     8641 ctttcgtcat tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca
     8701 cgatcaacgt ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag
     8761 ctactcaact ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca
     8821 aagaaaatat gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca
     8881 agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca
     8941 aaattatcta atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt
     9001 agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa
     9061 gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga
     9121 agacagccgt ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca
     9181 atacaaaatc aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg
     9241 ctacacaaac gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt
     9301 cgtacaagaa taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa
     9361 aaagacaaac cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa
     9421 tcgatttcag attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg
     9481 agtataataa aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc
     9541 tcattttctt gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg
     9601 gcaggatcga atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt
     9661 tcttgacgca gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg
     9721 tcgtggccat caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat
     9781 tgacgtggtt actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg
     9841 tttaagttat ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta
     9901 tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat
     9961 cgttacgaaa ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg
    10021 cagtatcttg gaggtttcgg aaaaaag
//
LOCUS       DASJON010000108        10014 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJON010000108 DASJON010000000
VERSION     DASJON010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.108"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            186..2021
                     /gene="tdc"
                     /locus_tag="U3989_001907"
     CDS             186..2021
                     /gene="tdc"
                     /locus_tag="U3989_001907"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1715353.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2326..3804
                     /locus_tag="U3989_001908"
     CDS             2326..3804
                     /locus_tag="U3989_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1715354.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3770..5101
                     /locus_tag="U3989_001909"
     CDS             3770..5101
                     /locus_tag="U3989_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1715355.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5215..7767
                     /locus_tag="U3989_001910"
     CDS             5215..7767
                     /locus_tag="U3989_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN1715356.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7774..9213)
                     /locus_tag="U3989_001911"
     CDS             complement(7774..9213)
                     /locus_tag="U3989_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715357.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9559..9921
                     /locus_tag="U3989_001912"
     CDS             9559..9921
                     /locus_tag="U3989_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN1715358.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3187 a         1650 c         2129 g         3048 t
ORIGIN      
        1 ttgttcgctt ttttgcgttg aattatcaga aaaatccgac tttatttatt tttgtttgta
       61 aaaatctacg tggatttgat gttataaaaa aaaaattctc atttttggcg ctatagtgaa
      121 gaggtgttac agataaacac atgtatctgc aagacattga ccaacaaaat atggagggaa
      181 gaaaaatgaa agatatggat atcaaggccg tctttatagg cgacaaagct gaaaatggac
      241 cagtttataa aatgttgttg aacaagatgg tagatgaaca tcttggatgg agagaaaatt
      301 atattccttc agatatgcct gcaattagcg aaggagataa gctcactcca gattatctag
      361 ccacccgcga ccatatgata gaggtcttgg atgaagtcag tcagcgatta cgcgcaggat
      421 cgatcccatg gcattcagca ggccgttact ggggacaaat gaatgctgaa acattgatgc
      481 ctgctttatt agcttacaac tatgcgatgc tgtggaaccc aaataatgtt gcattggaat
      541 cttctatggc aacttcgcaa atggaagcag aagtaggaca ggattttgct tcattattca
      601 atatgacaga cggatgggga cacattgcag cagatggttc tattgctaat cttgaaggat
      661 tatggtacgc ccgttgcatc aaatcgattc ctttagctgt caaggaagtg ctgcctgaaa
      721 aagtgaaaaa aatgtctgag tgggaacttt tgaatttatc tgttgaagaa attttggaaa
      781 tgaccgaaag ctttacggac gaagaaatgg atgaggtcaa agcagcttct tcccgtagcg
      841 gaaaaaatat ccaaagatta gggaaatggc tggtccctca aacaaaacac tattcttgga
      901 tgaaggcgtt agatatttgc ggagtcggct tagatcagat ggttgctatc ccagttcaag
      961 aggattatcg aatggatatt aatgctttgg aaaaaacaat tcgtgaatta gctggacaaa
     1021 aaatcccaat tctaggtgtg gttgctgtcg taggaacgac agaagaaggt caagtagaca
     1081 gtgtagataa aatcgtccaa ttaagagaaa ggttaaaaga tgaagggatc tatttctatt
     1141 tacatgttga tgccgcatat ggcggctatg cgcgttcact gtttctaaac gaagcaggag
     1201 aatttgtgcc gtatgcttcc ttagctgaat tctttgaaga acatcatgtt ttccaccact
     1261 gcgtaacgat tgacaaagag gtatatgaag ggttcagagc tatttcagaa gcggattctg
     1321 tcacgataga tcctcataag atgggctatg tcccttatgc tgctggagga atcgtaatca
     1381 aacataaaaa tatgagaaat atcatttctt atttcgcgcc atatgtgttt gaaaaatcag
     1441 taaaggctcc agatatgtta ggagcttata ttttggaagg atcaaaagct ggagcaaccg
     1501 ccgccgcagt ctggacagca catcgtgtat tgccattgaa tgtgaccgga tatggtcaat
     1561 tgattggggc ttcaatcgag gcagctcaaa gattcagaga atttctcgat catctgactt
     1621 ttactgttaa aggaaaaaca atcgaagtct atccattgaa tcatcctgat tttaatatgg
     1681 tcaattgggt attcaaagaa caaggctgta cagatttgaa cgctatcaat gaattaaatg
     1741 aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt ttatggtgaa cggtttatta
     1801 cttctcatac tacatttaca caagaagatt acggtgattc tccgattcgt tttgttgaaa
     1861 gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa aatcactctg ctacgtgcgg
     1921 ctatcatgac tccttatttg aatgatgatc gaattttcaa tttttataca aaaaaaatcg
     1981 ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata gaactgattt ttgtgtgaaa
     2041 gaataacaag agacatatgg agaacagaca attatttgct tcatcaaaac aattgtattt
     2101 ccccctgttt ttcatatatt cctcgccaag atattctttt ttataaaacg ttctataagt
     2161 tggatcgttc gtttcatgta tcaaacccag ttcactaaga tcgttcaatg gatgtttcgg
     2221 tgatactggg ttttgtactg aatcgatgaa accttgtcat ttctgttctt gaacaaaaat
     2281 aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa taaagatgaa agatactggt
     2341 tcatcaaagc aaatcacgac catgaccttt atcggaatga cctgcgccct tgtagcaagt
     2401 gtacgaaata ttccagatgt agcagcaacg agctggacga tgatttttta catgctggtt
     2461 gcagtattat tgtacgcttt tccaatctca ttgatctctg gtgaatttgc tggaatgttt
     2521 ccccaaaaag gtggtccaga actttgggta tcgaatgctc taggaaaaaa atgggggttc
     2581 gttgtctcat ggctgttatg ggtccaaatg tttcctggga tggtgatggt cgcttcagca
     2641 ctcgctccgt tgtttggtaa tattatcgat aatgtgccgc ttggtttgaa cagcaaattt
     2701 acattagttg tcattttagt cgtttattgg atcatcactt ttttgaattt gaaatttgat
     2761 atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat tgtacgtgcc tttgacgatg
     2821 atgctcttat tagggtttgc tgcgtggatc aaagttggga tcgtaccaac cggaacatta
     2881 ggcagttttt catgggacaa attaatacca gatactacga ctgcatcctc atttgtttat
     2941 tttgcgccga tcatgtttat ttttactggt atcgaaatgt cctctgtgta tatcactcga
     3001 ttggaaaatc cagttaaaac gtatattcgc ggagtatttg ccgcgttaat ttttatattt
     3061 ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg taccaaaagg acaaatggaa
     3121 ttgaataata tcgcgcaatc tatttcgatt tattgccaaa ttcttggttt gccacatctt
     3181 attgtgaatc tgtttagttt acttgtcttc attggtgtag cagtccaatt atctgcttgg
     3241 gcctcaggac cagcaaagac agtgacagaa agtgctagaa aaggtgcgta tccaccaaaa
     3301 ttcaattttt ggaagacgaa tcaatttgac gtatccaaat ccgttatttt gactcagtct
     3361 gtcattatct caatctttgc acttttttat ttgctgattc ctggcgtcaa tcaagcattt
     3421 ttgatgctcg taaattcaac tactgttatc tactgtatcg tctatgtcat catggggatc
     3481 gcagttttga gattgcgtta tacacatgca aagttgaatc gaccatttag aattggaaaa
     3541 aaagagaaaa agagcaatct tggagtttgg actgtcgtta ttgttttatt tgcggcaatc
     3601 ggattttctg tcggactgac gatgaaagct ggaacatgga tcaatctaat tgcagtcaca
     3661 gccatcagtg tcattttgtt tgtagtacca ctctgtattg aaaaaataaa gaaaccttct
     3721 tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag gtgaacttga tggaaaaacc
     3781 ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta atcgcattag gtggcgcaat
     3841 tggaacagga ttatttttag gttcagcaaa ggcaattgca tcagcagggc cagctgttat
     3901 tctcgtttat ttgatatgcg gtttggctat ttatggtttg atgcgggcca tgggtgattt
     3961 atttttagca aatagtacat ttaactctat cagtgatttc gttgactatt atttaggcaa
     4021 aaagtgggca ttttttgtca attggactta ttggctttgc tggcttggag ctggacttgc
     4081 ggaattaaca gcaataggat tgtatatcca tttctggttt gtacatgtac cagctttgat
     4141 aagcggaggt agcgcactaa tgatcttaat ggcagtcaac cttgttgcag tcaagtggtt
     4201 tggtgaattg gaatcttttt tctcaatcat caagatcttg gggattttcc tttttatctt
     4261 attgggtata ggattgagtg tctcagattt tcatacccag tctatcggaa tcaaagaaaa
     4321 ttttggacag atcgatgaat tactaccgtt tggctttgct ggaatggtag tctcatttcc
     4381 tatggtttta ttcagttttg ctggaattga aatgatcggc ttaaccgttg gcgagacaag
     4441 tgatccgcaa aagaatttga aaaaagcaat caactgtctg ccttggcgta ttctcttttt
     4501 ctatatagga acgattttgt cgattttatt tgttgttcca tggcattgtt tagatttaaa
     4561 agaaagtcct ttagtgacta tgctgcaagt gatccattgc caaggcggag cagcaattgt
     4621 caatctaatg attttggttg cttctttgtc ctcagcaaat agtgcagtct acagtactag
     4681 caggatttta tatcaaacag caaaagaaaa aagtttgccc cattggtttt gtgcgctttc
     4741 taagaaaggc gtacctttac acggtatttt aattacagga atgttatttt ttgccggtct
     4801 ttgtctacat ttttttgtag ctgatagtcg attgtttttt cagcttttgg caggcatgac
     4861 aacgagttgt tttctatttg tgtggtcttc gatcttgtgt gcacatgttc aatttattaa
     4921 agaaaagaaa cggaacacta cacaaagata taaagatctt gctttgttgg tgttttttac
     4981 ttcaattgct ttcagcttgt tattcgaaag cacgacacgc tttattcctt ttttattggt
     5041 tatctggttt gtattcttaa attttgtata taaaagaata agcaatagaa atatcagtta
     5101 ggcccagata cattttgcaa aacgaattat ttcagacccc cctgaaataa tcgatacgcc
     5161 tgaaaaaact tccctgcgta taataacagt atcaattaaa caggagggga taggatgaac
     5221 tggtacagat tgacggtaga agatgtattg aaaaactatc aaacatcaac agaaggcttg
     5281 tcgcaagaag aacgacagaa acgcctgact gatcaaggat ataatgaaat aaaagcaagg
     5341 cagcagacaa aacgatggaa aaaaatagca aagcatttta ctgatctgct gatgatcgtg
     5401 ttgatcattg cctctttttt gaaatttgct tctagtgaat atattgaagg aagtattatt
     5461 ttattcgtag tgatcgtcaa tggactcgtt agttactggc aagaaagaaa agcggaagaa
     5521 tcattgaatg gtttgaagca gctgatggga caagaagcga tagtactatc aaacgggatg
     5581 caagaaaaaa tccctgcgaa agaactggta aaaggagatg tcattgtctt acgtgccgga
     5641 gatgtcgttc cagcagatat ccgattaatc gaagcacatg atttattagt ggaagaatcg
     5701 attttgacgg gagaatcaga agcatctgaa aaaagtcatg ccgttttgac agaagaagaa
     5761 tcgatcggcg accagaaaaa tatgggtttt tcaggaacgc ttgtacaatc tggttctgca
     5821 gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg gtaaaatcaa tcaagcattg
     5881 caatcggtcg aacaacaaac gacgcctctg attaaaaaaa tcaaccgatt aaacaaacaa
     5941 atttttcaag gcattctttg tttgagccta ttcttagtta tttttacgac atttcgatat
     6001 ggtatggatt ggcatatcct tctttcggct acgattgctt tggtggtgtc aatggtaccc
     6061 gaaggattgc cagctgtttt gaccatgatt ctatccgttg gagtacatga aatggcaaaa
     6121 gaaaaggcga tcatcaaagg acttccttcg gtagaaacat taggttctat gacagtgatc
     6181 tgttcagata agacaggaac attgacaaaa aatgaaatga cagttgtgga tgtagctgca
     6241 aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc aggaattaaa aacaaaagaa
     6301 gaacagaaaa cagaaaattt aagtggcaat ccgacagaag ttgcgctgat tcgacacaca
     6361 gaaacagcca atctaccttt gaaaaaaata gaagcaaaga ttccgttcag ttctgaatac
     6421 aaatatatgg caacgatgca tgcggaggaa aaaggagcgg tgatttacgt caaaggtgca
     6481 ccagaagtat tatttgctaa atcaactctt tcttctgcag aacaagaagc atggagtcag
     6541 acagctgcag aatttgcgag aaaaggtcag cgtgttttag gatttgctta taaaaaagta
     6601 gattcgaaac aggaactcac gcatgagact ttaacccaat taacttttgc agggatcgcc
     6661 ggattgatcg atccgccaaa agaaagtgca gtcaaagctg taaaggaatg ccaacaagca
     6721 gggatctcag tgaaaatgat tacaggggat cacaaagaca cagcaaaagc gattgcggaa
     6781 caaattggct taaaacatac tgccaaagtt ttagaaggga tcgatttgga tcttatgtct
     6841 gatgaagagt tgattcagca agtgccaata gtcgatgtct ttgctcgaac gacaccagaa
     6901 cataaactgc gaattgttaa agctttgcag aaaaatggag aaatcgtcgg aatgaccgga
     6961 gacggcgtaa atgatgcacc tgctttgaaa agatccgatg taggaattgc tatgggaatc
     7021 aaaggtagtg aagtaagcaa acaggcagct gatatggttt taggagatga taatttccat
     7081 acgattgcaa aagccgtaaa agagggccgg cggattttgg ataatttgca aaaaaccatc
     7141 aatttcttct tgccaactgc attagctcaa ggattgatct tgatttgggc acttatgctg
     7201 aatcgcccgc tacctttgtc cccagtacag attttatggg tcaatatggt aacgacaatc
     7261 actttatctt atgcactagg gttcgagtcg ccagatccag aaatcatgaa gcgcccacca
     7321 agagatccaa agcaaggaat cctatctggt tatcatttgt ttcggatcat ctatgtctcc
     7381 ctactgatca tggttcctgc ctatttattg gctatgcagt ttgaagggca aagcttacag
     7441 caaaccattc ttctgcaaaa tatcgtctta gctcaagctg tttacatgat caattgcaga
     7501 gagttgtcca agccgtcatt gaaccaaggt cttttgcaaa acaaagcgct ttttctttca
     7561 ttaggtatcc ttgctttatt gcaagggatg gttatttttc taccagtcgg ccagcagctg
     7621 atcggtacag taagtttaac gatgacacaa cagttattga ttggagcaaa tgtgattctt
     7681 ctattcttag ttgtagaaat agaaaaaggg atcatgaaca aactgtttcg caaaaaagaa
     7741 aaaacagcta gagaatacag tcgctagccg ttactataaa aaagttttga aagtgagttc
     7801 agtcaaacga ttgagctcat ttttcgttgg aatagaagaa atgtatacaa tagggatatc
     7861 tagagaatct agagggaaat tggaaatcaa gatatctgtt ttttcaaaag aaatagtgga
     7921 aagatcggtt agttcgatag gtaagaagca gacacgtttt cctaagtaat cttttaattc
     7981 ctgctgcaag aacgccttcc atgaaggttc tccttggaaa ataaaataga cattgaacaa
     8041 attcggtttg atagaaaaaa tcgtttgttg gatcagtaga gacaagagtt ggagaaagta
     8101 atttggttca ttgatttcta tttctttttt tagatcgttc agtaaattct ctttctgttg
     8161 atacaagtgt gcatattgct gatcatatgt ttctaaaaaa cgatggtatt gacggtccaa
     8221 tgcttcaatc aatatcggcg attcatgata ttttaatatg aggagagcca agttatcagt
     8281 cagagatgtt ttttttagtt gatcaagaga cagctctgaa gctagttcct ctacaaacgt
     8341 ttcgaggacc tctaaaaatg gagaatgatg caggcagcga gcggtttctt gtccatagtt
     8401 attgttgtaa ttccaacttt ccagaaagca gaaagaagca aaagaaagct cagtgtcttt
     8461 aagataaata ccttccgtta aatacaattt tttcaatgca gagtcataca gggaggagag
     8521 tgtgctgttg gtatggatag aagaatctaa tcggcaattc gcttttatac ggatcgtatt
     8581 gataaagaac caacatgttc cgaaaatctc ttctgtttca accgctagtt cagtttgact
     8641 gaggtttctt aaaaattgaa aataatggga ttcatggatc gaataatcct cgaaaaaata
     8701 gttgttatga gtgaaaggaa tcagcaatcg atgataaaaa gtacgaatcg cacgctcttt
     8761 tccaagaata caagctgtat atttcgtcaa atcgatctgc aagtcgtaac ttgctaaacg
     8821 ctgattgatt ttagtcacat gtcttttcaa tgtcccataa gaaagcccat ggtcggctag
     8881 aaaatcaggg acagcaactt tttttgtaaa aagcagattt ttcagcagtt cgacactgac
     8941 tgattgtttg aaaaaaatct cccaaaggtc atttgttaat aaattttgct ggttttgcaa
     9001 actgattcct gcttgatcgc tgatgatgtc ccagccttcg ggcagctgag agcgaataga
     9061 ttgaaggtca gaaaaaatcg ttcgttcggt tgtccctatg atttcagcta attcatttac
     9121 ggttatcttt gtatgattca gcaattgttc tactaaagaa acatgacgtc gaatatcttt
     9181 ttcggtaatg atttttttca ttagtgaata catcaatcag actcccttct attctaagaa
     9241 tacggagaaa ataaacggtg gtcaagtttc aactgatttt tcaatcattg tttatttttc
     9301 tggagcattt actgatgata agaatttcat catgaacttg aaaaatgata ccactaaaat
     9361 caaaaaatag gtgggcaaac aataataaac attttaatag tagatggtag caagaccaac
     9421 acaggcggat tgaacattta agagttgata aaaaagaaaa atatcgaagt atttgattag
     9481 caactacttg tattcatttt tctattctta cgtggtatct ttgaataaag cgaatcaata
     9541 agaaagagat ggaagcagat gctaacgaaa aaagaactaa tcgatcacat caatgaaaca
     9601 tacaatttgt ctccagaata tatttttaaa aaatttcctg attattgcgt attcagacat
     9661 caaaaaagca aaaaatggtt tggggtcctg atgtctgttc cagaagaaaa aatctatgat
     9721 gatgcaagtg accataggat catcaatatt cttgatttga aggtcgatcc tgaacttggc
     9781 ggaattttgc gaggaaaaga ggggtatttt ccagcctatc acatgaataa agaacattgg
     9841 atctctattg atctacgtaa aatagaaaag ctagatgaac tagcagattt gattgatgga
     9901 agttttaaat caacgcaatg aatgtctagg acaatagaga agaggttgtg acagaaatgt
     9961 ttagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tggg
//
LOCUS       DASJON010000109         9811 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJON010000109 DASJON010000000
VERSION     DASJON010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.109"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..2154)
                     /locus_tag="U3989_001913"
     CDS             complement(34..2154)
                     /locus_tag="U3989_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1715359.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="U3989_001914"
     CDS             2384..3562
                     /locus_tag="U3989_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN1715360.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="U3989_001915"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="U3989_001915"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN1715361.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="U3989_001916"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="U3989_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN1715362.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="U3989_001917"
     CDS             complement(4915..6168)
                     /locus_tag="U3989_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1715363.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="U3989_001918"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="U3989_001918"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN1715364.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="U3989_001919"
     CDS             7746..9092
                     /locus_tag="U3989_001919"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN1715365.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="U3989_001920"
     CDS             complement(9400..9786)
                     /locus_tag="U3989_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1715366.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DASJON010000110         9732 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJON010000110 DASJON010000000
VERSION     DASJON010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.110"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..751)
                     /gene="msrA"
                     /locus_tag="U3989_001921"
     CDS             complement(<1..751)
                     /gene="msrA"
                     /locus_tag="U3989_001921"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1715367.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKI"
     gene            complement(926..2560)
                     /locus_tag="U3989_001922"
     CDS             complement(926..2560)
                     /locus_tag="U3989_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715368.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(2547..4415)
                     /locus_tag="U3989_001923"
     CDS             complement(2547..4415)
                     /locus_tag="U3989_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715369.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            4381..6018
                     /locus_tag="U3989_001924"
     CDS             4381..6018
                     /locus_tag="U3989_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715370.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(6163..6903)
                     /gene="truA"
                     /locus_tag="U3989_001925"
     CDS             complement(6163..6903)
                     /gene="truA"
                     /locus_tag="U3989_001925"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1715371.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(7094..7729)
                     /locus_tag="U3989_001926"
     CDS             complement(7094..7729)
                     /locus_tag="U3989_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1715372.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(7876..9117)
                     /gene="sstT"
                     /locus_tag="U3989_001927"
     CDS             complement(7876..9117)
                     /gene="sstT"
                     /locus_tag="U3989_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN1715373.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            9315..>9732
                     /locus_tag="U3989_001928"
     CDS             9315..>9732
                     /locus_tag="U3989_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715374.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVS"
BASE COUNT         3200 a         1781 c         1452 g         3299 t
ORIGIN      
        1 ggattttttt ataacgtttc ggttgttttt tgtaaaattg ctgatgatag ttttctgctg
       61 gccaaaacgt accggctggt tcgatttttg tgacgattgg ttggtggaaa gttcctgaac
      121 tgtctagttt ctgttttgat tgttccgcta attctttttg tcgttcgact tgataaaaaa
      181 tcactggacg atattgcttc cctctatctt gaaattgtcc aaatgtatct gtgggatcag
      241 taatttgcca atagattgtc agcaactcgc tataacttat ttctcttgtg tcataaatga
      301 tctccacagc ttccacatga cctgtgtaac caccacttac ttgatcataa tttggttttt
      361 caacatgtcc gcccgtgtat cctgacaaaa cagataggat cccttttttc gattcaaacg
      421 gctcgaccat acaccagaaa catccccctg caaatactgc acgttcttga tagtcagcat
      481 cttgcatggc atgcttggaa aagtcatatt tttgctcctt atcaaatcct tccgtaatca
      541 ataaataaaa gtccattaca tcaggcgtca gattcattcg gatagctaat ggacgtagtg
      601 ttgcttctag ttttgataac tgttctttaa cggatatatt ttctcttatg tcattctttg
      661 tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg attcaaaatc agattataaa
      721 gtgtttgaat gaatatttgt tcctcatcca tagattctcc tcctttccga agaagacaag
      781 ctagtgactt tagctgtaat ttatcataaa gtgaggaaat aagcgattac ttcatccccc
      841 tacagatttt tcgtataaaa aagagagaat ccttatcgac agattctcct ttttaatcaa
      901 tttattttat aaatgaaaag ttaatttacc ttttatggaa aactatttta aaaaataaac
      961 ataaatatgc agttaatgta acaagataag aaataatagt cgttactgta taacttgtgc
     1021 ttactttaat atcagcaact cctttgactc cgcttatttg taccaatcca ttagcattca
     1081 cctgctcctt tacaggttga tgatttattt ctacattcgt tcctaaataa gcatatacag
     1141 gaagtgtgat tacttggctc ttctcattag ttactctata tatgatagaa ctaccagttg
     1201 ttttataagg aaggtttacc cattgactgc caatctttgc ttcgtgtttt attatatgct
     1261 ccttattctc caatgaagcg ataggagaat aatcatataa aaaataatcc attcctttag
     1321 tggtatacgc ctctactgtt tctttgttta caacaatatg tcccggctga gaaattattt
     1381 tttgattgaa atttttttta ctagaaacag ttaaagtgat taataacaaa acagtcataa
     1441 ataaataaaa tagttctctt gtcttattgt atcttctatt tttgaaaaat atcatcgtta
     1501 atatcagaga agagaatatt atttgcaagc ttaagatccg ccaaggaaat tgtaacaatt
     1561 tggctggcgt attttgcaga agataccatg gcaccaaact agttgtagct agccaagtca
     1621 aacacgtacc ataataaatc tttttttcta tgtttgttaa ttttttaaat aaaatcaggg
     1681 gaatacataa agataacaga ccaactagtc ccattgagta agtagttaag tcgttgttca
     1741 tagcttcagt cagactatcg aacacattag atgcatgagc atataaattt gtcacgctag
     1801 gtttgttgat tttttggtag agcatttgtt caaacatcgg ataccaaaaa gcactcgtca
     1861 gaaccaatgt cattcctgcc gcactaaata attttttaaa tatagcaata aaatctgcat
     1921 tcttgaaacc tttagaaacc aaacgaataa aaataaaaat gataattaat gctgtactca
     1981 ttaatgttga tagtacatgg ctgtacacta gcaaactcat actcaatgct agctttacag
     2041 acggatactt ttcttctttt ccagagatga tttgataagc ataaaataat acagggataa
     2101 caagtgttat tgcgatagct tcggcaattg cggaacgata gtagatgtct actgtccgat
     2161 aagcagagaa aatatagaga caactaaata aaaatgccgc ttttttttgt tttaaaaacc
     2221 gctcgccata atgataagct acgagtgttg tacacaagtt caataaccaa acgtaaataa
     2281 tatagcttac aatcaaattt tttgtcatcc aatataacat tacagctgga aaaaaagtca
     2341 gaaagggata aaaataattt acaccatatc cagtataatt gaatgtctca taattaatcg
     2401 gactggtaaa aatagtcgat aaccccttga ttcgagataa gtgaaaagaa agatctccag
     2461 tggtataaat actctgtgta tttttgaaaa agatgaaaga atatatacta ctaactataa
     2521 ttaatccact caatattact acttttttat ttattttcat tcttcttgtt tatctccttt
     2581 ttagtcacat atcgagaatc tttcaactta ttaggtgcaa atcgtttaaa acttgaatac
     2641 accactaaac aataagtcag aatactgatc cataaagcca tgagagcagt tggagtatat
     2701 tttgttgtaa tttctatctt attttctcca gcatttacat ccacattcac taagccatgt
     2761 tttttataag agtcatttac gactttaccg tttactttta cctgtgtacc taaataagca
     2821 tatactggta ctgtcacttt ttgctttttg ttgcttgcca ctgtgtaagt aatgttatta
     2881 tctgcagctt tatacttccc tttttcccac gattctccta cttttacttc atgtttttct
     2941 aagtgaggcc tttcttttat cgcttctgtt ggggcgtaat cgtataatcc tcccatatct
     3001 gatgttgtat aacgtgggag tgtttcttca ttgataacaa tatgaccgtg ttttgattgg
     3061 ataacttttg cgtaattttc ttttgctgct atagacgtga cgagtagaaa caccacagta
     3121 gcgccaatga ttaaccatga atatttttta ttcatagtct ttttcataaa aacaagcgtt
     3181 atgatcattg aagaaaaaaa gatttggata cctaagatac gccaaggaaa ctggagtaag
     3241 cttatcggtg ttttttgaaa caaaaaccat ggaaataaat tagttacgca tacccatgta
     3301 gaaactacgc caacatagat gattttttct tttttctcca tttctttcag tatgaataaa
     3361 ggaaaaatca acgcaaccaa tcctaataac cccatagtgt atgttgtaat atcgttattt
     3421 aacgcttgaa tgatactatc tgaaatagaa agtgcttcta gctgcaaatc tgttttgaag
     3481 ggactgtgta tttcttgata attcatctgc tgcaacatag gataccaaaa atagctagtt
     3541 aatgcacaag tcaaaaaaac agcctttact aaattcacga ccacaaattt tatatcgttt
     3601 tttatttgct tgcttaaaaa caaacgaact ataatcatac aagcaatcaa tgcactacac
     3661 aatagtgtag ttaagacatg agtatacaca agtagactta tactgacaga taaagcgatg
     3721 actttttttt ctctattgaa tatcaatcca taagcataat ataaaacagc tgggataatc
     3781 gtaagagcga ttgcttctgc aattgcggat cgataataga tatcaactgt tcgataagtt
     3841 gcaaaagtat ataaacaact aaataaaaaa gcagcatcta tctttttaaa aaacttttct
     3901 ccataataaa aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa
     3961 cttaatatta agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa
     4021 ggataaaaat agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct
     4081 tcaaaaatag aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta
     4141 taaatttctt gagcatttct cataaaattt actgaatata aagtactaag taagataaga
     4201 actactaaaa gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat
     4261 tctattttca gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata
     4321 gcactagtat gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa
     4381 atgaaaaaaa acaaaaacca aataatagat atcattttta tgttatttct aagttttgct
     4441 tacgtcatcc ctctattcat gtctaaggga atctatcata gtgtcaatca ggatacttac
     4501 tttcatttat ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat
     4561 aacttcgatc atcatggaac aatgatgaat attttttatc catggcttac tctttatcca
     4621 gcttttttat tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt
     4681 tttatcactt ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat
     4741 cgttatattt cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac
     4801 atattccatc gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta
     4861 atggggtgtt acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc
     4921 atgacgctag tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga
     4981 ggaacgttgt ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt
     5041 ttaaaagcta ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa
     5101 caatctagag cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct
     5161 agtgatatgc tgacacatat tttaaataat aactataaca actatactat tggattattc
     5221 ttatttctcg gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta
     5281 tttattttct ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa
     5341 ctttttaacc atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct
     5401 tctttattta tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat
     5461 aattcatgga aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta
     5521 agcaattcta tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt
     5581 gctatagctg aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct
     5641 gatatgatta ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat
     5701 tttatgagta acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg
     5761 acgacaccta tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa
     5821 acaaaattat ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc
     5881 gttattactt atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact
     5941 ttgattttat ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa
     6001 gtacaacatt ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag
     6061 gctaaaaagt ctttttttat atttctttaa tttgcttcat acattattta acatcgatat
     6121 ttatactgtg aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc
     6181 atccaaaaac agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc
     6241 aaatatttca tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc
     6301 catgataatc cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat
     6361 ttctcccttt cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt
     6421 tttcaatgca gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt
     6481 cagcttgtcc atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga
     6541 aggaatcacg ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga
     6601 atggaagcgc tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg
     6661 attcataaag tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac
     6721 ttgtcctcgc gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc
     6781 atttgtcatc tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc
     6841 aagtctttgc catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct
     6901 catattttag tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta
     6961 gcacaaaata tagctccttg cttctctaag acagaagcta tacaagaaaa ctgtggaaat
     7021 atatatttca gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt
     7081 gacggctcaa gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat
     7141 taaagaaaaa actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta
     7201 tagaaggaat tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc
     7261 caaggtataa gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa
     7321 ctggaaacaa taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg
     7381 agacattcca cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac
     7441 ctataccata gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactaa
     7501 caaatgcagc atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc
     7561 aaataagaaa cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct
     7621 tcatttgatc taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca
     7681 gcacgcctaa taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc
     7741 aataacaatt acctatgctt ttaattgtta ttataaacga ctttatcttg atttaacaga
     7801 aacctctatt tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata
     7861 ctaaatatct aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt
     7921 ttccccactt cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga
     7981 cgaatttaag gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac
     8041 gatttgcatc gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa
     8101 cagtgaaccg cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa
     8161 aaggactttc aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat
     8221 caaggaaatg gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat
     8281 ggaataagac tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt
     8341 tgcggcagaa cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat
     8401 caatggatag ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc
     8461 gacaaacagc atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc
     8521 gattccagtc gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc
     8581 aataatcact tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt
     8641 cgattctgaa cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag
     8701 gtaattcgca tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga
     8761 taaaacactt tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg
     8821 aacagtaaaa attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa
     8881 atataagatc aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga
     8941 cgccatcgtc aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa
     9001 cgttcccaat attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac
     9061 taaaatacca acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg
     9121 accacccttt cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat
     9181 aattttctaa aaattctaac aatacaaaca accatttctt gctaattttg tactttttct
     9241 gtctacacgt tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca
     9301 ggaaacgttt tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa
     9361 taaagaagga gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac
     9421 agcgtatatt gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct
     9481 acatctatac atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca
     9541 tgggttacca atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg
     9601 cctttatatt cctaatagcc acactagttt gcaatatctt tttttacgta caagctggca
     9661 gcttgaatat tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt
     9721 atgctgtttc tt
//
LOCUS       DASJON010000111         9554 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJON010000111 DASJON010000000
VERSION     DASJON010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.111"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(16..678)
                     /locus_tag="U3989_001929"
     CDS             complement(16..678)
                     /locus_tag="U3989_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715375.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(691..2193)
                     /locus_tag="U3989_001930"
     CDS             complement(691..2193)
                     /locus_tag="U3989_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1715376.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2741..4420
                     /locus_tag="U3989_001931"
     CDS             2741..4420
                     /locus_tag="U3989_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1715377.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4547..5026
                     /locus_tag="U3989_001932"
     CDS             4547..5026
                     /locus_tag="U3989_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN1715378.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5245..5925
                     /locus_tag="U3989_001933"
     CDS             5245..5925
                     /locus_tag="U3989_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN1715379.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5933..7264
                     /locus_tag="U3989_001934"
     CDS             5933..7264
                     /locus_tag="U3989_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1715380.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7279..8241
                     /locus_tag="U3989_001935"
     CDS             7279..8241
                     /locus_tag="U3989_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN1715381.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8352..8690
                     /locus_tag="U3989_001936"
     CDS             8352..8690
                     /locus_tag="U3989_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN1715382.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8769..9476
                     /locus_tag="U3989_001937"
     CDS             8769..9476
                     /locus_tag="U3989_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN1715383.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3035 a         1722 c         2051 g         2746 t
ORIGIN      
        1 tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac
       61 ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg
      121 ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt
      181 tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc
      241 gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc
      301 aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt
      361 agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta
      421 agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag
      481 tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga
      541 aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc
      601 gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa
      661 ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa
      721 tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc
      781 ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga
      841 aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg
      901 tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt
      961 ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc
     1021 aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt
     1081 acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt
     1141 aatcataatg atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt
     1201 tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt
     1261 tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc
     1321 cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt
     1381 cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa
     1441 gttgaatttc atacccatac tgccgcttgt ttctctggat tcataaattc ctttgatcgt
     1501 catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc
     1561 caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct
     1621 gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg
     1681 tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc
     1741 cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc
     1801 agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga
     1861 ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt
     1921 aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg
     1981 ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc
     2041 tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac
     2101 aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt
     2161 tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat
     2221 ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca
     2281 acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg
     2341 aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct
     2401 agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat
     2461 ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag tattctggct
     2521 atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc
     2581 caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa
     2641 tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg
     2701 acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat
     2761 cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga
     2821 tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt
     2881 cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt
     2941 aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt
     3001 accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga
     3061 agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg
     3121 tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa
     3181 tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc
     3241 tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt
     3301 agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca
     3361 aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg
     3421 tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg
     3481 aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga
     3541 aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga
     3601 tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca
     3661 aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat
     3721 cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta
     3781 tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa
     3841 tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca
     3901 aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata
     3961 caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag
     4021 tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat
     4081 cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt
     4141 tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg
     4201 attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac
     4261 aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat
     4321 ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc
     4381 agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac
     4441 ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca
     4501 gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac
     4561 aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca
     4621 tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat
     4681 atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt
     4741 gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt
     4801 tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat
     4861 tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa
     4921 agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga
     4981 tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga
     5041 agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc
     5101 tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt
     5161 tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata
     5221 cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca
     5281 attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg
     5341 acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt
     5401 gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt
     5461 gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt
     5521 ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc
     5581 tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca
     5641 gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt
     5701 gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg
     5761 ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat
     5821 tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat
     5881 agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac
     5941 aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt
     6001 aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga
     6061 tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg
     6121 caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg
     6181 ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc
     6241 gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa
     6301 ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg
     6361 attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg
     6421 cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt
     6481 gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta
     6541 tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc
     6601 gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt
     6661 tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt
     6721 aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt
     6781 catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat
     6841 gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat
     6901 atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa
     6961 gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt
     7021 acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa
     7081 tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt
     7141 agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt
     7201 gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc
     7261 ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac
     7321 gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta
     7381 ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa
     7441 acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa
     7501 ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat
     7561 cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat
     7621 cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg
     7681 atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc
     7741 gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct
     7801 tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg
     7861 ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat
     7921 ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca
     7981 gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt
     8041 tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc
     8101 aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca
     8161 aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat
     8221 aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac
     8281 aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa
     8341 ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag
     8401 atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa
     8461 ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg
     8521 atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag
     8581 gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag
     8641 aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca
     8701 aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg
     8761 aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca
     8821 ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat
     8881 acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac
     8941 aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact
     9001 acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg
     9061 ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg
     9121 tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt
     9181 tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga
     9241 ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg
     9301 cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac
     9361 cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga
     9421 cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata
     9481 aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata
     9541 ccatttattt ggaa
//
LOCUS       DASJON010000112         9459 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJON010000112 DASJON010000000
VERSION     DASJON010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.113"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..688
                     /locus_tag="U3989_001938"
     CDS             <1..688
                     /locus_tag="U3989_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN1715384.1"
                     /translation="ENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKKADPYSDMMK
                     LNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQEL
                     KAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETE
                     KDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQL
                     QENTEITIQF"
     gene            752..4384
                     /gene="pglX"
                     /locus_tag="U3989_001939"
     CDS             752..4384
                     /gene="pglX"
                     /locus_tag="U3989_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HEN1715385.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            4500..7022
                     /gene="pglZ"
                     /locus_tag="U3989_001940"
     CDS             4500..7022
                     /gene="pglZ"
                     /locus_tag="U3989_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HEN1715386.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            7140..9173
                     /gene="brxL"
                     /locus_tag="U3989_001941"
     CDS             7140..9173
                     /gene="brxL"
                     /locus_tag="U3989_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HEN1715387.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         3436 a         1271 c         1845 g         2907 t
ORIGIN      
        1 ggagaatgca caagataaaa ttagattaat cgaaaaaagt cggcgtcaaa cttctagcca
       61 agaaattgat caattgattc atgagatgaa aaccattttg aaaaaggctg atccttattc
      121 agatatgatg aaactaaatg agttgattca agaatttatt gaacaatata atcaactgtt
      181 agagttggaa agtcaggaaa tattaaataa attaacggaa gaaaaacaag caacatttaa
      241 attattagaa ggaaaagatt ttaaggatca atttgtacca atagttgacc aagaatttca
      301 agaactgaaa gcaaaaattg aacaagcgaa tactatagat gatcttgttt tcttatcatt
      361 aaacgtagca gaacagtatt tgatctttaa tcaacgtttc gataatgaag aaaaccgcag
      421 acgtcaagaa gaacaagcac gtaaacaaat tgaagcagca aacttgattc gtgaaactga
      481 aaaagatgaa atagactctg cagcaaaagc tgaagaaata gcgatagtaa aacctactat
      541 tcaacagaaa acagtaccaa tgagaacact taacgttcat gttaaaacta ttaagtcagc
      601 agcagatatt gatgagttaa cagctgaaat taatcaaaaa ctgaaggaac aacttcaaga
      661 aaatacagaa atcacgattc aattctagtg aaaagacagg cataaaaagg tgcctgtctt
      721 ttcaaaggtt acatatagaa gggaaagaaa aatggataag aaagcgataa agaatttcgc
      781 ggtcaatgcc cgtagaaaat taattgatga agtaatgact aaagcaaaac gtttaggaat
      841 tgatgaatca aaaattgaag atgtgaagaa aattgatagc aatttacaag aaatcgaaaa
      901 atcaggaata agaattcaag gaaaagaaat agcgcaaaga aacaaattga ttgcagaact
      961 aaaacaacgt gcttctgtca cttcttattc agaagctttt caagagttaa tcgaagaggt
     1021 tgcttatacg tggtttaacc gattgattgc cattcggttt atggaggtaa atgattattt
     1081 acctgacacg tatcgtgtat tatctagtga aataagtggg aaaattgagc cagatattat
     1141 tacagatatt tatgatgccg atatctttga agaattatct agtaatgaac agcaacaagt
     1201 cagaacttgg aaaacagata attcagcaga agcaatggac aagctgtatc aattgatttt
     1261 tataaaaatc tgtaatcaat tgaatgatta cttacctgag ctatttgaag aagtagaaga
     1321 ttatacagaa cttttgttta cagcttctta tattaaaaaa gatggtgtaa tcgctgatct
     1381 tttaagtatt ccagaggatg atttctatgt tcaagctggt ggacaggtag agattattgg
     1441 gtggttgtat cagtactata atagtgagcc acataatcaa gtggtgaata tttataaagg
     1501 gattgtcaaa aaacaagata ttccggcagc aactcaacta tttaccacag attgggtcgt
     1561 tcgttatatg gttgacaata gtttaggaaa gtattggtta gatcattatc ccaatagtga
     1621 attaagaaat aagttgaaat acttaattcc agataaatat gcttttgaac aagcaacatc
     1681 tgtaagtaat ttaaaaccag aagaactaaa aattatcgat aacgcaatgg ggtctgggca
     1741 tattttagtt tacgccttcg atgtattgat ggaaatttat cttacgttag gatattctga
     1801 gcgtgatgct gcacgtgcaa tcgttgaata caatttatat ggtcttgaaa tcgataaaag
     1861 agcgtatcaa ttgtcttatt ttgcaattat gatgaaaggg cgtcaatacg atcgtctttt
     1921 tttgaaaaaa agtctatcgc cgaatttaag agagtttatt gattctccta aagtgacaga
     1981 cgagtatcaa gagcgtatta gtgaattgaa caatgataag tatagtgaac gctggaatga
     2041 atacaaagag ttaatttttg atcaatttga aaatgggaaa gaacttggat caatcattaa
     2101 gttagtcgat aaaaataatc aggcaataac taaagaatac ttaggaaaag taagagagta
     2161 tatatcaaaa ttcaacgaat ttacgaatat ggatattctt tatgggatga aggaaataca
     2221 agaaaaatac ttgcacattc ttgacgtggc tgaaatgcta gttgataaat atgtagcggt
     2281 agtcactaat ccgccttatc ttaataaaat ggataagaaa ttaaaaaaat atgtaaatga
     2341 taactattct gatgtcaaaa aagatctatt ttctgtgttt attaaattga atagccaaat
     2401 gctgattgat aatggatatg cttcctttat gacgccattt gtttggatgt tcatttctag
     2461 ctatgaaaag ttacgctcat ttttaattaa tgaaagaaac atttctagtt tgattcaaat
     2521 ggagtattca gcttttgaag aagcaaccgt tccaatttgt acgtttacga taaaaaattc
     2581 aaatgaacaa gatggaagct atataaaatt atcagatttt aaaggtggaa tggaagttca
     2641 aaaaattaaa acgttagaag caattcatca gcatggtgaa tgcgattatt tttatagtac
     2701 taatcaagac aacttcaaaa aaatcccagg aatgccgatt gcttactggg caagcgataa
     2761 cgtaatcact gattttgctc aaggaaaatt aactaacgat gttgcagatg caaaacaagg
     2821 gttggcaacc ggaaataaca ataaattttt gcggtattgg tttgaagtag aatttaacaa
     2881 aataaagttt gatgctaaaa cattagagga cgctaaaaag tctagaaaaa aatggtttcc
     2941 tacaactaaa ggtggtcgtt ttagaaaatg gtatggaaac aatgaatacg tggttaactg
     3001 ggagaatgat ggagatgaaa taaaaaatta tgttaatgaa aatgggaagt taaaatctag
     3061 accacaaaat cttaattact attttaaaaa gggaattact tggtcgacac tttcatctgg
     3121 acaagtatct tttagattgc agaatcaggc aatttttgaa actaaaggat ctgttctgtt
     3181 ctcaaaggat ataactgact ttgaagaaat atttggctat gtaaactcta aattagttaa
     3241 tcacttttta acttttattt ctcccacctt ggattaccat gaaggaccaa tgggaaaact
     3301 accttttctt aaagttacat ctgagagaaa attaaaaaaa ataagagaaa aagtggatga
     3361 aaatatcgat atagctagaa aagactataa tgctcttgaa acgtcatacg agtttagaag
     3421 aaacattttg ttaagttata tgtttaaaaa agatctctca gaggcattta actgtctaag
     3481 taggcagttg gttaatcaat ttaatactct aaaatcgaat gaagaggaac tgaacgacat
     3541 attcattaat cattattctc ttgaaaaaga attaacaaaa gaggttcaag atgaatatat
     3601 ctctgcaagg ttacctgata aggcgaggga aattaaggat tttatttctt attcaatagg
     3661 ttgtatcttc ggtcgttact ccctagacga agatggtctc atcttcgctg gcggcgaatg
     3721 ggatccttcc cgctatcaaa aatttaaacc tgatactgat aatgttttac tcatcaccga
     3781 tgatgagtat ttcaaggata gccaaattga cattgtcaat cgtttcattg atttcgttga
     3841 gattactttt ggtaaagaaa cccttgaaga aaatctccag tttatcgctg acactttagg
     3901 tggcaaaggg acaagtcgag aaattattcg tcgttacttc gtacaagatt tttacaaaga
     3961 ccatttaaaa acatactcca atcgaccaat ttattggcaa ttggactcag gaaaacaaaa
     4021 tggctttaaa gcgttaatgt acttgcatcg ctatacacca gatcaattag gaaaagtacg
     4081 tactgaatac ctacacgaac tacaaaaagc gtatgacaat cgaatcgaat tgcgtcagca
     4141 acaaatttca acaagtgata acccaaaaga aagaaatgca atgacaaaag aaattgtaaa
     4201 gattcagaag caattaaaag aagttcgtga gtatgacgag aagttgggtc acttagcatt
     4261 aaaacgaatt gaattagact tagatgatgg tgtaattgtc aattatgata agttgcagcg
     4321 tgatcctaaa accggcgaga aattccaaat ttttgcaaaa ggggtcaaag aaccaaaaaa
     4381 atagttatct aagaaagttg ttttctaaca actttctttt ttattcttaa attgcgtgcg
     4441 aaattatcca aattatgtca atataagggg tatagaaaga gaacgattag gtgagattga
     4501 tgacagatat aaatacgcaa caaattgaag aaaaattaaa tcaacgtttt cctaattatg
     4561 gatgtaggaa aatcattttt tggtttgatt caaatcagga ttttcttgaa gaaattgact
     4621 cattggtcat tcaaaatgca aaaatctatc gattagaaaa ggacgcacaa tttaaaacta
     4681 agcgtttatt ggaatatgaa gatcccgaaa ctaactattt aatctatgcg ccatttcctc
     4741 gttgggatga taaagatgaa gataatcatt tgttgagtgt attgaattat tcagaggaat
     4801 ttagtgcaga ccgcttagcg attatcatga atgaattggc tattccactt agatttcatc
     4861 agacagttaa acagtatcgt aatttcttta atgcaaagga tcgcatgaga agttttggtg
     4921 aatttagtac gcatatgaca attaataaat cagaaacgat tgaattaatg ataatggctg
     4981 ttttgttgaa atcgaaaagt attcgattaa atgatttact gcgtgtagtt ctaaaaagct
     5041 ttacagaaag tagggagaat cctttaaggg aacttgataa gtatcagtta acagctaaat
     5101 tttgggaatt tattcaaatg aaatttggct ttacggatag ccagccaaca ttggaaaagc
     5161 tgacgatgtg tctttatttg aactattttt attatcaaat tgatcaagag atgccgaaga
     5221 agttcaaagg atttgaaata ctacatttga aagcaaacat tattactttc atggatcagt
     5281 ttatgaatga tacacgttac atggaaagct ttgaacagct atctaaaaaa gtatatcagg
     5341 ctattgatgg tgatggatta ttagacagta tagaaattga tgatctaatt gaagcagacg
     5401 tattttctga gattcatgca aaaatattaa tgttttatgc agaacgatta aaggcttctg
     5461 acactacaac gaaaattgca ggacaaacaa tttttgatag tttaaagcag cgcgaaaaaa
     5521 tgcatttcgc atcggattat atttatgagt atcaattatt gaaacatgct tattatcttg
     5581 tggataaaaa cagattgtgc ttgccgatga acatggataa tttgctaact caatatgaga
     5641 aggaaatgta ttgggttgat acgcattatc ggaaatttat ttggaatagt gatcatatag
     5701 aagaaaagca aaaatatggc caattgtatc gattagttga agcaaattat tcgaagtttc
     5761 ttgatcaatc aggacaaatt tggaatcaaa cttttgaatt taagaatcgt ccatccattc
     5821 gaaaatttta taatcaagct gtaatgaaac gctctgttaa aactgtagtt attatttcag
     5881 atgcttttcg atatgagtta gggatgaatt tagaacgtaa gttagttaga gaaaagaagt
     5941 attcaacaaa catggctagc tttctttctg tattaccttc agttactgaa ttcggtaaag
     6001 ccgccttatt accaaacaat caactaactt acaatgaagg aacagaagta ctgattgatg
     6061 ggaaaaaaac gaatggttta tccaatcgaa atgcgattct ccagcaacga gatgcaaatg
     6121 ctgtagcttt aaattatgat gatgtggtta agatgaataa ggctgaggta cgagaagtat
     6181 ttaatggcaa atcagtcatt tatatttatc acgatcaagt ggatagaaca ggtgatcact
     6241 cgaatgaaaa tcgagttttt gaggcctgtg atttagcgat aagcgaaatt tcccgactta
     6301 ttaatcgctt gcataatgat ggcagtatct atcgttttat agttacggct gatcatggat
     6361 ttatttataa aagaagccac gttgaagaag tagataagat tgaaaatcca tctagaaatg
     6421 aaaaagatcg tgtagaacgt cgttttattc ttagtgaaga agaatacgaa ataattggta
     6481 cgaaaaatta ctctttggga gattctttag gaaatgatga cactagaaac atttgtgtcc
     6541 cgattacttc aagtattttt aagaaagctg gcgggggaca aagctacgtt catggagggt
     6601 catctattca agaaatgcta gtccctgttc tagaaattac ggtagccaaa ggtagcaatg
     6661 caaaggaacc agtagcaatt gaattaatga ctagcaaccg cagaattaca ggattatcaa
     6721 ccacgttaga attctatcaa aaaaatgcaa ttagtgatgt ttatgacccc gcatctttct
     6781 ggctttattt tgaagatgaa tatggtgaac gtatctcaaa tgaagaaatc tatgttgcgg
     6841 attcaaaaac agaagatgca aatcagcggt ttactaaatt tgtttttgaa tttgtaaatc
     6901 gtcgatatac agttgaagat acttattatt tagtgataaa aaatcaaaaa aatgatttga
     6961 ccgagcgaat aaaattcatt atagataatc cttttgcaga atcatttgat tttgatatat
     7021 aaaaacgagg tatccacaat tttttgtgga tactttttct tttgtattat caagcctact
     7081 ggttaagtat gatatacttt aattgctatt ataatgactg aacacatgga ggaattctag
     7141 tggaacaaca ggaaatttta aagttatctg aattctttcc cggaaagatt gtcagaaaag
     7201 atttaacaaa gaaaattaaa gaaggtgcga atgtacctgt ttatgtattg gaatatttac
     7261 ttggtatgta tgcttcgtca ttggatgaaa cattggttga aaaaggtgtg gaaaatgtta
     7321 aaaatattct tgctcgaaac tatgtgcgtc ctgatgaagc agaaaaagta aaagcaaaaa
     7381 ttcgtgaatt gggtggttat acagttattg ataaagtaac tgtacattta aatgaaaaga
     7441 aagattgtta tgaagcagaa ttctctaacc tgggattaaa aaatgttcaa attagtgaag
     7501 agtatccgaa aaaatttgat cgattattag gtggggggat ctggtgtatt gtttcgatgg
     7561 aatattggta tgatgagaat gatcgttgga atagcccttt tattgcaaaa aaggttgatc
     7621 caatccaaat gcctggttta gacattgagg cttacaagat ggctcgcaag gagtttacta
     7681 caaatgaatg gattgactta cttctacggt ccattggtat ggaacctaca caatttaata
     7741 aaagggtaaa atggcttcat ttagcaagac ttattccatt aattgaaaat aattttaatc
     7801 tctgtgaact aggaccacgt ggtacaggta aatcgcatgt atataaagaa atttcaccaa
     7861 actcaatcct tgtttcaggt ggacaaacaa cagtagcaaa ccttttctat aatatggcta
     7921 gtcgtacaat gggacttgta ggtatgtggg actgtgttgc ttttgacgaa gttgctggga
     7981 ttaaatttaa ggataatgat ggcgttcaaa tcatgaaaga ttacatggct tcaggatctt
     8041 ttgctagagg caaagaagaa aagaatgctt cagcatcaat ggcttttgta ggaaatatta
     8101 atcaaagcgt tgatgtaata ttgaaaactt cgaatttatt tgagccattt ccggaggtta
     8161 tggggacgga ttcagccttt ttggatcgga tgcattgtta tttaccaggt tgggaaattc
     8221 ctaaatatcg accagatttt tttacaaatg actatggctt cattacagat tatttttctg
     8281 aagtcatgcg tgagcttcga aaagttagtt ttggcgatag ttttgataaa tatttcaaac
     8341 ttggttctca attaaatcag cgggatacga ttgcagttcg tcggattgta tctgggatga
     8401 tgaaactgtt gtatcctgat ggtagatttg aaaaaagtga tgtcgaagaa atactaagat
     8461 ttgctcttga aatgcgtcgt cgtgtaaaag aacaattaaa gaaaattggt ggaatggaat
     8521 tttatgatgt caatttttca tacattgatt tagaagaaga gcgagaagag tatgtttctg
     8581 ttcctgaaca aggtggagga actttgattc cagaagggtt agggaagcca gggcatttgt
     8641 acacggtatc ttatggcaaa actgggcaat taggtaccta taaattggaa acgcaaatga
     8701 cttcgggaaa tggaaagttt gaacgtactg ggatcaattc agatgctgga gcaagagaag
     8761 cgatggattc ggcataccga tatttaagag ctaattatgg aagtgttagt cagtctatta
     8821 gtttgacgac aaaagatttt gtcgttcatg ttcaagatat gaatggcatt ggtatgacaa
     8881 aaacattatc gttatcaact ataatagctt tatgctcagt ttccttaaat aaacctgtat
     8941 taccaagttt agttatttta ggggatatta caattggtgg ttctatgatt aaggtagata
     9001 atttagctaa ctgtttacaa gtagcaaaag atgccggagc gaaaaaagta cttgtaccta
     9061 tgataagcgc tgctgatatt ggaactgtac ccccagaatt aatgggtacc tttaatttga
     9121 ttttttatca aacgccggaa gatgctgtct ttaaagccct tggggtggaa tagacaactt
     9181 ttccatttgc tggtttgact acgtaccaat ctatattact tactagatta gtcgttaatc
     9241 tattcggatc gaatgggcaa aatatttttg tgtatgtccg tccttgatca tgattccaaa
     9301 aagaatcatt gggggctgtg gcgtaaatat tatactataa gccagaaata cctatattta
     9361 gttatttctg gattttttga tataaaaata acagaagaat aatacaaata gcctaatact
     9421 agaatagtta gtcatttttt tacctatcat tctgaactt
//
LOCUS       DASJON010000113         9213 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.114, whole genome shotgun sequence.
ACCESSION   DASJON010000113 DASJON010000000
VERSION     DASJON010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.114"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..471
                     /locus_tag="U3989_001942"
     CDS             <1..471
                     /locus_tag="U3989_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1715388.1"
                     /translation="SQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIV
                     DRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFL
                     PQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            591..791
                     /locus_tag="U3989_001943"
     CDS             591..791
                     /locus_tag="U3989_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1715389.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            867..1064
                     /locus_tag="U3989_001944"
     CDS             867..1064
                     /locus_tag="U3989_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715390.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1361..2908)
                     /locus_tag="U3989_001945"
     CDS             complement(1361..2908)
                     /locus_tag="U3989_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEN1715391.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(2992..3795)
                     /locus_tag="U3989_001946"
     CDS             complement(2992..3795)
                     /locus_tag="U3989_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1715392.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(3798..4580)
                     /gene="phnE"
                     /locus_tag="U3989_001947"
     CDS             complement(3798..4580)
                     /gene="phnE"
                     /locus_tag="U3989_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN1715393.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4577..5356)
                     /gene="phnE"
                     /locus_tag="U3989_001948"
     CDS             complement(4577..5356)
                     /gene="phnE"
                     /locus_tag="U3989_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN1715394.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5328..6119)
                     /gene="phnC"
                     /locus_tag="U3989_001949"
     CDS             complement(5328..6119)
                     /gene="phnC"
                     /locus_tag="U3989_001949"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEN1715395.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6180..7109)
                     /locus_tag="U3989_001950"
     CDS             complement(6180..7109)
                     /locus_tag="U3989_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1715396.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7304..7987
                     /locus_tag="U3989_001951"
     CDS             7304..7987
                     /locus_tag="U3989_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN1715397.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8148..9191
                     /locus_tag="U3989_001952"
     CDS             8148..9191
                     /locus_tag="U3989_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1715398.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2838 a         1883 c         1825 g         2667 t
ORIGIN      
        1 tctcaacgag ggttagggaa aaaattgaaa cgctattgga aactcctaca aaaagaagaa
       61 gcaaagttaa actacgaaaa aagggtttgg cgtgccagtt ttaaagatta tttgactgaa
      121 agtgagattg tcgatcgact gttatctgct tgtcccaacc tgcgccaagg ctatcagtta
      181 taccaagaca ttctttatgc ggtaaagaaa agagatcagt cattatttga ggactgttta
      241 aacagagaag tcagcggtct tcctgaaacc tatacaacca ctttacgaac gtttaaaaag
      301 tttctgccac agatccaaaa tgcgcttcac tacagctatt caaatggacc attggaatgt
      361 ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg gctttcgcag cttttacaat
      421 ttcaaactac ggatcatgat tcgtcatggc aaaacatttt taaccaaata aacagaacag
      481 aaagagagtt tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat
      541 catttgacaa agagcctgta tttattactg tcgaggacaa tggcatcggc atgattgaag
      601 aggaacaaaa aaacatttgg gatcgctatt ataaagccga tccgtcaagt aaaaatacaa
      661 agataggcga gtcaggtctt ggattatcaa tcgtgaagga actagtgcgc ctgcaccacg
      721 gttcaattga agtgaccagc cgaaaaaatg aaggaacgac cttcctgata caatttccaa
      781 aagaaaaata aaattaccag taaaaaaccg tggagagata gctgctattg atttttgcta
      841 tgtcttttct ttgagaggta tccggtatgg tggagtgtct gaaaaataaa aaaatggagg
      901 ccaattttgt gaaagctgtt atttctggaa gtattgtttt ccaagaagaa tatcgagata
      961 tggaaaaaga tttcagtaaa aaaggcattg aattaattga ttatcctcgc tcatgtccga
     1021 tcctagacaa agagtatcca gaaatcttga cgactttttt ttaaatatcg cagcagcgga
     1081 tttatttttt gtatttaatc aagatcggaa aggggtatta ggctatattg gtgccgcaac
     1141 tttttcagaa atgaagtatt gtctgatgca aaatcttatt catggaaaaa ggatcaaaat
     1201 agtcttattt aaggaaccat cagaggaaaa ttttttgttt tgatgaggta gatcgctggc
     1261 taaagaatgg ctggatggaa atcagacagc ctaacaattt ggcataacaa aggtgtgggc
     1321 aaaaaactgc tcacaccttt ttgaattttt tatttatata ctacaaaatc acttgaaaat
     1381 tatgattcac ttttgcttcg atgcgtgggt gaatattcaa atagtcggca attaattcgg
     1441 tcatgtcgat ttggatttct cggacaattt tctccgcacc aaacatcgca taatttcctc
     1501 cgccgattgc ccgatattga tttgtcacca cttccagcat ttgttgcgga agaacggggt
     1561 gacctttaaa acgaagttcg gtaatccgtt gtccaacagg ctgtttcata tcaatagtgt
     1621 actgaatccc ttcatacatg tcataattgt agtactgggg cttaggctga ataaaggctg
     1681 gattgaatac gatttggtta ttttctacag ctaaataatt tgcggtctgc tccaacgccg
     1741 ctctcaaatc tgcaccattt atttgaatca ctgccagagt attcggataa atatagttgg
     1801 tgatgacgtc ccgcatcgtg atcgtctcac caaagccttt tccttcatta ttgaataacg
     1861 cagttccaga aatctctgag ccgctggcaa ataattgtac acgattaata aactctacat
     1921 agggatgttc ctcaagacga gcctgcatcg gatcattaat acgcatattt ccgttcactt
     1981 tacctaaggg ttgatccaac catttctcta gtttttcttc taccggtacg atcgctgcaa
     2041 gaatttctgg atcaggctct gatttttcaa ctgagtgtaa agcaacctgg tgatctgtaa
     2101 caaccacttg cccctctttt ttctcgacgg taagacgaat ttcaccgatg aactcgccac
     2161 gatagcctgg ttgaacaacg ggaactccat gaaccgtttc agcaattttc cggtgctgat
     2221 gaccggtgaa aagggcgtca atgccttcaa cctgctgcaa tagctcgtag ccttcattct
     2281 ctccagttag cgcttctgtg tcctcaccgg ttaacaagtc tttttcaaat ccgccgtggt
     2341 aactaacgat taccaagtct gcttgtttcc ttaatagagg aacatattct tttgctgttt
     2401 cgacgatact cttaaacgta attcctgcta aggtttctgg cttttcccag tgaggaatgt
     2461 attgtgtcgt taatcccaag atagcaattt ttacacccac cttttcgata atcttatagg
     2521 cgtttccaaa gtatggttgg tcatttttgt tcaccatatt ggctcctaat ataggcgctt
     2581 gataattctt aatcgcttcc tcgagataat ccacaccata gttaaactca tgatttccca
     2641 acacttgaac atcatagctc atttggttaa tcaagtctgt aagctctttg gctgaaccat
     2701 attctttagc aagataataa ctcaaaggag acccttggat gaaatcacca ttttcgatcg
     2761 taattaccgg tccatctgct tttgcccgta attccttcag tttcgacgat actttagccg
     2821 ttcctaaggc cagatcggtt ctgccggaat agtctgttgg tgaaacatag ccatgcatat
     2881 cacttgtctc taaaaacgta atgttcattc tgcgtgctcc tttagctgac tcaaaataga
     2941 aaaaccgaag cgtattcagc tgttcggtct ctttttttga tttataatgc cttaatcctt
     3001 catttgaagt gtttttaaaa tgttgcctgg aataaagttt tctgttgtct ggcgaacaat
     3061 ccttccttga tcgagatgat aaaggttata cccatgacgt tcttcaaagc gtacgaagcc
     3121 atcgccttca tcttcaccaa caaaggacat actcgataca gtaaaatagg ggatcccatt
     3181 aatttcatca gtaaatgtgt gatgggtatg accgtttagg atacagcgaa tatcgtaagg
     3241 tgcgattagc ttcaagaaac gttcggtacc aggccatttt ggtatactgc tttggtgaga
     3301 cagtagatga tgatgggtga ccaagaaaat cggttggtct ttttcttgct ctaaggcttt
     3361 tttcagccat tgaaattgct gatcgtccac tataccatct gcgtatccat agcttgaatt
     3421 atcaaaggaa atcactgtaa actcaggata tttgactatc tggttgtacg gggcagaaga
     3481 ggcggcttca tcgcaccagc ctacccaaaa atgttctttg atatcgtgat tgcctagagt
     3541 aacaagaatc gtcgtttctc ctagcgcatt ttccagccaa gtcttcaagt atcgataatc
     3601 gtctatttcg ccatcttcgg ttaaatctcc gctgataatc acaaggtcca agggtgtttg
     3661 ctgcaggaga tgatgaatgc attgctccag agggataagg ggattctgca tcgtagccag
     3721 cattccctta tagccttctt cacaagcctc atactctctg cgaaagtgta tatctgatat
     3781 atgcaatata ttcattctta tctcaatctc tttcttataa ttccggttat ttgatctaaa
     3841 ataaacacgg ttacgactac gccaatcaaa ataatactca ctttgctcca agaccgagtt
     3901 tgaatcgcga atatcaatgg agcaccgatt ccgccagcgc caactaagcc taaggtagcc
     3961 gcactacgaa ccgctatttc aaaatgattt aatgctaaag aacaataaat cggcatcaaa
     4021 taggggatgc gaacaaagaa aatcgtttgc caaaaatttg ctcctaccgc atgcatttct
     4081 tcaacaagtc gctcatccat cgcttccatt tcttctgtaa aaagtttgcc aagcattccg
     4141 atctgatgga cgccaatcgc cataacacca gcaaaggggc caggtccgac gactttcaca
     4201 aaaataattg caaaaactag ttcaggaaaa gctcgtaaaa cattacagat aagcttgcca
     4261 atttttgtta cccatggatg tgcttgccag aggttaaccg cactgatcaa ggtaatggga
     4321 atcgctaata ccgtagcgat cattgtccct aaacaggcga ttccaatggt taataatagc
     4381 aaactaatca aatcctcgcc gctgccatca tatagaaact gccaatcagg ctgacctaac
     4441 cctctcaaaa cgtccattcc catggaagga gagaaggtgc ttaatccaga gtaatccaga
     4501 gtagcggccg acaaaataac gagaatcact aaagcaacta atgataaaag ggttcttctg
     4561 ttcatttcac gaagcatcat aacagggacc tcctgacacg ttcacttaat ttatcaacga
     4621 tcgcgacagt tactaggatc gttagaataa tgattgaaac tcgttcataa ttcaaaagcg
     4681 caagtgagga atttagaata acgccaatac caccagctcc gacatatcca aggacagtag
     4741 aagcacgaac attcacctca aacgcgtaaa aagcatagct cgcaaactga ctcaaaactt
     4801 gtggagcgat cgaccaaata gcgatttgca ttttatttgc tccaacagcc gcagcggctt
     4861 caatcgggcc aaagtcaatg gtttcaattg cttgaaacat taattgtgac accataccaa
     4921 aagtaaagac agcgatagtc aacacgccag tcgcttcacc aattccaaac acagcaacaa
     4981 aaagcgcggc taaaagcagg gtagggatgg tccgtactaa acctaaaaga aaacgaaaga
     5041 tggtagaaag aagacggttt tctgtcacta cagtggtcgc taaaaaacta acagggatcg
     5101 ctaaaattaa gccaatgacc gttccaagca aagacatttg aagtgttttc accataggct
     5161 cgactaattt aggtaaatag ctaaagtctg gatgaatgaa gcgtgataaa aagacagtca
     5221 tttgattgga gttttgaaaa acctgagaaa aatcagctga cgtgatccag gcacttcctt
     5281 gaaaacaaac aattaagaac agaagaataa gcaggtgttt ataccattta aagtgaacgg
     5341 tttctttcaa gctcatcgat ttcgcctgcc ttttcttcaa agattgcatt gccataaatt
     5401 tgctctaagc gttcatcggt taagtcgtct ggacgaccat caaagacaac ctgagcagct
     5461 tttaacgcga gcacacgcgt agaaaactca cgagccagag ggactgaatg cagattgaca
     5521 atgactgttt tatccatcgt ttgattgata ttttttaggt ccatcataac cttttgagtc
     5581 gtgattggat ctaatgatgc aacgggttca tccgctaaaa taatgggggc ttgctgcacg
     5641 agtgctcgcg caatggatac tcgctgttgt tgcccaccac tgagctcatc actgcgtgtc
     5701 tgcaatttat cagacaaacc aactttttcc agtgcttcct ttgtctgctg gtagtcttcc
     5761 ttcgtaaaaa gacctaaaat acttttccaa gtggagtaat agcccaaacg tccagatagc
     5821 acatttttct gaaccgtact acgctttacc agattgaagc tttgcgagat tagaccaatc
     5881 tggcggcgaa tctgtcgcag ctctttttta ttcgcttttg taatagaggt tccattaatg
     5941 cgaatatctc ctttggtggt ttctgccaag cgattgatac tgcgcagcag agtgctcttt
     6001 ccagcaccac tcaaccctat gatggaaaca aattctccat cttcaattgt taagtcaata
     6061 tccttcaagc caacaacgcc attgggatag accttcgaaa cattgtcaaa aataatcatt
     6121 atccttctcc atttttttaa gtgtaagcaa aaaaaagctt gctgacactt actgatgttt
     6181 tattctgctg cttttttcgt gtagtctctg atcgtgtcat aggcagaatc agcagcttct
     6241 tcatagccct gatgccccca caaagccatt gcttctttgc catctttatc atccttcata
     6301 tttaagaagg tctctttaat tttcgctttt aaatcttttg acatgtcagg ctgtacagcg
     6361 attgcgtcat tcggaatatc gccttttgtc aggtacagga cttttaaatc cttaaataaa
     6421 tcattatctg ggaaattcga agcaaaaaca tttcgcgcgc cttcaaaaac aaacgccgca
     6481 tccatttgct tgttcaacac tgcggtaatc tcactcggaa tatcattcac agtggtcatc
     6541 tttgtatctg cagtaggatc aatcccagca tctttcaatt cagcgacagg atagatgtag
     6601 ccgctagcag agttaggact taaggttgcg atattttttc cttttaaatc agttaactta
     6661 ttcaatccgc tatcggcacg aaccaatact tctcctttaa aggtgttcgt aagtttgtcc
     6721 tttaatggca agccagtttc ttgatcataa tcgccgagct gagaagtcag gattgcatct
     6781 gcagctccag catcctttgc ttgaacatag gctgcaggcg gcataattcc gacatcgact
     6841 tgaccagaag acatcgcttc gacgatcgtt gaataatcag tagctaacgt cacttctacc
     6901 tcacgatcta actttttcga taaatactta gcaaatggtt ttgcttttgc ctccatacta
     6961 ccatcattgt ttgttggaac gaattgaaca actaattttt ctttgctctc tttttctgag
     7021 ctgctacttg tattcccaga accgcaggcc gccaaagtgg tcaataacat gactccgatg
     7081 aacccttttg ctaactgttt gaactgcatt tttctcctcc tgttggatat aatttctaat
     7141 acgtctaaat catagcaggg aatcaaaatc aatgaagtga cgcacgtcct attttacaca
     7201 acttttacat acaatttaca aacaattata aataattatt cgtattgagc cgaaaaacaa
     7261 tctaagctat aataaagaaa aacttcttag aggtgacgat ttcatgaaaa aaatccatga
     7321 tacgaagcaa ttaactgctt acgttgagaa gcacaacctg caggattata tggattcaga
     7381 tatattggca atggcttctt tacttttttt tgaaaaggaa gagcacctca ttcaaacggg
     7441 aaaaacctca gattatttgt attttttagt ggatgggacg gtaatggtgt actcgtattc
     7501 ttctgataca caaaatattt gcatcgatta cgcaaagccc gctacaccac tcggcgaagc
     7561 ctcttcattg tgggaattat cgccgaaaag cagcgttaaa gcagtgacta agtgcatctg
     7621 tatcagcatt ccgctaaatc aatatcgaac acagcttcaa aaagatgttc gctttttgca
     7681 gaatatttgc cagttactaa gttatcgttt aaattccggg ataaatttgg cgaactcact
     7741 gacagaacct gttgaaactc gtttagcaaa gtttattttg acccatcacg aaaacgaacg
     7801 tttctcattt cggttaacca cctgcgcctc aatcttaaat gtcagttatc gacatctttt
     7861 acgaacaatc acgaattttc gtgaggagaa tattatcgaa aagaaaaaga attattactt
     7921 gattcatgat atcgaagctt tagaggactt agctgagaat ataagtagtc cagtaaaagc
     7981 agaataacag ctataaaaaa acagcagtca gaagtgattg ctggtttttt gatagtggag
     8041 aaattgggaa acggaaaatc ttcgttgaag agtagtgttc tatatattgg tatgctatac
     8101 ttttaaaagt tcaatgtgtc aagacacata aaaaggggag aaaaattttg aagaaaatac
     8161 ttgtttcatt gttgtttttg gtgtcattag taacgttagt cggctgtagt cagtctcagg
     8221 gggagaagca aagtgaaaaa gagaaaagta aaatcgcttt ggttcttgga tttggcgggg
     8281 ttgatgaccg ctccttcaat caagctggct gggaaggatt agaggcttgg ggaaaagaga
     8341 aaaagctgac agcaaaaaaa gattttacgt atatagaaac gaacaatcca gaggataaca
     8401 gtaccaatat cgatcaggca atcagaaacg gctttaccac cattattagc ttgagctact
     8461 tgcaagcaga ggaactggaa aatgctgcta aagataatcc aaagttgaaa tttgccttgg
     8521 tagatggtga gattactggg aaaaagaata tctcatcagc aacctttaaa gatcaagaaa
     8581 gtgcgtattt agcaggacta gctgccgcat atacgacaaa aagtgatcaa gtaggcttta
     8641 ttggtggaat ggaagggccc gtgatccaac gtttccaaag cggatttgaa gctggcgtta
     8701 aggcaggggc cacagcactg aacaaggaaa ttactgttaa cgtacaatat gctgcatcct
     8761 atgattcgct agataaagga aaagctattg ctgcttctat gtacaaagag ggagtcgata
     8821 ttctttatca ggcggcagga agtactggag ctggggtgtt ccaagaagca aaagcccgca
     8881 atgaaaacga ctatagcaga aaagtatggg tgattggtgt agatcgcgat caagcggatg
     8941 aaggggatta taaaatggag aacggcgaga cagggaattt cacgttgact tctacaaaga
     9001 aaaatgtcgg tgcagaagtc aaagaatttg cagaaacttc tcataacgat aaatttcctg
     9061 gcggcaagca tttgaccttt ggtttgaaag aagaaggggt agatctgaca aaaggacaat
     9121 tatcagagga agcctatcag gcagtttcag acgcaaaaga gaaaatcatc agtggtgatt
     9181 tcagactgta gacaaagtcc attttatgga tga
//
LOCUS       DASJON010000114         9152 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJON010000114 DASJON010000000
VERSION     DASJON010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.115"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..609
                     /locus_tag="U3989_001953"
     CDS             <1..609
                     /locus_tag="U3989_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN1715399.1"
                     /translation="KDSIRRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETF
                     LSVEQLFKDVAIERGYLYAGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFD
                     LEQVARVWNHGSVIRGWLMEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVP
                     VPVIATSLFMRNLSQEEDSFSAKVVSALRNGFGGHEIVEKGE"
     gene            611..1612
                     /locus_tag="U3989_001954"
     CDS             611..1612
                     /locus_tag="U3989_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN1715400.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1609..1893
                     /locus_tag="U3989_001955"
     CDS             1609..1893
                     /locus_tag="U3989_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN1715401.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            1926..2723
                     /locus_tag="U3989_001956"
     CDS             1926..2723
                     /locus_tag="U3989_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1715402.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            2737..3570
                     /locus_tag="U3989_001957"
     CDS             2737..3570
                     /locus_tag="U3989_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715403.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3588..4445
                     /locus_tag="U3989_001958"
     CDS             3588..4445
                     /locus_tag="U3989_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1715404.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4467..4976
                     /locus_tag="U3989_001959"
     CDS             4467..4976
                     /locus_tag="U3989_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715405.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5013..5414
                     /locus_tag="U3989_001960"
     CDS             5013..5414
                     /locus_tag="U3989_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715406.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5526..5879
                     /locus_tag="U3989_001961"
     CDS             5526..5879
                     /locus_tag="U3989_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715407.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            5913..6356
                     /locus_tag="U3989_001962"
     CDS             5913..6356
                     /locus_tag="U3989_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1715408.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6373..7458
                     /gene="uxuA"
                     /locus_tag="U3989_001963"
     CDS             6373..7458
                     /gene="uxuA"
                     /locus_tag="U3989_001963"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1715409.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7451..8164
                     /locus_tag="U3989_001964"
     CDS             7451..8164
                     /locus_tag="U3989_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1715410.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8371..8520)
                     /locus_tag="U3989_001965"
     CDS             complement(8371..8520)
                     /locus_tag="U3989_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715411.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            8916..9116
                     /locus_tag="U3989_001966"
     CDS             8916..9116
                     /locus_tag="U3989_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1715412.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3068 a         1442 c         2039 g         2603 t
ORIGIN      
        1 aaagattcaa tccgtcgagc agaagagttt cagaaaaatg aaatcggtta cttggattgt
       61 gggacttctg gaggaatcga aggagcacga catcaagcct gcttaatgat tggaggagat
      121 aaagaaactt ttctatccgt ggaacaatta ttcaaagatg tagcaattga aagaggatat
      181 ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa tgatccataa tggaatcgaa
      241 tatggaatga tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag tgagtatgct
      301 tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg cggctggctg
      361 atggaaatca tggaagatca gttaaacgaa catcccaatt taagtaatta tcggggaatc
      421 gtttctgcat caggtgaagc aaaatggacg gttgaaacag cgctagaaat ggctgttcca
      481 gttccagtaa ttgcaactag tttgttcatg aggaatcttt cacaggaaga agatagtttt
      541 tcagcaaaag tagtttcagc actaagaaat gggtttggcg gacatgaaat agttgaaaag
      601 ggtgagtaaa atgccagatt atcatatagc aatcgttaat tccagcagtt tcggaagagt
      661 ctttccacac cacttggaaa gattaaagtc agttggagac gttcatttct ttactgttga
      721 tcaagagatt ccaggaaaag aactagcgga gttacttaaa ggattcaata tcattattgc
      781 aagtgttacg ccattttttc gacgagattt ttttgaaaac aaagatgaac tgctattgat
      841 ttcccgacat ggtattggtt acaacaatat tgatttaaaa gcagcaaaag aacaccagac
      901 aatcgtatct atcatacctg ctttagttga acgaaatgct gtagcagaaa ataatataac
      961 taatttgttg tccatgcttc gacgcacgac acaagcacaa gcaagagtca aggacgatcg
     1021 atgggaagat cgggcggagt tcgtcggtag gacgctgttt aacaaaacag ttggtgtgat
     1081 tggtgcagga aataccggaa gcggagtaat cgaaattttg cgtaacggtt ttcgctgtga
     1141 tgttctagct tatgatcctt acaaatcaga tttatacatc gaaagctttg gtgcaaaaaa
     1201 agtagaactt caagaattac tggaacaatc agatatcatt tgtttatgtg cgaatttgac
     1261 agaagagaac tatcacatga tttctgataa agaagtcgat cagatgaaag aaaatgttta
     1321 tctatctaat agtgcaagag gcgctctagt tgatgaagaa gcgatgatta atggtctgaa
     1381 aagcggaaag atcgcaggat atgccacaga tgttttagaa gaagaaccgg gacgtcaaga
     1441 tcatccgtat ttagcgtttg aacaagtagt gattacgcca catatatcgg cctacacaag
     1501 ggaatgttta gaagcaatgg gtgaaaaatg tgtcagagat gtcgaacaga tcagtcagca
     1561 ccttcttcct gaacgttctg tccagacaaa gagcaactat ataaaaggat gatgaagaaa
     1621 atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt atttatatga agaaggtggt
     1681 atttcaaaag tttctgatct cttgaaatca tacggcgcga aaagagtact ggtcacccac
     1741 ggaacggtat catgggaaaa ggcgctacct aaattagtat ttttgaatga cgaaacgatt
     1801 caattttttt atcatcgtta ttctggagaa tgtagttatg ctgaagccag acgaatagct
     1861 acaatcatca aaaaaatgaa atcgatttcg tagttggcgt tggcggagga aaactgaccg
     1921 atctagtggg ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc cctactttag
     1981 ctagtaattg tgctccatgg acacctcttg ctgtcatgta taaggagaat ggacaagcag
     2041 aagggaaaac agaacacttt tacagacaag ctgctttttt tataacagat ccccaattag
     2101 tgattgattc ccctgttcca tatttcattg ccgggttagc agatacacta gcaaaatggt
     2161 atgaatctga gctcatctta gaacaagatt ttttgcaaga tgaatcattt ttgaagctag
     2221 cacaagatac tgcgaagatc tgcaaagaag agattttgaa tcattctgct aaagcaatcc
     2281 aagatatggc acaaagaaaa ctgacatcgg agttcaaaca gctttcagag attatctttg
     2341 ctgttgctgg tttagttgga ggattaggag ataaatacgc aagaaatgtg gcagcacatg
     2401 ctatgcatga cgctatcagt aaatatattc cggaaagcca tcgttttcta catggagaaa
     2461 aagtagcgta tgggattttc tatcaactgg cactagaagg tcgctgggaa aaaatagacg
     2521 agttatatcc tttgtatcgg gaattagaac tgccggtttc tctagaacaa atgggattat
     2581 ttccgaaaga taaacagata atagataaaa tggctgaatt tattgattcg aaagagaaag
     2641 tgcatttgat tcctatagag gtaacacaga aatcactgat acagtcaatg atggaactgg
     2701 aaggttatat cagaaagatc tgaaggagga ggacacatgg aaacattaag tgtagtacaa
     2761 agtttgttga ttgctttatg ggtagccgcg gttatgtccc gctggttagg tggaggagcc
     2821 acgttgacct tacgtttttc tcctttgatg actggattag tcgtcgggtt gatcatgggg
     2881 gaagttgcac aagcgatgat tgttacagcc gctttgcaaa tgatttatat gggggttttt
     2941 tctccaggag gatcaatgcc agctgaacca tctattgcag cagctatcgc agtaccagtc
     3001 gcattattag gggatctgtc gccagaagca gcgattgccg ttgcggtgcc agtcggattg
     3061 ctcggaagtt acttgtatca gtttcgtttt ttcatcaata cttttttagg taaatataca
     3121 gacaaagctg tagaagattt gaatacaaaa gctatggccc gatcaattat tctttatcca
     3181 acggttgcct catttttatt attcgttccg cttgttttca ttgctttata tttaggggcg
     3241 ccattgatcg ctgatgtaat caaagcttta gagggaacag tcgttatcca cattcttgaa
     3301 gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt tatcggtcga
     3361 aaagattatc tggtcttttt cttccttgct tattttatga gtgttgtttt gaaatcttta
     3421 gatattacga tggttactta tgccattttt gggattatta tcgctttgat ctttgtccag
     3481 tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata acaattcagc atcttttgat
     3541 gacgaagatg actatgatga cggattttaa tttaggagga atgggaaatg gctgaaataa
     3601 tggataatca aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca aaagcctatc
     3661 tgagatggca ttttgcaaac gaaattcctc actcttttga acgttacttg gcgccatcac
     3721 tactatacgg aatgatgcca atcttgaaaa aactttataa agatgaagac tctttaaaag
     3781 cagcatataa acggcagtta ctgttcttca acactcaatt aagctggggt ggcggtgtga
     3841 ttactggcct aatgtcttct atggaacagc aaagagcaga agaagagtat gaagaaaaag
     3901 agatcttgat gcaggatgac ttgatgtata acacgaaagc gggattgatg ggggcactag
     3961 caggaatcgg agatgcaatc gattcaggaa ccgtccaata tatttttatc gcaatcgcgg
     4021 taccttgggc acaacaggga agtcctttag gcgcactttt tccttttatc tgctttgggt
     4081 tgtatcaggt tttactaggg gtatttttcg caagacaagc tttcactatg ggccgtaatg
     4141 ctacaggcct aaagcaaaac actggtgttc agacagcgat tgaaatgcta tcagttttag
     4201 ggttgtttat gatgggggtg cttgcgggaa actatgtaaa agtagaatct tcattgaaat
     4261 ttgcgatatc cggacgtgag tttgttatcc aggaaattct tgatcaaatc gtgccaggga
     4321 tattaccttt agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa ggattgaaag
     4381 tcacacaagc attattatgg ctgactggaa tattaattgt gctagcagga atcggtattt
     4441 tataaaaaga agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt gtggatgagc
     4501 gtttgatcca tgggcaagta atggtaacgt tatctcaaaa aagtggagtg aattccattt
     4561 ttgttgtaga cgaagttgta gcaaaagata aatttatgag agatctttac aaaaatgcag
     4621 gaagccgtac aggacaaaaa acaattgtca ttacaccaga gaaagctaaa ttctattggg
     4681 atgaatatca atttaaagaa tacaattgta ttttgatcgc aaaaacagtc caaacgattt
     4741 acgacttagt aaaacacgga atccccatgg aagaattgaa tattggaggg attgctcaaa
     4801 aaaatcctga tacagatctt ttagtgacga aatctgttta tttaaataaa tcagatgctg
     4861 aaaaattgaa agaaatgaat gaacaatatg gcgtaaagaa tatttatttt caagccactc
     4921 catctgcacc taaaacagga ttaaaagatg tattgaacaa gtttgatttg tcataaacaa
     4981 atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg tttcgtgatt
     5041 gggaatttcg ttatcgctat atttaccgta agcgccgtac aaaaaagagt aaacagcgtt
     5101 ttctttcagc attagtatca gatatttaca gtatgagaac agatgtgaca gtcattgctt
     5161 acgatacgct agcctaccga tcaaaaaata tttatgttgg agatatcgaa aaagcagaga
     5221 aagttatctg tacttattac gatacacccg ttcatgcatt aggaagttat ttcatgtttg
     5281 attggaaaga ccaaaggaaa aaaacaatct actctatatt attgtccttc attttgttgt
     5341 tcagtttagg ttggtgggga atgatgatat ataacaaaaa tcctcatcac gtattcgatc
     5401 tgctatccgt ataaacaggt atcactgtac tagcatttgg gagctacttt ttcttattgg
     5461 gaaaagcagc tcgcggatgg cctagcagac aaacttttat tcgaaatact tcatccatat
     5521 taacgatgct agaaatgatc cgtacgattg atgatccgag tgtagcgtat gcgtttgttg
     5581 atgaaggctg ttatggagaa aagggcttag acagcgtgag atcgggtatg aaaaaagagg
     5641 ctattctttt ttatctagac tctgttgggg cagatacacc gctacagttt tcgggaaatt
     5701 atttttcaaa taaagaacag tggctgaaac aagttgataa gttgaaagaa aaaaacgtca
     5761 attatatttt tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc aaaacagatt
     5821 tacgagggaa aacatttaat tggcaaaatg ccaatcagat tattgctctt tttcgataag
     5881 taaaaagtgc aaaacaagga gtgagaagac taatgttgga aatcatgatc gttacacatg
     5941 gtaagttgag cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca tcgagccata
     6001 ttgaaacaat gtgcttagag tctggggagg acatccaaac attcggagag aaggtcaaag
     6061 aaaaattaac tatgatcaac acagcagaac ccatgctagt atttgttgat ctaattagcg
     6121 caagtccgta caatcaagcg ctcttggcag tcaatgaact gactgaagaa aagaaagcag
     6181 ccacttatgt catagggggc gtgaatctgc caatgttgtt agaagcaatc aatcatcaga
     6241 tgcttcacac accgatagaa gaagcggtgg aacaaattgt caaacaagga aaagataatt
     6301 taactgtttg gcatgcttca gcggaagaaa aaaaattaga tgaggacgat ttttagaagg
     6361 aaggctgaaa agatgaagtg gggatttaga tggtatggtg aaaaagggga ttcgatccca
     6421 ttaaaatatg ttcgccaaat accgggaata aatgggataa ttggaacttt gctgactaaa
     6481 atgcctggag atgtttggga gatttcagaa attgaggagt tgaaagcatc tgttgagagc
     6541 caaggactgg aactgcttgg aattgaaagt gtagcgatcc acgatgcaat caaggcaggg
     6601 acagatgaac gagattacta tattgataac tatatacgaa caatcaaaaa cttgtctcga
     6661 tgtggggttc gattgatttg ttatagcttc aaaccaattt ttggatgggc gaaaactgat
     6721 ttgtttaatg aaaacaacga tggaagctat tctcttttat acgatcagaa aatcgtcgat
     6781 cagatggagc caggagaaat gtatcaattg atacacagcc agtcaaaggg attcaaattg
     6841 ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact gtatgaagga
     6901 atgaccgaag aagaattatt tgaaaatctg acttattttt tagaaaaagt gattccagtg
     6961 tgcgaaaaag cagatgtaaa aatggctatc catccagatg atccgccatg ggaacttttt
     7021 ggttttccta gagtgacgaa aaatcttggc gacttgaaga aaattctaaa tgcagtagat
     7081 tctccatata atggactaac gctgtgcaca ggatctttag gggcaaatcc ggaaaacgat
     7141 ttagttgaaa tcatccatga ggtaggttcg cgcatccatt ttgtccactt cagaaacgtg
     7201 gagtttttag gagaaagaaa gttcaaagaa acggcacatc cctctacaga aggctccctt
     7261 gatatgtatc atattatgaa agcgttggtg caagaagggt tcgacggtcc tatacgccct
     7321 gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct gtatgatcgt
     7381 gcaatgggac tcacttacat gcaaggtcta tatgaggcga taagtaagga aaaaccactg
     7441 ggagagcatt atgaataaaa tcaagctctt catctttgat atggatggcc ttttattaga
     7501 aactggacgt ttagcttatc gtgcttatgt aaaatcagca caaaagtatg attatgaaat
     7561 gcgtaaggaa gtctattatt tactaactgg ccaaacagaa atggcgataa gagaacaaat
     7621 gggcattttg tacggagaag atgttccata tatcaaatgg cgagaagcaa tcaatcaata
     7681 taaagaaaaa atcgtgaaag aagataagag agtatatacg aaaaaaggag cagaagaaat
     7741 tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta atacacgggt
     7801 aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc aaatcatttc
     7861 aggggatgat gtaaaaaaag gaaagccgga accagagatc tttttgaaag cttgttcaaa
     7921 aatgaatatt cctccaagtg aagcacttgt atttgaagac tcgattgcag ggatcgaagc
     7981 agcaaggcga gcgggcattt tgtcttttct catcaaagat catttgatag ggttgcccga
     8041 tcataatggg aagcataagt tgaaagtaga tgtaagttta tatgaatcca ataaagcaga
     8101 ctatgaattt tatgacctgc atcaagcaaa acgattttta gaaagtaaag ggttgtatct
     8161 ctaataaaga ggactatttt ctagtcctct ttatttattg tcctgcacga ctgagggaac
     8221 agatgaaaaa gaagtaattt tttattttga agtatctacc ttatttgtag tgaacaattt
     8281 agaacaaaca caaaacgcat ctactgttat ctaccagtag atgcgtttaa agagaagaac
     8341 gagaatcgtc catatgaggg tttccactcc ttatgcagat ttttttaatt cttgcactgc
     8401 ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa aaacgacaaa
     8461 tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat agaatgtcat
     8521 agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga taacgaaatt
     8581 ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt taaagtcaaa
     8641 agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa
     8701 ctttttcttc gattcgaaga caatagaaga caatgaaatt tatatttcat tgcattatcc
     8761 ttgtgttatt agaaaaagca tgatatatta aatgagtaat ctattttgaa acgtaatttg
     8821 agcgatatat ctacatagat aaaactcctt ttgcctatca aatatttatt gcaacgaaaa
     8881 aacgtacatt gatacgtatc aggaggaatt tatttatgaa taacggtaca gtaaaatggt
     8941 tcaacgcaga caaaggtttt ggctttatca ctggtgaaga tggaaatgat gtatttgcac
     9001 atttttcagc tatccaaggt gacggcttca aaactttaga agaaggccaa gcagtgtctt
     9061 acgatgttga agatggtcaa cgtggccctc aagcaacaaa cattgttaaa gcttaataaa
     9121 aattgacttt taaccgtctt ttcgaaagaa aa
//
LOCUS       DASJON010000115         9146 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJON010000115 DASJON010000000
VERSION     DASJON010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.116"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..637)
                     /locus_tag="U3989_001967"
     CDS             complement(<1..637)
                     /locus_tag="U3989_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715413.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALK"
     gene            763..1144
                     /locus_tag="U3989_001968"
                     /pseudo
     CDS             763..1144
                     /locus_tag="U3989_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1195..1611
                     /locus_tag="U3989_001969"
     CDS             1195..1611
                     /locus_tag="U3989_001969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715414.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            2029..2799
                     /locus_tag="U3989_001970"
     CDS             2029..2799
                     /locus_tag="U3989_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN1715415.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            2803..3441
                     /locus_tag="U3989_001971"
     CDS             2803..3441
                     /locus_tag="U3989_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715416.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            3818..4729
                     /locus_tag="U3989_001972"
     CDS             3818..4729
                     /locus_tag="U3989_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1715417.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            4946..5383
                     /locus_tag="U3989_001973"
     CDS             4946..5383
                     /locus_tag="U3989_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715418.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            5478..6362
                     /locus_tag="U3989_001974"
     CDS             5478..6362
                     /locus_tag="U3989_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1715419.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            6584..>9146
                     /locus_tag="U3989_001975"
     CDS             6584..>9146
                     /locus_tag="U3989_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715420.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         3087 a         1461 c         1859 g         2739 t
ORIGIN      
        1 tttttaaggc atcgtcatag tcttggatga ggatttcttg gtaatctgag ggaatggctt
       61 cccaagtgag caggcgaacg ttcatgacgt gttttccttt gtgtttgatt acagcatgat
      121 tcttaccttt ttctacctct ccagcgtgaa aagacagcca tgtaccatga acaaagatat
      181 cattacatac ccaatgttcg atggaatttt ccacgtatcc tacagggagt gtggtatagc
      241 cgatttgtgg tttcaaaaaa ggattgaatc gatcaaccgt ttcattttta tattgataat
      301 tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt gtctttttga
      361 gtgagaaggg ttgttttgat gaataagatt tcttgataac aagaggttca tccggcaatg
      421 ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc agacctaaag
      481 tctgatgtaa aagtgacagc atttcaaggt ctggcattgt acgagaattt tcccaactag
      541 agattgtttt atcggaaaca ccgatttttt cgcctaattc tttttgcgta agcttacaat
      601 ttctgcggta agttcgtatt ttttctccga tgttcatctt cttctccttt cgattctaca
      661 gtatatagat ttattctaca tgacctatac atttgataga tacgtttttt tgatgaaaaa
      721 cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat caaaaaaaag
      781 ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc cttggttatt
      841 ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga tacttatact
      901 ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct aatcatggta
      961 tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt aaatgaatcc
     1021 aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat gacattgtat
     1081 tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac ctcctttcgc
     1141 gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt tttcttgatg
     1201 acgagagaaa aagaagccta tgaacggttc attttactat ttgtggaacg attcaatcaa
     1261 gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag ctattcgtat
     1321 tcggttattt caagtacaga cgtatataat tatcgagtgt atttaactat tggattgatt
     1381 cttttattcc ttttgttatc atcctttttc ttattgatga ataaagaaat cagtttattg
     1441 ttgaataatg gcttttcatt acatcaagta ttgtggtgtt tattggggaa aaagaaaatc
     1501 attattacat tcataattgc aggaatcatt ggtggtttta tttttatgta ttataaggag
     1561 caatttcttt atttgttcca gatgtacgtt ttcttgttca gcggaatata gggtatctct
     1621 ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt caactatatc
     1681 caatacattc tttttacatt tggattgttg ttttctcttc ttttgaccgt tgccaatgtc
     1741 gaattagcaa ctatcgtttt ttcttcttca cagttagctg tttcccatga aacaccaaaa
     1801 gaaatcgctg gtgaggacta tcatgtattt tatccagtaa ccatcgggaa aaaccacgtg
     1861 gattttattt attccaatcg ttttgcatca gcagccgatc aggaattata tgaaactttg
     1921 aataaaaacg gaagtatatt agtcaatgta tcagattact tgaatgaaga aaatgaacag
     1981 tttggcagag ggataaaaat aaatctgaat tatcttaaga aatttccttt gattgatgtc
     2041 tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt gatccctgaa
     2101 cgatatagac atacagattt aaaaaatgac ctggcgcaaa taacagaata ttatcaagaa
     2161 atcagtgaaa aagaaccgaa atttctattt ataaaaaaca atcaacccat ttatacattt
     2221 attccttcta tcccctggat cgaacattat ccggttgttg aaatactgac attgaaaaac
     2281 agtacgtatt gggaaagaaa tattctcagc ggagaaatct atccaccgtt aaagatcaag
     2341 ataggctctt tgtcgaaaga aaggttattt gagctgattg aagaaagtca gttacgtgat
     2401 aacttgcagt tatcatttcc gtatactcag actgaagaga tagctgtcaa agtgttaagc
     2461 agatcatttc attacttgat acagatattt ttactcactt tcttttcttt cttcttgttg
     2521 agctatgtca tgttatgcat atattttgtc tacaattgtc ggaaaatcgc tatctttcgt
     2581 tcaagtggtt attcgctctt tgaaacgtat aaagatttct tcatgatgaa cctcatcaaa
     2641 tggggaacga caagtgtaat cttccttttt cttatagaaa gagagcctaa atatcttttt
     2701 aatatttttt tctttagctt cattgatttt attctttcag ttgtttttat tttgtctaca
     2761 gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac aattggagcc gttgatagtg
     2821 ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga tcgtttcact
     2881 gcggatgctg gggattttgt gactgtagtt ggagagagcg gagcagggaa aagtacttta
     2941 ttgaatatat taggattgaa tgatcaattc agttcagggc aattttattt atttaatcaa
     3001 gaaacaaaac atttaactgg aagagagaag cgcttgataa aaagaaatgc cctctctttt
     3061 ctttttcaag attttgggtt gattgaatat gaaacaatcg attttaattt agatattgga
     3121 ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag caaagtatga ggcattgaaa
     3181 aaagtaaata taaacaagaa gctaagtacg cctatctaca cattatctgg tggtgagaag
     3241 caaagaatcg cctgtgcgag agtcttattg aagaatagca aaatcatttt agccgatgaa
     3301 ccaactggat cattagataa acgaaataag gagcaagtcg ttgaaatctt atcgcaacag
     3361 gcttcccaag gtacgttagt tatcgtggtc acgcatgatc catatctgac caaaatagga
     3421 aacaagaaga tcatgcttta gaaaacaaat gaaaaaacaa actttttcta ataaaaacaa
     3481 caaaaaaatg tctggaaaat cgaacataaa agtgtctgat ttgaatatcc caacagcgga
     3541 agaagaaaaa caaaatcgat aacatttgtt ctaagaatta gttacgttaa atacggaacg
     3601 caagatagac ttgaaaaacg gtaacaaaag aagtagtata ttaaggtgtg atgtgctact
     3661 tgacatttat tctgctgagc gaactttctg gattgtgtag ccttatatct attcaggacg
     3721 tatcgagcag attagtaaag gtgttcttga tgattttcat cttgaacacc tttgttttta
     3781 gcaattcgca aaaaacgaaa agtaggagaa ttgataaatg aaaaaaagat ttttagcact
     3841 tgccatcgta ctaggcactg gcttattgtc aggctgtacg aatgccggtg aaaaaacagc
     3901 cgttagttat aaaggcggta caatcagcga acaagaagta atggatagct tgaaaaaaat
     3961 gcaaggagca gattccgctg tgcaacagtt gatcgtttat caagtatttg aagataaata
     4021 tggagatgac gtatcaacca aagaaatcga ttcccaatat gatcaaacaa agaaacaatt
     4081 gggtgattca tttgattcac aattgaaatc tgcaggttac acagaacaaa cattcaaaga
     4141 cagtatcaaa caaagtctag cctttcaaga aggtctaaag aaacatatca aattgaccga
     4201 tgaagattta aaaacggctt gggaatcttt ccatccagaa gtggaagctc aaattatcca
     4261 agtagcaagt gaagatgatg caaaagatgt gaagaaagct gctgataagg gagacgattt
     4321 ctctaaatta gccaaagaca agtcaactga tactacgaca aaagaagatg gcggaaaagt
     4381 gaaatttgat tccactacta caacggttcc ggcagaagtc aaagaagcag cctttaagtt
     4441 gaaagatggt caagtttctg atgtgatcac ttctaccaat gcttcaactt atactacgga
     4501 atattatgtg gttaaaatgg tgaaaaacca aaacaaaggc aacgacatgg ataaatacaa
     4561 aaaagaattg aaagaaattg caacagacac aaaattaagt gacagtacct tccagaataa
     4621 agtaattggc gaagtattga aagacgctaa tgtaaaaatc aaagacaaag actttgaaaa
     4681 tgttttgagt acattcactt cagattctag tacagcaagt agtaaataag cggtataaat
     4741 aaaaaatatg aactatttaa atcaataaaa ctgttcatct aaatcttttt attagaacta
     4801 aatcaacagt agtttaatta ttacttgatt tagttctttt tattttaaaa atttttttta
     4861 cttagaatcc agccacccta gatatttttc tgtataatct taaataaaga aaacgatatt
     4921 aaaaaatgca cgtagaagta gataaatgga gtggttttgc aaatttcttt tatttctaga
     4981 agttattcac gtatacggat ttgtttttct cacaaataaa aaggcaggaa gtacaatgga
     5041 tcatttgtat ataaaatata ttcatcaatt ttccgttttg gtattaggca cactaattat
     5101 agcggggatt gttgcattta ttattcaagg tcgtgacata ttaacagtac atcaagagtt
     5161 tttcagttat gcatttggta ttcctctgat cgctataagg tctgataaca aaaagtggaa
     5221 gaaacgctat tacattctct tgctattttt gtgtttttat ctgccaccta agatttttac
     5281 tttattaggt atacaatgcc tatcattttc ttttatttcc tatatcattg ttttaatttt
     5341 cctcagtttc atagtatggg tattttatct tgtcaaaaaa taaatgaatg tttcatgaac
     5401 agtacttcat cacagcctaa tttttttctt tatgctatcg ttatattaaa gaaatcaatc
     5461 agaagggaaa gaaagtgatg aaaaaagttt tattagtggt aaaccccagt tcaggcggtg
     5521 aacaagcaaa ggaatttgag caattagcga tagcaaaatt ggaatcggtg tttgatgaag
     5581 tggtcgtctt gcatacaaaa aaagcagggg atgcaaaaaa ctttactcgc gaagcggcta
     5641 cggaagggta tcatagtgtg tttgtcatgg gcggagatgg aacagtcaac gaaggaatca
     5701 gtggaatcgc tgaacaagaa catcggccaa atttcggatt tttcccattg ggtacggtga
     5761 atgacttggc tcgagcatta gggataccgc tagaacctga ggaagcgatc aatcattttt
     5821 cgattgaatc agtcaagcca ttggatattg gaaaaataaa tgacgactat tttatgaatg
     5881 tcgtcgcaat tggctctatc cctgaagcaa tcaatgatgt agatgcggag aagaagacga
     5941 aatttggaaa actagcttat ttcatgtcag gaatcaaaca gttagcttcc acacaatcct
     6001 actcgtttca cgtagaagtt gatggaaaga aggaagaaat tgagagcagt atcttgctga
     6061 taggactaac taactcagta ggcggttttg aaacattact tccaaatgca aaagtggatg
     6121 atggtaagct ccatttggtt tatttaaaag attcttcttt gatcgatacg cttaaaacat
     6181 tgccagacct attaaaagga gtagatgaat ctacgaaaaa tctagcctat cttacttgtg
     6241 agcaattagt tgtgtcatta gctgatgagg aagaattgac gacgaatgtt gatggagatg
     6301 aaggggataa attacctgtg acaatcaaag tgttaccctc acatttacag gtttattgtt
     6361 aaaatggagg ttgtgaccga agagttgtca ggaggacatc ggttgttcgg aataagggac
     6421 tgtgacaaga cttttgtcac agcctttttt tgtacaaaaa agaaattttc acctaacaga
     6481 aaaatttgcc ataaaaatac atattttaaa tatgaaagta caattacaaa taaattaaaa
     6541 cgttaaaata aaattgtcaa aacgtcagag aggatggtgg agaatgaatc aatatgtaaa
     6601 aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg atggtgacat ccgtttttgc
     6661 cgatgtagag aaaacaagcg aatcaacgca atcagagaat caaagttttg acaagactac
     6721 tttttcagaa gaatcaatta cttctgaaac tacagaatcc caaagtgagg cgcctacttc
     6781 taatacaagg caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc
     6841 tgatagcagt gaagaggtac aggttagagt tgtttcagcg gatgggtaca gcgaagctgc
     6901 cacaacagaa gaactgacgc agcttctcgc agacgaatca gtagctaaga ttcgtttaac
     6961 tcagcctttg actttagaca gggaattgga gatcaagcga gatatcgtta tcgattttgg
     7021 cggacttgct cataatttcg gcacacatca catctatatc aatgagacac ctcagtcgat
     7081 cgagtttcag aattttaaag gaacggcagc tcatccaggt gggttgatac caaatatcga
     7141 tggaaatgcc attatcattg cttatatgtc cgatttttgg ggaaacagat atcattttac
     7201 gggagaaata aaatttaccg ggacacttga cttatatgat ggttctaaat taggcctgat
     7261 ttacgcccct cgtgcaactg tcacattaga tggtgtttcc ggtgtattgg atgtccaacc
     7321 agtgaaagaa gggatgaatg ccgctccggg taaagcttat ttttgtagaa gctatcagct
     7381 aaatgtaatc aatggtagtc agctttatgg accatatcta ggaaaattct atggttttct
     7441 agattcaggt gatgagacgg gcagtggaaa aacagcacca ggactccata tcttgagtgg
     7501 ttcaaaggtc tctcttgact atgatcgggc agatggcgtc agcgatgaag gagaggctgt
     7561 cgatacgcta cccgggaatg tcacatttaa agtcagtggc tcaggaagtg aattttcggt
     7621 aaatacaaag atcaatatta ccaacgacaa caatcgcgga attatccaaa tgagaggctc
     7681 aggatcaaga gtcgctgtgt cgaatgatgg gaaaatcaca atcaatacag cgactactgg
     7741 cggatttcgc ctgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc
     7801 ggtcaaaatg gctggagatg gcgaccaacc tggaaacaat gggatgcgtt ttgtagggaa
     7861 aggactttct ttgatcgtag aaggagccgg atcagtcatc gatattgata agcaaagtgg
     7921 tcgttctagc gcagttcgtt ttgagaacgg tacgcaaaaa ctaacagtaa ctaatggagg
     7981 ctcattgaag gtaagaaatg caggggatgg caaatcttat gtgaacagac tcaatcaagg
     8041 aaaccaagcg atccaatttt atgacgcttc tggttttcta gaaagtccag ggtcagctga
     8101 ttttacggtt actggagaaa aatcaaatat cgatatacgc gcagattccg gagctaccgt
     8161 agatacaact ggagatattg atttgaactt tatggctggc gaaaaaactt atttagtcat
     8221 ggtcgggaaa actggggaag ataagactgg catcatcagt ggggatgtgt tgaatgtaga
     8281 cttagaatct cctacctatt tcgatttcca aaacaaacgg atcggaagcg agactaatgg
     8341 cataggtggc tgggtatttc aaggaaatgg cgattcttct ttgaagatcg ctcattcaga
     8401 aattgcttta tggcgaaagg caggagtaac gttcgataac tttgaaaaag atccggatta
     8461 tttttctgat atgacagact tatcgatcaa tggaaccgac ttaggtaatt ttgtatcaag
     8521 ccctagtgca ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa
     8581 tcgaatcagt gccaataacc agcttccgat gattgaatcc ttaagagtac caacgaatgc
     8641 agataaaaaa atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga
     8701 tgcttggaca ggcgaagtag aagtgacttt acgtattact tatgcagata cgacgaaatc
     8761 acctgtagaa attactgctc tcacacaagg acaaacagat gaaaatgaag ggacaggata
     8821 taatccgtgg ggagaaggcg aacaaggcgg tttgtttgag gttcaagtac ccaacgaaga
     8881 aagactaaca acgggggatc aagtgcaaat agttgctgct aaaaaagtaa gaggcggcca
     8941 agaagtgaca gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgagcctgc
     9001 taaattaagt accacattga tcaatgcagc ttctcgctca atcacaggta caggcgaagc
     9061 aggagctttg gtttcattga aaaaaaatca agctgttttg tccgaaacaa ctattgatga
     9121 tacaggtaaa tttgttcttt ctatcc
//
LOCUS       DASJON010000116         9119 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJON010000116 DASJON010000000
VERSION     DASJON010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.117"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1803)
                     /gene="glmS"
                     /locus_tag="U3989_001976"
     CDS             complement(<1..1803)
                     /gene="glmS"
                     /locus_tag="U3989_001976"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN1715421.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2212..2502
                     /locus_tag="U3989_001977"
     CDS             2212..2502
                     /locus_tag="U3989_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715422.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2515..3540
                     /gene="sppA"
                     /locus_tag="U3989_001978"
     CDS             2515..3540
                     /gene="sppA"
                     /locus_tag="U3989_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1715423.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3543..4202
                     /locus_tag="U3989_001979"
     CDS             3543..4202
                     /locus_tag="U3989_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1715424.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4359..5291
                     /locus_tag="U3989_001980"
     CDS             4359..5291
                     /locus_tag="U3989_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715425.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5445..6734
                     /locus_tag="U3989_001981"
     CDS             5445..6734
                     /locus_tag="U3989_001981"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN1715426.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6981..8231
                     /gene="clpX"
                     /locus_tag="U3989_001982"
     CDS             6981..8231
                     /gene="clpX"
                     /locus_tag="U3989_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN1715427.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8314..8901
                     /locus_tag="U3989_001983"
     CDS             8314..8901
                     /locus_tag="U3989_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN1715428.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8975..>9119)
                     /locus_tag="U3989_001984"
     CDS             complement(8975..>9119)
                     /locus_tag="U3989_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715429.1"
                     /translation="FERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEKKKDK
                     KQK"
BASE COUNT         3014 a         1589 c         1926 g         2590 t
ORIGIN      
        1 ttctactgtc actgatttcg ctaaattacg tggtttgtca acgtcgaatc cccgtaatag
       61 cgttgcgtaa tatgcaagta attgagtcgg tacgactgtg gttagagtag ttagataagg
      121 gtggacagtt ggtagcacca gctggtcact ttccttttct aatccctctg agacaataac
      181 tagtgtttta gcgccacggc tttcgacttc tttcaggttt cctcgagtat gcgcaccagt
      241 cacttcttca ctgatgattc cgatcactgg tgttccttct tcgatcaaag caatcgttcc
      301 atgtttcaat tcgccagcgg caaaaccttc tgcctggata taactgattt ctttcaattt
      361 caaagcagct tcaagagaaa cgttgtagtc agcaccacgt ccaatgtaaa aggcatttct
      421 tgtatcgctg agatatactt ctgccaactc ttctaaataa tctttttggg cgatgaccga
      481 ttccatcgca ttagcaacta aactcaattc atgagtgata tcgaatacaa gagaagtttc
      541 aaattctttt ttatcaccga tagctttggc taataaagtt agtacagcta tttgtgcagt
      601 ataagctttc gttgatgcta ctgcgatttc gggtccagca tgcaatagca gtgtaaagga
      661 tgcttcccgc gaaagagtag aaccagcaac atttgtaatc gtaagtgaag ggtagccgag
      721 acggttgatt tttaccagca cttggcggct gtctgctgtt tcgccacttt gactcaagaa
      781 aataaagaat ggtttttttg aaagaagagg tgtgttatag ccaaattcac tggctaaatg
      841 aacctcaaca ggaatctgtg tcaatttttc caaagtctgt ttacctgcta accccgcatg
      901 gtaactagtt ccgcaagcaa cgatatagat tcgatcgcta tcagacaatg tatcgaccag
      961 cttttgatca agagagacac gattgtcttc tgttacgtat ttttgagcga tacgacgcat
     1021 gataataggc tgttcgtcga tttctttcag catataaaaa ggataggtac ctttctcgat
     1081 atctgaagca tctacttgtg cttcgaatga agaacgttct acgatgtcac ctgcaaaatt
     1141 ttgaataata actttttctg atgtgacggt aaccatctct tcatcttcaa tctcaacgaa
     1201 ttccttcgta tgagcaagca tagccattgc atcgctggca atcacattga atccatcgcc
     1261 gagtccgatt aatagtggac ttttattttt agcgacgtaa ataacatcag gtgctgtccg
     1321 atcaatcaaa gcgaaagcgt aagaaccttt gatgacccgc aaagctttta aaaacgcctc
     1381 ttgcgttgta gtatccttcg cgaaagtttc aattagatga gcgataatct cagtatcggt
     1441 ctcaccaata aagtggtcat ttgctaaata agcaatttta agttcttcaa agttttcgat
     1501 tacgccgttg tgaacgagca cgaagcgtcc actttgggaa gtatgtggat gggcgttttc
     1561 ttcagaaggt tgcccgtgag ttgcccatcg tgtatgacca atacctatat ttcctgtagt
     1621 atcaatattt attttgtttt tcaaattttg aattctgcct tgtgctttga ccagataatc
     1681 agttgtgcca tctgagacaa agataccagc tgaatcataa ccacggtatt ctaatttttc
     1741 tagaccgtca atcaatcctg gtgttacatt ttttaatcca atcattccaa caattccgca
     1801 cataaaaaac atcctacttt ctataatgaa ttcgtatgaa tgcttccagc tgcaggaaaa
     1861 tccttttgtt ttatctttgc agacattttt tgtggatttt ctgtagagtg cagtccctag
     1921 ggccatccgc cgaatctttc gataaaccct ctcctcgtca tctgtttaca cagacctggc
     1981 gctatcctaa aaggtataac tatcctcctt tttgtttttt atctggaaac atttaaaatc
     2041 ataacctttt gcgaatgttc ttgtcaacaa aaaaagaata aaaaataaat tggtatatat
     2101 caaattcttt gtcaaatgtc ctttttatgt caaaaaataa tgggttttta tctatcatct
     2161 ttttcaggag ttcggtacaa tagaaagtaa tcggatagag gaggagtaaa aatggattca
     2221 aaaattgaga tcatgacact gggaatgtta aaaaaacaat tgtccaaatt cgaagcatca
     2281 gctggtgtat cagatgatac gaaaatattt ttagatactg gctgggatag catccaagaa
     2341 atcgcacctg atgccttgga agttgctcaa gctcgtgagt ttacggtaga agatgaatta
     2401 acaaaagagt cgttttccgg gtatgcgcgg gaagaaaaag cagagcggtt cgacacttct
     2461 gagcagtcag aaactgtgat cgtcataaaa aacttgtatt agaaaggaga aagaatgaat
     2521 aaaagacgtt gggcagcagt tctgattgct gcaggattat ttgtggtttc gttagtttca
     2581 gctagtttga ctaagcctca ggaaaacgat caaattgagg gattgaacag ttggttatat
     2641 ggggatgaag aactagctcc aaatgtgttg gaagagggag acagtacgca aaaaatcgta
     2701 aaattatcag tagatggaac gattgctgcg ggagggtctt caggtttatt tgcttctgaa
     2761 ggttacaatc ataaaaattt tatggaacaa ctgaaggcga ttgaagaaga tccaacagtc
     2821 aaaggtatct ttttagaagt aaattctcct ggtggcggtg tctacgaaag tgcggaaatc
     2881 gctaagaaat tggatacgat tcgtaaagaa catgatatcc caatgtatgt cagcatgaaa
     2941 aacatggcag ctagcggtgg ctactatatt tctgctcaag cagataaaat ctttgcaaca
     3001 gaagaaacag tgaccggctc aattggtgtg atcatgtcag gattgaatta ttcaggactt
     3061 ctagaaaaac tgggtgtgga agatacaaca gttaaaagcg gagcgttgaa agatatgggt
     3121 tcaggaacgc gaccagagac aaaagaagaa gaagcggtgc ttcaagcata tatcgacagc
     3181 gcatatagtc gttttgtagc aatcgtcagc gagggacgga ataaatctga agaagaagtc
     3241 aaaaaaattg ctgatggaag gatttatgat ggtgtccaag caaaagaagt aggtttagtc
     3301 gatgaattgg gatttcctga agatgcttta aaagctatac gaaaggaaca acagcttgaa
     3361 gatgcggaac tagtagaata ttcttccacg ccatctgggt ttgctagtac atggttagga
     3421 agtaaactag cagaattcca gggattgaag ccttctgaga ctagtcagat cttatccgtt
     3481 cttgagaatc tagggacagc ggaatcacca aaagcaatgt attattacgg aggtgagtaa
     3541 gcatgtcaga gctttcaaat gaaaataagg aacaagaaaa agataagcaa cagcagcctg
     3601 ctgtaggacc tgactccaac ttttcccaaa aggtactgag ctcttttgaa agagagcctt
     3661 tgacgcccga agaaatcaaa aagaaacaac gtaaatggaa aaagtatacg gacacaagaa
     3721 atgaaccgcc aaaagcaaat gattttccta agtatttcta tgcaggtttt tttatccgaa
     3781 tgtttgcctt cttggtcgat cttttgtgta tcggagctat tacgcgttta acacttggta
     3841 ttgccaaaaa tctaggttgg cttaattttt cagattctta tctgagtctc tacggattca
     3901 cagccttact tatttatcta gcgtatttta ttttattgac gaaattcaat catggccaaa
     3961 cgattgggaa aatgatcttt ggtattcgag tcatcagctt tgatgaagaa gaacttagct
     4021 ggtcaaccgt tttagttaga gaaggtgctt gccggttcat tctaaaattt cctttgctga
     4081 tgttagggta tcttccagca gtctttaatc agaaaaagca acatctgggc gattttttca
     4141 ctaatacaag tgttgttacg atcaatctga tcaaagcgtt caaccaggaa gtcaacagct
     4201 gatgagcaat aaaaagcagt aacagttgaa agcaccttct aaaagtcaga tctaaaatct
     4261 agcttttaga gggtgcttca gttattttta attgtcatgc tttttttgtt ttgctactat
     4321 tgaacatgag ctgaatatga gcaagaggag gacactgcgt gaagccgatt gattttccag
     4381 aaaaatatga gaatttaatg cgagtggcac aacaagcact agcaaaccag caatatcagc
     4441 aggcaaaaga attatttcaa cgagcttacg agttgaaaga aagctttgag gctaatagcc
     4501 tgcttgtttt ttgtctttac gagttggacg agaaaaaaga agcattgaag caagcactct
     4561 tgcatgaaaa acagtatctt caaaatgagg agtttgctga attttatttt gacttgttaa
     4621 tcagtacaca agatttttta tatgctagaa aattgatagc aagtacagat ttttatgaag
     4681 ctttcgagca gcgggtcatt gaaaaaatcc aatttgcaga agaattatcc ggacaaatgg
     4741 agaggcagaa gatcaaggca ctccacaata aatcggagga acttccttcg ttggaacctg
     4801 cacgtcagct tgcgtctatt gatgagatcg aacagcttcc ttatcatgag tttatccaaa
     4861 cagcgagtaa acttattgta tcgccagagg tccatatttt agcaagggca aaattactgg
     4921 agacacttag gcaattgaac gaatgccatc aagttttcta tttgacgatt gaagaaaagt
     4981 tagtcaaagt cataccaaaa gatctgccaa agccgcagca gcaggcaagt taccagcaat
     5041 tatgtgtatt ttcagatcgt tacgggaatg aagatgcgct gcttagttcc gtgttaaaag
     5101 aagaattcac gttgcaatct gcaattgttt atcctgtcta tgatacatac atcgaagacc
     5161 cgaagcgctg gtttcaactg actgtagagg cgtgtacagg aaaaacgatg tatgaaggta
     5221 cagaagatga aaaacaagat tttttaaaaa aaagagaaaa aattttgcag caaatgatct
     5281 tcttccatta gattttatca gtgaaaaaga taaaaatgtt atgaaaaaca aggattctat
     5341 ccattcaata tgtttacaat ttgaaagggt tagtgtacta tttaaaggta tgcatgaaac
     5401 gtttttgatg tataaaatga atttctaaac tcggagggaa ttaaatgact gcaacatttg
     5461 aaaaaaaagg cacgaatgat ggtgttttaa aatttacgat tgcgcaagac gaaatccaaa
     5521 aaggattaac aactgcgttt aataaagtga aaaaaagctt gaacgtacct ggattccgta
     5581 aagggaaagt ttctcgtcaa gtattcaacc gtatgtacgg tgaagaagct ttatatgaag
     5641 atgcattgaa tgctgtatta ccagaagctt atgaagctgc tgtcaaagaa gctggcatcg
     5701 atcctgtttc tcaaccaaaa atcgatgttg aaagcatgga aaaaggtcaa gactgggtaa
     5761 tcactgctga agtaacagtg aaaccagaag taaaattagg acaatataaa gatctagttg
     5821 ttgaaaaaca agaccgtgaa gtaacagatg ctgacgtgga tgcacgtatc aaacgtgaac
     5881 aagaagcaca agctgaatta gttatcaaag aagatgctgc agctgaaaat ggcgatacag
     5941 tagtaatcga cttcgaaggc tttgtagatg gcgaagcgtt tgatggcgga aaaggcgaaa
     6001 attactcact tgaattaggt tcaggctcat tcattccagg ttttgaagat caattagttg
     6061 gtcataaatc aggtgaaagt ctagatgtaa atgtaacttt cccagaagat tatcaagcag
     6121 aagatcttgc tggaaaagaa gcagtcttca aagtgacaat ccatgaagta aaagcaaaag
     6181 aagtgcctga attagatgac gaattcgcaa aagatgttga tgacgaagta tcaacattag
     6241 acgaattgaa agaaaaatat cgcaaagaat tagtagaaac aaaagaaaaa gcagcggacg
     6301 atgcaaaaga tgaagcagcg atccgtatgg ctgttgaaaa cgcagaaatc gttgaattgc
     6361 cacacgtgat ggttcatgat gaagtacatc gttcaatgga cgaattcttg aacaacatgc
     6421 aacgtcaagg tatctctcct gaaatgtact atcaattaac tggttctaca gaagaagact
     6481 tgcataaaca attcgaaggt gaagcagaaa tgcgcacaag aacaaatctt gtgatcgaag
     6541 caattgcagc tgctgaaaat cttcaagcaa cagaagaaga aatcaatgca gaaatccaag
     6601 aattatcaga aacatacaac atgccaatcg aacaaatcaa acgcgtgttg acagaagata
     6661 tgctggaaca tgatgtgaca atgaaaaaag caatcgaatt gatcacaggt acagcgacag
     6721 aagcgaaaga atagaaatca agtaataaat catttctgaa gaagctactt ctagaaaatt
     6781 gtttattcga aataagggac tgtgacacga ttttgtcaca gttccttatt ttttgttaag
     6841 agtaaaaaat gcaaaggtat aggtcagttg atcctttgtt aaacactttt tctttttact
     6901 aaactatgtt atgatagtaa aagactgtaa agtctttgat agtttcttgc atactttttg
     6961 caagttaagg ggtgaagacc atgtatgaca atccaagtag caatgagacc gtacgctgtt
     7021 ctttttgcgg gaaaacccaa gaagaagtga aaaagatcgt agctggtcca ggggtctaca
     7081 tctgtaatga atgtatcgac ctatgtaaag aaatcattga tgaagaattt tatgaagaag
     7141 ctgtacgcga attcactgaa gtccctaaac cactagaaat tcttgaagta ttgaataact
     7201 acgtgattgg acaagatcgc gcaaaacgct cgttagctgt tgccgtatac aatcactata
     7261 aacgtgtcaa tcagcaagca caagaaaaca cagatgatgt tgaactgcaa aaaagcaata
     7321 tctgcttgat cggacctaca gggtctggta aaacattttt agcacaaaca ttagcaaaaa
     7381 cattgaatgt accttttgca atcgcagatg cgacaagttt gacagaagct ggttatgtgg
     7441 gggaagatgt agaaaacatt ttgcttaaat tgcttcaatc tgctgattac aatgtggaac
     7501 ttgcagaaaa agggatcatc tatattgatg agatcgataa aattgcgcgt aaaagcgaaa
     7561 atgtatctat tactcgtgac gtttccggcg aaggtgtcca acaagccttg ctgaaaattc
     7621 tagaaggaac tgttgcaagt gtgccacctc agggcggacg taaacatcca catcaagaat
     7681 ttattcagat tgatacgacg aacatattgt tcatcgtagg tggtgcattt gatggcatcg
     7741 aaacgattgt gaagaatcgt atgggcgaaa agacgattgg atttggtaca caaaaccaaa
     7801 aactttctga agacgaaagt gtcatgcagc atatcattcc tgaagattta ctaaaattcg
     7861 gattgatccc tgagtttatc ggacgattac ctgttatggc agctttagag aaattgacaa
     7921 cagatgatct ggtacggatc ttgactgagc cgaaaaatgc gctagtcaaa caatatcaaa
     7981 aacttctttc tctagataat accgaacttg aattcgaacc tgaagcgttg agagccattg
     8041 cgaaaaaagc aatcgaacgc aataccggtg ctcgaggact tcgttccatc attgaagaaa
     8101 tcatgatgga tgtgatgttt gatatccctt caaatgaaaa catcgaaaaa gtgattatca
     8161 caaaagaagc agctgaattg tctggtgagc caacagtaat ctataaagat tcagagaaaa
     8221 aagcgggttg atgaaaagag gctgccgtaa aagtcatttt ggcttttgcg acagccgctt
     8281 gctttattca tcaccttgtt aaggagagag accatgaaag tccatcaagc tgaaattatc
     8341 ataagtgcag tggcaccaaa acaatatcca gatacacaat tgccagaaat agctctggca
     8401 ggacgatcaa atgtagggaa atcttctttt atcaacacat tgattgatcg aaagaattta
     8461 gcccgtactt ccggtaagcc aggaaagacc cagacattga atttttattt gatcgagaac
     8521 gcattacatt ttgtcgatgt tccgggatat gggtatgcaa aagtatccaa aactgagcga
     8581 gcaaaatggg gaaagatgat cgagacatat ttaacgacta gagaccagct acgtgcggtc
     8641 gtttctttag tagatttgcg tcataagcct tctcaagaag atatccagat gtatcaattt
     8701 ttgaagtatt atgatatccc tgtaattgtt gttgccacta aggcagataa gatccctcgt
     8761 ggtaaatgga acaagcatga gagcgaaatc aaaaaagcgt tggattttga tcgcaatgac
     8821 acgtttattc tcttttcttc cgttacaaaa cagggaaaag aagaagcgtg ggcagcaatc
     8881 gaacaagcaa ttgaatcata aaaagaataa aaaagactta ccagtcaaac tctttcataa
     8941 aagtttggct ggtaagtctt tttttgagct taacttactt ttgtttcttg tcttttttct
     9001 tttcattttg tggctgtggt tttagaaatc gatcgatctc ttcttttggt gcaactgttt
     9061 tttccggatc gacagcaaat gattcgaaaa gtttatctaa attatctgta cgttcaaat
//
LOCUS       DASJON010000117         9115 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJON010000117 DASJON010000000
VERSION     DASJON010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.118"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..80
                     /locus_tag="U3989_001985"
     tRNA            9..80
                     /locus_tag="U3989_001985"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Glu,seq:ttc)
     regulatory      269..348
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            346..1539
                     /locus_tag="U3989_001986"
     CDS             346..1539
                     /locus_tag="U3989_001986"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN1715430.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1669..3141
                     /locus_tag="U3989_001987"
     CDS             1669..3141
                     /locus_tag="U3989_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1715431.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3224..4012
                     /locus_tag="U3989_001988"
     CDS             3224..4012
                     /locus_tag="U3989_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN1715432.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4079..4747)
                     /locus_tag="U3989_001989"
     CDS             complement(4079..4747)
                     /locus_tag="U3989_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN1715433.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4862..6574
                     /locus_tag="U3989_001990"
     CDS             4862..6574
                     /locus_tag="U3989_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715434.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6577..8349
                     /locus_tag="U3989_001991"
     CDS             6577..8349
                     /locus_tag="U3989_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715435.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8431..>9115)
                     /locus_tag="U3989_001992"
     CDS             complement(8431..>9115)
                     /locus_tag="U3989_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1715436.1"
                     /translation="RRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASK
                     QKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKN
                     TGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSK
                     ELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQL
                     VEKLGKIEI"
BASE COUNT         2776 a         1507 c         1949 g         2883 t
ORIGIN      
        1 attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg
       61 ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt tttatctgaa
      121 agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct aagctttttt
      181 ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa aaaatacgag
      241 cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag gatccgcagg
      301 acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt agaaagacac
      361 ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga ccaaatcagt
      421 gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg
      481 tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc atatgtagat
      541 attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc aaaattcgga
      601 tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc tgatattgct
      661 caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt attagatgag
      721 attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac accagaattg
      781 atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa
      841 tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt tgaatatgat
      901 gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca tgatgatgca
      961 gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga agttattcca
     1021 gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg atttgtcatt
     1081 ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga tacgtatggc
     1141 ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa agttgaccgt
     1201 tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg gcttgctcgc
     1261 aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc
     1321 aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt gagagaaaac
     1381 ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa
     1441 caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg ggaacatacg
     1501 gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag ctggaacgat
     1561 cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa aaagatagta
     1621 attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat gaaaaaagaa
     1681 acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac ggctattgaa
     1741 ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg aatggaaatc
     1801 atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc gatttacgga
     1861 aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt attgtttatt
     1921 atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc tgctcgtgcg
     1981 attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat cggcgatctt
     2041 tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg ggggattgcc
     2101 agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg gcactggatc
     2161 ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct atatttaaat
     2221 gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt tagtttgatg
     2281 gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg aatgagtccg
     2341 cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt ttttgccgaa
     2401 aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact atttgttgtg
     2461 gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt ttatatccct
     2521 atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt attagcacca
     2581 ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac tcaaatgaaa
     2641 actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc ttggttgtat
     2701 cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt catcggtata
     2761 ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt atctcgtgac
     2821 cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca aacagtcatg
     2881 gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc agcagcaaat
     2941 ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc taaatatatt
     3001 gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca ttccgtttat
     3061 ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa
     3121 agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat cattcaaatt
     3181 taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat ttattcaagc
     3241 aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac taccgcaaac
     3301 aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac ctgttttata
     3361 tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt cgattgaacg
     3421 ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg gctggtacac
     3481 agatacagtt tatgacatga agtattggac attcattgca gaagagctac ctgagatttg
     3541 tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg ctggattatc
     3601 gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt
     3661 aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt tgatggttcg
     3721 taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag gttcaaaaaa
     3781 tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc caagatttta
     3841 tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg tatctgctat
     3901 gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata actggatctt
     3961 ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag
     4021 tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag gagctttttt
     4081 actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag gtttgtgcac
     4141 tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt tctttaaatc
     4201 cctctaattg attttcaact acataattac gcataaatag aactgtttct ttcaaaaatt
     4261 catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc attaactgtc
     4321 caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact gaaaatcctt
     4381 cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga aaagcgggca
     4441 cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca tagtaatttt
     4501 ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc gctaattctt
     4561 cttccattgt ttcagccatt atttgcaaga gctgttgttt gtttttgaaa taccaatata
     4621 aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc tgcgaaagag
     4681 cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga gaaagcttta
     4741 tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa attgttagtc
     4801 ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt gtaatcaacg
     4861 aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta tttccttgct
     4921 gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac agcaagtatt
     4981 ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt acctgatcag
     5041 tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg caaaagtagc
     5101 tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga cattttcatt
     5161 tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac
     5221 gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc cattcttatt
     5281 tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc
     5341 gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg gcaagcactt
     5401 catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg
     5461 gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt
     5521 aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg tgatgatccc
     5581 gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg tgagcgatct
     5641 tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact acttgatgca
     5701 gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa gagcagccgt
     5761 atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt atttagacgt
     5821 tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc gttatcctgg
     5881 tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag aaatggttgg
     5941 tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc ctcgtctatt
     6001 tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa
     6061 gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct caggaacgat
     6121 tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc
     6181 ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg aagcaccaat
     6241 agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg
     6301 cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac tggatgctcg
     6361 ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa cgacgattgt
     6421 catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat tggatgaagg
     6481 cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg tctatcagga
     6541 aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat aaaagcaagt
     6601 aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat ctttatcaca
     6661 gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga agcttttcaa
     6721 gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag caaatttgtt
     6781 tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa ctttatctat
     6841 agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat cggtctattc
     6901 aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg tgaaatttta
     6961 agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc tttgcttcaa
     7021 gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg ccaaaatgta
     7081 gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc tgtcttgatt
     7141 atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa actaaatggt
     7201 tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt acaagaagaa
     7261 acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt caaaggacaa
     7321 gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa tacagcgata
     7381 gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc ggtggcatta
     7441 ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact gatgcagatt
     7501 tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt gaatgaaata
     7561 tttgatgaaa aagatgaagt caaaccagaa aactgtaaag catttgatgg tgttgaaaaa
     7621 ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt cttgaaagat
     7681 gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac aggatcgggg
     7741 aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc
     7801 atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca tgtaggaatc
     7861 gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac attcggcaaa
     7921 ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat tcatgacttc
     7981 atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa cttattcagt
     8041 acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc atctttattg
     8101 attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat ccaaaaagca
     8161 atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct gaaaacaatc
     8221 ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga aggcagtcat
     8281 cacgagttat tagcagaaaa tggattttat gcggaacttt atcataatca atttgtattt
     8341 gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt ttatgttgtt
     8401 tgtttttata aggggcagaa aaggggcaat ttaaatttct atttttccta gtttttctac
     8461 tagttgattt tgcatatttt tagttgtatg agtataaatc tttaatgttg tttttgaatc
     8521 ttcatgtcct acacgatcca taatggcttt taatgggatt cctaattctg taagtagaga
     8581 aatgtgggaa tgccttaaca tgtgagaaga aactttttta tctaggttta attctttgga
     8641 tactgctctt aaagattgat tatatgcttg aatagatatc gggttaccat ttttagagat
     8701 gaaaatgtat ttatctttat tattcttaat taatttgttt tcttcaatta attgatctat
     8761 gatttcttta gcattacttg ataattcaat ggttctataa gaacctgtat ttttagttgt
     8821 tgttttaatt tttaactcgt aatcataagt accagaaacc gtaattttat taccatcata
     8881 atcagattca gtcaaagata tcaattcacc ataacgtagt ccagttaaaa acaaaaattt
     8941 agtaattaaa gaataacgtg actgttgatg tgaacttttt tgtttactag caatcatttg
     9001 aacttcttct tttgataaat aaccatcttt ttcttttttt atttcttcgt ttgtcttagg
     9061 ttttcttgct aaagaaaccc catttacggg attatatttt aatccatatc gacgg
//
LOCUS       DASJON010000118         9048 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.119, whole genome shotgun sequence.
ACCESSION   DASJON010000118 DASJON010000000
VERSION     DASJON010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.119"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(78..1079)
                     /locus_tag="U3989_001993"
     CDS             complement(78..1079)
                     /locus_tag="U3989_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1715437.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1084..2997)
                     /locus_tag="U3989_001994"
     CDS             complement(1084..2997)
                     /locus_tag="U3989_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1715438.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3165..3671)
                     /locus_tag="U3989_001995"
     CDS             complement(3165..3671)
                     /locus_tag="U3989_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1715439.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3831..4271
                     /locus_tag="U3989_001996"
     CDS             3831..4271
                     /locus_tag="U3989_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1715440.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4297..5454)
                     /locus_tag="U3989_001997"
     CDS             complement(4297..5454)
                     /locus_tag="U3989_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715441.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5457..5813)
                     /locus_tag="U3989_001998"
     CDS             complement(5457..5813)
                     /locus_tag="U3989_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715442.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6111..7085)
                     /gene="sgrA"
                     /locus_tag="U3989_001999"
     CDS             complement(6111..7085)
                     /gene="sgrA"
                     /locus_tag="U3989_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN1715443.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7281..8120)
                     /locus_tag="U3989_002000"
     CDS             complement(7281..8120)
                     /locus_tag="U3989_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1715444.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8305..>9048
                     /locus_tag="U3989_002001"
     CDS             8305..>9048
                     /locus_tag="U3989_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1715445.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNK"
BASE COUNT         2706 a         1829 c         1367 g         3146 t
ORIGIN      
        1 aaagcttgtt tattgaaatt gctccttgtg acttggagct aagtgacaga ttacaacctc
       61 ttttgcatca taagacttta ctccttgaaa aacattacta acttttgata tgtttcttgc
      121 gcttgtttat tgctgaaatc aaactgatat tcatggttca aaacagcatt tttctccgca
      181 taatctactt ttgtgacttt cacccccgct ttctcaaata cagaagttac ttcttccatt
      241 tgtttcgtaa acgtatttgt atttccatcc gttaagaatg tgttttgcgt ccaacgatcc
      301 gcatgtctga ccaaggctaa acttttgacc ttctctgatt gttcaaagtc cttattatca
      361 aagtaagccc atccacattt ttcataaaga aagctagaga ttttatcact tacttgagta
      421 agtgcagaag gatccaacag accagacaaa gaaataaact tcgtaatctg cttgttctcc
      481 aaagtcggat caagatgatc agctttttta atttcttcat tttgaagtgc taacaaatat
      541 tctccactaa tttgtgcacc tgccgaattt ccaccaatcg aaatattcga ccaatctgct
      601 tgatctttta tctctttttt agctgcttga atggcttcgt tcaattggat aactggtgta
      661 ggatgttttg cttccggtga tcgttcataa tctatattta cgatgatttg ctgcgtgtct
      721 gaacatagat attccaaata atcacgacaa tcatttttat cccctcctac atatgcgcct
      781 ccatgcatcc agaaaagaat cggtaatttt ttcttttcca ctttcggtgt gtagatatcc
      841 atattattat ttttgtactt tgaaggatag gatatttctt tttttatatc gatttgttcg
      901 attttttgct ggaaatcatc tggtcgggca tatacttgtt ctttttcact tgtgtcaaat
      961 tgttttctaa caagcaagct aatcggcttt ggtgtgattt gattaatcaa aaatacagtg
     1021 actaaaataa ctacgatgat aactggtact gcaatcacta ttcgcttgaa ccatttcatt
     1081 catttaccct ctctttcttc gccaaatcca tgtgataatc gtgctaacag ataaaaatcc
     1141 acctgaataa atgagaagcg aatgtttctt ttctccagtt ttcggcaaca ttcttttttt
     1201 tgcatctgtc tttttttgta ctttcactgt catctgaatc ggttgcgtat catcacttga
     1261 ttgatcctct cgaggttcat tctttccctt attgtctgca ttctctgtcg gatcttgctt
     1321 ttttggtgga gctactggga tttttttgta atagaaaata atagttggta tcgtctcatt
     1381 aaaaatccct tcagtcggtc cttcaacatt tgctaattcg tatccttcga atgtcttttg
     1441 ctctgtcata taattttctc cataagctcc gtaaagctgg atttcgtcct ggtctttgag
     1501 tagactttta ttgttttcat ccacatactt tacgtttatc tgatcatgga acactacttt
     1561 caaaaagtcc gttacatgtt tcgtgtaggc agcatggtca acttcctgtg cctttccatg
     1621 ccctgctcct tctactaata aaagatcttt ataccctcct gataactcat ataatttttg
     1681 cgcgttagac acaggtacta catcatctgc tgttccatgg ataatcaatt taggcaatga
     1741 atctgacatt ccaatcgtag gtagagcagc ttttgtatcc aaatgcatct cctgatcaaa
     1801 atagtattgt ttcaaaaggc ccataaataa atcttcatgc tcttgatccc caatgatgtc
     1861 taactgattt tttatggcat ccggtagtgc gggggctact gtctggctgt acgtaaaacg
     1921 tagttgttca tcaattgatg caaatccgca attttcaata attcctttga cattgacagg
     1981 aatatctttc gctaatacag acatcatcgt agctgctccc atcgaaccac cataaagtaa
     2041 aatcttttga gatggccgct ctcgaacttc ttgatttatc cagtaaagga cgtcatctga
     2101 ctcatattgt ccataggtca cataatcgcc tccgctatta ccagtcgctc tggaatcaac
     2161 aaataataaa ttatacccgg ccttataaaa atcattgtat tcttctgtaa cgatcccgtt
     2221 attccaattc cctcgaaatc cgccatggat catgactgtt ttatctgaac cttgatcgac
     2281 ataatatgca tatacatcca tcattttgcc atcttgtttg tttagtacag tttgatgaag
     2341 ttgttttgta tttggataga agcctgtgtt ttctgtaggc gttgtcccat catctgtacc
     2401 tccaagcatc gacatcaaca cactgttttt tgcaaaagcg atatcgtaca atagataagt
     2461 agcaatcaga tgtttgataa tctctttatc tacaccataa tcattaacag caagctcagt
     2521 gactaataac tctattaatt ttgacttgat gtcttctgtt ggtccattaa gtatttctgt
     2581 catctgttta ccgaagtaat tcaaatattc tttaccattg aaattttcac ctttaccgat
     2641 atcatcattt tctgtcccat tctctttgtc acttacttta gcagaagagg attcgtcgtt
     2701 tgacattttt gtagctgttg cttcttctgt ttgttggtta tctgtggtag cggtcgtttc
     2761 ttgatctgtg ctagtaacag atgtctcaac cgttgtttca gtatttgttt ctgtttgtga
     2821 agtactcgat ttcgtttcat ttccagcatc agtagtttct gtggtttcta cctcccgaga
     2881 ttccgattcg gaagaaatgc tctctccata tactgtccat tgcgggacgc ttgtacttac
     2941 agcaaataag atgaccaacg ctccctttaa tacttgtttt gttctcaagc tattcactcc
     3001 tttttatgtt tctaggagaa ttatatgtaa acgcaaacat tttttataca aaaaataaat
     3061 ggtttatccc ataaaaatag aacaggatta ttttctcatt ttacgtaata aagcagaatg
     3121 gataaaccag tatccattct gcttttttta aacgaagaaa caacttattg ctccaaaaat
     3181 gtcagaaatc gtttatccca aattttctcg taaaaagcga ttgtttcctt tgctttcata
     3241 aaaggattat cataagttgt cgttgctcct ctacacatca taagctggta tcctaatcca
     3301 tgagcaaact tcacagtcgt atctatacaa tactctgctt gtgccccaca taattcgatc
     3361 gaatgacagt ccaattcagt taataaaaca ctcaaattag tcttataaaa tgaattcgca
     3421 tgggtttttc tcacaaaata actgtgctca ggaatagata attccgacaa tagctcccat
     3481 ggttttgttt gtggcactaa ttgagcatct tcgtgttgaa cgaaaataac tggtttccca
     3541 atcgtttcgt atgttttgat tcttttattc actaatgctg tcactgtttc taattcgtgt
     3601 atcggcgaag aatcaaaaca tacccctttt tgtaaatcga tcactactat tgcgtcggct
     3661 gtgtttgaca tacttgtgcc ttctttcttt tttcttgatt taatagtatt tcttatctag
     3721 ctagttccat tatacgtgac aacattttct tattttacac ctctaaatgg cacttttcga
     3781 ctatttcttc atgttattat ttcataaata cttagctatt ggaggaatgt atgatgatta
     3841 aattgtatac aggttctggc tgctcgtctt caagacgcgc aaaacgttgg cttatcgtcc
     3901 atgatatacc gtttatcgaa cagaacttaa ttcgtcaacc actaaccaaa aatgaattta
     3961 tccacttact aaaattgaca aacaacggcg tgaccgaaat gctctctact cattctatta
     4021 gttataaaaa tcttgttcac agaatagatg aactcagttt gagtgaacta tatattgaga
     4081 ttcgtacatc cccttcttta ttgaaaaaac cgattatcta cgaccaatct gcaaaacttt
     4141 tgactggatt tagcgaagat aaaatcagga tgttcctttc taaaactaaa agaaagcaag
     4201 agctcaacga actcttgctt tctggtcatc ttgtcgattt taaagaaaat aatctttttg
     4261 caatgtctta attttggcat aaacaatctg cttttcttaa ttaacgaaat ccaatttttc
     4321 caatatggat ctatttttct cattcctgat ttttatgatt tcagctttca gacgcgccca
     4381 atcatttttt gtaggttgtc cattccagta aaatatctgt gtattcgttt gatcatatgc
     4441 ctttttgtcg ataggataat cgctaatgac aatatcaggt aactgatcag cggatgcact
     4501 atattttatt gggcgttcag tcatagataa tatccttttc tttgcccata attcctgtgt
     4561 tttaccatac tttgattgaa tatagaatat gacaggttgt tcgtgtttta taaaaatatc
     4621 cctgactaac atacagtatt gaaaaatcaa ccgtctgttc ttttcataat aagtattaag
     4681 ctctggaatt ctgcgagcta ccccactaaa aaactgttgc atgattggat acatcactgg
     4741 aaatatatgc tgaaaatctc tgtcttctgc tgattttcca aaagcatcca ccttcttttt
     4801 tgtcccaaaa acccttgaat acaaatggat cattgccaaa ttgacatata gataattttt
     4861 ttctgaagtt gtaaaacaaa tcttgaagtt tttttcaaaa ttttctacaa tagacgattc
     4921 aatccagccc caactttctt gtactttatc tggagtatcc aacaaatctt cttccaaata
     4981 ggtgttcatt acaccaaaca taaaataaag gtaagcttgc tctgttggag tgatgtgatt
     5041 catcgaccag aagtttgctt caaaaaaagg ctctagtatg taatgcgtaa actcttcttt
     5101 tgttatcaat ggatgcacaa atttgttcat ctcttctggg acatacgtaa tcgagtggcc
     5161 ttgtttcatt cgttgcaacg aaattcttag taggctgatc catttctttc tatctatact
     5221 acgcgtatat ggcgcatgtt tcgcaaattt ttctaatagt tgacgtttat tttttatact
     5281 tgataatata tcagaatgat tttcgtcgaa tgactgccaa tacatcaagt gaaaaaagta
     5341 tcggatacgg tattcttctc cttctatcgg attacctttt ttcaagctgc aagataaatg
     5401 atagtttttc aaaatagtat taagttctct taattttctt tgaatcgtac gcaaacttat
     5461 ataatttttt gactaagatt ttctaaggat tgataagtac ctttgtaaag tgaatccagt
     5521 aaaatataac caatcgttcc ctctaaaagt atagaataaa ctttactcag taaaaactga
     5581 gaagaaaact cgacttttat catcttgttt tctgaaagaa aagataattt atattctgga
     5641 tacctttcgc aaaacgattc tgcttgttga atcaccgaat tcatcttagc cagggtgtag
     5701 tttagatcta aagcgatatc cttcaccaca tataatttct cgtctctttc ataacaatag
     5761 ttcaaaaatt gatatacttg cctttcattg atatcaataa attcttcaaa caaagtacat
     5821 actccttttt atctattatt tagacttatt ccgacagcta catgaataac gtacaaatag
     5881 attcgttact gatttttcag agatactacc atgcgcagtg tgcattgtca attattcttg
     5941 ctcttatttt ccatttgtgt tcctgttatt tcctgtttct caaatatgtt agtaaacaaa
     6001 aatagatctg gcaaagctac aacttcgcca ggcaatacag cactctcgaa tgaaatattc
     6061 actcaaaaat ctatcgtttt attcaatttt agatctgttg cttttattct ttatttcact
     6121 cgatggaaga gagaacgaaa atagcttgct cctacaatca aaatgagtaa cacacccatc
     6181 catgtaatta gcttgttcaa ggttctacta ccagtttctg gtaaatcgct ttttctatta
     6241 tctgtttctt tcgctttttc ttgatgatca attccacgag aaacaaaatc aggctgtttt
     6301 agcacacttt ttaggttgct ccctgcattt ctcttttgtt ccttagttgg tatgataggt
     6361 accaaatcaa gttcattatt tacttttcct gtgctgcttt cgctgctcgt gcttcctgtg
     6421 ctactctcac tactcatgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt
     6481 tcgctgctcg tgcttcctgt gctactttca ctactcgtac tctccgtact gctttcgctg
     6541 ctcgtgtttt ctgtgctact ttcactgctc gtactctctg tactgctttc accgctcgtg
     6601 ttttctgtac tgctttcact acttgtgcta tccgtactgc ttggcggtcc aacaactgtc
     6661 caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc catcataaaa aacaatcaaa
     6721 tcaactttat ctttactctt gatctcatca atcgatagtt ctgcagaaat cattattgta
     6781 tactcaatac cagcggtcca ttgtacgtct tttaaataaa cttccccaat aactttttct
     6841 gaacttccat taccatccca accatatgtt agaacagcat gtacatcact agcattcacg
     6901 cttttgtttg gtttgactgg aatagtaatg acttgttgtg tcatgtcata aacaatccga
     6961 tcagttttca cagtaaatga aagattctca tttgcccgtt catttgcaga agtactgatt
     7021 ccaatcattg ctgcaattat actcagccca agaataacaa ataatacggt cgctgttttc
     7081 ttcatttcta tcactctcca tataattacc ttctatttga taaaggaatt atataaaatg
     7141 aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc gtgtattcgc
     7201 ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa gaccgatatc
     7261 agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat ccgctttgtt
     7321 ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt agttgatata
     7381 gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg gatcagagtg
     7441 gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc tccttgtgtc
     7501 caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact gtagataagt
     7561 gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt catgttagga
     7621 ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat taacggacca
     7681 aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga tgcccccact
     7741 gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc tgaatctaac
     7801 cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc aattccaaca
     7861 acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc ataatcttct
     7921 attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac agctaaatgt
     7981 ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat atccgcaagt
     8041 gcattattga caagaattcc taaaacaaaa ccaactaaga ctattaaaac agatccaatc
     8101 aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac aacccttttc
     8161 tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata gatttttacg
     8221 aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta attcttctgt
     8281 tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact actaattatg
     8341 tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa tgaaacgatt
     8401 gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc aaaagttaaa
     8461 tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga tgatgtatac
     8521 ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat caaaaaactc
     8581 ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac atataagaaa
     8641 ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa attaacagat
     8701 aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca gatcattcta
     8761 ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg agaaaatttc
     8821 tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga tggaaaaatg
     8881 aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc atttaaacta
     8941 aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta tcagcaaagc
     9001 tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaa
//
LOCUS       DASJON010000119         8873 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJON010000119 DASJON010000000
VERSION     DASJON010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.120"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="U3989_002002"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="U3989_002002"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1715446.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1708..2025
                     /locus_tag="U3989_002003"
     CDS             1708..2025
                     /locus_tag="U3989_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1715447.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2229..2594)
                     /locus_tag="U3989_002004"
     CDS             complement(2229..2594)
                     /locus_tag="U3989_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715448.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2802..3284
                     /locus_tag="U3989_002005"
     CDS             2802..3284
                     /locus_tag="U3989_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715449.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3481..4371
                     /locus_tag="U3989_002006"
     CDS             3481..4371
                     /locus_tag="U3989_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN1715450.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            4696..7167
                     /locus_tag="U3989_002007"
     CDS             4696..7167
                     /locus_tag="U3989_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1715451.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7270..>8873)
                     /locus_tag="U3989_002008"
     CDS             complement(7270..>8873)
                     /locus_tag="U3989_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN1715452.1"
                     /translation="KAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKIL
                     FDTNNETLNEQRNSRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAIL
                     RIAEPTSGFLPRTESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQ
                     NPNQAEVIMQTPDQWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLID
                     DADSRLLLLNPKMQLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLT
                     EGRQTLDLSLRYFTNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLI
                     GFTETLLDGAKDDPQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEP
                     YFQQIVQNYQPIIEKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEII
                     IRYQATDDLSFSVQDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQ
                     LLNGTITVDSHLGTGSTFTVTIPKM"
BASE COUNT         2916 a         1472 c         1715 g         2770 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat tttttcggta
     1681 tttaatattt aattaggagg gaatattttg cttttttata gtaattttat cttaatagta
     1741 gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaatgc aagtataggt
     1801 tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca aactattttt
     1861 tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt gaatattata
     1921 gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc aattattaca
     1981 caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag
     2041 ttttggtgat agtgatatta aaaatcagaa aagatagaaa aagcaggaga tagataaaat
     2101 gctgatattt cagcattttt taagagtata aaagagaaaa aaagtaataa atgtatacat
     2161 gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg
     2221 gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca taacgaccca
     2281 aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa
     2341 ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta
     2401 gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca
     2461 gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca
     2521 agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag
     2581 caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt agttttattg
     2641 taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa atgaataaac
     2701 tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag atagaaaatt
     2761 gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga aaagcagtaa
     2821 tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct tttacatggg
     2881 taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc ggatttgtgg
     2941 tttctaggaa ttttccagct ttgactgttt tctatttagt gataagtgta ggtctcttat
     3001 taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct tgcttattag
     3061 tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat ggtttgagcg
     3121 agagtggatt gaaggattca gcttattcct ccatgtattt cttgacgatc aaaccatggt
     3181 tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg acagaaatca
     3241 ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg atcatcagta
     3301 aaaaagcata tgagctgcgt aaaatgcatg tttctcatat gcttttttta ttattatgta
     3361 aagaatgcga aaaacgttta catgattttt acataactta actatatttt tacagcaacc
     3421 atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag gagcaataaa
     3481 atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg atgcggctca
     3541 ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc aaatgaacaa
     3601 gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc agcacaagaa
     3661 agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg aagcggaact
     3721 ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt atttgcagaa
     3781 gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt ggtcgggatg
     3841 gcgccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga attgattgat
     3901 atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca aaaaatcaac
     3961 gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg gggactggat
     4021 ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg gcagctggta
     4081 agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga ttccacacaa
     4141 gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa cagctgggga
     4201 atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt ccaaaaattc
     4261 ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt gggctattta
     4321 ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata actagcagat
     4381 aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc cacatataca
     4441 tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga gctaataccg
     4501 agggtcagct gattaaacac cgtttattcg aaaaaaagag ctacgacata gaactgtcgt
     4561 agctcttttt ttgctatatg acaggaatga aaggtgtttg caagcttaat gtgaggaaca
     4621 ctttccatat ctgttctata ctggatagga gaagtttgga aatatccaga aaacgaaatc
     4681 aatcgaggta gccacatgaa gaataaatgg ataaatcgaa gtaaaaaaaa gaaagtaaca
     4741 caaagatcat cagataaagt catccgtctt tttccatcgt ataaagaagg attaacggca
     4801 gatcaagtca aggaacgaat agaaaaaggt gcagcaaata attcagtcga tcccacattc
     4861 aaaacaaatc agcaaatcgt attggaaaac atatttacgt atttcaattt gatctttctt
     4921 atcttagcca ttttgctttg tttagtagaa tcctataaga acttgacttt tttaccagtt
     4981 atcatagcca atacaggaat cgccattttt caggaaattc gatccaaaaa gatcttggac
     5041 gaactaaatg ttttaaatac agcaaaagtc aatgtcatac gggacgggat agaacaaaca
     5101 atagatatag aagaccttgt aatagatgac attgttattt tagagactgg acatcaaatt
     5161 ccagcagatg cagaggtcgt ggaaggaaaa ctgcaagtta atgaggcttt attgacaggc
     5221 gaagcagatg aaataaccaa agaaataggc gatccattaa tgtccggcag tttcattgtt
     5281 tccggaaaag cttacgcccg attagacaaa gttggagcag attcgtatat ttcccagctg
     5341 acagtcaaag ccaagacaat gggagaagga gaacagtcgg agatgattgc ttctcttaat
     5401 caattgatca aatggattgg gatcatcatt attcctatcg gtatcctgct tttttctcaa
     5461 agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg ttgcaatgga agcagcattg
     5521 atcggtatga tacctgaagg actgtacttg ctgacgacga ttgcattagc attaagtgcg
     5581 actaaactag caaagcaaag agttctttta cacaatatga aaagcattga gacattggca
     5641 agggtaaatg tcctttgtgt agataaaaca ggaacgatca cagaaaataa aatgtctgtt
     5701 caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc agtcagatca attggaagca
     5761 tgcattgctg actatgcaaa agcgatggca ggagacaatg caacaatgga agctgttaaa
     5821 gcatatttta caaaaaccac tgatcaatcc tatcaccatg ttattccttt ttcttctgtc
     5881 caaaaattca gcagtgtagc gttggcagac aaggtgtatg ttttaggagc gcccgaaatg
     5941 gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg tttctttcgc tgaaaaaggc
     6001 tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg aagaaccagt attgacagaa
     6061 gcagtcacac cattaggcta tattctgatt gctaatccta tcagaaaaga agcaagagcc
     6121 acatttgatt acttcaaaaa acagcaagta gccatcaaag tgatttctgg agataatccg
     6181 ctcaccgtat ccaatgtagc tgtccaagca gggatttcac atgctgatca gtacgttgat
     6241 gcatctaaat tatcagaaga agaatatgaa gcagctgtcg aaaaatatac agtttttgga
     6301 cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga taaagcgtaa caatacagta
     6361 gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga ctgcagtata
     6421 gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt acttgattct
     6481 gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa caatattgag
     6541 cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc actgttttca
     6601 gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat tagtttgttt
     6661 actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg aatcagaggg
     6721 aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga tatgatcgta
     6781 gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga tgtttctaca
     6841 gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat ctgttcaccg
     6901 ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt tttttcggtc
     6961 atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact tttgctgttg
     7021 agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt ggtagaatat
     7081 ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagc taaaaaattt
     7141 ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt aaaaaagaac
     7201 aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg cacgagcagt
     7261 tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc caagatgact
     7321 gtctacagta atcgtaccat taaggagttg cacgtagtct tttacaatcg ctaaccctaa
     7381 tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg cggtaaaaac gctcaaagat
     7441 ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa acgatagatc
     7501 atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt ggacagcatt
     7561 ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa atggttcgtt
     7621 ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat aattttgaac
     7681 gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat tctcgccatc
     7741 ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat ctttttgcat
     7801 gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca gtaaagtctc
     7861 ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt tgccaacgaa
     7921 atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca cccctagaat
     7981 ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg tctgccgacc
     8041 ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt gatgaatcag
     8101 ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt gatagcttac
     8161 cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat cagcatcatc
     8221 aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct cttctgtggt
     8281 agtacacgcc cgatacatct tactcatttg ttcgctcaac gcattaatcg tctgatacaa
     8341 ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat tttgcaccat
     8401 tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga tcagataata
     8461 gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc aacggcgaaa
     8521 actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa tggcttctaa
     8581 ctggccactt tttttgactg gtaatgcgac atataaaaga tcctcattca aagtagtact
     8641 tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt cgggacggga
     8701 gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc cagtagcatc
     8761 cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag ttttgttttg
     8821 cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt ttt
//
LOCUS       DASJON010000120         8733 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJON010000120 DASJON010000000
VERSION     DASJON010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.121"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(89..1045)
                     /locus_tag="U3989_002009"
     CDS             complement(89..1045)
                     /locus_tag="U3989_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN1715453.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1149..1751
                     /locus_tag="U3989_002010"
     CDS             1149..1751
                     /locus_tag="U3989_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1715454.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1839..3338)
                     /locus_tag="U3989_002011"
     CDS             complement(1839..3338)
                     /locus_tag="U3989_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN1715455.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3354..3878)
                     /locus_tag="U3989_002012"
     CDS             complement(3354..3878)
                     /locus_tag="U3989_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN1715456.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4176..4925
                     /locus_tag="U3989_002013"
     CDS             4176..4925
                     /locus_tag="U3989_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1715457.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4916..5701
                     /locus_tag="U3989_002014"
     CDS             4916..5701
                     /locus_tag="U3989_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN1715458.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5670..6386
                     /locus_tag="U3989_002015"
     CDS             5670..6386
                     /locus_tag="U3989_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1715459.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6488..7522)
                     /locus_tag="U3989_002016"
     CDS             complement(6488..7522)
                     /locus_tag="U3989_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN1715460.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7526..8584)
                     /locus_tag="U3989_002017"
     CDS             complement(7526..8584)
                     /locus_tag="U3989_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN1715461.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQSMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2628 a         1765 c         1558 g         2782 t
ORIGIN      
        1 tgaccgagga gttgacagga gaacaccgtt taaagaagag gttgtgaaat cagctttttg
       61 acctgaatat tagctgaatt cacaacctct atttttggat tttttgttgg gagaattctg
      121 ccttgatttc aataaaactt ttaaaagact gcaaaaactg gaacaattct gctcgatcct
      181 cgaattcagg gcgactagtc ggcagaggca tttgccgata caatacataa acttcttcaa
      241 tacgtgttaa aatttgtctg ccatcatttg actctgaaaa tgtttctgca gtataaatca
      301 ataaaccata gatatgtttt ccataataag ctggttgctg gattctctct agattttcca
      361 gcatgtcttt aatcacattg gcctgtgctc ttctcataga gaagtaagtt tcgtagtata
      421 gcggctgtct gagccagtag ttttcttgat gttctctggc atcttcttgg ctttcgcgaa
      481 tgaatgtcag taaatgttcg catttgatct gcacatcttc tttattttct gataaaagaa
      541 attctgccat ttgtctcaaa atatttcgaa attctttttc gatctgtatt tggttttcct
      601 tcagctgctt cgtacgatct ggcatgtaaa catttgcaag caaagcaaat actaatccaa
      661 tcgccattaa tcccgcttca ttgccgatca gctgccaaga catgttttcc tccactaaat
      721 aatgagtgac caaaaccgaa tttactacta tcccttcggt taatttgaag cgagaggcaa
      781 gcgggatgaa caggaataaa aaaagactga agccaaaaat agtataccca acagcaaaaa
      841 aactgaacag tgctgttgcc gtagctaata taaaggctgc gattcgattc atgcccgtac
      901 gcaatgtaga gcgcttggta tttcctacac ttaataatgc aatgatccct gcagctggcc
      961 aatacaacag cgataagcta cccgctgtga ccatcgaaaa gaagacactg atcactgttt
     1021 tgactgttcg cattcctata cgcatttact tccccctttt ttattttaat tatacttgaa
     1081 taatgaaaaa ctagctatca gacaaaagtt taggtatgat agtaaaaaga ccaaaaagga
     1141 gaatctctat gcaatttggc cgttttcgac taggtatgcg tacgatcaaa agtgccttag
     1201 ctgttttttt atgtatcttg ttttttcatt tgactcaacg aggaatcccg atgattgctg
     1261 cattatctgc tgttttttct ttgcgtcagg acttgactac aaccgtttct tttggaaagt
     1321 ctaggatcat cggcaataca ttaggtggta cattagctat catttatttt tttgtcaaaa
     1381 gctattttca caatgatttt ttggtagaat tgatcttgct tcctattcta gttattattg
     1441 tgatcgtcgt gtcagatggt attaacaata attccggtat catttccgca atagctacgt
     1501 tgttgctgat tgcgttgagc atccctcaag gcgaatccgc tttgtatgct atccaacgtg
     1561 tactagatac ttttataggg acattcattg caatcggtat caatttcttt cttcgtcctc
     1621 ctgaaacaga aaagaaggaa gaactatcag aagatttggt cgaactccaa aagaaagaac
     1681 gattgctaag aacaaattta caagagatag aagctaagat ccaaaaacaa aagaaaaata
     1741 gcgaggacta attttctttt gcaataaaat aaaaaaatgg agagaagaac tttaaaaagt
     1801 cttcctccat ttttgattga acggtgaagt gtactacttt attcttcctt catcgagttt
     1861 tgttctgcct cttttggcgc ttcatacata tttacttggc tagtagtccc acgtgtcgac
     1921 aaattctcta cgatttcctt caagtcaagt cctgttgtcg cttttagtgt ttcttgagta
     1981 gaagaaagca aatttgtggc ataatttgtc actcggttcg ctccactggt ttctcctgaa
     2041 ctgcttcctg tatcgacaac agagattttt tcgatatttc ctagcggctg tgctgcttcg
     2101 cgcatcaatt gcggtagcat atcgataacc atactcaaga cagctgcttc gccatattcc
     2161 ttgaaggcat tggcgatctt ttgttttgct tcggcttctg cttttccttt tgctagtgct
     2221 gcttcggctt ctgcttgtcc agctaaacgt gtcttattgg cttccgcttc tgccagtgct
     2281 tccacacgga atcgttctgc ttcggcttca gtcacttctt tgactttttg agcttgtgct
     2341 tcttgttctt tagcgtaccg atcggcatct gcttttttct tcacttccga atcatattgc
     2401 ttttcacgac gtgtgatttc tttttcttcc agttcgattt gtttttgacg ttctacgatt
     2461 ttaatctgca tttcctgttc gattacttgt tgttgtgctc gggcactttc taaattgtag
     2521 gcttggtctg cttttgcttt agcaacatct tgttcttgtt tgtaggttgc taatttcagt
     2581 tctttttctt ttaatgattc tgcgatctct gtttgtcgct gtaattcagc tgcttgtgat
     2641 tctttttctg cttgagcttt tttgatacgc gtttctttta gagcttccgc ttctgctata
     2701 tctgcatctc ttttgacttg ggcgattctt ggtttcccta aagagtcaag ataaccgttt
     2761 ttgtctctga cctctttgat agtgaaagaa acgatgatca accccatttt tgcaaggtct
     2821 acagaagcga cttcttgtac gctttgactg aacttatcgc gattttgata gatttcttct
     2881 acagtcatag aaccaagaat cgagcgcaag tgtccttcta atacttctct tgcttcattt
     2941 tccagttctt ctcttgtctt gcctaaaaac tgttctgctg ctgtagcaat ctcttctaca
     3001 gatgaaccga ttttgatgat cgaagtacca tcacacatca ccggtacgcc ttgttccgta
     3061 tacacttcag gggtggagac gtccaattta ctcgataata gactgatccg attcgaacgt
     3121 tgaaaaactg gcaagacgaa tgcgccacct ccacgcacaa ttttgatttt gttattgctt
     3181 tcatcaatgt gcacattttt gcttcctaaa taacttccac tgatgatcaa cgcttcatcc
     3241 ggttttgctg tctggtactt cgctacaaag acgatcagca acatcagtaa aatcaaagct
     3301 gcgattgcga tcgcgataaa aacaactggt atttccattt aacacacgct cctttaagct
     3361 tttatcatgc tttcataagg catgacataa cagattcttt ccttgatctc aatgatcaat
     3421 actttcgttc cccgctcagc tgtctgttct tgggggctgt aaaaacaagc tggacgacta
     3481 atggaaccgg taacactttt caactgaatt tctcccatac ctcgtacagg gatcggtgtg
     3541 atcacaacag ctacttgtcc ttccagagtt ttttcagaag acgacatcgt tgattcggca
     3601 tgtttcatcg gcatcaatat atagaaatta agcaaaaata ccaacactat cgatatgcct
     3661 ccactagcaa taaataaaaa taaagatgat tgatctgtta aacgctcacc tagaaaacca
     3721 aataagcttg tgaaagttaa ccaagggata ataagcatcg gatcaagcgg gccatcaaaa
     3781 tcgaatacat ctccaaaaat aatcaatagg actgccaatc ctgcacaaat cagcaatgta
     3841 taccaataaa tcgtctcaag tgcgattccc cctatcatat acacgcctcc ttccatttct
     3901 acgtctatca taccaatgtt tgaaaattct gtatagcttc ttttaccgac tcagtggtgt
     3961 tttttatagg aaggttgtgt ttatcgttga caatttgtta aatttttgtt ttcatgttaa
     4021 aattttcaca ataaattctt tcgaggtagc ctatgtttcc tttttttaag aaaaaaccat
     4081 taaagctcca gaacacgatc atccttgtcg tttgtttctc cacattcgtt tctttgattg
     4141 tttctgccat attgatcagg aattttattc tttcgatgaa ccgatctagt gtgtcagata
     4201 aaatctctag cattgctaaa atcgttgcca atgataaact catccaaaaa aagctgcaga
     4261 ctggtgaccc gacacatgcg attcaaggaa taacaaatga aatcatgatt gcatcgaatt
     4321 tagattttat cgttgtcatg gaccctgaag gcattcgata ttctcacgca gacccaagtt
     4381 acatcggtct tcctttctca agtcatgaag atgtgaaaaa agtgattgga ggacaagaat
     4441 ttttctctga aaaagaagga atgttagggt tcggcttccg ctatttcgtc cctatttatt
     4501 atgatcaaga gattattggt gcagtttgtg ttggcttaac aaacaacacg attgacaatc
     4561 aagtcaaaga tggacaaaaa atgattgttt tttcattgat tattggactt agcgtaggca
     4621 tcataggcgc cgtttttctt gcccaaaaaa tgaaaaaagt actgcttgga cttgaaccac
     4681 aagaaatagc agaacagtta agtgaaaaaa cgattatcga aaatgaagta gatgaaggaa
     4741 tcttagccat ctcttctgat aaaactatcc tcttgatcaa tacacctgca aaaaacattt
     4801 tctaccaaac agagcagcta ttggatttcg aagtaggaaa aaaatcagtg ataatctata
     4861 tgacgttttg tttaaagaga cgattgagca tcaaacgacc gttcggactc aatccatgta
     4921 tttaaatggg atagaaacta tttctagcgt tacacctctt tttagcaatg accgtttttt
     4981 aggagcagtc gcaactttac gaaatcgatc agaagtaatg aaattaatgg aggagcttgg
     5041 ggggacgaaa caatatataa actccatgag agctcaaacc catgaattca tgaataaact
     5101 tcatgtcatt tctggtttga ttgatttgaa gaagtatgaa gaagtcaccc attttatcca
     5161 gcagctaaat caaaattata aaaaagagat tggccaagtc tcaagtctga tcaaagtacc
     5221 tgcaattgcc ggttttttat taggcaaaat aaacgaagcc aaagaacaat ctgttgaaat
     5281 gctgttagaa agcgattctt ttatccctaa aatggagatg gatgactcca tccattcact
     5341 tttgcaagtg ttagggaact tacttgataa tgcaaaagaa gccgtgctac aaaaacatga
     5401 aagaaacgga caggtccgga ttcatttaaa ctatgacaca gaagctgaag tattcatcgt
     5461 gaccgtctgc gatgacggtg tcgggatatc agaagaaatc tcagaaaaaa tatttacgct
     5521 aggtttttcg acgaagggaa tcaatcgtgg gtttggattg aatctcatcc attcaatcgt
     5581 caaacgccac ggtggatttt tagaattttc caaacaaaca aatggcggta caatcgtcta
     5641 tctagaatta ccaaaaaagg aagatcaaaa tgatgacaaa tgtcctgata atcgaagatg
     5701 atccaatggt tgcagaattg aatcgtactt ttgtggaaag catccctaat atgaacgtaa
     5761 tcgaaagttt cagaaatgta gaagaaggaa aaaaatattt cagacaaaaa aacgcagatc
     5821 tgctgctgct tgatgtatac atgcctggcg aaagtggtat ccatttttta tcttggctta
     5881 gacagcaagg aaatgatatc ccagtgattt tgatcactgc tgcagatgat atcgaaacca
     5941 taaagaatgt gatccgctac ggtgccttag attatttgat caaaccgttc actttgcaac
     6001 gttttcagct ggcgatcgaa aagtatcttg ctttctctca gacaatccaa acgactgaaa
     6061 aagccgatca gaaattgatc gatcaagtat tcaaccacac cactcccatt cacgaaatac
     6121 catcattcat cgatttgcct aaaggtctgt caaaactgac tttaaaaaaa ataagtgcat
     6181 ttattctaaa agaagatact attttttcaa caaaagaact ggctcaaaaa agctcactgt
     6241 cacgtatatc cgtcaagaaa tatttgaatt ttttagtaga acaagatata ttgaacgaaa
     6301 gaatcaaata tttggaaatc ggccggccgc taacctgtta tgaattgaca aagaatgggc
     6361 ggaaaaagct cacagattac tgttgagtca tcgaattatc ccatacaaaa tagtccaagt
     6421 atacagaagt aacaactgtt tacttggact atttttaaac attttattga cgaatatcca
     6481 tgttttctta tcttaattga cgggctttag gaagagatac ctcatttttc gtttttccag
     6541 tttctattat ttcacgaaag ttctcgaaac ttgtcgaaat catatttgat aatgcctcat
     6601 ctgtatttga acctacatgc ggggttacaa gaactctagg gtacatttct actaattgct
     6661 ggatagcagg gtctggaatc gtctcccatg gcttgaagga tcggaaaaaa acctctgttt
     6721 catttgcaaa aacatcggtt gcaaaaccag ctaaatgatt gcttttcaaa gctcttaaaa
     6781 tagcttcatt atcttgaagc tctccacggg ctgtattaat caagatgcta ccttttttca
     6841 tttgagctaa aaatccttca ttgatcattt gatcgttttc acctgggata tgaggtacat
     6901 gaatactgac aatatcgctt ttggctaata actcttcaag cgagtggaac gtcaatactt
     6961 ttttagcagc ttctgtctga tgaatatcgt agccgatcac tttggctcct agtcccttaa
     7021 acaaaacagc ttccgtcaac cctatcctgc ctgttcctat gatccctact gtgcaatttc
     7081 tgatttcacg actaaacatt tgctcatcca ctcgaaaatc tttgaaagat gttcggatcg
     7141 tcgtatacgc agtatttctc aaaagcatca tggcaaaagt caaagataac tctgctatcg
     7201 catttggcga atacgatggt acacgggcta ctgtcatacc aaaatcagct gccgcttgta
     7261 gatcgatatg attgactccc acagtccgtg taaaaacata acgaatccca tattcctgca
     7321 tttttgcaag attggtctca tctgctatac aatttcccct caacaataca gcatcatggt
     7381 cctttgctga cacgatattg tcatgtgtca acagttcttc ataaagactg agatcataat
     7441 ggtaaatatt caatttttca aaaaaatcta tctcgttcgg acgaacaccg taacatgcga
     7501 ttctcatact ttcttcctcc atttcttatg aaaataaacc tatgccatga atcatttgta
     7561 atacaacgat ccatacaggt gtcagtacca cagcaaaaat agttgacagt aaggatgcat
     7621 tggaggctaa aagtgcctcc ctatcaaaac tgatagcata ggctgctgcc actgttgcag
     7681 ctggtgtagc catcatgata acgattgttg ccagtgcttc aaaattgact ggtagaatat
     7741 tcgttgccgt caaaactgct agaatcacga tattcaatgc aggtacaaat accactttga
     7801 ctagactgta ataccatgaa gttttttcag atgcagcttt tttgaagctt acttcaccta
     7861 atgttgcgcc gattgctagc cacgccaatg gtgaacttaa accagctaaa taatccatcg
     7921 actgaaatag ccacggtgca gtctggtcaa ttcgcaaaaa cgctacttgg cttatctctc
     7981 cttctgcacc tgtaactgac acttgtggca gatacccttg gaacagccaa atgaaaaggc
     8041 cggcaaaagt agcgataatg attggattga cgaacatcgc tttgatattt ttagcttcca
     8101 tcttcaagcc actcattttt atgtatccat aagaatacaa aaagattcgg tagccaatat
     8161 tgaaaattga ggaatacatc acgccgacag gaccataaat cgcactgacg ataggcgttc
     8221 cgaaaaaggt cgtagaacca aagatcgtca ggacacgcaa cgttgtttgc ttatcccctt
     8281 tatagcttaa gaaaaaaggt tttgaaacta gaataagaat aatataaata actaatcccc
     8341 aaatcaagac attgattcct tgcatcagta attctgtatt gatatcttgc atgaaagctc
     8401 gaaatgccag tgctggcaaa gccactgtta agacgacttt cgatagaatt tttccaacag
     8461 attcagaaaa aatccccttt tttctacaaa caaaccctaa taatataata aaaactgttg
     8521 aagtgattgc actgacaatc gacatatctg tcaaagtcga tcgaaaaatg tcaaataagt
     8581 tcatctttct cctcctaatt atgttcattt attcacaata aaaaaagctt ttattttgct
     8641 aatacgtatt aacattttta attttatctt tacattgtta cttattgaac aataggaaat
     8701 taaagaaact tttgaaactt atgtaaagtg att
//
LOCUS       DASJON010000121         8676 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJON010000121 DASJON010000000
VERSION     DASJON010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.122"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            201..401
                     /locus_tag="U3989_002018"
                     /pseudo
     CDS             201..401
                     /locus_tag="U3989_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            888..1067
                     /locus_tag="U3989_002019"
                     /pseudo
     CDS             888..1067
                     /locus_tag="U3989_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1113..1718)
                     /locus_tag="U3989_002020"
     CDS             complement(1113..1718)
                     /locus_tag="U3989_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1715462.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            1900..2235
                     /locus_tag="U3989_002021"
                     /pseudo
     CDS             1900..2235
                     /locus_tag="U3989_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2400..2627
                     /locus_tag="U3989_002022"
                     /pseudo
     CDS             2400..2627
                     /locus_tag="U3989_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2753..3724
                     /locus_tag="U3989_002023"
     CDS             2753..3724
                     /locus_tag="U3989_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1715463.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            3864..5177
                     /locus_tag="U3989_002024"
     CDS             3864..5177
                     /locus_tag="U3989_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1715464.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5220..6086
                     /locus_tag="U3989_002025"
     CDS             5220..6086
                     /locus_tag="U3989_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1715465.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6083..6931
                     /locus_tag="U3989_002026"
     CDS             6083..6931
                     /locus_tag="U3989_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1715466.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            6945..8417
                     /locus_tag="U3989_002027"
     CDS             6945..8417
                     /locus_tag="U3989_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN1715467.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8434..>8676
                     /locus_tag="U3989_002028"
     CDS             8434..>8676
                     /locus_tag="U3989_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1715468.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2735 a         1489 c         1783 g         2669 t
ORIGIN      
        1 aacaccacgc atagttttta ttttgagata ttctagaatg aaacacatta agaacgaaag
       61 caaagcaaaa gacgccagaa cttctactac cttctaagtt ttttcatatt tgtttgttat
      121 gctatacata gatctgcata attagacttg cgctgcccgt tattgaagtt tcaggcagcg
      181 ctctttttgg gggatcgata atggaagaag aaaaaatatt tgagaaacgc tggcagctag
      241 catcaagtga acaaagagca agatacaata atttgatcac ctcctacccc actgttgact
      301 agacatataa ggaaaagaaa tacttacttt ggctgtgcca gttagatata gatacctttg
      361 aaacatttga agtgatccta gataaaatta aacagaattg atcagaaaca agcgaagcct
      421 tctctcggtt cgcttgtttt gctatttatt agttggctca gggcaactct aagctcgttt
      481 gaaagaaaac cgatggataa tccccttgtt tgtaccactt tagaatacat ggatgtacgt
      541 attgccattg actcctatta gttactgaaa aaaaatttaa acaagttgag tattgcagat
      601 aaatagctcc agtgaggtga ataactgctc cactcaaggc aatagatagc tccatccagg
      661 gcaacaaact gctccacttt catatttaat agatgaataa ccttctgtat aatagaaagg
      721 catacctttc ggtatgacaa cttatacaga atgttatttt attggttcac tatcgaaaaa
      781 ttttggagct ttatttttcc ggtatttctc aacgaacgat tagtttaact gtgggaagct
      841 ccagacatac gattcgagaa gttacttctc gagcagacag gaagggggtg actgaactaa
      901 ccgctgatat gagcgatact tggctgtagg aatatctttt tccggaaaaa caaacaacag
      961 cacgaggtta ccttcctgta gactgggaat atgttcataa agaactaatg aaaaagaata
     1021 tgacattgaa actccttcat agagaatatg gggtacatgg aacatagaaa cgaaaatgca
     1081 cggtagaaaa gcgaatgaat atagtataaa ttctacaaat cattcgcttt ctttatttta
     1141 tatatggttg gtcgagagag accattttct ttggcaattt ctgcaactgg aactccttga
     1201 tttaacatgt ccaccacagt tttaaagact aatcgttttt gcggatctgt tgagttctca
     1261 gaatattcaa ttggtcgccc cttatatcga ccagcttttt tggccaataa tactccttgc
     1321 cgctgacgtt cacggatttt ctcacgttcg ttttgtgcaa tataacttag caatgataaa
     1381 aacatatcga acatagcagt atctaggagt tcatttccag tattaaaatc taaaaactgt
     1441 gcatctagta tttttaatgc tacttttttc tgtttcataa aggtaactgt attttttatg
     1501 tcttcataat ctctgcctag gcgatccagt gatgcaacaa tgacacaatc accttctcgg
     1561 atataattta gtaatttttg aaactcaggt cgctggatat ctttaccact ggctttttct
     1621 tggaataatt tatcaatgtt aaggtcttcc atcattgcca attgtcgatc taaattttga
     1681 tcttgtgaag acactctaat atatccaatt tttgccataa aaactccttt taaaaaatga
     1741 tataatttac atgtaaaatt tacttgtagt tttactgtta tcatagcatt tttaaaaata
     1801 aattttaagt ctacttataa tttactttgg aggttttgaa tgaatgaaat taagcgcaat
     1861 aaaaattctt ttggtgttta tttaggagag cctagctcgg aacagttaaa taaaggtgaa
     1921 gctcgtcatg ctctatgtcg aaatatattt tatggtagaa aaggcaaact ttatcaaact
     1981 tattacgacg gtatggagga acagctcaac gcattaggat tagtaactaa tgctatcata
     2041 tattggaatt cactatattt agaaaaagtc tttgaacaaa ttaaaacgga agggttcgac
     2101 tgttccgaag aagtgttacg caagcaattt ccattaatta gtgatcacat taattttgtt
     2161 ggcaaatata gtttccgcta tgaaaaagaa ttatcgaatg gaatactacg tccgttaaat
     2221 atagagaatt attgagaatg ttcgtctttt ggttcctacc gtgcattttc gcacggatgt
     2281 tccagatact atttttgaat gtataaaatt gaaaacacaa gaatactaat aaatcaacga
     2341 ttcttgtgtt tttaattttt cactaccgtg cattttcgtt tctatgttcc atgtacccca
     2401 atcttgaaaa attaattaaa gacttagata aagccattga cgacttagtc gtggtgctac
     2461 cggagtatca atgtttaacc agtatcccag gtattggtcc tgtttaggct gctggaatac
     2521 tcgctgaaat tggtccaatt gaacgttttt ctgatgaaat aaaattgaca aagtatgccg
     2581 gcctatattg gcgacagagt caatccggta aatctgaata cgaaaatttt tttacgaaaa
     2641 gcttcacgtc taatatctat tttaaaataa aaatattcct gctaatttgg aatcgtttcc
     2701 ttcttttggt ataataatac ctgaaattct tttgagagga gttgttcgtg aagtgaaaac
     2761 tactatgaag gatgttgctg cgttagcagg agttggtgtg ggtacggtat ctagagtaat
     2821 aaatggagta aaggtgaaag aggttaccaa agaaaaagta atggctgcaa ttagtgagct
     2881 taattatgag cctgatgaat atgccagagg tatgaaaacg aatagtagca atacaatagc
     2941 tcttatttta cctacgattt ggcatccctt cttttccgag tttgcctact atgtagagga
     3001 agctctcagc caaaaaaatt ataagctttt tttgtgtaat gcgaacggca gtgcggaaaa
     3061 agaaaaagaa tatatccaaa tggtcaagca gagtaaagta gacggaatta taggtattac
     3121 ctattcagac attgataaat atgtatcatc aaatttacca tttgtcagta tagaccgcca
     3181 tttttcggaa caggtttctt atgtaacggc agataattat ttaggtgggc aaattgccgc
     3241 tcaagaacta cttactcgta actgtaaaaa cttgtgctat gttgggggtg tttcacctta
     3301 ccctaacgaa acaaacaatc gtaaagctgg tttttacaat atttgtgagg aaaataaaat
     3361 agctgtgaag gtattggata tgcctgaacc aattagcaat ttagaggaac aattgctcgc
     3421 ttttttttcg gataatcctg aaattgatag catttttacc atcaatgatt tcatggcttt
     3481 ggatgtgata aaagcccaaa aaaaactagg gaaggaagca cctaaggatt ttcaaattat
     3541 tggttttgat ggagtcaagc tatcgttgca aaaagactat ttactatcaa cgattgtaca
     3601 gccgatagag gaaatggcca aggtttcagt agaaacactc atggcgatga ttttaaatga
     3661 agaaccgcct aagcgtgtgg tagtaccggt tttctttcac gctggagaga cgactaaaga
     3721 gtaaaaaacc ggtttataac gatttttttg agataggttg acagaaagtg atttcaatga
     3781 tatcttaaaa ccaattaatt ggaaacgtta ccaattaatt gaaaaataaa acggaaacgt
     3841 ttcaaaaaat aggaggaaca tcgatgaaaa agagaatgtt agcaattgca gcaattttag
     3901 cattaacagc tggaggactt gcaggatgtg gcagtaagga gaaaagttca ggtagcagtg
     3961 atccaggaga aattaagatt tgggttcagt tttctgatga aacgcctgaa ggaaaagctt
     4021 gggcagagat tgtgaacaat tttaatgatg aatacgaggg tgaatataaa gctgttactg
     4081 agtatattcc tcgtagcgga agcggcggtg gatatgagga taaagtaaat gctgcaatta
     4141 caaccaatac ccttcctgat gttattacgt tagatggacc gaatactgca gcttatgctc
     4201 attcaggagt cattgctcca ttagatgatt atttgaaaga tgcggacatg gatgatgtat
     4261 tagacagcat tattcagcaa ggaacctacg atggcaaaat gtatgcgttt ggtttctcag
     4321 agtccagtgt gggtgtctat tacaataagt cgctgttcaa agatgctgga attacagaag
     4381 atcaattgcc gacgttagaa gatccttgga cttggacgga atttcaagca atttgccaaa
     4441 agctgactga taaatacaaa acgcctgcaa ttgatatgca aatggcgtcg ggagacgaaa
     4501 tgctgaccta cgcatataca ccatttatat ggtcgaatgg cggcgatgtc gttaatgctg
     4561 atggaaccaa agccgaagac tattttaacg gtaaaaaatc cgttgaagcg ttggaattta
     4621 ttcaagaatt ggttgaaaaa ggttacacca caatcagtcc tattgaaaaa ggatttgaaa
     4681 ctggcaaata tgccatgatg ctgagcggtt cttggacaat ggcagattta gaggaaaact
     4741 atcaagagat tgattatggt attttgcctt accccgtttc agatgcaact ggggagttag
     4801 tttcaccaac cggaagctgg cagttagcaa tgactgagaa aacagagaaa aaggatgctt
     4861 ctgcggcctt tattaagttt gcaaccagta cagaatctag tcgtattatg agtttaggga
     4921 acagtgttct acctatcaga aattcaacag ttgatttaat taaagatgaa gtgtcggaag
     4981 gaatgcaggt attaattaag caaaaccaac tttctggaca tgcccgccca gtggttgtag
     5041 cttatccgca agtatctaga gcattccaac aagcagttca agatattagc tacttcgagg
     5101 aaaacccaga tgtacaaaaa gtagcagata gtcgcgctgc tgaaatgcaa caggcaattg
     5161 atacttcact aaaatagaag tttctaaagg gggccaatca agtgttaaaa aaaccgacaa
     5221 tgaaagacaa tttgataggt tacggctttc tagcgccagc tttgctgctg ctgacgattt
     5281 ttctgatcat acccgtaatc atggtaattt attatgcctt tactgattat tatttgttaa
     5341 cgccagatgc tagacaattt atcggcttag aaaattttaa gcgactgttc gctgatccaa
     5401 tttttgtaaa aagtatttgg aacacggcta aatttgttgt ttggattatt ccggtccaaa
     5461 taggggcagc tttaggaatg gcacttatta tcaacaaaaa tcgtaaagga aatatctttt
     5521 ttaaagtagc cttttttgcg ccagtcgtga tgtccttagt cgttatttcg attttatggt
     5581 tgtacttgtt aaatcctaat gaaggtctgg ttaatacgct atttgcaaag attggaattg
     5641 cttcgcagcc ttttttgact agtcccaaac aagcaatgta tacgcttgtt ttcgtatcgg
     5701 catggcaagg tgcaggatat cagatgctgc tgtttttagg gggaatgcaa aatattccgc
     5761 aagacgttta tgaggcagcc gaaattgatg gtttttctaa gtttcagcaa tttcgctata
     5821 tcaccatgcc attattaaag cccacggcga tttttatttt acttaccaca ttgatttcgg
     5881 cctttaaact aatcattcag ccaatggtaa tgactcaagg tggaccaatg aattctacta
     5941 tgacaatggt ttattacatt taccaaactg gatttacaga ccgtatggta ggctactcca
     6001 gctccattgc gttaatcttt accactatga ttggtttaat tacaattgct caaagaaaat
     6061 tggtaaagga ggatgatcca gcatgaccaa gaaatttgga ccgctaatga tcgtagaata
     6121 ttgtttgctg ttccttttag cggcattatt cattttcccg atgctgtgga tggtagtttc
     6181 ctctatgaag cctgaagcag atgtctatca aaatttagct tccttcaaag cttttttacc
     6241 tagtttgaat ccagctaatt ggtttaaaac ttatcaagag gttatttccc ggttcagtgt
     6301 gggaacttat ttattaaaca gtatttttta tggtttaacc tttgcctgtg ggtcaatcat
     6361 tgtaaactct ttggcgggtt ttgcctttgc gaaggttaat ttttcaggca agaaaattat
     6421 ctttggagct ttgttagctc tgctgattgt cccgatggag acggttttaa ttccgcaatt
     6481 taccatcgtt aattcactag gcttagtgaa tactcgcttg gcagtgattt taccagcgat
     6541 ggccagcgcg tttaatatct atctgtttag aaatttcttt atcgcgattc cagaagaaat
     6601 cattgaatca gcgaaattag atggtgcaaa tattgttaaa attttctttc acatcatgct
     6661 gccaatgtct aaaccagcca tagccacagt aggtgtctta tcctttatct ccagctggaa
     6721 tgattatatt tggccgttaa tggtcttaac tgacagcagc aaattttcaa tgcaagtggc
     6781 aattactacg atcaatacta cacagccagt ttatatcaat caagtgatgg ctgttttaac
     6841 cattgcaacg attccactga ttattgttta tatcgttgca caaaaatata ttctgcaggg
     6901 acttggcggt tctggaacgg gtataaaata ggagtgaata gtgaatgttg caaaaacttt
     6961 taaaggccaa tgagtttatt gagaaaaatg ctcaaacagt caatcaagaa taccggaata
     7021 aatttcattt gatggctccg attggctgga tgaatgatcc aaatggtttt gtttattatc
     7081 aagaggagta tcacttgttt tatcaatatt atccttacga cagcatctgg ggaccaatgc
     7141 attggggcca tgctaaaagc aaggatctaa ttcactggga gcatttgcca gtagcactgg
     7201 caccaggtga aagctatgac aaggaaggct gtttttccgg cagtgccata gaaaaggatg
     7261 gcaagctgta tttgatttat acaggacaca atgttgtgga agggcaagtt cgacaagttc
     7321 agtgtttggc agtttcagaa gatggcattc actttgaaaa atatgccgga aatccaatca
     7381 tcgcagaaga tcacttagct tctgttgcaa cgaccgaaga tttccgcgat cccaaagtgt
     7441 ttcagcgaaa agaagactat tttactgtag tcgctactaa aactgcagat gctcgtggac
     7501 aaattgtgat gttccattcc aaagatttac taacttggga gttttattcc gtattgctgg
     7561 agggaactcc agaacaaggc attgtatggg aatgcccaga cttgtttgaa ctagatggca
     7621 aagaagtctt gattctttct cctattcaaa tgactccaca aggcaatgct taccaaaata
     7681 cgaactcaac tgttgctttc atcggtactg tcgattggca aacaggcaaa tttctagtag
     7741 agaattttca tgaaatcgat ggcggtttag atttttatgc accccagacc tgtgaaaacg
     7801 aagcaggcca aagaataatg attgcttgga tgcaaatgtg gcagcgtaaa atacctacga
     7861 atgatctggg ccatggttgg tcaggttcca tgaccttgcc tagagagctg agggtggctg
     7921 ataatcaatt gttgcaacgg ccagtttcat caatttatca agtaattgat ccgcaggaaa
     7981 ggattgaatg ggcgaagact gaagagaatc gcttcttact gaaggactta gtatatgacc
     8041 agcactattt acgcttggaa atcaatttaa aacaggcaac gaaactagaa cttcactatg
     8101 cgaagggacc cgaccataca ttactgctta actatgatgt tgaacgggag tgctttactg
     8161 cttccaggga acacatgccg tatgatttat caggagctga aaaggaagtg ttgaaaaagc
     8221 gcagtgtgac gattcctttg gaaaataatt gtctaaccat cgaaattttc cgtgatactt
     8281 cagctattga gctttttcta aatgatcgtc tgactatgag tacaactttt tatgagacag
     8341 aaaaaggaca ggattttctt ttgctggcag acagtgaagt aaccttttca ttggaaagcg
     8401 gcttaattaa tctatagaag ggagagctca gtgatgattt atggttcaat cgaagcaggc
     8461 ggcacaaaat ttgtgtgtgc cattagcgat gaagaaatga cgatcaagga aagagtgagc
     8521 tttccgacaa cgacacctga ggaaacaata cctttagtca ttgacttctt caaacaatac
     8581 caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
     8641 tccgccactt atgggtacat tacctcgacc ccgaaa
//
LOCUS       DASJON010000122         8590 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJON010000122 DASJON010000000
VERSION     DASJON010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.123"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..443
                     /locus_tag="U3989_002029"
     CDS             <1..443
                     /locus_tag="U3989_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HEN1715469.1"
                     /translation="AAPDIVDVTKAELLDKDKNPVDMLEQGEKLYVKVTYEVKDDTIK
                     NPVLGIALRTVDNFYVCGLNTLLDGIKIPWKKGENIFYLEYEKLGLLAGEYYFDVAVF
                     EENATVPLVYKTKYMNLFVSGSYIGEGIVVLDHKWEEGTHSNEI"
     gene            433..2019
                     /locus_tag="U3989_002030"
     CDS             433..2019
                     /locus_tag="U3989_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1715470.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            2058..4196
                     /locus_tag="U3989_002031"
     CDS             2058..4196
                     /locus_tag="U3989_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1715471.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(4564..5547)
                     /locus_tag="U3989_002032"
     CDS             complement(4564..5547)
                     /locus_tag="U3989_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN1715472.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            5925..7316
                     /locus_tag="U3989_002033"
     CDS             5925..7316
                     /locus_tag="U3989_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1715473.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            7332..8372
                     /locus_tag="U3989_002034"
     CDS             7332..8372
                     /locus_tag="U3989_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1715474.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            8391..>8590
                     /locus_tag="U3989_002035"
     CDS             8391..>8590
                     /locus_tag="U3989_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1715475.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPK"
BASE COUNT         2920 a         1412 c         1680 g         2578 t
ORIGIN      
        1 tcgccgctcc agatatcgtc gacgtgacga aagcggaatt gttggataaa gacaaaaatc
       61 cggtggatat gctggaacaa ggagaaaaac tatacgttaa agtaacttat gaagtaaaag
      121 atgatacaat caaaaatccg gttttaggaa ttgcgctacg aacagtcgac aatttttatg
      181 tatgcggact taatactcta ctagatggta tcaagattcc ttggaaaaaa ggagagaata
      241 tcttttatct ggagtatgaa aagttaggtt tgttagctgg tgaatattat tttgacgttg
      301 cggtctttga agaaaatgca acggttcctt tggtatataa gacaaagtac atgaacttgt
      361 ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt agaccataaa tgggaagaag
      421 ggactcatag caatgaaata tgattttgaa atggacctag atgaacaaag cagtgtggga
      481 aaaattgccg cacagatcaa accaggaagt aaagttttag agtttggtcc cggaaacggc
      541 cgattaacga aacatttaat cggagcaaaa caatgtgaag tgagcattgt tgagttagat
      601 aaggaattgt tcgattttgt cagcgaattt gctcaagatg gattttatgg tgatatcgaa
      661 agttttgagt gggcaaacta ttatgctgga cagacatttg attacgtctt gtttgcggat
      721 gttttagaac atttggtaga tcctggaaaa acattgaaga aagtcagaga atttttgaat
      781 gaagaaggag agatcctgat tacattccct aacttggcgc ataattctgt catgatcgat
      841 ttgttcaata accaattgcc gtgggcttct tatggtctcc tagatgaaac ccataattct
      901 ttttatactc atgatggctt ccaaaaagtt tttgaaaaag cgggattatt cattaatatt
      961 gaagattatt tgtacttagc tgtaggtgat acagaattaa aatcaactta tgaagaattg
     1021 ccggaagctg tacgctatga tttcaaaatg cgaccatttg gtgaagtcta ccaatacttt
     1081 ttctcattga tgaaacatcc agtagcacaa agttccattg cagagccaca aaattctaat
     1141 tatgtaaaag tgttagaagt aacccagcaa acgaaacaag atgaaacaat ccaacaaata
     1201 ccattcaata atttcacagg ggaaaatgag acactaagtt tccccgtatc agaaacaaca
     1261 gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta tcgaattcag cgcagaagct
     1321 gccggagaaa aactaacatt tatcgattcg aatgctgtag tcaaaacagt aaatgattgt
     1381 tatttgtttg atgggaaaga attaccagag ttcgttctaa cggatatttc tgggaaagaa
     1441 gtgactatcc attgtcacta tcgctttatc ggggaattga caccaacaat gaaagaattg
     1501 ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc tttctgagaa aaatgacgaa
     1561 ttagaaagag aaaatcatca tcttcttgag gaaaatacaa gactagacca tacgttaaaa
     1621 acaaccacaa atcgctattg cacattgttg gaaagtgatg aatggacaat aaaacataga
     1681 ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag aaaaagaact ttctatatgt
     1741 attgacgaga aaatctggga tgcagaaaca aaaatattaa aaatcatcgg atggggaatc
     1801 gctaattcgg atcgccagcc cttgtcctat aagttatcag ctgaccaatc gccatttttc
     1861 gaagcaattc ctgtttcacg agaagaagtc aatcaagcag aacagctcgc aaaaggaaca
     1921 gaagcgggat ttgagttacg aatcctttgc gagcaagaac gttcattctt agtggaagca
     1981 gtagctcaaa atggccaatc atggatcatt gaaatgtaaa taatgattaa cttataaaga
     2041 taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt atttatcgat caaatcactc
     2101 gtgaacgggc cactggcgat ttgcttattg tgggctgggc aattgatgaa gtgaccaaag
     2161 aaatccctac gataaaggta gaaaaagaaa atgtgattgc tgaagcaaca catgtcgtaa
     2221 gattggatat caatcattta tataatcttg atgttaagac ccaaagcggt tttaaggtac
     2281 gtttatcagg gaaaatgaga ggaaaagcca tcttagactt ccaaacagcg agacatcaaa
     2341 atggcatagc tgtgaaatta aacggtaaat acccttatga tgacggcatt gaatcttcat
     2401 gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta tgcaaggact catggcgtaa
     2461 aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg atcgatcgac tatgcagagt
     2521 ggatcagtcg tcatgagaaa ccagatttga aagagcaaag gaaagaagca caaggatttg
     2581 cgtaccgtcc attgatttcc atacttatgc ctgtctataa cgttgaaatc aaatggctcg
     2641 aaaaatgcat tgattcggta ctggatcaaa cgtatgatca ttgggaacta tgtatcagtg
     2701 atgatgcttc tactgatcca gctatccgaa agtgtctgga atcctatcaa gcaaaagatg
     2761 atagaatcaa agtcgttttc cgaaaagaaa atggacacat cagtctggca actaattctg
     2821 cattggaaat agcagaagga gaatttatag cactcttaga taatgatgat gaattaccgc
     2881 catttgcatt atatgaagtg gcaaaagtgc taaacgtaca tccagaactg gatgtgatct
     2941 acagtgacga agataaaatc gatgcagatg gcaatcgctt tgatccgcat ttcaaagcag
     3001 actggtcgcc tgatacattg atgggaaata attatatctc tcacttaggc gtatatcgaa
     3061 caagcattgt taaagaactt ggcggtttcc gtaaaggcta tgaaggttct caagattatg
     3121 atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca tatttaccac attgaccgag
     3181 tcttgtatca ttggcggacg atcccaggat caactgcaag taatggcgaa gccaaaagtt
     3241 atatctatga ttccggagtg aaagcattga cagatgccct gtctagaaga aacatcaaag
     3301 gaagcgtacg tcctggcaga atttctggat tttatgagat tgcttatgac gtcttgcaag
     3361 aggatttggt cagtgtgatc attccaacga agaatggcta tgaagatcta aaaacttgtg
     3421 tggattcgat catcgaaaaa acgtcttatt caaattatga aatcattatt gcggataacg
     3481 gaagtacaga tccaaaaatg caagaattat ttgcagaata caagcatcag ctgaaagacc
     3541 gctttatcgt agaattgatt gacattccat tcaattattc tcgaattaac aatttagcag
     3601 cagaaaaagc aaacggcaaa tactttttat tcttgaataa cgatacagaa gtgattgagc
     3661 cagattggat gacggtgatg gtttcttatg cacagtttga tcgaatcggc tgtgtaggag
     3721 caaaactgtt ttatccagat gatacaacgc agcatgcagg agtattatta ggaatcggtg
     3781 gtgttgcagg gcatgcattg aataactatg atcgaacaca ttgtggttat ttcggccgat
     3841 tggtcattga cgtcaattac ttagcagtca ctgcagcttg tatgatggtc aaggcagctg
     3901 acttcaatgc tgttaacggg tttgatgaaa cattagaagt agcattcaac gatgttgact
     3961 tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta tgcacatcaa gcagaattgt
     4021 accatttcga atctaaatca agaggttatg aagatactcc agaaaaacaa agacgttttg
     4081 ctggtgaaat caaaaaaatg caagataaat ggccagcata tattgcgcat gatccatttt
     4141 acaatgataa tttaacaaaa gaaggaatcg gcgacttttc gttgagacca gattaaacta
     4201 taataaaaag ctgttatgtt gtgattcgct cgcacataac ggctttttat agttttttgt
     4261 caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg aggttgggac agaagtgttt
     4321 aactccgaga aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttcgg
     4381 cttatttaag aagaagttgc ttctgctccc accgtttatt cgtttttaaa gaatgagaca
     4441 aaagtgtttc ttacttttgt cccacgctct tattcatgct cagagcgcca atagatacaa
     4501 aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa tcgatatttt attttaatat
     4561 aggctatttt aaaataaatg gtttaaggta ttcctctaca aaagccactc tatcaattat
     4621 catgggttta aaataagaag tgatggagat aaccagattg ttcttagcgt caatataaag
     4681 aacatttcca ctattaccaa ttgctgaata tatttcgttc tcagaatcta atacccacca
     4741 taaatagcca tatctcatat tttgtaagtc ttctaaagca ctggttctag gtttgaacat
     4801 ctcgtttatc cacatgggtg gtacgatttg ttttccttta tacatccctt tatttaggca
     4861 taatacacca atttttgcca gatcacttgc cgataaacac aaaccatatc ctgccgtccc
     4921 gatatttagt ggatcacaaa accagacatt ggttttagga gacttatcca ttgtaaactg
     4981 cttatgttct tcagctgttt cagcataata attgacatgg tttggtatcc ctataggatc
     5041 aaatagatac ttattggcaa aatcaacagt tgttagctta cttgtctgat acaacaagcc
     5101 agttaatatg tgtaaacaga cagtttgata gttgaattcg ccggttatac cttttcttcc
     5161 tcccaataaa tctagtgatg tttttgtcca atcttcactt gaacagactt ttgtccatgg
     5221 atcgccttta catttgtagg gtgctttcat tgttagcaag tgcttgattg taatagcgta
     5281 aatagttttc tccccgcgtt tcactttata ctctggaaaa taatctaata ctttatcatc
     5341 gatacttttg attaatcctt gctctaaagc aatgccaatc agcaaagata caatactttt
     5401 cgttgctgac atgatatgac aagtatcgtc ttttttataa ccattccatt catctgtata
     5461 aacttctacc ccatccttat atgcgacaat ttgacatata tttggttgtc gaaaaccaat
     5521 aaaatcatgc aactcttgtt tattcataag atattacctc actttcttct ataagcataa
     5581 tgtcattaaa aatcgatttc aattttttcg attcttcacg taatacaggt attcttatgg
     5641 actagacagc gctttgggaa gatggattct ctgaaaaata taaaggttca ctttatgagt
     5701 ggataaaaaa agcagcaaat acttcgactt ctttaattta ccaacattag tgaaaatcga
     5761 ttcatctgga gatttgtaat ttagagtcct tgtcttgatt agaggtgtat aaagcttttt
     5821 attagacatt tttaaattcc attacaattt gcttataaac taggttttta aaggtgacta
     5881 tggtaagata atagagatat tattttgtga ttaggagtgt atatatgaat aaaaatggag
     5941 aatggagtgc tgcacgacgg atattcatta tattaatgga tgttattgta tacaatgcag
     6001 caatatatag tagtttctta ctgaagtttc gtggagagat cccattacga aatttagaaa
     6061 ctttccaaca ctcagcaatt attatttcag tgatttttat tgcgttgaac attttgttgg
     6121 gtgcatatgt tttttataat cgaatgatca gtgacatcgt gtttgtcaca gtcatcggtc
     6181 aggtattgat gacactaggg atcatggtag tgacttttgc gggccgctgg tttgcttttc
     6241 cacgtcttgt tatcttactt tcatttgttc tcggtacgct tttcttaagt atctatcgta
     6301 ttctcgtata taaactttat ttacgcgtaa gtcgagataa aaaggtcgtc attattgggt
     6361 tagaaaagga tgtcgcacca gcaatccaaa attttggttc aaagaaaaac aacaaacata
     6421 aagtcgaagc agtcattctt gataactatt atgcaaacat caaaaatatg attaaagaca
     6481 tcgatattgt ctatcttgct tctcatatcg aagaaactga aaaaatcaaa atctatcaat
     6541 tagttactaa aaatgaaaaa aaattgtttt tgaatacaac atttgaaaac ttgacattga
     6601 tcaatccaaa tattatgaat ttcgaagatg aaagtattat tgaagcatct ggatttagaa
     6661 tccaacctga atatgaacta ttcaaacgtt tgtttgatat catcgtttca cttgttttat
     6721 tagttattgc ttcaccattt atgttattca cagcaatttt agtcaaagtg acatcaccgg
     6781 gaccaatcat ctataaacaa gtgcggatca caaaaaatca aaaggaattt tcaatctata
     6841 aattccgctc gatgaccgcc acagccgaag caaaatctgg accagtttta gctaagagca
     6901 atgatgcacg agtaacgcct gtaggtaaat ttattcgagc tgtccgcttt gatgaattac
     6961 cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt aggaccacgt cctgaacgtc
     7021 ctttcttcgt cgatcagttc aatgcagaaa acccttatta ctacttacgg cataatgttc
     7081 gtgctggtat cactgggtat gcacaagtct atggaaaata tgtctctgat tacaacagta
     7141 aattacgttt tgatttactt tatatcaaaa aatattcatt gatgcttgat ttaaagatct
     7201 tgctgcaaac catcaagatc ctatttgata aaatgtcctc tcaaggactt gaagaagacg
     7261 aaaatgtgat ctcattggca gaaattgctt ttccgaatga aaaaatcttt cgctagttgg
     7321 gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt accagtatac aatgcagaag
     7381 caactattgg tcgaacgatt gatattctaa aagaattgga ttatgaagag tacgaagtcg
     7441 ttttagtaaa tgatggatca acagatcaaa cacaggaaat cattcaagaa ctgattgaaa
     7501 atgatgaacg ttttgaattg attactacga ccaatcaagg gcccggatta gcaagaaacg
     7561 aaggaataag acagtcaaaa ggagaatatc tcttattctt cgatgcagat gacacgccag
     7621 taaaaacgat cttgtcagat tatgatcatt tgctagaaaa gagtccaaaa gcggatcttg
     7681 ttgttagttc gtttgttttt cgtacaatgg acgagggaag aatcgtttcc gaaaaagaaa
     7741 acttagttac agaatgtcac tatctaactc acgaagagtt tataaaagac atgtatcagt
     7801 taatgaatga acagttgatg tatgttgtat ggaacaaatg ctatcgtagc gatattctga
     7861 aaaataacca aattctattt aaaacttata atagttgtga ggatcgaata tttaacttac
     7921 attattatga atactgtcat caagtgatca tgaacccaaa agtagaatat atttatgaat
     7981 ttgaaggtgg gaaggggatc actaatcaat accgacctaa caagttcgaa acatttaaag
     8041 aattctattc attagcaaat actgtaacag acgatattaa taaagacggt atggcggcgc
     8101 ttcttttaaa agggacaact tcagttattt tttctattta tagtacctct gcaagaactt
     8161 caaaagagaa gaaagacgaa gcaaagcaga tattgagtga tccagtgatc cgagaagcca
     8221 agcaaattgc taagacggat tcttcagcaa aaaaaataac aaagttgctt tacaatatgc
     8281 cagctccact atttttatct gctgtaaaag ttggaagctt cgttgaagtg aaaatgccag
     8341 ggttaatggc tgctttgaag cgagtatatt agttaatgga ggaaaatata atgaaaagaa
     8401 ttttgcattt tcagggacga atgggtttag gtggagcaga gtctttcatg atgaatttat
     8461 atcgtaaaat tgatcgtaca aactatcaat ttgatttttt gatttatgaa gattatgcag
     8521 atgttcaaga ctatcattca gagattgaaa gattaggagg ccgtattttt gttgtaccta
     8581 atcctaagaa
//
LOCUS       DASJON010000123         8586 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJON010000123 DASJON010000000
VERSION     DASJON010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.124"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..868)
                     /locus_tag="U3989_002036"
     CDS             complement(<1..868)
                     /locus_tag="U3989_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715476.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKY"
     gene            1051..2175
                     /locus_tag="U3989_002037"
     CDS             1051..2175
                     /locus_tag="U3989_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1715477.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            2200..2379
                     /locus_tag="U3989_002038"
     CDS             2200..2379
                     /locus_tag="U3989_002038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715478.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            2402..3319
                     /locus_tag="U3989_002039"
     CDS             2402..3319
                     /locus_tag="U3989_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1715479.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            3312..4145
                     /locus_tag="U3989_002040"
     CDS             3312..4145
                     /locus_tag="U3989_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1715480.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            4228..5346
                     /locus_tag="U3989_002041"
     CDS             4228..5346
                     /locus_tag="U3989_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1715481.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            5340..7238
                     /locus_tag="U3989_002042"
     CDS             5340..7238
                     /locus_tag="U3989_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN1715482.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            7232..8455
                     /locus_tag="U3989_002043"
     CDS             7232..8455
                     /locus_tag="U3989_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1715483.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
BASE COUNT         2933 a         1280 c         1708 g         2665 t
ORIGIN      
        1 tatatttttt aaaagctgaa gaaaaatgat ggatcgaatg aaatcctaat atatctgcaa
       61 tttctgtata ggttaattca ttttctagca cgagacgttt tgctttttcc atttttagtt
      121 ctttaaaata gctaataaca ctttttccat tcacttttct aaaatgtttt tttaagctgg
      181 ctggactgat aaaaaaaatt tgggctaatt gttcaagcaa aaaattttct gaaagatgtt
      241 cttctaaata tcgtatgatt ggatcaacta attcatctga ataaagaata tgcttttttt
      301 taggaatgac tttctcattt gaaggtattg tcaataaagc aagttcctgt tgataatgcc
      361 ttggtaagga agacaattga attttttgca gacaataagt aactaataag gaaagcaaat
      421 gaatgctcat ctgttggcat agtatgaaaa aagaattgat ttcctgatca taggaaatag
      481 ctggcttact atgttttaga taatcaattt tgaactgttc atttttcaga aaagtaatga
      541 tagatgttat ttcttgatct atagaaaaag attgtcccgt aatatgtgct aataattgct
      601 catcaaaata aaactggata atgataaaag tttgtgtttc cttttgagta gaaaagattt
      661 ggacttcttg ctgtttatca atgaataaaa gggaaggatt atctagttct atagatttat
      721 gtgcaaattc agcatataaa ttagcgcttt catttattag tagtcgaaaa tagggagcag
      781 cagataaatg aagatgggta tctatggaat aggttagtgt ttttttgaca agattggagg
      841 attggttgtt tgttaacaaa agaaccactt tcctttctct ttttatataa aaagctttct
      901 atttataatt aatgactatt ataacaaaaa tgaaaagcca aaacgacaag taaaatattt
      961 ttttatataa atatttgtgt tactaattcc gttaatatac ataagtgaaa gcgttttata
     1021 taagaacttg aaaagaaaga agggatagac atgaaaaaaa gatacaaatt ggtgatagct
     1081 ttgctaaaca ttaccctatt attcagctta agtgcatgtg gaaaaactaa cgaagaagta
     1141 acagcaaaca tcgcagatcc aatgataaaa aaagccacta cattagaagt atatgtcgca
     1201 aacggcaaac tgacaaacta tgttgaaaat gctgcaaaaa tctataaccg caaaactgga
     1261 gcaaatattg acttaaaaat tgtgaatgta gcttcgggaa cagcaacagt acaaatgatc
     1321 accccaaaat tggtaacaca cgaagatatg ccggatatta tctccatggc agacacttca
     1381 gcagcaggtg tgctggaaaa atttcaagat gctttttata gtgctgatga atatggattt
     1441 tatgacaaat atggcgatca gttttatgat caaaaattga atattttgcg tcaacaaacg
     1501 agtaatgaaa cgattattcc ttgggctagt gattttaccc cagcactttg ttattatcaa
     1561 ccaaaaaaat ttgctgaaat aggtgtagat tttgaagaaa tcagttcttg ggaagaatat
     1621 atccaggtgg caaaagcatt aaaagaaaaa actggtagtt ttggaattgc cttgccagaa
     1681 tctggggacc aagaattgtt tatcaacatg ctagcccagc agaatcaatc tttgcttgat
     1741 gaagaaggaa atatccgtct gaatactaaa gaagcgaaac atgctgcaaa gttaatcaaa
     1801 gaaatgatcg actcagatat tgtacatttc tacggtgcac aagatgctga gaaagcattt
     1861 caagaatctg caatgtttgt tgctggcggc tggtatgcta cgaatatgtc tttgaatttt
     1921 cctgatgcat ctgacaaatg gcgcatggct ccactgattc ctttttcaaa agaaaatcca
     1981 ggaaaaggtg cagtatcagg aggatcaagt ttttatgtcc ctaaagatgc caaaaatcca
     2041 caagtggcac aacaatttct tacatttatg ctgacagatg aagaatgctt agccaacgca
     2101 ctggagctgg gcgtggctac atctaactca ttggcctatc aaacagaagc agctgagaaa
     2161 aagtttgact actaaggagg acaaaaatac tatgaactat tgaataaaat caatgaaaat
     2221 acatcaaata ctgttttacc agccacttat tcagatgcta cgtcttattt gactaccgct
     2281 gcatataact attggcaaac aggtgatttt gaaaattcat atctaaaaga agcagaaaat
     2341 tttgctcata agtacggcgt aaaattaact ggtgattaaa aaatattagg aggacttaac
     2401 gatgatgcaa tcttcgttac caagcaagac aataaagaac agcaagaaaa aagcaacaga
     2461 tacgtctcat aaaaaaagtg cttatggatt ggtcacacct tttgttattt tctttattgt
     2521 gacgattttg attcccatca ttatctcgtt tgcttttagt tttatgaaaa ttggctactc
     2581 atttgaattt gttggatttg acaattatct agctatcttc cgagatccat tgtttattaa
     2641 atcttacacg aatgttttga ttatcatggc aggatcaatt ccaataacga tgctattagc
     2701 tatttacttt gctgttttat tgaattcagc acagttaaaa ggaaaaggat tttttagaac
     2761 tgtctattat attccaacca ttacatcaac ggtggctgta gcttccgtat tcatgacttt
     2821 tttcaatccg actgggttat tcaatggatt tttgaatgcg ctaggaatta acggtgtaca
     2881 gtggttaaca aatccttttt ggatacgaat atcaatgatc atcacgatga tttggcttag
     2941 tgtaggatat aacactattt tatttttagc tggtcttcaa gggatttcta aagagatata
     3001 cgaaagtgct gaaatagatg gagcatcgaa atttcggcaa ttctttttat taacgatccc
     3061 catgctaaag ccaatcattt taatggccac agttttagcc actatcaatg gcttgggtaa
     3121 tttcaatatc ccaaatatat tctttgggac ttccaatgga ccagaaaatt cagctttggt
     3181 cgtaggagtc aatttatata aaacaagttt tgaaatggtt gattttggaa aagcctctgc
     3241 tattgcctgg acgatggtgt ttgtatctgt tatattatcg gctattcaat ttaaattcgg
     3301 aggtgataaa aatgactaaa caaaagatac aaagcagaat ttttacgtac gtcattattg
     3361 tgtttggtgc cttaatattt ttgatgccat atttatatat gattgtagct tcaacgcaaa
     3421 gtaatcaaga aattttaagt ggcggaatca atttcaagtt tggggatatg ctattaaaaa
     3481 actggcaagc catcatggcg agatataatt atcccttgat tatttggaat tcgattgtta
     3541 ttgctgtaat cagtacagtt tcagctacag ccagttctac aatggcgggt tatgcattag
     3601 caaagtatcg ttttatgggg aataaactgt tgttcaatac agttatgcta tctaggatgg
     3661 tgcctttttt taccacattg attcctttat tttacattat gagtcgttta ggattatcga
     3721 atacttatgc aggtgtgatc atcccttcga ttgccagtac aacttcagtg tttatgatgc
     3781 gacaatatag cagtcaattt cctacagcac tgatggaggc tgccagaatc gatggtgcca
     3841 atgagtgggt gatttttacg aaaatcgctg taccgatttt atcacctacg attattacta
     3901 gtggtttact tatctttatg ggaagttgga atcaatatct atttccatta gtcatgcttt
     3961 cagataaaga taaatatacg gttcccttaa ttatcaaaaa tatgacggtt tctagtacag
     4021 gtgagccaat taattatggc gcaattatgc taattttagc aacttcagtg atcccaatgg
     4081 tcttgattta tgtgtgggcg cagtctaaat ttaaggagaa tgacattgga gctgcaaata
     4141 agtaaaaaaa ataacgaatg ttaaccatct agttcgttct aggagcaact ccacattcat
     4201 aaaaattaaa agaatggcgg gatatccatg aatgaacaga cgaaggaatg gtcaagaaaa
     4261 attttgaatg tagcttctga aaagatagat gcggaattag acagaatggg gaataaaatt
     4321 ccatatattt cttacaacgg aaaatataca gacgtaataa aggacagagg aatcgactgg
     4381 tggaccaatg gtttttggag tggtatttta tggcagctct ttcacaagac aaaacgagaa
     4441 aagttcaaag aggcagcaat acaacaagaa caattattag aatctgccct gtaccatttt
     4501 tcagacgttc atcatgatgt aggtttttta ttcttaccat cggctattgc acattatcaa
     4561 cagtctcaaa acaaacatag tcgacaacta gggttacatg cagctacggt acttgctgga
     4621 cgctataata tttcagggaa ctatttagta gcatggaatg accataaacc tggttggatg
     4681 attattgata gtatgatgaa tttaccatta ctgtattggg cttcgaggga aactggtgat
     4741 ccgaggttct ctaaaattgc tcggcagcat gcagatacaa cagctaagta tctagtgagg
     4801 aaagatggat caaccgggca tatcgcagaa tttgatgcag aatcagggaa atatattcgg
     4861 ttacttggcg ggcaaggata caatgagaat tcttgttgga gcagaggaaa tgcatgggct
     4921 ttatatggat tcgcgttaag ttatcgttac acaaaaaatc cgtactatct agctatagca
     4981 gaaaagatag cagtaaattt tattcagaaa atcgaaaaaa ctggttatat tcctctcatt
     5041 gattttgctg taaaagattc gaatattgat accagtgctg gagtatgcgc tgcatgcggt
     5101 cttctggaat tagcaggata tgtttcctct gtacgatcaa aaatgtatca tgaagctgct
     5161 atcaatatac tccaaaagac agaaaagcac tatgcaaatt ggaatatgga tgaagatggg
     5221 atcattggag gcggagcaga agcataccat agagaactga cttataatgt gccactgatc
     5281 tattctgatt atttttttat tgaaggctta cttcgattag aacagtcgga attagctatt
     5341 tggtagtaaa aagaggagaa aatacaatga aacatccagt accactatta aggaccagac
     5401 aggattactt tgacttattt aaaaagataa atcaaccact ccagccgttt tatcgagcaa
     5461 gtggcgcaca aattgattat atacatcaag gggtaggata tggaaataaa attgcaggaa
     5521 tggaaggatt ctctcgggtt ttatggggag cggggcccgc aattgaccaa ttagatgaac
     5581 gatggttgaa attaattcta tcaggaataa aagctggaac agatccttcc catcccgatt
     5641 attggggaga aatacatgaa agagaccagc gtatggtaga aatgcccgca atgttattag
     5701 cgttatatca tcatgaacaa ttattatgga aaaaattaga tacaggagaa aaaagccaga
     5761 ttattcattg gttctcgcaa atttttgaat atgaatgtgc agatggtaat tggcaatttt
     5821 ttaagataat tgtcggaaca attatccaaa aatttggtta tccagttaaa gaatcagcac
     5881 tgaaagaagc atttgataat atagaaaatt gttatcttca aaatggctgg tatcgagaca
     5941 gttcaagagg tcgccaagat tattataatc cttttgcatt tcattattat ggtttgattt
     6001 atagcgtact agaaccagaa aagcccatta gccagatata taagaaccga gctattgatt
     6061 tttctagaga ctatattcat ttttttgcgg aagatggtgc aaatgtgcct tttggtcgaa
     6121 gcatgattta tcgttttgca gtttcaagct tttggtcagc tatgttgtgg gccgatcttt
     6181 tgccttttaa attaggtgaa atcaaggggt tagtcaatcg gaatataaga tggtggatgc
     6241 aaaaagaaat atttgatgaa aaaggtatcc tgaatattgg ttatacctac cctcaattga
     6301 cattgactga accctataat agtacgttat ctccattatg gctgaataag atttttttat
     6361 tgttggcgct acctgaagac catgagtatt ggacggctaa agaaatgcct atgcctattc
     6421 gtgaaggaac taaacttctt tcagaagcca atttgctcat acaacatgat aatggacata
     6481 catttttttt gaatgcggga caattagggc caaatttcca tacattgacc aatgaaaaat
     6541 atttaaaatt tgcttattct agtcaatttg gtttttcgat accgcgagcg aatcaattga
     6601 aagcagaaat ggcaatggat tcaatgctag ggattcagcg agcggacaca tatatcacga
     6661 ccagttttaa gaatgagagc atcaaaacct atggacagtt catcgttcga aataatgtac
     6721 gagacattgt atgtaagaaa gaggtacttg cctctacatg ggaacctact ttacaggcaa
     6781 aaatcagaac ttggcttatt ccttttgaag gctggcagat tcgcgtacac aaagtagaat
     6841 tagatacaga atatgttcta tatgagacag ggtttgctat tgaatgttct cctgagaaag
     6901 aaggaataat catagaagaa gacaaggaaa actatagggt gagtgaagct ggcttttcag
     6961 gtattatctg tttaagtgac gatactatta aacgaaaatc cacagctatt atgggtttgc
     7021 ccaatacaaa cttgatgaca tgggaaaaca cttttattcc tggattagag ggtacatttg
     7081 gaaaaggaac ccattggctc ggaacaggag tggttgctca tcaagatcgg gattatagtt
     7141 tagaagtatg gaacaatcgt cccaaaattg attttgatgg gacaaccggg ctaactatcc
     7201 aaaataaaaa caataaaaaa agactaaaat tatgctaaag gctggtgggg aaatgcttga
     7261 taaagtgatg tttgaagaga taatacatcc agaaaaatgg ataaatgaat gttataaaga
     7321 attaatggaa aaagaatgga ataatgagat cgagcgttgt cgcagacaca cggagaggtt
     7381 aatcaaacgt atcagtaaca gtcagtatca attcccaagc gagtgtccta taaaaggacg
     7441 ttatccatta agagacaata ttggttggac agaaggattt tggacaggaa ttaattggtt
     7501 gttatttgag aagacgggtg atcctttatt tcaacaaaca gctgaaaaag cagatataga
     7561 ttttatccac aggattgact gtcaaattat cactaatcat cacgatcttg gttttttgta
     7621 ttctttatca accgtagcag attataaaat aacgggaaat cgaaaaatga aggaagcttc
     7681 aattgatgct gcagaaacat tacttcagcg tttttgggag aaggcaggag ttatacaagc
     7741 ttggggagat cctagtgatc cttcacaaca aggtagaatg atcatggatt gttgcatgaa
     7801 tcttccatta ttgtattggg cgagtgaaga aactggaaat caaaaatata aaaaagctgc
     7861 tcaggctcat attcaaaatg cagcagatta tttagtaaga gaagatggtt caacttatca
     7921 tacattttat atggatgtca aagatggaaa accaagatat ggaagtacgc atcaaggtta
     7981 tagtgataat tcctgttggg ctagaggcca agcatggggg atttacggtt ttgcattaag
     8041 ttatcgttat ttgaaagaac aaaaatattt tgatttaagc aaaatattag ccaattattt
     8101 tatgaatcgg ttgccagaag ataccatttg ttattgggat ttgatctttg atgattctag
     8161 cagagaagaa aaagatacat ctagtatggt gatcatggtt tgtggattat tagaattaat
     8221 agaatcaggt ctattagaca aaacagaaaa gaacttatac cagtctgctg tttggaaaat
     8281 tatgcgacaa ttgaaagaaa gatatgctac aacggaagaa gaagatggct ttttaacaca
     8341 gtctgtttat aataagccca aaaataatgg ggtaaataca ccttgtattt ggggtgatta
     8401 ctattattta gaagctttag tgagaatgac tagtaattgg tcttcttatt ggtgaaaaag
     8461 gtaaatatgg aatttttgtt tgataaaata aaaaaagaaa aaccaaaaca gcatgaatgg
     8521 tttctgtttt ggttttttcg ttaattttca tattcttcta attccaagct tagttcttcc
     8581 cattcc
//
LOCUS       DASJON010000124         8530 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJON010000124 DASJON010000000
VERSION     DASJON010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.125"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            145..804
                     /locus_tag="U3989_002044"
     CDS             145..804
                     /locus_tag="U3989_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1715484.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            889..1794
                     /locus_tag="U3989_002045"
     CDS             889..1794
                     /locus_tag="U3989_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1715485.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1796..2428)
                     /locus_tag="U3989_002046"
     CDS             complement(1796..2428)
                     /locus_tag="U3989_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1715486.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2579..2704
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2748..3272
                     /gene="infC"
                     /locus_tag="U3989_002047"
     CDS             2748..3272
                     /gene="infC"
                     /locus_tag="U3989_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN1715487.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3344..3544
                     /gene="rpmI"
                     /locus_tag="U3989_002048"
     CDS             3344..3544
                     /gene="rpmI"
                     /locus_tag="U3989_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN1715488.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3597..3956
                     /gene="rplT"
                     /locus_tag="U3989_002049"
     CDS             3597..3956
                     /gene="rplT"
                     /locus_tag="U3989_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN1715489.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4208..5545
                     /locus_tag="U3989_002050"
     CDS             4208..5545
                     /locus_tag="U3989_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN1715490.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5593..7722
                     /locus_tag="U3989_002051"
     CDS             5593..7722
                     /locus_tag="U3989_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN1715491.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            7697..8386
                     /locus_tag="U3989_002052"
     CDS             7697..8386
                     /locus_tag="U3989_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715492.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         2756 a         1503 c         1841 g         2430 t
ORIGIN      
        1 aaaacacttt tttcttaaga attttttgac tttttttctt ctttgactag aaaaaaatac
       61 aattgacact actagatatt gggggtatat ttaaaaaggt tggagttaaa cacaatattt
      121 agtgatgaag tgagctggag gtaaatgagc gtgatcgttt tagcaggtac aattggtgcc
      181 ggaaaatcaa gtttaacaga aatgatggct gaacactttg atagtcaggc attttatgaa
      241 tcgattgatg ataatgaagt cttgccctta ttttatgcaa accctgaaca gtatgctttt
      301 ttgctgcaaa tttatttttt gaataaacgt tttgcaagca tcaaacaagc aatgaaagat
      361 gacaataatg tattggaccg ttcgatttat gaagattcac tgcttttcca tctaaatgca
      421 gatctaggaa gagcaacaga aacagaagtt cgtgtgtatg atgaactgct ggaaaacatg
      481 atggaagaac ttccctatgc tgcccataaa aaacatccgg atttattagt tcatattcgt
      541 gtatcttttg atacgatgtt ggagcgtatc gaaaaacgtg gtcgttcgta tgaacaattg
      601 tcctttgatc caagtctata cgattattac aaagaattga acagacgtta cgatcaatgg
      661 tatgaagaat acaaagaaag tccaaaaatc caaatcgatg gagaccgcta taattttgtg
      721 gaagatcctc aagcaaaaga agaagtcttg aaaatgatcg aagaaaaatt agcagagatt
      781 cgccaaacca aagtagccat ttgatttaca gttgctcaaa taaagggtaa acctttattt
      841 gagcgacttt tttctttata ctggaaacaa atgtcaggag gatagagtat gcgggaaaaa
      901 cactatttta atcaagaccg taatttttgg atcaatgtac agcaaaacga tgtgctagct
      961 gttaaaaact taaaagaaga atatggcatt agtgacgaga tgctgactta ttctctagat
     1021 aaaaatgaac gggcgcgcgt agagtacgat tcttttgatg aagcgttact gcttgtatca
     1081 aatgttccgc accagcaaaa agtcgataat cattatgaaa ctagtcctat cgcttttatt
     1141 ttaaaagaag acggtttgtt tacatttacc acgccgaata ccgagtacgt gattcgacta
     1201 attcgatcgc ttttggaacg aatgccagac atgagtgtat acagtttatt gtttcgtaca
     1261 ttgtttttga tttctgattc cttttttcct ttaatcgagg aagtgaatag tgaacgtcag
     1321 cggcttaatc tgaaattaag agaaaaaaca acgaacaaga atctgctgca actttcagat
     1381 ttagagatcg gtttggttta cttagttaca ggaacaaaac aaaatgtagt acttttagaa
     1441 caaatcaagg cattggctat ttatcgcaag ttgtcagaaa aggaaaaaga agagttggac
     1501 gatgcgctga tcgaagcacg acaagcagtt gaaatgacga acttagcttc gcagatttta
     1561 gaccagctct ctggcactta taataatttg ctgaataaca acttgaatga cacgatgaaa
     1621 tttttgacgg tctggtcgct gatcttgacc gttccaacta ttgtgacagg attcttcgga
     1681 atgaatctcc agttaccctt tacccattcc gttttcggat ggggaatcgc cttgatcatc
     1741 agtttggtct tatctatttg gatgcttatt gcactgtggc ggcggattcg ttgatctagt
     1801 ctttgatctg caaaccaagg gcttgggcga tcacaagtgc ttgctcttgg ttgacgatta
     1861 gtcccgccaa agaatcttga gataaagcga ttgctgtgaa gaaattagtc gtaaagtcta
     1921 atcccttcag agaagttcga aaccagttcg aaccggtcaa ctgattgtca gacaaaaaaa
     1981 ggtttttcca agtgacctca taaaattcgc tgtcttctaa ttgacaatga tcgaaggaga
     2041 cagctttcaa gtttgtacta ctaaaggaag ccatcgaagc aatacaatca gtgaaagtac
     2101 agtctcgaag ataactttcg gcaaaattcg tacctgttaa tttacattga tgaaacacga
     2161 cttggtggaa actgccacca atccactcca gattggaaaa atcacatttt tcaaaaatga
     2221 cattactgca ttcaaaatga cgtaaaactg ttttttgcat cgtaagtttt tggatatgac
     2281 tctctcttaa taccatattt ttggcatcga gatagctttg gtcacccccg ttgatcagaa
     2341 gaccagagaa gatctcctca tcttctaaat aaaactctcc ctttgtaaga agaggaagag
     2401 aaggtggaat aggttcgatt tttttcatgt gggttcacct cgtttttctt tagtatagca
     2461 aagaagcggg aatcactgca agaaagtctg ctaatctaat cattttcaga aaaggaagac
     2521 aaactgtatg aaaaacattg acaaaaagat tagtttttgc tatactatga tggttgagaa
     2581 ataaaagcag aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt
     2641 tcactggttc tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg
     2701 ccgtttattc ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa
     2761 aggatatgat ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg
     2821 gtgagcaatt aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact
     2881 tggatcttgt gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg
     2941 gtaaataccg tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga
     3001 tcaatgtaaa agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac
     3061 ttcgtaatgc acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca
     3121 aaggccgtgc cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag
     3181 aaactgcgga tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct
     3241 tgacgctggc accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag
     3301 ccgatagtcg gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac
     3361 acaccgcgga tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg
     3421 cgcgtttaca agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa
     3481 agcaagcatg gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa
     3541 ataagaagta caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg
     3601 cacgtgttaa aggtggaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa
     3661 aaggttacta cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt
     3721 catacaacta cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga
     3781 ttgctcgtat caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg
     3841 gtttgaaagt tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg
     3901 atgcagcagc atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta
     3961 aacaattata aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt
     4021 acaatcctcc atcaaatgaa acaggacaat aacgtataag tctaaaatag cataattaag
     4081 gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc taactacata
     4141 agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag
     4201 gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt
     4261 ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg ctactgtatg
     4321 gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg aagggcaagg
     4381 agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg ccaaatcgat
     4441 cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata gcggaatcgc
     4501 agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa tcacgatgag
     4561 cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg gaccagtgat
     4621 ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag taccggttgc
     4681 actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg tcaatttcaa
     4741 acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata cttataatga
     4801 ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac taacggtcgc
     4861 taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac cagctgaagg
     4921 tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag gggtcgttct
     4981 tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat tgctgtttac
     5041 tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat tatttacaga
     5101 tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga tgttcaataa
     5161 ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga taccgcaatc
     5221 accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt tccgcggtcc
     5281 aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg ttaatccaac
     5341 gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc ctcagcattt
     5401 agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa cttctcgaga
     5461 atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat gcctgatcac
     5521 gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat tagatgaatg
     5581 gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg tacacatacg
     5641 aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc agtgaaaaca
     5701 acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca aaattgtttg
     5761 ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac cacacaagcg
     5821 actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat ggcaaaaggt
     5881 ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga tttagggaat
     5941 aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa tgtagaccgt
     6001 gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt ttcttctatg
     6061 gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc atcggtaaaa
     6121 tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac aagaagaact
     6181 cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc aacctctaca
     6241 gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg cggagacggc
     6301 ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct ttccggtcca
     6361 gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt ttattttgaa
     6421 gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc aatcgattat
     6481 tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt tctaggtgtg
     6541 gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg tccacgttct
     6601 gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat caaaggtcct
     6661 aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa ggtagtgatg
     6721 gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct tggattagtg
     6781 caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat ccaagcatta
     6841 gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga aaaatcctat
     6901 gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga gaaagatcag
     6961 atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt tagtaataaa
     7021 atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat tggtgtagca
     7081 cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa agaagatata
     7141 cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac accagaatcg
     7201 atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat cgctactggt
     7261 tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga agcactagag
     7321 cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga aaatacacct
     7381 ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct tcggatcatc
     7441 gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa aacaactgca
     7501 gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta tcaaactgaa
     7561 ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga ttttacagcg
     7621 acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat ggaaccggag
     7681 gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag agttactaaa
     7741 cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc gagatttatt
     7801 ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa tcgattgtgg
     7861 tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag gagaaatttg
     7921 tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg aacgaatcac
     7981 ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt tagaaaaatt
     8041 aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga ttattggaca
     8101 tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa gcgaaaaaat
     8161 tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat tgagcgagat
     8221 agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt atgtattaga
     8281 ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag aagaaataaa
     8341 aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag actgtgacaa
     8401 aagtcgtgtc acagtccttt ttttccgaat aaacgatgtt ctcctgtcaa ctcctcggtc
     8461 acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
     8521 ggagatgaac
//
LOCUS       DASJON010000125         8484 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJON010000125 DASJON010000000
VERSION     DASJON010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.126"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..163)
                     /locus_tag="U3989_002053"
     CDS             complement(<1..163)
                     /locus_tag="U3989_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715493.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFT"
     gene            complement(583..1704)
                     /locus_tag="U3989_002054"
     CDS             complement(583..1704)
                     /locus_tag="U3989_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN1715494.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2032..3441
                     /gene="istA"
                     /locus_tag="U3989_002055"
     CDS             2032..3441
                     /gene="istA"
                     /locus_tag="U3989_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN1715495.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3438..4166
                     /gene="istB"
                     /locus_tag="U3989_002056"
     CDS             3438..4166
                     /gene="istB"
                     /locus_tag="U3989_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEN1715496.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4294..5205)
                     /locus_tag="U3989_002057"
     CDS             complement(4294..5205)
                     /locus_tag="U3989_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1715497.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5222..6229)
                     /locus_tag="U3989_002058"
     CDS             complement(5222..6229)
                     /locus_tag="U3989_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1715498.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6226..8355)
                     /locus_tag="U3989_002059"
     CDS             complement(6226..8355)
                     /locus_tag="U3989_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1715499.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8360..>8484)
                     /locus_tag="U3989_002060"
     CDS             complement(8360..>8484)
                     /locus_tag="U3989_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1715500.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2594 a         1726 c         1484 g         2680 t
ORIGIN      
        1 tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta tatcctcgtt
       61 tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc ataccagttt
      121 ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt ccttgatggc
      181 tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta tacaacaaca
      241 gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat gctcactgat
      301 gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg tccggaaagc
      361 ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga aaaatctatt
      421 tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt tcttgctaaa
      481 aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa attttagtat
      541 gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag gtttgtaacg
      601 tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt tcaattgaaa
      661 cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg ttgcgattaa
      721 ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc gtttaatggt
      781 cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt gatacatgtc
      841 ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga ttttttgtcc
      901 cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac cgccgacaag
      961 aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg ctacttcaat
     1021 ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga aatagccgtc
     1081 acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca catacgttgc
     1141 ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg catttgtcat
     1201 cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca aatgtagctt
     1261 gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt tcaacggaat
     1321 agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct gagcagaaat
     1381 tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt ctggcgctct
     1441 acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga ggcgctgatc
     1501 taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg catgaagtaa
     1561 cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt cgctaattaa
     1621 gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt tttgaaatga
     1681 ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg ataagtcatc
     1741 cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc ttttgatggg
     1801 ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa agatatttta
     1861 ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa tttaactaca
     1921 cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact ggtttccttc
     1981 catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac tttggatgat
     2041 attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca gataagaaaa
     2101 gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca actattcatt
     2161 acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga aatcatcggt
     2221 agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc caaagctatc
     2281 catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg tgtatacatt
     2341 tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga tcgtcttgat
     2401 catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca tgaagatcac
     2461 atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac gtccgcaagt
     2521 gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact atttaatcaa
     2581 tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt gaaaaaaaca
     2641 aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac cattcattat
     2701 ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca tgtcgaaaat
     2761 aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga ctcttttgag
     2821 tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac gcattattca
     2881 aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca ttctcttcca
     2941 caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta tcacgaaatg
     3001 aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca attgactgtt
     3061 gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt aatgaacgaa
     3121 tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat gaaacaatgg
     3181 agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc tgcacgcctt
     3241 caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa acaattacta
     3301 agtttattag cgacctacga tatccaacaa attaatgaac aattttacga attagtcgtt
     3361 caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc attaacaaaa
     3421 acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa aagcttcgat
     3481 taagccatat catcacgaac ataccaagag cttctttcga agctccagaa caatttatta
     3541 ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa cggatggaaa
     3601 aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac gaagatattt
     3661 ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta aaaaggcaag
     3721 aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg atcgcattag
     3781 ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac aatctcgtag
     3841 agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag cgattcgaaa
     3901 caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa gaaggagctg
     3961 aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt attacctcta
     4021 atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca gctgctctcg
     4081 ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt ttcagattga
     4141 aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa ctctgcactt
     4201 tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac gacgtaattc
     4261 caaatgttgg attgctttta gataatcttt tatttattca caattgctaa tagtccaatt
     4321 ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg acaactcatc
     4381 aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt aaataggatt
     4441 cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt tttcaacata
     4501 atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct ctaaaaactc
     4561 ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt gtcttacttt
     4621 atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa tatgattgcc
     4681 tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg tctttttccc
     4741 ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt gtttttcaac
     4801 atctagaatt tttactgata caacctcgga actagtggga atatccgctc gaaccatatc
     4861 ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca ttctcttttc
     4921 aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta cagtaaaatt
     4981 ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat gagttgtatg
     5041 ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag acaataaaag
     5101 ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt ctttcttctt
     5161 ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc attcctcttt
     5221 tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta tcggtaagat
     5281 tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca taaatgccca
     5341 catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa tcgcctggtt
     5401 tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc acccgaggga
     5461 gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca aacccagttc
     5521 cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc tcgtcaaaaa
     5581 ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc actaataagc
     5641 aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc gcgatgggat
     5701 tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg gctaaagcaa
     5761 aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca tagttataag
     5821 cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc aaagacgaaa
     5881 aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt gataaacctg
     5941 ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact tgaataatcg
     6001 ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt tccaccattg
     6061 aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt ccagaactac
     6121 cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg cccacaaaag
     6181 agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc ttttctcacc
     6241 tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg ttcgctttgg
     6301 aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg gctgagtgct
     6361 tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat tcgaatgtac
     6421 ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg gttttatctc
     6481 cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt cgttcaatac
     6541 tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc gcaactcccc
     6601 tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt ttttcgtctt
     6661 ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta cttgattttt
     6721 tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc caacttttct
     6781 accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt gttttttcag
     6841 atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg acactctatt
     6901 gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac caactgctgc
     6961 accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca attttcgatt
     7021 catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat cattactttg
     7081 aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa aggtcacaat
     7141 ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa acatcgtgga
     7201 gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa tcgtattgaa
     7261 caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg acaataagaa
     7321 actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca acataatccc
     7381 tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa agaccacggt
     7441 tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat cttcaatctc
     7501 tgctttgact gcttcttccc gatccttacc cttattttca tccggactca cggataaaat
     7561 cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt gtaatagtaa
     7621 ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca cactatcttt
     7681 tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag ccgcttcact
     7741 aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg caatgaaaga
     7801 acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt tggtagtttc
     7861 tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca tcaataatag
     7921 gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg ctaaagtttg
     7981 gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt aagcttcttg
     8041 aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa aattggtaat
     8101 ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc aaggtaacca
     8161 atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat atttggaata
     8221 aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta ccgtaccgac
     8281 taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca caatccgaaa
     8341 aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga gtgtcaaaag
     8401 catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc ccatacaagt
     8461 cttgcatcaa gcattgaccg tttt
//
LOCUS       DASJON010000126         8320 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJON010000126 DASJON010000000
VERSION     DASJON010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.127"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..3064
                     /locus_tag="U3989_002061"
     CDS             <1..3064
                     /locus_tag="U3989_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN1715501.1"
                     /translation="RLTEAPLFLISGKTGAGKTTIFDGITFALFGETSGRLRSGKEMR
                     SLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKRKGNGTKKQAAKVTLTIFDGKGK
                     EIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGEFRNFLVSSSSDKETVLRHLFGT
                     QFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFVLVKEEEPAAGFEAVLAQWTNHQ
                     AILEKQITEEKEALTELQEKQKQLENSYYRFESLKKSYEEKTILINKQKELAAQKDEI
                     EEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQKSSEQTKKLAMTTQNYEEWQKNE
                     ANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQDAEQLTISLDKEELNAEKLYQNL
                     AIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEKQQEIEKQKAKDTGSLAQIREEL
                     KKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARLQLFLRPGEPCLVCGSTEHPIQQ
                     LKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSEAHENNLQQQLSDLEQKIMATNI
                     QLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSLDRRKSKMANARIELEEIRQQAE
                     KLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQDTDSNCLEEEIRRLEKQLEEDSN
                     QLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEKIEKEQERIAKKLESQSYFSTIE
                     EICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQLENIQLTETFPDTEEISQQLEEI
                     RRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQTKLDELTQLQQLSQTFNGENPK
                     KTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFELADTIGSYRGKTGLEINIYDDQA
                     GMSRSAHTLSGGESFIAALSLALALADVIQTQAGGVAIDALFIDEGFGSLDEEALEMA
                     MEALETIESEGRMIGIISHVRELKERVLQQIRIDTEGSGQSKIRYQLG"
     gene            3083..6277
                     /locus_tag="U3989_002062"
     CDS             3083..6277
                     /locus_tag="U3989_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN1715502.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(6423..7130)
                     /locus_tag="U3989_002063"
     CDS             complement(6423..7130)
                     /locus_tag="U3989_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1715503.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            7895..8293
                     /gene="spxA"
                     /locus_tag="U3989_002064"
     CDS             7895..8293
                     /gene="spxA"
                     /locus_tag="U3989_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1715504.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         3026 a         1418 c         1678 g         2198 t
ORIGIN      
        1 acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac
       61 gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg
      121 aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt
      181 tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg
      241 aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa
      301 aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact
      361 gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg
      421 taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac
      481 tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa
      541 attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga
      601 agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat
      661 actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa
      721 gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac
      781 gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa
      841 acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca
      901 agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact
      961 agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg
     1021 acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat
     1081 tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa
     1141 agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg
     1201 aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg
     1261 acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac
     1321 tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa
     1381 tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa
     1441 gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg
     1501 atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt
     1561 aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat
     1621 tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa
     1681 aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa
     1741 aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca
     1801 atctttagat agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag
     1861 acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa
     1921 aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga
     1981 tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga
     2041 ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac
     2101 gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga
     2161 aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga
     2221 agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac
     2281 ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca
     2341 gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg
     2401 tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca
     2461 aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct
     2521 tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt
     2581 ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct
     2641 ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg
     2701 agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc
     2761 gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc
     2821 cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg
     2881 atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga
     2941 aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt
     3001 acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg
     3061 ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg
     3121 acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt
     3181 gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa
     3241 agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt
     3301 tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc
     3361 aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg
     3421 tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga
     3481 aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat
     3541 cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca
     3601 agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa
     3661 acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac
     3721 gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct
     3781 gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt
     3841 gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc
     3901 tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt
     3961 tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac
     4021 gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc
     4081 agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct
     4141 gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc
     4201 cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt
     4261 cgtctatggt gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc
     4321 ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta
     4381 tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata
     4441 tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag
     4501 aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga
     4561 gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga
     4621 tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt
     4681 tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga
     4741 atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca
     4801 aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga
     4861 tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag
     4921 agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg
     4981 tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc
     5041 ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta
     5101 caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg
     5161 gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta
     5221 tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct
     5281 tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt
     5341 agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt
     5401 gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga
     5461 acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca
     5521 aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga
     5581 agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat
     5641 ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg
     5701 acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct
     5761 cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt
     5821 ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc
     5881 gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt
     5941 taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa
     6001 tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga
     6061 acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat
     6121 gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact
     6181 gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat
     6241 ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta
     6301 atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga
     6361 ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa
     6421 ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata
     6481 tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag
     6541 ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct
     6601 tctggaagca tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg
     6661 aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt
     6721 tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca
     6781 ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca
     6841 agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag
     6901 tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct
     6961 cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga
     7021 tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc
     7081 tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac
     7141 gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt
     7201 ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa
     7261 ggtttttata tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga
     7321 acaaattaat cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt
     7381 ttcgaaagat ttattttttt aatacagtct caatataact attcaaacat aaaataacgt
     7441 tatttgattt atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg
     7501 cttatataac agagaaaata agcgttataa taaagaacag tattatttaa aatgacagaa
     7561 aacattacta ttaaagaaat aaaaaacata aaaaatctta agaaatcggt ttcatttagc
     7621 ttttgagata aaataatagt acagttttat acgctgtttc atatgtttag aaaatttgaa
     7681 ttagatttga aaactctaat ttttaaatga taaaaaaagc gagaatgctg gttttactgt
     7741 atattaacct tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt
     7801 aaaagttgat tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa
     7861 aagtgattct tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag
     7921 ttgtacttcc tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga
     7981 acgtaatatt ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac
     8041 agaagacgga acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat
     8101 ggatttagat gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt
     8161 gcttcgccgt ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga
     8221 aattcgtcgg ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat
     8281 ggctggatta tagaaggata atacgaaact tggaaaagac
//
LOCUS       DASJON010000127         8249 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJON010000127 DASJON010000000
VERSION     DASJON010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.128"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            234..1559
                     /locus_tag="U3989_002065"
     CDS             234..1559
                     /locus_tag="U3989_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1715505.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="U3989_002066"
     CDS             1552..1902
                     /locus_tag="U3989_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715506.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1884..2069
                     /locus_tag="U3989_002067"
     CDS             1884..2069
                     /locus_tag="U3989_002067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715507.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /locus_tag="U3989_002068"
     CDS             complement(2973..3248)
                     /locus_tag="U3989_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN1715508.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="U3989_002069"
     CDS             complement(3220..4173)
                     /locus_tag="U3989_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1715509.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="U3989_002070"
     CDS             4785..6278
                     /locus_tag="U3989_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1715510.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="U3989_002071"
     CDS             6413..6709
                     /locus_tag="U3989_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN1715511.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="U3989_002072"
     CDS             6810..7661
                     /locus_tag="U3989_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715512.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7965
                     /locus_tag="U3989_002073"
                     /pseudo
     CDS             7682..7965
                     /locus_tag="U3989_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2863 a         1283 c         1553 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgacg
//
LOCUS       DASJON010000128         8241 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJON010000128 DASJON010000000
VERSION     DASJON010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.129"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..732)
                     /locus_tag="U3989_002074"
     CDS             complement(31..732)
                     /locus_tag="U3989_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1715513.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(748..1188)
                     /locus_tag="U3989_002075"
     CDS             complement(748..1188)
                     /locus_tag="U3989_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1715514.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1188..1763)
                     /locus_tag="U3989_002076"
     CDS             complement(1188..1763)
                     /locus_tag="U3989_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN1715515.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(2611..3090)
                     /locus_tag="U3989_002077"
                     /pseudo
     CDS             complement(2611..3090)
                     /locus_tag="U3989_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(3249..3950)
                     /locus_tag="U3989_002078"
     CDS             complement(3249..3950)
                     /locus_tag="U3989_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1715516.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(4058..5467)
                     /locus_tag="U3989_002079"
     CDS             complement(4058..5467)
                     /locus_tag="U3989_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN1715517.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(5515..7380)
                     /locus_tag="U3989_002080"
     CDS             complement(5515..7380)
                     /locus_tag="U3989_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1715518.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(7501..>8241)
                     /locus_tag="U3989_002081"
     CDS             complement(7501..>8241)
                     /locus_tag="U3989_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1715519.1"
                     /translation="EISPEAVQKEFILRDSFAKNQFQQLMADIPAEEIEWVKQVVELA
                     EDKLKVEFSPNIYLTLTDHLHYAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLF
                     VKQKTGVELSEDEAGFIAFHFVNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDE
                     ESLNYQRIITHLQFFAQRYLKGEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQ
                     KQEMGIAEQIYLTIHIQRVVSEKKSIQS"
BASE COUNT         2349 a         1822 c         1370 g         2700 t
ORIGIN      
        1 ttttctttcc cgtcgtttgg ttttttattt ttagcctaat tcttgacctt ttgcatgctg
       61 gttatccgta aaatattttc cagcaggctt tccttccaat tcagctaatt tttcttggat
      121 ctgacggatc accttttttg catcttctgg attttctaca aaatcgaatt tatccccatc
      181 cacttgtatt ttaggacata catcaaaatc ttcataccat tgattatagc gggtgttcaa
      241 cgtttcataa taggtatata acgtctcatc gctttctagc tgttcatagt cacgcccacg
      301 ttttttgata cgttctagca tcgtgtccaa agaaactttg atatggacca tcaaatcagg
      361 ccgttttttt ggagcaacat catctaactc gtttagcatc gtatctaata agctatcgta
      421 ctgctgtact tcgatatccg ttacccgacc taaatcagcg tttagatgga acaacaggct
      481 atcttcatag atggatcggt ctagcacatt gtcatcgttc actaaagcat ttttcattgc
      541 taaaaatcgc ttgttcaaaa agaagatttg caataaaaaa gcgtactgct caggatttga
      601 ataaaagagc ggcaatactt cgttatcttc gacattttca taaaatggtt tagaatcaat
      661 ctcttttgca agcatatccg tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa
      721 aatcactgac atacaaccct tcctcttcta taagtactcg cctacgtaag tttcaacagg
      781 caatgtttca aaatcatatt cacgtacagc ttggtctgat tttaaatacg tatggagact
      841 ttgtgtaatc atcaagttcg taggtaccgt ctcttcttgt acaatgatca tcggtttttt
      901 caacatagtt gccacaccta attcgtaagc cgttcctgga tcgattgttt gatgctcaaa
      961 gtcaaggata gcaacaacaa attctgcatt tgtcagttct tccatatctt tttcgtatac
     1021 ttcctgcgcc catccagcgc tgaatagttc ataattgctt tcttggtgat gacgtggaga
     1081 gtagaaactt gtgactgttt tgttttcctc caacgctttt tcgatacgac ttactcgatc
     1141 gatttgctcc tctgaaaaaa atggtcctgc taaatatatt tgactcatta ttttgcctcc
     1201 ttcaataata actgattttt aatagatact gctcgtaatt cattgaccag ttccaatgga
     1261 ttgttggaaa agattacttg atccaaccat tttttatcca taaaatcctc ttttgcaaaa
     1321 gtttctaata attgttgcaa gccgctgtaa taaccgccaa tattcaatat tccgattggc
     1381 ttgtcgggcg tcagaccgat tgctatccaa gaaagaagtt gtgagaattc ctccaatgta
     1441 cctagacctc cagggaaaac gaggacagca tctccctttt caaataataa tacctttctt
     1501 tcatcgatcg tttctgtagg aataaatgta gtaacatctt ttccaggcaa ttcagtttca
     1561 aaaagcccat tcggatagat acctgtgact tttccgttat tttccagtac tgttttacta
     1621 atcactccca taatcccgga gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc
     1681 atgtattttc ctaacgtttg cgcaataaat ttgtaacttt ctttattacc aaaacgggaa
     1741 ccgcaaaaaa cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt
     1801 ctacacgtgt ttttttaaaa taaaagacta gattttgtag ttttttagtg gtatatagga
     1861 tagacgccac tatatatagt atttagaaaa aaatcatttt tcagccattt tatagataaa
     1921 acaaaaataa ataacgtgtt ttcatcgata tgaaatgact tacttataga agatactggg
     1981 atttcgtcct atatgccaat aacgtgtatt attttaaaca acaaatactt gtttctttta
     2041 ttaacgaaaa aatcattttg ttctcgtgtt atgtttcact gataaatgat gtttctctaa
     2101 ataaaaaagc cgacgaacta aacgatcaaa atttgatcat ttttgttcat cagcttctcc
     2161 taaaaatctt tttgttaacg ataaatagct ggatttgtaa aaatatcact tgaatcgtca
     2221 ctgctggaag aaaattcagt gataaccgca cctgttgcgc ctgcctggaa ctagtgtttg
     2281 atattgggct cgaacgtgta ctgatctcta gcatgaagaa taatttcttt ttttactgta
     2341 taataacttt ctttcctttt tggaatagta caggaaagtt tttttgtttc ctttccttcg
     2401 acataaggta taccagaccc tttcgacaac gaaaggttct catttttcct tcatcgtcat
     2461 ttcaaggagg gtgccgatgc tctggacagt ttcgatcgct cctaaaccaa aatctgtata
     2521 ttcgattctt tctaactctg attctttcat accattcctg tctcaaggta catctattgt
     2581 acttttttct tgtaatccat tctgcctctc ctaactaatt gtcaaatcat cccaagatat
     2641 gtcttcttcc tctttttctt cttggatgat ttctgcattc ggatctactg gttttttcaa
     2701 taataaaatg gttattcctg tgacaaaaga accagctaac aatgcaccaa tgaatgccca
     2761 tggtctggtc atcgtagcga ctgcaaacat tcccgatgcc ggaatacggc tatctgcgcc
     2821 ccaaaacatg cttaatccgc cacctactgt cccaccgact cccgttgcta tgacattgcg
     2881 tgccaagtcg ttcaatacga tagggaaaca tccctctgtg atctgacaga tccccatagg
     2941 aaaagcggtc ttcaatgtat cgatttcttg acgattgagt atatttttac gaatgatttt
     3001 ccctaagaaa taagcgatcg tcaagccgaa tggggttacc atcgaagcta aaatcaatac
     3061 agtgattggt tcatagattc cttctgaaaa tcgtgccaca cccccttatc cagaataaat
     3121 ggtaatccag aaacagccgt gtatctgcgg taataagcct aaccaaacga aaaatatgag
     3181 aagctatttt tgtttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
     3241 ttttttattt atgtttcata atcaatatgg taaggatgca gctccgtatg gtagaccgcc
     3301 tccgaatatt caatcatttc cccaatttgc gtagacgatt tccgtatccg tttgatgccg
     3361 atctgtgcgt ctgttttcag aattccttgt tcttcttctg ttaaaaatac tgccgagaaa
     3421 tcatcagaaa atgagtcaat agtatatcct tttttcgtca gcaaacgata caaagattct
     3481 ttgccaaatt cttctctgct gaattgcatc atttcttgtg gaagataatg aacaaaataa
     3541 atataaggct gattgttcaa ataatataag cgcttaaaac aaacagcttg ctcaccgaaa
     3601 aaggaaaaca aaggatcttc cttctttata tggaccaatt gcaaatccag cgtttctttc
     3661 cggacttcat atcgggagtt ctccaagatt tgcgtaaagc ttgccgcttt ggataatcga
     3721 ttgtaaggac gattgcttag aaccgtcgtc ccacgaccac ttttctttgc gacatattca
     3781 tctgatgcca gcagttcgat tgcctttcgg acagtgattt tgctgacttg aaacagttct
     3841 tctaattcac tttctgtcgg caacatcgaa ccgactggat attttcctga gaaaatatct
     3901 tctttaattt tatctgcgac ttccaagtat agtacacgtg tccttttcat aacgtcaccc
     3961 tttgctttac tattactaaa agaatagcac ataaaaaata aaaaaagaac acaaatcaca
     4021 tgtgagaagt gtgttctttt tcattcagct gctttaatta caagtttacg ccatttgagg
     4081 caattacttc tttataccaa tcaaaagatt ttttctttga tcgattcagt gtcccatttc
     4141 cttcatcgtc tagatccaca tagataaagc cgtaacgttt actcatttct cctgttgatg
     4201 cactgacaag atcgatacat ccccaaggcg tatagcccat caaatctaca ccgtctgcta
     4261 ctgcattagc catcgcttcg atatgacgac gtaaataatc gatccgataa tcatcaataa
     4321 tcgtgccatc ctcttccata tgatcaatgg ctccaagacc gttttcaaca atgaataatg
     4381 gtttctggta acgattgtat aattcattca atgcgattct cagcccttct ggatcgatct
     4441 gccagcccca ttcacttgcc tcaagaaacg gattttttac tcctcccagc aagttccctt
     4501 ctgaagttgc tgcttgtgga tcagtctcat tgattgccgt agacatataa taactaaatc
     4561 cgatgtagtc cactggatat tcttttagca actggaagtc tttttccgtt tgctctaatt
     4621 tttccggatc gactccatat tttttatggg tacgcgctgt gtatgcaggg tattctccac
     4681 gtacttggac atccgtgcag aaaaaattga attcttggtt ttggatcatt gttgctgcct
     4741 gattgactgg attggcatcg atgctgtaag ttgttgcata gataatcatg cagccgattt
     4801 ggatatccga acgcaattcg tggccgattt ttacggcttt gctacttgca acgaattgat
     4861 tatgccatgc ttggaaaata ttttggtact ctcctgctcc attgcttttt actaatcctt
     4921 gacttaaagc tggaaagtga aaggccgaat tgatttcatt gaaagtcatc caatatttga
     4981 ctttactgct gtatctctct aatactgtct gagcaaatcg ttcatagaag tcgatcagtt
     5041 ttcgattttt ccagccaccg tattcttttg ccaaatgaag aggcatttcg taatgagaaa
     5101 tcgtgatcac aggttcaata tcatatttta agcactcatc aatcagctga tcataaaatt
     5161 ctaatcccgc ttcgttagga gtaccttcgt ctccatttgg aaaaattcgt gtccaggcaa
     5221 tcgaaaaacg ataacatttg aatcccattt ccgcaaacaa ggcaagatct tctttgaaac
     5281 gatcgtaatg atcgattcct cgatgattcg ggtaaatata tttttcttga tcaatcgtcc
     5341 agtcaaattc ttcactgccg ataatcgcaa agcgctgctt acctccaggc atagcatctg
     5401 caacggatag tccttttccg tctcgatcat aagccccttc taattgattt ggcagcagtt
     5461 gctccacccc ataaaaattt ttctgaaaaa cgtgattgca tcctgatttc ctccttattt
     5521 tacaattgcc agaacatctt cgtttgcaat aatctctttt tggtctaatt caagtatatc
     5581 ggcaaatgca tcagtattcg taatgacaac tggagtgatt acgctatagc cagctgctct
     5641 aattgcttcg atgtcaaaac gaatcaataa atctcctgct ttgacgagat ccccttgttt
     5701 gactaagatc tcaaagccat tcccatttaa ttctactgta tccatcccga tatgaatcaa
     5761 aatctccgct ccgtcagtcg ttgtgatccc tacagcatgt ccagttggaa aaatagtcgt
     5821 gatctctccg tctgcaggtg caactagttc tcctacttct ggatcaatag cgataccttt
     5881 tcccatcacg ccagaagaaa aaacttggtc tggtacttca ctcaatggca gtactttacc
     5941 cgttaaagga ctttttaagg tatgccgtgc tgaagtaatc ggttctcctg cattcgcatg
     6001 tttgttttct aattgattgt cttgtgtttg ttcgtcaaat cctaaaatca atgtacctac
     6061 gaatgcaaga acaaaagcaa ttcctgtagc tattactgct actgtcacat ttgactctac
     6121 accatccacc gtattaatga aagtcggaat cgtcaataag ctgaccaaac tgctggagaa
     6181 agcttttact ccggaaaatc cgataatagc tccaccaata cctccagaaa tacaagcggc
     6241 aatgaatggt ttcttcaaag gtaatgtcac accatacaca gttggttcag tgattccaaa
     6301 taacgaagtc actgtagaag aaatccctaa tgctcgaagt ttcgtatctt ttgttcgcaa
     6361 tgctacagct aaagcagccc caccttgtgc caagatggct ggaagaagca ttggcatcaa
     6421 tacatcaaac ccatattgtt cgatattcaa aaacatgatc ggtacgaatc cccaatgcat
     6481 gccgaacatc acgaatactt gccataaact tcccatgatt agacctgcta cgattggact
     6541 aaatccataa atcgagttga agagaccgcc taaagcatta ccggcaaccg tcccgattgg
     6601 tcctaaagca agaaatgtta atggaaccat cactactaaa acgacaagcg gaactaaaat
     6661 catttgcaga aattgcggaa tcactttttt tacgaatggc tctaatttac tttgaaccca
     6721 tacggctaga atgattggta tgacagaaga agtataacca gatggtgaaa gaatcactgg
     6781 gatcccaaag aaactgatag cttctcccgc accagcaact gctgtgatag ctggatacac
     6841 aagtgccata gcaattgcta ctgctaaaaa ctggttggct ttgaactttt tagctgctgt
     6901 aaaagcaagc atgacaggta agaaggtaaa gacaccatct gctgcagcaa acaatatttg
     6961 ataagtaccg ctatcagctg tcagccagcc catcaccgtt gccaatgata agaatccttt
     7021 caataccccg gcggctgcca tcgcccctag aaaaggtgta aatatactag agataatatc
     7081 gatcagacga ttcaaaatat ttttggactc ttcttttcca tcctccgaaa ctgcaagacc
     7141 ttcctcatcc acgatcgatt gataaacatc tgctacatta ctgccaatca ctacttgata
     7201 ctgaccgcca ctttgtacga cttggatcac gtccgggtct tcttttaact tttgcgtatc
     7261 tgctaaagat tcatctttta atctgaatcg caaccgcgta gcacaatgta ctaaactttt
     7321 gacattcttt tgtcctccta ccgcttccca tacacgacga ccaactgctt gattatccat
     7381 tgttttttcc ttctttcttt tttatttaaa aaataaaaac ccagattgaa aaaaacagtc
     7441 acaaacattc gtgaacctat tttttcaatc caggttttgc ctgccataca gtaacaatcc
     7501 ttatgattgg atgctttttt tctcacttac tactctttga atatgaatcg ttaaataaat
     7561 ctgctctgcg atccccattt cctgtttttg cgtttgtcga agatactcat tgatccgttc
     7621 gacacaatga aacgcttttg gatactttcc ttgaactaat gcgtagagaa attcatcttg
     7681 ctcgtcagat ccttcccctt tgagataacg ctgggcaaaa aactgcagat gagtaatgat
     7741 acgctgataa ttcaaagatt cttcatcgaa cacgatgccg aagaatttac tgatgatact
     7801 cagtatatct cttaccattg tggtagcggt catcgtcact tgcatgtctt cgtttccttg
     7861 ttggctattc acaaaatgaa aggcaataaa accggcttca tcctcagaaa gttccacacc
     7921 cgtcttttgt ttcacgaaca aaagagcctc tttcgctgct tgatattctt tcggataaaa
     7981 tttttttgtt tcaaataata atggattagg cagttggata ttttcttttg ctcgtgtgat
     8041 cgcataatga agatgatcgg tcaatgtcag ataaatatta ggcgaaaatt caacttttag
     8101 tttatcttca gccagttcga ctacttgttt gacccattcg atctcctctg ccggaatatc
     8161 agccatcagc tgctggaatt ggtttttagc aaaagaatct cttaaaataa attctttttg
     8221 gaccgcttct ggcgaaatct c
//
LOCUS       DASJON010000129         8206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJON010000129 DASJON010000000
VERSION     DASJON010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.130"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..446
                     /locus_tag="U3989_002082"
     CDS             <1..446
                     /locus_tag="U3989_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1715520.1"
                     /translation="KSVRAYSFQKIGTKKNSGKFGKWDPSKFTVEDAIFGTLELENGK
                     IIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFEDVDGKLVSLQKTTPVNDPHEL
                     SMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAETGEVITL"
     gene            443..2626
                     /locus_tag="U3989_002083"
     CDS             443..2626
                     /locus_tag="U3989_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1715521.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            2626..3754
                     /locus_tag="U3989_002084"
                     /pseudo
     CDS             2626..3754
                     /locus_tag="U3989_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            3751..4428
                     /gene="pgmB"
                     /locus_tag="U3989_002085"
     CDS             3751..4428
                     /gene="pgmB"
                     /locus_tag="U3989_002085"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1715522.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            4512..5039
                     /gene="msrA"
                     /locus_tag="U3989_002086"
     CDS             4512..5039
                     /gene="msrA"
                     /locus_tag="U3989_002086"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1715523.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5240..5926)
                     /locus_tag="U3989_002087"
     CDS             complement(5240..5926)
                     /locus_tag="U3989_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN1715524.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            6006..7544
                     /locus_tag="U3989_002088"
     CDS             6006..7544
                     /locus_tag="U3989_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN1715525.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            7664..8131
                     /locus_tag="U3989_002089"
     CDS             7664..8131
                     /locus_tag="U3989_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN1715526.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
BASE COUNT         2703 a         1570 c         1814 g         2119 t
ORIGIN      
        1 taaaaagcgt tcgggcgtac agcttccaaa aaattggtac gaaaaaaaat tcagggaaat
       61 tcgggaaatg ggatccaagc aaattcacag tagaagatgc aatttttggc acactggaat
      121 tagaaaacgg caagatcatc caactgaata cttcgtttgc tttgaatatc agagaagaca
      181 aactgaatgt ggctttttgt ggcgatcagg caggtgctaa tctgtatcca ggcgaactat
      241 tcgaagatgt agatggaaag ctggtttcct tacaaaagac cacgccagtt aatgacccac
      301 atgaactaag tatggctcat tttgtgaatc gggcgctcgg tgaccagact cagatgattg
      361 cagatggctg gcaaggatat gtcatccaga atctggtgga aaatttatat cgttcggcag
      421 aaacaggaga agttattacc ttatgaaagt agaagaaaaa cagtttgatt tagagtatat
      481 caataagtat gcgagtcaga tgaccattgg aaatggattt tttggtatca gaggaagcca
      541 ggaggaagac tatcggaatc aagtaagggg aatgtttgca gctggtgtat ataaccgacc
      601 tttaggagca gaatcagccg agttagtgaa tctcccagat gtcatcagat gggaaataaa
      661 aatcgacggt gaacattttt ctcttgagac gatgaaagtt cttgcatacg agcgggcact
      721 agatttagaa atgggagagc ttaaacgcta tgcgctcatt gaaactgctc aaggaaaaaa
      781 agtggaagtc attacttgtc gtgttgctga tcaaaataat ctccatcagg cagccctgaa
      841 agtggtcatc aagccgttga ccgaagactg ccaagtagat gtgaaaacgg gtatcgatgg
      901 acagcaaacc aattttggta cacagcagtt ggttgaaaaa gaactgcgag tatttggtga
      961 cgatttgatc agtgcatcct accagacaac agaaagtaaa attgatattg ggattgcagc
     1021 taaattcaat aaagcaggtg tattccaagc aaaaaatcgc cggatcaccg cttccttttc
     1081 agaaaagatc agaaaaaata cgtcctttac gctggagaag atcatcgtgc tctctacctc
     1141 tcttcagcaa acagacccgt ttaagaaaag tattgcccaa gcccagaaaa gcgttagtta
     1201 tacagaaatt ttagagcagt cgatgtctta ttggacagat ttttggcatc agcatcggat
     1261 aacgatccaa ggcgattctt ctttcgatca gttagcgatg gacttcgctg cttaccattt
     1321 acagattatg actccccgca atgatccaag tgttagtgta ggggcgaagg gactaactgg
     1381 cgaaggttat aaagggcatg ttttctggga tacggaaatt tttatcctgc ctttcttttt
     1441 atatcagttt cctgaagtag caaagaattt gctgctgtac cgttatgatc gattagaagg
     1501 cgcacggcaa aaagcaagag attatcacta tcaaggtgca cttttcccat gggaaagcgc
     1561 acacactggt caggaagaga cacccaaatt tgctgcattg aatatcaaaa ccggaacaag
     1621 acaagaagtt gcttcagctg ccgcagaaca tcatatcagt gcagaccttg cttatgccgt
     1681 tcaagagtat gtccaagcaa cagaggatca atcgttttta gaaacaaagg gaaaaacgct
     1741 cattcatgaa acagcagctt tttggctgag cagagctgta aaagtcgatg atcatttaga
     1801 gatacacaga gtcattggtc cagatgaata cacagaatat atcgacaata atgcatatac
     1861 gaactatctg gcgcattata atgtggcctt agctgttaaa tatcaagctg gcgatccgct
     1921 ttttttgaaa gaatgtcaaa attttttaga taagttatat ttgccagtgc ctaacaaaga
     1981 acagctgatt ccgcaagatg acacgtatct cagtaagcca gttattgatt tgaccactta
     2041 caaagaacgt cctggcagcc agtctatttt acttgattat gcccgtagcg aagtaaatga
     2101 actccaagtc agcaagcaag cagatcttgt catgctgtta tacttattac ctgagtattt
     2161 ttctcaacca atcgtttcta agaatttgca ttactatgaa aacaaaacaa tccacgactc
     2221 atcgttgagt aaagcgattc acgccataga ggcttgccgt atccaagaaa tggaatgggc
     2281 gtatcattta ttccaagaag cgtgcctgat tgatttagga ccagatccgc attcatcaga
     2341 tgacggaatt catgctgctt cattaggagc actatggcta gcagttatat ttggtttttc
     2401 aggtattaca gtaacgaatg gaactctttc tatttctcca agacttcctt cagcatggga
     2461 aaaaatttct tttcctttta tttggaaaaa tcacaggttg ctatttgttt tgacaaaaaa
     2521 acaaatagta ataaaaaaag agacgaaaga aacattacct atttttgtca atggagacca
     2581 ataccagctg gaaaaagaac tgatcttgga acggagggga aaataatgcg tattctgatt
     2641 gcgccagatt ctttcaaaga aagtctgaca gccttagagg cagcacaagc cattcaatca
     2701 ggttttcaag aagtttttgt tcaggctgaa tatgatctgc tacctatagg cgatggagga
     2761 gaaggaacgg tttcatcatt agcagacagt ttaaggttga agcggcaaac agcagtcgtt
     2821 actaatgcat ttggcaaaaa caaagaaatc cagtacgctt cagacggtca aactgcgatt
     2881 tttgaaatgg cagaaatcgt cggattgaaa gatgtcccac taaaccaaca acagccactt
     2941 aggttatcca cgcgaggagt aggagaactg atacgacaat tgttgcaaca aggtgttcga
     3001 cacatcatca tcggcgttgg cggaagtagt accaacgatg gcggcatcgg catggcagaa
     3061 ggtctaggtt atgcttttta cgatcaagat aatcaaaaag tagaagcact gggagaaaat
     3121 ctaggtaaaa ttgctacagt tgataaaacg aacgtgccta ttgagctgat taaagccaaa
     3181 atcactgtca ttactgatgt tactaatctt ttatgcggaa aagaaggcgc ttcttttgtt
     3241 ttcggaccac aaaaaggctt gaaagaaaat gaattagcag tggctgacca agccatgcat
     3301 gatttttata tgagatttgc ccctcaagta atcgaggcat cgggcagtag tgctggtggt
     3361 ggaatgggtg caggactgtt agcatttgct tcagggaaaa tagtctctgg catcgactac
     3421 gtattggatt tactggagtt cgatacgggt acagcaagca gacctcgtaa tcgtaggcga
     3481 aggacgtcta gatggacaaa gtctaaaagg aaaagcgcca gttgggattg ctagaagagt
     3541 accagagggc gttcctgtga tagctatttg cggaagtgtt ggtaaaggaa gcgaaaaagc
     3601 agcagatgtt ggcatttcgg ccgtttttcc gatccttcct gcagctgtgc ctttacaaga
     3661 agctttacag caagccactg ataatcttca tcgcacagct aaaaacatcg catctttact
     3721 tcaagtcgtt cattttcaag gaggtaatcg atgaaaatca aagcagcact attcgattta
     3781 gatggcgttc ttgtagacac agctcgttat cattacgaag cgtggctggt tttagctaac
     3841 cagctgtcca ttccattcac tgagaaagaa aacgagcaac tgaaaggaat atccagaacc
     3901 gagtcactag agcgattgct gtcatttgga aaaatggaac agaaattctc agaaaaagag
     3961 aaatcagcct ttgctgagca aaaaaacaat ctatatcttc aagcgattca aaaaatggat
     4021 gaaacatccg ttttgcctgg tgcaatagct gttcttgagt acttgaaaaa aacaaacatc
     4081 aagatcggat tgggatcagc aagcaaaaat gcacgcctca ttttagaaaa aacgaatttg
     4141 acaagttatt ttgatgtcct tattgacggc acgcaagtat caaaagcaaa acctgatcca
     4201 gaagtttttc tcaaaggagc acagcaattg aatgtaccgc caaatgcatg cttggtcatt
     4261 gaagattcag aagctggttg tcaagcagct ttggctggta acatgcatgt attgggaata
     4321 ggtgaaaaca taaatcttcc ttctgctgaa tatgtgattc ctgatttaac cgtttttgat
     4381 caagtacgtt cgttttggca tttatcagaa gccgtatcac gtatataata gtttccgttt
     4441 tttctgtcac aagcaaaacg ttatcctcct tttggaaatc aaggtatgat gagaaaaaag
     4501 ggggatttta tatgacaaaa aaagcagttt tcgcaggcgg atgcttttgg tgtatggtca
     4561 aaccatttga agagcagcca gggatcattt ctgtgacatc cggttatact ggcgggcatg
     4621 taccaaatcc aacttatgaa caagtaacaa caggcacaac agggcataca gaagcagtag
     4681 agatcgaata tgatccagaa gttatttctt atgatcagtt ggtggaaatc tattggcagc
     4741 aaaccgatcc gactgacgca ttaggacagt tcgctgatcg cggtgattct tatcgtccag
     4801 tgatctacta taatgatgaa gaagaacgaa atattgcaga gcgttcaaaa caagcattgc
     4861 aagacagcgg gcgattcgat aagccaatcg tgacaaaaat cgaaccaaga agcgaatttt
     4921 atcctgcaga agattaccat caggattatt acaagaaaaa caagctccat tacaatcttt
     4981 atcgagaagg ttctggacgt gcaggtttcc ttcgtcgaca ttggggcgtc aagaaataag
     5041 aagccgaaaa agctacttat ggaacaccgt atatcagagg ttgtaaaacc agcattttga
     5101 gctaatactg agggtcagct gattgaacac catttagtgg agaggttgtg aacctgtcgc
     5161 ctagctccaa gtcacaagga tttggtaaga gaacactata tatcagaggt tgtgacaagt
     5221 cgtttgtcac aacctttttt caatacctaa gatgaaggac aatcagcaac gcactgctca
     5281 aaaaaggaat gaaaggcagc ttttccaact tttttttatg acagataaag taaacagaaa
     5341 aaacggatat tccaaacaga ctagcaagaa acaagatctg aagcaactct tctagagaca
     5401 agccaccact ccaagaaagc atcaacagag tatcgccagc tccgagatag gattgaccat
     5461 agcgaaggca cacaccgcct acgataaacg ttaacagcac actttgccat tggaagacgc
     5521 cggcctgcaa ccaaaaaatc cataaaattg cccaagaaca gaatagcgtg tgagaatgga
     5581 ccatgaagta aaataaatcc atcaacgaaa atagcaaagc cgaaagcatc catatgaacg
     5641 tcgaaaaatt tcttgcggaa gaaaaagtaa agaaccaggc aaaaagacca ccacataagg
     5701 cttctgccaa caaatagtag aagggcaacc gacacctgca atagcggcag cgaaatcgtt
     5761 ggacgatgat cgagagaatc ggaatcaatt cataccaaga aagcgaacga cagcatttga
     5821 cacaatgaga tctaggataa ataatagagt gacctaacgg aatccgctgg gcggttaaac
     5881 ataaaaatga tccgatacag caaccaatga taaaataaag taacacgcca tctccccctt
     5941 ttcaaaacca agatacgcaa gcttaaacta aatttttgta catacataaa atttgaggtg
     6001 aaaaaatgaa cgtttcaatc ggattagata tcggaaccac acaaaccaaa gcagttgctt
     6061 tcacagatga agcccaagaa gcagcatccg cttattttcg ctaccctttg atccaagaaa
     6121 cacaagggat ggcagaacaa gatcaggaat tgatctttca aggtgtctgc accgttataa
     6181 aagaagtaat taaacagttg ccttcagaca caatcattca atttgtctcc ttctcctctg
     6241 cgatgcatgg cctgatattg ttggatgaag agaaaacacc gttatctcga atgatcactt
     6301 gggcagataa ccgagcagcg gacgaagcag aaaaactgaa atataccgtg cttggcaaag
     6361 aattttatcg gcagactggg acgccgattc atccaatgac accattgaac aaaatcaagt
     6421 ggctgcaaaa cagccaacct gaattattca aaaaaactgc ttattttgtc ggtatcaaag
     6481 actatatctt ttatagattg tttggcgaac tgatcagcga ctacggaaca gcttcaggaa
     6541 ctggatattt tgatatccat gaattccaat gggcagaaga agtgcttgac tacttgaaca
     6601 ttcgaataga gcaattgccg caagtctttc cttccaccta tcaagtaaca ggtctaccat
     6661 caaaaacagc agcagaacta ggtttgccaa atgatatatc atttgttttg ggaggggcag
     6721 acgggccgct ttctaatcta gggttaggtg cttttggtga tacgatcgcc gctttgaccg
     6781 taggaacgag cggagcactt cgttttatta ctactcagcc tcagttccat ccagaaatgg
     6841 agacgttttg ttatgttctg gatcagaaac attgggtagt gggaggagcg actagcaatg
     6901 gtgctggcat ctttgattgg gcagggcata catttttaca ggaagtcgtt caatctgcta
     6961 aagaaaaagg agaaaatcca tatgatgcac tgctttcggc tatcgctttt gtgccagcgg
     7021 gtgccaatgg tctgattttt catccctatt tacttggtga gcgagcaccg ctttgggacg
     7081 cagatgcgtt tggcagtttt atcggtttac agcggcaaca tacagaaaaa gaaatgatga
     7141 aggcgattct agaagggatc tgtctcaacc tttatcggat tttaaaagga gtttgccagg
     7201 aaaatcagct cacagagatc cgtgctacag gcgggtttgc tcaatctccg atttttcgtc
     7261 agatgatggc tgatgtactg ggttacccgc ttgttttccc tactgtcaca gaagcatccg
     7321 cattaggagc tgtgatttta ggctggagaa gcatcggaaa gatccaaagt atcagtgaag
     7381 cacaggaatt gatagaattg aaagagcaag ttggagtaca agaaactgat cataaaaaat
     7441 atcagaaaat ctatccatta tttgtttcga cacaaaagca tttagcacag acttatcagc
     7501 aatttgctca actgcgagaa gaattagctg attctgaaaa atgattggct ttacggataa
     7561 aaaagcgaac tgaaaatcgt tctcaggtaa atgattttgt tgtacttttc attgaaagtc
     7621 tactattatt ttaagtgtaa gaaaatgtta ggaggcaaga aagatgaaat tccaacaaac
     7681 caaagaagta ttaaatcaat tagttgcaga tctaagccaa atgtctgttg ttgttcacca
     7741 aacacattgg tacatgagag gaccaggttt ccttacattg cacccaatga tggatgagtt
     7801 catggatgat ttgaacgctc agttagatga agtatccgaa cgcttgatca cattagacgg
     7861 ttcaccttat tctacattgc gtgaatttgc agataacaca aaaatccctg atgaaattgg
     7921 ccgctgggat cgtactattc cagaacgttt ggaaacactt gttgcaggct atcgttatct
     7981 agcagatctt taccaaaaag gaatcgaagt ttctggagaa gaaggcgacg atccaacaca
     8041 agatatcttt atcgaattca aaacagccac agaaaaacgt atctggatga tccaagctca
     8101 tttgggcaaa gcgccagaga tcgacgcgta aaaactgttg ttgaacttga ccgaaaaaga
     8161 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacgt
//
LOCUS       DASJON010000130         8058 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJON010000130 DASJON010000000
VERSION     DASJON010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.131"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(6..527)
                     /locus_tag="U3989_002090"
     CDS             complement(6..527)
                     /locus_tag="U3989_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1715527.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            726..4418
                     /gene="nifJ"
                     /locus_tag="U3989_002091"
     CDS             726..4418
                     /gene="nifJ"
                     /locus_tag="U3989_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN1715528.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4607..5494
                     /locus_tag="U3989_002092"
     CDS             4607..5494
                     /locus_tag="U3989_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN1715529.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5491..6660
                     /locus_tag="U3989_002093"
     CDS             5491..6660
                     /locus_tag="U3989_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715530.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6684..8039
                     /locus_tag="U3989_002094"
     CDS             6684..8039
                     /locus_tag="U3989_002094"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN1715531.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2590 a         1485 c         1828 g         2155 t
ORIGIN      
        1 tttttttatt ttgtttggat caattctttt aattcctcta ttgaaataga ttcatcaaag
       61 atttctttac ccaagatgac agttggaaca aatcgaatat gtgcttgctc agcttcgttg
      121 atgacagctt ggctcagctg ttcgtctttt tgttctgtca gctttagttt atctactgca
      181 aaatccgcta cttcttggag agaaagatgt ttccactcgt cttgagtatc gaatatttgt
      241 ttgatctctt tgatcgcttt ttggccatcg ctgattgtga gataccggtg catcacgttc
      301 cctcgctgta agctttcttt ttctttatcg aacaatttga tcactcgctg caattgcccg
      361 ctttggactg cttcttctaa tagctcataa gattcttcaa accattgtcg gcaatacgga
      421 caagctaagt tgatgaattc gaccattttt ttaggagcat tcgatgaacc ataaaggatt
      481 cctttttgtg tatttgtttt cgttgcatca atcactgata tatccatgtt aaaacacctc
      541 tttcgcttat agtttataca tgtctgacaa aaaaaggcaa cgatttagac tgtttgtcta
      601 aaaattagat gaccaattca aatagataca tgtaaaatat ttttataaat aaaagaaaaa
      661 aatggcatgt tttacgttat aatggaacag ataataagag agaatatgaa caaggatgat
      721 gaatcatgcg aaaaatgaaa acaatggatg gcaatacggc agccgcatat atttcttatg
      781 catttaccga gattgctgca atatatccaa taacacccag ttcgaccatg gctgaattag
      841 tagatcaatg gtcagcagca ggaaaagaaa acgtattcgg tcaaccagta aaagtagtag
      901 aaatgcaatc cgaagcagga gcagctggtg tggtccatgg atcattgaaa actggtgcac
      961 tgacaactac atataccgct tcacaagggc tgcttttgat gataccgaat atgtacaaga
     1021 ttgctgggga actactgcca agtgttttcc acgttgctag tcgggcagtg acgacaaatg
     1081 cgttgaatat tttcggagat cacggagacg tgatggctgc aagacaaaca ggatttgcta
     1141 tgttagctga aagcagtgta caagaagtga tggatttagc acctgttgcc catcttgctt
     1201 ctattgaagg agaagtccct tttttgaatt tctttgatgg attccgtacg agtcacgaga
     1261 ttcaaaaaat cgaagttttg gaatatgaag aattagctcc tctgatcaat caggagaaat
     1321 tagcttcatt tcgtaaacga gggatgaatc ccaatcatcc gactgttagt ggtacgaacc
     1381 agaatccaga catccatttt caacaacggg aaacgatcaa cccctattat gataaattac
     1441 ctgaaatcgt tcaaaaatat atgaaagaga tcaaccgatt acgaggaact tcttatgact
     1501 tggtgactta ttacggagca gacgatgcgg aagaagtcat tgtttctatg ggatcagttg
     1561 cacagacgat tgaacaaacg attgattact tgaatgcaaa cggacggaaa acaggttttt
     1621 tgaatatcca tctttatcgt ccttttccag tggaaacatt cgtgcaaaag ttgcctcata
     1681 cagtaaaaac aattgctgtt cttgatcgaa gtaaagaacc aggggcaggt ggagaacctc
     1741 tcttattaga cgttcaaagt gcgttatatg attctgaact tcgcccggct gttattggtg
     1801 gtcgctacgg attggggtct aaagatgtga ctcccgacca aatctcagca gtttttgatg
     1861 aattgaaaaa agacccaagc atacgcaaaa aacggtttac catagggatc gtggatgatg
     1921 tgacttatca atctttggaa ccaaaggaaa gtctggattt gacagaaccg caaacattcc
     1981 aggccaagtt ttggggattt ggatctgacg gaacagttgg agcaaataaa tcagctatca
     2041 agattattgg agatcacacc gataaatatg cgcaaggcta tttttattat gattcgaaaa
     2101 aatctggcgg tctgactgtc tctcatcttc gttttggaga tacgccgata cgttcagcgt
     2161 atcttgtcga gcatgcggac cttgttgcct gtcacacacc agcttacctg catagctatg
     2221 acttggtcaa aggattgaag ccaggtggca tttttttatt gaatactctt tggagcgacg
     2281 aacaattaga aacgcatctg cccttaaaat taaaacgtta cttggctgaa aataacatta
     2341 gattctatac gatcaatgcc atgagattgg cccaagaagt cggtttggga cgccggatca
     2401 atacggcaat ggaaacggct ttttttaaat tagctgacat cattcctttt gatgaggtgc
     2461 ttccgctgct aaaagaagaa gccctgaaaa gctatggaca taaatcaatg gctatcgtag
     2521 aaaaaaacat acaagcaatc gatcggacga ccgaactgct tcatcaagtc gcgatcccag
     2581 accattggag aacgttggag atgcctgaaa aaatcaataa aaggaacact ggttatatac
     2641 aagaaatact tgaaccagtg aatgcacagg aaggaaatca actctctgtt ggtacacttg
     2701 tagcaaatgg aatgacaact ggcgaaatgc ctttaggaac ggcttctatg gaaaagcggg
     2761 gcgtcgcttt agaagtacct gagtggatta gtgatcgctg tacgatgtgt aatgaatgtg
     2821 cctttgtttg tcctcatgct gccatccgtc catttttagc agatgaagag gaaatggaag
     2881 aagcgccaga aggatttatc gtgagagaaa tgcgaggcgc agacggcgta aaataccgta
     2941 tccaagtttc agtggaagac tgtacaggtt gtggtctgtg tgtagaggct tgtcccgcaa
     3001 aagaaaaagc tttagtgatg aagccttatg aagaacagaa agaacaagct gtgaattggg
     3061 cttttgcaat gacattaaga caaaaagcaa atccagtcaa aggaaagaat actgtcttgt
     3121 ctacacaatt caatcaaccg cttcttgaat tctcgggcgc ttgctcaggc tgtggcgaaa
     3181 caccttatgt aaaattattg acacaaatgt tcggtgatcg aatgatgatc gcgaatgcga
     3241 caggctgttc ttctatttgg ggcggtgctt ctcctgcttc tccttatacg accaatgaat
     3301 gtggtcaagg gcctgcatgg tcaaattctt tattggaaga taatgctgaa tttggttatg
     3361 gtatgcttct agcggcacag actcgtcgcg aggctttggc aagttcaatc aaagaagtat
     3421 tgccaaaagt atcagaagaa ctacgagaat tgatggaaga ctggttagta cacatgtttg
     3481 agagtgaagg aacgcagcaa agagcagcaa aactgcaagc tgcgatcgaa agcgaacgat
     3541 caattttccc tgaactggag gatatataca agagaaagga tctattcgta aaaaacagcc
     3601 aatggatgat tggtggagac ggatgggctt acgacattgg ttttggcggg atcgatcatg
     3661 tacttgccag cggagcagac gtgaatatcc ttgttttgga taacgaagtc tattccaata
     3721 ctggcggtca aacctcaaaa gcaacaccag cttcggcaat cgctaaattt tcggctagtg
     3781 gaaagtatgc ttcaaagaaa gatctaggaa tgatggcgat gacatatgga aatgtttatg
     3841 tggcgcaaat tgcttcaggt gccaatcaaa tgcaaacgat caaggcattt gaagaagcag
     3901 aaaagtatcc tggtccttct ctgattattg cctatacgcc ttgtatcaca catggcttgc
     3961 ttggcggaat gaaagaatcg cttaaagaag caaaagaagc tgtttcttct ggttattggt
     4021 cattatatcg atataaccct acaatggtcg aaaaaaacaa gaacccaatg actttggact
     4081 acaaaaaacc tgatttctct gctatgcctg atttcatgag gaaacaagtt cgcttttcct
     4141 cgctagataa cgcaaatcca aatcttgcaa aagctttatt tgaaaagact gtccacgacg
     4201 ctaaaacacg tttttacaac tatgctgcac tgacgggaga attagaaaaa atcaagaaac
     4261 gacttgaacc agaggaatct atcgatgtac ccgttaagac tagaaaagaa aaaacgacgg
     4321 accttgaagc agaagaaaga agagctgcta gaagagctgc cagagcagaa cgacgtaaaa
     4381 aaatggaaca agctacaaaa gaaaaaaagg aaaaataaca gaaaaaatat aatgaataat
     4441 tctctgaagt ttaaaaatat ttattgcaga gaaaaataaa acgcatactt gcaattttct
     4501 tgaaaattat gcgtttttct caagaaaaat acgagcatat cagcctctaa aattaaaaca
     4561 tatgatatac tagagaggaa atctcaaaag agaagagagg ctttttatgt catttcaaat
     4621 cggggaactt tttaatttgg actattggca gcaaatgttt tccggtgact tgttctctct
     4681 gaattttttg gtgaacatac ttgatattct ggttgtctgg tatcttgtat acaaactgat
     4741 ccaactgctg cgaggcacaa aagcgattca gcttctgaaa ggtgtagcta tttttattgt
     4801 tatccgaatc gtagcggaaa tcattggtct gcacaccttg tcttggctga tgaaccaagt
     4861 cattacgtac ggggtgatag ctgctatcgt gatttttcag ccggaaatcc ggagaggact
     4921 ggaacatctt ggacgcagtt cgcttttcaa acaatcaaaa agcgaacagc aggaagatga
     4981 gaagatgatc ttgtcttttg acaaagccat ccaatatatg tccaagcgaa aaatcggtgc
     5041 gttgatcact atcgaacgtc atactggatt agatgagtat attgagacag ggattgcttt
     5101 agatgcagat atcactgggg agctgctgat caatatcttt attcctaata caccgctgca
     5161 tgacggtgca gttattgtaa aagaaggaaa gatcgcagta gcaagcgcct atcttcctct
     5221 atcagaaagt atgttgatcc ctaaagaatt cgggactcga caccgggcag ctgtcggtat
     5281 aagcgaagtc agtgacgcga ttaccatcgt tgtctctgaa gaaaccgggg acgtcagcat
     5341 cacgttagat aatgaactga tggctggctt atcccagcaa gaatatttgg caatcttgcg
     5401 tagagaacta attgctgaag agaaaacaaa aaacaagaaa aacgtgattc aatctttcct
     5461 agacggtatg actaagggag gggatcgcaa atgatctcaa aagagaaacg gacaagcatt
     5521 ctttacagtt ttattgcatt gctgttcagt attgcgctgt acttcaatgc aaatggccag
     5581 tccgttccaa atacgctttc tggaaatgaa gcgtataatc aaacagttac gggggtgccg
     5641 atcaagattt cttatgattc aaagcgctac tatatccatg gatatgaaaa tgcagtgact
     5701 gtcaaacttt ccagtgcaaa ccgagttcag ttgaatgcag aggcaaacga agatacacgg
     5761 atgttccgcg tcacagcaga tatcactaag ctgggcgaag gaacacatga agttccgcta
     5821 aaaattcaaa atttgagcag tgctgtcaca gcaaagatcg aaccatcgac tattacagta
     5881 acggttgaga aaaaggtaat aaaagatttc gatgtggaaa cgttgattcc ttcaacgttc
     5941 acaccatctg gatatgaact tgatgatact tccgttagtc ctaaaaaagt ctctatcact
     6001 acaggtgaca aaacattggc agaaatcaaa cgagtagtag ctgatgtgga cgcttcgatg
     6061 gtcacagatg atgggattaa tagtgaggtc ccaatacagg cgctgaatgc agcaggggaa
     6121 gtattaagta tcgtctctga tccagtgcaa gtaacagtga gagctgattc gatcaagcca
     6181 agcaagtcgg tacgacttta tgggatacag caaggaacac cagctgccgg ggtagaaagc
     6241 tatgatttca gtttttcaaa actggaagca gaagtaagcg gctctagcga tttgcttgca
     6301 tcgatcggtg acagtattgc tgtaccgatc aatgtctcag gtatcaatca tcgaacaact
     6361 cgtaaaattg aaataccagt agaagagggg ttaagtatca agcccgaatc agttagtgtg
     6421 gagattacac ccgtgttgca aacgtcaagc agtagctctg cttcgacgag cagtcggaca
     6481 caagaaaata caagtacgag tagaagtatt tcatctgctg attcaggaac ggaaccatcg
     6541 acaacaacca tttcttccag ttcggaatct gggacaagca gctcgaccac aagtacagaa
     6601 gaaacatcag aaacaagcga atcaacacaa atgagcagaa caaattcctc agaaaattaa
     6661 gttaaaggag taattttaca actatgggta aatattttgg aacagacggc gtacgcggtg
     6721 aagccaacaa ggaacttacg ccagaacttg catttaagct gggacggtgc ggaggttatg
     6781 tcctaagcca gcatgaagaa gaaggaaaac gcccacgcgt gttagtaggg cgggatacaa
     6841 gaatctcagg tcaaatgtta gaagaagcgc tgatttcagg acttttatcg gtgggtatcg
     6901 aagtgttcca gctaggtgtg atctctacac caggtgttgc ttatctgaca cgtttgcaaa
     6961 aagcaagcgc aggggtcatg atttctgctt ctcataatcc agcacaagat aatggaatca
     7021 aatttttcgg tgcagatggt tttaaactag tagatgacca agagctagaa attgaagcat
     7081 tattagatgc tgaagtggat gaattgcctc gtccatcagc tgaaggattg ggaactgtag
     7141 aagaatttcc agaaggctta ttgaaatatt ctcaattcct tgtacaaacg attaacggtg
     7201 acctttccgg tttgaccatt tgtgtcgatg cagcaaacgg agcaacttca acaagtgtta
     7261 atcgtttgtt tgccgatctt gaaacagatt tttatacaat gggcacaaat ccagacgggt
     7321 taaatatcaa tgacggcgta ggctctaccc atccagaacg tttagctgag atggtcgttg
     7381 aaaaaggagc agatgctgga ctagctttcg atggcgatgg cgatcgtatt attgcggtgg
     7441 atgaacttgg aaacattgtc gacggtgata aaatcatgtt tatctgtgct aaatacttgg
     7501 cagaaaaaaa tcgattgaaa aaagatacga tcgttacgac tgtcatgagt aacttaggat
     7561 tccacaaagc ggtggaagga atcggtttga aagatgtcgt tacacaagtt ggtgatcgtt
     7621 acgttgtaga agaaatgcgc aaaaacgatt ataactttgg cggcgagcag tctgggcaca
     7681 tgattttctt agattttaac acaactggag atgggatgct ttcaggaatt cagttactga
     7741 atatcatgaa acagacaggt aagaaattat cagagcttgc agcagaggta acaatctatc
     7801 cgcaaaaatt agtgaatatc cgcgtaagtg ataaaaacgg tgcgatggat gttccggcaa
     7861 tcaaaaaagt gattgaagag gcagaagctg aaatgggatc agaaggaaga atcctagttc
     7921 gtccttccgg tacagaacca ttacttcgag tgatggcaga agcgcctact gatgagaaag
     7981 ttaattacta tgtcgataaa atcgcggcag tagtcaaaga agaaatcgga ctggattaag
     8041 agatattttc ataaaaaa
//
LOCUS       DASJON010000131         8009 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJON010000131 DASJON010000000
VERSION     DASJON010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.133"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(124..672)
                     /gene="aac(6')"
                     /locus_tag="U3989_002095"
     CDS             complement(124..672)
                     /gene="aac(6')"
                     /locus_tag="U3989_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1715532.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1225..2328
                     /locus_tag="U3989_002096"
     CDS             1225..2328
                     /locus_tag="U3989_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1715533.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2521..3588)
                     /gene="rlmN"
                     /locus_tag="U3989_002097"
     CDS             complement(2521..3588)
                     /gene="rlmN"
                     /locus_tag="U3989_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1715534.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3818..6265)
                     /locus_tag="U3989_002098"
     CDS             complement(3818..6265)
                     /locus_tag="U3989_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1715535.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6454..7890)
                     /locus_tag="U3989_002099"
     CDS             complement(6454..7890)
                     /locus_tag="U3989_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1715536.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2222 a         1739 c         1368 g         2680 t
ORIGIN      
        1 cacggtgttc caaaagtagc ttcttcggaa ataagttgga atttcacaaa aattcaagaa
       61 gtaattttcg taaattcctt cttatttctt gaagctgatt acttctgtca caacctcttt
      121 ttattattga gaatttggtc gaggaataat cgtttttgcc atccaaatat ccggtttgtc
      181 ccagccattt gcatttggta agacacctac gattttataa cctaattttt catagaattc
      241 atacggatgt tcacgaaggt tctggatgga agccacttta tcaaatgtat gctcatacag
      301 gtccgtttga cttaacgttg ttccatggtc taaatcatcc gtacctaaat aaatcgtgat
      361 tcctcctctg gaagctactt ctttttctaa gtaattgact aatcgagtac ctatttggtt
      421 ctttcgtcgg gagctttcta caactaatgg atgcaattcc caacctgtga taccgtattg
      481 agggattgca ccaataaatc ctactaactc atcttggtct accgctgcta ccgcgattcg
      541 ttctggattc atcatttctt ctacttcttc tgccgagctg tctccatatt cttccggcca
      601 agtcagtctc agtaaatcag aaagctgatc tttcaatact ggattattac ggtcaaattc
      661 actgattatc atacgtcttc ccccttgttc ttgcttattc taacagtcct tttgaaaaaa
      721 cgaaatgttc tcgctcaaaa atctttagct accaatcagt atctctattc tttcattatt
      781 tatcttactt ataaaataaa aaaattatac agaaacactt tattcaaaat aggaaagatc
      841 aggaaagcca tcaattggag gaaaaaactc tgtttgttca tcatgatata gattatcttt
      901 tgctttaaaa aaaaccaatt atgaattgac aaataaaacc tgttttcata taataatctt
      961 aatttttaga atttttagat aattacaaaa aagcaaaggg aatcggtaga aatactaaga
     1021 ggaacaaaaa ttcattattg tcgttttcaa tgtttttgta cttgttcgtt tttaggatac
     1081 ttattgatga tttcaaggtg ttcatgccac cttattcatc acttatcctg tcttaggttc
     1141 acctctactc catctacagc cgcagtcaga tcgatcactc tttaaaattt taacccgttt
     1201 caacgaagaa agaaggaaat gaatatgagt caaagtgttt tcttaccaaa ttataccatt
     1261 ggaacagatg cgtatgaaaa agcaaatgga atcgtcaaag aatatgggac aaaagtagtg
     1321 ctgattggag ggaagactgc actttcaaag gcagaaaaca aaataaaagt agctttgaca
     1381 caggaaatag aggttgtcgc cagtctctgg tacgggggta attcaactta tggaaatatt
     1441 gaacagttga tccagcatga aagtgtccaa aaagcagacg tcctgtttgc agtcggtggt
     1501 ggccgtgctt gcgatactgt aaaagtcatt ggtgaaaaac tagctaaacc agtcttcacc
     1561 tttcccacta ttggttcgaa ctgttcgccc gtcactgcgg tatgtgtcat ctataacgaa
     1621 gaaggcgtaa tggacggact cttctttcct tcaaaaccac ctgtacacac cttcattgat
     1681 acagcaatta ttgccgaagc acctgataaa tacttatggg caggtattgg cgatgcttta
     1741 tccaaagaag tcgaatctac tttttctgct cgtggcgatg atctaggcta cacggataga
     1801 ttaggaatcc aaatcggaaa aatgtgcatt gatcgtctct tgacttatgg tacgcaagca
     1861 ttgaaagata gtgccgacga tcgtccctca tatgcaattg aacaagtcgt ttcggatatc
     1921 attgttacta ccggattggt ttctgtttta gttaacaata actataattc tgctatggca
     1981 cactcattct attacggtac gactgttcta gaaaaaatgg agggatacct tcacggagca
     2041 tctgtcagtt atggtgtact gaccttattg atgatggatc agcagttcga tttgtttgat
     2101 aaaatctatg tgtttatgaa aaagaaccaa cttccagtat cgttaaccga catgaatctc
     2161 aatgtcacga ccgatttaga agcaatctta gataaagcca tggcaacaaa tgatattaaa
     2221 catgtgcctt atgaaatcac ccgagaaaaa tttaaacagg ccattcttga cgtagaaact
     2281 tatcatgcta cacaagaaag ccagcaagga tataagaaaa tcagttaaat aattgctaaa
     2341 acccccatca agcaggctgt catcaaattg aggacatttc tttgcttgat gagggttttt
     2401 cttgtcaaaa aaagctttat agctttcgct atagaatctt ttctcttatt taagttgaac
     2461 agttccttaa ttaagaaaac tgctcctttt atggtaattg aaaaaacgag taggatacga
     2521 ttatttggca actgtttttt tcatttgctt gcttcgcaat tgtccgcaag ctgcatcaat
     2581 atctgtacca tgttctttac gtatcacaca gtttacgccg tttttcttca atacatcata
     2641 aaatctcaat acatcagctt ttggactgcg agaatattga tcatgttcac tgactggatt
     2701 atatggaatc aagttgacat atgctaattt tttcttatct tttaatagat ctgctaattg
     2761 ttgcgcatgc tctggtctat catttacttg attcaacatg atatattcaa acgtgattcg
     2821 acggtttgtt ttctctaaat actcatccac agcttccatc aatttctcaa ttgggaaact
     2881 tcggttgatc cgcatgatcg atgttcgcac ctcattattc ggcgcgtgca aggaaatagc
     2941 cagattgact tgcaagccgt tattagcgaa ttccttgatt ttgtgtgcta atccactagt
     3001 ggaaactgta atgtgccgag caccaattgc caatcctttg gcatcattga tgatatgcaa
     3061 aaagtccatt acattgtcgt aattatcaaa tggctcaccg atccccatta caacgacatg
     3121 tgaaacgcgt tcgccaagat tgcgctcatc aaaataatgt tggaccatca tgatctgtgc
     3181 aacaatttct ccagcagtca gatcacggtt ctttttgagc agcccgcttg cacaaaacgt
     3241 acagccgata ttacatccta cttgtgtcgt cacacaaact gacaagccat attcttgtct
     3301 catcagtaca gtctcaatca tgtttttatc agggagttca aacaaatatt ttactgtacc
     3361 atccgaagct tcttggacga ccatctgttt tagaggattg atgataaaat gctccgataa
     3421 tttttcgatc aattgtttcg atagattcgt catttctgtg aaattactta cacgtttttg
     3481 atacaaccat tcccaaactt gggcagctcg gaattttttc tcgccgtttt ctataaacca
     3541 attgattaat tcttcattcg tcaagccata aatggactgt ttttccaaag tattttccct
     3601 ctttttctca aagtttcgct acatactata catgattcat ttccttaatg caatacttca
     3661 caaataaatc gaatagaact ttaatctcac aaaaactaaa aagacaagac atcataccgt
     3721 attagaaaag atggaaaaaa accatttttt cttttggtat catcatgcct tgtcttttta
     3781 ttggttattc gtttttcaac ccttcatcac acaagtttta catgtcatta tcgtccggca
     3841 acgtttctga tgtttcttcc tgctcaatga atactttccg aggcttgctt ccttctgacg
     3901 ggccaacaac tccatgagct tccagttcat ctactaaacg tgctgctcgg ttgtaaccga
     3961 tccgaaatct tctttggaga agggaaatac ttgccgtttg catttcaacg acaagtgctt
     4021 ttgcttcttc aaacagctca tcttgtggct gttctggagc acctgtcgtt tcaacttcat
     4081 ctgtcggcat cattttctct tcataatgag cttcttgctg atctgtaaca aattgaacga
     4141 cccgttctac ttcgtggtca gaaatgaatg ctccttgcac acggatcggt ttattctcac
     4201 ccattggcaa gaatagcata tctcctcgcc ctaagagctt ttctgcccca ttagaatcaa
     4261 tgatcgtccg agaatctgta ccgcttgata ccgcaaatgc cattctggaa ggtacatttg
     4321 ctttgatgat tcctgtgata acatccacac ttggacgttg agtagctagg atcatatgaa
     4381 tccccgctgc ccgagccatt tgcgctaaac gaataatcgc atcttcaact tcattgctgg
     4441 ctaccatcat caaatcagct aattcatcta cgatcactac gatgaatggt aaggttggat
     4501 gtttcgttcc gttttccaag tttttctgtt gaacaaattc attgtaacca gaaatatttc
     4561 ttactccggt tgccgcgaat ttttcatatc gttcttccat ttcttgtact actttttgta
     4621 aggcttgcgc tgcttttcta gggttcgtca cgaccggcgt caacagatga ggtatgccgt
     4681 tgtacatatt caattcaacc attttcggat cgatcatcat cagttttact tcatggggct
     4741 tagcacgcat taatatactt gtgataatcc cattaattgc tactgacttc ccactaccag
     4801 tcgaaccagc cacgagtaag tgaggcatct tcgaaagatc agccgtttgt acccgtccag
     4861 aaacatcacg tcctaaaggt acttctagta atttatctgg gtggtttggt tgtgcttcga
     4921 tgatttctct aaaggaaacc atactgatct ttccatttgg tacttcaata ccaatcagtg
     4981 atttacctgg aatcggtgct tccatccgga catctttcgc cgctaaagca agcgcgatat
     5041 catctgttaa gttcacaatt ttacttactt ttacccctac agccggctgg acttcgaatt
     5101 ttgtgacgga tggtcctaag ctggctttga cgaccttggc atcgacaccg aaactttgga
     5161 aagtttgttc taatacgccg atattttttt caattttttt atactcatca ctttgatcca
     5221 cagttggtat agaatcaagc aaatcaactg tcggtaattc atagtcttga ttttcctgtt
     5281 ctgcttcaat ctcaaattct aatgaggtac cgtcatcctc tggcaactca tctgaaaaac
     5341 cttctgctgt attggtctgt tttggtgtat ctggtactgg tttctctaga ttcgcaggat
     5401 ggatattttc ttgaaaactg tcgattggaa caaaacttag ctgctccggc tctgtatttt
     5461 ctggttcctg ccattcatca gcagtatttt ccctttgctg gctcaccgct tttttcttct
     5521 catattctgc agcttctttt tctgcagctt cttttgcagc taagcgcctt gcttctttcg
     5581 cttctgcgcg ctgtttcatt ctctcttcct ttgccgcttg ctttcgagct tgcttggcag
     5641 gatcaccttc caataattcc tgtactcgtt ctccaattga ttggatacca tttaacagct
     5701 gatagccttc cagattactg aacaggaatg ctcctcctgc cagtaatagc actgctgcta
     5761 aataacttcc aggctgtgca atcaagaagt aagtaaaatg ataaaggatt gctccaatca
     5821 tcccaccacc aacgttattg tctacttgac tttgttttat atcactggct aacaaatccc
     5881 atgtcgtccc catgatattt ggttccccag tatggagttt gccaaataaa tgggcatgta
     5941 acaataataa gataccgaaa taaaagagta tgccgccaaa ccagcgtcgg tttttaccaa
     6001 ttgaaaactc tgtattctta atgacaatcc aaaatcctaa tacggctaat aaaagacaga
     6061 ggatttggta agtatttcct gcaatgatcc gaaagccgtt tgcaatcaat attcctaaaa
     6121 aacctaattt taaaaagccg aagacagcaa ataaaatcaa ggcaaaccca acaccaatcg
     6181 tcagcatttg ttcctgctgc tgtttttgct ttttcgtttt tttcgtcttt ttcctttttt
     6241 tagtaggccg tttcttttgt gccatttatt atccctcatt tcatcaagtt tcattataca
     6301 aaaaaaagta agggaactaa agttttgttt gaaagaactg acgctttctt tcatttttca
     6361 gcttatttta cagtttttct taatttctaa atgattttat caagaaaagg cacggccata
     6421 tccagccgta ccttcttgtt ttaagtatgt gaactaaagg ttctctccat tttcttcaat
     6481 cagtttttgg taccagtaat aactgtcttt cttatatcgt tccaaagatc cttcttgtgt
     6541 ttcgtcttga tcaacatata caaatccata tcgcttttga taaccgttca gccagctaag
     6601 caaatctgta taagaccagg tgcaatagcc taggacttct gctccatccg aaattgcttc
     6661 tttgatggct ttgacgtggc tttgtaagta attgattcga tagtcgtcat ggatttgttt
     6721 atcttctgtc aacttgtcat actctcctaa gccattttcc gtaatcagta ccggcatgcg
     6781 gtaacggctt gtgatacgac gcagtccgat ccttaatcct tctgggtcaa tctcccagtc
     6841 ccaatttgtt cgttcgacaa atggattctc aactttttta tacattccag gaactccagt
     6901 ttcttctgat gaaccttttt ttcctgtcgt attcattttt ccagcaccaa ctccgtttaa
     6961 tggattgaat gcattcgtcg ccgtttgata gtaattgatc ccaagaaaat ctggtttagc
     7021 agattctagc aattctgcat cgccagcttc aatcgttggt gcccatcctt tttcttctaa
     7081 gaacctcatg gcagcaatcg gatattttcc ccacatatag acatccatcc aataattggc
     7141 catcaggtct tctgcatttt cagcagccag aacattttca ggattactgt caattggata
     7201 atttggcgta tacgcaaagc ttggtccgat ttttccaggc atttctaact cgtgaaattt
     7261 attgatgaca gatgcatttg ctaaattagc aatatggtta acttcataca ttcgtttcgg
     7321 gtctgtcaca cctggcggat gtgctgctaa caaataacct aatgaagtga agatattttg
     7381 ttcattcaaa ctaatccagt aatggacttt cccgcggaat gcctcaaata atgtttctgc
     7441 ataatgagtg aaatcttcaa tgatctgacg tgattcccaa ccattatatt catcttgcaa
     7501 tgcttgtgga agatcccaat gatataaagt caacataggt tcaatatcgt tttcaatcaa
     7561 ttcgtcgatc aatctctcat aaaaagcgag cccagcttga ttgacttctc ctcgtccatt
     7621 aggaaaaatc cgtgtccaag ctacagagaa gcggtaagct ttcagacctt gttctttcat
     7681 taaagcaata tcttctttat aacgatgata gtggtctact gccaaatcac cgttcgttcc
     7741 tttaaatgtc tttccaggta tccgtacaaa tcgatcccag actgattctc ctttcccatc
     7801 ttcttgccaa gctccttcta cttgatacgc tgcagaagca gagccccaca gaaaatcttt
     7861 tggaaatgag gtaagttctt tgtgttccat tcatctcttc ctttcctata accttcaatc
     7921 gttcctcttt tagaataaca cgaaacccct tccacaacaa aataaaatta tgtaaaggag
     7981 gttgtgaaaa agtcgcttag tttcgagta
//
LOCUS       DASJON010000132         8000 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJON010000132 DASJON010000000
VERSION     DASJON010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.134"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..335
                     /locus_tag="U3989_002100"
     CDS             <1..335
                     /locus_tag="U3989_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN1715537.1"
                     /translation="FRRLRKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGVL
                     TYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIKS
                     IQESEGKI"
     gene            424..729
                     /locus_tag="U3989_002101"
     CDS             424..729
                     /locus_tag="U3989_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN1715538.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            908..2923
                     /locus_tag="U3989_002102"
     CDS             908..2923
                     /locus_tag="U3989_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1715539.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            2925..3209
                     /locus_tag="U3989_002103"
     CDS             2925..3209
                     /locus_tag="U3989_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715540.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            3235..4608
                     /locus_tag="U3989_002104"
     CDS             3235..4608
                     /locus_tag="U3989_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1715541.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(4691..6706)
                     /locus_tag="U3989_002105"
     CDS             complement(4691..6706)
                     /locus_tag="U3989_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN1715542.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6728..7942)
                     /locus_tag="U3989_002106"
     CDS             complement(6728..7942)
                     /locus_tag="U3989_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1715543.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
BASE COUNT         2551 a         1496 c         1542 g         2411 t
ORIGIN      
        1 agtttcgtcg tttacggaaa aaagggatgt tcgtgaaaca aacaaaagat cgacaagaag
       61 aacatctgaa aatgttttac atgcctgatc atgaagactt tggtatcgat ttgcccgaac
      121 attatggtgt attgacctat atagatgaac aagggagtta tcatgaagaa aaagtaattt
      181 ccgaggttgg tgattacagc cgtgtctacg aagggattta cgagacatta gttaatggcg
      241 cagaaaaagt catcaaagat gaagaaacaa tcttacaaat gaaaattttg gaagaaggaa
      301 tcaaaagcat tcaagaatca gaaggaaaaa tatgacatat ttgtaagaat cgggtttgtt
      361 ttttgacgca taaactgtct tattgtacga tgaaaaagta tagaactact aggaggaacc
      421 aacatgacaa attatgacgg tgaaaaacac aaacgattaa gaggttcaat ggcccctaaa
      481 acagaagaag aagtggcttt agcaggaact aattctggtg aaacagaaga caaagaagaa
      541 tcattagctg gcacaaactc tggtgaaaca gaaaataaag aaacagcttt atctggttca
      601 aattctgtag aagaggaaac aaagcctaat gagcccaatc actttgaaaa agatatcgaa
      661 aaagcggttg aagatgtcaa agaaaaattt gaagaactga cctcaaaaaa taaacatgat
      721 catcattgat cggacgaaat caattcataa aagcaggaga catttggtca cctgcttttt
      781 ttctgtatcc attcgataga aatcgttcgg tacagtcgta aaaaaaatcc aactgtaagt
      841 tgtaccaaac acccctcgat ttaaaagcgc taccatacta gaatataagt aggaggtgaa
      901 cagcgctgtg aactacaaaa ttagttatct cctgaaaaca ccaagagaaa tcagtctaga
      961 aaaagcagca gagatcatcg aagtaaatac aagcgaggcg atcaaacaat taactccttt
     1021 tgccgaatat gtggatgata cccgtatccg tataaagaaa ttccgagcaa tcgactgggc
     1081 agaaaaattt ttggatatta ctcgatttga agctgtgtat accgaacaag agcgaagaga
     1141 tcttctttat ttgatggttt attcgactgc aacgtctgtt tctgtttttc attttcaaga
     1201 ttttcttcag gtttctaaag ggacagtcct gtcagatatc aaaaagataa gaaatgaatt
     1261 agaagtggaa gattgtcagc tgtcgtatac taggaaaact ggttttaggc tgctagggtc
     1321 agaagcaata ttaagaagag aagcaaaaaa tcgcactgct cgtttgatgc agtcacttgc
     1381 tggaagattt gggctcacgt atttaaccgc aaccctttcc attgattatt acgccaaagt
     1441 tagagatttg ctagataaaa cgatttcact atcagatctt cgattcgcac caaatcgaat
     1501 ggatgaatta gcttattatt tgagtttgac aaccattcga attggaaaaa catccatgga
     1561 actggtaaaa gatgctgaac tgttggcttc tcttgcagtc actgacatta gtcgacaagt
     1621 attgactggc cttggctatt ccgaacatca aaattcggag attatatttt tctctctttg
     1681 cttagcatcg atcgttgaag gagatgtcca ggagcctgca cttgattttt tgctggaatg
     1741 ttcttcagaa gtcatccatc gcatggaatt cctgatggcc gtagaatttg cttcgtttcg
     1801 tgaattattg atgaatgtct tttatcactt ggtccctgcc tattttcgta tttcctattc
     1861 tttttattta cctaatgtga tgatcgatca aattaagcat caatatgctt ccatctacga
     1921 aatgacaaga aaagcattac ttccgttaga aagacgaata gggaagagca tccctgaaga
     1981 agagattggt tttttcacta tactatttgg cggagagatc agaaaagcgg acgcagaaga
     2041 aagaaataga aaaataagag ccgtgatcgt ctgtccaagt ggcattagct cttcattgat
     2101 cttaaaaagt gaacttcagc aattattccc gatgatcttt tttacagaga ctaattcagc
     2161 tgaccgctta cacgaggttg acgaaacttc ttacgatatt attttttcta ctgtacctct
     2221 cacacttact gataagaaaa agtctctcta tgttctgcat ccaatcatga ctgacttgga
     2281 aaaaaatcag ctgatcaatc aagtacaaag agattggctg ataccaggat tttcaatgcc
     2341 ttctgcccaa gaattgttgt cggcgctact gccttatatc gaattaaaag aaggcgtaga
     2401 cgaagaaaag ctctatcaag tattaaatcg taaaatgaac aaacttttag aaaagaagga
     2461 ggatcaacgt cctatgctat ctgaattatt aacagaagaa atgatccaat tatcggatca
     2521 accaaaaaac tggcaagaag caatcacatt tgccgcacag ccgctattat cagaaaataa
     2581 aatagaagaa agttatatca aagctatgat cgagcgcgtg gagcagtatg gtgcgtttat
     2641 ccatattggc gatcacatcg cattacctca tgctcgtcca gaagatggcg taaacgaagt
     2701 agggatgtct ttgctgaaac ttcaacaacc tgtggatctg cttgataatc cagcccatcc
     2761 cattagtttg ttcatctgtt tagctgctat cgataatgag gcacatcttc gggctttggc
     2821 aaatttaaca aagctacttt ctaataaaca aaatcttcaa gatttattac acgcatcaac
     2881 aaaagaagaa atattagcga tcataagaaa aggagacgaa tgatatgaaa atttttgcag
     2941 tatgtcaaag tggattagga acaagtttta tggtagagat gaacatcaaa caagcacttg
     3001 cagatcttgg agcagatgtg gacgagtttg aagtaagtca tacggatgtt ggaagtgtat
     3061 ctagcgatat ggctgattat ttcttcattg agcattcttt gaaagatacg ctaacttcta
     3121 tacctgatga aaagctcgta cctttgcaat cgatcattga cagcgaagaa gtaaaagaaa
     3181 aagtaacgaa gatcttgtca aaagaataaa aatgattcaa gggaggaact atcaatggat
     3241 aaattgttga atttgtttgt gtcaatcgtc agtcagccct ccatcctcgt ttctttgatt
     3301 gcactgcttg gtctcgttct tcagaagaag aaattttcag aagtgatcca aggaactatc
     3361 aaaacatttg tcggattcct agtactgatc ggcggtgctg gtttaatcag taattcacta
     3421 accccttttg cttcgatgtt ccaaacagct ctgcatactc aaggagtagt tcctagcaat
     3481 gaagcagtag tggcgattgc tttgcaacaa tacggtacac ctactgcttt gatcatgcta
     3541 gtagggatga tcgtgaatat tttacttgct agattcacaa gatataaatt catttttctt
     3601 acaggtcaag caatgcttta tgtttcttgt ctaacagcag tcattttagt aagcgcgggg
     3661 tttactgcaa atttgtggat gatcttatta ggcggggtct tcgaaggaac actattaact
     3721 attacgcctg ctctttgtca accgtttatg aagaacatca ctggaaacaa tcaagttgcg
     3781 ttagggcata ccggaaatat cggttatgct tctagtggat ggatcggaaa gttttttgga
     3841 aacaaagacc gttctacaga ggacataaag attcctaaaa gtttcagctt tttgcgagat
     3901 tctactgtat cgatcatgct cttgatgtcg atcgtatatg tcgcacttgc tttgttagct
     3961 ggtcctggat ttgttgaaag agaactaagc aatggagaca acgctgtaat ctttgcactg
     4021 atccaagctg gaacattcac agctgggttt gttatcgtcc ttcaaggggt tcgaatggtg
     4081 ttggcagaaa tcgttccagc attccaaggg attgctaaaa aactcgtacc cgattctaaa
     4141 ccagcattag atgttcctat cgtttttcca tatgcaccta atgctgtatt aattggcttt
     4201 tttgttagct tctttgtagg aacactatcc atgtttgcta tgataggctt gcaaactaca
     4261 gttatcattc ctggggtagt aggacatttc ttctgcggag ctgcttctgg tgtcttcggc
     4321 aactcgactg gcggtagaag aggggcggta ttaggttctg ctttcaatag cttattgatt
     4381 agttggctgc cattatttat ccttccagta ttaggagatc ttaaaatcgc ttcttctaca
     4441 tttgccgata ctgattatct agtaccagga cttgtacttg ggaaactagg agaatacggt
     4501 caaatagcct tgacagttgg aatcatcgct ttctttttac ttgtcgtatt atctagcttt
     4561 ctttttgccg gaaaaagaac aaacattgtt tctaaagaac agaattaagc aaacttcaaa
     4621 ataaaaattg cttggacaat atcagaagaa gatttaaagt cttctgatat gtcgcaagca
     4681 gttttttgtt ttatgcattt atttttgaat agcttgttaa gacaagtggc tgttttgcta
     4741 gaagaggata acttttacca ttcattttca ctgttccgcc tgttctatca gaagtaatca
     4801 cgatcttatc ttttgttttt tcaagcgaaa tctttccatc tttgataggt agcgtacaat
     4861 tcagtgtttc aaatgaatct agattaggtt gtatcataaa ttcttcataa cctggctttg
     4921 ttggatacaa accgataaaa tagcgtccta aaagatagat tgggcttgct ccccatgcat
     4981 ggcataagct ttttccataa ggatctccat acatactgta cttttcattt cctgtttgag
     5041 tagggtcgta ttcttcccag aaagttaccg catcatgaga taacatccct ccccaataat
     5101 caagaatcgt ttggtagacg atattcgtct gtccgagttt gcataaagca tcttgttcat
     5161 agaatttgaa atatggagta gtaatctgtg tgatagtgtc attcaataag acattcttta
     5221 agatcgattg ctgttggtct ttgttagcaa aatcgaataa aacagcgaaa atattcgcat
     5281 gacgcgtcac atttcttctc ccagattcat agcaatcgat aaaagcgcca agttcttcat
     5341 cccaaaagta gtcaaatagg ttttgtttca atagttgata tttttcttga tagagatgag
     5401 tatgataatt ttcgttctct ccatatgttt cagctaagat atctccgcaa gtgatcatgg
     5461 aatgataggc tttcagtaat aagacttgtt ctgcagcaag cgttccttct ttatccattt
     5521 cagaccaatc aataaatatc caatcttttg ctctcccata aataaaccct aattcattcg
     5581 tttgctccat caagtatctc attaatgatt gcatcttagg gtagacgatg cgtaaaaatt
     5641 catagtcttt cgtcatcata tagtggtttt cgataccaat caaccacaag atcgaatagt
     5701 caacgatagt attcatatgc tgcttgattt cattttgtcc tcttaatgct aatatcgttc
     5761 gtttattgat ttcctcgtca aaaaaaagat attgattgat catgaatgat tgatacgcat
     5821 ctccagacca aatccagcga tctcgtttga taccgtcgat aaaaaacaaa tcactacata
     5881 acttgaaggc ttcttctgat actgaccaga tctgattcag taaagaagaa tcgcttgaaa
     5941 aagcagcttg tttcgaaaga gataaaaatt catgaaaaac ttgaagttga accattccag
     6001 tagaaacctc tggtataaac aaatagcgaa aagcgcgttt acgagctttc atttttcttg
     6061 tcattcctat ttgcttatag tagcaataat cgatatccat agcttctaca tcggactctc
     6121 cataacatag agtgatagat tcgatatggt catccagaga tattagttct acacagccat
     6181 tcactgctcg cccaaaatcg aataagattc ctccgccaat ctccgtttca gtgactggat
     6241 ggacgcattc tttttgataa tagatttgat ttggatcttt tgcgatatcg acataaagcg
     6301 gactccagcc agctggatat tcttcaataa aattacttgc tgtccagcca atatctgatt
     6361 ttacttcgtc tccctcgata aacatcgctg gtagccctga tgtatgtcct acaaagatac
     6421 gaatttttgc tttaccagct ggacaagtca gccattttcc acatggatgt ttttgtccat
     6481 taatttctac atacccaact ccttggatcg tcactttaaa catggaaggt tgatcaagaa
     6541 agtaatatct cttaaacttg acgttccgat ggcaatcatc catgtaccag tacgctggcc
     6601 agtcaaaacc tcgttcttcc cgttggaaat tttgtagcag tccatggcgg atctcaaagt
     6661 ctccaggata ccaaagccat ctatctttat gtttttcatt tgtcatttta cacgacctcc
     6721 atcaaattta ttttgttgtc aattgttctt ttatttcttc atctactctt atcactgtct
     6781 ttctattcgt cctgtgtact actagaaggc taataagcgc gctaatcaac agcaagatac
     6841 tgccattgac gaaaacagca agtgttttac tcccaagaga agcaaaagag agaaagagca
     6901 aaccatatgg tgcaatgaaa gtacctaaat tagcgccaac caataagatt gaagtattga
     6961 aactctccgc gtacttgggc gcttggatac ttacttggtt gaataagtaa ggaatgaata
     7021 acgaaaaaga aattcctgtt aggatcgcac caaacccaat cacatacagg tttatcacag
     7081 tggcaatcaa tacaaagccg attcccatca gtagtaaaga aagtgtaagc atatagcgat
     7141 gtgcttttgt ctgaagccag ccaaaacaaa ctccggcagc catacttgcc aattgcatga
     7201 ttgttaagat attcgctgca ttagtttcgt ttcctagaga ataagtcgtc aggactgtgg
     7261 ctaatttaat cgatacagca ttatatacca cgatgaccag caagagaaaa ataccataac
     7321 taatcatttt aggattcgtt ttgattactt gtcttgttgg ggctgtgcca tgcgattgaa
     7381 gttcagttgt ttcaggtgta ggaacaaaca aaacaaacaa gacaagaata ggtaagctga
     7441 tggcgtagac taaaaaggta tattgccaac cgaactgtac tagctgtcct gcaacaaatg
     7501 tcattaaagc tgcaccaaat ccttgaaatg cactttgaaa accaatcata gaggctcgtt
     7561 catctccgtg ataaaaggca gcaatcatgc tgactgccaa agaattgatc aatccaaagc
     7621 caatacctag tccagctctt gaagctaaga caatactgta actttgtgag aagaggggga
     7681 ggataccaca aatcgatgca atcactaatc cgatgatcac agtttgtttt tgtccgattt
     7741 tcctagcaat aaaactacta atcaaaacaa aaatagtgac cataaaagag ggagcagtcg
     7801 ttaataattc aacagcagat aaagaacgcg tggcataagt agctgacata ctgggaattg
     7861 cagacgtgac tgcaccagca gaagtcagaa taagagagac tgacagcaga gaaaatttca
     7921 tcatcttttt attcataagc atgttccttt catttttgta agcgcttatc aaaaatatag
     7981 tatgaagtgg ttattctctc
//
LOCUS       DASJON010000133         7983 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJON010000133 DASJON010000000
VERSION     DASJON010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.135"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1196)
                     /locus_tag="U3989_002107"
     CDS             complement(<1..1196)
                     /locus_tag="U3989_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1715544.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYE"
     gene            1522..2148
                     /locus_tag="U3989_002108"
     CDS             1522..2148
                     /locus_tag="U3989_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1715545.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            2171..2875
                     /locus_tag="U3989_002109"
     CDS             2171..2875
                     /locus_tag="U3989_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1715546.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            3067..3900
                     /locus_tag="U3989_002110"
     CDS             3067..3900
                     /locus_tag="U3989_002110"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN1715547.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            4097..6025
                     /locus_tag="U3989_002111"
     CDS             4097..6025
                     /locus_tag="U3989_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1715548.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            6163..6618
                     /locus_tag="U3989_002112"
     CDS             6163..6618
                     /locus_tag="U3989_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN1715549.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            7088..7357
                     /locus_tag="U3989_002113"
     CDS             7088..7357
                     /locus_tag="U3989_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715550.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            7757..>7983
                     /locus_tag="U3989_002114"
     CDS             7757..>7983
                     /locus_tag="U3989_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN1715551.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKS"
BASE COUNT         2428 a         1454 c         1519 g         2582 t
ORIGIN      
        1 ttttcataag atagttctgc cgaatacatc tctcgggaac gattcaccaa attatgtgct
       61 tcgtcaatca aaaacagata gtcctcgttt ttttcatctt caaaaaaacg tcttaaataa
      121 acagttggat caaataaata attgtaatcc ccaatgatta cgtcacacca gatactgaca
      181 tctaatgata gttcgaaagg acaaagtgta tgctttcttg cataagtttc gatgacttca
      241 cgagtgatct gattctcatg attcaaaaga tcccataaac cttcattaat ccgattgtaa
      301 taaccattgg catatgggca ctgatctgga ttacaggtcg tctcatctaa aaagcagatc
      361 ttgtctttcg ccgtcaaagt gacacttttt acttcagctc ctgtatcttt taatgctgtc
      421 atagcatctt cagctacttg tctagttatt gttttagccg ttagataaaa gatcttttct
      481 ccctcttctt cccctacagc tttcaaagcg gggaataacg tagagattgt ttttcctgtt
      541 ccggtaggtg cttctacaaa caatttctgt ttcgtacgga tcgttttata agcagctacc
      601 gctagttcac gctgaccttt cctatactca tcatggggga atttcaaagc catcaaagag
      661 gcattgcgta cagtgcgcca gttttcttga aaaacaagcc attctttata atcttcagtc
      721 agctttttaa aaaaagtttc taactgctct cttgtctgat gttcaacctt tctcgtgatc
      781 acttcttctg ccgtctggaa gtaagtcaat tgcagattta tttcttgtaa atcatgttct
      841 tgactataga tataggcata cacccttgct tgatgaaaga aaagttccac ttgttctggt
      901 tctaaatctt caaatcgtgg ttcggaggtt ttgatttcat ctatataata aataccgtct
      961 cttttgaaaa taccgtctgc acggccttca acgatcaggt aatattcatc taattgaact
     1021 gttgttttta aaaagacttc tttttcatag ttttctcccg catctttttg gagcttgcgg
     1081 tggattctcg ctccttctaa tgcagtatgg ttactttttt tacgattgtc gatacttcca
     1141 cgtcgcaaaa taaattctac taagtgccgc acagatattt ttgactgttt gatcatttcc
     1201 accacctctt tctctctaag attacctgaa aaaacctagt ttagcaaaaa gagctctctt
     1261 tccaaacata aaaaaacacc ctgctgatta ggcagggata taggatatag aatttggatt
     1321 tttggatgaa tctaatgtaa caaggacata ttaaaatgct atgaaaactt actttccttc
     1381 tctttagaat atccaaatac ttttcatata gtctgataat tcagataagt aaagagaaat
     1441 aagtacctgt ttttgtttcc tagcaatgaa tgcgttatac taatggatga aagaagtttt
     1501 ggctatcaag gaggaatgca catgcatatc gaaagattaa aaacaggaat gattgaagaa
     1561 aactgttatt tggtctacaa cgaagaagca cttttgatca ttgaccctgg agaagatgcg
     1621 gaacgaatca aacaagaaat caaaaaaaca aaccaacagc cggtagcgat tttgcttacc
     1681 catactcact acgatcatat cggtgcagtg gaacctttac gcgcgtatta tcaaatccca
     1741 gtttatgtca gcccactaga gcaaaaatgg ctaggtgatc cgattttgaa tctttctgga
     1801 ctcggtagac atgatgatat tgctgatgtt attgtctcgc ctgcagaata tgaatttgaa
     1861 atgaaaactt atactttagg aaacatgtct tttcgcgtcg tccctacccc agggcattct
     1921 ataggaagcg tcagctttat ttttgatgat tttgtcgttt ctggcgacgc cctgtttaga
     1981 ggaagtatcg gtagaaccga tctgtataca gggaatttag aacaattact tcatagtatt
     2041 cggacccagc tgtttgtatt acctgatgaa tttgcagtct atccaggaca tggcgatgca
     2101 acgacgattg aacaggaaaa aaggacaaat ccttttttca atcgttaaaa cgacttagga
     2161 ggagaataaa atgacagaaa ctacacttta tatcgtacga cacggaaaaa cgatgttcaa
     2221 tacaattgaa cgagtacaag gctggtgtga tacacccctc acaaagcaag gacaagaagg
     2281 cattcattat ttaggtcgcg ggttaaaaaa tgtcgacttt tcatttgctt attctagtga
     2341 cagtggtcgt gcaatcgaaa ctgcccgtat tattctagga gaacattcta agggaaaaga
     2401 aattccttat tttattgaaa aaaggattag agaatggtgt ttcgggtctc ttgaaggtgg
     2461 atatgacatg gaaatgtggg gtgtgatccc tcgtgtattg aattttaaaa catatgatga
     2521 aatgtttacc acagaagttt cttttgaaca aattgctaat gctgtataca acttagatac
     2581 cgctgggtgg gcggaaactt atgaaacatt gcgagaccgt gtatggacag gatttgaaga
     2641 tatcgcccat cactgcgaaa aaaatggtgg tggaaatgtt ttagtcgtat ctcatggttt
     2701 gacgatcgcc tttttgctta atctgatcga tcctaaacag cctgttcgtg ctgggctttt
     2761 aaatggcagc gtaacaaagg tcacttatgc aaacggtcga ttttctatcc acggcatcaa
     2821 tgatacgtct tatatcgata acgggaaaac acatagtcgc gactcgtttc tataatgggg
     2881 atattttaat ttttaccaga gaatcataaa aggcaaagtc tgaacgaggg ctttgtcttc
     2941 ttttttctct tcgtgtactt atctgcttta tttctcaagt ttttcttaat attaaaaaac
     3001 agattgggtt tctcaaaaaa taacggtata ctataagatg tatcttaatt gggaaggaag
     3061 gaatttatgt tttttgcaaa tattatcaaa gcagtgattc tcggtattat tgaaggaata
     3121 acggaatggc tgcccatcag tagtactggt catttgatcc tagcagatga attcatcaaa
     3181 ctggatgcca gttcacaatt tatgtcgatg ttcaatgtcg ttatccaatt aggggcgatc
     3241 ttggctgtcg tcgttcttta ttttcacaag ttaaacccat tttctccaag taaatcaacg
     3301 gtagaaaaaa aagatacgtg gacactttgg tttaaagtga tcgttgcttg cataccagcc
     3361 atcatcattg gtttgccttt ggatgattgg ttagaagcaa atttccacaa gttcttacca
     3421 gttgccattg ttttgatcgt ttacggtatt gcctttatta ttgtcgaaaa acgcaacaaa
     3481 aacaaaacac ctcgttggac ttctctgaat gactttacgt accaagcagc tcttcttgtt
     3541 gggttattcc aagtcttggc acttgttccc ggaacatccc gttctggcgc aacgatccta
     3601 ggatctatct tgattggtgc ttcgcgtttt gtcgctacag agttttcatt tttcttaggg
     3661 attcctgtca tgtttggcgc aagcggcttg aagatcgtca aatatttagc tgaagggaat
     3721 tcattcaaaa tggaagaaac tgttgttctt cttgtaggaa cattcgtatc ttttgtggtt
     3781 tccgtcttag ctatcaaatt cttactaagc tatttgaaga aaaacgactt taccgctttt
     3841 ggctggtatc gtattatcct tggtatcatt ttgattggtt attggtttat tgcaatgtag
     3901 aaaaaaccag aacacttctc ttatttgaga aatgttctgg ttttttattt tctttaataa
     3961 taatcattat aaataataaa aacaacatca agcttaatca ccaaatgata accattatcg
     4021 ttctcattta gctgttatta ttatcaatta catttttatt atgctatcct tgttttgaca
     4081 aggaggtcta ataacgatga ctaatcagaa aaaagctttt gcagcaacaa tcggctgcct
     4141 tttatttatg atcttcggtt ttatcttcga ccgattctct cttaccatca ccccactttt
     4201 ttatatcagt gccattatca tcggtggata taaacaaaca tcggaaggct tgaaagaatt
     4261 gtggaatgat cggacactga atgtggattt attgatggct ttagctgcta ttggtgcctg
     4321 tttgatcggt aattactttg aaggagcaat gttgacattc atcttttgtt taagcggtgc
     4381 attagaggaa tacacaacta ataaaagtca aaaagaaatt actgctttga tgaatcttca
     4441 accacaaaaa gcccaattat taaaagaaaa tggacaaatg caagaagtag atgtttctca
     4501 actaaaggtg gataacttgg tatttgtcgc taaaggagca gctgttccga tagatggctt
     4561 tttagaaagc acacaggcaa cgatcgatga gtctgcatta agcggtgaat ctgtgcctgt
     4621 agaaaaatat ttaggcgatg agatgtatgc aggaacgctg aatcaaggaa atcccatcac
     4681 gattcgagta acaaaaacga gttccgaaac tgtctttgct aagattatcc aattagtaga
     4741 agaagcgcag aatacgccaa ctcaaacagc ttcatttatc gaacgaatcg aaaataatta
     4801 tgtaaaattg attttgcttg ctgttcctct aatgatcctt ctcccccact ttttcctagg
     4861 ttggagttgg gatgaaagct tttatcgagg aatggtatta ttagttgtgg cttctccttg
     4921 tgcgcttgtc gcatctgcta caccagcttc tctagctgct ctttcaaatt ctgcaaaaaa
     4981 tgggatttta atcaaaggcg gtatccattt agaaaagctt tctgaattaa aagcgattgc
     5041 ctttgataaa acaggaacgc tcactaaagg aaaaccagtt gtaactgaca gctttttctt
     5101 tgatgaaaag gatttggcac aacaacttct tgttgctatg gaacaaaaaa cgacgcatcc
     5161 acttgctcat gcgattttgc aatatttcga ttgcacaata cctgaagaaa tccaatactt
     5221 accaatagaa gaaattactg gttttggatt acaaactacc tatttaggtg cgcaatggaa
     5281 agttggcaag cacgcatatg atcctgagac gatgataatc agcaaagaga ttgcggaaat
     5341 gatcgaacgt ttggaaaatc aaggcaaaac cgtgatttat ctttctaaag atcagcagtt
     5401 aattgctgtt ttaggtctgc tagatattcc aaaagcaaat acccaacaag taatttccta
     5461 ctttaaatcc caaaacatcc atacaagtat gataaccggc gaccattctg gcacagcaaa
     5521 ggccattgct gaacaagtag ggattgaaca ttactatgca agctgtactc cggaagaaaa
     5581 aacacaacta gtcaaaaaag agaatgaact ttaccaagta aatgcgatgg ttggtgatgg
     5641 agtcaatgat gcgcctgctt tagcagccgc ttctttaggt gtcgctatgg ggcaagggac
     5701 agatgccgct atggatatcg cagacatcgt cttaatgaaa agcgacttaa ataaatttgt
     5761 ctacagtcat cggctatctt taaaaatgcg gcggatcatc aaacaaaata ttattttttc
     5821 gattcttgtt attctcttgt tgattgcttc taacttcctg caatttttaa atctacctct
     5881 tggagttgta ggacacgaag gaagcacaat attggttatt ctgaatggct tgcgtttact
     5941 aagaccttta cctgatcttt ctgattcttc aaaaaaatgt aagtcttgtc cactatacaa
     6001 agcgcggctg tcttctgtca aataagcaaa gtgttttttt atgattttgt ttattttatg
     6061 aaaaaaacag cttatgaata ggtcttgaag gatgaattcc tacttttgta actttctaaa
     6121 aacatgttat gattgtttcg aagaaagtga ggaatatatt tcatggaaag ttggcttttt
     6181 ttaggaatta ttttacttgt agcaatttta gctaaaaatc aaagtctgat gatagcggca
     6241 gcttttgttt tgattatgaa gctcatacca gcctcccgtt cctttttccc ttggcttcaa
     6301 ggcaaaggaa tcaatattgg ggtgacgatc atcacagcgg ctattctaat ccctatcgca
     6361 acaggagaaa tcggtctacg tgatctatta gaagccttta aatcccctat tggctgggta
     6421 gctgtttttt gcggtgggct agtggctgtc ttatcttcca agggtgtagg attgattgct
     6481 tctgatccac aaatcactgt tgcacttgtt ttcggcacga ttttaggtgt tgtcttctta
     6541 agaggaatcg ccgccggacc ggtcattgct tctggtatca cttactgtat cttacaagta
     6601 tttgctttag gaaaatagaa caaattttgt ctattttagg agattcttaa cacccccaat
     6661 ttactgatcc gtgatatact gacaacgagg tgatacaatg ttcgaaaaat taaagtattt
     6721 cataggaaca atcggctggt ttcttctagc ggtacttttg attctgggct tagcttatct
     6781 attatttcct gatttgatac aaaatgtgtt tgatactgct ttttgattga cctattaaaa
     6841 cgatataggg gatgctcctg cgatgcaggg gcttttttta atcaatgttt tgtaataatt
     6901 taggccgtgc caatgagact tcctatagaa ttcgatgctc ttttttcttt ttggctcttc
     6961 tgctcttttt tgttatacta gattacagtg gacaaaagaa agaatgatcg catccacttc
     7021 aacgaatatc aaaatggaag aattagtgcc tgttttcagg ttactttgaa aagaaaggaa
     7081 gacgcttgtg gaaacttttg aaggtctttt aattgatacg tacgttttac aagaacagcc
     7141 aatgatgctg actgggatca acttgcagct tgaggaccgt gtgatcaatg gcctgcttaa
     7201 agatcgctat ttggcaaaat atatcttacg tttgccccta aataaatatg tgctggaagt
     7261 aagtggcaaa tggaaccgtc ggaaccagtt ggtcataaaa aaaatgttga taaaaaatat
     7321 cgatgaatac attaaaataa tcggtacacc agaatagcct ttactgatca aaggatactg
     7381 gttataaaat aactgcagtt tcttaataga aaaacaaaga acaacaggga atcgtatccc
     7441 ttttcaaaaa aaactttgca aacggttctc tttgttatat aataacttag taccaaacat
     7501 tcccattcaa actttgttta aatagataaa taaaaatcta gtttggtttg acgctattgc
     7561 ctcccacaat agcgtctttt tgattttttt cagcttgtac tatccgtttt tactgaaaaa
     7621 aaatcctaat aaaagactgt taagggaaaa aacttgtcat gttcatattg caaagcacgc
     7681 tgcctttagg tataataatg aatgttgaat tatgcggtaa cacgcgtaag aacttaagtt
     7741 atcggaggtg aaactaatgc caaacattga atctgcaatc aaacgtgttc gtacaagcga
     7801 aaacgctaat gtgaaaaatt catctcaaac aagcgctatg cgtactgcaa tcaaaaaatt
     7861 tgaagatgct gtagcttcag gtgctgacaa cgtagatgcg ttatataaag aagctgttaa
     7921 agctattgat atggctgaat caaaaggtct aatccacaaa aacaaagcta accgcgacaa
     7981 atc
//
LOCUS       DASJON010000134         7761 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJON010000134 DASJON010000000
VERSION     DASJON010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.136"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            147..1094
                     /locus_tag="U3989_002115"
     CDS             147..1094
                     /locus_tag="U3989_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1715552.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="U3989_002116"
     CDS             1106..2026
                     /locus_tag="U3989_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1715553.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="U3989_002117"
     CDS             2067..3524
                     /locus_tag="U3989_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1715554.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="U3989_002118"
     CDS             3869..4483
                     /locus_tag="U3989_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1715555.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="U3989_002119"
     CDS             4476..6200
                     /locus_tag="U3989_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1715556.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="U3989_002120"
     CDS             6197..7648
                     /locus_tag="U3989_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1715557.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2619 a         1291 c         1542 g         2309 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attagaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DASJON010000135         7743 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJON010000135 DASJON010000000
VERSION     DASJON010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.137"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(712..2058)
                     /gene="gor"
                     /locus_tag="U3989_002121"
     CDS             complement(712..2058)
                     /gene="gor"
                     /locus_tag="U3989_002121"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN1715558.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2192..3445)
                     /locus_tag="U3989_002122"
     CDS             complement(2192..3445)
                     /locus_tag="U3989_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1715559.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3451..4374)
                     /locus_tag="U3989_002123"
     CDS             complement(3451..4374)
                     /locus_tag="U3989_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1715560.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4595..7087)
                     /locus_tag="U3989_002124"
     CDS             complement(4595..7087)
                     /locus_tag="U3989_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1715561.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7094..7561)
                     /locus_tag="U3989_002125"
     CDS             complement(7094..7561)
                     /locus_tag="U3989_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN1715562.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2138 a         1676 c         1253 g         2676 t
ORIGIN      
        1 agatgcaagc ttttgaacac cgtttatcag gaggttgtga aaaagtcgct tagtttcgag
       61 tattaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac
      121 cgagaaactg gcttttgaac accgtttatc agaggttgta aaatcagtgt tttgtcctga
      181 gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt gagctaatac
      241 cgaaggacag ctgattgaac actatttgtt cggaaaaagt gactaactat gacacaattt
      301 ttttcacagt cccttttttc acttgatttg attcgtacca gcgaacttga cgagaacttt
      361 cttggtttgg taactatatg gtattctttt aacatcctcg tttgcttctt ctaatttatt
      421 tatacaaagt cacacttttt taaacatctg ttcttcgata catttctatt tcttttaaac
      481 atcgatacca atattttgaa cttaagagtg cataccaaat ggattcattt actcttcgtc
      541 agttacgaag ataatcaaaa tctccaactt ccctacttat ttcgttttga gaattatcga
      601 agcaatctta cataaataac taaaaaacaa aaaaccagaa aatcagtata ctgattttct
      661 ggttttacat ggtttgttca ataaaaagct gttggaactg ttaatattaa attatcgcat
      721 cgttacgaat tcttccgctc ctgttggatg gatggctact gtattgtcaa agtcttcttt
      781 agttgcaccc atcttgatag ccacggcaaa accttgcagc atctcatcga caccaacacc
      841 aatagcatgt aagccgacga ttctttcttc ttttccgaca cagatcaatt tcatttcgca
      901 tttttggcga taatcattta atgcaaagta cattggggta aagcgtgaac gataaacttt
      961 caattcattt tcgccatatt tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat
     1021 tggtggatgc gtaaagacga ctgtcggaac aagtttatag tcaagataag aatctttctt
     1081 tccattaaat aaacgttctg ataaacgtct accagctgcg attgcaacag gagtcagatc
     1141 taatttaccg atcacatcac caacagcaaa aatatgttca tccgttgtat tttggtattt
     1201 atctactttg ataaaccctt ttttgtccaa ttccacattt gtattttcca gacctaattt
     1261 ttcggtattc ggtacacgtc caccggcaaa aagtacacaa tcaccagtta aggtttcacc
     1321 attttcaaat agcaccgtat attcgtcatt gtttttagta atttctttag caatatgatg
     1381 tgcgtaagtt tttattccag cttcttggta catttggatc aaattgtccg aaagcatttt
     1441 atcaaacgtt ctaagtggtc gttcttttcg atatgcccac atgacttcac tacctaaacc
     1501 acgaaataca cctgaaagtt cagccgcaat gtatccagca cctaaaacga ttgctctctt
     1561 aggtaactct gttaattcaa aaaatccatt tgaatcaatt gcgtattctc caccaggaat
     1621 ggtcatttta cttgcacggc ctccagtagc gatcaagatt ttaggagcac gataggtttc
     1681 accgtttact tcgatcgttt gactatcgat aaaagtagcg taacctttta tagcctcgac
     1741 atgattgcta tctaatcctc tttgataagc accgtgtaga aaatcaatat atttttctcg
     1801 tctttctact agaccggcga aatcaacatt ttttaaatct gcttggatac cgtaacttga
     1861 ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc cacataactt ttttaggtac
     1921 acaaccgacg ttgacacatg ttccgcccaa ttcattcgct tcaatcagca aaacttttgc
     1981 gccatgcatc cctgctctgt ttgctgaagc aataccgcca gagccgccac caattacgat
     2041 gtaatcatat atttgcattt tgttcctcct tattgaagag atcaatcaaa acctttattt
     2101 aacgatcctt tttctacaga aaacaagtgt aacacttttc ttgaattttg tatgcgaaga
     2161 tgcttaaaac agggatatat cagctttttc cttatttacg ttttcggatc atatctccag
     2221 gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat tggatttggt gctcggtcaa
     2281 tggattcgcc ttcttcatta tgcataactt caacggtttg actgaaatga ctgaatcctg
     2341 gaccataaaa ttcgatttca tctcctacag aaaacaagtt tctctgacgt atcgtagcaa
     2401 tctgtgttgc ctcatcataa gataatactt caccgacaaa tttatatact gggattttac
     2461 gacgttcacc gaataattgt tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta
     2521 attctcgttg tgctactttc cataactcgt cgatccattc ttgcttgcag acatagtttt
     2581 caggatcttc catataactg tctacagccg ctttatagac atttgacacc gtagatacat
     2641 aatggatcga tttcattctt ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt
     2701 ctggaatatg ttcgatcatt gacatatcca ctgcactcat cgagaattct tcttccactt
     2761 cgcctgtatc agttaaactg tttcgttctt gaccaaaagg catatcgaac aatccatact
     2821 tccagcgaca agattgagaa catccgccac ggtttgcatc ccgcatcgac atatggttgg
     2881 acaatgtgca tcgaccagaa taagagatac acatggcacc atggataaat gcttcgattt
     2941 cggtatccgt atttttacga atctctgcca cttcctccat tgaaacttca cgagctaaaa
     3001 cgacacgttc caacccttct tctttccaga attccaatgt ctcaaaatta gttgctgaag
     3061 cttgagtgga taaatggatc ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag
     3121 ctggatcaga aacaatcact gcagaaatac cgacatcacg caaactgcgg aaaaattccc
     3181 ctgctccatc ttcatttcct tcatgggtga ccatattagc agcaacgtat accttcgcat
     3241 catgttcttt tgcaaaagca actccttcag ccatttcttc ataagtaaaa ttacctgcac
     3301 ggcttcttaa accatatgca ttcccgccaa tatatactgc atctgctcca taatggatcg
     3361 cggtttttaa cttttccagt gttcctgctg gcgctaggat ttctggtcgt ttcaattttc
     3421 tttttactgt catttctttc atcatactcc ttatttgata gcagaaggat caatataaaa
     3481 gaatcctgta tctaattcac ggttttcagg atgcaattta ctgatttcgc tagtcatttt
     3541 ttccgctttt tcttgatccc agctaccttc taaaatttgt ttttttgctt cggcgaatag
     3601 tcgagtgatg tttacgaatt tttcgcctgg agtaaataat ccgtccaatt tccatgtaca
     3661 gtaatgatgc tgataaagtt cattcaattc attcgagaga ttcagatcat tgctggcaaa
     3721 aatatgtgtg ccatgactat cttcataaat agaataatgt gtctcttctt ttttaggttc
     3781 cgagataaaa agacctcttt cacgatcttt ttgttcgtct tgtttcgtat agttatagta
     3841 attctgtagc agtggacgtt tggattgatg gatgcatgta gcaccataca caagggtttc
     3901 tacaggaata tcgagttttt cttccatcgc cttcatctct tcaaaaggaa cttcacgagc
     3961 aagtactgct ccaactgcgc cttttttcgc ccagaaattg atttgacgtg cgctcgttac
     4021 taatgtttca gcatcataaa tatacggtat acgcaattca ggattttttt tcatcgtata
     4081 aataatacct gtatcaccaa ctgtgatcac atctacgttt atttctttta aaaaagccaa
     4141 atattcaggt acatttttca ttttttcagg atgaatgatc ccatttacag caatattcgc
     4201 ttttttacca tacttgtgtg ctaattctac caattgtcgc tgttcttctc gtgtaaatga
     4261 agcaggcaga cgcaatccaa attgctcttc accgaaatat agtgtatctg cacctgcagc
     4321 aagcaactgt tctgcttgtt caatagtttc tactgtggtt atgagttcga tcataatttt
     4381 ctcctcctac aacttcataa tagtagcata gctctaaaaa ctttccaata cttcttctat
     4441 ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa aataaagttc gatatattct
     4501 gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta
     4561 ctattataaa aattcagcta cacaggccaa gtaactatag ggaacctact gtttcctttt
     4621 taggtccgcg aacattcaat gtgatcttac cttttgaagc acctactgtt acgtggtcac
     4681 ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg atcttcaatt tctttttgaa
     4741 gtgcgcgacg gattggacgt gcaccatatt caggatcaaa tccagctttc ccgatcacat
     4801 cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg
     4861 ctttgctcat gatttggacg attgtatgga tttcatcttt actcaatgaa tggaagacga
     4921 cagtctcatc gattcgattc aagaattcag gtcggaaagc tttctttaac tcttctaaga
     4981 tacgtttttg catggctttg tgatctttag taacatcttg gacattgaat ccgacatttt
     5041 tttcttctcg aatttgtgtc gctccgatat ttgaagtcat aatcatgatt gtatttctaa
     5101 agtccacttt tctaccttta gaatctgtta aatgaccatc atccaatact tgcagaagta
     5161 aattgaaaac gtctggatgt gctttctcca cttcatctaa gagaatcact gaatatggtt
     5221 tttgacgaat tttttctgtc aactgtccac cttcatcata ccctacgtat cctggaggag
     5281 acccgattaa tcggcttgta ctgtattttt ccataaattc tgacatatca acacggatca
     5341 gggcatcttc acttccaaac atgacttctg aaagtgcttt ggccaattct gtttttccga
     5401 caccagttgg tccaaggaac atgaatgaac caattggacg atcaggatct ttcaatccgc
     5461 ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc ctcatcttga ccaacgacac
     5521 gttcatgcag aagtccttcc agttctaaca agcgttcgct ttcttttttc tccaactgct
     5581 gtagagggac accagtccac tccgatacaa ctgtagcaac atcttcttct gttacacgat
     5641 cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac tttttgcagc tttttacgta
     5701 gtgctttttc ttgtctgcgt aattgagcag cattttcaaa gtcttgtttt tggatcgctg
     5761 cttccttttc ttgtactagt tcatcgattt ctagtttgat cacagttgat tttgttaagt
     5821 gatcggtttg atccaaacgg actttcgctg ctgattcatc gatcaaatca attgctttat
     5881 cgggtagttg acgagaagtg atataacgaa cagaaagatt gactgctgct cgtacagctt
     5941 catctgtgat ctccactcca tggtgctctt catatcttgt acgtaatcct tgaagaatca
     6001 cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg
     6061 cagaatcttt ttcgatatat ttttgatatt catctagggt cgttgctccg atcgtttgta
     6121 attctcctcg agctaacgcc ggtttcaaga tattcgaagc atcaattgct ccttctgctc
     6181 cacctgcacc aattaaggta tgcaattcat caatgaataa gattacttga ccatcattat
     6241 agatttcgtc gatgaccttt ttcattcgat cttcaaattc accgcgatat ttcgtaccag
     6301 caaccaaaga tcccatatca agcatcatca ggcgcttttc ttgcatgtct tctggcactt
     6361 cacctaaaat gattttttga gctaaccctt cagcaatggc tgttttgcct acaccaggtt
     6421 cacccaccag tacagggtta ttctttgtcc gtcggctaag aatctggata agccgtttca
     6481 cttctctcga acgaccaaca actgggtcca gcttgttttc ttttgctaat ttcgtcaaat
     6541 cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat
     6601 ttacatttct acgacgattc attccttttc cagcagaatc accagctagc cccatttttt
     6661 gtttcagaac ttgacgggct tttgaaaggc tgatacctaa gttgatcatg attcgagtag
     6721 ccatgatact ttcatctttt aataagctca tcaaaatatg ttctgttcca actttttgag
     6781 aaccagtttt cttcacttcg ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat
     6841 atggaagaaa gactccttta gggtaagaac ggataccacc ataaccaatc agatgttcaa
     6901 tttcttcata aatttccgtt tcattcaaat ccatttcacg taatactttt ccagcaattc
     6961 cattttgctc gaccactaag gctagtaaaa catgttctga actaactgat tgatgtttca
     7021 accgttttgc ttcttcttgt gcaatttcta atactgcttg ggctctttca gtaaaaagtt
     7081 cttccatcta atctcacttc ccatcttcat aacttaaacg ttccagaaat gctcggagaa
     7141 tccgagcacg tgtatgatct tcatattcat tgaaattcaa tgtgtttttt gcaattgcac
     7201 tcaacatcaa gtttccttct ttttttgtaa ttatttggtc ttcataaagt ttttgaataa
     7261 tagcaaaagc atttttctca ctgattgtat catcaaataa ctgatttatc tgctcaagta
     7321 attgtttttt atctgaaatc cttacttttg caattcgtat ataaccacca ccaccacgtt
     7381 tgctttctac tagataacca cgttggatgg taaaacgggt attaatcaca tagttgattt
     7441 gtgaaggaac acagttaaag aggtctgcca tttcaatcct tcgaatctcg atcatttcac
     7501 tattttccag aattttcttt atatatgatt caattaaatc cgaggtattc tgatgtgcca
     7561 tcttattcat tccctttgac taatattgac cttaattata acgaatttga taatagaaag
     7621 aaagaagaac gctttttgtt aaataaatcc tttagaatca acaaaaaaaa atacggaata
     7681 tactattccg tatcgacgcg cccaagagga gtcgaacccc taaccttttg atccgtagtc
     7741 aaa
//
LOCUS       DASJON010000136         7727 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJON010000136 DASJON010000000
VERSION     DASJON010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.138"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="U3989_002126"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="U3989_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN1715563.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="U3989_002127"
     CDS             complement(2020..2922)
                     /locus_tag="U3989_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1715564.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="U3989_002128"
     CDS             complement(2919..3611)
                     /locus_tag="U3989_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN1715565.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="U3989_002129"
     CDS             complement(3654..4295)
                     /locus_tag="U3989_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1715566.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="U3989_002130"
     CDS             complement(4333..4647)
                     /locus_tag="U3989_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715567.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="U3989_002131"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="U3989_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="U3989_002132"
     CDS             complement(5984..6916)
                     /locus_tag="U3989_002132"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN1715568.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="U3989_002133"
     CDS             complement(6913..7689)
                     /locus_tag="U3989_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN1715569.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
BASE COUNT         2237 a         1712 c         1356 g         2422 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtctt
//
LOCUS       DASJON010000137         7693 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJON010000137 DASJON010000000
VERSION     DASJON010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.139"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(46..366)
                     /locus_tag="U3989_002134"
     CDS             complement(46..366)
                     /locus_tag="U3989_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1715570.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="U3989_002135"
     CDS             complement(514..2748)
                     /locus_tag="U3989_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1715571.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="U3989_002136"
     CDS             3227..3493
                     /locus_tag="U3989_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN1715572.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="U3989_002137"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="U3989_002137"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN1715573.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="U3989_002138"
     CDS             5384..6430
                     /locus_tag="U3989_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1715574.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="U3989_002139"
     CDS             6447..7670
                     /locus_tag="U3989_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1715575.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
BASE COUNT         2415 a         1390 c         1559 g         2329 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaa
//
LOCUS       DASJON010000138         7619 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJON010000138 DASJON010000000
VERSION     DASJON010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.140"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(102..626)
                     /locus_tag="U3989_002140"
     CDS             complement(102..626)
                     /locus_tag="U3989_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715576.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="U3989_002141"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="U3989_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN1715577.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1215..2195)
                     /gene="pta"
                     /locus_tag="U3989_002142"
     CDS             complement(1215..2195)
                     /gene="pta"
                     /locus_tag="U3989_002142"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN1715578.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2348..3028)
                     /locus_tag="U3989_002143"
     CDS             complement(2348..3028)
                     /locus_tag="U3989_002143"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1715579.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3154..4023
                     /locus_tag="U3989_002144"
     CDS             3154..4023
                     /locus_tag="U3989_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1715580.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4049..4564
                     /locus_tag="U3989_002145"
     CDS             4049..4564
                     /locus_tag="U3989_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715581.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4611..5204)
                     /locus_tag="U3989_002146"
     CDS             complement(4611..5204)
                     /locus_tag="U3989_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715582.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5229..5408)
                     /locus_tag="U3989_002147"
     CDS             complement(5229..5408)
                     /locus_tag="U3989_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715583.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5485..5994)
                     /locus_tag="U3989_002148"
     CDS             complement(5485..5994)
                     /locus_tag="U3989_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715584.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6090..6470)
                     /locus_tag="U3989_002149"
     CDS             complement(6090..6470)
                     /locus_tag="U3989_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1715585.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6800..7549
                     /locus_tag="U3989_002150"
     CDS             6800..7549
                     /locus_tag="U3989_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN1715586.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2167 a         1580 c         1354 g         2518 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     1201 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     1261 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     1321 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     1381 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     1441 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     1501 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     1561 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     1621 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     1681 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     1741 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     1801 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     1861 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     1921 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     1981 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     2041 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     2101 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     2161 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     2221 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     2281 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     2341 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     2401 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     2461 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     2521 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     2581 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     2641 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     2701 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     2761 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     2821 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     2881 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     2941 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     3001 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     3061 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     3121 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     3181 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     3241 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     3301 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     3361 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     3421 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     3481 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     3541 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
     3601 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
     3661 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
     3721 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
     3781 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
     3841 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
     3901 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
     3961 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
     4021 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
     4081 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
     4141 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
     4201 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
     4261 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
     4321 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
     4381 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
     4441 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
     4501 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
     4561 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
     4621 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
     4681 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
     4741 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
     4801 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
     4861 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
     4921 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
     4981 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
     5041 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
     5101 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
     5161 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
     5221 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
     5281 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
     5341 tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt
     5401 ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta
     5461 aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc
     5521 atctattaat cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt
     5581 cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt
     5641 gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg
     5701 tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg
     5761 gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc
     5821 agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat
     5881 ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat
     5941 accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca
     6001 ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa
     6061 acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct
     6121 ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat
     6181 tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca
     6241 agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca
     6301 aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg
     6361 cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc
     6421 tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc
     6481 ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga
     6541 ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag
     6601 gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt
     6661 caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt
     6721 ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg
     6781 tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg
     6841 ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct
     6901 tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt
     6961 attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc
     7021 tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca
     7081 gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa
     7141 tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac
     7201 tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc
     7261 gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg
     7321 ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta
     7381 tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt
     7441 gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg
     7501 tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaactgagc
     7561 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaa
//
LOCUS       DASJON010000139         7584 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJON010000139 DASJON010000000
VERSION     DASJON010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.141"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..2757)
                     /locus_tag="U3989_002151"
     CDS             complement(55..2757)
                     /locus_tag="U3989_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1715587.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2770..4059)
                     /locus_tag="U3989_002152"
     CDS             complement(2770..4059)
                     /locus_tag="U3989_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN1715588.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4209..5255
                     /locus_tag="U3989_002153"
     CDS             4209..5255
                     /locus_tag="U3989_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1715589.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5257..7410
                     /locus_tag="U3989_002154"
     CDS             5257..7410
                     /locus_tag="U3989_002154"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN1715590.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2132 a         1554 c         1379 g         2519 t
ORIGIN      
        1 ctaaaatttc tccaccatct gaaaccgcac acacaaattt tgttccgcct gcttctattc
       61 cgccatacat tcgttttcct cctcgaatcc gattgtccga atctcataag gttggattat
      121 tgggacaacc gcttgcttcg ttggttcttc aagcagattc aatatgacag tttttccatt
      181 gtctttagtt atttccaatt tttctaaatg actagacata ttgaaacctc ttactactac
      241 tttatcacta aacttgctgc gtttcaaagc tgtaattgca aacgtttcac tttttatcgt
      301 taaatattgt tgttttgaaa tcaatgttcc ttcatgatgt ttgatttgtt gagtggaaaa
      361 aggaatctgt gctgcgtatg catgcaaata agtagagaat ttttcttctg gtccatgtag
      421 ttcaatgctg taattgaacc gatgctcacc tagacactgt gcttctggtg ttggaaaata
      481 tccccaatcg cctagttctc ccacactgcg aagtagcgtc actgcgattt gtccgtcttc
      541 tgttacttca tattcattca aaccaaagtt tccgactgtt acaccgtact cttcatcatg
      601 aatattgaca aatgcttgct gatgttgcgg attcgtgggg ttttcccaac ttttagacac
      661 atggtttggg cgcttcacca cttcaaaaat actatctgct tcgtgatgtt ccacatgtaa
      721 ctttgtagga aacaagacac gtaagcgatg atctttcatc tgattgtcga gagtcgtttc
      781 aaagtcgatt tttttactat cttttctgat tgtcatgatt gtttttattt gaaggattcg
      841 tttttctttg gatcgttctg cttttcgata acgaaattcc acaaccatct gctgttcttt
      901 ttctaaacgc tcgtctgcag aaacagggat ttccatttcc tgtagaatac ttatttttgc
      961 tatttccggt gtatctacta atacttttac ttcagagttt tctttgttac tggataggat
     1021 tggctgatca cagaaaggtt gtttaaagat atactcattt cctatatcgc cgatatcttc
     1081 aaaaatatgc agattattca actttcgttt gtttttgcga tcttccattg taatggtacc
     1141 atttttttca atcgtcaaat gtagagggcc gttttcgatg attcgtccac tctgatgaat
     1201 cattgtttcc ttttcttctg ctttcgcttc cccttctaca agcgcgaatg tttcccaaga
     1261 aaatgctggc atgttttcta atggtaaagt cactttgatc attcttgcca tatatggcac
     1321 acggaaacgg tctttcggca aatcataacc aaattggaca gtttcttcag ataaaatagc
     1381 tggtatagtg gccccacttt ttgtttctac atgatactta cgtttaggca aattttctaa
     1441 ctcttgatat agttgttcag gtattccttc agcgaatctt tttctttcta attcgatggt
     1501 tatttctgct tcaccagttt tatccattcc tgcagtattt acaatgacaa atggaaagct
     1561 gtcttttggg aatccggtcg tatcgattgc tgcggtcagc tgttctaagg aatcttgtgc
     1621 aagatacttt ccaacttcat ctgctttttc aaaacgaggt atcatttccc gatggacaga
     1681 atcgactgaa caaccgcaaa tactgtcgtg aggatgattt tgcattaatg tcttccaagc
     1741 ataatctaat tgatcatggg gatagtttcc actaacttca taagccatcg tcgcaagtgg
     1801 ttctgtaata ttttctagct gtcgctggac cttggtattc cattgtttta agtaaacccg
     1861 agcagatgct gtattggcta aagtatacca gccatccgtt tcttgacttg tcaattctcc
     1921 ttctactgag cctaagtctt ctggcacatc tttttgaact gcttccagat agtcagtaaa
     1981 attagaatga ataaactcgt aatctggaaa cagttcattt gcgagatgga ttgcttttga
     2041 tatatctttt tgtaccggtt gatgatcaac accattcatc atcaataagt ggttcgtaga
     2101 ggcatattgt tctgcatccg ctaatttttg cttccaaaag gcaagagctg cttctttttc
     2161 tactggaatc tcattcccgt tgctatacca attggcaaat agcagaccaa agattgctgt
     2221 ttgatcaggt cctttccacc acatctctga gtattgggaa gaatagtttt ctgcttccaa
     2281 aacttgatta tcaaagccaa ttggttttac accacggcca aacgctgctg ctgaaatacc
     2341 agcttgtttc attaattgag gcgtctgtcc catatttcca aatgtatcgg gaaaatagcc
     2401 taacatgaca ggggttcccc atttacggct ttcttccatg ccaatcagca tatttctcac
     2461 gttcgattcc gagctgataa gaaaatcatc ttgcaagata taaaatggtc caattctcaa
     2521 ttttccttca ttgattgctt gttggattgc ctgtcttttt tctggcctta cttgaagata
     2581 gtcatctaaa ataatcgttt gcccatccaa atgaaaactg ttgaaggacg gatcaacttc
     2641 aaatagctct agtagatcgt ctatcaattc aatcagtcgc atgtgatgct gttcgtaagc
     2701 catgtaccat tcacgatccc agtggctatg agaaatgata tatactttct ttttcattta
     2761 cgatacctcc tacttttcta ctctaatatc aaagtaatcc atgactaatt cgcaaaacat
     2821 catatttgcc caagaaaacc attctctcgt ataattttca ggattgttca catcgaatcc
     2881 ttcgtgcata aggtgtgttc ctgcgtcagt tgctacaagt aaattcaata tccgctcttt
     2941 ttcagatttg tctgacgttg tcattccttc catagctaat gcaatcggcc aaacgtattt
     3001 ttctggcgta tgggaggatc caattccttt tgcgtacttt ccttcataga aatatggatt
     3061 ttctttactc aacaatgttc ttcttgtttg cagataatct tcctcttgtt cagaacaata
     3121 tcccaagtaa ggtgcggcga tcagattggg cacattactg tcatccatga ctgtggcgtt
     3181 tcccattcca tctacttcat atgcataaat cgtttgccct ttttgattaa ccgttcgtcc
     3241 gtgattctga atcccctctt ggatctcttt cttcaatttc tgtgtttgct gtacaatttc
     3301 tttatcatcg agtatcgttt gaaaaatttc ttccacataa cctaacacga ccaccgcaaa
     3361 catattggat ggtaccagat atccataacg gcaagtatca tcgctaggac ggaaaccaga
     3421 ccatgtcatt cctgtaaaag ctgtttttgc acctcgcccg tcattgatca gcgtatcttc
     3481 ttttcttgtc gtgtctcgta caaatgtata aggagagtgt tcatgatctt gctctgtttt
     3541 gaacacgtcc aagatttttt tgataccttc aataaaagat tgattgaatt gatcagtttt
     3601 tcctgtattt ttgtaaagaa gataggccaa ctgtattggg tagcataaag aatcaatttc
     3661 atattttctt tcccagatcc aatcgttcat ttttgtatga tctgtttggt ggccagcacc
     3721 gttagcttct tcgttgaagg cattcgcata tggatcgata ttgatataaa aaaattgctt
     3781 tttgactaac ccgctgatca tgtcagctaa ttcctcatct tcttttgcaa tcactaaata
     3841 aggacgaact tgtgcagtag agtctcttaa ccacatcgct ggaatgtctc ccgtcaataa
     3901 aaaggtggtt ccgtcatttt gctttttgac tgttgtcaac agagtattgg caaatgctgc
     3961 tgtaaaattc tttgcccagt ccgcatggat atccccacat ttttgtgtga tatcttccat
     4021 aaatttctgc accgattttg gtatatcttt ataagccact aataaaacct tctttctatt
     4081 tgatatatct ttaaagatta tacttcatac agccaattat gtctacgctt tcaaaaaata
     4141 tctttttatt tactttttca tccattatgg tattatttga atatgatata tcattaagag
     4201 gtgtttatat gaatcaacca ttgtaccaga caatatttaa tgatttgcaa acggctattt
     4261 taactggtga attacctgta gacagccaac taccaactga aaaagaactt tcacgtacct
     4321 ataaagtaag cagaatcact tccaaacgtg cattgactga attagagcaa ttaggactta
     4381 tttatcgcgt tcgcgggaaa ggcagtttcg ttaaatccgc ttctccggaa cagattcttg
     4441 cttctcctac aaaaacacga cggattctgt ttcttcttcc atatgtcgct gatttgtctg
     4501 ttggtgattt tagcaaggga ttgaaccctg tgatgcaaga taaacagatt gatgtcatga
     4561 tgacgacgct tgactttcta caaaataaaa cagcaaaaga gctaatcaat gaatttgacg
     4621 gattgattta ttatgctctt gatacagaac aacatcttga cttacttttt gagctttcat
     4681 tgaaagaatt ccctgtcgtc atattagata aaaaaatcta tgagttacct tttcctaccg
     4741 ttctttccga taatttccaa ggcggtgcgc ttgctgcctc ttatttgatc gaacagggac
     4801 attcaaaaat cgcttatttg tttggtacgc agactcatcc acaatccgtt cgacagcgtt
     4861 accttggtta ccttgaagca ttggacaaag ctggtttgac tttccacact tcactggatg
     4921 acaaaagcgc gacattagat gaattgcttt cttatatcac taagtatgaa gtaactgctt
     4981 ttgtttgtga aaacgatgtt gtcgctatcc agaccatgaa tctcttgaga cagcatggtt
     5041 atcatatccc tgaagatttt tcaattatcg gatttgatga tatccaagca gcagctcttg
     5101 ttgacccgcc actgacaacc atcgcacaag attttgaaca gttaggtaag cttgcgggag
     5161 atgctttggt cacttggtta gagacaaaag atcgaccatc cgatagtaaa tttccagttt
     5221 cattcatcaa aagacaatca acaaaggaga aataaaatga atatcggtca aatagatact
     5281 agacatgcaa cagcgaatca gccaaatttc tctaatggga actgccagcc ttatactggc
     5341 gttccttttg ggatgaatta ttttgcaccg caaactacgg atcaaaacgg cagctggtgg
     5401 tttcatcctg atgaccgcgt ttttcaaggc taccgtctta cccatcaacc aagtccttgg
     5461 atgggtgatt tcagtcatat gctgtttacg ccgatcaatg gaaaactaca ggagaacacc
     5521 cttttccatg ctcaaagttc ttaccgccca gaagaaagta tattttgccc gactcatctg
     5581 tcgatctgtc aattacgtta tggcatctgt tctacattga tacctagcat gtatggcgga
     5641 atcttatcta ttgattacag caaaaatgac agcggtttac ttttatcctt tccgggacgc
     5701 catcagctct ttgttataga tccttacaca gtggaaggaa aaatcacaca ttttgctggt
     5761 tcagaagatc ctgatttttc tttttatttt atattgagat ttgaatatcc tttaacaatt
     5821 gatgagttag ctgtttccaa caaaaatagc gactcgatcc cgttgtattt tgaacagaca
     5881 aaaaaacaaa ctatccgatt tgggacatct tttatcagct cggaacaagc agcttataac
     5941 ctggaactag aacaaaacca aagcaaagag acttaccttg ctcacagtca agaacaatgg
     6001 cagtcctatt ttgatcgtat acagctcaca caccatgatc aacaacaatt gcggacgttt
     6061 tatcataatt tatatcgggt gtttttattt ccacaaacat tttatgaaat tgatcctagt
     6121 gggcaaaaaa tccactatga tacgactagt cgatctataa aacctggtgt tctctataca
     6181 aataatgggt tttgggatac gtataaaacg gtttatcctc tttattcttt gattgctgta
     6241 gaaaaatacg aagaaatgct ggaaggtttt ttgaacagct atagagaaag tggctatttg
     6301 ccaaaatggc tgtctcctga tgaacgtgga cttatgcctg ggacattgat cgatgcggtc
     6361 attgcagatg ctgctgtaaa agatatccga cgagacttga tgcctgaatt tctaacggca
     6421 atggaaaaag gtgctacggt tcaaagcaaa catccaaatt atggacgcca aggaacagaa
     6481 gattacttga catatggcta tgttcctaat cactaccacg aatcagtcaa tcacacatta
     6541 gactatgctt atagcgattt ttgtattagc caagttgcca aggtactaga agaagaggaa
     6601 atgacgaaga aatatcagca gcaatcattg aactatcaaa atatctttga ctatgagtca
     6661 ggatttatgc gttcaaaaga taaagatggc aagttcagag aaccattttc agctaatcgt
     6721 tggggacaag attatgcaga aggaagtgct tggcaaagca gctttgctgt ttatcacgat
     6781 ttttcaggac tgatcggaaa atatggtggc agtgagagat ataaagaaaa actcattgag
     6841 ctgtgcaatc agcccccaac gtttgaccca ggaggatatg gttttgagat ccacgaaatg
     6901 agtgagatgg ccgcgataga atttggacaa ttagctattt caaaccagcc aagttttcac
     6961 tatccttact tgttcagtta tataggacaa cctgaaatgg cacagccact gcttaaacag
     7021 ttgatgactc agaccttcga cgattctcca atgggttacc ctggagatga ggacaatgga
     7081 agtatggccg cttggtatat cttcaatagt ctgggatttt atcctgtcac gcctggaacc
     7141 ggtgaatatg ttatcggtat gccattaatt gacaaggctg ttttgctttt gtcaaatgga
     7201 aaggaattga cgattgaagc aacgcctaat cggccgcagc aacaatttat ccataaaatc
     7261 aaacggatgg acaaagaata tacgaaatta tattttaccc atcaagattt gctgaaaggc
     7321 ggcacgatct cttaccaatt agggatcatc cctcacccta agaaatatca agcaaaagat
     7381 ttgccttttt ccttatcaaa ctataattaa caagaggttg tgaaaaagct gtcctgcatc
     7441 aagtaataag aacagccaag caaaaataga tcctcatatt tttcgtttgg ttggtcttat
     7501 taccgcagat gcaagctttt gaacaccgtt tattgagagg ttgtgaaatc agcgttctga
     7561 cctgagtatt agagagacaa atgg
//
LOCUS       DASJON010000140         7488 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJON010000140 DASJON010000000
VERSION     DASJON010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.142"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            58..1199
                     /locus_tag="U3989_002155"
     CDS             join(58..286,286..1199)
                     /locus_tag="U3989_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1715591.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1434..1886
                     /locus_tag="U3989_002156"
     CDS             1434..1886
                     /locus_tag="U3989_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715592.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1900..2589
                     /locus_tag="U3989_002157"
     CDS             1900..2589
                     /locus_tag="U3989_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715593.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2714..5065
                     /locus_tag="U3989_002158"
     CDS             2714..5065
                     /locus_tag="U3989_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN1715594.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5145..5540
                     /locus_tag="U3989_002159"
     CDS             5145..5540
                     /locus_tag="U3989_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715595.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5865..6482
                     /locus_tag="U3989_002160"
     CDS             5865..6482
                     /locus_tag="U3989_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1715596.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6523..6822
                     /locus_tag="U3989_002161"
     CDS             6523..6822
                     /locus_tag="U3989_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN1715597.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6856..7023
                     /locus_tag="U3989_002162"
     CDS             6856..7023
                     /locus_tag="U3989_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1715598.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7068..>7488)
                     /locus_tag="U3989_002163"
     CDS             complement(7068..>7488)
                     /locus_tag="U3989_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1715599.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2584 a         1129 c         1498 g         2277 t
ORIGIN      
        1 ttaaaacttt gcaacagaac cgtagaatct aactactaag aaaaggacgt gtccacgatg
       61 gcaaaagcca agaaaaaatt tgatgaagac tttaagaaga tgatcttaga tttaaaccag
      121 tctggccaat cggtcgaaga actggcagcg caatatggta tcgctacaca aacgatttat
      181 cgttggaaga agcttcatac aaagaatgta gcaaccggca tgactgaggc agaaatccta
      241 gcgatgaaaa aggaaatggc ccgcatgcag gaggaaaaca ctatcctaaa aaaggcttta
      301 accatattcg ctcaaaagta aaaatgagtg atgtcttcga atttattcaa tcaaatgttc
      361 atgatcatga cgtaaaacaa atgtgtaccg ttttggaagc accgaaatcc agctactatg
      421 actggaagaa aaagaaacct tccaagcgcc aatcagaaaa caaacgctta aaccgcttga
      481 tttcaggaat ctactttgaa aacaagggta tctatggtgc gcctaaaatt cataagattc
      541 ttgttggccg tggcgagacc ttgtccttaa agaaagtaca aatactgatg agaaaattag
      601 gcttgcgttc agtcaccctc aagaagtaca agcctggcaa acaagcgaaa gttaaaagtg
      661 aaggacgtaa aaacctgtta aaccaagatt ttaccactac gtcgatcaat caaaaatggg
      721 tgacagatat tacctatatt cataccctaa aagatggctg gacctacctc tccactattc
      781 aagacctcca cacaaagaaa gtcatcggtt ggaagtttgg caagcagatg acgaaagaat
      841 tggttatcga aacccttgac aatgcgctct taaaccaaac accctccgag aacttgatca
      901 tccactcaga tttgggttct cagtacacta gcgaagccta tgaggcaaag ctgaatgaat
      961 tgaacattcg gcactccttc agtcgtaagg gctgtcccta cgataatgcc ggtatcgaat
     1021 cgtttcacgc ctcactcaaa aaagaagaag tctatccatc aaaagcgtat gaaaactttg
     1081 aagctgctta tttagcgtta tttcaataca ttgaaggatt ttacaatcgc aagcgaatcc
     1141 atagtagtat caactattta acaccaaatc agatggagga gttggcattg caagcttagt
     1201 tcctagctac cacttacatt tttttggcga gatttccaca gggtttcaac ttcatcgctt
     1261 cgatcagcgg tcaaacggtt ttggtttgac cgctggtcga aacgatgaga tactcacccg
     1321 ctggaatctc gaaaaaattc tcacaaatcg tgtccgaggt attgactcaa atccaccaat
     1381 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
     1441 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
     1501 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
     1561 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
     1621 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
     1681 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
     1741 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
     1801 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
     1861 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
     1921 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
     1981 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
     2041 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
     2101 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
     2161 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
     2221 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
     2281 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
     2341 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     2401 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     2461 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     2521 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     2581 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     2641 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     2701 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     2761 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     2821 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     2881 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     2941 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     3001 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     3061 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     3121 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     3181 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     3241 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     3301 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     3361 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     3421 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     3481 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     3541 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     3601 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     3661 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     3721 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     3781 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     3841 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     3901 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     3961 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     4021 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     4081 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     4141 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     4201 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     4261 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     4321 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     4381 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     4441 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     4501 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     4561 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     4621 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     4681 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     4741 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     4801 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     4861 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     4921 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     4981 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     5041 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     5101 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     5161 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     5221 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     5281 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     5341 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     5401 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     5461 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     5521 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     5581 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     5641 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     5701 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     5761 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     5821 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     5881 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     5941 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     6001 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     6061 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     6121 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     6181 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     6241 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     6301 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     6361 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     6421 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     6481 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     6541 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     6601 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     6661 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     6721 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     6781 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     6841 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     6901 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     6961 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     7021 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     7081 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     7141 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     7201 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     7261 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     7321 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     7381 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     7441 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgat
//
LOCUS       DASJON010000141         7454 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJON010000141 DASJON010000000
VERSION     DASJON010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.143"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            273..1751
                     /locus_tag="U3989_002164"
     CDS             273..1751
                     /locus_tag="U3989_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715600.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2028..4088
                     /locus_tag="U3989_002165"
     CDS             2028..4088
                     /locus_tag="U3989_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN1715601.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4171..4740
                     /locus_tag="U3989_002166"
     CDS             4171..4740
                     /locus_tag="U3989_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715602.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4915..5721)
                     /locus_tag="U3989_002167"
     CDS             complement(4915..5721)
                     /locus_tag="U3989_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715603.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5859..7373
                     /locus_tag="U3989_002168"
     CDS             5859..7373
                     /locus_tag="U3989_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN1715604.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2674 a         1226 c         1452 g         2102 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1861 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
     1921 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
     1981 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
     2041 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
     2101 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
     2161 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
     2221 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
     2281 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
     2341 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
     2401 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
     2461 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
     2521 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
     2581 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
     2641 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
     2701 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
     2761 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
     2821 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     2881 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     2941 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     3001 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     3061 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     3121 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     3181 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     3241 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     3301 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     3361 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     3421 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     3481 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     3541 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     3601 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     3661 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     3721 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     3781 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     3841 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     3901 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     3961 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     4021 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     4081 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     4141 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     4201 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     4261 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     4321 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     4381 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     4441 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     4501 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     4561 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     4621 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     4681 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     4741 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     4801 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     4861 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     4921 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     4981 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     5041 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     5101 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     5161 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     5221 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     5281 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     5341 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     5401 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     5461 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     5521 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     5581 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     5641 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     5701 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     5761 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     5821 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     5881 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     5941 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     6001 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     6061 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     6121 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     6181 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     6241 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     6301 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     6361 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     6421 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     6481 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     6541 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     6601 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     6661 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     6721 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     6781 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     6841 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     6901 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     6961 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     7021 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     7081 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     7141 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     7201 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     7261 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     7321 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     7381 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     7441 gaacactgtt tagt
//
LOCUS       DASJON010000142         7316 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJON010000142 DASJON010000000
VERSION     DASJON010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.144"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1339)
                     /locus_tag="U3989_002169"
     CDS             complement(<1..1339)
                     /locus_tag="U3989_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1715605.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKN"
     gene            complement(1409..2572)
                     /locus_tag="U3989_002170"
     CDS             complement(1409..2572)
                     /locus_tag="U3989_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1715606.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            2701..3024
                     /locus_tag="U3989_002171"
     CDS             2701..3024
                     /locus_tag="U3989_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715607.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(3207..4556)
                     /locus_tag="U3989_002172"
     CDS             complement(3207..4556)
                     /locus_tag="U3989_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1715608.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4625..5578)
                     /locus_tag="U3989_002173"
     CDS             complement(4625..5578)
                     /locus_tag="U3989_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN1715609.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(5680..6870)
                     /locus_tag="U3989_002174"
     CDS             complement(5680..6870)
                     /locus_tag="U3989_002174"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN1715610.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         2150 a         1550 c         1238 g         2378 t
ORIGIN      
        1 aatttttctt atttttacga gcagtacggc tttgctgtct gcgttcgatt ttgcgttctt
       61 ttttattttt ttctgcaatt gcccaatcga tttttttctt ataaccaggc ttgatctttt
      121 tctttttctt cttcacaaga ccaatcagcg tgggatctaa tacatctttt gatttttctc
      181 gtttggcacg tctattacga tcgtatgttt tgacgatttc cccgtttttg atttcttttg
      241 gttcaaatga gacgcccaat ttttctatcg catcaatggc ttgctcatca tttggcgaat
      301 ataacgtaat ggccgttcct tttaagccat tacgtccagt acgtccgaca cgatggataa
      361 agaagtctag atcttctgga atttctgcat taataacatg agagacacct tcgatatcga
      421 tccctctagc agctaaatca gtcgctacaa catattgata ctccagattc tgtacttgtc
      481 gcatcactcg ttttctctct cttggggtga tgtctccatg aatctttgct actttcaagc
      541 cttggttttt caagtaatcg gcaatttcat ccacatgctg ttttgtattg gcaaatacga
      601 ttgctaaata aggatgacca attgtcagca gttgataaat catttgattc tcatctttac
      661 cttttgttga aatcagccaa ttttcaatgt cttcagaaat aactgctttt ggtttgattt
      721 cttcaatgat tggattttcc atgtattttc gtaagaatgg acgaagtttt tctggaatag
      781 tggcagaaaa aactaaaaac tgcagctttt ctggtaatcg acttgcaatt tggtctactt
      841 catttaaaaa acccatatcc aatgtcatat ccgcttcatc gacaacaaat gcaaatgcgg
      901 tgtgaatctt cagcgcttgt tcattcatca tgtctaatat tctaccaggc gtgcctacta
      961 caacctgtgg ctgttggtga tgcagacgat ttaattgacg ctgtttgtct gtaccgccga
     1021 caaaattcga gacacggatc tccggtgtag aaaatttagc gatttgtgta gctgcttcat
     1081 agatctgtgc agctagttcc ctacttggag ctgtaataac aacttgtact tcctcttttt
     1141 ctgcattgat tttttcaaac aaaggcaaca aaaacgtatg tgtttttcct gtaccagttt
     1201 gagattgccc aatgatattt tttccttttg caattaaagg aatcaaacgt tcctggacct
     1261 ctgtgggctg tttgaagccc tttgcggtta acgcttcatt gataaacggt ttgaagcgga
     1321 atatatcaaa attattcatt cgtgcacctc ttctctttaa attatcacga ctttggcatt
     1381 ataacacaga caaagcgttt ttgctcagct aatttcgaga atcatcatcc tttgtttggc
     1441 caatcgtgct aacagtatat gtgtgactag ctttttcata aaacagaatc cgtttttttc
     1501 tcaaaaatga tagcaagata tttaaaataa aaataccttg tagtaacaag acaccgagag
     1561 aaatgaatac tgcactcatc tgcaacaccg tattcgttgt cgaattcatg atttgctgaa
     1621 gcaactgccc caaccagctt aaaccataaa aaagaaagta cagatacccg tagcggatca
     1681 tcaatgcttt taaagaaatt cgttcgcggt cttcttccac caaacggata cgcacaattt
     1741 ttttgccaag tgtccatcca ttggttataa acggtacaat cataaagtat acccctactt
     1801 ctaataaaag ccaccagata tgagaagtaa ctgcccctga atcgaacgca attttaaaga
     1861 aaatcgccgc taccaaatta ataagagata aaaatgtcca gtcgatcaaa taagcgacca
     1921 agcgtcgagt caatgagaca gtctttcctt tttcataaga aagttcatcc attcgtcttc
     1981 ttgtaggaag cataaatgta aataacggcg taacgacaaa accaatgatt ccacccaatg
     2041 tatttgtcac taaatcattc acatcaaata agcggtatgg tcttgggtag ataaaataaa
     2101 gtccacttag ctgcgtcaat tcaaaaaata gagaaaggca gaaactagca atcatcgttt
     2161 tcataaaaga aagccggaaa taatatctta aataaatgcc aaacggtatg agtaataaaa
     2221 cattaaaagc tggttccaaa aatacactct gcttcattgc cggcaggtag gtactaggtt
     2281 cagacaagac caataccgtg ttgtctctga aatgttgcca tgaagcaaat aaatgcagct
     2341 ccatctttgg ccctgtcatt tgggcaaccg tctcctttgc cggaagtggt aagatcacca
     2401 aaaaatacgc acacaataaa taaaaaacga aagaatacaa aataactgcc cttgttacta
     2461 gaaatgttcc atactttcga tattcataaa taaaaaaaac agatgagatc gctaaggcta
     2521 aaaatggaaa aacaatcagc gcaaaacgaa tcggttcgat ataacttgcc atatgactcc
     2581 tccttgattc tttgtatctc tcttatctta gcagtccttt gctgtttcaa gcaagtaacg
     2641 aggctattat ttctcatttc tttcaaagta aagtaagata aacacaaagg gggtgtcgag
     2701 atgctgaata tgtactttgt ttttggcgta cctatttttt tactgttcct ttatgctacg
     2761 atcgcttatg taagaaaaag aactacaatt cattatttag gatttattct cctgattatt
     2821 tcgggtttca tgctagtctt caatcttcag acttggcagc aagcacttct tgaaatggat
     2881 aaaatgactc cacatgcttt gtctaaagtg cttggctacc ctgtctatct gatttggctt
     2941 cccattttca tttctggttg cttggtttta ttaaatattt atcgcggcgt acgccgaata
     3001 gttcagttac gcaaatcaaa gtaaaagaca gaaatcggtt gagcccgatt tctgtctttt
     3061 actttgctat ttcatcgtag acaaaacgac atttacgtga tcaaaattaa cgagtacttt
     3121 tgacatttct tccacgtact cctcatttaa ataaaatatt ttgtttggca gagaagtgca
     3181 gttctaataa aattcgaatg ttttagctat ctacgtttta aatagagata aatcgggata
     3241 ccagcaagtg tcaatactat ccccatcgaa gctaaaaagg tctgagtgat aagcgtgctt
     3301 gatacgataa aagtccctcc aatgattgct actagtggaa taaacggata cattggaact
     3361 ttgtatggtc gaaacaaatc aggttctttt ttacgaagaa gaatgacacc aacaaagacc
     3421 atcgtataaa aaatccaaat gacaaagatc agcatatccg ttagtgtatc aaaaccaccg
     3481 atcatcatca tgccaatagc gatgactagc tctaagatac ctgcggctac aggaacttta
     3541 gttttatcat gaagtttagc aaagagcttg ctaaaaggca ggttgttttc ttttgccata
     3601 acataaggga gacgcattcc agtaagggta tagccattga tggttccata aacagaaatc
     3661 aagataccaa ctgtgaccag tcttccaccg aagccgccaa aaatcatttt tgcaacgtca
     3721 cttgccgcat tgctgtttcc tgcgactccg tcgatagatg cggttcttaa gaacaccgca
     3781 tttactaata aataaacgat catgattccg atgattccta aagaaattgc ttttggcaaa
     3841 tctttcgccg gctttttcaa ttctcccgaa atatttccta cgtgtatcca accgtcgtat
     3901 gcgaacatcg ttgctagcaa gcccgctccc aaggctgaga aaaatgaaag attttctcct
     3961 gcttggatcg gaaacagttg gaagtctact ccttcttgtc ggaacaaacc gaaaatgacg
     4021 atcacaaaca aaggaataag cttgcagaca agcgtaatcg actgaaaagc ccctccagct
     4081 ttagaaccta aaaaattgat caacaggatc gatacagcag ctgtaacagc tacgggaaca
     4141 atcattgact gggaaagacc aaatagattg acgaactgtg taccgaaaat gatcgacaag
     4201 gctgctacat ttgctggaaa ataaatcacg acttgcgccc agcccaataa aaaagctgct
     4261 gtatttccgt aaatccgttc gatatattta atcatccctc ctgtttctgg aatagcagct
     4321 gccagttcag ctcctgttag tcctgcacag acagaaatca taccaccgag aaaccaagaa
     4381 aacatatgca aactggcgga tcctgtaact tctgctacgc ttgcggcttt aaaaaatacc
     4441 cctgcaccga tcactgtgcc cataacagtc gataaagcag taaaaaatcc catcgtacgt
     4501 tttaattcat tttctatttt tacttctttt actggcgtgg cttgatattc ttccatccct
     4561 tgtccccttt cataccatag ccaatcccaa aagtataaaa aactttttgg atgtggttct
     4621 tgttttatac cgtctctacc aatttttctt tcagtttttc caacatatcc tttgtcatca
     4681 catctaagtc gtatttcggc gaaaagcccc attcctcttg cgcacaagaa acatctagtg
     4741 aatttggcca agattctgct attgactgac gtacaggatc cacatcataa tccatttcaa
     4801 aatctggtat gattttttga atagatgctt tgatctcttc cgggtcaaaa gacatcgccg
     4861 atatattgaa ggcatttcga tgaaccaatc gatcaggatc tgcattcatg agttttataa
     4921 tggcatcgat cgcatctggc atatacatca tatccattga tgtaccttga tcaatgaaac
     4981 tagtgtactt tccttctttg attgctgaat aataaatatc gactgcgtag tctgtcgtac
     5041 cgcctcctgg caaggttgca taagaaatca atccagggaa acggacacca cgagtatcta
     5101 cgccatattt cacataataa taatcacata gtagttctcc agctaccttc gttaccccat
     5161 acatggttgt aggacgctga agagtatctt gtggggtatg atcttttgga gtatttggac
     5221 caaacgcgcc gattgaactt ggcgtaaaaa atttcagctg gaattctcta gcgacttcca
     5281 atgcattgac cagcccagtc acgttcaaat tccaggctaa ttgtggtttt gcttctgcaa
     5341 cagctgacaa aagagcagcc aaatgaatca atgtgtccac ttgatattgt tctgctatgc
     5401 tgcgcatttt ttcgtaatcc attacatcta ggatctcaaa gatatcgttt ttctcaatga
     5461 cttcaggcat gcgtatatct gtcgcaatga cattttcact gcctagttct tgacgtagcc
     5521 gtgtgatcaa ctctgaacca atttgtccta aacatcctgt caccaatact tttttcatga
     5581 tgatcctccc tgatcttttt tactactatc ttcagatgat taacaatata tgcgtgaaca
     5641 tgttctctaa ttttaaatag acgaaaaaac taaatttagt taaataattt ccatttcttt
     5701 tcctattttt tgataaactg ctactgcttc atcaagcatt tccctcgtat gttccgcagt
     5761 cggcatattt ctgatcctgc ctgtcccaag aggcacagtt ggataaacga ttggtttagc
     5821 ataaatccca tgttcgatca gcttcttaga gaaagtctga gtcacttttt cgtctcccaa
     5881 aatgactgga gtaataggtg tttcagacat accgatgtta tagcctactt ttttcaattc
     5941 ttctttaaaa taattagcat tttcccaaac tttttcgaca agttccggat gttcttgcat
     6001 caaagtaatc gaagccaatg cagctgcagc tgcacctgga gttagcgaag tagaaaatag
     6061 gaatggccga cttctagctt tcaaccagtc gattagtgtt ttcgatccag ctacataacc
     6121 cccaacaaca cctatagctt tcgataacgt gcccatttgc atatcgattt tgtcgcttaa
     6181 tccaaaatgc ttgacggttc ctgcaccttt tccagttacg cctgagccgt gcgcatcatc
     6241 gacgtaggtg atcagtccat atttttcaac aatcggaata atttccggca atcgtgcaat
     6301 gtctccatcc atggagaaaa caccatctgt gatatacatg atttttttat atttcttact
     6361 ttctatcgct tcctttgctt ttttctcaag gtctttcata tcttgatgtt taattcggat
     6421 gatttttgct ccggataagc ggcaaccgtc aataatagat gcatggttta attcatccga
     6481 taagatagca tcttcttttg tcattactgc agagatagcg cccatattgc agttgaatcc
     6541 agattgaaag gcgattgctg cttctgtccc tttgaattct gcgatttttt cttctaattg
     6601 ctgatggatt ttcagcgaac cattgatcgt tcggactgct cctgctccta caccataagt
     6661 ttcggttgct tcaatacatg cttttttcaa ttcttcgcga ttggcaaatc caagataatt
     6721 gttggatgct agattgatca tcttttttcc gtctacgatg atacaagcac cattttcact
     6781 ttctagtatg tcgatcgtat tgtataaccc cttttctttc agttgatcca attctttctc
     6841 taaatacatt gttagttctt tggaagacat tgtctccacc tccatattca tctgtcttgc
     6901 agaacatacc aaacatatat cgtgtcattc atctacaaag caagtatgct atatttttga
     6961 taacgcttac actgattatg agaattattt aaccacattt tttatttaca gtatcttttt
     7021 actttcaaat gacttatata gtatgaacaa ttacctttta gataatgatc tattttactc
     7081 tacctgggca gataaataca acacaaccat tgatagaagc cctttcgtta aaatcaattg
     7141 tattctgaat atttaattta tttattgcat agaccaataa aaatacataa aatttccatc
     7201 ttcgtttatc agcaattttt aattttactc tctgaatatt tcttaaaagt tttacttgtt
     7261 gaattcgaaa aaaggactgt gacaaaagtc ttgtcacagt cctattcttc cgaata
//
LOCUS       DASJON010000143         7296 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJON010000143 DASJON010000000
VERSION     DASJON010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.145"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..384)
                     /locus_tag="U3989_002175"
     CDS             complement(<1..384)
                     /locus_tag="U3989_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715611.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="U3989_002176"
     CDS             complement(569..967)
                     /locus_tag="U3989_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN1715612.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="U3989_002177"
     CDS             complement(964..1737)
                     /locus_tag="U3989_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1715613.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="U3989_002178"
     CDS             complement(1734..2417)
                     /locus_tag="U3989_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1715614.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="U3989_002179"
     CDS             complement(2584..3471)
                     /locus_tag="U3989_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1715615.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="U3989_002180"
     CDS             complement(3468..4337)
                     /locus_tag="U3989_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1715616.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="U3989_002181"
     CDS             complement(4474..5760)
                     /locus_tag="U3989_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1715617.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..7296
                     /locus_tag="U3989_002182"
                     /pseudo
     CDS             6033..7296
                     /locus_tag="U3989_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292017.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         2280 a         1577 c         1200 g         2239 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaa
//
LOCUS       DASJON010000144         7217 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.146, whole genome shotgun sequence.
ACCESSION   DASJON010000144 DASJON010000000
VERSION     DASJON010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.146"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="U3989_002183"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="U3989_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN1715618.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /locus_tag="U3989_002184"
     CDS             complement(919..1677)
                     /locus_tag="U3989_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1715619.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="U3989_002185"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="U3989_002185"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1715620.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="U3989_002186"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="U3989_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN1715621.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="U3989_002187"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="U3989_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN1715622.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="U3989_002188"
     CDS             complement(4433..5290)
                     /locus_tag="U3989_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN1715623.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="U3989_002189"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="U3989_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1715624.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DASJON010000145         7112 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJON010000145 DASJON010000000
VERSION     DASJON010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.147"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U3989_002190"
     CDS             6..305
                     /locus_tag="U3989_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715625.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U3989_002191"
     CDS             378..1739
                     /locus_tag="U3989_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN1715626.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U3989_002192"
     CDS             1761..2021
                     /locus_tag="U3989_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715627.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U3989_002193"
     CDS             2035..2787
                     /locus_tag="U3989_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN1715628.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U3989_002194"
     CDS             complement(2881..3486)
                     /locus_tag="U3989_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1715629.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U3989_002195"
     CDS             complement(3539..4513)
                     /locus_tag="U3989_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN1715630.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U3989_002196"
     CDS             complement(4891..5610)
                     /locus_tag="U3989_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1715631.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U3989_002197"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U3989_002197"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1715632.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
BASE COUNT         2180 a         1292 c         1458 g         2182 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt ta
//
LOCUS       DASJON010000146         7069 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJON010000146 DASJON010000000
VERSION     DASJON010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.148"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(191..343)
                     /locus_tag="U3989_002198"
                     /pseudo
     CDS             complement(191..343)
                     /locus_tag="U3989_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(611..1942)
                     /locus_tag="U3989_002199"
     CDS             complement(611..1942)
                     /locus_tag="U3989_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715633.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2091..2804)
                     /locus_tag="U3989_002200"
     CDS             complement(2091..2804)
                     /locus_tag="U3989_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1715634.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2979..4403
                     /locus_tag="U3989_002201"
     CDS             2979..4403
                     /locus_tag="U3989_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1715635.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4702..5409
                     /locus_tag="U3989_002202"
     CDS             4702..5409
                     /locus_tag="U3989_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1715636.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            5396..5719
                     /locus_tag="U3989_002203"
     CDS             5396..5719
                     /locus_tag="U3989_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1715637.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5808..6845)
                     /locus_tag="U3989_002204"
     CDS             complement(5808..6845)
                     /locus_tag="U3989_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1715638.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
BASE COUNT         2222 a         1330 c         1180 g         2337 t
ORIGIN      
        1 aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa aaatagcaag taaacgataa
       61 caaccatcct aaatccacaa gaaatacagc agaccttgct ttaaaaacac gaatcatgca
      121 aaggatgaag taaagaagac ttaacgcgta cccaatatta gtcatttgac tattaggtac
      181 gcgttttgtt ttataagtca tgacaaattt ccttcgttgc tgggtagatt ttccggcatt
      241 gttgatagat tttatgatac ttttctacat tttgtaagag aggttccact gcatctgtat
      301 attggataaa attctgacaa caactttcag gtgtgtcgaa ccactctaat ctttaaagat
      361 aaatattaaa ataacgatta ataatattaa cagtctttat ttttcaccac caaaaaccta
      421 tctttgcata tactaaatag acgtatatac acagataggt ttgtcttgtg aaaaataatg
      481 attatcctta ccaaaaaatg tttgtttttc tacatgttcg tacttatcaa aaaatatgat
      541 ccttattgaa attactggct atttatattc caacgacttc ttgtgttaat ttttgttatc
      601 tcatagtaaa ttatgtttca ttttcactta atttagttgt cacgtgttcg tcaattcgta
      661 aaaacggtaa atatataaac acacttgcgg ctataattaa tatttgtaaa acagctgctc
      721 gccagtctcc ggctgttgct aaaaatcccg atataactgg tggcgtagtc caaggaattt
      781 gcactactac atgattaatt aatcccacag cagttgcaaa atatgctact gtcaaagaaa
      841 aaatcggtgc aagcacaaag ggaataatca gactgatatt aaatacaata ggcaatccaa
      901 aaataatagg ttcgcttata ttgaatatag cgggagttac cgataattta gcaaagtcct
      961 tataatcttt tctacgactg aagataaata ttgcaatcaa taaagcaatc gtatttcccg
     1021 atccacccat taccgcaaag gtatctttaa aggccatatt gataatatgt ggaatttgct
     1081 ctttgtttgc ataagctgcc atattttctt gcatattctg aatcaaaaac ggatctaaca
     1141 atgaacctga tattactgct tgatggatac caaatccaaa aaatagatta gcaatagatg
     1201 taattaacaa aaatcctggt aaaccagttg taattttact taatggctct gtaactatcg
     1261 tggtaattag cttatttata tccatattaa acaattgaat gattataaat gacaataaag
     1321 caaacgaggt aatcgtaata atgattggga ttaatacatt aaaagacttt aaaacagcgg
     1381 gaggaatatt tccggatagc tgaatctgta gtttcttatt attagaaagt ttaataaata
     1441 attctgtagc cgctaatcca acaaatatac ctacaaacat tcctgaagct cctgtatagg
     1501 atataggtat cacacccgtc acttcaacag ctttatccgc attttcagga gtaaacatca
     1561 tctgcatagg tgttaataca acaaatgtcg aaatagcaac taataccgct gcaatatgat
     1621 ttgtataatt tctgcttgca cacatatgat aagatactgc tgctacaact aataatgtag
     1681 ttatgcttaa tgtaccattg gcaattgaag taccaatttg ttgtaacgtg gtcaatgtac
     1741 tagtatcgac aatttccccc atgaatcctg ttggttctaa gatcactgaa ttaataagtg
     1801 tcacaaatcc tgctaacatc ataaatggca taaatgtagc aaacgcatct cgcaaagttc
     1861 ttaaatggat ctgattcccc aacttagtcg caactatacc aaatttttca aatacaatgt
     1921 ccttcctgct acttttgccc atattttttc tcctttatgc ttattgtaat cgattacttt
     1981 ttccacccaa ataatacatc acttatcaaa aaaagtaaat acttttaatt aaatatatat
     2041 atctttttta gtataaaaaa ataagctctg aaaaaataat tttcccgagc ttatttttca
     2101 acaaaaacag cttcaaaaac aaagtcttga tataaatgtc tcgataccga atactcgaaa
     2161 ggtttaccag acgatagagc aacaactttg tctatctcaa tcataaattc acctggatct
     2221 atttttaata tttttgcgtc aaattcattc gcagtatctc ctttgatacg tatgtgcgaa
     2281 gtctttattt ccagacccag ttcatttgtg atataagcat atacagattt ctttaaatgt
     2341 tttggttcca agcctggtat gactgctaat ggcatgaatg tttgctcaat cgagtatggt
     2401 tgacaatcaa taaaacggct tcgtacaata ttatatacat agtcttttac actgatactt
     2461 aatttatcag ctactttttt cgatggaagt tcaattgaga aagtatggat ttctgaatct
     2521 acgtgttctt caccaaccac gctaaacaaa ccttcgtttg gaccaagaac tcgtgagtta
     2581 tcaattggtg ttctgacgac ttcagtccca tacccacttc tacttcgcaa aactccttcg
     2641 gaaacaagta agtctacgcc tttctttact gttaacttgc ttgtatgata aataactgcc
     2701 aattcatctc ctgtaggaag tttctcacca attttatact ctccatttac aatttttttc
     2761 tttatatcta agtaaatttc catatacttc gcaatttttt tcaatggtga ccctccaatc
     2821 aaaaacatat cttcatctat actttaacat aaataaaccg ataaaatcca catcaaaatt
     2881 atacacacaa taaaaaagat atatatattt aaacaaaact atttacttta agttgtaagc
     2941 ggtgttatca ttaagtggaa acaggaggta aaaaaattat gacaactatg aaaaagctaa
     3001 cttttccaga tgatttttgg tggggttccg cttggtcagc tgagcaagca gaaggaaaag
     3061 gagaaacagg aaaagcagag actatctggg atcgctggtt taaagaacaa ccttaccggt
     3121 tttatcaacg aatcgattcc tctattacaa ccgatcacat ccatcaatat aaagaagata
     3181 tagaactaat gaaaaaaact gggcataact cctttcgcgt ttctatttct tgggcacgaa
     3241 tgtttccaaa tggcggtacc ggaaaagtaa atcccaaagc catttctttc tatcgaaaat
     3301 tattttccga aatgaataat aacggaatca aagtatttgc caacttatat cattttgaca
     3361 tgcctgctac tcttcaagat aaaggaggat gggaatcacg tgaagtagtt gatgcttatg
     3421 ttcattacgc tgaaacttgt tttaaagaat ttggtgactt agtttgtcat tggtttacct
     3481 tcaatgaacc acttgggcct attttaggta cttatcttga agattttcat tatccaaacc
     3541 tcattgactt taaacgcgga gcgcaagcag cattcttcac tatacttgca cacgcaaaag
     3601 caatacaagt atttaaagaa ctagctcttc ctagtaaaat aggcgttatt ctaaatttga
     3661 gtcctacttt tcctcgtagt caaaatcctg ctgatgtgaa agcagcagaa atcgctgatt
     3721 tattttatac gcggagcttt ttagatccta tggttaaagg gaatttttct aaaaagctta
     3781 tcctgttatt acaagaatat aatcagcttc ctgaagatta tacgaaagaa gatcttgtat
     3841 gtatctacga aaataccgct caaattcttg gactaaacta ttacgagcca cgtcgcgtca
     3901 aatctcgttc ttctatggtg gatacatcta gtccattttt accagagtgg tttttcgaac
     3961 cacatatcat gcctggacga cgaatgaacg aataccgggg atgggaaatt tacgaaaaag
     4021 gaatttatga tttgtgcatg gatatcaaga accattatgg aaacatcgaa gcatttatct
     4081 cggagaacgg aatgggcgtt gcggatgaag aacgtttcat ggatgataca gggcaaataa
     4141 ttgatgatta tcggattgag tatatcaaag atcatcttgc ttacttatgg aaggcaataa
     4201 atgaaggatg taacattaag gggtatcatc tttggacatt tattgattgc tggtcatgga
     4261 tcaacgccta taaaaaccga tatgggctgg tttcactcga ccttactact caaaaaagaa
     4321 caattaaaaa aagtggcgaa ttttacaagc aattaagtga tacaaatgga tttagatacg
     4381 atgtagactt gttagttgaa taatattcta aaattccact tgaagtgaac acattagaaa
     4441 acaagaataa tgagaaatac caataacaaa tactttttct tcatacttca tttatacttg
     4501 tcagtataag agactgtgac aaatgtagtg atcctcagaa gttaggtctg gaaatctaac
     4561 ttgagaagat ttttttaaac tacgtccagt cttttttatt tgtttaaaat tgaagttgac
     4621 aagtcttcat taaaaattta gaatattcac aagtttgacg acaattatga aaatttatgt
     4681 cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac
     4741 acgttaccta ccgtttttgg ttatatcggt attggacttg catttggtat cgttgggaaa
     4801 gctgccgggt ttcatccgct ggtcgtcacg ttgatgtccc tgctgatcta tgctggttct
     4861 gcccaattta tcacggtcag catgcttgct agccatagtc cactgctctc catcgttctc
     4921 tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc
     4981 aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc
     5041 gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat
     5101 acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta
     5161 gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt
     5221 atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg
     5281 atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat cccaagtaac
     5341 ttagtgattt tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc
     5401 ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag
     5461 gatcctacca tttgtcttac tgaaaaaatt cgatttgccg caaccgttgc ttgagtattt
     5521 aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa
     5581 tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt
     5641 agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gtaggaattc tttccctttc
     5701 gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct
     5761 cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac
     5821 ggaccactaa caacgacttt tcctaatata tgtcctgttt ctacgtcttt ggttgcttgt
     5881 ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc
     5941 ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag
     6001 ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa
     6061 acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg
     6121 acaaggtgtt tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct
     6181 acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga
     6241 tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact
     6301 tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc agctccgttg
     6361 atcactaaga tttttttccc gcggttggca ttttcttctg gagtcagacc aaatttttca
     6421 aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcggcttc ttcatctgaa
     6481 agattttttg gtgcaagagc gacaatacgt tcgtcaacca gttggtattc ttgattgctt
     6541 cctgctcttg tcgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact
     6601 tcatcaccta cagcaacaac tttcccgact ccatcaaacc ctaagatacg taaagcattt
     6661 cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact
     6721 tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt
     6781 tttggatcat cgatcgacaa cccttcataa aaaccgacta ccttcattaa ttccttttct
     6841 gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtt ttcatttcaa
     6901 tatatatagt atgctatttg ttccactttt attcatcata attattctct tacttaaagt
     6961 aaactatttg cacttttttg gttaaaaaag aggttgcgac aagacttttg tcacaacctc
     7021 ttttaaacgg tgttcaatca actgaccctc ggtattcgct caaaaaacg
//
LOCUS       DASJON010000147         7068 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJON010000147 DASJON010000000
VERSION     DASJON010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.149"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            126..1073
                     /locus_tag="U3989_002205"
     CDS             126..1073
                     /locus_tag="U3989_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN1715639.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1141..1434)
                     /locus_tag="U3989_002206"
     CDS             complement(1141..1434)
                     /locus_tag="U3989_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN1715640.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(1462..2127)
                     /locus_tag="U3989_002207"
     CDS             complement(1462..2127)
                     /locus_tag="U3989_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN1715641.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(2267..2899)
                     /locus_tag="U3989_002208"
     CDS             complement(2267..2899)
                     /locus_tag="U3989_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1715642.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(2906..3973)
                     /locus_tag="U3989_002209"
     CDS             complement(2906..3973)
                     /locus_tag="U3989_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1715643.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(3970..4701)
                     /locus_tag="U3989_002210"
     CDS             complement(3970..4701)
                     /locus_tag="U3989_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN1715644.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(4870..5349)
                     /gene="greA"
                     /locus_tag="U3989_002211"
     CDS             complement(4870..5349)
                     /gene="greA"
                     /locus_tag="U3989_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN1715645.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(5485..6660)
                     /gene="mltG"
                     /locus_tag="U3989_002212"
     CDS             complement(5485..6660)
                     /gene="mltG"
                     /locus_tag="U3989_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN1715646.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            6864..>7068
                     /locus_tag="U3989_002213"
     CDS             6864..>7068
                     /locus_tag="U3989_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN1715647.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEK"
BASE COUNT         2096 a         1455 c         1105 g         2412 t
ORIGIN      
        1 gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag
       61 caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat
      121 taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag
      181 aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac
      241 aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt
      301 ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt
      361 tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg
      421 agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac
      481 agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa
      541 tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag
      601 acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt
      661 tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac
      721 ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa
      781 aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg
      841 ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc
      901 cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt
      961 tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact
     1021 ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga
     1081 aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt
     1141 ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa
     1201 atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat
     1261 ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc
     1321 atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag
     1381 atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac
     1441 aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc
     1501 tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga
     1561 agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct
     1621 gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc
     1681 taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg
     1741 cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc
     1801 aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc
     1861 ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac
     1921 atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac
     1981 aacagcatga gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc
     2041 cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag
     2101 acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt
     2161 ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa
     2221 taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac
     2281 catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata
     2341 tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct
     2401 gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt
     2461 catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg
     2521 gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg
     2581 tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt
     2641 catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt
     2701 ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc
     2761 gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa
     2821 tgtcttcttg aatagataaa taaaaagaga cgcctaagcg caccatttca tggtcatcta
     2881 ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta
     2941 cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc
     3001 gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg
     3061 ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta
     3121 attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca
     3181 agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct
     3241 ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt
     3301 ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga
     3361 tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg
     3421 cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat
     3481 catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc
     3541 catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt
     3601 tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac
     3661 ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct
     3721 tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta
     3781 atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg
     3841 cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg
     3901 aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata
     3961 tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag taataatctt
     4021 caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa
     4081 agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat
     4141 ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat
     4201 attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac
     4261 ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa
     4321 ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat
     4381 ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt
     4441 cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa
     4501 gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg
     4561 atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc
     4621 agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa
     4681 aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca tttcatcata
     4741 cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa
     4801 aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt
     4861 tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt
     4921 agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc
     4981 attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc
     5041 ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc
     5101 atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat
     5161 tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag
     5221 atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt
     5281 tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt
     5341 ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag
     5401 gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat
     5461 gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat
     5521 atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata
     5581 ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc
     5641 aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt
     5701 atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga
     5761 ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc
     5821 attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt
     5881 caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac
     5941 catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa
     6001 taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc
     6061 tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc
     6121 tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc
     6181 ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg
     6241 caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg
     6301 aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat
     6361 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac
     6421 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
     6481 cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat
     6541 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
     6601 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
     6661 aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg
     6721 gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata
     6781 agattaaaat aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa
     6841 gaaaggagac taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt
     6901 catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc
     6961 ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc
     7021 aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaa
//
LOCUS       DASJON010000148         7037 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJON010000148 DASJON010000000
VERSION     DASJON010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.150"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..379
                     /locus_tag="U3989_002214"
     CDS             <1..379
                     /locus_tag="U3989_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEN1715648.1"
                     /translation="NGNQWQVEIGILKMNVSEDELTPVAPQKEPTQRVIHAVRSESSS
                     HVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQVTIVHGKGTGALRKGITDYLKNHR
                     SVKSFEFAPANQGGNGATIVKFK"
     gene            466..780
                     /gene="trxA"
                     /locus_tag="U3989_002215"
     CDS             466..780
                     /gene="trxA"
                     /locus_tag="U3989_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN1715649.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            962..2740
                     /gene="uvrC"
                     /locus_tag="U3989_002216"
     CDS             962..2740
                     /gene="uvrC"
                     /locus_tag="U3989_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN1715650.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            2885..3784
                     /locus_tag="U3989_002217"
     CDS             2885..3784
                     /locus_tag="U3989_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715651.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            3777..5039
                     /locus_tag="U3989_002218"
     CDS             3777..5039
                     /locus_tag="U3989_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1715652.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            5200..>7037
                     /locus_tag="U3989_002219"
     CDS             5200..>7037
                     /locus_tag="U3989_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEN1715653.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKG"
BASE COUNT         2425 a         1162 c         1470 g         1980 t
ORIGIN      
        1 aaacgggaat cagtggcaag tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga
       61 gctgacgcct gttgctcctc aaaaagagcc gacacaacga gttatccatg ctgtacgatc
      121 tgaatctagt agtcacgtgc cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc
      181 gctaagtgag gtagatcaat atttggattc tgccattcta gctggttatc ctcaagtgac
      241 tattgtccac ggaaaaggaa caggcgcact tcgcaaggga attactgatt atcttaaaaa
      301 ccatcggagc gtcaaaagct ttgagtttgc accggcaaac caaggcggaa atggtgcgac
      361 aattgttaaa tttaagtaaa gcttactaaa ggtaagcaga tggttcgcta tccaagtata
      421 gaaatgctat actagtcata gattttaaaa atggaggtct ttgatatgtc acaagtaatt
      481 acagataaga catttaatga agaaacagat aaaggacttg tattgattga tttttgggca
      541 acttggtgtg gtccatgtcg tatgcaagca ccgattttgg atcagttgga acaagaatat
      601 gatgaagaag aattcagaat tgctaaaatg gatgtggatg aaaatccaga aactcctcaa
      661 caattcggta tcatgagcat tccaacatta atgttgaaaa aagatggtca agtagtagaa
      721 aaagctgttg gtgttcattc aaaagaacaa ctacgtcaaa tgatcgatca atatttataa
      781 aaacttacag caaaaataag ctgagggtgg gactgaatcc agttccgtcc tcggtttttt
      841 tgcggttgag ttaaggttat tcaaattaat attttttgag tataattacg ttgtcaggct
      901 ggattgtttt tttgacagac atgctataat aactttaatt gaatatgaag gggagtacca
      961 gatgaacgaa cgaatcaaaa acaaattagc tttacttcct gatcagcccg gctgttactt
     1021 gatgaaagat aaaaatggaa cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg
     1081 tgtccgctca tattttactg gtagccataa tacgaaaacg gaacggcttg ttagtgaaat
     1141 cgaagacttt gaatacatcg ttacagaatc gaatatcgaa gcattgctct tagagatcaa
     1201 tctgataaag aaaaatgatc ctaaatacaa cattatgtta aaggatgata aaacctatcc
     1261 tttccttaag ataacgaatg aaaaatatcc ccgtttggtg attactcgta aggtactaaa
     1321 agacaaagca ctgtattttg gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa
     1381 gatcctcgat cgattatttc ctctgagaaa atgcaaacct agtcagacga aggagccttg
     1441 tctttactat catttgggac aatgcctgtg tccttattat ttcgacgtag atccgaaagt
     1501 ctatacgaaa atcgttgatg aagtgaaaaa ctttttaaac ggaggacacg aaaaaatcga
     1561 gtcagagatc catcagaaaa tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc
     1621 agaataccga gaccagatcc gtgccattga gacaatcatg acttgccaaa aaatgacaaa
     1681 tactgattta ctggatcgag atgtatttgg ttatgcgatt gataaaggat ggatgtgtgt
     1741 ccaagtgttt ttcgtaagac aaggaaagct aattgaacga gatgtttcaa tgtttccttt
     1801 ctataacgaa gcagaagaag atttcttgac ttacctcggt cagttttatc aggaaaatga
     1861 gcatttcatt cctaaagaag tcttgattcc tgatacgatc gacaagccca gtgtagaagc
     1921 tttgttagct acgaaagtgc tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt
     1981 ggcgagtaag aatgcctcag ttgctttgaa tgaacggttt gatctgattg ccagaaaaca
     2041 ggaacggacg atcggagcag ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat
     2101 acgtatcgag tcttttgata actctaatat catggggacg aatcctgtgg ctgcgatggt
     2161 cgtattcatt gatggaaaac cggcaagaaa agaatatcgg aaatataaaa taaaaacagt
     2221 caacggaccg gatgattatg catcaatgag agaagtcatc tatcgcagat attcgcgagt
     2281 gattcgcgaa gggttgcctt tacctgattt gatcttgatc gatggaggaa aaggacaagt
     2341 agatgtggca aaagatgtct tagaaaatca attaggaatc gatattccta ttgctggcat
     2401 ggtaaaaaac gataaacata aaacaaatga gttgctattc ggtacagatt tggctgtagt
     2461 accgcttgag cgaaattcac cagagttttt cttactccag cgaatccaag atgaagtcca
     2521 tcgattcgca atcactttcc atcggcagct acgcagtaaa aacagttttg cttcccgatt
     2581 agatgaaatt gaagggttag gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc
     2641 gttgaaaaat atcacagcag ccacgacaga agaattacaa gaaatcggac tgccgaaaaa
     2701 tgtcgcacaa aacgtatttg ataaattaca tcaggaatga ggaaagggca ctttacaaag
     2761 aaagtaaagt gtcctatcat tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa
     2821 ataataaatc gcttacgtct ggtgtctaag gatatatgtg atttcttgaa gaaggagtga
     2881 agaaatgtta gaagtcaagg atcttgttaa aacttttggg aactacacag ctgtcgatca
     2941 tgtctctttc caaatccccg atggaaaaat catggggcta attggccaaa acggggctgg
     3001 gaagacaacg acttttcgtt tgatcttgga ttttttgaca caagaccaag gagaagtgtt
     3061 atggaatggc catcatcttg gggagaaaga ttatgatgtc atcggttatc ttccggagga
     3121 acggggactt tatccgaaag taacgattca agaccaactg ctttattttg cagaacttag
     3181 agggaaatca agaaaagaga ttgaaccaaa aatcgacttt tggatggaaa aatttcaggt
     3241 aaaagggaaa aagacggata aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca
     3301 gctgatcgcc acgttaatcc atgaacctaa attgatcatt ttggatgagc cattcagcgg
     3361 gttagatccg gtaaatgctg aattgctaaa agatggaata ttggaattga aaaaacatgg
     3421 ttcatgtgtc attttttcta gccacaatat ggataatgta gaaaaaatct gcgatcattt
     3481 gatcatgttg cgaaacggaa aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc
     3541 atttggccgt acgaagttat tcttggaatc tggtttaagc caacaggaag tagaagaaat
     3601 cgaaggcgtg aaaaaagtga tccttcatga agacaatacg ttagagatta ccttagaaga
     3661 tcctgaagct gggaaagaaa tctttactcg tgcgactcag ttcggctaca ttcctatgtt
     3721 taaccagcaa ccgccaactt tggaagagat tttcaaattg aaagcaggtg aaatcgatgc
     3781 gtaagttttg gattattacc aaagatgtct atttaaaaaa tgttaagtcg atttctttct
     3841 tgatcatgat attagtccct tttatcatgc tgggtatcat ttatgtagca ggaaactttg
     3901 ctcaacaaaa tagtgatgtc aataaaatcg gcgtattggc tgataatgag caaatcagtc
     3961 agcaaatgag ccaagtcaaa acagatgatt ttgagttcaa agcagttgat tctgaagaaa
     4021 aagcacaaaa agagctttcg gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga
     4081 atgataagat aaatgggatg ctttatagcg aaaattcatt aggacaaagt acacaactac
     4141 tgatacaaca acaactgact ggcttgcaat cgatgcttcg agcgagtcaa ttaggcattt
     4201 caccagaaga agtagctagc ttgagccaac aagcaagttt gactcgacaa aaagtcaatt
     4261 ttgattcaaa aggaaaaatg acagtcggag aagataactc gacagtccaa tacgtcgtaa
     4321 gttatgtagc aacgattgtc ttgttcatta ttattttgac gtatgcgcag atcattgcac
     4381 aagagatcgc ttctgaaaaa ggcacgcgga tcatggaagt gatcctgtcc agtacgactg
     4441 cacaaaagca tttttacgga aaattaacag gtgttttact tgttgcatta acgcaaatgg
     4501 cattatacgg tattattttt ggtataggat acagtcaatt caaagatatg gaaatggtca
     4561 aaaatatatt agacggtatc tcattggata acttgattgg accattcttg tggtattctc
     4621 ttctattcat gatctttggc attttgattt tttctgtttt agcagcttta tgtggctctt
     4681 tggtcaacaa agcagaagat acagcaaaag caattttgcc agtgacttat ctatctctag
     4741 gcggatacat gcttggattg atactaggag cttcagatcc aaacaatatc gttattcgga
     4801 tcacttcgta tattccattc ttatcttcat atattatgcc gattcgctta gcaaatgaga
     4861 cagtcggtat gggtggagca ttgatttcct tgttggtact tgtagccgta acattcttat
     4921 tgatgatctt gtctgcaaac atgtacaaat cgaatgtact cgtttatagc gaaggcggat
     4981 tatggagttc attgaaacaa tcgatttcaa ttatgaggaa tgaacgaaaa aaagcataaa
     5041 acaatcctaa aatacagaag gaagctggac atctattgtt cccagcttct ttttgtatag
     5101 acctatttta tttattttac tatattggat atatattcat tgaaaatagt gtataacaac
     5161 tttataatat tgagtatacc aatttttgga ggcgttttaa tggaccgtta tttagattta
     5221 ctttcagaaa aattcccctc aacggaggat gtcatcacgg aaatcatcaa tctcgaagca
     5281 attttgcagc ttccaaaagg aacagagtta tttttgagtg atatacatgg cgaatttcct
     5341 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
     5401 ttttctgaac aaatgacgga aacggatatc agccaattca ctatttttac ggcttatcca
     5461 gaatatgcat taacgactga ttggtacaaa gaacaagata aatcgcagct gatccatcat
     5521 ttgatcgatc ttttacggtt tacgacagtc aaatataccc ggtcaaaagt tcgaaaagga
     5581 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga ccgtatcaat
     5641 ggaaaaaaag actatgtaaa aaaaatcatt gaacaacttg ttttgatgaa ggaagaagaa
     5701 aaatttttaa cgaaacttgc acagacgatc caaaaatctg tcatcgatca tttgcatatc
     5761 gttggagata tttttgaccg aggaactcaa gcagccaaag tgatggatcg aatcatggat
     5821 ttttcttctg tagatatcca atggggaaat catgatattt tgtggattgg tgcgtattgc
     5881 ggctctgagg catgtttact gacactttta cggattgcag cacgctataa ttatttattt
     5941 gaattagaga aagaatatgg gttgaattta cgtccattgt tttcttttgc tcatcaaact
     6001 tatcagaaga atccagtatt tgcgcctaaa aacagtaaaa atctatccga aagagagcaa
     6061 gaagaattag aaaaaatcca tcaggcactt tctatccttc aattcaaatc agaacatcag
     6121 ttattggatc gtcgacctga atttaaaatg gacgatcgta aattgctgga aaaaatcgac
     6181 tatgaagcat caacgattga tttagaaggt cagctctatg agttgaaagg aacttgtttt
     6241 caaacgatcc agccagatga tccaaaaaaa ttcttggaag aggaacaaaa agtgattgat
     6301 agtttgatgt attcattcca gcactctttg agaatgcaaa aacatatgac gtttttgatc
     6361 gaaaaagggg gaatgtatct cacgaacaat cagcatgtgc tgtttcatgg ctgtatacct
     6421 gtagacgaga agggacagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
     6481 ctgctgggct tttttgaaaa acagataact gaaagcgcaa aagatctgac tgcaaaagaa
     6541 gatttagcaa ccgatttgat ttggtatgcg tggagtgggc cattttcgcc tttattcggc
     6601 aagagtaaaa tggcgacttt tgaacgttat tttctcacag agcctcatac gcacgtcgag
     6661 aaaagcaatc cttattatca cttgcgagat gaagagtggt tcagtgaaaa aattttagca
     6721 gaattcgaca caaaagaaga agggtcagca atcattaatg ggcacacgcc agtaaaagta
     6781 aaaaaagggg agtcaccgat caaagcaaat ggaaaagtat ttgtcattga cggaggttta
     6841 tccaaagctt atcaaaaaac gacaggaatt gccggttatt cactattatg caattcttat
     6901 ggtttccaaa tcgtgacaca ttatccgttt ttgcctattg aacaacaatt tgcaaaaaaa
     6961 tccgaccaaa caaatgtgaa aaaagtaatc gaacagcaac taccacgcaa actcaatcgt
     7021 gatacaacaa aaggtat
//
LOCUS       DASJON010000149         6884 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJON010000149 DASJON010000000
VERSION     DASJON010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.151"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..1071
                     /locus_tag="U3989_002220"
                     /pseudo
     CDS             1..1071
                     /locus_tag="U3989_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase"
     gene            1439..2362
                     /gene="truB"
                     /locus_tag="U3989_002221"
     CDS             1439..2362
                     /gene="truB"
                     /locus_tag="U3989_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN1715654.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            2366..3307
                     /gene="ribF"
                     /locus_tag="U3989_002222"
     CDS             2366..3307
                     /gene="ribF"
                     /locus_tag="U3989_002222"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN1715655.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            3659..4444
                     /locus_tag="U3989_002223"
     CDS             3659..4444
                     /locus_tag="U3989_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1715656.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            4457..5308
                     /locus_tag="U3989_002224"
     CDS             4457..5308
                     /locus_tag="U3989_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN1715657.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(5432..5884)
                     /locus_tag="U3989_002225"
     CDS             complement(5432..5884)
                     /locus_tag="U3989_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715658.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(6349..6717)
                     /locus_tag="U3989_002226"
     CDS             complement(6349..6717)
                     /locus_tag="U3989_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN1715659.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         2312 a         1135 c         1421 g         2016 t
ORIGIN      
        1 aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa aatgatgaat ggctttcgcc
       61 atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa gatgaagggt tcttagaagg
      121 gtacgttctg actggacggc atggtttttt gcaagttacg aatcattctt gcgagtggtt
      181 gactctatgt tgactcagca cttcaaatgg atgcggaaat cacatgacct ttcttggcga
      241 aatgattatc cttcgctaaa tctcattgca agctcaacag tcttccagca agatcacaat
      301 ggatatagcc accaagatcc aggaatattg acacatttgg cagaaaagaa ggcggagttc
      361 atccgtgaat atctgcctgc ggatgcaaat acattgctcg cagtcatgga taaagcattc
      421 cgctcgtcag aaaaaatcaa tttgatcatc tcttccaaac atccgcgtgc tcaattttac
      481 tcagcagaag aagcagccgt cttagtgaat gaaggcttga aaatcattga ttgggcttcg
      541 acagcaaaag aggaggaacc tgaattggtc attgctgctg caggaacgga atctaactta
      601 gaagcattag cagctgtaac tttgctgctg gaagaattcc caaaactaaa aattcggttc
      661 atcaatgtcg tagatttatt gaaattacgt catccaagtc aagatccgcg tgggttgagc
      721 gatgaagaat ttgatcaata ttttacaaaa gacaaaccga tcttatttgc tttccacggt
      781 tatgaaacat tggtccgcac gattttcttt gatcgtcata accatcattt aatgattcat
      841 ggctacaaag aaaatggaga tatcacgaca ccatttgaca tgcgtgtagt aaatgaactt
      901 gatcgttatc atttggcaaa agacgcagct ttaaaaatca aaggaagtca agcagaagat
      961 tttgcgaaaa aaatggatca gaaattacaa gaacatcaaa actatattcg tgaaaatggt
     1021 attgatctgc cagaagtatt ggattggaaa tggaaaaatc ttgaccaata aaatgataaa
     1081 atcgatcccg caaaatagaa ttttttacat tgattaagaa actgaaaaaa taaataaaaa
     1141 aaacggaaaa aagactggaa gaatggccag tctttttttt cgtttttggg aagtgaaaac
     1201 caaactattt acctgtaagc cacccataaa tccaatcaaa tggtgcaaag aaaaaatctt
     1261 ccattctatt ccctccttta aaaaagcata acatgatttc tatttacgtg agcatgaatt
     1321 ttgttacgtt tttattgccg aaaaataaaa accgttcatt atgatgtcaa attcgtcagt
     1381 aattgatgca atgtgttata ataattcagt taaatcaaaa atgaaatgga gaaaagacat
     1441 ggacgggtta ttacctttat ggaaagaacg cggaatgaca agtcatgatt gcgtattcaa
     1501 attaagaaaa atcttacata cgaaaaaaat cggacatggg ggtacgcttg acccagacgt
     1561 agacggtgtg ttgcccattt gtattggaaa agccacaaaa gttatcgaat atttgacaga
     1621 ttctgggaaa atctatgtag gcgagataac tctaggtttc agtacaacga cggaagatcg
     1681 atccggagaa cgggtagaaa caaaaccagt agatatgtct ttatctgaaa aaaaaataga
     1741 tgaagtgatg gcttcattta ttggtgagat cacccagata ccaccaatgt actcagcggt
     1801 aaaagtcaac ggtaagcggt tatatgaata tgcccgcaat aatgaaacag tggagcgtcc
     1861 tgttagaaag gctcagattt accgttttat ccggacaagt gaattgacaa gagacgaagt
     1921 gacaggaacc atttcttggc gcttcgaagt agaatgcggg aaaggaacct atgtccgcac
     1981 attagctgta gatacaggca agaaacttgg ttaccctgcg cacatgtctg atctgacccg
     2041 aacagcaagt gccggtatgt cccagaagga tgctctgaca ttagaagaag tagctcaagc
     2101 tgtgaaaaat gggacaatcg atgattgttt gctgccaata gaaaaaggag tggaacggtt
     2161 caaacgagta gatattactg atgaagtatg gcagaaagta aaaaacggta tgagacttga
     2221 ttatactgta tttgcgctcc cagaaatgcc gtcagaagaa attgctttgt tttataaagg
     2281 aaaagttgtc agtatttatc agccaaaccc gaaagaaaaa aacaaattaa aacctagtaa
     2341 agtattgaga aatgaggaat aacgaatgaa tatcatcaag atcagacatc cttaccaatc
     2401 agaacaaatt ccagaagacg atgtcgtttt agtgctggga tttttcgacg gcgtgcatat
     2461 cggacaccag aaagtcataa agacaggtat ggaaattgca caaaaagaag gattgaaatt
     2521 agcattgatg actttcaatc agcatccatc tattgtcttt aaaaagatcg atcctagcag
     2581 cgtgaaatat ttgactagcc ttaagcaaaa agaagaaaaa atggccgctt tgggtataga
     2641 ttatttatat gaagttgatt ttacttcttc ttttgctcat ttagcaccgc aggaatttgt
     2701 tgatcaatac atcgtagggt tacatgcaaa atatgctgtt tccggttttg attatacgta
     2761 tggaccaaaa gacattgcag atgtcgctca ttttcctgaa tatgccaaag gaagattcaa
     2821 aatcgtcaca gtttctaaag aagaagaaca aggtgaaaaa atcagctcta cccgtattcg
     2881 tggattacta gacagcggac aagtaaaaga agcagcaaaa ctattaggtt gcacatatga
     2941 ggtagaaggg gtagttgtcc atggcgatgc acgaggcaga ctactaggat tccctacagc
     3001 aaatgtcaaa gtaaagagca cggttcattt gcctaaagaa ggtgtttatg tgtcggaaat
     3061 aaaaattggg gaaacatggt atccagccat gggttcaatt ggacataatg atacattcgg
     3121 tgaaggacgg gagctgactg tagagttgta tattttggac tttaatcaag atatctatgg
     3181 tgaacatgtc gcggtacgct ggaatcaatt tttaagagat caagtgaaat tttctagtgt
     3241 agaggaattg attgaacaac tcaaggagga tgaacaagca acagctgaat atttttctca
     3301 gcaataaaac attatgcata aaatctcttg tttatgtata actagcaatt acaataaaat
     3361 gggtgataaa taagcccagg aaaggggctg tgacaaaagt cttgtcacag cctttttttc
     3421 caaataaatg gtttttcaga agtagcttct tcggaaataa gccgaaattt cttgaagctg
     3481 accacttctg tcacaacctc tttctttata gaaataaaag gacactagca attcatgaaa
     3541 acgattgcta gagcgtatca tttgctttag actatatatc ttggtgtaca ctcactttaa
     3601 ggcggaaata aaagttatcc gtaataacag gtttaggtat gaaaaaggag tgggtactat
     3661 gtacaaggaa ttagaaaaca aagtcgcagt agtaactggt ggctcaaagg ggattggaac
     3721 agcgattgct caacgattcg ggaaagaaaa aatgaatgta gttgtcaact ataactcgga
     3781 taaagaagga gcagaaaaag cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc
     3841 tgtacaagca gatgtcagtt ctgaagaagg aatacagcgt ttacttgata cggctttaga
     3901 aaattttggg acactggacg tttgggttaa taatgctggg atggaaaatc agcatccaac
     3961 ccatgaactt agccttgaag attgggaaag agtgttaaaa gtcaatttga ctggcgtttt
     4021 tatcggaacg aaagcggctt taaattactt tacagagaat aataaaaaag gaaatatcat
     4081 caatatgtct tctgttcatg aacagatccc atggccaacg tttgctcatt acgctgcttc
     4141 aaaaggcggg gtaaaattat ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat
     4201 cagaatcaat agtataggcc ctggtgctat ccgtacacct attaatgcgg caaaatttga
     4261 tgatcctgaa caaaaagaac tcttggaaag tatgatccca ctaggacgcg taggtgaacc
     4321 aaaagaaata gcggcgacag ctgcatggtt ggcttcagac gaatccagtt atgttacagg
     4381 tattacacta tttgcagatg gtgggatgac tttatatcct tctttccaag gtggaaaagg
     4441 ataagggtga ataaaaatgg gtttattgat tgcattgatc ccggcagttg gctgggggat
     4501 ccagccactg attttgaaaa agattggtgg cagaccaaca aatgagatat taggaactgg
     4561 tattggagcg gtgcttgtcg gcttactcgt tcaattattc gcttctccag ctggtatttc
     4621 agtgactaca tttttgatta gtttactttc tggtgcattt tgggttattg gacaaacagg
     4681 acagtataca tctttcaact tgattggtgt ttctaaaacg atgccaattt cgactgcatt
     4741 acagttagtc ggaacctcgt tgattggtgt cttcgctttt ggagaatggt ctggaagtat
     4801 ggggaaaatc attggagcag cagcaatcat tcttcttgta tttggttctg ctttaacggc
     4861 aatcagtgat gatcctggaa aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc
     4921 aacaagtatt ggttattggg tatacagtgc attgcctaaa ctagttgatg caagcggact
     4981 ttccattttc tttccgcaaa tgatcggtgt ctttttaggg gcagtgattt atgtgatttt
     5041 ccgtaaacca accgcatttt ctgaagacaa gagttggaaa gcaacgatcg ttggagccat
     5101 ctttagtgtg tcagcattgg cttatatttt ttcagcaaat gaaaatgggg ttgctactgc
     5161 atatatcatt acacagctaa acgtagttat ctctacatta ggcggtatgt acatcctgca
     5221 tgaaaataag acaccaaaag aaaaaaaatt aacgattatc ggattattgc tgatcgtagt
     5281 aggaagcatg gtcacggtct ttatttgagg ataaagatgg taaaatttca tttattaaaa
     5341 aaatatttgt ttgaaccaaa cagtaagtac ttataaaaaa atagcgctag aatttccaac
     5401 atctctggaa tttctagcgc tttttaattg tttaagatac aagggaaata tctgtctgga
     5461 tcaataattt ttcattgatc cattgactaa agtttacgac ataattgaag ttaccaccga
     5521 ttagtatctc cgcatgattg tctttataaa gatcgtattg taaaagacgg tcttttttat
     5581 ttgcgtaaac atcgttatgg tactggacta attgttctcg gaattgattg attgctgtaa
     5641 aatcacgata ttgaaggttg aaacgcatcg tgattggtgc atttagcatg aatcgctgca
     5701 tttcttttgc tgcttcctct gcttgatctc cgtataggaa ctcaaaaaga ttacgttcag
     5761 aaaatttcca ttgcccgcca acctttgttc cttttagttt tccttcatga agataatttc
     5821 tgatagaacg tgtagtaaca cctaattgtt cggctaattc atttactgag taaaaatctt
     5881 tcaatgttca ccctcctatg tgaatggttc tactgactta gttgatattt gcttaatcac
     5941 gaaatgtaga aagaacgatt ttaattaagg tcattgtaaa aatcaagtca gaaaattcta
     6001 acttttattt ctctaaagaa attcgttctc tcctaaagtg atattatatt taaatatacc
     6061 tgaaaataat gggcagtatt atgaaagatc actgatattt tctcgtattt tttccttatt
     6121 caaaagtttt gcaggaaaat tgctgaaaaa ataattttac taagtagaaa attcaaagcc
     6181 attcaatcag tgattttttt cagcacaaga aagactcctt ataaaaatag cagatacttt
     6241 gtcaattaag tgtttaacca gggatgataa aaaggatagg attgaacaaa acctgtataa
     6301 ggtgatgaat agtttcctcg ccagtaaaag gtttattcgt ataaagcttt atcgccaaaa
     6361 tttatcatga ccttttttat cttcaatatt aaatgaaatc aattgttcaa tcaattccca
     6421 gttgattaca cttttccaag gaactttgat gacgttatcc gtatatacgt agcctgcttc
     6481 ttggatattt ttttcaaggg cacgaatcgc tgctttttcc ggtgcgatag aaaaatgatt
     6541 tttagcaaaa ctaaatgcaa taataaaggt accatggtca gtaaacatag gttggttcca
     6601 ttttacagtt gtgtctagct ggggatacgt ttctgttatc ttagcaaaaa ggtctttgat
     6661 cttttgttga tgttcttttt ctttgatttg attgatgatc ggctgaaatt tttccattct
     6721 aaaacgcctc cattaagtgg tttgatttat tttaccagaa aaacaatagt tatgtataaa
     6781 aaacgaacaa aacacaagta atgttgtaac cgctatattt atatgataag atgtctcaaa
     6841 taataaaata gaaaagagac ataaaaatga ttgacttggc aaag
//
LOCUS       DASJON010000150         6860 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJON010000150 DASJON010000000
VERSION     DASJON010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.152"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..578
                     /locus_tag="U3989_002227"
     CDS             <1..578
                     /locus_tag="U3989_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1715660.1"
                     /translation="SKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAIS
                     DEGIRKLRAAYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTST
                     QDTTSSDSETAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAE
                     RTGVPVETILSLNGMSMENYFLTPGETLKLN"
     gene            complement(633..2003)
                     /locus_tag="U3989_002228"
     CDS             complement(633..2003)
                     /locus_tag="U3989_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1715661.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            2241..2987
                     /locus_tag="U3989_002229"
     CDS             2241..2987
                     /locus_tag="U3989_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1715662.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            2984..3823
                     /locus_tag="U3989_002230"
     CDS             2984..3823
                     /locus_tag="U3989_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1715663.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            3824..4774
                     /locus_tag="U3989_002231"
     CDS             3824..4774
                     /locus_tag="U3989_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1715664.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            4915..5667
                     /locus_tag="U3989_002232"
     CDS             4915..5667
                     /locus_tag="U3989_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715665.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            5686..6837
                     /gene="queG"
                     /locus_tag="U3989_002233"
     CDS             5686..6837
                     /gene="queG"
                     /locus_tag="U3989_002233"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN1715666.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         2362 a         1231 c         1417 g         1850 t
ORIGIN      
        1 attcgaaaaa gatcgaagaa aagacgcctg cattgattga agaataccag acagaaattc
       61 agggaaatca agagggaata gctggtttat cagcagttgc taaccaaaaa gcacgtgagc
      121 ttcaagcaat ctccgacgaa gggatcagaa aattacgtgc agcgtatcag gcagcagaaa
      181 ataaagacgg tgtagactta gatactttga tcaatcaatt atcggctaat tatacgaatc
      241 atgtcgctaa gatttccgat atttatttgc agactagcgc ctctcttcaa gcagagtcaa
      301 caagcacaca agacacaaca tcttctgatt ctgagacagc agagtcggta gaagcttctg
      361 aaatgcaagc tcgaacaaca caagacacaa cagattcctc tgaaactaat accaacagcg
      421 cgacttcaca ggctgcgaca accgtagttt tggaaggaga aggtccaaat caaatagccg
      481 aacgaactgg cgtaccagta gaaacgatct tgtcgctgaa tgggatgagc atggagaatt
      541 atttcctgac tcccggtgag acgctaaaat tgaattgata aaagcaataa aaaagaacga
      601 agcttccact aaggtgaagc ttcgttcttt ttttattttc cggttctttt ttcgcgtaca
      661 aagtaatgga tcgtcatgta aagcaagaaa acgagtccaa taatcaatga gattctcgtt
      721 tcaggattga taaacatgaa aatcaatacg atagctaaaa aagcaagtgt cacataattc
      781 gtataaggag atagtggcat tttaaaagga tgatcatgca ttttatctgc gtgcttcttt
      841 ttgaagcgaa tctcgctgat caaaatcact acccaaggaa ccataccagg caaaatactt
      901 gagctgtata cgaaaacgaa aatattttct gactggttga tgaacattgg tagaagataa
      961 tttaagatca gaccaattaa gattccacct gagattgcta cgacaggata aaaaggcgca
     1021 ccgtgacgag atagtttcag aaatttttta gacatctttc catttatggc aagtgtatag
     1081 accatacgac ttgcgctgaa aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg
     1141 acaaaattaa tcagtcctgc agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa
     1201 ggtgaaccaa tctcactcaa ttgattccac ggatagatac tcacaatgat aaaaatagca
     1261 ccaatataaa aaatcaagat tcgaccgata gtggactgga cagcttcaat gatcgtcttt
     1321 ttaggattct ctgcttcacc agcagtcatt ccgatcagtt cgattccttg ataggaagca
     1381 acaacgatcg ataatgcaaa gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt
     1441 ttccaaagat ttgaaatccc tactgcctga ccatgattac caaatccaaa taagattacc
     1501 ccaagacctg caatgatcat caaaatgatc gttatgactt tgatgatcga aaaccaaaat
     1561 tctaattcgc caaacgcctt gacagaaata agatttgcca gcataagaaa caagacgaca
     1621 atgattccgg gaataattgc cggcaaatca ggccaccaat aattcatata agatcctaca
     1681 gcaatcactt cacttattcc tacgacaata tattgaaaaa tattactcca agcggtcaag
     1741 tagccagcca caggatggat gtactcgctc gcgtaatttg caaaagaacc agtcgaagga
     1801 tcaacgtaaa gcatttcgcc taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc
     1861 gcataggctc ccatcactga gggccctgtc caacgtatgg ttgaggaggc acccataaac
     1921 agaccgacac cgatcgttcc acctagtgcg atcatttgga tatgacgggc actaagactt
     1981 cgagttaact tttcttcttc catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa
     2041 ataatcaaac gtttcaacac atgtttttca ttattaggtt agccttattg aactcttgat
     2101 gtgattctac agaaaatttt cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca
     2161 ttctacttga aaatgctttc cattttagtt aaactatatt taaataaaaa gttaggtaaa
     2221 cctaaaaagg agtgtaagat ttggaacgta cggccattgt aattcaagac ctttcagccg
     2281 cttatcaagg gaaaactgtt ttgaaaacta tcaacttgac gataaatcca caaaaaatta
     2341 ccggtattat cggaccaaac ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt
     2401 tgataccagc tactactggt aaagtcacct tttctggaca gtcggtaaac tcagtccgta
     2461 aaaaaatagc ctacgtggaa cagcgaagtg aattggatct ttcctttcca attgatgtat
     2521 taggcgttgt acttttagga acatatccat ctttacgaat tggacaaaga cctgggaaaa
     2581 aagaaaaaga acgtgcaaga caagctttga aaaaagtagg gatggaagaa tatgccaaaa
     2641 gacagatcag cgaactatcg ggtggacagc ttcagagagt ttttattgca agagctctag
     2701 cccaaggagc agaatggatc tttttagatg aaccattcgt agggattgat gcgttaagtg
     2761 aacgaaagat ctttgacatc ttgcaggaat tgaagaattc aggaaaaacg attttgatcg
     2821 tccatcatga tcttcataaa gtagaagaat atttcgatga ggttattctt ttaaataaac
     2881 agctgatcgc ttccggtcca gtacaagaag tctttacatc gaagaacctt caattggcct
     2941 atggtgagat catcagacat ttagtaaaag gaggagagaa aaaatgatcc aatcctttat
     3001 tgatggatta atgaattatc aattcctgca atacgctttg gtcacttcca tgcttgtagg
     3061 acttgcttct ggagtcatcg gttcttttat tattttacga gggatgtctt tgatgggaga
     3121 cgctatctct cacgcagttc ttccaggtgt agccatttct tatatgtttg gttttagtta
     3181 catctttgga gccactgctt ttgggatgct aacagcagca gtgattggtt tcgtgactca
     3241 gcggagccga ttgaaaaatg atacagcgat tggaattgtc tttagctcct tttttgcatt
     3301 agggattatc ttgatttcat atgcccagag tgcgacagat ttgtatcata ttttatttgg
     3361 gaatgtctta gctgttaggg aaagcgattt gttgctgaca gcactcgtta gtggaatcgt
     3421 cttgatattt gtctttttct tttataaaga attgaagatc acatcattcg atccgacaat
     3481 ggcaaaagct tatgggctga acacatcgct gatccattat cttttgatgt tctttttgac
     3541 attagtggct gtagtcagtt tacagacagt aggaacaatc ttggtgattg ccatgttgat
     3601 cacaccagcc gccacggctt acttgctaac gaaccattta ctgaaaatga tcattacagc
     3661 tgcaggaatc ggtatgctaa gtgcagttgt cggtgtgttt ttcagttata gttacaattg
     3721 gccatcagga gctacgatcg tgttagcatg taccgcattt tttatccttg cttttttatt
     3781 ttctccaaca aaaggaattt tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc
     3841 actcttttta atcttggcag ttagtttctt ggtattagcc ggttgcggaa agcaggcttc
     3901 tgaccaagcg gatgaaggaa gtaaagaaaa gttgtcagtc gtggctacca attcgatctt
     3961 ggcggacatg gcaaaagagg tcggaacaga tcaaatagat atccacagta tcgtaccagt
     4021 cggaacagat ccgcatgaat atgaagtact accagaagac atcaaaaagg caagcgatgc
     4081 agatgttatt ttatacaacg gtttgaatct tgaaacaggt aacagctggt tcgataactt
     4141 gatggaaacg gctaaaaaag aagaaggaaa agattatttt gcagttagca aaaatgtaga
     4201 acctctttat ttaactagcg gtgaagaaca tacaaaagca gatccccatg cttggctaga
     4261 cctatctaac ggaataaaat atgtggagga aatcgcacgt atattctctg aaaaagatgc
     4321 agaaaatgcg acactctata aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt
     4381 agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat tattagtaac
     4441 aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag cttatatatg
     4501 ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat
     4561 aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa gaagcatgga
     4621 acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg attccatagc
     4681 aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata ttgaaacgat
     4741 ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag cttgaggagc
     4801 agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc gtcgtacaga
     4861 tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt ggctatgaat
     4921 tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat cagccagcag
     4981 gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc taaattattc
     5041 atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg caaatggttg
     5101 tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt gttcctttct
     5161 ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac cgtcaaagca
     5221 tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc
     5281 tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta taatcgtcta
     5341 gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca gcaaaaaaat
     5401 ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta
     5461 aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa tgcattagat
     5521 aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg
     5581 atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa aaaagaaaaa
     5641 ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac attaaaagaa
     5701 gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac cacggcggaa
     5761 ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca tacttctggc
     5821 tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga tcagccgaaa
     5881 tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt gcctagggat
     5941 gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga tatactcaga
     6001 gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa
     6061 gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt cgctgtagca
     6121 cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga agagttcggt
     6181 tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga taaaccagga
     6241 gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa agcattgctt
     6301 ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac aaaagggatg
     6361 atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga tatctgccag
     6421 ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat ggaaccaaaa
     6481 gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa agacttcaaa
     6541 cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca gagaaacgca
     6601 ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc agaatgttta
     6661 acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg tctaggaaca
     6721 aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga aacagaagaa
     6781 gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt aaaataaaaa
     6841 aatagttaac attattcaaa
//
LOCUS       DASJON010000151         6853 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJON010000151 DASJON010000000
VERSION     DASJON010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.153"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..1996
                     /locus_tag="U3989_002234"
     CDS             8..1996
                     /locus_tag="U3989_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN1715667.1"
                     /translation="MQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAA
                     FFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRF
                     WYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVLLIVGGLI
                     LLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNL
                     FKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEIN
                     QTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGG
                     GTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYR
                     RKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQ
                     LSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKI
                     DKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNN
                     FGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQE
                     KNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2056..2772)
                     /locus_tag="U3989_002235"
     CDS             complement(2056..2772)
                     /locus_tag="U3989_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN1715668.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2999..3541)
                     /locus_tag="U3989_002236"
     CDS             complement(2999..3541)
                     /locus_tag="U3989_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1715669.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3546..3773)
                     /locus_tag="U3989_002237"
     CDS             complement(3546..3773)
                     /locus_tag="U3989_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1715670.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3852..5732)
                     /locus_tag="U3989_002238"
     CDS             complement(3852..5732)
                     /locus_tag="U3989_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1715671.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5921..6535)
                     /locus_tag="U3989_002239"
     CDS             complement(5921..6535)
                     /locus_tag="U3989_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715672.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6677..>6853
                     /locus_tag="U3989_002240"
     CDS             6677..>6853
                     /locus_tag="U3989_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1715673.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         2067 a         1386 c         1136 g         2264 t
ORIGIN      
        1 attttatata caaaaaatcc gtttcattca tcgtttcatc gaatacttcg cttttgattc
       61 gattgaacaa ttaactcagc gttggaaaaa aagaattcat tggctttttg ttcataaaag
      121 ttatttttta gttgcagcat ttttttactg ctttaccttc gtgcagattt tcgttttaga
      181 accaaaagaa ggttggaaag aaactatcca ggttgcctta aaaatattta ctcagcgaca
      241 agcacccatg atcctgacaa tcatcattat tatgggattt ttcttcttac tattgttgat
      301 ttccaatcgt ttttggtacg ccttgactgc taccttgatc attaacttac tgctcactat
      361 ttcgacagta atcaaaatgg aaatgcggga agagcctgtc tttccttcag atttaaaaat
      421 gctgaccgga ttgtcagaac tattatcgat ggttagccca gtcttattaa tcgtgggcgg
      481 attgatcctt ctcctattat tgattactag tattattgta caacgtagac tacaaaaaca
      541 gtattccttg aagattcatt ggaaacgtcg atttatcagt atcgctgtat tattaggatg
      601 tttttcgggt gtctttttca ttaatcacaa aaattcgcct tctttcttgc tgtttaattt
      661 atttaaagta aacaagactt tctttaacca gcaaggcgcc gtaaaagata acggaccagt
      721 cattcaattc ctaaacaata tcgacattaa aatcatggaa gaacctgctg gctattcgaa
      781 agaaaaaatt aatggaataa tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac
      841 aagaagtgac tggttaaaca atcagacgat cgttctcaat ttaagtgaaa gcttcagtga
      901 tcctgcacgt gtccctaatc tgacagtacc tacagaccct attccttaca tcaaaagcat
      961 tataacagaa ggaacaggag gattgatgtt atccgtcggt tatggtggcg gaacagctaa
     1021 catggaatgg gaaggactga caggcttgag tatcagtaac ctgtctgctt cgttagttac
     1081 accttatacc cagctagtgg agcgtcagaa aataagtcca aatattaccg atctattcga
     1141 tgaaaaaata gccatccatc cttttacagc tgctctttat cgcagaaaac aagtgtttga
     1201 taaatttggt tttgacaggt tctattattt agacagtccg gataaattaa cttatacgga
     1261 taaaatagaa caaagcccac gcatatcgga tgattcagca tacaaagaaa ccttgaaagc
     1321 tttacaaagc aatacggaaa cgtcacaatt catccaatta tccactatgc aaaaccatat
     1381 gccttatgat gattactata gtgaattgaa ctacactgct gaaggaactg ctgtcattcc
     1441 taatagaaga aaagaattac aaacttatat gcaaggcctt cactataccg atacagcagt
     1501 aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg attacctttg ttttttatgg
     1561 tgaccattta cccgcaatct atagtgggaa tgattcgaaa aaatatggat tagaacagca
     1621 ccagacagat tatttcattt ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa
     1681 taaagaagtt gttgctccta ataactttgg tgctatggct cttgagcatg cgaatatcaa
     1741 agtaactcct tactatgcgt tgttgacaga agtatctaat catctacctg cattaacaat
     1801 tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa caggtctttg ttacagagga
     1861 tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa aaagaaattc tagaagatta
     1921 tcgtctgata caatatgatc ttgttgcagg taaccagtac agtgcaacat gggcgacaca
     1981 aaaaataaaa gactaacaaa taaactgagc aaggggttgt gataaaagac atgtcacaac
     2041 cccttgttta tctcattaat ttgcttgtcc ataaacgatt tgattaaagc catttttcaa
     2101 ttcattattc gctgtctcgt tgaagtaaag ataattatcg ggattcccct gattttgttg
     2161 tgtcaccaat acttttggct gattttgctg aaacccgaat agatagacgt gctttttcag
     2221 atatggttct gtctctgcat cggaatagac ggctgtgatt tgatagtctg cttgacctgt
     2281 cccactttct gaccattctg cagaaacagg cactccacca atagccatct tccactcccc
     2341 gttcaaaata gcttgaggga ctttaagacc atataagtcc acactcattt gattcgtata
     2401 acttttgtat tgttgtccca ttgtttggcc ccatttggtc ataaatgcct ctaattgtgc
     2461 ggatttgtct gcattccaca gtgtttgttc aacttgttcg ctgctttctg aagaggaaga
     2521 tgaagcctct acagaagaac ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga
     2581 ctctgacgaa gtagaggtat ctgctgtttc tgtacttgct gcagttgact tgctctctgt
     2641 gctagttgtt gtagtagttg caactgttgc tttcgctgtt tcttttgttg attctttttt
     2701 cgattcattc ccgttggtac aaccagctaa caaaaaagct agaccaattg ttactaaaat
     2761 cctttttttc aattaaaaaa ctcctttcga ttactcccac ttctttattc tatcaaataa
     2821 gcaatgtgat tttattgatt tttcgtattt tctcatcaat ctttcaaatc ttctaaaaac
     2881 tgagaagcac ccgctgatgt tgtctgtaat acatcagcgg gtgcttcttt tattttaatc
     2941 ttcgtgattt tccaatttac ctcacgcttt atatttgtat ttgaagaaac atagactttt
     3001 agtcatcatc gtcttcctca tccaaatctt gtcggacagt ttttccttgg agtctttgta
     3061 cttggtagat gatttcttta ttgaaactta aaagttcaac aagcaattgt tgcaagccat
     3121 atttttcttc tttttcagct aatttgattg cccgtgtgat aaagacattt tggaaatcaa
     3181 aatcttttgc aaagttttcc aacattgttg aggctggtaa atatttgttt tgaccatgtt
     3241 cgtaaatttt tccataacgc atgacttctt ctaaagtaga cggaacgatt tcattttcaa
     3301 tcactaactc atgagagatt ttattgaacc atctcgcttc ttgatcagca tattctgaaa
     3361 aaagggtact taattcttgc gaagatgtac ccaaaacata caattgtgct tggatcaatt
     3421 tcatttcttc taatttaaaa tttgctaata catgccccac catggcgcca gcagttggtt
     3481 tatgatggtc gatatctgct tgttttgttt cttgaacaaa tttttcttcc actatattca
     3541 ttgatttacg cctcttttac ttttgctgat tggacttcat aacctaattt ttccacaact
     3601 gcagctaatt gttcagggtg gacttcatct ggatcaaagt ttgctttgat tttgctcgca
     3661 ttaaacaaaa ctttcgtatt gcttacccca ggctgttgag aaaccgcatg ttcgattttt
     3721 tgcatacagc ttggacaagc tagtgtatct aattgcataa taattttttt catcgtagtt
     3781 cttccttcct ttttcttgat agtcttattc tagattacag cttccataaa aaaattgatt
     3841 cttatcaagt tttacgattt ttcttctttg ctttcgtgtc caaaatacaa taaacgcatc
     3901 gcgttaaaga taacgatcaa gatactagct tcgtgaacga acatcccact agccatgtaa
     3961 ataaacccgg caaataagcc gttgagtaaa aataagacga caccaatggc aatcactaca
     4021 ttttcacggg tatttaaaac agttttctta gccaagcggt aggcatgaac caacgattca
     4081 aaattagatt gcatcaagac aacgtcactc gtttcgatag caacatccgt tcctgatccc
     4141 atagcaatcc cgatatcagc cgcagccaaa gagggagaat cattgattcc atctccaata
     4201 aaagcaacgc gcaatccttg ttcttggtat tttttcacaa attgaacctt ttcgtctggt
     4261 aataattcag catggacttc atcaatgcct aacatatcag ctacataatc agcagtcatc
     4321 tgattatcac ctgtcagcat cactaactgt tttgctcctg cttgtttcaa cttagctaat
     4381 tgttcagcga cttctggtcg aatcacatct gacacaccta tgatttgagt gatttttttg
     4441 tcgattgcta caataatgac cgagctgcct gtttgttgta attgatgtaa atcttgtact
     4501 tgctttggtg tgagagaaag tttgtgagcg gcaatcaatt tggtgttgcc cgcacaaact
     4561 gtatgaccat caatctcagc tgtgattcct tgtcccttga ttgtctgatt atctttgact
     4621 gctaattgtt gaaaatcaat gtctttctcc tgagcataat ggatcacggc gcgccccaga
     4681 ggatgatcgg ataacgtttc taatcgagcc actaaagcta agctgtcccc ttcatcctct
     4741 gtatagttct tgatagtgga tacagcagta gttcctttgg tcaatgtacc agttttatca
     4801 aagacaaaag tatcgatttt ggaaaaagta tccattgctt caccgccttt gactaagatg
     4861 ccccgtttcg ctccattacc gattcctgct acattcgaaa caggcgcacc aatgactaaa
     4921 gcgccaggac aaccaagaac caaaacagtg attgctaaac gaaaatcacg actaatcaga
     4981 ccaacaacca acgcaataag taaaacggtt ggagtataat actgagaaaa tcgatcaatg
     5041 aatttttccg catgggattt ggtatcttgt gcttcttcta ctaattcgat aattttggca
     5101 aaggtcgtat catctccgac cttagttgct tcaatcgtca cgtagccatc tgctaaaatc
     5161 gttccagaga agacttcatc tccactgatt ttgacgactt gtcgtgattc accagtcact
     5221 gcggcttcat ccgcataccc tttcccgtca atgactcgtc cgtccacagg aatagctcca
     5281 ccagttttta ctaagacgat atcgccttct tccacgtcat cgatttccac ttcttcagtc
     5341 gttccatctt cttggacaac actagcagta gatggggcca tctcggtcaa agccttgatt
     5401 gactcccgcg ttttggtcaa tgttttttgt tccagatagc taccaaacaa aaacaagaat
     5461 gtaacgatag ctgactcctt gtattctccg atcataaagg caccaagcac agcaatagta
     5521 acgagtaaat caattgagat aactttgact ttcactgcct gatacgcatg gatcataata
     5581 ggtatgaccg caataataga agctatggct agagcaatgt tatagaacaa gacaaaacca
     5641 gtaaattttc cgataaaacc aaaaataatc aacacagctg ataaggcggt gatatgattt
     5701 cgatgctctt ggatccactt ttgtaatttc atctttctcc ctcttttcct ttcattctta
     5761 cgagactaag tatagatact tatccatcat aaaaaattga ccactatcaa gtttagtcaa
     5821 aaaggcggtt ttcttttggt agaatacact acaaaaaata gttagcaaga agactcacta
     5881 aaagtgatct tgccaactat tgtcgtttct atcgaaattt tcattgaatg attttcctgt
     5941 acagcataca tcggagatct atttctttat ctaagatttt tatcttgcta attttttcct
     6001 ttagtctttc aaatccacgt cgttcataga attgatacgt acagccacta tctgtaaaaa
     6061 ggaatatttc ttttcctttc tctctttttt caaattctgt tagcaacttg gtaccgatgc
     6121 cttttgtttt caaatcagga tttgcagcta aaaaaatgat ctctccatct ggcgaatgct
     6181 tctttaaata tgctgcaagc atttgttcat ttgctgtctc gtaagctcct ttactttctt
     6241 tagcaaatat attttgtaaa aaatcgaata tcttaacata tatttgctgt ccaaaagaac
     6301 gatgtttctt tttttctcct tttatctccg ccagcaaaac acctgccaac tcgtcctctg
     6361 catacaaagc aagaatttga gttgctcgat tcaactccat atacaaaaaa tatcttccgt
     6421 aaagattcag cagaagttca ttttccatat atctctcaaa gttcattcct tcaacagcaa
     6481 aatagatagc ttttttgtaa tctttctttt ctaagggttt aaaagttagt ttcatcattt
     6541 cgcctcctca acttcaatga ttgtgttata cttacattat aatagacaca gtgtctacta
     6601 tctataatca atcatctata agtttgtcga ttaagcgaca gctttgcttt gatatgtcgg
     6661 ataaggagat aggtaaatga aaaagacaga tagacgcgtc aaaaaaacag aaaaagcttt
     6721 ggctgaaaca ctatccacat tattagtaaa taaaaagatt caagcaatca ctatacgcga
     6781 actaacagaa acggcagatg tacatcgttc gactttttat acacattata aggatatcta
     6841 tgatctatat gat
//
LOCUS       DASJON010000152         6851 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJON010000152 DASJON010000000
VERSION     DASJON010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.154"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            527..721
                     /locus_tag="U3989_002241"
     CDS             527..721
                     /locus_tag="U3989_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715674.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            843..1161
                     /locus_tag="U3989_002242"
                     /pseudo
     CDS             843..1161
                     /locus_tag="U3989_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1405..2235)
                     /locus_tag="U3989_002243"
     CDS             complement(1405..2235)
                     /locus_tag="U3989_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN1715675.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2443..3777
                     /locus_tag="U3989_002244"
     CDS             2443..3777
                     /locus_tag="U3989_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1715676.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3818..4729
                     /locus_tag="U3989_002245"
     CDS             3818..4729
                     /locus_tag="U3989_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1715677.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4716..5579
                     /locus_tag="U3989_002246"
     CDS             4716..5579
                     /locus_tag="U3989_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1715678.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            5576..6192
                     /locus_tag="U3989_002247"
                     /pseudo
     CDS             5576..6192
                     /locus_tag="U3989_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6194..6712
                     /locus_tag="U3989_002248"
     CDS             6194..6712
                     /locus_tag="U3989_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1715679.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2061 a         1186 c         1372 g         2232 t
ORIGIN      
        1 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
       61 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
      121 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
      181 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
      241 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
      301 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
      361 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
      421 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
      481 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
      541 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
      601 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
      661 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
      721 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
      781 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
      841 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
      901 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
      961 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     1021 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     1081 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     1141 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     1201 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     1261 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     1321 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     1381 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     1441 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     1501 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     1561 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     1621 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     1681 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     1741 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     1801 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     1861 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     1921 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     1981 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     2041 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     2101 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     2161 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     2221 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     2281 gaaagattaa aaaattcaaa gaaagcgctc gcgaattata taaagtattg gtatcattaa
     2341 taattttttt agaatcatac cttataaatt gttattttca gaacaaaatg atatcgttat
     2401 attaaaaccg gcatcggtta cattaaaatt tggaggtaaa atatgaaaaa attattgttt
     2461 acagggttgc tggcaagttc agcattgttt ttattaggag gatgcggatc aagtaatgca
     2521 agcgatgcca gttcaagtgc agcaaattct tctgaaaaaa caagtgatgg aagaaaaaat
     2581 attgatttct ggtccttttg gggatccggt gcacgaaaag aagctatcga agaaatcatt
     2641 gaggatttca atcaatcaca aaacgagatc catgtaaatt ataagtatca gccatggggt
     2701 gacatctgga ctaaatcttt atctgcaata acagctggaa atgcacccga tgttattgta
     2761 caggacataa attccgttgc ccaaagagca gaagcacagc aagcaactaa tctctccaaa
     2821 tatgtagaaa aagacacttc taaggatttt tatccgcagc tttgggatac agtagaatat
     2881 gaaggtgatc cttatgcgat cccattcaat acagatacac aagtcatttt ttataataaa
     2941 aagatattta aagaagctgg aatcagtgaa caagatctac cgacaacttg ggatgaatta
     3001 gaagaaacag cacgtaaact agataaaaaa gaaggaaaag aatttacacg catcggtttt
     3061 tatccattat ggaatttagg ggctgatgta tgggcgctaa acgcagataa cgggactagt
     3121 tggtttgaca aagatgaaaa agtcaagatt gatactaaaa ataaagtgga agccttggag
     3181 tggatccaag actggcaaga ttattatggg aaaaatacga tcaataaatt agaagccgaa
     3241 tttgggtctg gtgtatctga tccatttatt tccggattag tagccatgcg tgcccaaaat
     3301 atcaattatt actctagttt gatggaaaat gcaccggatg attttgattt tggcgtgatg
     3361 cagctcccag aaaaagaaga aggttcaggc cattggtcgt ggggtggcgg attcgtcttg
     3421 gaagtaccat acggagcaaa agatcctgag gcctcttatg aattcatcaa atatctttct
     3481 tctccagaag tacaagaaaa attcggcgaa aaaagttttg atatcatggc gagcaagaca
     3541 gccaatgaga atttagtgaa taatgacaac ctaacggata aaggaaaaat gatctatcaa
     3601 atggcggacg aaaactttaa aaatacggtg atcacaccag ttcctttaga tgctccggat
     3661 tacacgacat tagtgaatga acagattgac cagattcttt taggaacaaa atcaccaaaa
     3721 gaaggattgg cagatgcaca aaaagcagta gaaaacttag tcaagcaaaa tcaataatcc
     3781 atttttgact gtctaggtac agaggaggca agaaaatttg aagcgtttat ctatccgaaa
     3841 gaaaaaagaa gcaagatggg cctatttatt tatctcgcct tttattatcg ggttcttggt
     3901 atttatgcta gggcctatgc ttttttcagt tattggtagt ttcaccgatt ataatttgac
     3961 atccaagatg aatttcattg gtctagcaaa tttcaaaaga atgttcgttc acgatgattt
     4021 attttggaaa tcgttataca acaccgttta tttcgtcatc ttcaatgtac cgctgacgac
     4081 gattttcagt gtgtttttag ccacagtact caatcaaaaa atcaaaggaa tcagtgtatt
     4141 ccgtacagct ttctatttac cggcagtctt atcaggagta gcagtctaca tcttatggat
     4201 gcagctgctt tctccatctg caggattgat caacacattt ctgggctggt tccatatccc
     4261 tggaccagct tggctgtttg atccacaatg gacgaaacct gcgttgattt tgatgaaaat
     4321 atggagttct ggaggagcaa tgctacttta cttagctaca ttacaaaatg ttcctagatc
     4381 tttatatgaa tcagcagaaa tcgatggagc aggcgtttgg ggaaagttca aaagtattac
     4441 tttgccatta atcactccta ttatttttta cgatgtgatc acctctacga tcggttcttt
     4501 ccaaattttt caagaagctt atgtcatgac gaaaaatgga acaggcggac ctgcaaattc
     4561 tttgcttttt tataatctgc atatgtggaa caaggcgttc gtagcttttg atatgggtta
     4621 tgcgatggct atgtcaatga tcctgtttct gatcgtgttg gttctgacgt ttgtcaattt
     4681 gaagcttgca cctaagtggg tctattattc tggaggtgac ggcaaatgat ggattatttc
     4741 aaaaagacca gtgtgaatcg gcaaacaaaa aaatactaag ttatgtcctc atgacagtaa
     4801 taggaatcat tttgatcatt ccattgcttt ggatggtgtt tacttcattg aaaccaatgg
     4861 aagaaatcgt tcgttatcct ccgacctttt tcccagaaaa aatcgtatgg gaaaactatc
     4921 tggatacgat tgccgccttt cctttttggc gctatgcaag aaatacatta ttcatcactg
     4981 tgctagtagt gatcggtaat gtcctttcaa attcttttat tgcttatgga tttgcgaagc
     5041 ttgattttcc tgggaaaaag ctgatgtttg ctttggttct ttctacgatg atgatcccgg
     5101 ggtttgtaac gatgatccct caatatgtat tattctccaa gatcggctgg gtaggtactt
     5161 atttgcccct gatcgttcca tcgttcttcg gcaatgcgtt caatatattt ctgatgcgtc
     5221 aattttactt atcgatcaat aatgaattga tcgaagctgc tgaaatcgac ggagcgaatc
     5281 atttgtatat ttggagtcat ttgatgcttc cattgacgaa acctgccttg attaccattg
     5341 cgatcaattc cttcaacgca gcttggaatg atttcttagg tcctctgtta tatatccaag
     5401 atcaagaaaa atacacgtta cagattggct tgcaagtttt tcagaaccaa gcaacgacac
     5461 aatggaatta tctgatggca ggagcaacac ttgtcttgat cccgacgatt ttgctgttct
     5521 tttttgcaca acgttatttc attgaaggga tggatttgac aggaggttca aaaggatgat
     5581 gaatcgatcg attggtaaaa aagccaatga ttatttctta gaaatcattg aatggattcc
     5641 acgtcttatc catttacagc tactatggtt cctgtgcgtc ttgcctgttt tcacattagg
     5701 aactgcaagt cgaacagttc tgtatatgat ttatcattac aaaaaaatga agagaaaaag
     5761 ctcggttcac tattctggaa aaagtttcgt gagaacttct ctctttatgg aaagcaagat
     5821 agtttagcaa gtgtttattt tcttcttcta ttgatcgatt atcagatttt ccgctattgg
     5881 ggtgggggaa ttggctatac attgatgtat actacgttgt ttttgcttct attaagcgcg
     5941 cttctcttta gttacaaaat cttgcttcaa ctggaacaaa aagaagcttc ttggattact
     6001 gcatttttct tgttttttaa tgatttcaaa agcgcgcttt tctatttggc aggcacgttg
     6061 atccttcttg tcgcattctg gtttgcagga ccaatctatt ttgctttatt aggtttcagt
     6121 ttcttgtttt attttcaagt cctgcttttt atccatcgta cgcaagaaaa ggttcttaaa
     6181 aaggaggaat aacatgttgc gtttgattgc taaaatcgat gatagtatta tcaacaagtt
     6241 cgccaagcaa cggaaaacgg atagtcgaaa aatcaatttt ttgaaattta tcagtaatat
     6301 ctctaatggt ggttttattt ggttgctcct tgctgctgtt ttagtcttga tacctgatac
     6361 tagagaatat ggtctagcca ttcttatcgg cagtttgatc tatttttttg ttggtttgct
     6421 ttttatcaaa aaaacgacaa gacggaaaag accttatgaa aaaaatcgtc ggcttgtcct
     6481 ttacacaagt aaaccgaaga cttcttcttt tccttcagga catacagcgc tagccttttt
     6541 atcagtagct gcgttgttcg aattaagtcc agttgcagga atattagcct ttttatttgc
     6601 ttgtttgatc gcttattcga ggattttttt acttttacat tatcctactg acattttagg
     6661 aggagctatt ttaggaagtg ttattggcat cctatgcgct gccgttatct gaattaattg
     6721 tacgaataaa gcatgagtgc gtaaatgcgc atttgtgctt tatttttttg tttcgaggaa
     6781 agcgtagttg tttattcatt tgtcttaata acttaagttt tgttaaatga gaacgttata
     6841 aaattttata a
//
LOCUS       DASJON010000153         6744 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJON010000153 DASJON010000000
VERSION     DASJON010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.155"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="U3989_002249"
     CDS             278..787
                     /locus_tag="U3989_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1715680.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="U3989_002250"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="U3989_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1715681.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="U3989_002251"
     CDS             2322..3302
                     /locus_tag="U3989_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1715682.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="U3989_002252"
     CDS             3424..4842
                     /locus_tag="U3989_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEN1715683.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="U3989_002253"
     CDS             4913..6382
                     /locus_tag="U3989_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1715684.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..>6744
                     /locus_tag="U3989_002254"
     CDS             6392..>6744
                     /locus_tag="U3989_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1715685.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVF"
BASE COUNT         2307 a         1027 c         1366 g         2044 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttga
//
LOCUS       DASJON010000154         6464 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJON010000154 DASJON010000000
VERSION     DASJON010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.156"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(135..1046)
                     /locus_tag="U3989_002255"
     CDS             complement(135..1046)
                     /locus_tag="U3989_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1715686.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1447..2025)
                     /locus_tag="U3989_002256"
     CDS             complement(1447..2025)
                     /locus_tag="U3989_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1715687.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2045..2713)
                     /locus_tag="U3989_002257"
     CDS             complement(2045..2713)
                     /locus_tag="U3989_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1715688.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2728..3798)
                     /locus_tag="U3989_002258"
     CDS             complement(2728..3798)
                     /locus_tag="U3989_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1715689.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3969..5333)
                     /locus_tag="U3989_002259"
     CDS             complement(3969..5333)
                     /locus_tag="U3989_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1715690.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5330..>6464)
                     /locus_tag="U3989_002260"
     CDS             complement(5330..>6464)
                     /locus_tag="U3989_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715691.1"
                     /translation="RVKELNDSLNEFHLKIKSGRLTGPESQISYFYFQLFWFGRPYLE
                     NKKEYSDLSGNTFLDLLQKEFSFAITEEGRLKFEIWLSIMKKRMRCTKKSKPDYSNLP
                     VGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNETLVFYLFMISNFTLDTHNSHVQEFLI
                     SEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEEISQKIALTVMQNHAHFAYFRGWMNIF
                     GQEGLKQRLTEVEDKQFLVLCERLVRQTFAIVDFTEVDEENARFELFARYASILRTLI
                     KQLELRLQIAYDLPYERLMADIVKEIIQEQLLNTIKYDLVEYEKEKQYDIILTSQLKE
                     YPSQKSAKVFVFTSNEYNYDFPYLNQFLKECYLEKLNLRMNKT"
BASE COUNT         1866 a         1308 c         1043 g         2247 t
ORIGIN      
        1 tccttgtgac tcagagttga acggcaggct cacgacctct tccttaaacg gtgtttaaaa
       61 gcttgcatct gcgataataa gcccggccaa acgaaaaata tgaggagcta tttttgtttg
      121 gctgggctta tttattactg aaaataagtg gtcaatgctt gtgtcaaact tgcagcaatc
      181 tgctgctgat aactgtctgt tactaattcc tttaaatcac tatcattatt catataaccc
      241 atctcgatta aaatagaagg ttgaagattt tctcgaagaa cttgatagtt cccagtggag
      301 attcctcttg aatccagagg cagatcagtg aaggagtcag caattgtctg tgctaatgtt
      361 tcctcttgtc catagtaata atatgtcgtg attccagttg cttcattcgc atattccgta
      421 gagtctgcgt gtaaactgat aaagacatct gccttttttt cttcactgta gtcgcatata
      481 tctccaagct gtactaattc atctgtagta tgggtcaaat aaaccgttgc tccagcatcc
      541 tctagcgcct tttttacttt tatggctgta cttaatgtta tatctttttc ttctgtttgt
      601 tcatcattgc ttaatgcccc agtatcattt ccgccatgcc caggatctaa tacaatcact
      661 gcatcagata gctcttccgg ttttgtttgt atattctttg tgactttggt tccatcttcg
      721 agggctaaat atccagtata gccatcattt gtcttgatct gataagcatt ttttgtttca
      781 gaaagaaact ccgccagctc tccgccgttt atagttgcaa gtggggtagt tagcgagtca
      841 tctgtatata gtgtacggtc attttttccg attttgatta atgtctcttg ttctgtatca
      901 tcaaatgatt cctttgtctt tgtaaccgta ctgttcgtac tgctgaccgt ttgtccaact
      961 atcttttttt gaccaaaaac atctatttga aaacagataa taatgataat gattgcaaat
     1021 acaaaagctc caattatttt tttcattctt gactctcacc tcgtagatcc agactaaggg
     1081 ccaaaactta aaatcagctt aagtaagcaa aaaaggggct gtaaaagata taaccgtaga
     1141 aaataacatt actgctaaaa tgattttttt catttttggc atatcctgaa atctcctttt
     1201 gactattcgt ggtgattcat ctacccaata aatagtttcc ttcattctac aaacaattaa
     1261 tttcttcgtt attaaattaa aacacttcct ggaacaccga cataataaat aaaaatatac
     1321 cctgataatc atgagagaat aacaaatttg tatttatgaa aaactaccta tataaagaag
     1381 caagatataa cgatcttcgt catatcttgc ttcttcacct aactattttc tccctatata
     1441 acagctttac gcttctccta atacaccatt caaatataaa tcgatcaatt gcgccacatt
     1501 tagcggttct tccacaggtc ttttcgaccg agtgatgtaa ggtgctaaca ctaagaaaaa
     1561 atgccttgct gcgatttctt ttgaaatccc atttcttagc acagattggt ttgctgaaaa
     1621 gatttcttca aaaggttcac gataattttt cttccataga aagaaaagat gatctcttgt
     1681 ttcctttgaa agattgcttt caagatcgtg aagcatcaga aacaaatcga ccagatgtgt
     1741 ctcaatcaaa aactgcgcca tttttgtcaa ggttacttga aatcccgttt cgcttgtttg
     1801 aacgatttct ctcaaagctt ttccaacatc ttttaagtgg ttttctaaaa ctgccgtata
     1861 tagtttttct ttatcaccga aataatgata gagattgggc tgggtgatcc caactttttg
     1921 tgcgatttct cttgttgaag tgccgagata tcccttggac ataaacaagt cagtcgcttc
     1981 tttcaaaatt ttttgctgtg tatctttgtt gtatcgatgg accatttttc cacctgcttt
     2041 ttggttattc tttttctttc aatacaccat cttccatcat aaatattcta tcgcatttgt
     2101 caatcagccg tgtatcgtga gtaaccatga tcgttgcttt atttttgtct tttgattctt
     2161 ttgccaagat atcgactact tctcgtgcct tttctgaatc caaactagca gttggttcat
     2221 ctgctagaat aatggacgga tcattgtaca atgcacgagc gattgcgacc ctttgccgtt
     2281 ctccaccaga tagttcttct ggatacttgt tgatcaactt atccacgcca agctgctcaa
     2341 ataattcgtt tgcttctttt tgacgatgtt catgtttaat cttatctact aataataact
     2401 gcttttttac tgtcagaaaa ggaaccagat tggaagcttg caaaataaag ccaatttctt
     2461 taaaacgtag tttaacgcgt tcgctttctt tttgattact gaattcttga tcattgataa
     2521 atacagagcc actgctaggc gtctgcaatc cgcctgccaa agtcaaaaaa gtacttttac
     2581 ctgaaccaga aggaccgatg attgcaacaa attccccttt atcgatactc atggttgttt
     2641 ctttcaatgc tttaactact tggtcgccat cttggaaact tttttctact tctttaaatt
     2701 ctattgcttt catgttcttc ctccttctta tccaattgcc ttcaacggat cgatttttac
     2761 gattgtccga acagaaaata gtgcgcctaa cacagctact actaacatca acaagctaat
     2821 gacgccaaaa aacagccaat tgctttggaa tggaaccgca tcaggtagaa caagggatgt
     2881 tcccactgtt cctaatagac caagaagaat accaacaaat gataaaatga atgtttgtgc
     2941 cactacagaa cgcgcaataa atcctcctgt gatcccttgt gctttcatta caccaaagat
     3001 attaatcttt tgcatcgtca gtacataaat gaaaatcccg atcacaatag ccgcaataac
     3061 gatcaaaaat ccgatcataa atccaaaagt caatacttgc gcattatatc caggtaattc
     3121 attgatgaat ttagaaattt tgatctgttc taaatcttca ggtaaattac tgattttacc
     3181 tctgacaact attgcgttaa tccgtgcatt ttcacttgta tcttcttttt cgaagcggat
     3241 ttcttggtaa gcattgatgg ttgtatagag aactggtgag acattgaatt tggcatgatc
     3301 cgtaaagcca gttagtttaa acgtcttgtc actaccagat aacttaactg tatctcctat
     3361 ggctagacca tattcttctt tcaaactaat atcgcctact gcctcatcat cgttatcaaa
     3421 agcttttcct tcaacaagat tgggcatgat gaattgattt ttatcgatac cgaagaagct
     3481 aacattgatt tttccttctt ctgtactgtc tttactagtc acgactccag gtgtttgggc
     3541 aagatacgcc actttcccac cttcgacttc ttctgctatc ttcttagtaa tcatggacat
     3601 ccctagattt gaatttgcat ctttcgaaag cacaatcgaa tctgcctccc acttgtcgac
     3661 tgctgttcga ttatcttgag ctaagccata agctaaccca gtcaaaaaaa ataccaggta
     3721 ggcgatcaaa aacatcaccc ctgcaactaa cgcataacgt aactttgaat gcataatttc
     3781 atttaatgct aaaaacattt aactcctcct cctgaaataa tcattttata caacaatttt
     3841 atcacttgat aaatttttgg cgaatattat gctcaaaaaa ttcttccgct atttcattat
     3901 atgaattttt tattctcgat tgattaatat ttattatatc cccacacaat aaaagaccgt
     3961 gatatttctc acagtctttt atctactcta ttgctatttg ctctcttagc tagtacttta
     4021 aaagctaact cattgaggta gtgaaaacga gaggtgtatt cagacagaaa tccaaaatcc
     4081 tcaactgcta gctgtggaat cgtagttttg tgtttcacga tatccgcaag caggttcaaa
     4141 tctgataata agaattcttg gcttatcagc tgtccgccaa ttatttctaa tgtatccgga
     4201 tggtagatca gctttgcatt tactgttttg ttttcttcgt cgtattgtgg aaatagatca
     4261 aattctcctg aacacgaacc ggtattttgt tccaagaatg gcgcttcatc ttctgtcagc
     4321 cctacagttc ctaaaaagta gtctataatg actgctgctg ttatcttttg tctgaggtcg
     4381 taagcggttg gctgaccact caaagttagc gctactgctc ttgccatatg tacagctcga
     4441 gtaactaaag gtaaaaagag atgccgttta tttttgtcaa aactgactgg gacgatatct
     4501 ccaatcgcat agatgtccgg atcacttgtc tgcagatatt cattgactat cacagtctca
     4561 tcctcataca aagccacttt ttcttttaac aatgtcgtat taggacgagt ttgggcagaa
     4621 aaaatgacca tatctgcctt tattgtttga gacagaagtt gtacggatac tactttctct
     4681 tctgattttt tccaatcgat caagaattca tttggataaa aatttatttg cttagtttgc
     4741 agctgatttt gaaaactgtt gatcatatcc ttatcgaaat atcttgataa aacattcggc
     4801 atataatcaa ctagatgaat aatgtatcct ttatttctca aagcatctgc taattccaat
     4861 ccaatgtaac cagcaccgaa gatcactact tcttttgctt cttttaaagc ggcaagtact
     4921 tgcttagatt gcactagatt tttataggtg tagacatttt cagcttcaat ttgtttatac
     4981 aaggtgacag gagaggaacc tgtcgctacg attaatttat cataggttat ggtctcgttt
     5041 tttccttctt tggatgcgag gatgagctga tgtttgtctg gatcaagttc agttactctc
     5101 cattcggtga tcagttttgc cccactttct tgaagagtcc gcatgttatg ggcaacttca
     5161 gctaaactac gtatctttcc ttttaaataa aggttgattc catttgaaac atagcctagt
     5221 ccatctttat gatcttcatc gatcagtaaa acttctgctt tcggttgaag ttttcttaga
     5281 tttagggcag ctgtgatgcc ttcatgggaa gcaccaatga tcacgatttt catgttttat
     5341 tcatccttaa attcaatttt tctaaataac attcttttaa aaattggttc aaatagggga
     5401 aatcataatt atattcgttg ctagtgaata caaaaacttt agcggacttc tgtgacgggt
     5461 actctttcaa ttggctagtc aaaatgatat catactgctt ttctttctcg tattcgacta
     5521 aatcatattt gatcgtattc agcaattgtt cctggatgat ttctttcacg atatccgcca
     5581 ttagcctttc ataaggaaga tcataagcaa tttgcagacg taattctaac tgcttgatca
     5641 gcgtccgtaa aatagatgca tagcgagcaa ataattcaaa gcgggcattt tcctcgtcta
     5701 cttcagtaaa atcaacaata gcaaatgttt gtctgaccaa tctttcacat aaaaccagga
     5761 attgtttatc ttctacttca gttaaccgct gttttaatcc ttcttggcca aaaatattca
     5821 tccatcctct aaaataggca aaatgagcgt gattttgcat cactgtcaac gcaatctttt
     5881 gcgaaatctc ttctggaaaa acataaaaag agaaatattc ttctatcgat tgtttaaacg
     5941 ttcgattcaa ttgagctact acgtcgtctt tgtatttttc gcttatcaaa aactcctgga
     6001 catgcgagtt atgtgtatct aatgtaaagt tactgatcat gaaaagataa aaaaccaacg
     6061 tttcattttg attccaagga aaggcatatt gaaagaagta acgggtcaaa aatgtatgga
     6121 ggtccaataa aaattgtgtc gtttgaaaca atccaacagg aagattgcta taatctggtt
     6181 tggatttttt cgtacagcgc attcgcttct tcataatgga aagccatatt tcaaatttca
     6241 atcgtccttc ttctgtaata gcaaaactaa actctttttg cagcagatct aaaaaagtgt
     6301 ttcctgataa atcactgtat tcttttttgt tttcaagata gggacgtcca aaccaaaata
     6361 attggaaata aaaataacta atttgacttt ctggaccggt cagccgcccg ctttttattt
     6421 tcagatgaaa ctcatttaaa gaatcattta attccttcac tctg
//
LOCUS       DASJON010000155         6437 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJON010000155 DASJON010000000
VERSION     DASJON010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.157"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..608
                     /locus_tag="U3989_002261"
     CDS             <1..608
                     /locus_tag="U3989_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715692.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="U3989_002262"
     CDS             628..1656
                     /locus_tag="U3989_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1715693.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="U3989_002263"
     CDS             1653..2411
                     /locus_tag="U3989_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1715694.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="U3989_002264"
     CDS             complement(2497..3237)
                     /locus_tag="U3989_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1715695.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="U3989_002265"
     CDS             3298..4551
                     /locus_tag="U3989_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1715696.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="U3989_002266"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="U3989_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="U3989_002267"
     CDS             5222..5548
                     /locus_tag="U3989_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1715697.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="U3989_002268"
     CDS             5616..6329
                     /locus_tag="U3989_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1715698.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         2258 a         1084 c         1269 g         1826 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctg
//
LOCUS       DASJON010000156         6219 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJON010000156 DASJON010000000
VERSION     DASJON010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.159"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(111..437)
                     /locus_tag="U3989_002269"
     CDS             complement(111..437)
                     /locus_tag="U3989_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715699.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(483..845)
                     /locus_tag="U3989_002270"
     CDS             complement(483..845)
                     /locus_tag="U3989_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEN1715700.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(882..1096)
                     /locus_tag="U3989_002271"
                     /pseudo
     CDS             complement(882..1096)
                     /locus_tag="U3989_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1224..1559
                     /locus_tag="U3989_002272"
     CDS             1224..1559
                     /locus_tag="U3989_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715701.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1635..3632)
                     /locus_tag="U3989_002273"
     CDS             complement(1635..3632)
                     /locus_tag="U3989_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715702.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3730..4005)
                     /locus_tag="U3989_002274"
     CDS             complement(3730..4005)
                     /locus_tag="U3989_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715703.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4237..4971
                     /locus_tag="U3989_002275"
     CDS             4237..4971
                     /locus_tag="U3989_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715704.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5010..6152
                     /locus_tag="U3989_002276"
     CDS             5010..6152
                     /locus_tag="U3989_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1715705.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2115 a         1139 c          895 g         2070 t
ORIGIN      
        1 tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact ccctagtgaa
       61 tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac
      121 attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca
      181 atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct
      241 ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt
      301 aaaatctgtg ttaccaatgg tttcgatgac ttctctaatc cagatgtacc tgtcattttt
      361 gaaaaaatga tacctataat agccaagaca acgataacta taatgataaa cttcttgttc
      421 atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc ttatccctct
      481 tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc
      541 atcaaaatcg tacccatttg ttcatcttta tctcgatata agtagcccat aataacagca
      601 acagcaccaa atacaacagg aataaatgct aacgctacta ccatagaaat ccaaccaata
      661 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
      721 gtctgctcag tacttttctt tactgcgtct gctccagttg tctcatctgt ttcttgtagt
      781 gctccgcaaa actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa
      841 tacatgtctt ctcctcctaa aaacattcaa ttgtttatta gttaactgtt tactttatta
      901 aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt
      961 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     1021 ttcatttttt tgctaactcc gtttgagaaa gtcctttttc ttttctgtat cggctgagat
     1081 tacttacttt tttcatgatt ctcccctcct gttttattat atacaacaat atgatgtatt
     1141 tcaactcata aaatataaaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
     1201 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
     1261 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
     1321 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaaccgctc
     1381 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg
     1441 aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg
     1501 actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa
     1561 aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc
     1621 tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca
     1681 tcatctcttg ttacacttgt aaattgataa agaggaacta attcaactcc tgataatatc
     1741 aattctttta ttacttcatc tatccctgct ccttttgcta ggtagactaa aaaactacta
     1801 cttgctttca cattgtatag tctcttaata tccttagaag ctcgttcaag agtgaatcct
     1861 tgtttaacaa agtagcctct gttttctttt aaagcatttt ccacgctgtt attcccaaca
     1921 ttacgtagac gttcacaaac agtgttagta agttctttct ctgtcattag atatgttcct
     1981 tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg
     2041 ctaagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat
     2101 atcttaaccc attcataaga aaaatattga tatgtaaaat gaaatttttc cagaagtgga
     2161 aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag
     2221 acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg
     2281 tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa
     2341 gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc atagtaaaaa
     2401 ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca
     2461 tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa
     2521 ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt
     2581 gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa
     2641 ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt
     2701 tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta
     2761 aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac
     2821 ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg
     2881 tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt
     2941 tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt
     3001 tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg
     3061 ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc
     3121 gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca
     3181 accattttac catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct
     3241 agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg
     3301 atagataaaa ccttttctat gcttaagtat ctactatcat ctttactcaa atctactacc
     3361 ttccccttct catctttaac agtgttatcg acatccagaa taattactct ggtttccaca
     3421 aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa
     3481 tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa acacctatct
     3541 cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc tttatctaca
     3601 taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata aaattctgtt
     3661 aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt tttagtctta
     3721 tttgtttact caacctttat accgttccat gaaactatca atatcagatt ttttaaatct
     3781 aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt ttctaatcgt
     3841 tcctactgat acacctagat attcacttgc ctgctgatag tccatatact ctagagtgac
     3901 tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt tcacttgttg
     3961 ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc cccctccttt
     4021 cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc aaaataaaag
     4081 tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc gacatacaca
     4141 taatacctcg ataaaaaaga ggtgtcaata tctttgtaaa atttatttac aacacccctt
     4201 tttcgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc accaataaat
     4261 tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa gagtaaaaaa
     4321 ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga caaaaaaact
     4381 atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc ccgaattgct
     4441 gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga atatattttg
     4501 ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc agataaaaac
     4561 ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga tacatggctg
     4621 gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa gacttgtaat
     4681 aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga attattcatc
     4741 aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag tatagaagaa
     4801 ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat gtcttatccc
     4861 gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac gatttcatca
     4921 attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta gtggtaagta
     4981 ctccttgtaa actaagagga gatgatttca tggcaaaaat atcaaagtac aaaaagaaaa
     5041 acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga actggtgaaa
     5101 aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa gattatgacg
     5161 agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg acatttgaag
     5221 acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca acaatcgcaa
     5281 tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt aaactatcga
     5341 atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt
     5401 acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca atgcaatata
     5461 ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc
     5521 gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa gattttggta
     5581 acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga aagagtgaag
     5641 tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat gtcaataaaa
     5701 ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca agagcttcta
     5761 gacgagttat ctctttagat actgagactc tttctatcct caacaactgg aaattacagc
     5821 aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa catgttttta
     5881 caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta agatatattt
     5941 tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca catgctagtt
     6001 tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg catgaaaata
     6061 gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag aaaacaggtc
     6121 aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct ggtcatttct
     6181 agtatacagg caaaacaaaa aagctagaac ccttatata
//
LOCUS       DASJON010000157         6189 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJON010000157 DASJON010000000
VERSION     DASJON010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.160"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..355)
                     /locus_tag="U3989_002277"
     CDS             complement(<1..355)
                     /locus_tag="U3989_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1715706.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALG"
     gene            744..2510
                     /locus_tag="U3989_002278"
     CDS             744..2510
                     /locus_tag="U3989_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN1715707.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2612..3871
                     /locus_tag="U3989_002279"
     CDS             2612..3871
                     /locus_tag="U3989_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1715708.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            4018..5319
                     /locus_tag="U3989_002280"
     CDS             4018..5319
                     /locus_tag="U3989_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1715709.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            5322..6173
                     /locus_tag="U3989_002281"
     CDS             5322..6173
                     /locus_tag="U3989_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1715710.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
BASE COUNT         2024 a         1078 c         1231 g         1856 t
ORIGIN      
        1 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
       61 ttgtttctgg aactattgca atgattaaag gaaaaaacaa gcttccgaaa agtactttaa
      121 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
      181 aaataactat agctcggtca aatcctaaaa cagcttccag taatattcct agaccgctga
      241 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
      301 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatc
      361 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
      421 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
      481 tatacttcta cgttaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
      541 cgcttaagat aaattcagga cactaaatag tacaggaagt agattaaaac ttttaaaagt
      601 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
      661 cgataacatt tttgaaagcg ttgtcttttg tttctctatc acttatagtg atgacgtcat
      721 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
      781 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
      841 atatcaaaaa agtatatgtg ctttgtggag atccatatac cataacaact gaaaaatggt
      901 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
      961 ttgaagtgac gagcgaaaca aggcgcttac agtatgcgtt ccatgttgtt ggggaagata
     1021 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
     1081 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
     1141 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
     1201 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
     1261 tcttcggggg agatttgcaa ggtgtgaggg atcatttgga ttacttgacg gaccttggcg
     1321 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
     1381 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
     1441 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
     1501 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
     1561 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
     1621 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
     1681 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
     1741 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
     1801 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
     1861 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcattcacag
     1921 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
     1981 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
     2041 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
     2101 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
     2161 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
     2221 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
     2281 ctaagattat ttctgaaggt tctactcagt ggctaatagt atatgatcgt gaagataagc
     2341 tctatttcac acgggaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
     2401 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
     2461 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
     2521 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
     2581 cagcacgttg aatacacagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
     2641 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
     2701 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
     2761 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
     2821 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
     2881 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
     2941 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
     3001 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
     3061 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
     3121 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
     3181 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
     3241 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
     3301 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
     3361 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
     3421 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
     3481 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
     3541 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
     3601 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
     3661 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
     3721 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
     3781 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
     3841 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
     3901 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
     3961 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
     4021 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
     4081 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
     4141 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
     4201 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
     4261 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
     4321 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
     4381 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
     4441 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
     4501 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
     4561 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
     4621 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
     4681 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
     4741 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
     4801 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
     4861 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
     4921 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
     4981 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
     5041 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
     5101 ttctccatga tctatctgtt taacaatggt ggtcccggaa gcgtaggcgg aggagctggt
     5161 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
     5221 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
     5281 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
     5341 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
     5401 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
     5461 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
     5521 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
     5581 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
     5641 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
     5701 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
     5761 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
     5821 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
     5881 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
     5941 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
     6001 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
     6061 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
     6121 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
     6181 gtatcatgc
//
LOCUS       DASJON010000158         6148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJON010000158 DASJON010000000
VERSION     DASJON010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.161"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            26..607
                     /locus_tag="U3989_002282"
     CDS             26..607
                     /locus_tag="U3989_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715711.1"
                     /translation="MNEHFSVSFAENPSKYFRRLVFLLILSLLLTVAMFLLPEKIREE
                     QVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKTYQDMLDVFNDPELM
                     EELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRITAGKGGITDFDKEL
                     IPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1057..2106
                     /locus_tag="U3989_002283"
     CDS             1057..2106
                     /locus_tag="U3989_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1715712.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2103..2984
                     /locus_tag="U3989_002284"
     CDS             2103..2984
                     /locus_tag="U3989_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1715713.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2987..3757
                     /locus_tag="U3989_002285"
     CDS             2987..3757
                     /locus_tag="U3989_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1715714.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4036..4890
                     /gene="aroE"
                     /locus_tag="U3989_002286"
     CDS             4036..4890
                     /gene="aroE"
                     /locus_tag="U3989_002286"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN1715715.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5068..>6148
                     /locus_tag="U3989_002287"
     CDS             5068..>6148
                     /locus_tag="U3989_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1715716.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNY"
BASE COUNT         2055 a         1066 c         1340 g         1687 t
ORIGIN      
        1 agtagttaaa tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa
       61 tccaagtaag tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt
      121 tgcaatgttt ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc
      181 gtccatacca accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa
      241 acaaagccgg gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat
      301 gcttgatgtt tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta
      361 tccgatcgtt tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt
      421 gacatttatt ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg
      481 aataactgat tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat
      541 caaacaatta gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc
      601 taattgagac attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag
      661 aacaagtgat gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa
      721 attcaaaaat tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact
      781 tatcaaaacg tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg
      841 tattaatggt gagactttcc catagataca actttttaga gtgcatttct aaagcttgta
      901 tctatgctag acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt
      961 aaagacaatg accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat
     1021 aataaataaa aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca
     1081 gttgtagtaa ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc
     1141 gaaaaagaaa gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa
     1201 tacgtgagcc atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa
     1261 gaaggattaa aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag
     1321 agcaagctag ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga
     1381 atcgcaacac ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta
     1441 ggtgctgtaa cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt
     1501 aatgtaacag gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat
     1561 ttgttgccaa atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa
     1621 taccaagtag cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat
     1681 gcggtgcctt ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat
     1741 tttatctatg ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa
     1801 gcaaacaaag caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga
     1861 ctagcaacaa tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca
     1921 gaacttgcga gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg
     1981 gatacagtga tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg
     2041 aaggaaaaag ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac
     2101 aaatgatcgt atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt
     2161 tcatgactta tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag
     2221 gtgctgctgt ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat
     2281 taggcgtagg tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa
     2341 agataccggt tattttagca gggattttag tgatgtcagg attgaactca gttattttgt
     2401 ttgtgatgcg aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc
     2461 atcgctttgg gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca
     2521 tcttgattgc tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga
     2581 caggagacaa cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt
     2641 taggtttaac tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg
     2701 acggatatgc agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca
     2761 tcatcggtga agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg
     2821 tcggcagtat catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga
     2881 cttatctgaa gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga
     2941 agaaagcact gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt
     3001 gacaatcaaa aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt
     3061 ggatgaaatt aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg
     3121 tgctggaaaa agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc
     3181 gatctctttt aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct
     3241 ttctcgagtt ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa
     3301 tttgctactt gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa
     3361 tcaaaaagaa gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt
     3421 ggatactcca gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc
     3481 gacgatcaca gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa
     3541 aacagctaaa gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg
     3601 tctcatgatc acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact
     3661 ccaaaaaggg aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg
     3721 cgaccttttg cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca
     3781 gtagaaggac ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat
     3841 aaacaattaa acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta
     3901 aatagaatat tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac
     3961 ctagacgttt cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa
     4021 caaaggagga ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg
     4081 gcttctcaca gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat
     4141 gcggtgtatt tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt
     4201 cgaacttttg atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct
     4261 tatctggatg aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac
     4321 gaaaagggtc gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa
     4381 gaagctggga tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca
     4441 aaggcagtca ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga
     4501 aacaaccaaa cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct
     4561 tgtaagataa aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa
     4621 agctgtcttc tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg
     4681 ctcttagata taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct
     4741 agagaaacac gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt
     4801 ggcatgttga tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg
     4861 atccatgtga ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc
     4921 tcgcataaac ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt
     4981 ttttattaaa ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt
     5041 atcagtaaga aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat
     5101 aagaaggcga aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa
     5161 cgaacgcgta tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca
     5221 tttgaacatt tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa
     5281 caacaaaaaa ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga
     5341 agaaagcgat cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca
     5401 ggtatctcat tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg
     5461 acttcagaaa cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt
     5521 ggaagtgata cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg
     5581 gatggtcctt cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc
     5641 ccaggggtag gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg
     5701 cgtcaaactt tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc
     5761 aaatcgtttg aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa
     5821 aaagatctga atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat
     5881 gttcttttgc agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga
     5941 cgccaacagc aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc
     6001 cgtgcacctt atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg
     6061 ttcttgacga aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt
     6121 gtgccagtag acggttcgtg gaattatg
//
LOCUS       DASJON010000159         6114 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.162, whole genome shotgun sequence.
ACCESSION   DASJON010000159 DASJON010000000
VERSION     DASJON010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.162"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..179
                     /locus_tag="U3989_002288"
     CDS             <1..179
                     /locus_tag="U3989_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1715717.1"
                     /translation="ILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEI
                     FLSYVQEAFYSSLI"
     gene            complement(265..1359)
                     /locus_tag="U3989_002289"
     CDS             complement(265..1359)
                     /locus_tag="U3989_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1715718.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1548..2309
                     /locus_tag="U3989_002290"
     CDS             1548..2309
                     /locus_tag="U3989_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1715719.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2424..2864)
                     /gene="fabZ"
                     /locus_tag="U3989_002291"
     CDS             complement(2424..2864)
                     /gene="fabZ"
                     /locus_tag="U3989_002291"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1715720.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3084..3839
                     /gene="fabI"
                     /locus_tag="U3989_002292"
     CDS             3084..3839
                     /gene="fabI"
                     /locus_tag="U3989_002292"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN1715721.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3949..4962)
                     /locus_tag="U3989_002293"
     CDS             complement(3949..4962)
                     /locus_tag="U3989_002293"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN1715722.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5127..6047
                     /locus_tag="U3989_002294"
     CDS             5127..6047
                     /locus_tag="U3989_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN1715723.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1876 a         1212 c         1093 g         1933 t
ORIGIN      
        1 ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg aatcagacat
       61 ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg aattacagaa
      121 caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc ttaatttgac
      181 aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg
      241 attatttctg caataactaa ttatctattt tttattttca attaatttaa agcttgacca
      301 tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac
      361 acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta
      421 cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa
      481 atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc
      541 ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc
      601 ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa
      661 ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat
      721 taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc
      781 atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt
      841 caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga
      901 gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact
      961 catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa
     1021 aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc
     1081 gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa
     1141 taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac
     1201 ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa
     1261 aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt
     1321 tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc
     1381 ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt
     1441 ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt
     1501 ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata
     1561 aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa
     1621 acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa
     1681 gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg
     1741 tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat
     1801 tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt
     1861 actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc
     1921 ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca
     1981 acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca
     2041 gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa
     2101 aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag
     2161 atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg
     2221 caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca
     2281 atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac
     2341 aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg
     2401 ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac
     2461 tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc
     2521 attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat
     2581 ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg
     2641 ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga
     2701 ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg
     2761 atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta
     2821 ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa actccttctt
     2881 tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag
     2941 caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt
     3001 aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt
     3061 atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg
     3121 gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg
     3181 cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg
     3241 gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa
     3301 tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg
     3361 ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag
     3421 ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg
     3481 caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt
     3541 ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg
     3601 agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag
     3661 ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg
     3721 atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac
     3781 tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa
     3841 cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct
     3901 cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac
     3961 ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata
     4021 caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt
     4081 gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc
     4141 aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc
     4201 ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg
     4261 agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat
     4321 tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc
     4381 tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt
     4441 aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa
     4501 atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc
     4561 aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata
     4621 gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt
     4681 ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg
     4741 ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa
     4801 acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc
     4861 tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt
     4921 tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt
     4981 attttctcca cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc
     5041 ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag
     5101 aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag
     5161 actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt
     5221 tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc
     5281 tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata
     5341 agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac
     5401 atagtctttt aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt
     5461 tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg
     5521 caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca
     5581 tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa
     5641 ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt
     5701 tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac
     5761 ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc
     5821 gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct
     5881 ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga
     5941 acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag
     6001 cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga
     6061 tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DASJON010000160         6111 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJON010000160 DASJON010000000
VERSION     DASJON010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.163"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(254..541)
                     /locus_tag="U3989_002295"
     CDS             complement(254..541)
                     /locus_tag="U3989_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1715724.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="U3989_002296"
     CDS             complement(561..785)
                     /locus_tag="U3989_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN1715725.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="U3989_002297"
     CDS             complement(1240..1410)
                     /locus_tag="U3989_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715726.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="U3989_002298"
     CDS             complement(1425..1958)
                     /locus_tag="U3989_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1715727.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="U3989_002299"
     CDS             complement(1979..2923)
                     /locus_tag="U3989_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN1715728.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="U3989_002300"
     CDS             3560..3646
                     /locus_tag="U3989_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1715729.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="U3989_002301"
     CDS             complement(3767..4456)
                     /locus_tag="U3989_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715730.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="U3989_002302"
     CDS             complement(4463..4741)
                     /locus_tag="U3989_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715731.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="U3989_002303"
     CDS             complement(4701..5615)
                     /locus_tag="U3989_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1715732.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="U3989_002304"
     CDS             complement(5597..5977)
                     /locus_tag="U3989_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN1715733.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1620 a         1131 c         1028 g         2332 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtacatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagctcac taaagggctt ggcaagctct atatttgcta gccaaatacc t
//
LOCUS       DASJON010000161         6019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJON010000161 DASJON010000000
VERSION     DASJON010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.164"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1451)
                     /locus_tag="U3989_002305"
     CDS             complement(<1..1451)
                     /locus_tag="U3989_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1715734.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1772..2536)
                     /locus_tag="U3989_002306"
     CDS             complement(1772..2536)
                     /locus_tag="U3989_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN1715735.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2564..3262)
                     /locus_tag="U3989_002307"
     CDS             complement(2564..3262)
                     /locus_tag="U3989_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN1715736.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3274..4053)
                     /locus_tag="U3989_002308"
     CDS             complement(3274..4053)
                     /locus_tag="U3989_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN1715737.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4069..5013)
                     /locus_tag="U3989_002309"
     CDS             complement(4069..5013)
                     /locus_tag="U3989_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1715738.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5059..5838)
                     /locus_tag="U3989_002310"
     CDS             complement(5059..5838)
                     /locus_tag="U3989_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715739.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1645 a         1252 c         1004 g         2118 t
ORIGIN      
        1 tacattgatc cccccctctg aagtacagtt tttactgtct taaataaaat tttaaaatct
       61 aaccccatac tccaattttt tatatattcc gtgtcaagtt ttgctacttc ttcaaaatca
      121 gtaatatcac ttctaccact tacctgccac attccagtaa ttcctggctt aatagcaatt
      181 cttgcacgat gatgataatc atattcttca tactctgatt taaggcatgg tcgaaatcct
      241 ataaggctca tctttcctgt tagaacgttg aaaaattgag ggaattcatc taaagacgtg
      301 gatctaataa attgaccgat tcctgtcttt tttgttccat ctggaagtat tttatttccg
      361 ataattcttg gatcaaactc catcttaaac ataagattat tgctcatctt attttctttc
      421 attaattcta cttttctttc ttctgcatcc atatacatac ttctaaactt atacatcttg
      481 aacggtttac cattctgacc tatacgttct tgtgcaaaga aaattgggcc tggtgaatta
      541 atataaataa gtggagcaat aaagataaaa ataattcccg ttgcaataca tccaacaata
      601 cctgcaagaa tatcaattgt tcgttttata aatgcctgtt gagttgtcat aacattgata
      661 cttgttgtaa gtactgtata ttctcctata cgaccaatag attgttttcc caaagcacta
      721 tctgatactt ttgcaagatt caaatgtaca gtaaggccca tttcagagca tcgttcaata
      781 aattctttca tacacgaagt acttttattc aaaataataa agacttcatc tacccatccc
      841 tgacataagt aatccgctgc atttttggaa tcagaaacaa ctgtcactcc ctcaacatct
      901 tcaccaatca tgcttttatc tatgataatg acaccattta ttttatacat tttatagtta
      961 tgctctttca cattacgaat aactacttca gcaatactac tagatgtaac tataataagt
     1021 gatttgttcc cctcattttt cattttatgt ttcaagtaat gtttccataa gaggcgagtt
     1081 atatatgtaa ttacaacgta taaacctccc atcaagaaaa gaacgactct agaataatca
     1141 tttccaccct gaacactaat gagatacaaa cttcctaaca gcaccaacat aatcgcttgc
     1201 tttacagaag aagcaaattc tatatagtac cctcttttta atacacctct atatgcttca
     1261 aacatgaaaa taactaaaag atctgccagt tcaatgaaga ctgccatgtt ttgatacatt
     1321 ggaatcacat aaggattcca atctacctgg cgtaagacat acgatagata aaaagcaagc
     1381 tggatacaca ataaatctaa aataataaaa tcaatatgtt taacccatcc ccttgaactt
     1441 ttcttgtaca ttccttttta ctcccctctt tttaataaaa taaattgtat tacttgtttt
     1501 ctctcctaaa tatctttctg aaggattatt gactcgacaa attggcttgt ggatattctt
     1561 atatatagaa catccgtaag ccaacttaaa actccttaac aaaatttttt tactcatatt
     1621 gtagatcaaa acaagtatcc aagaaatcat attttatttc ttttttctgc ttactcaaca
     1681 acagctaaaa gaaaaatcat cataaacaaa ttcaactaag ccaacgatcc ccttgtgaaa
     1741 tcaagttttt tcttgattta cgaataaaga gctaaaataa accaaacttc ttctttttta
     1801 cctcttcata gactggataa ctgatttcat ccccgttgat cagatccttt gcgacttgtt
     1861 tcatctgtac tactttttcc ttaccaaagt cctttgcaat ctgctcatag cactctttta
     1921 agtaaaaggt gcgcttagag accccatggg catcagaagc agccatctgc acgagattat
     1981 gtttgaccat cgttttggct gttttctgga tgtctttgcc aaatacccca acataacttg
     2041 gcgcagtgag ttgggccaaa catcccatat ctaggaacgg aagcaaatga tttgggtctt
     2101 cgcgaaaatg cacgttgcgt tctggatgga cgattactgg tgtctttcct agttctaaca
     2161 actcaaaaaa aagccgttct gcatataacg gcacttccag tgtcggaaat tcgatcagaa
     2221 gatatgtatc atctaaatct gtaaataata tttcatctcg gtggatgtct tcgatgagcg
     2281 ttccggtaat acgtacttct tgtccttcca ataacgtcaa aggcaactgt cttttctcta
     2341 attcggcttg taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc
     2401 cattattgtg gtgtggtgtg cataaaatat gcgtgatgcc ttgctgaatc gctttttcag
     2461 ccattgcaat acttgcttct aagttttctg ccccgtcatc gattcctggt aggatatggc
     2521 aatgtagatc gatcatttct cttgtctcct ctcttcttat tccttaattt ccgtagtaat
     2581 aaccagcatc tttgctatgt tctgctccgt tgtacacaac acctaagact cgtgcttgta
     2641 ccatctccag cagttctttt gctttagtca aggattcttt acgtgacata ttttcacgta
     2701 cgaccaataa agtgccatcc gtttttaaag acatgatctg tgcatctgtt acggctacta
     2761 ctggtggcat atcgaatatc accacgtcaa ataactgtct tgcttcggat aggatctgat
     2821 ccattcttgg cgagcttaac aattctgatg ggttgggtgg cttaggtcca ctaggcaaaa
     2881 tcgacaagtt ctcgactggc gttcgttgga tcacatctgc tacacttcct gatgaactaa
     2941 gtgctgtact taatccactg gcattgttca gctggaatgt cttatagacg acgggtttac
     3001 gtaaatctgc atcgactagt agaactcgtt gacctgattt agcaaaaacg actgcaatgt
     3061 ttgctgcagt ggtggatttc ccttctccag gacctgatga agtcacgaca atcgttttga
     3121 tttgctgacc agctgcggag gcaaactgga tattcgtccg gatcgtacga tattgttcgg
     3181 cgattggtga agatgggttt gttaatgtga tcaaactgac tgcatgagtt tgtgctgctt
     3241 tttgtttctg tgttcgtgcc atttttcttc ctcctagact cttgagcggc ttctgcgtga
     3301 tggggctgtt ttatcgtttg tagcttttgg tcgtgcagga gctgttcctt ttttacggat
     3361 accagcgttc aattctttcg ctgacatttc tggaactgtt cctaagattg ggaatcctaa
     3421 tgtatctgtc acatattttt catctttcac tgtccgatcg agtaattcca ataagaatgc
     3481 cagacctaca cctaccatca ttcctaacat taagccagtc attaaattca atttattatt
     3541 tggcgaaaca ggtgtcgtat tggcaactgc acctgaaatg atcgagattt tatcaacatt
     3601 catcacatct tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga tctgttgagc
     3661 agccactgca ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt ctgcctgatt
     3721 aactgagatg gcatctttga tttgttcagc agtcattttc acattgtctt ctgtttctaa
     3781 acgatctttg acttcattga gcactaaatc actgacgatc atatctttat acgtattgat
     3841 catttgcagg ttcgtattga catcattgac atttgcagtg tctgattgtg gcagtgtcac
     3901 gatcaattgt gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga
     3961 gatggccaag ccagcaaaca ttgagatgat gattattacc aagtgtttgc gtaatgtttc
     4021 aaaaatttct ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc
     4081 tagattcatt tgatctttta attctttttt cacacgatca agttcttgat cactgacttt
     4141 ttgataggag atattatctt gcatgaagcc ttctccttgc agctgatctt gcttaatcgt
     4201 accaaatgcc tcccgataat ctaaagcaat ttttttcatt tcatcaaagt tcatgtctgt
     4261 tttcatattc gactctagag cattcaaaac ggattgatag ttgctgacac cgtttagact
     4321 caatactttt ttcgctacgc cttctacgat ctttctttgg cgcccttgtc taccgtaatc
     4381 accatttggg tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt
     4441 aattttaccg attggaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc
     4501 gataccacca actgcatcaa cgagttcctt tagtcctttc atattgatag agacatagtg
     4561 atcgattggg atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa
     4621 tgcgtaagcg tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc
     4681 tcgaggaatg ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat
     4741 cgtatctgat cttccttgat cgacccgacc taaatctcca gtatcaatcc ccatcaatag
     4801 aacagagaaa gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg
     4861 ttccactgat tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc
     4921 tactcctaca attgctaata ctaatacgat aagaactgct aaaataatca aaaatatttt
     4981 tttccctctt gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt
     5041 gaatcaacgt tttaactgtc aatagcttat attgctatag cccatttgct gagcaacagt
     5101 ttgtgctttt gttgcgtatg cggctcttat tttttcattc cggatctgct ctatttgact
     5161 gagcgccgtc aaatattgat cgtaagtagc ggtagctgtt acttgaccgt ctttcagcat
     5221 cgtatcgaat agctgatcta gttgcgtgac ttgtgcaatt tgggtatcag cgaatcctag
     5281 atattgcata acgactgttt tccaagcact atccttaaaa aagctcatat tttcacgtac
     5341 atgggcgaca tctgcacttg ccaacttttc tttgatgatg acatcatcca cagcttgctg
     5401 ccagtttgat acgtttttag taaataattt attgacagcc gtctggatct tcagttttga
     5461 ttctgcatcc tctaactgcg ttaagactgc tttcttttct tcttggatcg cagcagcttc
     5521 tttaggtaat tctgattcct tgatgtcata atcatctgcc gaaagtttga tagaagatag
     5581 tttatgtgac aaatttgaaa ctgtttcttc tgttatttca ggatttagga aaattttttc
     5641 ttttgtactg tacagcttat ctacttcttt tgataaagag gtcacggtgt ctttttgatt
     5701 ggtgatggca gattctgcgt aattgatccg ttgatgatat tgccgctgat tattgagatg
     5761 gtatccaaac aagcaaatca caatagcgcc tgtgcctacc gccacagtag ccaataactt
     5821 gacgaacggc ttttccatag attcactcct aaatattgat gatagataag taaagatgtg
     5881 attcattcac atcagaatga gcgcttttta ttttaaaagc ataattaaac taatatattc
     5941 atagaaatat ttataaacag gttttcccta tataatcata ccacatacag tttttttgac
     6001 tagggaaatt cctaattta
//
LOCUS       DASJON010000162         6012 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJON010000162 DASJON010000000
VERSION     DASJON010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.165"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1941)
                     /gene="ligA"
                     /locus_tag="U3989_002311"
     CDS             complement(<1..1941)
                     /gene="ligA"
                     /locus_tag="U3989_002311"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN1715740.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAG"
     gene            complement(1958..4192)
                     /gene="pcrA"
                     /locus_tag="U3989_002312"
     CDS             complement(1958..4192)
                     /gene="pcrA"
                     /locus_tag="U3989_002312"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN1715741.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4347..5906
                     /locus_tag="U3989_002313"
     CDS             4347..5906
                     /locus_tag="U3989_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN1715742.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         1504 a         1291 c         1146 g         2071 t
ORIGIN      
        1 gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac cagtcacttt
       61 cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg ttagtttacc
      121 agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt gggcatttcg
      181 aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca attcatggac
      241 ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga tcgtatcaag
      301 cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga aatgttctgc
      361 taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa tcaaccgctc
      421 gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg atttttcttt
      481 tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag caacatctgc
      541 tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat cgatattcat
      601 cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc atttaggatt
      661 gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac aaactggaca
      721 atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca cgacttgcga
      781 aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca agcggatatc
      841 cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg ttcctgctac
      901 tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga ctgtccattc
      961 gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg ctgcccatct
     1021 cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca ctttgatgac
     1081 gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt caatgtatga
     1141 ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga atccaatttt
     1201 ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga aatcagctac
     1261 tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta actgacgcaa
     1321 agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg cttctctgtc
     1381 ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta cttcgatgtt
     1441 cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt cagtgatgtt
     1501 ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat cttcataacg
     1561 caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta cacgtcccac
     1621 tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa cgtcgttcaa
     1681 actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt ttccgcctac
     1741 tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga ttgctactaa
     1801 ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt ctacgtagta
     1861 ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag cagcttcgtc
     1921 aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca attggcgcaa
     1981 aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt tctagatcct
     2041 tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt ttgactttat
     2101 ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga acaaccgctg
     2161 tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt tttttaggcg
     2221 taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca aaacgagaag
     2281 gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt aaatacaacg
     2341 cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc agttcgcttt
     2401 cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc aggaatacaa
     2461 ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg gaagcatctt
     2521 cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc gttagttttt
     2581 cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg aattcttgtg
     2641 tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat gtattttgga
     2701 tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat tctgtcactg
     2761 tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca cctaattgct
     2821 gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca agcagtggcc
     2881 attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg atgcctcgtt
     2941 tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc agcacattca
     3001 aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct ccaaccattt
     3061 tataaggaat atttgctttc aacagagttt cttccattac acgagattgt gcgttcgtcc
     3121 ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct tcttgcatcc
     3181 ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa gtgatcttgt
     3241 ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg ttttcaatga
     3301 cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct agcaaaatta
     3361 ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct gcaccgcgcc
     3421 agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat ctacctgcca
     3481 ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct acatgaatat
     3541 aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat aatcgaatcg
     3601 tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct ttttggtaag
     3661 cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt gcaaaatcct
     3721 gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt cgaggatcat
     3781 attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc agctgttcag
     3841 aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct cttcttaaaa
     3901 tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca gatgcgagaa
     3961 tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta atcgctagga
     4021 tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt gtcagcactc
     4081 tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg tgaagaacag
     4141 cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac attaacaaca
     4201 tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt aaactagcta
     4261 gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg atttcgcgct
     4321 aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag aaattagata
     4381 agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca ggaatcgtcg
     4441 ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt atctatgaaa
     4501 tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc cttgcagcat
     4561 ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg attccacaag
     4621 tagtgggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta tggaaaaagt
     4681 tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa ggaccttcta
     4741 tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt ccatcttcag
     4801 aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca ttcaatgctc
     4861 ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct ccacttgttt
     4921 ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac tttttcggct
     4981 tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat tatggttcct
     5041 tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat gtcttgctgg
     5101 ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg ttcgttcctt
     5161 tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga ggcaatcaaa
     5221 tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt ttgtttgttc
     5281 ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg attttccttc
     5341 cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg gttgatttcg
     5401 ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt tatttcacag
     5461 cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt ggctctctta
     5521 atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat gaccttctcg
     5581 gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact cagaccgcct
     5641 cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt tctttagacg
     5701 gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct atacgcagag
     5761 gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat attttggtta
     5821 tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct aaatcttcta
     5881 tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca acaactattc
     5941 atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg aaaacccagc
     6001 ctcttctttt tt
//
LOCUS       DASJON010000163         6005 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJON010000163 DASJON010000000
VERSION     DASJON010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.166"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..1106)
                     /locus_tag="U3989_002314"
     CDS             complement(324..1106)
                     /locus_tag="U3989_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715743.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1171..2007)
                     /locus_tag="U3989_002315"
     CDS             complement(1171..2007)
                     /locus_tag="U3989_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1715744.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2025..3434)
                     /locus_tag="U3989_002316"
     CDS             complement(2025..3434)
                     /locus_tag="U3989_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1715745.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3448..5271)
                     /locus_tag="U3989_002317"
     CDS             complement(3448..5271)
                     /locus_tag="U3989_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1715746.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1859 a         1291 c          962 g         1893 t
ORIGIN      
        1 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
       61 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      121 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      181 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      241 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      301 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      361 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      421 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      481 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      541 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      601 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      661 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      721 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      781 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      841 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
      901 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
      961 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1021 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1081 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1141 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1201 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1261 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1321 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1381 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1441 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1501 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1561 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1621 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1681 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1741 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1801 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1861 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     1921 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     1981 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2041 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2101 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2161 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2221 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2281 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2341 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2401 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2461 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2521 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2581 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2641 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2701 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2761 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2821 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     2881 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     2941 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3001 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3061 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3121 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3181 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3241 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3301 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3361 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3421 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3481 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3541 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3601 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3661 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3721 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3781 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3841 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     3901 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     3961 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4021 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4081 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4141 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4201 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4261 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4321 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4381 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4441 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4501 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4561 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4621 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4681 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4741 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4801 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4861 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     4921 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     4981 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5041 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5101 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5161 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5221 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5281 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5341 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5401 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5461 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5521 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5581 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5641 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5701 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5761 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5821 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
     5881 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
     5941 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
     6001 atatt
//
LOCUS       DASJON010000164         5744 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJON010000164 DASJON010000000
VERSION     DASJON010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.167"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            872..1840
                     /locus_tag="U3989_002318"
     CDS             872..1840
                     /locus_tag="U3989_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1715747.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1857..2381
                     /locus_tag="U3989_002319"
     CDS             1857..2381
                     /locus_tag="U3989_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1715748.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2757..3038
                     /locus_tag="U3989_002320"
     CDS             2757..3038
                     /locus_tag="U3989_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715749.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3040..3456
                     /locus_tag="U3989_002321"
     CDS             3040..3456
                     /locus_tag="U3989_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715750.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4086..5240
                     /locus_tag="U3989_002322"
     CDS             4086..5240
                     /locus_tag="U3989_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN1715751.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            5234..5656
                     /locus_tag="U3989_002323"
     CDS             5234..5656
                     /locus_tag="U3989_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715752.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT         2205 a          984 c          905 g         1650 t
ORIGIN      
        1 ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata tatgctaata
       61 atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag ctgaagcagt
      121 ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca cacgagcaaa
      181 tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag tatataaata
      241 tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag acaactttag
      301 ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat aagggattta
      361 tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa gagttcctta
      421 tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag agcagagcga
      481 tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa tgcgcgaatc
      541 tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct cttttataat
      601 ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac tctcccaagg
      661 gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc
      721 tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga attagtacat
      781 cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa cgtgtatctc
      841 ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa agtatcatca
      901 tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt tgttcttttc
      961 tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat tcgagcaatt
     1021 aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg atttagaaac
     1081 aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg gtttgcaacg
     1141 tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata ttccttttgc
     1201 tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag aatgggtgcg
     1261 ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa tgtttcgatt
     1321 actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact ttcatgaatt
     1381 acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt tcaatccaat
     1441 acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat acggaaaagg
     1501 acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa aaaaatcaga
     1561 cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag aactagctaa
     1621 ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca aagaacaaaa
     1681 gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa atacccaaaa
     1741 taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag aactaaaaaa
     1801 agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta gattttatga
     1861 cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca gcaattaaca
     1921 aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac aagtttgtga
     1981 ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac gaaaactcaa
     2041 atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc aaccaagttc
     2101 ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat atgcaactac
     2161 aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa caacaactca
     2221 ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg acttatacgg
     2281 aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg atagaaaaac
     2341 aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagcaa tttaaattaa
     2401 taaaaattgc tggttttatt cttctttttg cttttgcgag cagagcaaca tggacccatg
     2461 ttgcgatatt ttggtggcgc cccaatcccc gttttttcgt tgaatcttac tcggactgca
     2521 ctcgttttac agctgatttt tctgaatttt gaaagagcat aaggaaacgt ggtatcactt
     2581 tcttacgaaa ggtgctagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgctat
     2641 cacttttttg ccattgtggc taggctatgt atagtgtttt ggcgaaaaat agctatacaa
     2701 atactatatt tgtatggtat acataaatta tacgctgata cgaaaggaaa atttccatgg
     2761 acattttagt tcgaaatatc aatcccaaaa ttattgcaag aatagataaa cgttgtcagg
     2821 aactcacaac caaaacgggc aaaaaatgga gtcgaaataa atatctaaaa gttctaatcg
     2881 aaagtgattt tgaccgtcaa cttatgaatt acaaaaaaga tcaattcgat ttattactcg
     2941 aaaaatttat tgccattcaa acagaaaata cgaacatact gaatgactat gttcacacaa
     3001 acaaccaact cattgaaaca cttatcggag gaatataaca tgagaaaaac ttctgttcaa
     3061 aaacacgtaa ccctttatcc cagtcacgtt gacgttttag attccatcat cgaaaaaaat
     3121 ccagacgtca acaattattc agaagccatt cgttttttat gtatgagtta tgaacctgat
     3181 accaatacaa ccacaaccag taaccaagca aaactcaatg ccatgggaaa agaaatttct
     3241 atcatgacag aaatcatggc agaatttgct gatttatatc taaaagatac tggtgttctt
     3301 atcggtacaa attctaaagt gtatcaagaa gtgaaagaaa aaattgaaaa tcaactcaat
     3361 ccttcccgac gaaatcaacc caaaaatcgt caaaaagaaa ccaatcaaac ctttacttct
     3421 ggaaattttg acccaacaaa ctttctaaaa agataaaaaa agaaaccgat ccctaaaaaa
     3481 attcctcgat ttctttttag ggatcagtct tccagcaaga tttgcaagtt ggtaccaact
     3541 tgcgaaaaaa aagaaaaatc cgcttatgca acttcttctt tttgtatttt ggggcgctac
     3601 cccaatcccc aagtatcggt tatttaatag cgcggaaatc caaagaatag gacgtaccct
     3661 gaaacaaaaa aagactgctc tcacgagcag cccaaaatga gattatccta ttcatcaaca
     3721 taaaaatatt acaaagtgaa gaactagaga aagacaaaaa aagcatatca aatcctctca
     3781 aaaataacaa gtctttaaaa aatgacagat aaaaatgcac aaagaactaa aaaagagaaa
     3841 tcgcgtaaaa tcaagcatta gaagctagta actagctaga ccaattaaaa atcacaaagt
     3901 gagcgtctct atccattggt agaggggcta cttggcatag tgtacattat gttaactagc
     3961 tcgataaata acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt
     4021 atcgcatatt gtatatttta attatataat tataggtgta taccacttaa atttaacaca
     4081 aagacgtgat tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga
     4141 tgattggaat ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca
     4201 atatcaactc ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac
     4261 actccataac ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc
     4321 ttggttcgcc atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc
     4381 cagtattttc agacgctgat aatacgatta aatatattta taccgtcaaa aaaaatacaa
     4441 atggagaata ttcaggaagt atctcacaat ttttagcaaa agaacttcaa aatctagtag
     4501 atagtaatat aacagataca accttatttt ttcaagataa tagcatttat tattccgata
     4561 atggacaaat taaactactt tggaaatcac ctttaagtac agaaaaaaat ggtgtttctt
     4621 cctttacaga aaaaaatgat cttatatccg taaatataaa aaataaaatt aaaaatttct
     4681 ctaaaaaaca aaattttcct gcggttaaac gtgtaaaaag tgtctcctcc ggagataact
     4741 ttattatgat taattggcgt atagcagaaa cccaaacaag ttttccgtgg tgtgctgctt
     4801 acgtagaagc tgctatctta tgtaacaaaa cagatgtacg tgctacttca gctagagcca
     4861 ttatgcatta tacctatcct aatttaaaca acagtcaaat tgaatcaaaa agtcttaatc
     4921 aagatcaaat aataaaatat gcaaattact gtggtgttta tcctaaaaaa gtagcacgaa
     4981 tactaaacct ttcagaggtc caaacacaac taagaaaagg aaatgccatt tatatgggaa
     5041 ctgctggtta tggaaattat agtaacagcc gtcatgcact tgcattattt gggtgggtca
     5101 atcaaggtgg ttatcaaact tactatgtgt ggaatccttg gtataactac ccaactgtaa
     5161 taagtgcaaa tgcaggacca gttacaattc ctgtacctgg aggtggtttt acttgggaca
     5221 ataccattac aaattggtaa ataaacccat tgctttagtt atgggtctag tgttagttct
     5281 tttcttaact agttgttcaa aaaaacaaga aatacaaaag actgatctga ttccttttct
     5341 aggaacttgg caaactaaag atcattcaga acgtctagta atcactaatt taaatacttc
     5401 aaaaaatata gaacaatata ccatcttagt aaaagagaac aatcataaaa ttcaaatcat
     5461 tgataaaaat ttaaatataa atgaacaagt tttcatttac aaagcaacaa ataatttact
     5521 aacactgcgc tctaataaaa aacaattaga ttacactttt agtttggatt ctccaaaaaa
     5581 gctcgtattt attttgacaa cgaatgatgg gacagaaggg acagctaaac caattgaatt
     5641 ttttaaagct gattgatgaa tagttgtttg atattacaaa acaataattt caccgaaggt
     5701 gaaacaagcg aaactttaca aaaagtggct agcatttgct agcc
//
LOCUS       DASJON010000165         5694 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJON010000165 DASJON010000000
VERSION     DASJON010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.168"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="U3989_002324"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="U3989_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1715753.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="U3989_002325"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="U3989_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1399..2738
                     /locus_tag="U3989_002326"
     CDS             join(1399..1840,1840..2738)
                     /locus_tag="U3989_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN1715754.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            2850..3746
                     /locus_tag="U3989_002327"
     CDS             2850..3746
                     /locus_tag="U3989_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1715755.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            3844..4053
                     /locus_tag="U3989_002328"
     CDS             3844..4053
                     /locus_tag="U3989_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN1715756.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            4071..4343
                     /locus_tag="U3989_002329"
     CDS             4071..4343
                     /locus_tag="U3989_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1715757.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            4345..5208
                     /locus_tag="U3989_002330"
     CDS             4345..5208
                     /locus_tag="U3989_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEN1715758.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         2266 a          872 c          977 g         1579 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atctcttttt
     1321 caaaataaac ataaaagccc tttaatgatg tgcaccccaa aagttagact agaaatctaa
     1381 ttaaaagggg tgcttttttt gcggaaatat acatttgttt ttaagaaaaa agtagtttca
     1441 gactatttaa ataacgaagg cggctacaaa tatcttgcac ataaatatca aataaatcgt
     1501 acactggtta gacattgggt aaggatttat aactatcatg gttgggaagg cttggttgga
     1561 ggtggcaaaa gctacactac aaaatttaaa cttgatgtta tagaatatat ggaaacaaat
     1621 ggtctttcta tccaagaaac tgctaaaaaa tttaatatcg gttcaaatag aactctaagt
     1681 aaatggatag agcaatatga agaaggcggt gcttcttcac ttgagagcca aaaaaggggc
     1741 agaaaaatta gtatgaattc caagctaaac attcctaaaa aacttaaaga tgagtctctt
     1801 gaagaagaag ttattcgttt aagagcagag aacgcatatt taaaaaagtt agaaaccttg
     1861 attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt cagctcaaaa
     1921 aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca acttattatt
     1981 actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa gaaattacag
     2041 cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta gcgttaaaga
     2101 tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa atgggactta
     2161 cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga aaaattgcca
     2221 agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg gtgacagacg
     2281 tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt gatttattta
     2341 acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg attgaagaaa
     2401 tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta cattcagatc
     2461 aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat aacattattc
     2521 aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat ttttttggtg
     2581 tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata tttcaaagta
     2641 aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag ttaaatggac
     2701 tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg ggttcagttc
     2761 ataaagaggg cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat
     2821 cacaaatcac ttgtgatttg tgattcttaa tgatacaata ttactataca aaaaaagaat
     2881 ggggcgtagt tatggagaag gaaaaactaa aaatacttga agaattaaga cgtattttaa
     2941 acaataaaaa tgaagcaatt attatcttga ataattactt taaaggtggt gttggaaagt
     3001 ccaaattatc gactatgttt gcttacttga cagacaaatt gaatttaaaa gttttaatga
     3061 tcgataagga cttacaggca acattgacaa aagacttagc aaaaactttt gaggtagaat
     3121 tgccacgtgt caatttttat gaaggcttga aaaatggaaa cttggcttct tctattgttc
     3181 atttgactga taatttagac ttgatccctg gcacgtttga tttgatgtta ctgccaaaat
     3241 taactcgctc atggactttt gaaaatgaaa gtagattgct tgctactctt ttagcacctt
     3301 taaaaagtga ctatgatctc attattattg atactgtacc aacgccaagc gtttatacaa
     3361 ataatgcaat cgtggcgagt gattacgtta tgatcccttt acaagcagaa gaagaaagta
     3421 caaacaacat tcaaaactat atttcctatt tgattgattt acaagaacaa tttaaccctg
     3481 gactagatat gatcggtttt gttccttatt tagttgatac ggacagcgca acgataaaat
     3541 caaacctgga agaactgtac aagcaacata aagaagataa cttggttttc caaaatatta
     3601 tcaagcgaag taataaagta agtacctggt ctaaaaatgg aattacagaa cacaaaggct
     3661 atgacaaaaa agttttatcc atgtatgaga acgtattttt tgaaatgctt gagcgaatta
     3721 ttcaattaga aaacgaaaaa gaatagaatc acaaatcaca agtgattaat cacaaatcac
     3781 ttgtgatttg tgattgttga tgataaaata agaataagaa gaaatagaaa gaagtgagtg
     3841 attgtgggaa atttaggcgc acaaaaagca aaacgaaatg atacaccaat cagtgcaaaa
     3901 aaagatataa tgggagataa gacggttcgt gttcgtgctg acttacacca tattataaaa
     3961 attgaaacag caaaaaatgg cggaaacgta aaagaagtta tggatcaagc cttagaagaa
     4021 tatatacgga aatatttacc tgataaactt taaaaaggag tgaaaatgaa atggcagtta
     4081 cgtatgaaaa aacatttgaa atagagatca ttaacgaatt atcggcaagc gtttataatc
     4141 gagtattaaa ctatgttttg aaccatgaac tagatactaa aaatactcgt ttactagaag
     4201 tgaatctttt aaatcaatta gaagtggcac aagaagttga tttatttcaa caaccatttg
     4261 aagaattaca agctattcat gagtattggc ggtcaatgaa tcaatattca aaacaaattt
     4321 tgacaaaaga gaaagtggct taacatggcg aatattactg acttcaccga aaagcaattt
     4381 gaagatcgtt tagaaaagaa tgttgaacga ctaactaaaa atagactagc ggttgaatcg
     4441 ccaaccgctt ttttacttgg tgggcaacca ggatcaggta aaactagttt gcgatcggca
     4501 atttccgaag agacacaagg aaatgttgtt atcattgata atgatacgtt caaacaacag
     4561 caccctaatt ttgatgaact agtgaaactt tatgaaaaag acgtagtaaa atacgttacc
     4621 ccttattcta atcgcatgac agaagcgatc ataagccgtt tgagagataa agggtataat
     4681 ttagtgattg aaggtacagg acgaacaaca gacgttccta ttcaaaccgc aacaatgctt
     4741 caagccaaag attatgaaac aaaaatgtat gtcatggcag tacctaaaat caactcatat
     4801 ttaggaacaa ttgaacggta tgaaactatg tatgcagatg atccaatgac agccagggca
     4861 acaccaaaac aagcgcatga tattgttgtc aaaaacttac cgaccaattt agaaaccctt
     4921 cataaaacgg gcttatttag cgatataagg ctttacaata gagaaggagt gaaactctat
     4981 tcaagcttag aaacgccttc cattagtccg aaagagacct tagaaagaga attaaatcgt
     5041 aaagtatcag ggaaagaaat tcaaccgact ttggagagaa tagagcaaaa aatggttcaa
     5101 aatcaacacc aagaaacccc tgaatttaaa gcaattcaac aaaaaatgga aagcttgcag
     5161 ccacctacac caccaatacc caaaacacct aaacttccag ggatttaaac cttaaaaaag
     5221 taaaagagta gttaccaaaa aacggtaact actctttttt tataaaaaca tttcctcatt
     5281 ttttaaaagc tgatcgagaa ataaagcaca caactgtatt ctttcttttt tcagttggta
     5341 cattttgcag tctgtaacgt gccatttact gtttgttacc ttaacatatc cagtcgaaaa
     5401 cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttgtgtct atgttgcgtt
     5461 ctcgtcacag caaacgacac gaacacactg gcgaaaatga accaacaaaa cgaaaaaaga
     5521 aaggagcgta cactgtaatt tatttctagc ttttaaaaca tgaggaaaac agcttcactt
     5581 cctcaaacgc ctaacaaaaa tggttgtcaa aaaactaaaa atcaaaaaaa gaaaagagga
     5641 aaagaaaatg gaaaaaacaa aaaaattaca attagaagat tttacagaaa atgg
//
LOCUS       DASJON010000166         5585 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJON010000166 DASJON010000000
VERSION     DASJON010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.169"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(488..1297)
                     /locus_tag="U3989_002331"
     CDS             complement(488..1297)
                     /locus_tag="U3989_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1715759.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="U3989_002332"
     CDS             complement(1297..2043)
                     /locus_tag="U3989_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715760.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="U3989_002333"
     CDS             complement(2061..2528)
                     /locus_tag="U3989_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715761.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="U3989_002334"
     CDS             complement(2528..2938)
                     /locus_tag="U3989_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1715762.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="U3989_002335"
     CDS             complement(2949..3962)
                     /locus_tag="U3989_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1715763.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="U3989_002336"
     CDS             4179..4913
                     /locus_tag="U3989_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1715764.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..>5585
                     /locus_tag="U3989_002337"
     CDS             4897..>5585
                     /locus_tag="U3989_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1715765.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1790 a         1184 c          920 g         1691 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatc
//
LOCUS       DASJON010000167         5547 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJON010000167 DASJON010000000
VERSION     DASJON010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.170"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="U3989_002338"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="U3989_002338"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN1715766.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFTMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="U3989_002339"
     CDS             complement(1023..2126)
                     /locus_tag="U3989_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1715767.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3172)
                     /locus_tag="U3989_002340"
     CDS             complement(2216..3172)
                     /locus_tag="U3989_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN1715768.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="U3989_002341"
     CDS             3453..5387
                     /locus_tag="U3989_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1715769.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         1607 a         1089 c         1108 g         1743 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaagc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggtaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5521 tcacaacctc tgttacaacc tcttgtt
//
LOCUS       DASJON010000168         5465 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJON010000168 DASJON010000000
VERSION     DASJON010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.171"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..641)
                     /locus_tag="U3989_002342"
     CDS             complement(<1..641)
                     /locus_tag="U3989_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN1715770.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="U3989_002343"
     CDS             complement(643..1407)
                     /locus_tag="U3989_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN1715771.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="U3989_002344"
     CDS             complement(1415..2092)
                     /locus_tag="U3989_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN1715772.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="U3989_002345"
     CDS             complement(2129..3514)
                     /locus_tag="U3989_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1715773.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="U3989_002346"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U3989_002346"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="U3989_002347"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="U3989_002347"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN1715774.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="U3989_002348"
     CDS             4453..5310
                     /locus_tag="U3989_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715775.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
BASE COUNT         1578 a         1141 c          899 g         1847 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccata
//
LOCUS       DASJON010000169         5430 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJON010000169 DASJON010000000
VERSION     DASJON010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.172"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..1939)
                     /locus_tag="U3989_002349"
     CDS             complement(8..1939)
                     /locus_tag="U3989_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1715776.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1957..2871)
                     /gene="pfkB"
                     /locus_tag="U3989_002350"
     CDS             complement(1957..2871)
                     /gene="pfkB"
                     /locus_tag="U3989_002350"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1715777.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2868..3620)
                     /locus_tag="U3989_002351"
     CDS             complement(2868..3620)
                     /locus_tag="U3989_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1715778.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3779..3988)
                     /locus_tag="U3989_002352"
     CDS             complement(3779..3988)
                     /locus_tag="U3989_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715779.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(3999..5327)
                     /locus_tag="U3989_002353"
     CDS             complement(3999..5327)
                     /locus_tag="U3989_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715780.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         1540 a         1163 c          941 g         1786 t
ORIGIN      
        1 tttatttcta tgcttctgct actgcaggag cttttctcaa taaaccataa atcacaccag
       61 atacgactgc tccgatagca ataaacacca aatacatgac cgggtgactc agtaagaata
      121 caacgaaaat cccgccgtga ggagccagta atttgattcc gaaagctccg actaatccgc
      181 cagttaaagc agaaccaata ataaaacttg gaatggcacg aatcgggtca gctgctgcaa
      241 atggaatcga tccttccgtg acaaacgata gtcccatcac gatgttcgtc aaaccagcat
      301 cttgatctgt tttgctgaat ttattcttaa acaaacgtgt agcaacgaaa attgccaatg
      361 gaggtaccat tcctccagcc ataacagaag ccatcacgac acttccgcca gttgcaacag
      421 tcgtagctaa agttgctgta ccaaacacat aagcggcttt gttgattgga ccgcctaaat
      481 ctgccgccat cattccagct aataatgcac caagtaaagc agcattcgaa ccactcatac
      541 ttgtcaagaa accgttcaat gcatcattga ttgctttcat agggacattg accagcaaca
      601 tcaagaaacc agtaatcagt aagccaaaca ctggataaaa gaggatcgtt ttaattccat
      661 ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa tacgatgaca taaccagcta
      721 agaatccacc aatcagtgca cctaagaatc ctgcaccgcc tgtattagct aatgcaccag
      781 cagcaaatcc aacgatcaaa ccagggcggt caccgatact tgaagcaata aatcctgcta
      841 ataccggcag catgaaacca aatgcagctt gtccgatttg gttgaaccag ctagcagctt
      901 gattgtaatt ccctagattt gataattgat catgtggcac gccaataaat tggtcgatca
      961 tgaaagaaag cgcaatagcg attcctccac caataacgaa aggtaacatg tgtgatacgc
     1021 cgttcatcaa atctttgtaa attctttgtc cgatagtacc atccgcattt ccttcttctg
     1081 aactgctttc ttttccatca ccatggaata ctggtgcagt accactaagg gctagattga
     1141 tcaactcttc tggtttacgg ataccatcac tgacaggacg attgatcagt tcttttccgt
     1201 caaagcgatt catctctact tttttgtctg ctgcaacgat tacaccttct gcacgggcga
     1261 tgtcttcagc agtcaatcgg tttttgattc cttctgaacc gttcgtttcg actttgatct
     1321 ccacacccat ttctttcgct tttttcttca aagcatcttc tgccatgtaa gtgtgagcga
     1381 tccctgttgg acaagctgta acggcaacaa cgaattttct ttctggatga gcagaagctt
     1441 ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc tttttgctct tcagctgttt
     1501 ggaacaagtc ttggacttct tctggtgtat ttgcttcttt taattttcct acgaaatctg
     1561 catcaatcag caatcgagat agtgctgcta atgcttgcaa atgtgtgtcg tttgcgcctt
     1621 ctggagctgc gatcatgaaa aacagataag ttggctgtcc gtctaaggct tcataatcta
     1681 ctcctttttg gctttttgcg aataaaactg ttgcttcttt aactgcactg tttttcgcgt
     1741 gaggcatggc gattccatca ccaagacctg tagaagtttg tgcttcgcgg gctagaattc
     1801 cttctttata tgtttcgata tcggaaatcc ggccggcatc aaacattttt tggaccattt
     1861 catcgattgc gcctttttta tcagtcgctt gcaaatccat gatcatcgct tctttgacca
     1921 ttaaatcctt gatattcatg attatcctcc ttgaaactaa tcaatttttg tgatttcaac
     1981 ttctggcagc aactcatcaa tcaattcgcg agctgccaag tcatcagaaa aagctgttgc
     2041 gcttccgcaa gcaactcccc atttaaaggc ttcgattgga tctttcgtac ggctgtaagc
     2101 cccaataaag ccagcaatca ttgaatcccc tgcacctact gaatttttca atgggcgaac
     2161 taatacattc gttttataag ttccttgttc cgtgaagagc aaagcaccgt ctccagccat
     2221 tgagatcagc acattctgtg ccccttcagc tagtaatttt ttcccatacg gaatgatttc
     2281 ttctacagat tggaaggttg tgttgtaaag ctcagctaat tcgtgattgt ttggtttgat
     2341 caacaacggt ttcttaacaa gtgcattttt taggtcatca cctgttgtat caatcacgaa
     2401 atctgcacct ttttctcgaa tgatatcaat cagttcttga taaaatcctt ttcgcaggct
     2461 agctggcgcg cttcctgaca atacgacgat atctccttgt cctactgttc ctaatgcttg
     2521 cttcaactca tcgatttctt catctgaaat agatgggcct aagccattga tctcagtttc
     2581 agtgtctgat tttagtttga tattgattct tgtatcttct tttacggttg tgaacaaaga
     2641 ctggatttgt tctttttcaa gccacttcga aataaattcg ccagtgaacc ctcctaaaaa
     2701 ccctagtgct gtagattgag aatcgatccg tttcaaaata cgggaaacat tgatcccttt
     2761 tcctccaggt aacttgaagt cttccttcat tcggttcaaa tcgcctagtt ttaacccatc
     2821 tacgcgtacg atataatcaa ttgatggatt aagtgttact gtgtaaatca ttgtattgcc
     2881 tccttaattg ttgtcttttc acggaattca ttaacgaatt ctaatggaca acgctcggtt
     2941 atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg acaactggtt gaatttggta
     3001 tgatcaatca atacaaatgt ctcttcggaa tttttcatgg ctaaacgttt aacagctgcc
     3061 tcttctggat ccggagtcgt gaatccttgt tcaagatggg caccgttcat tcccataaat
     3121 gctttattaa aatgaaaatt ttttagttgt tctatcgcat tagatcctaa aacagcatta
     3181 gttgccagct ttatcgttcc acctaaaata atggtagcaa gctccaaatc aactaaattt
     3241 gccgcatgtt gaacggaatt tgtcactact gtgatctgtt ttccttgcag aaaaggaatc
     3301 atttctaacg tagtagagcc agcgtctaaa taaatgacat cttcgtcatt aatggtttca
     3361 gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt tgagtgattt ttcactcata
     3421 ttctgctcaa aattcaataa ttgctctttc ttagctccgc catgaactct ttctagaaac
     3481 ccactattct ccagctcttg taaatcacgt cttatcgttg attcagaagc attcaaaagg
     3541 tttacaagat cttgtgattt cacgattttc tgctgttcaa gcaagtctag gattttttgt
     3601 ctgcgttcct ctgtaagcat tttcacgatc acctcaatac aaataatagc atattttttt
     3661 tctactagca atataaatca gtcaaaaacg ttcataaaaa ttataaatca gtcaaaaaaa
     3721 aataaaaaac cagtcagaaa gactggtttt ttattttttt gtctatcatc aagccgtttt
     3781 atcgtttttt attttcgctt cttgtttttt tcatataaac agaagcaggt tttctttcct
     3841 tatgaagaag ataataataa agtcccatcc cgccaaacat gaccaaaaaa ccgatgactg
     3901 atcgaatagg aaaattagga acaaaaaaga gacagacgga taataatgat gccggaacca
     3961 ttattcgata atagagttgt ttatccattt tcttcacctc atattttttg taaagtaatc
     4021 aattgttcta attctcggta atctaaaaat tgcacacgca gatgctgctc acctttcttg
     4081 acatcgaatt ggatacttcg ccgctttttc tttattcgtt tgaaggtaac atcagagagt
     4141 ctatgtttga tttcctttaa cggtaattct gacagggatt ctgaaggaac atagattctt
     4201 aaggataaaa gatactcttg atatttttca tctgttacct ctacttctag atattcccta
     4261 atataataac aataagcttc gattccatta tccaaagcaa cagtcggatc gataacttta
     4321 tagcacatca tgcgtgatcg attctcgtca acccaaggaa aatacgcctg agcatttagt
     4381 gttccgcgaa cacgcctttc tttataatct ccttgttgga tatcataact ttttgttaca
     4441 aaaaatgaag cattcttttt aggaacaggt tgcatttgtg cattctgata ctggacagat
     4501 tggatcgtat ggtcagtgta aatgtattca acgaatggcg gtttcaataa accgacacca
     4561 ttgtcccaag caagatgatc aaacccaagc tggtccctaa aataggattg aaacatttga
     4621 ttgtttggct tttgccagtt aaaggggtct cgataaagct ggatacatat gacttcgttt
     4681 tctaattcct tcactttttc tacttgttgg atgtcgagaa aaatcggttt agggatgatt
     4741 tgatgataat agcgactata atacatttcc caacacgaag tgaaacataa accttggtaa
     4801 aaaagatcgt atcctggaaa ttggttgcaa tcgactacta cttccccgtt ttgttgtttc
     4861 attttaggta aatgttcgat ttcatccttt ttctcaaaca atagccgttg agagatctgt
     4921 tttgtattgt gatagataaa ctcgctataa gctcgcatat aggcgaacac cccatgagca
     4981 atcatttttt tctgaatgaa gtctcgaatc aaaaattgct tttctttgaa aacagagata
     5041 gacaatacat tttttatcaa taattgatta tcttccaagc gaaaactaca ttcgttctcg
     5101 tcatttccta caactaacca actttcgtct cttaccgatg ctagcattat tttttcttga
     5161 agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag agcggataaa tgttggctgg
     5221 atcaacagac gtgtcaaaat agcccaatcg cttatttcct gttttttttc ttcttcattt
     5281 aagtctgtga gctggatagt catcaataat ttatcttcgc caatcatttt ctcacctctt
     5341 tcaaatcttt ttataattta attataacca atgataaaga aaaactaaaa ctttatgtct
     5401 tagaaaccaa tttggacgat tacatgatag
//
LOCUS       DASJON010000170         5414 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJON010000170 DASJON010000000
VERSION     DASJON010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.173"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1065)
                     /locus_tag="U3989_002354"
     CDS             complement(<1..1065)
                     /locus_tag="U3989_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HEN1715781.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEF"
     gene            complement(1069..1479)
                     /locus_tag="U3989_002355"
     CDS             complement(1069..1479)
                     /locus_tag="U3989_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1715782.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1497..1988)
                     /locus_tag="U3989_002356"
     CDS             complement(1497..1988)
                     /locus_tag="U3989_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715783.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1998..2789)
                     /locus_tag="U3989_002357"
     CDS             complement(1998..2789)
                     /locus_tag="U3989_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1715784.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2782..3555)
                     /locus_tag="U3989_002358"
     CDS             complement(2782..3555)
                     /locus_tag="U3989_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715785.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3711..>5414)
                     /locus_tag="U3989_002359"
     CDS             complement(3711..>5414)
                     /locus_tag="U3989_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1715786.1"
                     /translation="KIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSS
                     IYLGDKLDSGFWIDPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKE
                     CAGSSEYNYLESDEFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEET
                     SRNFLLSKFRKFRMKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPET
                     HCLIVMHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNG
                     LILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFD
                     SFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIA
                     KEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNE
                     LVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIE
                     RIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTI
                     F"
BASE COUNT         1731 a         1084 c          694 g         1905 t
ORIGIN      
        1 aaactccttt gttaacgatg taatagtcaa agttgtttca aatagaaaat aatcttcgat
       61 tttaggaaca attactcttt ctttttttcc aaattctttc tctaacttaa aacttgaaaa
      121 tggagtgatt tctttaaaat tctcaattat tttttcaggc agtttaacac caagagtttt
      181 gtctgtcttt tgaataattt gatgtggaac aagtgcaccg ccccaatccc agttattttg
      241 atcgttctcc ccttcttttg ttgccaccca gccagttaga aaacggccag tttcattagt
      301 tccgactgtt cttgctgcat aataggcttt tccgtcgaaa gcttcgtcta ctggtgattt
      361 ccaatctgaa aataatcctg ctccgactcg atatttagtc tttttatctt cacaatactc
      421 tgaaaaaagt aaataccacg ttccatcaat ttcaaatata tcaggcattt caatcatgtt
      481 aaattcatta ttagcccagt agtctccttt gaaattccaa tcttttaaat cttcggattc
      541 aaaatagacc aatctccccg tcggcttttt cttacttgct ggttttctag ctcctagtat
      601 caaaacgtat tttccatgat cttgatcaaa aattgcaaaa ggatcgcgcc aatcattctt
      661 atcatatcct tcttgaggaa caagttttgc tgcttcgcca tcctttttcc aatgaattaa
      721 atctttagaa cttgcaatca ttaacacttc tgaagttttt ccatccaact tagctttgcg
      781 attatgtcca gtatacaaag ctaaatactc attgattcct tggacaatac ttccagcgta
      841 aatgtattga tcaatgtcat cgttgccacc acgatgaatt acttccccgt attcttcaaa
      901 tttcacaaaa tcagtggttg taactagtga ccacccaaaa ggatcagtta aaggtttagg
      961 atttctcgta tctcgttgat gaaatagata aaaagttcca tctttataaa aaggcatgca
     1021 atccccaaac caaaaatctt taggcttgta aaaaattttt tgcatttatc actcatcctc
     1081 ttcgttagta tgtctttgca tatctgcaag acacgcaatt gtattcatat ttcttagatt
     1141 ttcgatgata tcatcgcttt tttttccttg taatctcaaa ttgacagcct caatcaaaat
     1201 tcctaaattc atgccaccaa gaagttgaaa cccattttga aatacttcac ttcttttagc
     1261 aaattcgttg aaaggtgttc caccaatcaa gtcagcaaat accataactt cttgattttt
     1321 tacttgagaa actatttttt caatgttttc acggaattct tcaatgcctg catctccaag
     1381 tgttaccaca tgacaattta aattatcgcc aaaaaacatt tttaatgtat ctacaaatcc
     1441 aatggcaata tttccatgag atgtaattat aaactccatc cactcatcct ctgttcttaa
     1501 taaataaaat cctttaaaaa ttcttttgaa tctctaggag tttgtatcac tgtcacttca
     1561 actccatcac taattagttc tttaaaagcc tctctctctt cttcagtaat atttatcgtc
     1621 ggacggatgc gttctgttcc attccttctt gataaatttc ctacgtttac acttgttatt
     1681 ggtaaaccgt tatcaatcaa aaatttaaca tcgattggag aaactgcaac aataagaact
     1741 ttttgtcctt catatttacc agaaagaata tttttaactg ctacttccct tgtaattatt
     1801 gaagttctaa cattaggtgg tgcagcgagg cgaactacag ctttttttac gtcatcattg
     1861 gctacttcat tattaataac cataattctg ttagcactca attgatttgt ccattgtgtt
     1921 gctacttgtc catgaatcat tcgatcatca atacgaacat gtataatacc tttcattttt
     1981 tcatccatat tcaacaccta cttcaaaata tttaacgctc ttaacgcaat agaaactact
     2041 aagattaata gaattacata aactggttta acctttcgtc ctaacaaata atacacaatt
     2101 gctactacac atgcaggtaa taaaccaggc ataacttgat caaagatttc atttccggtc
     2161 attgttaagt cgccttgttt aaattcaaag ccaacattca cttttacaac tgtggcaatg
     2221 agtgcaccga ccaccattaa ccctaaaatt gatactgcat cagtaataga ctttaatttt
     2281 gtttcaattt gagttagtag gctcgcaccc gattctttcc cagcaaggta taaaggataa
     2341 gcagtaatcc ttaataaaat tgatgccagt atccacaaaa taattccaac aaaactacct
     2401 tccaatgcca tattagctgc aattgctcca aaaatagtcc atggaataac tacaaataca
     2461 ctatctccaa ttcccgctaa aggacccatt agacctgttt tcagagcact tactgcttcc
     2521 aaagacttat ttttttcttc tttttcaatt ccaacattca taccaataat gtaaggcgct
     2581 gtatacggcg tagtgttaaa aaattgtagg tgagctaaaa cggattgttt cattccttca
     2641 ggatcatctt tatatgtgtc cttgataaaa ggaagcatag aatacgcata acccaaccct
     2701 tccatttttt cataatttaa tgaagttgca ccattgagaa tccaccggaa aattacttgt
     2761 ttgtcgattt ttgtcagttt cttattcatc ttcgtcaaaa cctcctgttg cagctggtgc
     2821 tacttgagta ttatttgatc tatttttata tacgattagt gcgattgcag cgccaagtaa
     2881 cgaaatccct aaaattggta tttggaaata aactgccaaa acgaaaccaa ctaacaaata
     2941 ggcaaagtaa tcttttgtag gtaaatattt aagtagcatt cctatcccaa cagcaggtaa
     3001 tagtccccct gctaccttta aaccaccggt aacccattca ggaatataat tgattgcact
     3061 tcccactaac gttgggccta atatcacagt taacaaaaca ggaattccag atacaaagct
     3121 tgtattaatt gatgccaaat attgcatcca ctctaatttt tgataatctt ctttttgtgc
     3181 gaatttttct gccttttgtt gaaaaaaaat gtttgttgac cactttagaa catctagttg
     3241 tacaattaac attgctaccg gtattgctaa agttattcct atagaagcat cctggttagt
     3301 tgagattgct aaataagtac cgatcaatgc tgctgtttga taatccggta ctgatgcacc
     3361 accaaaacta gtaataccta gtgtcattaa ttgcaaagtc ccaccgatat aaagtccagt
     3421 ttgaatatct cccataataa gtccggtcaa gaaacctgca acgacaggtt gggaggttcc
     3481 gattttgggc ccttccttat cccagttaat gaaaaatcca tacaacacaa ctaaaaatgt
     3541 ttgccaaaac gccattttgt tctctccttt cttcttatac ttttatataa gcaattactg
     3601 tgccaactaa cattatccaa taaagcggga aaatacagta aatattaatt aaatgagcgt
     3661 ctaaatatac tttggacacc ctttcatttt tttagacgct agtatgctat ttagaaaata
     3721 gtctgctcaa aaattgtata gattaactca tactctcctt tgggtatttc aatacagtaa
     3781 atatctttga tttctgaaaa aaaatctttt gatacattta aaaaagtatc aaatagttgc
     3841 gctccctctt tcaaatgttc atttgttatt ccatcaccaa tcataattcg ttcaatcatt
     3901 ccagaaagat gtagaaatag attaattctc agaaaacttg ggattttgac atcgtaatat
     3961 ttttcataca aatatagtac tttttctatc tcattgaata cttttttgga atctaaaaag
     4021 cgaagcttag tactagcgcc ttcaatagta aagagtttaa ctaattcatt taagcagaac
     4081 tcacagctat ccgagtccat atacttattt aatttttcta aggtttgatt cccattaatt
     4141 agattttcta aatttaaaca atcgattcct tgaatgtaaa attcagtggt tgaaagaaca
     4201 caaattgtat tcttaaaaat catagtatct ttttcctttg ctattttctt taatgcgtta
     4261 aaatcaaaaa tcaaaatctc cacttgatta tcgagatacc gttttaaaat atccttaact
     4321 ttttcagaaa tcccctcacc agagagacaa gatactatta catttttctt ttgtgataat
     4381 cctttataat actgaacttt aacttgaaac gaatcaaaat ccatctggta aaaatgacta
     4441 attggctttt tttggcaaac ttgaatccca cattcaatcg ctaaagctgt agaaacattg
     4501 tttaaaatta ccaaatcacc tattacattg gattcaattt tttcatacat ttgttccaat
     4561 gatcccatat caactagcag aatgagtcca ttcgtcgtat ctatactttt acaaaaattg
     4621 ttgattaggt taattaagtc actagtctca ccttcaattg gcatatcgaa agcctcataa
     4681 acatatgtat tcagcaactt atttgtttcc cttgcgatcg cagaagctgt tattttaccg
     4741 tgcataacta tcagacaatg ggtttctggt attttcacat agaacttctt tacaagaaaa
     4801 agtatccatt tcaacaaaag ttcttgttta ctttcttcga cttctaaact actccaaatc
     4861 tcatttgcca ataatttttg tttatcattt ttcatacgaa attttctgaa cttagacagc
     4921 aaaaaatttc ttgatgtttc ttcaattaca tcaataaaca ttataaatag actgatatcc
     4981 gccaagaatt catctaataa acatagacct gtcgaatcaa taattggtga tattttatta
     5041 acaacatagt ttcttaatgc tataaattcg tcactttcta aataattata ctcagaagaa
     5101 cctgcacact ctttaagtat tctatctctt aattgactca tgctttcttc aattgaaatg
     5161 tgattttgaa aaagttcaat caaatcaatt tcagaaagtg atttaagaaa catttttttt
     5221 aaatctatac taatttctgg atctatccaa aaaccgctgt ctagtttgtc ccctaaataa
     5281 attgaagact ttctcttatt tcctaaataa ccttctgcac aaagaagctt gatttcattc
     5341 ctcaattctc caacatttcc ttcaaatgtt ttcgataaaa gatcagtaaa aagttcaatc
     5401 gaacaaaata tttt
//
LOCUS       DASJON010000171         5386 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJON010000171 DASJON010000000
VERSION     DASJON010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.174"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(48..506)
                     /locus_tag="U3989_002360"
     CDS             complement(48..506)
                     /locus_tag="U3989_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1715787.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(519..1850)
                     /locus_tag="U3989_002361"
     CDS             complement(519..1850)
                     /locus_tag="U3989_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN1715788.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1861..3303)
                     /locus_tag="U3989_002362"
     CDS             complement(1861..3303)
                     /locus_tag="U3989_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1715789.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3290..4054)
                     /locus_tag="U3989_002363"
     CDS             complement(3290..4054)
                     /locus_tag="U3989_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN1715790.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4158..5033)
                     /locus_tag="U3989_002364"
     CDS             complement(4158..5033)
                     /locus_tag="U3989_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1715791.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5288..5372)
                     /locus_tag="U3989_002365"
     tRNA            complement(5288..5372)
                     /locus_tag="U3989_002365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5336..5338),aa:Leu,seq:caa)
BASE COUNT         1639 a         1140 c          793 g         1814 t
ORIGIN      
        1 taaattcacc cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa
       61 caactatctt taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct
      121 ggagaaacta caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata
      181 atagctaaca gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga
      241 cttccttcga gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca
      301 ctttcgatcg ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc
      361 gcatggatat atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa
      421 aagacctcgt cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga
      481 atgtttcttg ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat
      541 tttttgttta ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg
      601 gtttgattca atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct
      661 actagtaact tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt
      721 ggttttctta gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca
      781 actgcatctg accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga
      841 cgacgatcat cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca
      901 aaattaaagt tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg
      961 gtatttttct ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc
     1021 tcaacgacca tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga
     1081 aaatttttcg tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata
     1141 ttaaaatcgg cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt
     1201 tctcgttgca attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa
     1261 tacgtaacga tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt
     1321 gaaatagtca tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct
     1381 tcagaaacga tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct
     1441 tgtatatgat cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct
     1501 ccgagtccac acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt
     1561 tctcgcatct ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa
     1621 gcaagctttg gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc
     1681 ataaatttaa caatcaagta catatccata tttctttctt catcaatatc atataaacgt
     1741 atttcagata atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta
     1801 tacgtactcc cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt
     1861 ctattcttct aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat
     1921 aatttgataa tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg
     1981 ctgttcctcg accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata
     2041 gacatccagg atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc
     2101 ataattttta cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc
     2161 tgccttagct tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt
     2221 aacgtaatac aaagcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc
     2281 gattcgaaaa ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat
     2341 attgaataag taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa
     2401 cataggagaa agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga
     2461 agttcctgca ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc
     2521 tttatagata gctagacata tagctggtat accaaacatc attactacaa aaaaaactga
     2581 taaaaacagt ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac
     2641 ttccttgcta ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg
     2701 gtgcagtcca aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc
     2761 tcccatatca acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat
     2821 aaagcccaaa aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt
     2881 ttcgcccgca aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt
     2941 ccatgccgct actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc
     3001 cataatttct aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat
     3061 tcctttgtcc ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa
     3121 gtttccaaaa acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc
     3181 tgataaacgc atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct
     3241 tccaagcttc tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct
     3301 catatgttac taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa
     3361 ttaaaaattc gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct
     3421 gatccaaata cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga
     3481 gtaaacttgc caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt
     3541 ctcctacgac atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt
     3601 caaagttgct ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat
     3661 tttcatccaa agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat
     3721 gataaatttt ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa
     3781 atggatgctg ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag
     3841 ttttgaggag tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca
     3901 tttcctttct tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg
     3961 atgtcaaaat tcctcgctga taagcatcca taatcccata attcacacca tggctatagc
     4021 caaaatcatc cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa
     4081 aataggcgca accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag
     4141 atagttacgc ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca
     4201 ttttcttgaa tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta
     4261 caatacgctt tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt
     4321 tcttcctgat aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa
     4381 gtcagtgatt ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg
     4441 gtcgtcattt ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta
     4501 atgatatgca tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga
     4561 ttgattcgct cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt
     4621 tctggcaaca taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa
     4681 atatgatctg tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt
     4741 atcagttcaa tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc
     4801 tcactgaatc gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca
     4861 acatcaaatg gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt
     4921 tctattccat cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc
     4981 gttaattcac ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt
     5041 tttgtctcca aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt
     5101 cgtgcctaat tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa
     5161 aagcgttatc aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg
     5221 aggtaattac ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg
     5281 ataattatac cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag
     5341 tccagcgcgt ctgccaattc cgccacgccg gcaaatttaa aaataa
//
LOCUS       DASJON010000172         5385 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJON010000172 DASJON010000000
VERSION     DASJON010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.175"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..341
                     /locus_tag="U3989_002366"
     CDS             <1..341
                     /locus_tag="U3989_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEN1715792.1"
                     /translation="MKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRA
                     YEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNR
                     QQRLFGDDNE"
     gene            519..860
                     /locus_tag="U3989_002367"
     CDS             519..860
                     /locus_tag="U3989_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715793.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            853..1524
                     /locus_tag="U3989_002368"
     CDS             853..1524
                     /locus_tag="U3989_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN1715794.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1530..2222
                     /locus_tag="U3989_002369"
     CDS             1530..2222
                     /locus_tag="U3989_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715795.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            2219..2968
                     /locus_tag="U3989_002370"
     CDS             2219..2968
                     /locus_tag="U3989_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715796.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            2980..3249
                     /locus_tag="U3989_002371"
     CDS             2980..3249
                     /locus_tag="U3989_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715797.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            3255..3419
                     /locus_tag="U3989_002372"
     CDS             3255..3419
                     /locus_tag="U3989_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715798.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            3412..3711
                     /locus_tag="U3989_002373"
     CDS             3412..3711
                     /locus_tag="U3989_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715799.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            3711..4013
                     /locus_tag="U3989_002374"
     CDS             3711..4013
                     /locus_tag="U3989_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN1715800.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            4053..4418
                     /locus_tag="U3989_002375"
     CDS             4053..4418
                     /locus_tag="U3989_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715801.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            4908..5048
                     /locus_tag="U3989_002376"
     CDS             4908..5048
                     /locus_tag="U3989_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715802.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            5045..5227
                     /locus_tag="U3989_002377"
     CDS             5045..5227
                     /locus_tag="U3989_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715803.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
BASE COUNT         1991 a          854 c         1157 g         1383 t
ORIGIN      
        1 aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga attagcggac
       61 aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt ctagggggaa
      121 aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat
      181 ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg
      241 ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa attgatcaac
      301 tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc gctaagaata
      361 gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct aagccaacaa
      421 catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag
      481 agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc
      541 tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa acaagtggtc
      601 gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa gatgatttag
      661 gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag cattcgaata
      721 aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa gagtacgaca
      781 gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt gctgaaatgg
      841 aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt
      901 aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg
      961 tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtacaaacac
     1021 tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt
     1081 atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc
     1141 gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa
     1201 atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta
     1261 tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa gaaattaaac
     1321 atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc
     1381 ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa
     1441 actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag
     1501 agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag
     1561 cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag gaagtgaaca
     1621 ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt gaaattcgtt
     1681 ttattgatcc tagacgtttc accgtacagc aacgaaactt catctatgcg ctgataggcg
     1741 atatttttat cgatacaggc atgccaacgg acttctggaa ggaattcttc tactttcgtt
     1801 ttgaaggtgt cacagggcgc aaaataagcc tgaaagacga atcgaataca actgtgagtg
     1861 atgccaatgt cttagcaaat atcatcttag atttcatctt tgaacatcat attcctttca
     1921 aagaaggcta tgagatttta cctgcgaatc aagaatatta cttctacaaa tgcatcacaa
     1981 aaagagtctg ctgcatctgt ggcaaaacag gagctgacat cgatcacttt gacaaagcgc
     2041 taggaagacg aaagcgcaaa gaagttgatc atgcagagta cacatttgca gcactctgca
     2101 gaatccatca cacagagaag cacaaaatag gtgtgattaa tttcaaaaat aaatatcaaa
     2161 tcaaagggat caaattaaac cagaaaacaa tcaaaaagtt aaatattgga ggggaaaaat
     2221 gacagaacat cgaagttatt atgcgattat accagccaac gtaaggtacg acaaaagact
     2281 taaaccaaat actaagttgt tatacggaga gataacggcc ttgtgtaatg aaagaggctt
     2341 ttgttgggca ggcaatgagt actttgcaga tttatatggt gtgaataaag agaccatatc
     2401 gcgatgggta agtgatttga ttaagtttgg atacttgaat cgggaaatca tttacaaaga
     2461 gggtaccaat caaataatca ataggtacct acgaattaat caatacccta ttgacgaaaa
     2521 acgcaatacc cctattgacg aaaaagtcaa agataataat acatctatta ataatacatt
     2581 taataataca aaagaatata taagagagtt accgccttcg aaaaaatcga aggctaagcc
     2641 cgtccgtcat aaatacggag agtataaaaa tgttcttttg tcagatgagc agatggagaa
     2701 actcaaaaca gaattcccta atgattacca agagcgaatc gaacgactgt cagagtattg
     2761 tgaatcatct ggtaagactt ataaaaacta tttggcaact attcgaagtt gggcaaggaa
     2821 agaaaaaagt gaacctaaga acgcaagcag tggatacaag cgcacaggaa gacgagagaa
     2881 gcttcctgaa tgggcaatcg accaagaagc ctatctcaag aaaaaagcgc tagaacgagc
     2941 taatagacaa tcaaaagcac cattctaaga ggtggaaaaa tgaagatcga ttatctagaa
     3001 ctaattaatg aaatagcgaa ttataaaaag ggcgaggaat tagacgtcct gagagacgta
     3061 tatgatcaac tcgaagaagc tggaattgaa ggaattaaga atgatcgttc gagttggagt
     3121 aaactcagat actatttcgc actctatatc gatggaacgc aattaagaaa tttagcatat
     3181 accaaattac tattcattga ttgtgttaaa ggcttgcaaa aacatcttaa tgaacttgag
     3241 caggtgtaaa caagatggac ctaaagacat ttacagcaca gatcgaatta atgcatcaag
     3301 aagctttaag acaaagtgtg tcgtacgaag acaagtggct caacacattt catggcggac
     3361 gtgagagcgc acttgatcaa gtactcaaat tactgaaagg agaatgtcgg gatggataag
     3421 aaagcggcaa tgcagcgaat tatcgaattg acttattcag aagattggca ggaagataaa
     3481 aaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg
     3541 agaacgccga gaaagattgc aatctggcat ggtgatcgaa ttctagtaac gggtaccgct
     3601 aaacagttat ctgaaattac tggtctgagc aaaaatatta tttgggatag agctaggagc
     3661 ttatggattg attcaaaagg aagacagttt aggtatgtgg aggagaaata atggacgaac
     3721 taatcgcgaa agtagagcag tgggcaaaag ataagggatt ggatcaagcg gatttcagca
     3781 agcaaatgtt aaaaacggtc gaagaaatcg gggaagttgc cgcttctcta gctagaaaag
     3841 atgaacatgg tttaagagac ggaattggcg atgtagtagt aaccttgatc atcttggcaa
     3901 tgcaaaatga tatggattta tacgagtgct tggattttgc ttatgacgaa atcaaaggtc
     3961 ggactggaaa gatggtcaat ggtgttttcg ttaagtccag tgacctgaaa taattccatt
     4021 aatggaagaa ataactgaat aggaggatag acatgattga tgcaaaacaa gtgcaaaaac
     4081 agaaagatgg aatgttgatg tttgaagcat atgttctacc atttcttaat caatttgaag
     4141 ttttggaatg ctctgccagc ggagaagaat tggagtatgt tgtaattcgt gaaaccaaag
     4201 aaaacgttca gaagctaaac gaatttcttt gcactattaa ctgttgggat atgatagctc
     4261 caggattttt atgtccagct atgggagaat ttttggaata ttgtcgtctt gaagatgcag
     4321 gcactttgga tttagcttat ctggtataca actacctaaa catcaacaca gatcatttgt
     4381 ggtttggcac tgccgaaaga aaatgggtag tccgctaacc accaatatca atgaaaaaag
     4441 gcggtttcct gaaagtggaa ttgccgccat tgctgtaagg ttctttacgc gactcaaggt
     4501 cacagaaata taaatgcaaa tctcttttga agtgatccaa agctctcgga aaatggaagt
     4561 tttttgtttc aaagaataaa tgagatttgc gtcaagaacc tgagagttat ttccaaaaaa
     4621 gcttccaaag aaccgaatga gctaaggaca tcatgtgtaa acacataata tagctctcct
     4681 ttcacggcta atcataacat gaaaaatatt cgatgtcagt aggaaaaaag gtgaattatg
     4741 aaaagaatat taaattatcc cggcagtaac agattctgca attaaactcc gaagctgatg
     4801 tatcagtatc aactgtagaa tattatgagc gtggagaaag tcaagcagtc acaaatgtaa
     4861 aaatcgataa cgtcaacggt cttatctgtt tagatgaagg cgatagtatg aatgaaattg
     4921 ctgcagacca tgaattatca gaaactgctt taattgatca aatcgaatta gctagacaat
     4981 actttgttcc atatgcagat acctggtcca agcataagaa tgacatcatt tttccagagg
     5041 aacaatgaga gcaactgatc cagtaattat ccttgaggaa gccaaattta tttggactca
     5101 cgaagagata gagcaagcac gcttgctctt ttctcaagga gttaagccga gcaaagtagc
     5161 tgaaataatg ggtcaaaaga ttcttgatgt cggattgctt ttgctccatc tagcagaaaa
     5221 aatttgattt gaggtgaaaa tgatgattca acttgcaggc atccaaacag ggaaaattta
     5281 tttttctgga gaaagcaaaa gcgaagccaa ccaatggcta ttgaaaactt acacgaacaa
     5341 taagaagcta aggaaaaaat atcctgattt gttgctaaag gatga
//
LOCUS       DASJON010000173         5343 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJON010000173 DASJON010000000
VERSION     DASJON010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.176"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..280)
                     /locus_tag="U3989_002378"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="U3989_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="U3989_002379"
     CDS             complement(546..740)
                     /locus_tag="U3989_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715804.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="U3989_002380"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="U3989_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="U3989_002381"
     CDS             2092..3132
                     /locus_tag="U3989_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1715805.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="U3989_002382"
     CDS             3956..4288
                     /locus_tag="U3989_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN1715806.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="U3989_002383"
     CDS             4907..5248
                     /locus_tag="U3989_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN1715807.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1772 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 ttt
//
LOCUS       DASJON010000174         5312 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJON010000174 DASJON010000000
VERSION     DASJON010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.177"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="U3989_002384"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="U3989_002384"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN1715808.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="U3989_002385"
     CDS             complement(1298..2134)
                     /locus_tag="U3989_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715809.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="U3989_002386"
     CDS             complement(2318..3535)
                     /locus_tag="U3989_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1715810.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3863)
                     /locus_tag="U3989_002387"
     CDS             complement(3684..3863)
                     /locus_tag="U3989_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715811.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3918..4001
                     /locus_tag="U3989_002388"
     tRNA            3918..4001
                     /locus_tag="U3989_002388"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..4819)
                     /locus_tag="U3989_002389"
     CDS             complement(4241..4819)
                     /locus_tag="U3989_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715812.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4825..5208)
                     /locus_tag="U3989_002390"
                     /pseudo
     CDS             complement(4825..5208)
                     /locus_tag="U3989_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac at
//
LOCUS       DASJON010000175         5197 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJON010000175 DASJON010000000
VERSION     DASJON010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.178"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(115..549)
                     /locus_tag="U3989_002391"
     CDS             complement(115..549)
                     /locus_tag="U3989_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715813.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            697..1838
                     /locus_tag="U3989_002392"
                     /pseudo
     CDS             697..1838
                     /locus_tag="U3989_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1964..3043)
                     /locus_tag="U3989_002393"
     CDS             complement(1964..3043)
                     /locus_tag="U3989_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1715814.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3152..3592)
                     /locus_tag="U3989_002394"
     CDS             complement(3152..3592)
                     /locus_tag="U3989_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1715815.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3649..4059)
                     /locus_tag="U3989_002395"
     CDS             complement(3649..4059)
                     /locus_tag="U3989_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1715816.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4240..4674)
                     /locus_tag="U3989_002396"
     CDS             complement(4240..4674)
                     /locus_tag="U3989_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1715817.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4774..>5197)
                     /locus_tag="U3989_002397"
     CDS             complement(4774..>5197)
                     /locus_tag="U3989_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1715818.1"
                     /translation="FHLNTSIGLFFTDAFVVSLNLFVFGIEEFLYAILSIAITSIVMT
                     YIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLEAKGGYNRKSKEVLLIVVTD
                     HEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
BASE COUNT         1887 a          969 c          874 g         1467 t
ORIGIN      
        1 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
       61 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtactatgc
      121 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
      181 ctgactgaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
      241 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
      301 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
      361 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
      421 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
      481 aaaaatatct aattttgtat tgcttttttt cttcaagata aacgttttaa tcgcttcttg
      541 agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt
      601 tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat
      661 ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata
      721 tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag
      781 acagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag
      841 gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc
      901 atcaaagcaa aaattgacta ttcgggagac ttagcaccta tactacaatc agctgttaca
      961 tttgagaatg ttgatcccac tttgtcgatt ggctatgacg agaacacttt gactatccaa
     1021 cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg
     1081 ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatttaac gccgtatagt
     1141 cagcggcaga caattgagca agattcagcc atcgactcag gaggagatct tgttgaagaa
     1201 ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga aaatccagac
     1261 ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac tcaaccagag
     1321 ccagacaata aatcaaaacc tgaaataacc cctggagaaa aaccagatgt aactccagaa
     1381 ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa
     1441 actgaaaatc cagaaaaacc agaggtagat ccagaagaaa aaccagatgt gactccagaa
     1501 ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa
     1561 gaggatggaa aaaaagagag taaaaaatca aatctagcaa ttctaaaaat aaatgaagag
     1621 cagctgaata aaaaatcacg aatatttgat agcgcccaat cagctgaaac cttaaaaagt
     1681 agcaaggata caacctttgc tgtcctgaaa cgaaaaataa acagctacca aaatccggag
     1741 agtcacaaaa caaagttata ctgtggagcg gaatcattct attaagtata gcaacaatgc
     1801 tttctgcaaa aagatttaaa caaaacagat ctctctaaca tttgaataat gtctgtgaac
     1861 gcatttttca aataaaaaga aatatccaga aaatgacccc aaaataccag attgaaatct
     1921 gatatttggg gtcgttcata ctttatcttt cttcatattt attttaaggg atatctgttt
     1981 cttttggaag atggttcggg atctttttca ctttcaatac atctaaaaag aaggtaaaga
     2041 tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca agtaagacca
     2101 caaatgtata aaaaatcggc agttctgttt tgctggacat caacttcgga ttcagcacat
     2161 aagactcaat tagatggaca atcaaaatcg taatcaggat ataaacaaca tctcggatgc
     2221 ctccttggga ataagctatg aaagatagag gaatacaaga aatgataaca cctgctactg
     2281 ggatcagact taaaatgaaa atcatgatcg ctaaactcag cagctgatga aagccaaata
     2341 cagccaaggc gattgtagtg agcaacgtat ttaaaagagc gacaacaaat tgagcttcta
     2401 ataccaaacc aaatgtattg acaaacttat cggcaaaata gtaaatatct tgaaagaacc
     2461 aagaaaattc actttttaaa aacaatcttg agaatacaat tgtttttttc ttttcaatca
     2521 taaaaaagaa acttaagatg aatgacatag caaacgccac tgccattttt cctatatcct
     2581 gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct
     2641 caatataagt atggatatat tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa
     2701 aatcaataac ggactggatc atatcaaatg tttgatgaat aagcatcggg atatactttg
     2761 tgaatgccag atagataagg taaacgatca gtgtataaat catgatagct aaaacacctg
     2821 tagggattcg gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa taagtaaaga
     2881 taaatgtgag caaaatggtt gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa
     2941 ataataaaac agtgagacgg cgtatttttt cattcgccaa aaaacgctga taaacggtca
     3001 aatcgacaac ctccaaataa caaaaaataa acacgttatt cattatatga tggaatatga
     3061 aaataagaaa gagaaagagc aaaaagaaaa accgctaaat gacgttatct tatgagttgg
     3121 ttttcctcat aaaatcgttc atctagcagt ttcagccctt tttctggtct tgatgaaaat
     3181 tatctagcat ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa
     3241 gataagactt gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag
     3301 gagtcagcgt aacatttacg attctttcat cacccgtttc acgctgacga ttaatataat
     3361 ttttttctgc taactttttt agtaagggag tcagcgtacc agaatccaga cacaaatcat
     3421 ttcctagctc tttcactgaa gtctttttct tttcccataa aactaacatc acaagatact
     3481 gggtataggt aagattcagc ggcttgagga gggaagtgta gtgtctaatg atttccttag
     3541 aggcggcata taaaggaaaa catacttgat taactaaata tagatcttcc atttttccac
     3601 ctccgctgtt ttactatagt gtatcatagc ggatttacac aaaaaagtct attcactaac
     3661 tgctttcaga gtaacttcta tatttccagc tgtagctttt gaatacggac acactttatg
     3721 cgctgcctct aataattctt gcgtttcttc cagagaaaga ccttcgatag atccttcaat
     3781 ctctacacct aagacaaagc tcgtttcact ttctgaataa agggaaactt gtgcactgac
     3841 agtagaagcc ccagagatat ttttttgctg tttgatcaat tctaatgcac tgttaaaaca
     3901 tgcactgaaa ccagctgcaa acaattgttc agggttggtt gcattttcga cgcgtttacc
     3961 aggagctgca atattcaatt cgaatgtacg atcgggatta tgaacttctc cagagcgtcc
     4021 gcctgtgttg atcattttcg tagaatacat ttttttcata aaattaccac ctttaattat
     4081 attttataca attgaatttt acacaatata attgtggaaa gcaaagaaat ggattggcat
     4141 aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca aacatataca
     4201 aaaaaatatc agagacaaaa actaatctca tcataaacat tacatatatt tctcaaaaag
     4261 ttgttttctt tcttcaaaac ttacttcacg atggacctga tgaagcatcc ataaataacc
     4321 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     4381 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     4441 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     4501 gttttcatcc agcatatgga atctcgtgcc atacaaggta aaaatgactt cattattgcc
     4561 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     4621 tagtgctttc aaactatcat ttaatacaaa atctatttcc gttcctttat gcataaagat
     4681 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagt taaagaaaac
     4741 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     4801 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     4861 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     4921 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     4981 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     5041 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
     5101 agcataaaga aactcttcta ttccaaaaac aaacagatta agagaaacaa cgaatgcgtc
     5161 tgtaaaaaat aagccaatcg aagtattcaa gtggaaa
//
LOCUS       DASJON010000176         5189 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJON010000176 DASJON010000000
VERSION     DASJON010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.179"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..748
                     /locus_tag="U3989_002398"
     CDS             137..748
                     /locus_tag="U3989_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1715819.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="U3989_002399"
     CDS             821..1750
                     /locus_tag="U3989_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1715820.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="U3989_002400"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="U3989_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="U3989_002401"
     CDS             complement(2693..3871)
                     /locus_tag="U3989_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1715821.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3969..4067
                     /locus_tag="U3989_002402"
     CDS             3969..4067
                     /locus_tag="U3989_002402"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1715822.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="U3989_002403"
     CDS             4098..4499
                     /locus_tag="U3989_002403"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1715823.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5189)
                     /locus_tag="U3989_002404"
     CDS             complement(4706..>5189)
                     /locus_tag="U3989_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1715824.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DASJON010000177         5176 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJON010000177 DASJON010000000
VERSION     DASJON010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.180"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..1098)
                     /locus_tag="U3989_002405"
     CDS             complement(136..1098)
                     /locus_tag="U3989_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1715825.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="U3989_002406"
     CDS             complement(1100..2539)
                     /locus_tag="U3989_002406"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN1715826.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="U3989_002407"
     CDS             2724..4670
                     /locus_tag="U3989_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN1715827.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="U3989_002408"
     CDS             4934..>5176
                     /locus_tag="U3989_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1715828.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DASJON010000178         5146 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJON010000178 DASJON010000000
VERSION     DASJON010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.181"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..244)
                     /locus_tag="U3989_002409"
     CDS             complement(<1..244)
                     /locus_tag="U3989_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEN1715829.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAE"
     gene            complement(608..3274)
                     /locus_tag="U3989_002410"
     CDS             complement(608..3274)
                     /locus_tag="U3989_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1715830.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3615..5066
                     /gene="cls"
                     /locus_tag="U3989_002411"
     CDS             3615..5066
                     /gene="cls"
                     /locus_tag="U3989_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1715831.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1493 a         1095 c          908 g         1650 t
ORIGIN      
        1 tttctgcttc tttttttgca ttttctagca ttcctgaaga aaaatcgatc aaatcatagc
       61 gattaatttc ttttgctaag gaaagaaatc tgccagatcc tccagcaacg tctgctgctg
      121 tttcagcagg caggactttc tccgttttca gataattttt tacatcttca gcaatagtta
      181 cttgcgattc ttgctggatt tcataatatt cttcagcaaa ttcatcccat tctttttcat
      241 tcatttgatt tcctctaatt ttttgttata tgttttcact tttcggaata aatcattctc
      301 ctcttttatc tattctatct cttttgatga ataataaaat gattggcttt ccgtctatca
      361 taaataaaaa aagggactgt gaccaaagtc gtgtcaaagt ccctctttcc gaataaacag
      421 tgtccaatca gatgaccctc agtattagct caaaatgctg attttacaac ttcttcctta
      481 aacggtgttc tcctgccaac tcttcggtca caagtcacaa tattcaacaa acatgagaag
      541 ctgtttgttg aaattgcacc ttgtgactca gaattaaacg gcaggctcac aacctcgttg
      601 attcgtttta tttatttcct tgtactcgta caatgatcaa tttagtgatt tccatcaaaa
      661 caattgtcac aagtgcgatt cccatacatg taaagacttc tgtcaatcca aatgctgatg
      721 ggatagagaa gacttctttc agtcctggga tcattgttaa gcagtaaagc gcgctacata
      781 caactacagc tagtaagaca cttctgttag agaagaaccc tgcttggata gctgtttgtg
      841 tgtttgaacg tgctgggaat gtttgcaatg tccgacacca aatcaatgtt gaaaatgaca
      901 tggctacacc gaattcaggt gaatgctgca tgccaagcca ttgagcaaag ataacagcaa
      961 tcccaatcag cacaccacga tacgttacag aaatcaatgt tttgtccgta aagattcctg
     1021 aatttggatc tcttggcggg cggttcatcg tagcaggttc agctttttct gtacctaatg
     1081 caattgctgg cagtgaatca ttgactaaat tgatgaacaa cagttgtaat gctgtaaatg
     1141 gattggacca gtcagcaact aaggcaaaga tgatcgcaat gatcgcacct aaatttcctg
     1201 caaatagata agaaatagct ttcttgatat tatcaaacac attacgtcct acttctactg
     1261 cgctgacaat agaagcaaag ttatcatccg tcaataccat cgctgctgca tcttttgcca
     1321 catctgtacc cgttcccata gcaattccga tgtctgcttg tttcaatgct ggtgcgtcat
     1381 ttacaccatc acctgtcata gctgagatat gacctttatt ttgccaagca cgaacgatac
     1441 gaattttatt ctcaggtgaa acacgtgcat aaacagtgac tttttctaaa acttcgttta
     1501 gttgatcttc tgtcaattca tctagttccg tcccggtcaa agccaggttt ccttcttcaa
     1561 aaatcccgat ttgttttgcg atagcaacag ctgttgtttt gtggtcacca gtaatcatga
     1621 ttgttttgat tcctgcactt cttgcttctg ttaccgcctg agtcacttca gcacgtggag
     1681 gatcgatcat agcaagcaaa ccaacaagga tcaaatcgtc ttcatcttca aatgataaat
     1741 caaaattatc aagtggacgg taagcaaaag ctagtacacg taatgcgcgt gttgaaaaag
     1801 cttcattttg ctcttggaat tttttcatca attcatccgt catcggaacg acttcaccat
     1861 ttagcaatac ttttttactg cgttggaaga cgatatctgg tccgccttta acaaacaagt
     1921 aatttttgtt atcaattgtg tgtccagtag acattaattt tcgatctgag tcaaaaggaa
     1981 gttctgcttg tcttgggtag ttgtttcgga tttcctgata aggctggttt aatttttcac
     2041 taaagtcgat aaacgctact tccgtagggt cgcccaattt agtaccgtct tcactgatcg
     2101 atgcatcatt cgctaaaaca ctgatttcga tcaagcgacg ttcgtcagct gtccattttg
     2161 aagggtcatt tgtgaaatcc ccagtatcgc cattacctaa aaagtagtca acaactgtca
     2221 ttttgttttg tgtcaatgta ccagtcttat ctgtacagat gatacttgtc gaaccaagtg
     2281 tttcaactgc tggaagcttc cgaataatgg catgacgttt tgccatttta ttcgttccca
     2341 aagataacac gattgttacg atagactgta atgcttctgg aatagcagct acggctacgg
     2401 ctacggcaaa catcactgcg ctgacgatat ctgcagtcat atctcctgta ccttctccaa
     2461 gataaatacg aaacagttgg atacctaaaa tcaataaaga caatgccaaa atagccaaac
     2521 ttagtttttt actgaattta tccagacctc gttgtaacgg agtgacttgc tcagttgttg
     2581 attccaaaag gccagcaact tgtccgatct ccgtattatt cccagttgaa gtcactaaga
     2641 atttaccgcg tccgtaagtt acgatcgttc cactgaatac catatttgta cgatctccga
     2701 ttggtaccgt acttgtgata tccgttactt ctttttctgc agaagtcgac tctcctgtca
     2761 gcataccttc atctactttt aatgatcccg catctagtaa acgtccatca gcaggtacat
     2821 aatcgcctgc gtctaacaac acgatatctc ctaccacaag ttcgtttgcc ggaatcgttt
     2881 tttccttacc atcacgaatc actcgagcat cgggcgctga aaggtttttc aacgcttcta
     2941 aagaaccttc tgcttttttt gtttggacaa cactgattat agaattcaac atcaatactg
     3001 caaaaataac taagaattcg atgtagtcac ccatgaccat ctgaatcagt gcgacaatca
     3061 gcaatacgat aaccattgca tctttgaaag tttctaagaa caatttccaa gttggcttct
     3121 tcggcttttc ttttagctgg ttcaagccat ctctttctaa tttttccttc gcttgcgtgg
     3181 tgctcaatcc atcggttgag ctctgcgttt gctgcatcaa cgagtcgatc gtttctttat
     3241 agaccgcttg ttgtgcttgt tccttgtttt ccaaatttca tcccccattt tttgatcttg
     3301 gccctagtcc tgtctgaaaa aaaagagacc taagacaaat acgcatttgt cttaggtctc
     3361 accatttcat acaataccag aaaaccattt ggttaactta ctgttgatat tatcgcaaca
     3421 aacgttgccg gttactcccc taaggagata ttcaactgta ctaatatcct accttattaa
     3481 cctacaaaaa gcaactactt tagcccgatt tacctacatt ttcatgaaag gagtgaaaag
     3541 tatattcacc ttggtaggct tctttcttct atcatgctat actaatgtta ttgtatgtaa
     3601 ggaggaacat ttttgtggta tctagtatta taaccgccct ttatctatta aatgcactta
     3661 ttgctttggt ggctattttg atcaaacccc gagatgtagc agccatttgg gcatggctct
     3721 tagtattgtt tgcccttcct ggggtgggct ttgtattata tttatttttc ggacgtggat
     3781 taacggataa gaaaaaattc tatctccgac aaagtgactt gaaagaatta gaaaactttc
     3841 agtcttttag aggagatacc attgaacatt acgatcctga catgggcgat aaagacaaac
     3901 agcaatttgt tgacttcttc tcttcattaa atcgtatgcc gctgacaaga atgaattctg
     3961 tcactcttct cacagacgga caagagaaat tggattcact gcttcaagat ctaaaaaaag
     4021 ccaaacattc gatccatatc gaatattacg catttgtgac agataatatc ggccagcaag
     4081 tcttacatgt tttagaagaa aaagccgcag aaggcgtgga agttcgaata ttatatgatg
     4141 catttggctc tcatggcaca aaagcaaaag atttcaatcg tctaatcaaa aatggtggac
     4201 atgtccatac atttgttacc tcacaaaggg cattacttcg tttccgattg aattaccatg
     4261 atcaccgaaa aatcgttgtg atcgatggaa agattagtta taccggtggt ttcaatattg
     4321 ccaatcaata tgtaaataca acaaaaaaat tcggctattg gcgcgatacg catatacgga
     4381 ttttcggtgc cgcttcttct ttgctccagc ttcgcttctt aacagactgg aacgtctcgg
     4441 tacctgaaga aaaaaaggtc ggctatcatt tgaattattt ctttaaaaaa gcagatcgag
     4501 atgaatctaa gcttgctgat acatccatcc agcttgtttc aagcggaccg aataacgaaa
     4561 gggaacaaat caagctttca tttatcaaat tgattacttc tgctaaaaaa cgtgtttgga
     4621 tacagacacc ttaccttgtt cctgatgaaa gtgtcattgc tgctttaaaa atcgcaactg
     4681 cctctggtgt agatgtgaaa atcatgattc ccaacaaacc ggatcatcct tttatttatc
     4741 gagcaacaca atattatgct cggcagctga tcaaggaaaa tgtacaaatc cttgtctatg
     4801 agaacggctt cctccatgca aaaacattga taatggatga tgaaatctgc atggtaggtt
     4861 cagcaaatca agatattcgt agctaccgat tgaattttga aacaagtgct gtcatttacg
     4921 atcctgagtt tttagaagaa cttgctactc agttcaaaga agatgagaca cattgttcat
     4981 ccatgacaac tgaaacagtc aaggaaatgt ctaactggct attattcaag caacaaattt
     5041 ctcgattatt ttctccaatc ctataatttt tttattcaag tagagaaaaa tatactaaga
     5101 ttaaagggac tgtgacaaaa atcttgtcac agtcccttct tccgaa
//
LOCUS       DASJON010000179         5103 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJON010000179 DASJON010000000
VERSION     DASJON010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.182"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            148..1104
                     /locus_tag="U3989_002412"
     CDS             148..1104
                     /locus_tag="U3989_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1715832.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            1393..2091
                     /locus_tag="U3989_002413"
     CDS             1393..2091
                     /locus_tag="U3989_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN1715833.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            2241..3557
                     /locus_tag="U3989_002414"
     CDS             2241..3557
                     /locus_tag="U3989_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715834.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            3574..4989
                     /locus_tag="U3989_002415"
     CDS             3574..4989
                     /locus_tag="U3989_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1715835.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
BASE COUNT         1679 a          808 c         1103 g         1513 t
ORIGIN      
        1 ggcaaaaaaa tatacgaaaa aatcattttg attaactcct tttctttaaa atgtagtaaa
       61 ctaaaaatag aaaaaagaaa gcgttttaaa aagatttgat gaggaggaga aaaattgtat
      121 attgcagata gtgcacgtta tgaaaaaatg gaatatcgta aatccggtca ttcaggtttg
      181 aaattaccga ttttatctct cggattatgg caaaattttg gtgattatga tcctatccat
      241 aatcaaagag agattttaag aggcgcattc gacatgggaa ttacccattt cgaccttgcg
      301 aataattatg gaggccctgc tggagctgca gagaaaaact ttgggcgtat ttttagagaa
      361 gattttcaag cctaccgtga tgaactgatt atttctagca aagcaggtta tcatatgtgg
      421 gaagggccat atggggaatg gggctctcgt aaatccatca tttcaagttg tgaccaaagt
      481 ctccaaagaa tggggctaga ttatgttgac atcttctatc atcatcgccc agaccctgat
      541 acacctttgg aagaaacagc agaagctttg atgcaacttg taagacaagg aaaagcatta
      601 tatattggta tatccaacta taatggagaa gatacaaaaa aaatgactga aattttaaaa
      661 agaaaggaag caccgttcat tattcatcag atgcgctaca atatgttttc tcgtgcacta
      721 ctagaagacg atctatctcc agtattagaa gaagaagggc taggggcaat cacgttcagt
      781 ccgcttgctc aagggttgct gacaaatcgt tatctacatg gtattcctga agattctaga
      841 gcacatcgta aggaaatacc attcctttca gaagaacaag ttggatcaac tctggaaaaa
      901 atcaaagcac tgcaaacaat cgccgtatca cgaggacaat cattagcgca aatggccctt
      961 gcatggaatt tgcgacaaaa atctgttaca agtgtactgg tcggagcaag tcggttaagt
     1021 cagttacaag aaagtgttcg aatgatggat aatttagatt tctcaccaga agaagaattg
     1081 agaatcgacc aaattctaga atagaaacga tgtaatcaaa ctttttatcc atatattaaa
     1141 aaaaacaaaa gatgacatca agaatcgaat gaagatcgtc tgttattgaa catcatattt
     1201 tttgtcgttc aaaataaaaa tgatcgaagg ctaggagtga attttatcct agtctttttt
     1261 atggtaaata tcttcttcgt ttgtatgtct atgaacggat taatcaattt tgtatacaaa
     1321 aaatcatcag atgattcaaa cgtgttataa taattacgaa aaaatacatc agatgaaaag
     1381 gaggtatgtg gtttggaaga aaataaaacc ctagtagaag taactactga caagatcctt
     1441 agatatttaa aggaaatgca gttgcaagta ggagataaac tgccgaatga atatgaattg
     1501 tccgattatt tagaggtggg gcgaagcacg gtgcgagaag ctgtgcgggc tctagcttcc
     1561 aggaatatcc ttgaagttcg gcaaggagcg gggacatttg tcagtgataa gcgagggatt
     1621 gccaatgacc cactcggttt ttctttgatt gaagatcagg atcggatgat acgagactta
     1681 tttgaactga gatatttatt ggagcctaag atggcaggtt tggcagcgga atatgcaacg
     1741 aagaatcaaa tcgtccaaat gaaaaaatta acgcaaaaga ttgaaacttc ttttcgagaa
     1801 ggcacacaag aacatgttca attagacatc gattttcata cgatgattgc agaagctagc
     1861 ggaaacgtag ccttgctgca tattatccca atcattaatg aatcaattgc tttgtttaat
     1921 aaaaattatg atatccagca tctgaaaatc gagacgatcg atattcatac agagatcgtt
     1981 caggccatcg aaaggcggaa tgcacttgct gcgtttgatg caatgactgt ccatatggca
     2041 ataaatcgga gagaattaca gaaaataata ggagaaaaag aaactaattg acagttcatc
     2101 tgatgtattt tatgataaat acatcagatg aatttttgtt ttatatttgg taaaaaagag
     2161 gagtaaatag tttctgttca tcgaaatgta agcgctgact aatagacaaa aagagatgac
     2221 gtcaaggaga agaagcagga atgaggggat ctggaatgag ttttatggat aaattagcca
     2281 atgttttagg gaacgtggca acgagaatca ataatttaag gtatatcatg gtgataaaaa
     2341 acgcatttgc tgcattgatt ccggtaatta ttacgggtgc ttttggcaca ttgttttcag
     2401 caatggtctt tgactcggaa aatggcttag cacagataag tttcttgcgt tttttagaag
     2461 aattaaagcc tatcgcatct gccatcagct atgtcacact aagtttcttg accatctatg
     2521 ctgtttttct gatagggatc gaattagcca agctgaataa agtggatggt gtattccctg
     2581 gtatcatcgc agtgatgtct tatttatctg ttaatccaac tgtttatgaa ttagtgaatg
     2641 aaaatcagac tgttatagta gaaaacgtat tggctaaaca atatacagat acgaaagggt
     2701 tatttttggg aatgttggtc tccatccttt ctattgaact ttattgctgg ttgaggaaac
     2761 agaagaaact acagctgaga atgcctgata ccgtgccaac gaatgtttct gctagttttt
     2821 ctgctttatt cccgacgatt ctgactgtat cgggtatcgc ggctgcggga tttatcatca
     2881 aagaacttac aggaatgtac ctttatgata tcatttacaa tgtcgtacaa cgcccattag
     2941 aaggtgtcgt acaagggtta ccgggtattt tgctattgat gtttattgcg caagtctttt
     3001 gggtgattgg tatccatgga aatcaaatgg tgaagccgat acgggaaccg ttgcttttag
     3061 cggcaattgc tgtaaatacg gaagcttttg aagctggaaa agaagtaccg aacattatta
     3121 ctatgccatt ttgggatatg tatatgagta tgggcgggtc tggagtcacg atcggtttac
     3181 tgattgcagt attgttagtt ggaaaacggg aagatatgaa acaaatcacc aagttatccc
     3241 ttgctcctgg gatcttcaac atcaatgaac cagttatttt tggtatgccg atcatgctga
     3301 atccaatttt agctattccg tttatcttaa cgcctcttat cactggaacg attggttatt
     3361 ttgcaaccgc tttaggattt gcggcgaaag cagttgtaat ggtcccttgg ccaatgccgc
     3421 cgatcgtcaa tgcttatctg gcaacagcag gaaatatcgg tgcagttatt acgcaacttg
     3481 tttgtattat tgtttctatt ttgatctatc tgccatttgt aaaagtagca aatcgtgtgg
     3541 aaaatgatgg gatctaaaaa acaggaggaa atcatgaaaa ttactattcc aaaaaatttt
     3601 attcttggtg ctgcatcttc tgcttggcaa acggaaggtt ggaaaggaaa aaaagaagga
     3661 caggattcgt ttatagatag ctggtataaa aatgaacatt ttgtttggca taatgggtat
     3721 ggcccagccg tagcaactaa ttttatggaa caataccagc aggatgtgga tctgatgaaa
     3781 tccatccatc tgacacatta tcggacttct atcaattggg ctcgtttttt tactgattat
     3841 gagaactgta tagtagatga agaatatgct cagcatataa gtgaaatgat cgactctttg
     3901 atcgaagcag acgtagaacc gatgatttgt ttggaacatt atgaagtccc agcttttctt
     3961 ttggaaaaat atgatggctg gtcttcaaga aaagtagttg atttttatgc taagtatgct
     4021 caaattgcct ttgatcgtta cggtgatagg gtaaaaaact ggtttacatt caacgaacca
     4081 atcgttcccc aaacgcgttg ctatttggat gctgttcgct ggccgcatga acaaaacaca
     4141 aaaaagtgga tgatgtggaa ttatcataaa gttttagcaa cggctaaagc agtagagatt
     4201 taccgcagcg gaaattatgg tggaaggatt ggctgtattt taaatcctga aatggtctat
     4261 gctcgatcca attctccaga agaccaaaaa gctgcttata tgtatgattt gttttataat
     4321 cgagtatttt ttgatccgat ggtaaaagga tactacacag aagagttcct ggagttgtgt
     4381 caaaaacaat cgatttattt cgatcctgca acagaggatt tagaaacgat tcagcatcat
     4441 acagtagatt tccttggaat caatcaatat tatcctaaac gcgttcgcgc ccctcgttat
     4501 caatggaatg aagagacacc tttccatcct gaacgatatt atgaagtctt tgatttacct
     4561 gggaagaaaa tgaataattc caggggatgg gaaatctatc cgaaaatcat gtatgatata
     4621 gccatgtatc taaaagaaaa ttatcataat atcccttggc tgataacaga aaacggtatg
     4681 ggaagagaat atgaagaaca gtatatggat gagaaaggaa tcgtgcaaga tgattaccgt
     4741 atcgaattta tcggacagca tttgcattac ttgatcaaag caacagaaga aggcgcaaat
     4801 tgcgaaggct atatgttatg ggcattcact gactgtgtgt caccaatgaa tgcatttaag
     4861 aatcgatatg gattagttcg aatcgaattg aacgatgaac gaagcagaac attgaaaaaa
     4921 tctgcttcgt ggtatcagac tttgattgat aagagagaat tggatttgcc tgacgaaatc
     4981 tataaataaa tagaagtttt ttgtagatgt tcattgtatt tactggttat ttgaaataaa
     5041 agaggttgtg aatccagcgt tttgagctaa tacccagggt cagctgattg aacaccgttt
     5101 att
//
LOCUS       DASJON010000180         5087 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.183, whole genome shotgun sequence.
ACCESSION   DASJON010000180 DASJON010000000
VERSION     DASJON010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.183"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="U3989_002416"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="U3989_002416"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEN1715836.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="U3989_002417"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="U3989_002417"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEN1715837.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="U3989_002418"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="U3989_002418"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEN1715838.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="U3989_002419"
     CDS             complement(4028..5044)
                     /locus_tag="U3989_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1715839.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DASJON010000181         5043 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJON010000181 DASJON010000000
VERSION     DASJON010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.184"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            129..1154
                     /locus_tag="U3989_002420"
     CDS             129..1154
                     /locus_tag="U3989_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN1715840.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            1329..2573
                     /locus_tag="U3989_002421"
     CDS             1329..2573
                     /locus_tag="U3989_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN1715841.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            2726..3673
                     /locus_tag="U3989_002422"
     CDS             2726..3673
                     /locus_tag="U3989_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1715842.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            3807..4436
                     /gene="upp"
                     /locus_tag="U3989_002423"
     CDS             3807..4436
                     /gene="upp"
                     /locus_tag="U3989_002423"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN1715843.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4575..4817)
                     /locus_tag="U3989_002424"
     CDS             complement(4575..4817)
                     /locus_tag="U3989_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715844.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            4938..5010
                     /locus_tag="U3989_002425"
     tRNA            4938..5010
                     /locus_tag="U3989_002425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4971..4973,aa:Thr,seq:cgt)
BASE COUNT         1677 a          908 c         1089 g         1369 t
ORIGIN      
        1 taaacgactt tttggtttat caacaagttt atccactatt gacaaataga ttatccacaa
       61 acagtggaaa acttgaggat aaactcagac aaaataagaa tgtatgaaaa aggggttgtg
      121 gataaactgt ggaaacgaaa cgatatgatg aaacagaatt gcaagaggca gccgaagcac
      181 tgaaagaagg agaacttgtt gcttttccta ccgaaacagt atacggattg ggcgccaacg
      241 ctcttttacc aaacgctgtc aagaaagtgt tttctgtaaa agggagaccg caagataatc
      301 cattaatcgt ccatgtcgcc tcttttgaac aagtaaaaga gtatgtggac aactttcatc
      361 cggcagcaga gaagattgtg aagaacttct ggcctggacc attaactttg atatttaaga
      421 tcaaaaaaga cactttgccc tcagttgtga cgggaggatt atccacagct gcatttcgta
      481 taccggataa caaaaaaaca ctagaagtaa tcgaactttc cggtgttccg cttgtcggtc
      541 caagtgcaaa tacgtcggga aaaccaagtc caacgactgc agatcatgta tatcatgatc
      601 tacaaggaaa aattaccggg attattgatg atggagctac gcgaattggt gtagaatcca
      661 ctgttcttga tttaagtgat ccaacagcca tgcctatgat tttacgacca ggtgcagtaa
      721 cgaaagaaca aatcgaagca gtcatcgaat ctcctgttgc tattgatcag catcttgtaa
      781 aagaaaatga aacaccaaaa gcccctggga tgaaatacaa acactactca cccgatacac
      841 gtgttctgat ggtcagagat ggcgattggt caactgcagt tcaatgggca aaaaataaga
      901 agatccgtgc aggtgtcata gcaagccccg aaattgcgga tcaagtgcgg acagatactg
      961 cagctgttta tatgtataac gacaactcag tagaagcggc ggctaaagga ttgtttgcag
     1021 gtctaagagg attagatgaa ccaacacttg gattagattt gatttttgta caagtgtatc
     1081 cagaaactgg attggggaat gcttacatga atcgcttgaa aaaagcagca ggacaaaatt
     1141 attttgaaaa atgatcaatt ggttattgtc gcattctttt agtatgatac aatgctatca
     1201 agtaaatctt ctatcagtga acgaaaaact aagcgtaact aatcgtaata gaagagatac
     1261 gaatatttgg caatttgttt ttgatgggaa aaataaaatt gtaggaaaat aaaaaggagc
     1321 tgttgtttgt ggtagattac aaaacgtttg accctgatct ttgggctgcg atcgccaaag
     1381 aagaagaaag acaagaacat aatttggagc tgattgcctc tgaaaacttt gtctccgaag
     1441 cagtgatggc tgcacaagga agtatcttga caaataaata cgcagaaggt tatccagggc
     1501 atcgctatta tggtgggtgt gaatttgtcg atatcgttga aaatttagcg attgaccgtg
     1561 caaaagaatt attcggagcg aaatttgcga acgtccagcc tcactccggc tctcaagcca
     1621 acacggctgc ttatctcgct ttggtagaac caggggacac gatcttagga atggatcttt
     1681 ccgcaggcgg acatctaaca catggatcac cagtcaactt cagtggcaaa acctatcact
     1741 ttgttgctta cggtgttgat ccgacaacag aagttattga ttataatgtg gtacgtattt
     1801 tagctagaaa acatcaacca aaattgatcg tagcaggtgc cagtgcttac ggacgaacga
     1861 tcgactttgc gaaattcaga gaaatcgcag atgaagtagg cgcaaaactg atggtagata
     1921 tggcacatat cgcaggatta gtagcagcag gacttcatcc taatccagtt ccttatgcag
     1981 atatcacaac gactaccact cataaaacat tgcgtggccc tcgaggcggc atgattttga
     2041 caaacgatga agcattagca aaaaaaataa atagtgcggt atttcctggt attcaaggcg
     2101 gccctctaga gcatgtgatt gcgggtaaag cagtagcatt caaagaagca ttagatcctg
     2161 catttaaaga atatagtgaa caaatcatcg ccaatgcaaa agcaatggtc aaagttttca
     2221 atcaagcaat tggaaccaga gtcatttcag gggcgacaga taatcatctg atgttgatcg
     2281 atgttcgcga gttaggtata aatggaaaag aagcagagag cattttggac agtgtgaata
     2341 tcactgtaaa taaaaactcg attccttttg aaacactaag tccattcaaa acaagcggta
     2401 ttcgaatcgg cacgccagca atcactactc gcgggttcaa agaagaagat gctgtaaaag
     2461 tggctgaact agttgtcaaa gcattacaag caaaagatga taacgctcaa ctagacgaag
     2521 tcaaaacagg tgtacgcgaa ttaactgaaa aatttccgtt gcataaaaaa taaaaattta
     2581 aattccatta gtaaacagaa aagttcaatg ttgggctttt ctgtttattt tttgggaaaa
     2641 cggattcttt gacaatatcg gtgatgctct ctagtatatg aggtgtaatt ttagtcttca
     2701 cactaaatct cagggaggaa aagtcatgac tgatcgtatc tcgttagaaa aggaagcagt
     2761 agcattcagt gatgcaaatg cgcctcatcc gcgtatctat gaactagcac cggaagaagg
     2821 aagagtttta ctagaagaag tacaagaatc acctgtgaac aaatattccg tcgatattga
     2881 agacttgatg gtggatactg gcgaatgggg aaaactcaat attcgtctcg tacgaccaga
     2941 agggaatatg gataaattac cagttatttt ttatattcat ggagcaggct gggtttttgg
     3001 cagtgctcaa acccatgata aattgatcag agaacttgcg gttcgtacga attccgtcgt
     3061 tgtctttcca gaatatagtc gatcaccaga agcaaaatat ccaacagcaa ttgaacaaaa
     3121 ttatgctgtg ttgcaacagc tgaataaatt agccaagtca aaaaatctgg atctcgagaa
     3181 tctaatcgtt gcaggagatt cagtaggtgg taacatggct acagtcatga cgatcttaac
     3241 aaaagaacga aagggcctcc caatatccaa gcaattgctg tattatccag tcacaaacgc
     3301 tgattttgat acagattcct atcatgaatt cggagaaaac tattttttga caagagaagg
     3361 aatgatgtgg ttttgggacc aatatacgac gaatgaagaa gagcgtgctg aaatcacagc
     3421 ttcgcctttg agagcaaata taaatgaact aaatgatctt ccttcagcaa tgattttaac
     3481 gggagaagcg gatgtattaa gagatgaagg tgaggcttat gcgcgcaaac ttcgtgatgc
     3541 aggggttcct gttacacaag tacgcttcca aggaatcatc catgattttg tcatggtaaa
     3601 tgctatggat cagacaaagg caacgcgtgc agcaatggca ttatctactt cttggatcaa
     3661 gaaaaaagaa taagctttgc ttgtccgcag gaaaacttct tttcaaaaac ggattttctt
     3721 aggaaaaatg gcgagtttga ctagtcaatc acttgatttt gttttacaat taaaaagaaa
     3781 cgacagaaca aaggagagct taaaaaatgg gcaaatttca agtgatcgat catccattga
     3841 ttcaacacaa actaactatt attcgggaca aaaactgcgg aacaaaggtt tttcgtgaag
     3901 tagtcgatga aatcgctatg ttgatggcat atgaagtatc acgggatatg ccattggaag
     3961 acatcgtgat cgaaacaccg atcactgaaa caacacaaaa aacgttgtct ggtaaaaaag
     4021 tagcgattat tccaatcttg cgcgcaggta ttggaatggt tgacggtatc ttggaattga
     4081 ttccagctgc taaagtggga catatcggtc tttatcgaga tgaagaaaca ttggaaccac
     4141 atgaatattt cgtcaaactt ccagaagaca ttgatgcacg tcagttgttt gtagtagatc
     4201 caatgttggc aacaggcgga tcagcaatca tggcaatcga tgcattgaaa gaacgtggcg
     4261 cgtcgaacat taaatttgtt tgtttggtag cagcacctga gggagtaaaa gctttgcaag
     4321 aagcgcatcc tgatattgac atctatacag cttctctaga tgaaaaattg aatgaacatg
     4381 gttatatcgt tcctggacta ggggatgcgg gtgaccgttt gtttggtaca aaataagtaa
     4441 atacgaaaaa gggtgtgaca caacttttgt cacatccttt tttggtcatt tttaacgttt
     4501 gatgcagaac agctttttca cagctttcgt ttgcatataa aaaaaccatc ccaaaaatgc
     4561 agagatggtc aaattcaaat cttttttagt tctttgtagt taacgattac tacaccctta
     4621 ctttgcgtca tcaagttcgc attttctgca gtttcatcaa tctcaacaac agcggaattt
     4681 ttcaattgct ttgaaatcgt tccagtttgc gacttgccgt gaattgtgaa cattacgtga
     4741 gtattgatct tgaatttttc accaatttcc gatggtacga tagttgtatc aaattttcca
     4801 aaagttgcca ttttcataaa acctgccttt cacatcaatt ataccacgtt ttatgaaaaa
     4861 tatcatgaaa ttaaactaat tttccatcgg actctttaca atcagccaaa tagagtatat
     4921 aatttctttt ggataaggcc agcttagctc agttggtaga gcaacgcact cgtaacgcgt
     4981 aggtcgtagg ttcgatccct atagctggca ttaaaagaga agacagatat atcaaggatt
     5041 ttt
//
LOCUS       DASJON010000182         5039 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJON010000182 DASJON010000000
VERSION     DASJON010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.185"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..1210
                     /locus_tag="U3989_002426"
     CDS             92..1210
                     /locus_tag="U3989_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1715845.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1338..2714
                     /locus_tag="U3989_002427"
     CDS             1338..2714
                     /locus_tag="U3989_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1715846.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3115..4695
                     /locus_tag="U3989_002428"
     CDS             3115..4695
                     /locus_tag="U3989_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN1715847.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4893..>5039
                     /locus_tag="U3989_002429"
     CDS             4893..>5039
                     /locus_tag="U3989_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN1715848.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEK"
BASE COUNT         1689 a          817 c         1043 g         1490 t
ORIGIN      
        1 aaagtatggc aagttttcaa aaataaaaaa agaataaaag aatttttaac gaatataata
       61 tatactagta agaaagaaag gaggaaatct attgtttgaa aaacttagac agtctaaact
      121 aatgttttgg tcgcttgaat tattgatcat agctgcattg atcatgattt catcagaaat
      181 aaactttatt ttcgaaccga tcgggacatt tttttccacg ttatttgcac ctgtactgat
      241 tgcaggcttt ctttactatt tattgaatcc tattgtcaag ctgttgatga aagcaaaaat
      301 caaaagaata tgggctgtcg caattgtgct gctcctttta gttgcagcaa ttatttggat
      361 tttacttagt gtcatcccaa gtctagtaca gcaaatttcc tcattggcat ctaatatgcc
      421 tgatttcatc aaacaggtag aaagctggtt aaaagaggta gccgaattgc ctttgttcaa
      481 ggaagtagat atcaataaat attttgagca attagacatt tcttacggga caatcattca
      541 gcaattcctt agtggagtat ccaatagttt aggttcgatc gttggaacga tcgcttcggc
      601 aaccgtagta atcattacag caccattcat cttgttctat atgttgaaag acggagaaaa
      661 actagtgcca aacatccaac agtttttccc agaaaaaaga agaaaccaaa ttgtggagtt
      721 attggggcaa ctgaataaaa cattggctaa ttacattagc ggacaagcaa tcgaatgttt
      781 attcgttggt accttcacat ttttaggata tatggtaatt ggtgtggatt atgctttctt
      841 atttggggtc attgcgggat tgacaaacct aattccatat ttaggacctt atctaggact
      901 agcacctgca tttctagtaa cagtcttcaa tgatccggta aaagcattac tttgttgtgt
      961 ggttgtgcta atcgtccaac agttggatgg gaatatcatt tatcctaatg taattgggaa
     1021 atcactgaaa atccatccat taacgatcat tatcgttctt ctagtagctg gaaatattgc
     1081 cggattgctt ggtatcttct taggtgttcc tttttacgca atctgcaaga cgatcatttc
     1141 ctatataatc aaaatcgtca aagaagacaa gcaaaatgaa aacaagaaga aaattgctac
     1201 gacctcgtag gtagttttag tacacttgtt tttattgcaa agaaaattgc tttgtgatac
     1261 cattttaacg tggctatagt gccacgtttt tttctatcgt aaactaaaaa ttaattttat
     1321 aaggagagaa tgcgattatg aatgctgacc ctgagagtca gtcgctgtta gcgcagattt
     1381 tattattgat cgttttgaca ttgatcaatg cttttttagc agcttccgaa attgctgttg
     1441 tttcgatcaa taagaatcga atcgagcaaa aagctgatga aggagatgtg aaatctcaaa
     1501 agcttttaaa aatattgcag aatccaaata atttcctatc gacaatccaa gtaggaatca
     1561 ctttggtcaa cattttgtct ggtgcttcac tagctaatac tttatcggaa cggttagctc
     1621 cggtattagg tggcggcaca gcggcaaaaa atattgcaaa tatcattgtg ctggctattt
     1681 tgacctatgt atcgattgtt tttggagaat tgtaccctaa acgtattgct ttaaataaat
     1741 cagaagaagt cgcaaagttc acttcagggc tgatacgctt gattggtgta gtagctaagc
     1801 ccttcgtctg gctgctttct gcttccacaa gtttattggc gcgtatcacg ccgatgactt
     1861 ttgatgatga agattcaaaa atgaccagag atgaaatgcg ttatatgctt gaaaacgaag
     1921 gcgtattgaa taatgaagaa ttagaaatgc tccaaggggt tttttctctt gatacgaaag
     1981 ttgcccgaga agtcatggtc ccacgaacgg atgcatttat gattgatatc aacgacactg
     2041 tggaagaaaa tgtgaatgaa gttttatccg aaaactattc aagaatccct gtttataacg
     2101 aagacaaaga taaagtagtc ggaatccttc atacaaagaa tttactaaaa gctgcccata
     2161 aattcggctt tgataattta gatatcaaga aaatcatgca ggagccttta ttcgttcctg
     2221 aaacggtctt tattgatgat ctgttatatg aaatgaaaaa gacgcagaat cagatggcta
     2281 ttttattaga tgaatatggc ggcgtcgttg ggttagcaac actagaagac cttttagaag
     2341 aaatcgtcgg agaaatcgat gatgaatcag atgaagttga gaatctttat gaaaaaatcg
     2401 atgaacacga atatatcatc caaggacgta tgctgattga tgagttcaat gaagcttttg
     2461 acagtgatct gcatatgagt gatgtagata cgatggctgg ttatttgatt acagcattag
     2521 gcatgattcc ggatgaaggc gaaaagttat cgtttgatgt cgataatatt acgctggttt
     2581 ctgaagaaat ggaaggttct cgaatcctta aaatccgtgt catttttcat gatccggaag
     2641 aaacagaagc ggaacctgaa gaagaacgaa gatacttcag aaaagaattc gaagatgacg
     2701 agccaagaag ataattggaa acttggttat gaaagacgtg ttgcttgaat aaacaagcaa
     2761 cacgtctttt cttttagtca gctgacaaaa aaatatttga ctcttatggt ttggtggctt
     2821 tttcattcgc tgaatccatg ctatactgat tcagttgtag tagttgacaa aggttgtgaa
     2881 aaagctatcc tgcattaagt aataagagca gccaagcaaa aacagctttt cgtatttttg
     2941 gcttgggtgg gcttattgct gataggctgt ctctagaacg ccgtttactc ggaaaaagag
     3001 actgctacac ggcttttgtc acagccctat atgaaattgt ctttctttaa gagatcggac
     3061 aatctgtgcc tagagaaaat ataaataatt gaatggaaga aatggagtta ggttatgaat
     3121 aatccaaaat taaagcaaca agtggacaat cgcagaactt ttgcgatcat ttcccatccg
     3181 gatgctggga aaacaacgat tacagaacaa ttactattgt ttggtggggc tatccgtcaa
     3241 gccgggaccg taaaaggcaa aaaaacaggt aattttgcca aatcagactg gatggaaatc
     3301 gagaaacaac gtgggatttc tgttactagt tctgttatgc agtttgatta tcaagggaaa
     3361 cgagtaaata tcttggatac accaggacat gaagattttt ctgaagatac ttatcgtact
     3421 ttgatggccg tggatagtgc ggtcatggta atcgatagtg caaaagggat cgaagcacag
     3481 acaaagaaat tattccaagt tgtaaaaaaa cgtggcatcc cgatatttac ttttatcaac
     3541 aaactagatc gtgatggtcg tgaaccattg gaattgttag aagaattaga agagctatta
     3601 gatatcgaat cttatccgat gaattggcca atcggtatgg gtaaagggct agaaggtctt
     3661 tatgatatct accataatcg tgtagagttt tatcgtccgg aaaattatca ggatgaacga
     3721 ttagtagaat tggatgaaga tggactgctt cccgaaaatc atcctctaac tgaaaattcc
     3781 ttatatgaac aagtcttaga agaagtagaa ctaatcaaag aggctggaga tgcatttaat
     3841 caagaaaaaa ttgctcgtgg cgatcagaca ccagtatttt ttggttctgc attgactaac
     3901 ttcggtgtac agactttttt agagactttc gttgaattgg caccagctcc ttatggacac
     3961 aaaacacaag atgatcagat ggtaagtcct tatgaggaag aattttcagg tttcgtattc
     4021 aagatccaag caaatatgaa tccagcccat cgtgaccgaa tcgcttttgt ccgaatctgt
     4081 tctggaacat tcgaacgagg aatggatgtc tggttggaac gtacaaataa aaaactgaaa
     4141 ttaagcaatg tcacacaatt tatggcagat tcgagagaaa acgtcgaaaa agcagtagca
     4201 ggcgatatca tcggtgtata tgatacaggc aattatcaga ttggggatac attgttcgaa
     4261 ggaaaactaa aagttgctta tgaagaactc ccttcattta ctccagagct gtttatgaaa
     4321 gtgactgcaa aaaatgtcat gaaacaaaaa tccttccaca aaggaatcta tcaattagtt
     4381 caagaaggag cgattcagct ttataaaaca tatctaacgg aagaatatat cattggagct
     4441 gttggtcagc tacaattcga agtattccaa tatcgtatgt taaatgagta taacgccgaa
     4501 gtaatcatgt ctccaatggg acacaaaatc gctcgctgga tcgatccaga agatttggat
     4561 gagaagatga gttcaagtcg caatatcttg gctcgtgacc gtttcgacca accgttgttc
     4621 ttattcgaga accaatttgc agaacgctgg tttgcggata aatatcccaa tgtaaaatta
     4681 aaaagtttga tgtaaagaag tcttgacaca agctgccctg catcaagtaa taagaatagc
     4741 caaacaaaaa cagcctctca tattttgttt agagccgtga cggattttcc gtgacggctc
     4801 tttttactat attaaaatca tagaaatgtc taatcaaaaa aatataaaaa ttattttgcg
     4861 tacatatgat taaagaaagg agagtgtgac acatggaaac agaatggcaa agacgtgaaa
     4921 gaaaaaaaca gaaaaaagaa gagatgttac gattatgtcg attattagat gtaaaagact
     4981 gggacaaaaa cgagcaaatt gcacaaaaag tacgatcaat catccataaa gaggaaaaa
//
LOCUS       DASJON010000183         5028 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJON010000183 DASJON010000000
VERSION     DASJON010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.186"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(151..2058)
                     /locus_tag="U3989_002430"
     CDS             complement(151..2058)
                     /locus_tag="U3989_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN1715849.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="U3989_002431"
     CDS             2188..3063
                     /locus_tag="U3989_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN1715850.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="U3989_002432"
     CDS             3143..4117
                     /locus_tag="U3989_002432"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1715851.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4297..>5028
                     /locus_tag="U3989_002433"
     CDS             4297..>5028
                     /locus_tag="U3989_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1715852.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLA"
BASE COUNT         1534 a          951 c         1053 g         1490 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagca
//
LOCUS       DASJON010000184         4935 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJON010000184 DASJON010000000
VERSION     DASJON010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.187"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..158)
                     /locus_tag="U3989_002434"
     CDS             complement(<1..158)
                     /locus_tag="U3989_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN1715853.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRH"
     gene            316..579
                     /locus_tag="U3989_002435"
     CDS             316..579
                     /locus_tag="U3989_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715854.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            603..791
                     /locus_tag="U3989_002436"
     CDS             603..791
                     /locus_tag="U3989_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715855.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(874..1059)
                     /locus_tag="U3989_002437"
     CDS             complement(874..1059)
                     /locus_tag="U3989_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715856.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1274..1645
                     /locus_tag="U3989_002438"
     CDS             1274..1645
                     /locus_tag="U3989_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1715857.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1795..1867)
                     /locus_tag="U3989_002439"
     tRNA            complement(1795..1867)
                     /locus_tag="U3989_002439"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1832..1834),aa:Gln,seq:ctg)
     gene            complement(1913..2341)
                     /locus_tag="U3989_002440"
     CDS             complement(1913..2341)
                     /locus_tag="U3989_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715858.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2401..3159)
                     /locus_tag="U3989_002441"
     CDS             complement(2401..3159)
                     /locus_tag="U3989_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1715859.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3454..3651
                     /locus_tag="U3989_002442"
     CDS             3454..3651
                     /locus_tag="U3989_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715860.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3757..4239
                     /locus_tag="U3989_002443"
     CDS             3757..4239
                     /locus_tag="U3989_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715861.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4297..4893)
                     /locus_tag="U3989_002444"
     CDS             complement(4297..4893)
                     /locus_tag="U3989_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1715862.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1590 a          773 c          954 g         1618 t
ORIGIN      
        1 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
       61 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
      121 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
      181 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
      241 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
      301 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
      361 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
      421 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
      481 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
      541 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
      601 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
      661 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
      721 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
      781 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
      841 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
      901 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
      961 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     1021 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     1081 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     1141 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     1201 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     1261 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     1321 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     1381 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     1441 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     1501 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     1561 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     1621 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     1681 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     1741 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     1801 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     1861 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     1921 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     1981 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     2041 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     2101 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     2161 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     2221 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     2281 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     2341 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     2401 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     2461 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     2521 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     2581 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     2641 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     2701 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     2761 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     2821 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     2881 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     2941 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     3001 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     3061 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     3121 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     3181 gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat attttaatat
     3241 gtaggaaata attagttttt aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa
     3301 tacaacagct gttttaattg tgaaaacgaa aacagtacgc tataatgcct gtaaggttga
     3361 tttttaactg taaataaaaa tcaatgagtt aaatctccaa agaaatagca gtggtaatgt
     3421 ggagaaaagt tccagcagtt taggaggatt ttgatgacgg aagaaatgat taatttagga
     3481 gaacaatatg catgcaaacc aattggtttt acaaaaacgg tcattggtga agttgtatca
     3541 aaaatgacaa actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta
     3601 ttagatgaaa aagcaagcat ggttgttgca aaatatgata catttgaata aagaaaacaa
     3661 ttggacgtgc ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag tattcttttc
     3721 ctattttctg gagaagaaaa agcgaggcga gttgtcatgg taacattctt tacggttcta
     3781 ttgtttttat tcgttgttgt gatgttttta tttctttggc tggttcgaaa agaaattatt
     3841 tatcggactg tgcgaaatcg atgggtctac ttggtgatac ccttcttagt gtcactagtc
     3901 atttggtata cactcatcag tcatcctaca gcagatgaat tggcaaaagg gatcttgtca
     3961 gcaatgattt ttattagttt tttgttggac agtagaggaa tcacagaaga aggacttgtt
     4021 ttgaatagct ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat tgtattatat
     4081 cagccaaaag gttctaaaat cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg
     4141 atgctgaaat tttctgcatc attagaagaa ttagttccat ttttgtccca acgcttaaac
     4201 gatgaggcag agattgatat tatgattgat ccagaataaa aacagaggtt gcgacaagat
     4261 gtttgtcaca acctctgttt tttatttcaa ataaaattat ccaaatacgc ggaagcgatc
     4321 ggcatcgttc atgtattctt tttcctcagc cggtgtttct ggtgaaccga atactaattg
     4381 cgaacgtagt ttccagtttg aagggatatt ccattctttt gcaactgctt cgtcaataac
     4441 tgggttgtaa tgttgcaagt ttgcgcccaa tcctttatca actaatgcta cccaagtatt
     4501 agctgttgcg attccgtttg attgttctga ccaatctggg aagttgtctg catataattc
     4561 aaattgttct tgtagacctt ttaccacgtc agtatctttg aagaataata ctgtaccata
     4621 accatttttg aaaccagcta atttgttttg tgtatttggg aaagcttctg ctggagtcaa
     4681 tgggcgaagt gcttcttcag tgatttccca aagtttttca tgtgcatctc cgaaaaggat
     4741 aactgcgcgt gttgattgag cattgaaagc agttgggctt tctttaattg cttctttgat
     4801 caaagttgtt aattcttcat tagaaagtga aacattacgt cctagatgat agattgaacg
     4861 gcggtttttc aaagtgtcag taaaagttgt cataataaaa tttctccttt gatttgcgtt
     4921 ttgcattttt ttaat
//
LOCUS       DASJON010000185         4931 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJON010000185 DASJON010000000
VERSION     DASJON010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.188"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..970
                     /locus_tag="U3989_002445"
     CDS             83..970
                     /locus_tag="U3989_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1715863.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="U3989_002446"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="U3989_002446"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN1715864.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="U3989_002447"
     CDS             2272..2718
                     /locus_tag="U3989_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1715865.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="U3989_002448"
     CDS             2991..4343
                     /locus_tag="U3989_002448"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN1715866.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="U3989_002449"
     CDS             4514..4801
                     /locus_tag="U3989_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715867.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DASJON010000186         4892 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJON010000186 DASJON010000000
VERSION     DASJON010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.189"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..649)
                     /locus_tag="U3989_002450"
     CDS             complement(287..649)
                     /locus_tag="U3989_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715868.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="U3989_002451"
     CDS             complement(723..1103)
                     /locus_tag="U3989_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN1715869.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="U3989_002452"
     CDS             1167..2360
                     /locus_tag="U3989_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1715870.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="U3989_002453"
     CDS             complement(2437..3021)
                     /locus_tag="U3989_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1715871.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="U3989_002454"
     CDS             3222..4220
                     /locus_tag="U3989_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1715872.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="U3989_002455"
     CDS             4332..4841
                     /locus_tag="U3989_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN1715873.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          851 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt tt
//
LOCUS       DASJON010000187         4886 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJON010000187 DASJON010000000
VERSION     DASJON010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.190"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            239..1666
                     /locus_tag="U3989_002456"
     CDS             239..1666
                     /locus_tag="U3989_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715874.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1758..2123)
                     /locus_tag="U3989_002457"
     CDS             complement(1758..2123)
                     /locus_tag="U3989_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1715875.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2120..2485)
                     /locus_tag="U3989_002458"
     CDS             complement(2120..2485)
                     /locus_tag="U3989_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1715876.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2677..2925)
                     /locus_tag="U3989_002459"
     CDS             complement(2677..2925)
                     /locus_tag="U3989_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715877.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2924..3052
                     /locus_tag="U3989_002460"
     CDS             2924..3052
                     /locus_tag="U3989_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN1715878.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3109..4803
                     /locus_tag="U3989_002461"
     CDS             3109..4803
                     /locus_tag="U3989_002461"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN1715879.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
BASE COUNT         1617 a          920 c          846 g         1503 t
ORIGIN      
        1 aacaaacaaa taaaatgact gtccttcatt cactaaacat atttttattt ttttatattg
       61 ttaaaaattc gcaatttatt ttttaataga attatctgtt ttccaaattt tataacataa
      121 aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag
      181 aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat
      241 gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta
      301 tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac
      361 tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca
      421 tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat
      481 gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga
      541 tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc
      601 attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg accgctactc
      661 ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg
      721 cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga
      781 atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat
      841 tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt
      901 tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac
      961 tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac tattcaatct
     1021 ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca
     1081 agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt
     1141 caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc
     1201 ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca
     1261 gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta
     1321 tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct
     1381 ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct
     1441 gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat
     1501 ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg
     1561 tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat
     1621 caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa
     1681 aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac
     1741 acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc tttatttaaa
     1801 aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag
     1861 gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt
     1921 atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc gctttgcact
     1981 tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc
     2041 aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga
     2101 aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta
     2161 tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca
     2221 aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt
     2281 ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac
     2341 gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga
     2401 tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa
     2461 attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt
     2521 cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg
     2581 gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg
     2641 aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa
     2701 gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc
     2761 ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc
     2821 ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga
     2881 actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat
     2941 caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac
     3001 aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat
     3061 ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt
     3121 aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa
     3181 gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat
     3241 gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa
     3301 aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat
     3361 ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa
     3421 gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt
     3481 ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg
     3541 actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa
     3601 gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca
     3661 acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg
     3721 atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa
     3781 tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt
     3841 ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact
     3901 gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat
     3961 gaagatacag cagttatttt cactaatggg tccattacag attcagctac tttaggagat
     4021 tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa
     4081 gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca
     4141 agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc
     4201 cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc
     4261 acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca accgcatttt
     4321 acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt
     4381 ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg
     4441 attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa
     4501 attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc
     4561 aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc
     4621 tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta
     4681 cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa
     4741 acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa
     4801 taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa
     4861 cagcttctta tgtttttgat ttggtt
//
LOCUS       DASJON010000188         4860 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJON010000188 DASJON010000000
VERSION     DASJON010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.191"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            517..1650
                     /locus_tag="U3989_002462"
     CDS             517..1650
                     /locus_tag="U3989_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN1715880.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1681..2001
                     /locus_tag="U3989_002463"
     CDS             1681..2001
                     /locus_tag="U3989_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN1715881.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(2030..2494)
                     /locus_tag="U3989_002464"
     CDS             complement(2030..2494)
                     /locus_tag="U3989_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715882.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2626..3879)
                     /locus_tag="U3989_002465"
     CDS             complement(2626..3879)
                     /locus_tag="U3989_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715883.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3908..4225)
                     /locus_tag="U3989_002466"
     CDS             complement(3908..4225)
                     /locus_tag="U3989_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1715884.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4251..4571)
                     /locus_tag="U3989_002467"
     CDS             complement(4251..4571)
                     /locus_tag="U3989_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1715885.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         1552 a          978 c          871 g         1459 t
ORIGIN      
        1 tgaaaaagct gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt
       61 tttgacttgg ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
      121 ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac
      181 gtttttgcat aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt
      241 tatttatgtc tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat
      301 ccaacatcat ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa
      361 acacacctct ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc
      421 gtttcattat caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat
      481 gcagacaatt ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga
      541 aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca
      601 atcatccgta tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt
      661 gaagcagttt tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa
      721 gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt
      781 agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat
      841 ccagatcagt tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt
      901 ggtcaagcgg aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat
      961 aatgatcttc ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg
     1021 agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca
     1081 gtcggtctat tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta
     1141 gatccagtac agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc
     1201 ttagaagaca cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat
     1261 gtcattatcg attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat
     1321 ttagtaagct tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa
     1381 aaatttggaa cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg
     1441 caagaatttt ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta
     1501 atgccaaaaa tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact
     1561 cattcgctgc cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat
     1621 aaaaatataa aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt
     1681 atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa
     1741 atacgaggac gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct
     1801 gtcgatttat taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc
     1861 cccgatcgat atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct
     1921 attctcagaa tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca
     1981 agcaaaaaaa aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg
     2041 tatcactttg atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg
     2101 tccactcgta tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc
     2161 caagttgatg catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac
     2221 gcttttccgg ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga
     2281 agtcgttttc aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat
     2341 accattgagg aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa
     2401 cagcaggaac tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt
     2461 gttctcttaa tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa
     2521 cgtttacaaa aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa
     2581 aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc
     2641 ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga
     2701 aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc
     2761 gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt
     2821 agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa
     2881 catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac
     2941 acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa
     3001 ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc
     3061 aaagaaaggt ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt
     3121 tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg
     3181 taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga
     3241 gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc
     3301 aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc
     3361 tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat
     3421 gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat
     3481 aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga
     3541 gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata
     3601 agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc
     3661 cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg
     3721 aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac
     3781 cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg
     3841 tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta
     3901 ctgtagatta cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa
     3961 aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt
     4021 gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag
     4081 attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag
     4141 caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg
     4201 cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt
     4261 attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata
     4321 ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata
     4381 aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc
     4441 gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt
     4501 tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag catgattgtt
     4561 ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg
     4621 tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt
     4681 tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt
     4741 tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat
     4801 aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga
//
LOCUS       DASJON010000189         4817 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJON010000189 DASJON010000000
VERSION     DASJON010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.192"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..612
                     /locus_tag="U3989_002468"
     CDS             <1..612
                     /locus_tag="U3989_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1715886.1"
                     /translation="VIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLEY
                     ASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSFG
                     NLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFF
                     FVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            609..1328
                     /locus_tag="U3989_002469"
     CDS             609..1328
                     /locus_tag="U3989_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1715887.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1343..2428
                     /locus_tag="U3989_002470"
     CDS             1343..2428
                     /locus_tag="U3989_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1715888.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2564..4096
                     /locus_tag="U3989_002471"
     CDS             2564..4096
                     /locus_tag="U3989_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1715889.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4274..4495
                     /locus_tag="U3989_002472"
     CDS             4274..4495
                     /locus_tag="U3989_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN1715890.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1684 a          513 c          935 g         1685 t
ORIGIN      
        1 gtgatcaaga tattatcatg cgtaatactt gtgtcgatag tattatcctt ttttataaca
       61 atatttatta cattgaacga taatggacaa agctatgctg ataatttcac aattcaaggt
      121 cttttagaat atgcgtcaag agattctgga tatgatggtg ttggtgattt aaatcgaata
      181 actggtatct ctagagtagt agatgaaata tttaataata attttagatt agtcctaatg
      241 ggagtaggca ttggcaatgc agagtttacc aatttttttg tcagtgagtt ttataaatcg
      301 tttggtaatc ttcattatca gtattttcat catatttggt tatttataga agttggaata
      361 ttaggagttt cgatattcct attttttata ataaagacat ttttcaatgc aaaaagatct
      421 tttatgaagg ataaagatgc aagtcttgtt aaagttattg caattatgtc ggtattcttt
      481 tttgtgtata acacaacact taggggtgaa acaacggggc cactgattta tatgatttta
      541 gccatgcctg aagttgtgaa aaggaaagag cattattata gtgaaaaaaa tacaaaagga
      601 gagatattat gattcctaag aagatacatt actgttggtt cgggggaaat ccacttccga
      661 aaaaagatag aaagtgcata gaaagctgga aaaagtattg ccctgattat gagattattg
      721 aatggaatga atcaaattat gatttaaata agaaccgtta tatggccgat gcatataaag
      781 aacgtaaatg gggttttgtt ccagattatg caagatttga tattatttat aatcatggtg
      841 gattttattt agatacagat gttgagctaa ttaagtcgtt agatatactt cgtgaaaatc
      901 atgcctatat gggatttgaa ggggaaatct gggttaatgg cggtattggt tttggtgcag
      961 aagaaggaca ttctgttatt aaaggtttaa gggatattta cgattcatta agtttttatc
     1021 aaaatgatgg tacattgaac ttgaaaccga gtccgtatta tattacagat tatcttgaat
     1081 ctattggatt ggttcgcgat aacagcgaac aaataataaa tggcctgcat atctatccta
     1141 cggattattt tgctgccaag gattatgata ctggaaaaat ttataaaact agtaatacaa
     1201 taagcattca tcaatataat gcatcttgga aaacacctaa acaaaaattt gtttatagat
     1261 taaaatcaac aattggacaa aaaaaattta acaggttact tgcactgaaa aaatttttac
     1321 acttataaag gagaaaagta ctttgaagaa aatttgtttc attagtggtg taatttctcg
     1381 ttctggggga actgaaagag tagggagtat tattgccaat agcttgtctg atttaggtta
     1441 tgatgtcact attttaagct tttggaatag tggagagcct tattttgggt tagataagac
     1501 gattaaggtt gattatatgt taaatcctaa aacggaggga aaattatata gaacttattt
     1561 atatccggtt tggaaattgc acaagtatat atgcaaaaat gaatttgata tagtaattga
     1621 tattgatact ttgttgtctt tgtatactac aagggcaatt aaaggaacaa agtgcaaatt
     1681 gatttcttgg gaacatttta attactggac aatggaaatg ctgaaagaaa agaagagatt
     1741 caaggctaaa aaattgataa aaaaatatgc aaagaggctt gtggttctca cagaagaaga
     1801 tcgtcaaaaa catattgatg aatatagttt atcagaagat tttgttataa ctatgccaaa
     1861 tccgtgtata tctaatatcg agtacgaata tcattatgat aaaaagagat ttcttgcagt
     1921 agggcgatta gtggaagaga aaaattttgc tgacatgata agtgcttgga aaattgtaga
     1981 aaaaaaagta gatgattggg atcttatcat tgtaggaaaa ggtgaattag aaaaagatct
     2041 tcgccagcag ataaaagatt taaatctgca aagagtaact atagcagggc attcagacca
     2101 tgtggaggaa tattataaat ctgcatcttg ttatgtgctt agttctgttt atgaaggatt
     2161 tcctatggtt atattagaag cacagtctta tggattacca gttatttctt atgattgtaa
     2221 aactggtccg agagatttgg tacatcataa ttttaatggg atgttggttg aagataagaa
     2281 tatagaccaa ttagctaaga gcatgataat gtttactaag aatactgatt tagcaatgag
     2341 aatgtcactg aacgcatata ataatgtaca aaaatttaat ttgaaagaaa ttacaaaaca
     2401 atgggttgct ttgattgaaa atgtatagtt ttttgtggtt gtccaaacat gaaaattagc
     2461 gtaattaatc aaagagctag aagaaaccta ttacatttca agattttaga aattatatag
     2521 tattaggtaa caataaaaaa tagaaagttt ggagccatat gatatgataa ttaatcgtaa
     2581 acaaaatgct aaaaggaata taattactgg aacaattttt aagttatatc agattatcat
     2641 tccctttttt atgcgtacac ttatgattta tcagatggga atggagtatg ctggattgaa
     2701 caacctgttc acatctatat ttcaaatgtt aaatttagcg gaattgggaa taggtgctgc
     2761 actaactgta agtatgtata aacctatagc agaagacaat agaaaagaga tttgtgcttt
     2821 actgaacgca tataaaaagt acttttcaat tattggatgg atagtgttta cattaggatt
     2881 gttatgcatt ccttttttga gatttttagt taaaggttcg attcctggaa atttaaatat
     2941 gacagttttg tatttaatgt atttgataaa tactgttttg tcgtattggt tattttcata
     3001 tagaaagagt ctaatatatg cacatcaaag aaatgatgta attaacgtgt ctatgctaat
     3061 tacttttaca ttccaatttg taatgcaggc atatgttctg attttcttaa aaaattacta
     3121 tttgtattta ttggcttcta ttttgggaca aatagttctt aacttaatgt gtgctgttgt
     3181 ttctcaaaag atgtatcctg attatactcc tgttggtgag ttagagaaag aaactattga
     3241 cgatattata aaaaaagtaa aaggattagt tacaaacaag gtaggcggta caatattacg
     3301 atcttcagat tcaattatta tttcatcttt cttaggtctt tcagtacttg cgatttatca
     3361 gaattattac tttattattt ctgccataat gagtatattt gcaataattt atgaatcatg
     3421 tatagcaggt ataggtaaca gtcttattgt agaaagtcaa gaaaaaaatt atatagattt
     3481 ttgtgatatt acaacaatta taggttggtt tctggcaatt agttgtgcat gctttattac
     3541 attatttcag ccgttcatga ttatctgggt gggaagtgat aatcttatga gtatttcatt
     3601 tgttatattt atgatagttc attttttctt atatgaaatt gatcagttaa ttgggttata
     3661 caaagatgct gctggaatat ggtacgcaga caggtttaga ccattaatta cagcaatgat
     3721 aaacattgta ttaaatatca tcctcgttaa ttggttcgga ttatatggag ttttggcggc
     3781 aaccattgtt tcagtattgt tggttgattt accctggtta ttatataatg ttttccaaaa
     3841 tatatttaaa tttgaatcgg caggtaaata tgtttggaag ctcataagtt tgtgtgtaat
     3901 agcttttgtt gcttcttatt taagttattt tgcttgttcg tatgttaata tcggaaattt
     3961 caaagtgata agtttagtga taaatttagt tatatcggtt gtcatatcga ttttagttat
     4021 tcttctgttt aatatccgta ataagaagtt taagtatatt ttgaaagttc ttacgtcggt
     4081 attggtaaaa agatgagaag agacatcaga atgatatata aagtaactac aatttttagg
     4141 ctgccgaata ggcgaacgat gtatagtttt tgttccaacg aagactcttg ttgaaattgg
     4201 agatgatatg aaggagttac aattttgact catgtttatg attgatcggt tttaaaagta
     4261 gtatggagaa gctttgggat catcggagag gatcaaaata ggtaataatg tttttatagg
     4321 aatgaagtct agaatactaa agggtgtaca tataggaaat aatgtagtta taggtgcaaa
     4381 tagtttagta aacaaggacg tatcagacga ttgtgttgtt gcaggaaatc tagcaaaagt
     4441 aattatgtcc ctagatgaat attataaaaa gataaacaat atgaagaatc cgtaaaactt
     4501 gtgaaactat accgagaata atatgggaaa aatattgatg aaaaagcatt atatgagttt
     4561 ttagggctgt ttacagatgg agatgacaat ttgtcgaaga gttgggagca atgcaaaatt
     4621 tagtagggga ttataaacag actgacaagg tatttaaaga acatgcacaa gtatttcata
     4681 atattgaaga atttttgaat actgtgagat aggagttgct acattgagaa aagtatgtat
     4741 ttttccaatg taaaagatta cgagtttgaa aacgacacca taatattatt ttttaagaag
     4801 atattataga gcttagg
//
LOCUS       DASJON010000190         4795 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJON010000190 DASJON010000000
VERSION     DASJON010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.194"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            69..869
                     /locus_tag="U3989_002473"
     CDS             69..869
                     /locus_tag="U3989_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1715891.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            888..2756
                     /locus_tag="U3989_002474"
     CDS             888..2756
                     /locus_tag="U3989_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1715892.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2749..3240
                     /locus_tag="U3989_002475"
     CDS             2749..3240
                     /locus_tag="U3989_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN1715893.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3228..3782
                     /gene="srlA"
                     /locus_tag="U3989_002476"
     CDS             3228..3782
                     /gene="srlA"
                     /locus_tag="U3989_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN1715894.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            3800..4795
                     /locus_tag="U3989_002477"
     CDS             3800..4795
                     /locus_tag="U3989_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN1715895.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1670 a          751 c         1017 g         1357 t
ORIGIN      
        1 catgtcttaa ttttttttag tgagctttta tactggattt gtaagaaaaa agaaggagag
       61 gatcacatat gtcggattgg ttggatattg caggaaaagt agtaattgta actggaggtt
      121 cctcaggaat tggaagaagt atcgtcgaaa atttgttgaa gcagaatgca caagtagcga
      181 attttgatgt aactgaatgc cgtatacagc atgaaaacct actatcttta aaagtcgatg
      241 tgtcttctaa aacggacata gaagaaggga tatataaagt aatgaaacat ttcaagacga
      301 tcgatggtct agtcaataat gcaggaataa atattccttc tcttctaata gatagaaatc
      361 atccaaaaag caagtatgaa ttatcagaac aagtttttga taagatgatc gctgtcaatc
      421 aaaaaagtgt atatttgatg agccaagcag ttggaagaat cttggtgcaa aagggatcag
      481 gagtgatcgt gaatctttct tctgaatcag gattggaagg atcagaagga cagagctgtt
      541 atgctgctac aaaagcagcg atgaacagtt ttactcgttc ttgggcaaaa gagttaggaa
      601 agaggaatgt tcgtgtggta ggtgttgcac cagggatact ggaagaaacc ggtttgagaa
      661 cacaagaata tgaagaagct ctttcttata cgagaggtat aagcgtcgaa cagctgagaa
      721 acggttacag tcgaacaagt acgatccctc taggaaggag tggtaagctg caagaagtgg
      781 cggatctagt ctgctattat ctttcagaaa gatcaagtta tatcactgga gtcacgacta
      841 atatttcagg aggtaaaaca agaggataaa ggataggggg gacgcacgtg acgctagtaa
      901 acagatggta ccaattattg aaattactag tcactcacaa gaaagtttca atccaggaag
      961 tgcaaaaaga aatcaaaacc agtaatcaaa ccataaaaaa agatattggg gagttgaatg
     1021 aagaaattgc cggtattgca aaaatcatcc aaaaaaacaa acattttgaa cttgaaattt
     1081 atgattttga tttttttgat gagatcatgc aaggaaaatt gaaaacagcc agtgatttca
     1141 attcggcatc caaaagaatc gcttatttac taaataggct gatcgaaaca gaggatttct
     1201 tacgtatgga tgatttgtct gaagaagtcg gtgtcagcag aggaacagtg gtcaaggatc
     1261 taaagacatt aaaagagatg atcaatgatt ttgatgtaaa gattacaggc acaccaaata
     1321 aaggactaag aataatagga gatgagctag aattacgact acttttattt tattttgttt
     1381 ctccctactt ttcagagacg aaaacagaac agtttctaga agaaaaacaa gcaatcaaac
     1441 attttcagaa gaagacgaat gctagtaaac aagaaatcac ccttttaacg aaagttatcg
     1501 gtatttcttt gaatagaatc tctagtaact atctgttgaa gaagcccatc aatcgttaca
     1561 gtgggtgttt tgaatatatc caagaattca atgaactgat caatcagttg gaagaaatct
     1621 atgaactaac tttgagccaa tatgaaaaag attttatcag ttttcctttg aacatcatca
     1681 acacaagtat aacgatgata aggaaagcta actctgattt gcgcttatat ttcgacccca
     1741 tgatgatgcg tattcgacga ttagatctca ttgatctgga tgagtccttc ttatatcaag
     1801 agatgaaaca gcacctctct ttattgataa atcgggtaat ttttcgttat cgattgactg
     1861 atttatttta tggtgagata gaaaaaaagt atcctttttc ttaccaaatc gctagcgaat
     1921 gcatacaagc attagaagct atcttgcaac ataagatctc taaagtagag atcagctacc
     1981 taacgttgta tttggagtta gcatttcgat atcaaaaaga gcatttttcc tctaaaaaag
     2041 tagccattgt tcatacgaca ggaaaaggaa cagcattaat gatccaaaga caaatcaaaa
     2101 ggatattggg gagggatata caaataacac atgtttcaga agaacaatat gaaaaagtag
     2161 atctaaacaa atatttcgct gtttttacaa cgattccgtt aaaaaatgta aatgaagaga
     2221 caccggtgat ccatttgact aatctttttg atgactcttg gttaagggca caatggcaaa
     2281 aagcaaaaaa aaagcgatta gccgggtttc attctactgt ttttttctat caaatatgga
     2341 aagaaaaaaa gacgtatcaa gaatatttaa acaaattagt cgctcctttg atcagaggct
     2401 attatgtgga tgaagaattt gaaaaaagga tcttgaaaag agaatatcag caaagcacat
     2461 ttgttaacaa agaatttgct tttcctcaca caaccaacca gctatctcaa cacattgttt
     2521 tatcagtagg gctaataact gatcaaggtt tagcagaaac aggaggaaat ggagttattt
     2581 ttttatttgc catacctgaa gaaatgacgg ctgaagttga gaatgaatta tttgagctat
     2641 acgatatcct atttgaactc ataaaaatgg gaatagatga gtttattgaa ttagcaccga
     2701 cacaagaatc atttttaaaa ctaatcaaaa gtaaaggaga gagacttcat gaatgatatg
     2761 tatctattca tagttttcgc agcttcagct tatgtgctgc aagcagttat cagttggaag
     2821 caactaaagc atttcaatca aacatatagc gaactgcgaa gaaaaggaaa agtagcgatt
     2881 ggtaaacgac ctgggaaaat ccgttcaggc acgattgtta tgctagctat agacgatcat
     2941 gatcaagttt tagaagtcag aaaaatgcaa ggggttacat ctttggcaaa atttcattca
     3001 ttacctgtct atgcgggtga agatatccat tacatcgatc gttaccatcc ctcagttcga
     3061 gcggaagatc gtttgacaca agcagcttta gaaaatgcca gagaagtata cttacgaata
     3121 gaggcaggaa atgaaaaaag tgaacaacct tcattgcctt ttattgctat gctcaataat
     3181 tttcggatat ggaaaagtac gatcaaagaa aaaaggagtg acaaacgatg gatatcataa
     3241 caaaatttgc tgagggattt atgaggctgt tccaaacagg tgcagaaaca ttcataagct
     3301 ggatgacagg aatcgtacca gttgtcttga tgctgatggt tgcaatgaat acgctgattg
     3361 cttttttagg tgaagaccga gtaacgaaag tagccaaatt atcagcgaaa aatccgctta
     3421 ctcgttacct tgtgttaccg ttcttatctg cttttatgtt gggaaatcct atgtcattta
     3481 caatggctcg tttccttcca gaatactaca aaccaagcta ttatgctgct caagctcaat
     3541 tttgtcatac gagcaatggt gtattccccc atattaatcc tggagaattg tttgtatgga
     3601 tggggattgc tcaagggatc gaaaccttag ggctaaattc gatggagctg gctatccgat
     3661 acttgcttgt gggactgctg atgaacttta ttggtggttg gataactgat tttactactg
     3721 gatttgtatg cagacagcaa ggaatcgttc ttagcaaaaa agtagagctt tcagttgatt
     3781 agagaaagga gagttttaaa tgtatggtga tattaagatc gtaagaggaa acggcgggta
     3841 tggaggtcct ttgattatta ggccgacaaa agataaacac aagttcatct atgtcacagg
     3901 tggaggtgag aggccaatga tcgtagataa aatagaagaa ctgacaggta tggagtctat
     3961 caatgggttc aaaacatcaa tacctgatag tgaaatcgcg cttgcaatcg ttgactgtgg
     4021 agggactctt agatgtggaa tttatcctaa aaaagggata ccaaccatca atatcgtttc
     4081 cacaggaaaa agtggcccat tagcacaata tatcactgaa gatatttatg tttcagcagt
     4141 agggttagaa caaatacagc cactaatgga aactgatgat aacaaagaaa cagtaaatac
     4201 tgcatctcca gataaaaagt acaaatatga tacttctaaa aagataaccg agcaaaaagc
     4261 agagcagcaa aactggatag caaaaattgg aatgggcgcg ggaaaagtag ttgctacatt
     4321 caatcaatca gcaagagaag cagtagatac gatgatccgg acagtgatcc cttttatggc
     4381 tttcgtttcc cttttgattg gtgtgataca aggttctggg atcggtaatg tatttgctaa
     4441 cattatggca cctttagcag gaaatgcagt tggattaaca atcattggct ttatttgttc
     4501 actgccattt ttatctcctt tgcttggtcc aggggcagtt attagccaag tgattggaac
     4561 attgatcggt gtagagattg ggaaaggaaa tattcctccc caattagcat taccggcact
     4621 atttgctatc aatactcaaa atgcatgtga ttttattcca gtcggtctag gtttggaaga
     4681 agcaaaagct gagacagtag aagtaggtgt accatcagtt ctttattcaa gatttcttaa
     4741 cggcatccca cgagttatca ttgcttgggt agcaagtttt ggattatata gctga
//
LOCUS       DASJON010000191         4723 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJON010000191 DASJON010000000
VERSION     DASJON010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.195"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="U3989_002478"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="U3989_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1715896.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="U3989_002479"
     CDS             724..1878
                     /locus_tag="U3989_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1715897.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="U3989_002480"
     CDS             2093..3061
                     /locus_tag="U3989_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1715898.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="U3989_002481"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="U3989_002481"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1715899.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="U3989_002482"
     CDS             4091..4699
                     /locus_tag="U3989_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN1715900.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1457 a          907 c         1109 g         1250 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaa
//
LOCUS       DASJON010000192         4670 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJON010000192 DASJON010000000
VERSION     DASJON010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.196"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..616
                     /gene="rsgA"
                     /locus_tag="U3989_002483"
     CDS             <1..616
                     /gene="rsgA"
                     /locus_tag="U3989_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN1715901.1"
                     /translation="DRFLVTLEYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPV
                     IVPNHTGDTTELIRYFPERLSVFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRH
                     TTRHVELIPIYGGLVADTPGFSSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKE
                     PGCEVKHRVETGGIAQTRYDNYLQFLQEIEKRKPMYAKKEGKRK"
     gene            629..1279
                     /locus_tag="U3989_002484"
     CDS             629..1279
                     /locus_tag="U3989_002484"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN1715902.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            1280..1918
                     /locus_tag="U3989_002485"
     CDS             1280..1918
                     /locus_tag="U3989_002485"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN1715903.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            2073..2927
                     /gene="mreC"
                     /locus_tag="U3989_002486"
     CDS             2073..2927
                     /gene="mreC"
                     /locus_tag="U3989_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN1715904.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            2931..3440
                     /gene="mreD"
                     /locus_tag="U3989_002487"
     CDS             2931..3440
                     /gene="mreD"
                     /locus_tag="U3989_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN1715905.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            3695..>4670
                     /locus_tag="U3989_002488"
     CDS             3695..>4670
                     /locus_tag="U3989_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715906.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         1587 a          862 c          972 g         1249 t
ORIGIN      
        1 tgaccgcttt ctagttacct tggaatacga gtcgatcgaa ccgattattt ttttgacaaa
       61 gaccgacttg tcagacgacc cagaaaaaat cgaagagatc aagcaaacct atactaaaat
      121 aggctatccg gtgattgtac ccaatcatac gggtgatacg acagaactga tccgttactt
      181 cccagaacgg ctatcggtct ttatgggtca gtctggagca ggaaaatcga cgctactgaa
      241 tcaaatatca ccggaactga atttagcaac aggagagatt tctgatgcac ttggaagagg
      301 acggcataca acacgtcatg tggaactgat tcctatctat ggcgggttag tagcagatac
      361 accaggattt agctcgattg actttttgac aattgaagca ggagaactgc cgagacaatt
      421 tcctgaattt gtagaagctt ctgtccattg caaatttaga gagtgcatgc atgctaaaga
      481 acctggctgc gaagtaaaac atagagtaga aacaggtggt atcgcacaga cacgttacga
      541 caattatttg caattcttac aagaaatcga aaaaagaaaa ccaatgtatg caaaaaaaga
      601 aggtaaacga aaataggagg aaacaagaat gaagatagca ccatccattt tgagtgcaga
      661 ttttgccaat ttacaaagag acattgagtt agtggaaaaa ggaggcgcag attacatcca
      721 cgtagatgta atggatggcc gatttgtccc aaatatcaca ttagggccaa atattgttca
      781 ggcaatccgt cctgttacta aattaccgtt agacgtccat ctgatgatcg ttgatccaga
      841 aaactacatt ccagcatttg caaaagctgg agcagatatc attactgtcc acgtcgaagc
      901 aactcctcat attcatcggg cacttcaaat gacaaaaagc ttaggagtta aagctggagt
      961 agttatcaat ccaggaacac cagtctccat gatcaaacat gttttgcctt tggctgatca
     1021 agtactagtc atgactgtca atcctgggtt cggcggacaa agctttattg aagaaacagt
     1081 agaaaaaatt acggaactcg ctgatctacg tagagagaac aattggactt atgcaatcga
     1141 agtagatggc ggaatcgttc cagaaacagc aaaaatctgt aaagatgcag gggcggatgt
     1201 cttcgttgca ggatcatata tctatggagc cgaaaatcca gtcggacaaa tcgaacgatt
     1261 aaaagaggca ttagactaaa tgaaaatttt attagctgct ggaggtaatc cgcaaaactg
     1321 gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg atagaccgtg gtgcgctgta
     1381 cctgcttcaa gcaggctacc cagtagatct ggcagtgggt gattttgatt cattgtcgaa
     1441 cgaagagcag aaggaagtat ttgcaaaagc caaagagtat acccgttccc ctgcagaaaa
     1501 agacgataca gatacacagt taggtatctt aaaaacattt gaacgttacc cgcaagcgga
     1561 agtcactttg attggagcta cgggcggaag attggatcat cttttagcca atctctggtt
     1621 aggtatggaa cctcgcttca aaccctttgc ccatcagctt attatcagag attgtcaaaa
     1681 ttatttgtct tattatttgc ctggagagca tatagtaaga cagataccag atatgaagta
     1741 tttagcttat tgctgcttga ctcccgtctc gaatcttaca ttaaaaggca gcaagtatgt
     1801 attggaaaat aaagaagtag aatatccgac atcttatgca agtaacgaat ttctttcaga
     1861 taccgcagaa ttttcttttt cagatggtat gattgcagtt attcaaagca aagattgatc
     1921 cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg acagaatcta ttatttgcta
     1981 aaaaaaagct taagaggagc aacttacctt ttgtttcacg gcatttagtg ataaaattga
     2041 acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa gttcaatcct aataaaaata
     2101 ttattattac cttggttatt gttatcatcg tggtgacgat actaagtgtg accgctgcgc
     2161 agcgagcgaa tgaaggaaag cccaatttct tccaatccat cgttaatgac agtgttgcaa
     2221 tggttgataa aacaatctct gccccagtac ggtgggtacg aaatggagta gattctgtcc
     2281 ataatctttt ttcaacttat gccgaaaatg agcggctaaa agaaaaaatc gatagctatg
     2341 atgacgtttc acagcaaaac aagaattacg agagagaaat cgaagcgttg aaaaaagaac
     2401 ttgagttgaa tcagacgctg actagttacg aaaaagtaac ggcaaatgtc attactcgtt
     2461 cgcctgacag ttggcaggac atgctgatcg tagacaaggg ttctaaagac ggtatcgaag
     2521 caaatatggc agtcatgtcg caaaaaggat tgattggccg agtgatcgag gtcaatacgg
     2581 cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag ctccaatcat tttccagtac
     2641 gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa aaactatgat gaaaagctcc
     2701 atgccttagt ggtgacccaa ttaactggtg atacggatat caaagaaggg gatgttgtcc
     2761 agacatccgg tcttggaggg aattctccag ctaacttgcc gatcggtacg gttattaaaa
     2821 cgaaaccaga tagttatggg ctggatcggg aagtttatgt gaaaccttat gcagaaatgt
     2881 atgacgtgtc agttgtgacg attgtccaac ggctagcgga ggattagtcc atgttgaaga
     2941 aagaaacaat gaagtactat ctgccaatcg ttttgttctt tttgatgttg atagatggtc
     3001 atttaacaag aatgctaggg gagtggtcga aaggcaccta tatgtcaaat gcccactttc
     3061 tgatattggc attattatgt tgcagtatgg cgtttgaaaa acgttattta ctgattacca
     3121 cgattgttct cggggctatc tatgacgctt actatattgg cgttatcggt atctatgcag
     3181 tagctctccc tttaattgta tggttgatgt atgtaatgaa agacgttatc catgtcaaca
     3241 tctttactga atttttcagt atgatcatct ttgtcacggg ttatgaattg tttacgatgg
     3301 tggtccagtt gatttttaaa ttagcagtag taaataacac gtattttatt acaaggtttt
     3361 taggacctac actgctgttg aacatgatta tatttgtatt attcattttt ccctttaaga
     3421 aattattcag tgatgaataa tttctttttt tatttttagg tcgaaagcga aaaagaatcg
     3481 ttgttatctt aagaaatgct agacttaacg cggctttttc atgatcattt tctgaaacga
     3541 acagttttgt catttttaaa agttgttttg aaacattttt gtaacataac tattatatcg
     3601 ttgacacact gctatggtac aatttttata tcgttaagaa agacttaaat atatttagat
     3661 atttattaaa ttaagccagt cggaggaatg aagagtgaaa aagagtttaa tatcagcagt
     3721 aatggtatgt tcaatgacct tgactgccgt agcatcaccg atcgctgccg cggcggacga
     3781 ttttgattct cagatacaac agcaagatca aaagattgca gacttgaaaa atcaacaagc
     3841 agatgcacaa tcacaaatcg atgccttaga aagccaagtt tctgaaatca acacacaagc
     3901 acaagaccta ttagcaaaac aagatacatt gcgtcaagaa tctgcacaat tagtaaaaga
     3961 cattgcagat ttacaagaac gtattgaaaa acgtgaagat acaatccaaa aacaagcacg
     4021 cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat gctgtattga atgctgattc
     4081 attagcagat gctattggac gcgttcaagc aatgacaacg atggtaaaag caaacaacga
     4141 cttgatggaa caacaaaaac aagataaaaa agctgtagaa gacaaaaaag ctgaaaacga
     4201 tgctaaacta aaagaattag ctgaaaacca agctgctttg gaatctcaaa aaggcgatct
     4261 tttatctaaa caagctgatt tgaacgtact gaaaacttca ttagctgctg aacaagcaac
     4321 tgctgaagac aagaaagctg acttgaaccg tcaaaaagca gaagctgaag cagagcaagc
     4381 tcgtattcgt gaacaacaac gtctagcaga acaagctcgt caacaagctg cacaagaaaa
     4441 agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca caagctactc aagcttcatc
     4501 tactgcacaa agctcagcaa cagaagaatc atcagctaca caatcttcaa tgactgaaga
     4561 atcatcatca gctacacaat cttcagcaac agaagaatca acaacacctg aatcttcaac
     4621 agaagaatca acagcgcctg aatcttcagc aacagaagaa tcaacaacag
//
LOCUS       DASJON010000193         4651 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJON010000193 DASJON010000000
VERSION     DASJON010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.197"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..786
                     /locus_tag="U3989_002489"
     CDS             <1..786
                     /locus_tag="U3989_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1715907.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(857..1756)
                     /locus_tag="U3989_002490"
     CDS             complement(857..1756)
                     /locus_tag="U3989_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1715908.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            1925..4096
                     /locus_tag="U3989_002491"
     CDS             1925..4096
                     /locus_tag="U3989_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1715909.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            4164..4505
                     /locus_tag="U3989_002492"
     CDS             4164..4505
                     /locus_tag="U3989_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1715910.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT         1524 a          861 c         1026 g         1240 t
ORIGIN      
        1 aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt tgaatttttt
       61 gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg tatcgacaaa
      121 acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc acaattcgat
      181 tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac ggatgtcaat
      241 gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag ttgtatttac
      301 cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg
      361 atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga ccattatgaa
      421 ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc gtctattgag
      481 gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg ggctattcag
      541 gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc tgaaaagatt
      601 attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg tcagcaattt
      661 gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg
      721 ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca cgaacatcaa
      781 aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc tgataagact
      841 ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact attttttctt
      901 gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt tcagctgttc
      961 tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa tgaactctga
     1021 tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca gaaagtactt
     1081 tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg accctttttc
     1141 tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg gcaatttctc
     1201 cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt cttccaagta
     1261 tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct ttgtccaagc
     1321 ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa tgattgagta
     1381 aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc ttatccacta
     1441 taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct aattgaactt
     1501 cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg ccctttactt
     1561 catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata cgcaaaaagt
     1621 gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag
     1681 gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca gtgcaatcgc
     1741 agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt tctgttcatt
     1801 cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac ttaagcagga
     1861 agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac aggagaagaa
     1921 gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca gtttttggcg
     1981 ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct ggaaaaaata
     2041 tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga caagtatcta
     2101 cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat taagccaatc
     2161 gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac
     2221 atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct cgacagaaga
     2281 taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg cagcagtccg
     2341 aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc agcagcttgc
     2401 caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg aattattttt
     2461 ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt atcttaacaa
     2521 tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt attttggagt
     2581 agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc
     2641 tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa acacagtact
     2701 aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag aaagccagac
     2761 agatatcagc gcgtatctta atgacacata cgataattca gattctggtt atcgttatcc
     2821 ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg atgaagaaga
     2881 tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc aaaagcaatt
     2941 agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg gtgccatggt
     3001 ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac tagtaggacg
     3061 aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc gttcaccagg
     3121 atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat acaagccttc
     3181 cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg acgggacata
     3241 ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg
     3301 gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat tcggcctgcc
     3361 tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa aaggagtatc
     3421 tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga tgtatacgcc
     3481 gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata acacaaacaa
     3541 aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca tgcttcttgg
     3601 tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga tagcagggaa
     3661 aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat ggatgatcgg
     3721 ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca gcaagctgca
     3781 ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg
     3841 catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg ccacaggagg
     3901 acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag agtctggaaa
     3961 ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga aaaacttcgg
     4021 cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc tctggcggaa
     4081 atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata gagagtaact
     4141 actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat caaatcgaac
     4201 gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag aaagtacaag
     4261 caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc gaattacaag
     4321 aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca caagaaatgg
     4381 ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag caaggcttca
     4441 gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac ctttacagcg
     4501 aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc ttgagcgatg
     4561 acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc aagaggtcgt
     4621 gagcctaccg tttaactctg agttacaagg a
//
LOCUS       DASJON010000194         4587 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.198, whole genome shotgun sequence.
ACCESSION   DASJON010000194 DASJON010000000
VERSION     DASJON010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.198"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(370..1089)
                     /locus_tag="U3989_002493"
     CDS             complement(370..1089)
                     /locus_tag="U3989_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN1715911.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            1426..1758
                     /locus_tag="U3989_002494"
     CDS             1426..1758
                     /locus_tag="U3989_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715912.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1745..3748
                     /locus_tag="U3989_002495"
     CDS             1745..3748
                     /locus_tag="U3989_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN1715913.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3767..4237
                     /locus_tag="U3989_002496"
     CDS             3767..4237
                     /locus_tag="U3989_002496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN1715914.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            4258..>4587
                     /locus_tag="U3989_002497"
     CDS             4258..>4587
                     /locus_tag="U3989_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN1715915.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNAL"
BASE COUNT         1524 a          797 c         1006 g         1260 t
ORIGIN      
        1 ccagctcaaa aggctgttgg actatcgaaa ggacgattga gaagaattcc agtgaacatt
       61 gttccataga ctggagactg agacaaaagt cgtgtcgcag tctctttttc cggataaacg
      121 gtgttcttct gccaactcct cggtcacaag tcacactatt caacaaacat gaaaagctgt
      181 tttttgaaat tgctccttgt gactcagagt tgaacggcag gctcacgacc tcttggtata
      241 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag
      301 ccattttcac aaaatttttc ttaatactcg aaactaaaca gctttttcac aaccccctca
      361 gaaagtctat taaaattcat tcacaaagaa atcgaataag cgctgttcag atggatgtac
      421 acggtgggta agttccggat gccattgaac accgaggatt ttatgcatat catctttgct
      481 ttgaaccgct tcaacgagtc catcgttaga aaaagcgatg gcttttaata aaggtgaaag
      541 atctttgact gcctgatgat ggtaagaatt cacaaaatat ggatcgggga gaaggttgct
      601 taacaaactg tctgaaacaa tcttgacttc gtgagtcgta aattgcgggg cagtgggctg
      661 ttgttcatgt tttactgacc actttggata taatgaaaga tcttgataaa gtgtaccttc
      721 caaaacgaca ttcaatagtt ggagtccccg gcagactgca aaaattggtt ttctttgttt
      781 caatgcttcc aagatcagtg cctgttcaaa ttggtctcgt tgaatatttg tttccgttag
      841 ctttggatga ggatcttcca tgtaaagttg tggactgaca tcttgaccac ctgcaagaat
      901 aagtttgtcg atttgtccaa tcagttgagg agctgaatct ggagacatga ctggcagaat
      961 aagaggcaat ccttgagcat ctaaaactgc atccacgaat ccttgcggtg tatagctcac
     1021 ttgatttcct tggaaagtat ctgttgctcg aatcagttga tttccggcaa tgccgatgat
     1081 tggtttcatt tgtattcccc ctaaagtaga cttattgtaa caaggattta aaaaaaatta
     1141 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
     1201 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
     1261 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
     1321 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
     1381 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
     1441 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
     1501 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
     1561 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
     1621 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
     1681 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
     1741 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
     1801 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
     1861 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
     1921 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
     1981 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
     2041 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
     2101 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
     2161 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
     2221 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
     2281 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
     2341 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
     2401 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
     2461 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
     2521 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
     2581 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
     2641 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
     2701 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
     2761 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
     2821 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
     2881 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
     2941 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
     3001 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
     3061 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
     3121 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
     3181 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
     3241 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
     3301 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
     3361 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
     3421 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
     3481 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
     3541 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
     3601 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
     3661 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
     3721 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
     3781 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
     3841 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
     3901 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca
     3961 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
     4021 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
     4081 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
     4141 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
     4201 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
     4261 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
     4321 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
     4381 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
     4441 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
     4501 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
     4561 caaatccagt ttatcaaaaa tgcattg
//
LOCUS       DASJON010000195         4581 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJON010000195 DASJON010000000
VERSION     DASJON010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.199"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(299..1756)
                     /gene="ascB"
                     /locus_tag="U3989_002498"
     CDS             complement(299..1756)
                     /gene="ascB"
                     /locus_tag="U3989_002498"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1715916.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1753..3648)
                     /locus_tag="U3989_002499"
     CDS             complement(1753..3648)
                     /locus_tag="U3989_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1715917.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3706..>4581)
                     /locus_tag="U3989_002500"
     CDS             complement(3706..>4581)
                     /locus_tag="U3989_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1715918.1"
                     /translation="AFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTRMDAGHNTMNH
                     FVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFRKQGEILIVSN
                     QPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTTEQELIFEWDD
                     LIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKIHKKIDSIQQL
                     ISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKRVFFVSFDYKR
                     NDILVFFQAVSDLLG"
BASE COUNT         1386 a          958 c          755 g         1482 t
ORIGIN      
        1 atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa acaaaaatag
       61 ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg tacaccgttt
      121 attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca
      181 gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg agaatactgt
      241 ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt tccttttatt
      301 ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat tttttcttta
      361 accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat ccataacgtt
      421 ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac gtataaccaa
      481 ataaatctac tccatctttg atagcttcct tcatctgctc aatatgctgc ttcaaatagt
      541 caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata gctccgagtc
      601 cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc aaagcaattc
      661 tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac ggatttttaa
      721 ttcctccagt tattaagttt ccttggaccc cttcccaatt ttctggatga gcactgactg
      781 cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct aagatttctt
      841 catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt tgcgtatacg
      901 ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt tgctgctcaa
      961 ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga tagctgatca
     1021 tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct aaagcacttg
     1081 ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga tggttttctt
     1141 caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca ttgaaagtca
     1201 tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa
     1261 actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat aacggcattt
     1321 cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg tcaaacactt
     1381 gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga ggaaaaattc
     1441 ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat aaacggatgt
     1501 cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa tacttgctgt
     1561 tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga tctgtccatt
     1621 tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat cctttttcat
     1681 ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat aaaaatcctt
     1741 ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt gattattttt
     1801 cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa ccattgtggt
     1861 cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata aatcgccttt
     1921 acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta aattcactgt
     1981 atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc caattgcatg
     2041 tttcgttgga aaaactgttg aaacttcccc gtttacagaa ctgtagattt ttccgtccgt
     2101 tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat cgtttacttc
     2161 agataaatca attatttcac ctgctgcagg agtacttaac actatatcct ttgaggattc
     2221 ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac tgatcatcgc
     2281 taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg ggaaagacaa
     2341 aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa
     2401 tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag tcacaccata
     2461 aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag ataaactttt
     2521 tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt gcgcagctac
     2581 ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat tgttggtcat
     2641 tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa atccgccgat
     2701 aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag ctgcaatcag
     2761 ctgacttgca taatctgcaa ttggacctac aactaataaa ataattggga ccatcacgat
     2821 gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt tgcgaatctt
     2881 aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg tacttgtata
     2941 ccttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac tatatgcact
     3001 gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac gatctgtatt
     3061 gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa atgcatcagc
     3121 tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca aactcgtaca
     3181 taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag tcggggcaaa
     3241 aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag ttcctctctt
     3301 tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat acacttcatt
     3361 tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag tgatcacacc
     3421 ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg taaagcgtaa
     3481 ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa atcggacaat
     3541 atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta atgccgtagc
     3601 aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc aattagtacc
     3661 ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc
     3721 actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg agacaaaaaa
     3781 aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat actctgtgat
     3841 ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat ttaacggaag
     3901 cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct ttttatggat
     3961 ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct ttttttcttc
     4021 aactatggtc agcttttcac gcaaaatctg ataaatctcc caagattcaa actcatctag
     4081 aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct cagtagtcaa
     4141 aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca ctggttctat
     4201 ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta gtgtattcaa
     4261 aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa aaggattcaa
     4321 cgcttcggca agatacaaaa caaaataagc gctttcagct aaattgggac gtaattgaaa
     4381 atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg agatgatctt
     4441 ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca tttgtttgat
     4501 atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat ctaaaaaacg
     4561 aataatcgtt tgctcaaatg c
//
LOCUS       DASJON010000196         4556 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJON010000196 DASJON010000000
VERSION     DASJON010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.200"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            372..602
                     /locus_tag="U3989_002501"
     CDS             372..602
                     /locus_tag="U3989_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN1715919.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            699..2000
                     /gene="murA"
                     /locus_tag="U3989_002502"
     CDS             699..2000
                     /gene="murA"
                     /locus_tag="U3989_002502"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1715920.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            2013..2189
                     /locus_tag="U3989_002503"
     CDS             2013..2189
                     /locus_tag="U3989_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1715921.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2324..2620
                     /gene="yidD"
                     /locus_tag="U3989_002504"
     CDS             2324..2620
                     /gene="yidD"
                     /locus_tag="U3989_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN1715922.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2684..3067
                     /locus_tag="U3989_002505"
     CDS             2684..3067
                     /locus_tag="U3989_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715923.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            3236..4120
                     /locus_tag="U3989_002506"
     CDS             3236..4120
                     /locus_tag="U3989_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1715924.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4234..4470
                     /locus_tag="U3989_002507"
     CDS             4234..4470
                     /locus_tag="U3989_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN1715925.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1540 a          697 c          993 g         1326 t
ORIGIN      
        1 gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt ctggaacacc
       61 gtatatcaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac aaatggaaaa
      121 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga ttgaacaccg
      181 tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt ttgcatattt
      241 ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta tacttgtttt
      301 tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca atcgttagaa
      361 aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg agtcatttgg
      421 cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact tttttcagac
      481 caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt ttagggtaca
      541 ctgcaagtaa ttttttctta gaagttatcg ctttatgccg aaatcttttc gttagcctgt
      601 aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa agattttgag
      661 taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc atcgtaagag
      721 gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct gttttgccta
      781 ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac gttccgattc
      841 tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat gtagattttg
      901 atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc gaagcgcctt
      961 atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta cttgccagaa
     1021 atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga ccaatcgatt
     1081 tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac ggctatatcg
     1141 aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct agtgtgggtg
     1201 ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc attgaaaacg
     1261 tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg ggcgctcaag
     1321 tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta catgcagtca
     1381 accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct gctgcaatga
     1441 cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taactgccca ttgatttcta
     1501 aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt gtcatcggac
     1561 caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc cctacagata
     1621 tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg gtgactgaaa
     1681 cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac gctcatgtca
     1741 aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt gcagaagtat
     1801 atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa gcaaatggaa
     1861 tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc catatcaaac
     1921 tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct gctgaaaaaa
     1981 cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat atcttattaa
     2041 ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg gccggaacga
     2101 tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag ccaagtttgt
     2161 ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt ctagtcgata
     2221 tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt tgacgttagt
     2281 cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga agatattgat
     2341 ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta gctgccgtta
     2401 ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg cgctaaaagg
     2461 gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag gcggtatcga
     2521 ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag aaccagatta
     2581 taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag gaaatttatt
     2641 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2701 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2761 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2821 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2881 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2941 gctagcttca aaccaaactc ggatcattat acgtactggc actactagta gcgctgcgtt
     3001 acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3061 tcattaatct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3121 aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa aatgctttca
     3181 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3241 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3301 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aaacgcaatt
     3361 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3421 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3481 tcatggattt aatattttcc gagatttgga aactctgttt caactcccag taacgattga
     3541 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gtgattatca
     3601 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3661 aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct tagatggcga
     3721 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3781 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3841 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3901 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3961 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     4021 tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc ttgtaggtgc
     4081 agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga gaaggtgcag
     4141 cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4201 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4261 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4321 ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa agaatatgaa
     4381 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4441 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4501 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa accttt
//
LOCUS       DASJON010000197         4538 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJON010000197 DASJON010000000
VERSION     DASJON010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.201"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1014..1304)
                     /locus_tag="U3989_002508"
     CDS             complement(1014..1304)
                     /locus_tag="U3989_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715926.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="U3989_002509"
     CDS             1706..1942
                     /locus_tag="U3989_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715927.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="U3989_002510"
     CDS             1949..3901
                     /locus_tag="U3989_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1715928.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="U3989_002511"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="U3989_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1715929.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1554 a          797 c          750 g         1437 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcat
//
LOCUS       DASJON010000198         4507 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJON010000198 DASJON010000000
VERSION     DASJON010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.202"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(3..86)
                     /locus_tag="U3989_002512"
     tRNA            complement(3..86)
                     /locus_tag="U3989_002512"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Leu,seq:cag)
     gene            614..1114
                     /locus_tag="U3989_002513"
     CDS             614..1114
                     /locus_tag="U3989_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1715930.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1304..1648)
                     /locus_tag="U3989_002514"
     CDS             complement(1304..1648)
                     /locus_tag="U3989_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN1715931.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1802..2722)
                     /locus_tag="U3989_002515"
     CDS             complement(1802..2722)
                     /locus_tag="U3989_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1715932.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2909..3400
                     /locus_tag="U3989_002516"
     CDS             2909..3400
                     /locus_tag="U3989_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN1715933.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3424..>4507)
                     /locus_tag="U3989_002517"
     CDS             complement(3424..>4507)
                     /locus_tag="U3989_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN1715934.1"
                     /translation="AKEDHWDYHHQKAPAESLVKAGYQLSEKIKEWLAEFPEHPSQEQ
                     LLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDLIVKEFCIDPSLFLEQSLDKGKSSIL
                     FSASFSPLSYYQETLGGQKSLAYKLPSPFPEGNQQVFIANYLETTYRKREHSLAKLVE
                     TIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQYPDSNVLIQETDMNEEERERFLTKF
                     QTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSMIVGVGLPQMNHEQELIKSYYDEKEH
                     LGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVVLADQRFSSFSYRRLFPQHWQTAREV
                     RSVGELEEGIQRFWLSKEAVNQKNEE"
BASE COUNT         1427 a          877 c          760 g         1443 t
ORIGIN      
        1 tatgcggcta agaagactcg aacttccacg aggtcaccct cactagcgcc tgaagctagc
       61 gcgtctgcca attccgccat agccgcatca catcttttag tttaggatta catgtgtttt
      121 ttgtcaagaa catatttttg ttctttttca tttctgtacg ccctccctta tgccatctac
      181 acaaataatt catccgagca actccatcat ctattgaggg ttggtgaaaa aatctgtttt
      241 atccatatga tcgatttcaa tgttaaacga acgactctgg gattagtaac tattttattt
      301 ttttcttcat ttcttttttc ctctttttct cgattcagct atcttctttc atttggatat
      361 tgattatttc ttctgttgat tactatcctt ctggaattct agacgctgaa gtaattcacg
      421 ttttctttct tctagagatc ataagtaacg aatgaacgaa cttcaaataa tttttttatt
      481 gtaattcata tttgtattgt tcgatgattc ttaatacgat atgacacaat aaataaaaat
      541 aggcatggct atttacaaca atagtgctat agtagaaaac aaatgaataa ggttacttaa
      601 agaggtgaat acgatgaaac tgatcaaact atgggaagca gatttaaaga aagcttatca
      661 gctacaaact agctttggac caaatgagag tggatttatc aacaatgctt atggctatac
      721 atttgctgaa tttttagatt atgtaaaaaa atgcgaaaaa acgtctttag gaatcgatct
      781 tcctgagagt tacgttcccg caaccgtctt tattttagaa agtgatgaac atgagtatgt
      841 aggaattttt aatttgcgcc ataaactgaa cgaaacatta agaaacggcg ctggccatat
      901 tggttacggc atctccccta attatcgtaa aaaaggatat gccacacaag gattagcatt
      961 agtactacaa gaggcaaaaa aaatcggcat tgatgaggca cttttgtctg taaataaaaa
     1021 aaatcttggc agtcttaaag cacaacaaaa aaatggggct gcgatttatc gtgaagatga
     1081 gaaagaatac tatacgaaaa tcaaactgat gtaaaaaaga aggattccaa aaggaaaggg
     1141 atttgaatgt cctttatcaa tagaacacat ttatttaatt aacttataat atagcagaca
     1201 aaattttata aacaaagaaa ccttttatct cttttcttct agaaaggata aaaggctttt
     1261 ttgtttttcc tatttgcttc tcatagctag tcagccaaga tttctatcgt atccccaacg
     1321 caatccgtgc ataacggctc atcttatctg tcgtccatgc tggataccag actagtttga
     1381 cttctgcttt cgttacttct ggcacttctt ttaatgcgtc atggatagat tctgttaaaa
     1441 tatctgctaa tggacagccc atagttgtta gagtcatctt aatgactgtt tctccatttt
     1501 caggattgaa ttctatatca taaatcaagc ctaaattgac gatgtcgatt cctaattcag
     1561 gatcaatcac catttccaag gcagcaagaa tacgttcttt gattagttct atttcttgtc
     1621 cagaccgttc ttggtttgct tcgctcatcg tacgacactc cttaacacga tttttattta
     1681 tcatactctt tttttagctg cttgtaaatg agaatttaga agtaagattc tcgtttaaat
     1741 ttataaaaat gttcttatta gatgtaatag attaaattta ttctaaaata aacctgattc
     1801 ctcatttttt ttgtgaacga ttttttattt ctggcaatac gccagttgtt cgatacattt
     1861 tttgttcgtt ttcatttgca aaccggccaa atactgaatg agttggtaaa atattgataa
     1921 atgcatcgtc gtcgatttca tgaacgatct gatcaagatc atacatttcg tagcgggtga
     1981 tgaccatcat gatcatgttc ccttgttttt ttgaatagcc accgacagac ggcagtaatg
     2041 tcattccacg taccatccgt tttgctagtt cttctgttac ctcttctggc ttgacggtaa
     2101 cgatcattgc tgttactttt tgatgactcg tgtggatggc atcaattact tgagtcattg
     2161 cataaatgga aataatcgta tacaaggcac tttcccagtt aaagaagaag cctgcgatga
     2221 ctacaatgat accatttaga atcagcatgt agtttccgac tgtttttcca gttgtttgtg
     2281 aaagtacaag agagatgata tccatcccac cagttgtaaa gcccattttt aaagataatc
     2341 ctacgccaag acctaataaa acaccgccta ctagggcatt catcaaaata ttatcagtga
     2401 tctgtccgac tggaagcagc atcgtcatca ccgatacaca tacaacattc acaaaactaa
     2461 ggatcgttgc tcgttttcct actttgagaa accctaatac aaacacaggg atattcaaaa
     2521 gaaaaataaa tgttcctgtg tctatcttga tatgaaacca ttcatataaa agcgaggaaa
     2581 tgatttgagc gatcccattc atccctgcag aaaagacatt ggctggaatc aaaaaataat
     2641 ttaaagcgac cgcagcggtc aatccggtta tcaaaataac cactattttc tttaatcttt
     2701 catttttatg atacgcagtc ataattttac tcacaagcac ctctccccta ctttggtatt
     2761 ccaaatgtaa gaataacagt ttatctatta aaataaaata cttcgaactc aaatgagaag
     2821 aattttagca aacaggtaag aatctgccct aatcctatga taagatagat gtatggtaaa
     2881 tatttatatg tatatcagga gggttcctat ggcaaaagat gctagcttcg acattgtatc
     2941 agaagtcaat acagaagaag taaaaaatgc gattcagcaa acaacaaaag aattgtccaa
     3001 tcgctttgac ttcaaggatt ctacagtaga aatcaaatta gagaacagca atctagttgt
     3061 attgagcgat gatgatttca aaatcgaaca gatcaaagat gtattatttg gtaagctgat
     3121 caaaagaaat gtgccaatca aaaatatcca tttttccgaa tcagaacacg ctctaggtgg
     3181 caaagctcga caaactgccg atttggtcaa cggaatcgat cgtgaaaacg cgaagaaaat
     3241 cactacagct atcaaaaatg aaaaactgaa agtaaaagca caaatccaag aagatcagat
     3301 tagagtgaca ggtaagaacc gtgatgatct acaagctgtg attgctttat tacgtaaact
     3361 tgacttacca atcgagttgc aattcactaa ctatcgttaa tcaaaaaacg aggctgacgt
     3421 ctgtcattcc tcgttttttt gatttactgc ttcttttgat aaccaaaaac gctgtattcc
     3481 ttcttccaat tccccaacac ttcgtacttc tcttgctgtt tgccaatgtt gtgggaataa
     3541 acgtcgataa gaaaaactag aaaatcgttg atctgccaat acaacgattc cttgatccgc
     3601 catgccacga atcactcgac caccagcttg caagactttg ttcattccag gaagctgata
     3661 agcgtaagca aatccaaggt gttccttctc atcataatat gatttgatca gttcctgctc
     3721 atgattcatc tgcggcaaac ctacacctac gatcatactg ccgatcaatc ggtcccctct
     3781 caaatcgatt ccttctgaaa aaatcccgcc aagtacgcaa aatccaatca atgtttcagc
     3841 gggatttgtt tgaaattttg ttaaaaatcg ttcgcgttct tcctcattca tgtccgtttc
     3901 ttggattagc acattgctgt cgggatattg ctgtctgaat gcttccaccg caagatctaa
     3961 atattgataa gaaggaaaga aaacgaaata attcccgacc tttccatcag caaaagcatg
     4021 aatcgtctct actaattttg ccagactgtg ttcccttttt cgataagtcg tctctaggta
     4081 atttgcgatg aaaacttgct ggtttccttc tgggaaagga ctcggcagtt tgtaagccag
     4141 acttttttgt ccccccagtg tttcctgata ataagagagt ggtgaaaaac tagcagaaaa
     4201 aagaatactg ctttttcctt tatccaacga ctgctcaaga aacaagcttg gatcaataca
     4261 aaactctttt actatcagat cgcgatacgt tttttcgact gttgtttcgt agtgatcatc
     4321 ataaaattca ctgaccttca aaaaatgaag caaatcaaaa taataaggaa gcaattgttc
     4381 ttgtgatggg tgctcaggaa attctgccag ccattcttta attttttcgc ttaattgata
     4441 acctgccttt actaaagatt cagccggtgc tttttgatga tgataatccc aatgatcttc
     4501 ttttgct
//
LOCUS       DASJON010000199         4478 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJON010000199 DASJON010000000
VERSION     DASJON010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.203"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..461
                     /gene="gatC"
                     /locus_tag="U3989_002518"
     CDS             156..461
                     /gene="gatC"
                     /locus_tag="U3989_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN1715935.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            461..1933
                     /gene="gatA"
                     /locus_tag="U3989_002519"
     CDS             461..1933
                     /gene="gatA"
                     /locus_tag="U3989_002519"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN1715936.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            1930..3360
                     /gene="gatB"
                     /locus_tag="U3989_002520"
     CDS             1930..3360
                     /gene="gatB"
                     /locus_tag="U3989_002520"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN1715937.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            3370..4425
                     /locus_tag="U3989_002521"
     CDS             3370..4425
                     /locus_tag="U3989_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1715938.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
BASE COUNT         1506 a          819 c         1007 g         1146 t
ORIGIN      
        1 aagatcaaat ggcagatgca ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt
       61 ttttcagaca aatttcaata aacaatttca gacacggcac tcttgtgtta aaatagagag
      121 caaagttttt aactcgttta gaaagaaggg gtagtatggc gattagtgaa gaacaagtga
      181 aacacgttgc caaattagca aaacttgcgt tcgcagatga agaacttgca agttttaccg
      241 accaattagg taaaatcatc gatatggttg aaatgctgga agaagtagat acggaaggtg
      301 ttccatttac gtcgaatgtg gttgaaacag taaacgtgat gcgacaagat cgggcgcaag
      361 ctggatggaa ccgcgatgaa ttattgaaaa atgtgccgga aacagaagac ggctttatca
      421 aagtacccgc aatcatcgat aatggggagg ctggcgcata atgacagaat tacacaatcg
      481 ttctctagaa gaactgcatc atatgcttgt atcgaaagaa atcactgtac aagatttgac
      541 aaaaacaaca tttgaacgaa tcaaagaaac agagcctgag gtcgaggcat tcattacatt
      601 gaacgaagaa aaagcattgg aacaggcaaa agctttagat gaaaaaggga ttgatgaaag
      661 caacgtcctt gctggtattc caattggtat caaggataat atcgtaacaa aagatatctt
      721 gacaaccgct gcatctaaga tcctttacaa ttttgaacca atttatgatg caacggtcat
      781 ggataaaatc tatcaagcag atatgatccc tgttggtaag ctaaacatgg atgaatttgc
      841 tatgggtgga agtacagaaa cttcttattt caagaaaaca aaaaatgctt gggacccaac
      901 aaaagtacct ggtggctctt ctggtggttc tgcagcagca gttgcagctg gacaaattcc
      961 agtttcttta ggtagtgata ctgggggaag tatccgccaa ccagcagctt ttaacggcat
     1021 cgtagggatg aaacctactt atggccgtgt ctcacgtttt ggtttgattg cctttgcttc
     1081 atctcttgac caaatcggac caatgacccg tacggtgaaa gataatgcat tagcattgac
     1141 agctatcagc ggttatgacg aaaaagacgg tacttcttca ggcgtttcag ttcctgattt
     1201 tgcagaggga ctgacaggcg acatcaaagg aatgaagatt gctttaccta aagaatactt
     1261 aggcgaaggt gtggcccctg gcgtaaaaga agctgtattg aaagcagcag aaacattccg
     1321 ttcattaggt gctgttgtgg aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt
     1381 ttattatatc attgcctcat cagaagcaag ttccaatttg caacgattcg atggtatccg
     1441 ctacggttat cgctctgaaa atgtgaaatc attagatgat gtatatgtca actcacgttc
     1501 agaaggattc ggtgaagaag taaaacgccg tatcatgtta ggtacattct ccttaagcgc
     1561 gggttattat gacgcttact tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga
     1621 ttttgaaaaa gtctttgcag attatgactt gatcatcggg ccatcttcac caacagttgc
     1681 attcggaatc ggtgagaaca ttaacgatcc aatcacaatg tacatgagtg acatcttaac
     1741 gatccctgtc aacttggctg gacttccagg tatgtctgta ccggcaggtt tatcagaagg
     1801 attaccagtt ggattgcaga tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc
     1861 ggcatatgcg tttgaacaag caacagaatt ccataaacaa caaccagcta ttttaggagg
     1921 gaaagacgaa tgaattttga aacagtcatt ggactagaag tccacgtcga gttaaaaacc
     1981 aactcaaaaa ttttctctcc agcgccagct cattttggtg cagaaccaaa cagcaataca
     2041 aacgtgatcg actggggtta tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa
     2101 ttcgggatga aagcagcttt ggcattaaac tgtgaaatct ctaaagatac tcattttgac
     2161 cggaaaaatt acttttaccc agataatcca aaagcatatc aaatttccca atttgatcaa
     2221 ccaatcggtc atgacggatg gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg
     2281 atcgaacgtg tccatttaga agaagatgct ggaaaaaaca tgcatggtat tggcggttat
     2341 tcttacgttg atttgaaccg acaaggcacg cctttgatcg aaatcgtttc tgaagctgat
     2401 atgcgttcac cagaagaagc atacgcttat ctagaagcat tgcgatcaat tatccaattt
     2461 acagaagtca gcgatgtgaa aatggaagaa ggctctatgc gctgtgatgc caatatttct
     2521 cttcgccctt atggacaaga agagttcggt acgaaagcag aattgaaaaa cttgaactct
     2581 ttatctttcg tgaaaaaagg tttagctttt gaagaaaaac gtcaagcaaa agttcttctt
     2641 tcaggaggag aaatccaaca agaaacacgc cgttttgacg aaacaacgaa taaaacagtt
     2701 ttgatgcgtg tcaaagaagg gtctagtgat tatcgttact tcccagagcc tgatatcccg
     2761 aaattcgtga tcgatgacga atggattcaa aaagtaaaag ctagtttgcc tgagatgcca
     2821 gcttctcgtc gcgaacgcta tatccgtgag tttggacttc cagagtatga cgcaatggta
     2881 ttgacactta caaaagaaat gtctgatttt tttgaagctg ttttggaaaa tggtgcagat
     2941 gccaagcaag catctaactg gttgatgggt gaagtttctg cttacttgaa cagtgagaaa
     3001 ctggaattag ctgagacaaa attgacacct gagaacttag caggaatgat tcgtttgatc
     3061 gccgatggaa cgatcagctc aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat
     3121 ggcggagaag cccaagaagt agttgaaaaa aatggttggg tgcaattatc tgatccgaac
     3181 aaactactgc caatcattaa tgaaattttg gataacaacc aacaatctgt cgatgatttc
     3241 aaaaatggaa aagatcgtgc ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa
     3301 ggacaagcaa atcctggtgt cgtgaataag ctacttaaag atgaattagc gaaacgatag
     3361 aggctgaaaa tgaaaaaagc acgcgtaatt tataacccca cctcgggaaa agaattattg
     3421 aaaaaaaatt tggcggatat cctacaggca ttagaagaag caggatttga agccagcgcc
     3481 tatgcaacta caccagaacc agattcagcc aagaatgaag catgtagagt cgcagaacta
     3541 ggttttgatc tagtagtggc agctggtggc gatggcacaa tcaacgaagt ggttaatgga
     3601 atcgctccgc ttgaacaccg tcctaaaatg gccatcatcc cagctggtac aacgaacgac
     3661 tatgctcgag cattaaaaat tcctagagat aatattaaag cagcagcaga agtgataaaa
     3721 aaacaccaaa ccgtaaaaat ggacatcggc ctttctgata acagttactt catcaatatc
     3781 gctgcaggtg gctatttgac agaattgact tatgaagttc catcagaact aaagagtatt
     3841 tttggttatc ttgcttatct tgcgaaagga gcagagatgc tccctcgagt aaagccaatc
     3901 aaaatgcgct tgaagtatga tgaaggggaa tatatcggaa atgcttcgat gtttttccta
     3961 gggctgacga actctgttgg aggattcgaa cagatcgcgc cagatgccaa attggatgat
     4021 gggaagtttt ctttaatcat tgtgaagaca gcaaatatct ttgagatctt gcatattgca
     4081 gcactcatgc tgaatggcgg tcgacatgtc gatgatccaa gaataatcta taccaaaaca
     4141 agcagtttgt tcgttgaaac agaagatgat atcaatcgac cagtaatgat caacttagat
     4201 ggtgaatacg gcggggaagc accgatgcat ttccaaaatc ttcatcaaca cattgagttc
     4261 tttgccaata ccgatcagat tcctgatgaa gcaatcacag gtacagatga agaagaatta
     4321 gaggttgtca gcaaagaatt cgtaaaagaa gtcgaacgtt taacggatga agatatcgac
     4381 ggtgacggaa aaatagcaga cgaaacacct aatcaaaaga attaatcaaa agccggtgct
     4441 gaacgtagta aggtcagtgc cggttttagt tgtgtaat
//
LOCUS       DASJON010000200         4433 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJON010000200 DASJON010000000
VERSION     DASJON010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.204"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..2048)
                     /locus_tag="U3989_002522"
     CDS             complement(543..2048)
                     /locus_tag="U3989_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1715939.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U3989_002523"
     CDS             complement(2045..2731)
                     /locus_tag="U3989_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1715940.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U3989_002524"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U3989_002524"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN1715941.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DASJON010000201         4425 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJON010000201 DASJON010000000
VERSION     DASJON010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.205"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="U3989_002525"
     CDS             690..1658
                     /locus_tag="U3989_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1715942.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="U3989_002526"
     CDS             1669..2484
                     /locus_tag="U3989_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN1715943.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="U3989_002527"
     CDS             2552..3262
                     /locus_tag="U3989_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1715944.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="U3989_002528"
     CDS             3298..>4425
                     /locus_tag="U3989_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1715945.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DASJON010000202         4416 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJON010000202 DASJON010000000
VERSION     DASJON010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.206"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..913
                     /locus_tag="U3989_002529"
     CDS             59..913
                     /locus_tag="U3989_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1715946.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="U3989_002530"
     CDS             1010..3259
                     /locus_tag="U3989_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1715947.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="U3989_002531"
     CDS             3262..>4416
                     /locus_tag="U3989_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1715948.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DASJON010000203         4375 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.207, whole genome shotgun sequence.
ACCESSION   DASJON010000203 DASJON010000000
VERSION     DASJON010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.207"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            153..1577
                     /locus_tag="U3989_002532"
     CDS             153..1577
                     /locus_tag="U3989_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1715949.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1758..2531)
                     /locus_tag="U3989_002533"
     CDS             complement(1758..2531)
                     /locus_tag="U3989_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1715950.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2528..3433)
                     /locus_tag="U3989_002534"
     CDS             complement(2528..3433)
                     /locus_tag="U3989_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1715951.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3587..3907)
                     /locus_tag="U3989_002535"
     CDS             complement(3587..3907)
                     /locus_tag="U3989_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN1715952.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1347 a          776 c          861 g         1391 t
ORIGIN      
        1 gtaatttgga aagggactag gcgctttgct tgtataatga gaaaaaagcc ggtagaatta
       61 tatttctatt aaagttttta ttaattaagt aaaaataaaa aaggtttgtt atactttttg
      121 ataaaagcgg ttttattttg gaggatgctt acatggatac agagcaacag aaaaaaggaa
      181 ttggtttaat tccacttgct gccttagtaa ttggttcagc gattggaggt ggcgtatttg
      241 gtattatgac ggacatctcc ggatcggctg gcggcccagc tattttcagt tgggtactcg
      301 taggaacagc aatgttgatg ctctccttgt cgatcaataa tatcaataaa aaacgtccag
      361 atttaaaagg cgggatcttt agttacgcag aagaaggttt tggccgtttc gctggtttta
      421 tcagcggttg gggttactgg ctcacatgct ggttaggcaa cgtagctttt gcaacattgt
      481 tgatgagcgc attaggatat ttcttcccag tatttgaagg tgggcaaaat ctggcatcga
      541 tcgtgatgag tacagtcttt ttatggggat atgcatggct agtgaatcgg ggagtcgaaa
      601 atgctagttt cgtcaatgct atcgtcacat tctgtaaatt agtgccatta tttttattta
      661 ttgtagtagc tatcagcacg ttttcgatcc aacaattcat tgataacttt tctagcgaca
      721 ttatattgaa tgcgaatgga cagccgatcc cgttttccag tcaagtattg agttgcatca
      781 tggtgatgct ttgggtattt gttggtattg aaggagcctc tgtcgtaggt tcacgagcaa
      841 aaaaacaatc agatgttggg aaagcaacga ttctagggat attcggcttg attattattt
      901 acgtcttggt ttccatgctt ccatatggga caatgacgat gaatcagctg caaacgatca
      961 aggcgcagcc tgctatgggc tatgtgtttg aaatgatggt tggtaaatgg ggagcagtcg
     1021 tgatcaacgg cggtttgatc atttctttag ttggtgtttg gctctcatgg acgatcttac
     1081 cgattgaaac aatgggaaac atggctgaag acggactttt gcctaaagca tggggaaaac
     1141 tcaacaaaaa tggggcacca acttatgcta tcgtattgac gacgttatgt acgaatgtct
     1201 ttttactttc attattattc actgacagtg cttataactt tgcttacaca cttggtacag
     1261 cagctatttt cttcacgtgg cttttcttag gattgtacca aatgaaactt tcttatcagc
     1321 ggaaagaatg ggtccaattt ttagtagggc ttttagcaag tgcctttcaa gtatgggcta
     1381 tgctagtcgt agcctttaat gaagtaatgc ttgttcttgt tcttttcttg ccaggcattt
     1441 atttttacct caaagcacgt aaggagcaaa acgagcctgt acaacatatc cacttagata
     1501 agattttact tataggtgtg acgatggtcg gagtgattgc aatggtttta tttgccactg
     1561 ggatgcttca cgtatagaag tataaaagaa gtataaagag aggttgtgag cctgtcgttt
     1621 attcaaaaac atactaggaa aggagctgtg acacaaatgt tgtcacagct cctttttcag
     1681 aataaacggt gtttaagaga cagccaatcg gcaataaggc tgaacgtctc tttcacagcc
     1741 tcttttttga tgagtcatca tacatttttt cgcttaaata cgagatatcc taatgctaag
     1801 aacaaaacga tgtaagctaa attaccgaat agcatttgat tagtagaaag atgagtcaat
     1861 tgcttcatga tctcttcgtt tccaatttgg ttttgcagat tcaacatact gatcggattc
     1921 catttgatcc actcccattt ttggatgagt gcaataagga taccagatat gattgacgaa
     1981 gcaaaataaa agacgattcc tgctgagatg gatgcgccgg aactattgat aaagcaagca
     2041 atcatcaaca ctaaacttaa aatcagccat aaaccgatat aactacccaa agtataagtg
     2101 aacaagcttt ggatcagtgt ttgaccagta gaaaccttct ccgtgaagga aatcgaaggg
     2161 aaaagaatca gtttcatcaa cacagtgaca ataatagcta aaaggtataa ataaacagaa
     2221 taaataacta atgtgatcca tttgcttact aatatttctc ctcgagaata ctttcgataa
     2281 aggagatttt tcaatgtacc atattgtgct tccatcgaaa tgatcgtgct tgcagctgca
     2341 atcatgataa agacaatcca agatgtggca ctaaaaagct gagcagtcat ttcaactgga
     2401 tcaatgatcg tcgtgtattt tttggctaga agtgctgttc ctatgagcaa aacaactaat
     2461 agaacagata aaacagcagt tgactttttc ttgatcaatt taaataattc ttgttttaca
     2521 agcaatgtca tttttcttct tcccccttta ctgaatccag aatttccaat agagaagatt
     2581 ccaaatcatt cgtcagatgc tggacgtctg tcacatggat attttgtgca gacaatagct
     2641 ggatcacggt tcctaataat tcttcgtttt cttttaaaag cttgatttta tcaggatctt
     2701 ccaaagtata tccagcttct gtcaagatcg ttcgagcagc tagatcattt gttgtagaga
     2761 taacgatgaa atcttgcgct gtttggttca attcagccat tgtacattgt ttgatgatct
     2821 ttccttgatt gatcaggatc agctcatcga ccattttttc aagctcgctg agtaagtgac
     2881 tggagatcaa gaaagaggtg ccctgagtag ccaagtcggt aatgattttt cgcacatctc
     2941 ttgttgcttg tggatccaaa ccgttcatcg gctcatctaa aataacgata gacggatggt
     3001 tcagtaaggc aagagcaatt cccaattttt gtttcattcc caacgagtag gatttcgctt
     3061 ttttgtgaat gtacttttcc atcttgagct gattcacgat agtgtcgata gcagatacat
     3121 cgttggtttc agagaataac ttcagatgat catagcctgt taagaatgga tagatacccg
     3181 ggtattcgat caatgctcct acttgttcca accctttatg actagaggga gaaatctcat
     3241 tttcaccaat acagattgtt ccatctgagt aatggatcag tcctaaaata gctttcatga
     3301 tcgttgtttt tccagcgcca ttaggaccta ctaatccaac gattttgcca ggggataagc
     3361 taaaactgac atcgctgagg gcttgtttgt gaccaaagtt cttggataat tcttggaccg
     3421 aaagactttt caaaaatatt cactcctttt ctatagatag tacaagcata cacctgatga
     3481 gtggagaagt cgctaggtca ttggacttat ttcttatttt ttcataaaaa aattgaatat
     3541 taaataaaaa ggcttatttt ttctgatcag tacttattcc aagtgtctag tggccgctag
     3601 taatcttgat gccaatgata ctgatgagta gcatccccac aaagaaccaa gtggaaagat
     3661 tcagcttatc atgaaacaag accacaccaa ttatgaccga accaactgcg ccaatccctg
     3721 tccagatagg ataagagaga ctcatcggca aacttttgat tgcctttgac aaaaagtaaa
     3781 aactagcaat catgccaatc actgtgtaaa aagaataatt gattttagaa aaaccatcac
     3841 tccatttcat catcgttgac cagaatactt ccaaaattcc tgctatgatt aattccatcc
     3901 atgccatact tacattcctc cttaactata aaaaaataca caaaaagagg aaaatcatcc
     3961 cttcaaagac ctaatggaat gatcatcctc tttatcctac ccggtggcag attgtatatc
     4021 tttttctgta gtatagcaca cctatccaga tatcggcaat ctcatacttt ttagtaaagt
     4081 gtaccaagca ctgacggata tatcgttagc ggaaaagaag atgaatgctt tgcaatttca
     4141 tagaaagaag ttgttattta ttaatcattt aaaaaaaaca gtaactagac cttatttcta
     4201 tatattatcg ggcaataaat taaaaaaagc tttaccttac aataagtaag ataatttttg
     4261 tgataaaaaa ataacgaaaa atatttaaaa atagtgtcta tttcgtgaga aaacaactat
     4321 attttttatg tgttttgctt tataattaaa aaaatattgt cattttatgg aggaa
//
LOCUS       DASJON010000204         4337 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJON010000204 DASJON010000000
VERSION     DASJON010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.208"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..1293
                     /locus_tag="U3989_002536"
     CDS             4..1293
                     /locus_tag="U3989_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1715953.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            1312..1719
                     /locus_tag="U3989_002537"
     CDS             1312..1719
                     /locus_tag="U3989_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1715954.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            1742..2611
                     /locus_tag="U3989_002538"
     CDS             1742..2611
                     /locus_tag="U3989_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN1715955.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            2613..3233
                     /locus_tag="U3989_002539"
     CDS             2613..3233
                     /locus_tag="U3989_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1715956.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            3683..4288
                     /locus_tag="U3989_002540"
     CDS             3683..4288
                     /locus_tag="U3989_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1715957.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         1440 a          739 c          941 g         1217 t
ORIGIN      
        1 aacatgggcg ttatcaattt tatcatcgaa aatattttga ctcaggcatc gatcaccatt
       61 gcattgattg ctatgttggg tctactactg cagaagaaat cagcaggaca agtcatctct
      121 ggcacgttaa agacactcct aggatttcaa gtgttgagtg ccggttcgag catcatcgtt
      181 ggaagtttaa cttatttcgg gaaaatcttt actgaaggat ttcatatgca agggatcatc
      241 ccttctattg aatctatcaa tggtcaagca atgaatgatt tgggtttagg tagagatatt
      301 gcactgacgt tcttagccat ttttgtattc aatattattt tggctcgttt cacaaaatgg
      361 aagtacattt ttttaactgg tcaagcaatt ttatggatgg caacgatgac gacagtcttt
      421 ggctattttg ccggtctgcg agggatcgta ttgatcttgg tcggtggttt tatcggagct
      481 tgtttcgcca ttgctatgcc ggcagtagcg caaccgatca ttcgtaaaat cactggatca
      541 aatgatattg ctttaggtca tttttgtacg atcgggtact tatttgaagc cggtgttgct
      601 aagctttttg gtgagaaagg cgaaaataaa aagtcgatcg aagatatcaa gttgccgaca
      661 catttcgaat ttctgcaaga tacttatttg tctgtcatgg tcgtgatggt cccgctatat
      721 atcatcactg ttttatttgc aggcgaacca ttcgcttctg aattatcagg agatcaaaac
      781 tatatcatgt ttgccttttt acaagccatc caattcgtag tgggtgtgta tgtattgcta
      841 gctggtgtac gtctactgtt aggtgaaatc gtgccggcat ttcgagggat agctatgaaa
      901 ttagttcctg atgcgatccc tgccttagat tgtccagttt tctttcctta tagcccaaat
      961 gctgtcattt taggtttcat tacgacaacg attggtacga ttattgccat gtttactttg
     1021 cccatgttcg gcttagcgat gattttacct ggtatgctaa ccaatttctt tgccggaggg
     1081 acagcaggga tatttggcaa cgcagttggc ggacgccgag gagcgatcat tggcggtatt
     1141 gctcatggtt tctttattac attattgccc gctttgctag taacgatctt taattcgatg
     1201 ggattcaaca atgctacagc aaccgatgta gatacagtag ccgcagcact cttatatgcc
     1261 tggatcttaa gcccagtttt gaaagcattt taattgaaag tagggaaaat gatgtttgga
     1321 ttttttaaga aaaaagaaaa agaaccgaaa aaagttctgg caaaagaaga aaaagcccta
     1381 tcgccagaag cgaccgaaaa gattcaatca gagattacac aattgaaaca agagatttcc
     1441 actacccaag ataagcataa attagcgaaa ctttatgaac aagttggact taaattttct
     1501 gaattgtatg tgaacgatca agccatccaa tatttagaaa agagtttgga aaataaacag
     1561 acgatcggag atgggtataa aaaactgatg agcctctata accagaagcg tgcagatgct
     1621 gctcgtgctg gtgatgatca agggatcgat tactacatgg gcaaaatgga tgagatgcga
     1681 caaattgcaa aacaagtaac gatcaaagga aacaaataaa aagataaggt ggcaataaaa
     1741 gatgtataca acattgaaag aagtaacaaa aacagcagaa gagttgaatt ttacagttgg
     1801 agcttttaat acgcataacc tagaaatgct tcctgctatg ctaggcgcag caaaagaaat
     1861 gggttcgccg atcattattc aaacaagtgt cgataccgct aaatatatcg gcatgcatgt
     1921 gttagtcaat gtaatgacag ctattgcaga agaagaaatg gttgatgcag tcttgcactt
     1981 agaccacgcc cgagactttg atgatatcaa agaagcaatc gacagcggat atacttctgt
     2041 catgtatgat gggtcccatc tgcctttcaa agaaaatatc ctgaagacaa aagcagtagt
     2101 agaatatgcc catgcgcacg gtgtttccgt agaaggagaa cttggaacaa tcggcggtac
     2161 agaagaagga atccatgtcg ctgaaaatga caaggtatat acaagaccgg aagacgcagt
     2221 gaaatttgta gaagctacag gcgtggatgc attagctatt gctatcggta cgaatcatgg
     2281 acaattcaaa tcaaaaacag aagtaaacat tcccttgctg aaagaaatcg atgcagtagt
     2341 tgatgtcccc ctagttatcc atggaggaac aggtgtgaaa gaagaagatt atcctgaatt
     2401 gatcaataac gggatccgca aattcaatgt tggaacggag cttttagtca actggacgaa
     2461 agtagctaaa gaaaccttta gtgaaacaga aatcaacaag tctcttcgcc ataatgtgat
     2521 tccagcaaat gaagcagtaa aagaaattgt caagcataag attggactgt ttatgaactt
     2581 ggaaagtcca attaacttag gaaataaatg agatgaaaaa gtatttcatt cttctggctg
     2641 gtagtcctgg tacaggaaaa acatatctta tgaataaact acgggaacga tttccagata
     2701 tgtatgccat cacgctcgat gaaatcaagg aatactatgc agaatccatc gggtttaaca
     2761 atttggaaga acgggcggaa caagaaagga cgaaagttta tcctttctat tacaaagcac
     2821 ttgatttgta tatggaagca ggaaagaaag tgattgtgtc cgagtatcct tttagtgata
     2881 agcaaaaagc acgactccaa gaattagcag atagatacgg atatcaggtg atcacgattc
     2941 gcctaacagc agactttgaa gtgttatggc aacgtcggta caaacgtgat cgtgaatcag
     3001 atcgtcattt gagctatatc atggatcatt atcactatgg tgacgtattg gaagatcgaa
     3061 cgaaagggac caatcatatt acgaaagaag cattcaaaaa gatcatcgcc gaccgaaaat
     3121 atgacaaatt ccagttagga acactatatg aatttgatgt aacagactat actcaagtgg
     3181 attacactcc actattggat aaattagaaa aagaaatcaa tgacaataag taacaaagaa
     3241 atggcttaat atcgaaaaat tggcttttga acgctgttta ccgagaggtc gtgagcctgt
     3301 cgttcagctc cgagtcacaa ggagcaattt caaaaaaaca gcttctcatg tttgatgaat
     3361 attgtgactt gtgaccgagg agttggtagg agaacaccgt ttagtgggga ggttgtgaaa
     3421 aagctgtcct gcctcaatta ataagaacag ccagacaaga atagctcgtc atatttttcg
     3481 tttggtgggg cttattaccg cagatgcaag cttttgaacg ccatttattc acagccccag
     3541 agaagtcgaa tgtcttttgt tctcgagttt tttaacattc ttttatttat tgcagatttc
     3601 attatattta tttgccgtaa ttaaaccagt cttctatact tgattacata gtcatacatt
     3661 tgctaaaaaa gaagggaaga gaatgaacaa gaagttggct caatacaaaa gactactcgc
     3721 actttctcaa gctggtctat attatggaaa agatgtattt gataaagagc gttttgagga
     3781 gatccggacg atttcattgg aactgatcag tgaaatagga aaagaaactt tcgaagaaat
     3841 aaaagcgctc acaacgattg gagaaggaca tccaacacca aaagtagatg ttcgtgccta
     3901 tatcaaaaaa gatggcaagg tcctactaat cgaagataaa cggacgaaag aatggtcact
     3961 tcctggtgga tttgctgaaa taggattgtc tcctgaagaa aatgtgcgta aagaagtcta
     4021 tgaagaaaca ggattaacag tagaaactac tcagttgcga gcagtttttg atacgaataa
     4081 acaaaaagat attccacagc tttttcagta ttataaactt gtttttgctt gtgcgatcca
     4141 cggtgaagag gccaagttta ttgagaataa tgaaacttcc aacatgggat tttttagtat
     4201 agaagagttg ccaaagctat cagaaaagcg aacaacgaag aaacagttac ttattttaga
     4261 aaacaaaaac cagatctatt gtgattgagc tttttcgtaa aagaagaaaa gaatatactt
     4321 tactagttat taaaaaa
//
LOCUS       DASJON010000205         4288 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJON010000205 DASJON010000000
VERSION     DASJON010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.209"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..288
                     /locus_tag="U3989_002541"
     CDS             <1..288
                     /locus_tag="U3989_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715958.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="U3989_002542"
     CDS             347..787
                     /locus_tag="U3989_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715959.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="U3989_002543"
     CDS             799..1467
                     /locus_tag="U3989_002543"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1715960.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="U3989_002544"
     CDS             1531..2154
                     /locus_tag="U3989_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN1715961.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="U3989_002545"
     CDS             2210..2596
                     /locus_tag="U3989_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN1715962.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="U3989_002546"
     CDS             2809..4200
                     /locus_tag="U3989_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN1715963.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1428 a          714 c          913 g         1233 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttc
//
LOCUS       DASJON010000206         4255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJON010000206 DASJON010000000
VERSION     DASJON010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.211"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(108..638)
                     /locus_tag="U3989_002547"
     CDS             complement(108..638)
                     /locus_tag="U3989_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN1715964.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            743..1294
                     /locus_tag="U3989_002548"
     CDS             743..1294
                     /locus_tag="U3989_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1715965.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1447..1920)
                     /locus_tag="U3989_002549"
     CDS             complement(1447..1920)
                     /locus_tag="U3989_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1715966.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2058..3530
                     /locus_tag="U3989_002550"
     CDS             2058..3530
                     /locus_tag="U3989_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1715967.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3527..4237
                     /locus_tag="U3989_002551"
     CDS             3527..4237
                     /locus_tag="U3989_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN1715968.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1335 a          767 c          824 g         1329 t
ORIGIN      
        1 ccctcggtat tagctcaaaa aacggcaaaa catgaggaac tatttttccg tttctttctc
       61 taatactcag gtcagaacgc tgatttcaca acctctcttt cacaacctta ttttactcgt
      121 ttataatttt catcgaagta ttttccgcta cggatatcat ctggcattcc atcgtatggt
      181 tcttcgctaa taacatcttc attgtctata cctgcatcac ccatataagt gatcgtagca
      241 aattcaggaa caagtaattt tggataagtt tcatattttt cacgagctgc ttccatttct
      301 gcgatatgtt cgttttggaa agtaacagtg atttctggat gttcttcaac ccatttaggg
      361 aaaaagatca ttccgtgaag tttcttttca tttggcataa agtccaatgc cacgtctgtc
      421 cctgttggct ctgtccaagt gattttgaat acgccgtccg tcaatttaac gatatcagct
      481 tcttggtctt ttacccaacg gcctttgacc attccgccat ggatacggta atcaacagta
      541 tggtcatttt tggcatacca ttcatattcc caaccattgt catacgtata gataaagtga
      601 gttccaataa aatcttccaa ttctttaaat tctttcatgt ttaagcatct ccttataaat
      661 taaaacatgt aataaactac atgttttcga ttactcattt atgatagaat ggatttaaag
      721 atttgtcaag gagggaaaac tgatggcaag aaaaaataca cttcgatata ttttgttagg
      781 gttattaagt aagaagaaga tgacaggata tgaattgaat caatcgttca aaaatgaaat
      841 aggagaattt tggcaagcaa aacatagtca gatttatcct gaacttgcaa aaatggaaga
      901 gcaaggaatc attcagcatc aagtagagat cacaggtgaa aaattggaaa agaaagttta
      961 tgaattaaca gaagaaggga aggaacaatt ggcagagtgg attcatacac ccaccaatga
     1021 actaccggtt aatcgagacg aatttgttct gaaattgtat ttcgtcaaag acgtgaatga
     1081 tccggcatta gtagaaatta ttcagcagca gcgtgacctt catgaagaaa aacgacttca
     1141 tctattagag cgaaaaaaaa cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg
     1201 acactacctg attttagcgc atgcgatcaa tcgggaaaca gaatatgcaa attggctaac
     1261 agatgtatta gaagaaagta accgaaaaaa ataaaaaaat atccgaacag tgatggagaa
     1321 ttcctttcta tatgaaagaa acgcagatca ccgttcggat attcttatgt tcaaacaggc
     1381 cttttttcaa gtatttatgt agcatctact tattaaaaag cttggtactt aggagattag
     1441 tggcgtttag agttctttta acaactgttc gtagtcagct ttttcatcga caggaaggag
     1501 cttgtaagtt aattcgtctg tctcgttgtc atgaatgatg tacatcttag cattctcttc
     1561 tttgaagtta ccgatttgta aaatcgtata gcgttcgtcg atcaattcga tatctttgat
     1621 tgcttttgaa aaaactttac ggacatggtg cattttcttt tggcggattt ctgccttttc
     1681 tgcagctttt gacaaagtaa atccgtcatc taaatatttt ttgatcagtt ctacttttat
     1741 gactgtaggt aaacgatatt ttctaggaca attcgcatca gcttcgatcg aacgaatgaa
     1801 tcccttttgt tcccaatacc ttaattgacg tggagacaca cctacgatat cacttaattc
     1861 actgatgcca acaaccaatt gatcattttc caacaaacgt ttaagggaag ccccaaccat
     1921 ttttgtcact ccttttgtcc tcttgcttcc cttaattata tccgtattaa cttatgtgtc
     1981 aagtaggttg acaaatagcg aatttaggac taaagtattc tagtagtttc agaaaaaatt
     2041 cagaaagaag gagaaatatg agtaacaacc aacccgtaga tatttacgga aaaccatata
     2101 atcggacact tttagttgtt gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga
     2161 ccttattgac gacagctttt cctacattga tgaaagcttt cgatatttca gcgtctgatg
     2221 tgcaatggtt aacgactggt ttcttgctag tcaacgggat catgatccca ataacagcat
     2281 ggctgatcaa taaattcagt tcaagaaatt tatatctttc tgccatgacg atctttttga
     2341 tcggaacgat tacttgcttt acagcaccta attttagtac attactaatc ggtcgtttga
     2401 tccaagcttg cggtgtggga gtatcgatgc cattattgca aaatatcatg ctatctattt
     2461 tcccgccaga aaaaagagga tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc
     2521 ctgctttagg ccctacatta tctggttata tcattgataa ctaccattgg cgtgacttgt
     2581 ttggtatggt actgccaatc gttgttcttg tcttggtttt agcattcttc ctaatgaaaa
     2641 gtgttattcc attgtcaaat cctagcattg atattttatc cgctatttta tcaacgattg
     2701 gattcggtag tttactttac ggattttcta gtgtaggaga tgacggctgg ggaagcttga
     2761 aagtaattgg tttcttagtt gttggtgtga ttttcatcgc cttgttctca tggcgtcaat
     2821 tacatttgga acatccattc ttggaacttc gtgtattcaa atcatctaca ttcacgattg
     2881 cagctgtttt agctggtgta acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat
     2941 tgtatattca aaatattcgt ggagaatcag cattccattc tggcttgatg ttgttgccgg
     3001 gtgcattgat catgggggct atgatgcctg tgacgggtgc gattttcgat aaatacggcg
     3061 cgaaacgttt agccatcaca gggatgttca ttttgacagc ggcaactgca ccatttgctt
     3121 tcttgacaaa aacgactcca gttttatata tcgtgatcct ttatgcgatc cgtatgtttg
     3181 ggatctccat ggtcatgatg ccggtaacga cttctggtat gaatgcattg ccaaataact
     3241 tgatcagtca tgggacagcg gtaaataata cattccgtca aatcgcaagc tcgattggta
     3301 cagctatttt gatcagtgtg ttgacgaatg tgacaaaagc aaatatccca agcaaatctg
     3361 tattggataa tacgccatta gcttataaag atcaggcaat caatgccaca ctgtcaggtt
     3421 accatgccgc gtttatcgta gcgatcatct ttgggattct tggctttatt attgcctttt
     3481 tattgaagaa aaaagaacct gttctaatga aagcggggga taatgcatga ttattggagt
     3541 tttgattgtc agcttgcttg cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca
     3601 aaccttttta tctttgattt tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc
     3661 taatttgtcc aatcactttg gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt
     3721 ttccagtgct gattcccaag gagcagatat gcttttgtat aaagctttag ggaatggaaa
     3781 agaaaaagtg tatctttacc gaacaaatga aaaacaagaa aaaccaaaag caactggtac
     3841 ggataacgaa acaaataaag tagaaaagac agacggcgat gctgaaaaag tgacgaaaac
     3901 tacttattgg gaatacaaaa acgacatgta taaattctgg ttcaatattg ctgataacaa
     3961 tcacgaatat gacagccgag tgaatacatt caaaatccca gagacatggg tagaattaag
     4021 tacggaccaa gcagccaagt tagctgaatt agtgaagaaa cagcaaagta cgatggaaag
     4081 tgaagcaaaa gcttatgttc aagacggtat ggtaaaagca atgactgaaa atccaaaaat
     4141 gagcaaagca gagcaagaac aacgtacgaa agccttagct gctgaattcc aacaacaagc
     4201 attcgcaaaa ttagtgaaag aagcaaaagg cgaataatat tttgaacacc atgta
//
LOCUS       DASJON010000207         4251 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJON010000207 DASJON010000000
VERSION     DASJON010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.212"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            832..1755
                     /locus_tag="U3989_002552"
     CDS             832..1755
                     /locus_tag="U3989_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1715969.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1771..2310
                     /locus_tag="U3989_002553"
     CDS             1771..2310
                     /locus_tag="U3989_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1715970.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            2991..3164
                     /locus_tag="U3989_002554"
     CDS             2991..3164
                     /locus_tag="U3989_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715971.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            3569..4003
                     /locus_tag="U3989_002555"
     CDS             3569..4003
                     /locus_tag="U3989_002555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715972.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1611 a          632 c          698 g         1310 t
ORIGIN      
        1 ttatgagcaa attaatgcaa aatttggcta gcaaaaaagc taaaaaatat atgctaataa
       61 tcgagcgaaa taaacacttt ttcaagtgag cgaatccgcc atgacaaagc tgaagcagtt
      121 tatttcagct ttgtcatggc gtgtgccgac ttttttaaaa gtctaggcac acgagcaaat
      181 atctttatca agctctaatt tgcgttttaa gacgtttgaa acgcctaagt atataaatat
      241 ataagaatag gtttaagagt gcttaaatcg aattttagag ggcatttaga caattttagg
      301 gttgttaggg cttgccctga ccgcctgtaa ggcgttttaa gaacaagata agggatttat
      361 actaggactg tcttaaaatg tcttaaacga acgctcagag cttctcaaag ggttccttat
      421 gaacggcttt tagcaaagga gccggaggcg actgcgcagt ataatgtgag agcagagcga
      481 tcacaccttt ttagcgaacc gtagggagca aggtttggaa ttggcagttt tgataaattc
      541 gcgaacctgt ttagagaaaa aaggagtgat tttttaggcg ggaaaattag cctcccctct
      601 ttttatatct ctttttatat ctctttttat atctctttta tgtcggctgt gtccttactc
      661 tcccaagcaa ttcagagttt tttcaagtag gttttctacg cttttcaagt aggttttcta
      721 cgcttttcaa gtaggttttc tacgcttttc aagtaggttt tccgacatta ttgagtattt
      781 ttataaagta tgttactttt atttcatact ttataaaagt aggagaagct tatggcaaat
      841 gaaattgtaa aatatgaaaa tagattaaat catattcctc tgagaaaatt taatagtcgt
      901 gaaatgaact tatttttttc aattgcttca cgtgtacgtg aaaaaggtgt agatgagatt
      961 attttttcat ttcacgattt gaaaaaacta agcggttata cagaacgtgg ggaacaattt
     1021 ataaaagatc ttagtagcac gtatgacaaa ttattaagta ttaatgcttg gaatgatgat
     1081 ggaaatactt taactaaatt tgttgctttc acaaaatata gcattatacg agataaacaa
     1141 cttgttataa tttcagttaa tccagaattt aaaggacttt ttaatcaatt aaagaactgg
     1201 acaagattta atctagagca atttactaat ttggatagca catattcaaa aactatgttt
     1261 cgattattaa aacaatatcg aactcaaggc tggactgaat tttccaaaga aaaatttttt
     1321 gatttactag atattcctaa aagttattgg aatagtccgt caaatattga caaatttatc
     1381 ttaaggccaa ttaaagaaga actcactccc ttatttaaag gattaactat tcgtaaaaaa
     1441 tatggtaaag gtcgaggtaa acctgttatc ggttatcgtt ttacttggaa agctgaaatt
     1501 aaccatgctg acgatttttc caaagggaaa caagaagatt tacgtataaa attatttaat
     1561 attgatcaca atggagaatt aacacgagaa gaaaaatggc gcgctaaaga tcgaatacta
     1621 aatcttccct taggcactca tgaagctgat tttaaacaac aacaacaaac tgaagaaaaa
     1681 caggcagaaa aacgagaact ttcaaacaaa ctcaaacaag atttactagg aaatttacaa
     1741 aacttattcg attaagctta aggagtattt atggcagata aaacaatcaa agaacttgcg
     1801 gaagaattag gagttagtaa aacagcaatt aacaaaaaag tttctgacaa aaatcgaaaa
     1861 ctttggtttg caaaaaacgg aaacaagttt gtgattaacg aaaccggtca aaaagctata
     1921 aaatcaatgt ttttagtcga taacgcaaac ttaaatcgaa aactagttgg cgaaaaatca
     1981 aaaaccaaaa accataaaaa caccaaccaa gtttccgaat taaaactcct ttctttccta
     2041 gaaaaacagt taaatcgaaa agatatacag ctacaagaaa aagataatca aataaaacaa
     2101 atgcaaaaat tactcgacca gcaacaacaa ctcactctac aagcgaacca acaaattgag
     2161 aacctacaag aacagctcca tttaacttat acagaagaaa ctccaacaac cgaatcaact
     2221 gccttttctg aaaaaaaagc agacaataca caaccactta ttgaaaaaaa gtggtggcaa
     2281 atttggaaat ctaatagaac caatctctaa aattttctag aagttagttc ggatagtaga
     2341 ggaaaaagtt gtactacctg atggggagat cactcaaatt tttatgagaa agaaaaaata
     2401 gtttacaact gtaaacaatc aatgtttata gttgtaaact atcaaactat atggtgactt
     2461 attgcttaaa ataggttgaa atctcactca atttttggtg gaaaaacaaa ataaattcta
     2521 atcgattttg ttgaaaaagt cataaaaaaa caaatgtgat tttaacaaaa tttcaaaaaa
     2581 atcgtttgaa atttcgttta ttttttttga gaattttgat catcgtaggg tcattgtaga
     2641 atcattatga gatcatcgtg ggatcatcga ataaagtatg ggaaaggctt gcggtgcttg
     2701 gtattaaggg cattctccgc ctcccaggtt cttgaccatt ttctcttata agtgggattt
     2761 ttaccacgtt gagagaaaat ggtcaagaac ctgggaggcg gagaaaacat accaagcacc
     2821 acaagcaaaa aaagttagag taacttccaa aaaagaaaaa ttttttagaa caacttttga
     2881 aaatttaatt ttgatttata tccgttcaat ttaaacattt gataaactga aggctagtat
     2941 ttaaaatgtg tatacaagtg gtagcgttaa aaaaagtgga ggtataacct atgttaaaaa
     3001 aaactgtgtt ggtactgggt tctatttctc taggaatatt aggttttagc cccatgctat
     3061 tatccgtaat tggaaaaaac aattccaaca gagaacttaa attgaattca aattattaca
     3121 acagtcctaa cgaagaattg tttgtaaaag aaacggataa ataataaaaa agactgctct
     3181 tccgagcagc cagttaaaaa ataatgagtg tttattcttg aaaaagcaac gcgcttataa
     3241 agcgctacca aaaatttatc atattttatg aaaaataaca atccagtaaa aaatgactgg
     3301 taaaaattca caaagaaacg gaaagaagca actgcagaaa atcaagcctt aaaaagagct
     3361 ttttacctag accaattaaa aatcacaaag tgagcccctg tatcggttga tacaagggct
     3421 acttggcata ctgtacatta tgtctactaa cctttaaaat aacacgtttt ataaaataaa
     3481 ccctcttgac aatatttaat tttctgatta ttctaaatat atacagaaag cgctttccta
     3541 ggattttata tctaaggagg aagtaacaat gaaaaataaa attattaaaa ttattacttg
     3601 tctatttttg ttcggtatga ttgttggagc aagtactccg ttaaatctgt ttccttttag
     3661 tgctagaggt ataaaagtgg aagctgcaag ttcacgatat aatcataatc atcgtggatt
     3721 tacttgctat aaaatgaact attatgttac taataaaatg tgtagagatt tgaaaaaaaa
     3781 ttataataag ctaaagactc ctgctcaaat tgcatctttt attcctattg gtggagttgg
     3841 aacatgggtt attacaaatg cttttagtag tgctgttgat gcaatgaatg tctttgtaag
     3901 agctgcaaat caaggaaaag gtgtacaatt aacatataat gctcacttta gcaatacaac
     3961 atcatatcaa tacaatgatt acgctcgtta tgtaataaaa taatcagaaa gtgaaactat
     4021 ctcatgaata aaaagatttt agctgtaatt atttcaataa tagttttaat cactatgttc
     4081 ttcatattta ggctaatttt caatataagc ttacaaaaaa gtattcttta tctaattcct
     4141 atagcattta tcttagcaat ttttagaagt atttacggaa ataaaaactc taaatagcta
     4201 aaaataaatc atactaaatt ttacaaaaag tggctagcat tttctagcca c
//
LOCUS       DASJON010000208         4230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJON010000208 DASJON010000000
VERSION     DASJON010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.213"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..873)
                     /locus_tag="U3989_002556"
     CDS             complement(<1..873)
                     /locus_tag="U3989_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1715973.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEK"
     gene            complement(923..2419)
                     /locus_tag="U3989_002557"
     CDS             complement(923..2419)
                     /locus_tag="U3989_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1715974.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2613..>4230)
                     /locus_tag="U3989_002558"
     CDS             complement(2613..>4230)
                     /locus_tag="U3989_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1715975.1"
                     /translation="QKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLGID
                     AIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVNHT
                     SDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFLHL
                     YSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPKLH
                     EYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQEGK
                     DKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKESAK
                     MFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEILE
                     SIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMYKK
                     LIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPREAL
                     RVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         1234 a          885 c          674 g         1437 t
ORIGIN      
        1 tttttcatca ttaatcatca aataatttaa ctctaatagt cgattttcaa ttgcttgttt
       61 ttcttgattg gaaaaagaat agggtagttc tgatgataac ttttttgtga gggtctctac
      121 catcggaata aataatgtat acctgtttgt tttttctaaa agttcttctc ttcgaagaat
      181 ggtcaaaacg agataccgag aaaatcgtat gaagtcctct cctgaaacaa tataattata
      241 tgatataaga acctctttta atgtcttttg gacagtcggc atgatattgg aaaaaattgt
      301 actgatttgt tgaggaaacc ttgcgtgttg gatcaccgtt tctgttccaa caagagataa
      361 gaaaattctt tttgaatttt cttcagcaat gatttttagt ccttttgtcg acgaaacttc
      421 cagttctaat ttaggattgc gctcgatcca acgcaaaatc gtttttattt gtaatgatac
      481 ggctgttttt gaaagaaaga aaatgtctgc tatcttttgc atactgacat accctttttg
      541 aaacaataag tacgtcacaa tagttgaagc tcggaaatcc atgctggaat agattttatg
      601 tttcgtcacc tcttgtatga tttgctgttt agtttcagat tcaattgatt ctaaaaaata
      661 acctgaacgt ttatttgact ggataatgat tttcttctcg tagatagcaa gcatattttt
      721 gatttcagtt tggatcgttt tcttgttggt acctaaagct tcagctaatt cttcgcttgt
      781 tatccaatga ttcttatttc ttaatactac aagaatttgg agttgtcgtt tatttaagtc
      841 catttgctta cacctcctct ttgttataaa catacaggaa aaacctctca aatgacaata
      901 gtcacttgca gaggtttaaa aatcatttga agaaagatct atattcaagg tttttaccat
      961 tagactggat aatttgttta taatattcaa aactatcttt tttcaatctc ctaaagcttc
     1021 cgctcccatc attttgtcta tccacataaa taaagccata tcgcttcctc atttcccctg
     1081 ttcctgccga tacgatatcg attggtcccc accataagta accaatgatt tggcaaccat
     1141 cttttattgc ctcagcaatt tcctgaagat gtatttttag atactcacaa cgttttggat
     1201 cgtggattcg attcaattca tctggtgctt catctagtcc aatgccattt tcaactatga
     1261 ataatggtaa ttcataacgg tctgtcaatt cattcatgac ataacgaaac cctagaggat
     1321 caattggcca actccaaggt tcgggagatt tttccttcaa aaagggatta tcgaaaccat
     1381 ttgctccgcc agtatctacg cgcatttctg cttctttttt atagacagcg ctacgataat
     1441 aactaaatgc aatataatct gccgtgttag cttgcaagca ctgttcgtct ccatctaaca
     1501 ttactggtgc acagtcttct tccttccatg tcctgtaaat ataagctgga taatgccctc
     1561 ggcacatgac atcggagaaa aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat
     1621 tctctggatc acagtcatat gcataagtcg ctaatgcaga aaagctacac ataataccca
     1681 tcttagcatt tggatcgatt ttctttaata attgagtagc acgggcgttc gctagaaaca
     1741 tatgatgaca ggcttgataa atcacttttt ttgttagtgt agcaatgggt tcaagtttat
     1801 ctctagcagg gaatgtaacc aatccagcag ctgcataagg catacgaaaa gcattattga
     1861 tctcattaaa ggtcatccaa tatttcactt tcccttggta gcgttcaaag acggtctgcg
     1921 cataaaattc aaaaaagtcg attaattttc gattttgcca ccccccatac tctcctacca
     1981 atgctaaggg ggtttcataa tgactaagag taattactgg ctcgatatca tatcgattta
     2041 atgtattgat tagtctgtca taaaatacga gaccagcctc atttggcttc tgttcgtctc
     2101 ctcttggaaa tattcgtgcc catgagatgc ttgttcgaaa agcttttaaa cctaactcct
     2161 tcagtaaacg aatatcctct tcaaatcgat gataaaaatc aatcgcttca tgagaaagat
     2221 aatggagtga ttcgtctaaa gcaatctccc aaatcttttt ttcttcattc cactgaatac
     2281 taggatgctg gttcatgatc ccgataagcg tatctgtcac attaggtaat ttgccgtctt
     2341 ccaaccatgc accttcacat tgattagcag ctactgctcc gccccataaa aaattttctg
     2401 gaaatatttc ctgatccata actatctctc ctcgctatca acaatttctt acttgaagta
     2461 tagctcgatt catcaaaaca agatattcta actatttcgg cagaagagaa gaaaaaaatt
     2521 gaatgctact cttcattttt aatatttata tataacaaat acataagacg atccccgaaa
     2581 gaaaacactc gttaaccatc taagcaaacg atttatttta cttccaacac aaatgcttca
     2641 taaggtcgta aatcaacttt gccgtctggt aactgtttca gaggataatt tgacacaata
     2701 acccttaaag cttcacgtgg aatagtgaac gtctgctttt cactggacat attacaaata
     2761 acgagccatt gctggtcttc atatatgcga gaataggcaa agacatgctc aggcgtcttt
     2821 tccactaatt catagtctcc ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat
     2881 ttcttgtaca tataaaaaac agaatcttga tctgctaacg cttcttctgc attgatttct
     2941 ttataatttg gattgactcg aagccatgga attccagtag taaacccagc atgttcacgg
     3001 gcgttccatt gcataggcgt cctagcattg tctcttcctt tcgccttgat tgattccaag
     3061 atttcctctt ttgtaaatcc agaagaaatc ctttcgttat acatattgat tgtctcaata
     3121 tcttttgctt ccgcgatatc atcgattggg caattcgtca tgcctaactc ttctccttga
     3181 tagatataag gggtgccttt catcatatgg agcaagatag caaacatctt ggcactttct
     3241 tttcgataca catcgtcatt tccccaacga gaaacaattc gcggaagatc gtgattattc
     3301 caaaataaac tattccatcc ttcatcacct aaagatgttt gccatttgga taatactttt
     3361 ttcagttcag agatactcaa gggtttcaaa tcccatttgt ctttgccttc ttgctgatct
     3421 aatcctatgt gttcgaattg gaagaccatg gataattcat ttcttttagg attggaataa
     3481 agtttagcga tttcggtagt tgctccccat gtttcgccaa cagtcatcag atttttgtca
     3541 ccaaaagttg cttgattcat ttcctgaaga tactcatgca gtttaggtcc attacttgtg
     3601 atttctttat ccggttcttt gccaatcata tcgatcacgt ccatacgaaa gcccccaata
     3661 cctttatcga tccagaaatt catcatttga taaatctctt ggcgaacctg tttatttttc
     3721 caattcaaat caggttgacg cttgctatac aagtgcagaa aatattgccc cgttttttca
     3781 tccaactgcc aagcagaacc actgaatatt gagcgcaatc tattaggctc ttttccattg
     3841 accggatcac gccaaatata atagtcccga taaggatttt ctttattttt acgggcctca
     3901 atgaaccacg gatgttcatc agaagtatga ttcaccacca gatccatgat gatacggata
     3961 tttcttttct ttgcttcttc aatcagttct tccatttctt ccatcgtacc gaattctgcc
     4021 atgattgctt gataattact gatatcatac ccattgtcat cattcggaga ctcgtatact
     4081 ggagaaagcc aaattgcatc aatacctaaa tacgccagat aatccaacct ggaaataatc
     4141 ccccgcaaat ctccaatacc atcttgattt gaatcttgaa aacttcttgg atagatttga
     4201 taaacaactg aatggtgcca ccatttttgt
//
LOCUS       DASJON010000209         4185 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJON010000209 DASJON010000000
VERSION     DASJON010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.214"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(3..1223)
                     /locus_tag="U3989_002559"
     CDS             complement(3..1223)
                     /locus_tag="U3989_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN1715976.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1854)
                     /locus_tag="U3989_002560"
     CDS             complement(1243..1854)
                     /locus_tag="U3989_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1715977.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="U3989_002561"
     CDS             complement(1897..2829)
                     /locus_tag="U3989_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1715978.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3250..4110)
                     /locus_tag="U3989_002562"
     CDS             complement(3250..4110)
                     /locus_tag="U3989_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1715979.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1187 a          847 c          688 g         1463 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgtttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga taattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tagttaatca atagtatcat ttttttcata tgatttaatt catcaattaa
     3301 ttttttgttt ttattaattt tagcaaaagc tagtgcatct tgaatcaatt cgagcacttc
     3361 tgccttcgac ttatttttag cctgtgcgtt cttcgctaat tgggcagcag ctcgtgctaa
     3421 gtaataagtc aaatggttat ccgaacaaat atcgattgcg taatttagta gtgcatcact
     3481 tgtttccaaa tcaccgtctt gcgcaaaaaa aacaccacaa tgaaagacga tatttaacac
     3541 ccgccagata tcttctgttg tacgaatcgg atacgtataa attttttcaa acactcgatt
     3601 gaaataaact tctgcttttt ccaactcttt ttctctagca tagaccattc caatacctgt
     3661 atatgccaat aaacgaaaca acgtgttggc tgtttctgcc atcaacaact gatcaaaatg
     3721 gaacaacaca tccgtaattg gtgcattcgt aaaaatcatt tcaaatcctt ttagatagta
     3781 gtaacgtgca atcagctcag actcgtcaat taccttggga tcaatggctg ctaaaatctg
     3841 tgtcaattgt tcatattcac tcgtaatcag tgaaaactct gctttttcta gctgttcatc
     3901 aagttgtgta ttgatcacgt tcatcgaaga aaaaagttca actaacggaa gattgattcg
     3961 attacataat ttgattaaaa tttttaacga agggacttga ccattgttct caaatttact
     4021 taatgttgct tgtgtacaaa ttccttcaga cacttctttt tgagataagt tggcagcctt
     4081 tcttgccgta ataaattttt caatgttcat tattttctct ccaatcagac ctcacacgaa
     4141 aatcatttct gtctcgattt tagcatgttt tctttgggaa tgctt
//
LOCUS       DASJON010000210         4070 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJON010000210 DASJON010000000
VERSION     DASJON010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.215"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            598..1287
                     /locus_tag="U3989_002563"
     CDS             598..1287
                     /locus_tag="U3989_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1715980.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="U3989_002564"
     CDS             1274..2482
                     /locus_tag="U3989_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1715981.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="U3989_002565"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="U3989_002565"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN1715982.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1320 a          704 c          809 g         1237 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta
//
LOCUS       DASJON010000211         4039 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJON010000211 DASJON010000000
VERSION     DASJON010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.216"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..740
                     /locus_tag="U3989_002566"
     CDS             <1..740
                     /locus_tag="U3989_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN1715983.1"
                     /translation="IQNKIDFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKI
                     DMYGLPVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEM
                     FGIPVGYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKH
                     IRNTEIFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAP
                     KFLYQYIGKKVKRPISADSVLLEEDVE"
     gene            737..1885
                     /gene="neuC"
                     /locus_tag="U3989_002567"
     CDS             737..1885
                     /gene="neuC"
                     /locus_tag="U3989_002567"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN1715984.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1858..2535
                     /locus_tag="U3989_002568"
     CDS             1858..2535
                     /locus_tag="U3989_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1715985.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2525..3667
                     /locus_tag="U3989_002569"
     CDS             2525..3667
                     /locus_tag="U3989_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN1715986.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            3680..>4039
                     /locus_tag="U3989_002570"
     CDS             3680..>4039
                     /locus_tag="U3989_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1715987.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFL"
BASE COUNT         1467 a          523 c          800 g         1249 t
ORIGIN      
        1 gtatacaaaa taaaattgat tttttgtcta ctccttttga tgaagaaagt gcagactttc
       61 taaaacaaat tggtgtacaa ggatttaaaa ttggctcagg agatttaaca aatttacctt
      121 ttcttagaaa aatagatatg tatgggttgc cagttttatt atctacaggt atggctgata
      181 ttcatgaggt cagtgaggct gtagattgtt tcatagatag tccagttact attttacatt
      241 gtacttcaaa ttatccggct agttctgaag atattaattt attggcgatt aacacattaa
      301 aagaaatgtt cgggataccc gttgggtact ccgatcattc attgggatat gatgttggta
      361 tttgtgctgt tgcgatgggt gctaaggtaa ttgagaaaca ttttaccttg gataaggaac
      421 tacctggtcc agatcataaa gcttctttag atcctcagga atttgcagat tttgtaaagc
      481 atattagaaa cactgaaata tttttaggtg atggtgttaa gcgagcaatg ccttctgaac
      541 ttagtacaaa agaagtatca agaaaaagcc tagtggtcac tgagaattta aatgtgggag
      601 atattcttac agagaaatct cttacaataa aacgtcctgg aaatggaata gctcctaagt
      661 ttttatatca atatattggc aaaaaagtta aaagaccaat aagtgcagat agtgttctgc
      721 ttgaggaaga tgttgaatga aaaaaatact tatagtaagt ggaacacgag ctgattatgg
      781 aatatataaa cctgttgctg agaatttact acaagataat tgggatgtat catttgttgt
      841 ttctggaatg catttatccc ataaatatgg atttactaaa gaattgatct tagctgacgg
      901 atttagaatt agtggagaag ttcaaagttt gtttttagag aacaataagg gaaacatggc
      961 tcggtctgtg gcattagaga taaatggttt cacaaatata tttgaacaaa ctagccctga
     1021 ttttgtatta ttactagggg atagaggaga aatgttggca gctgcgatag cttgtttgta
     1081 tcttggaatc aaaacagcac atattcacgg aggcgaagtg tctggtactg tagatgaatc
     1141 aataagacat gccataacga aaatagcatc tgttcatttt gctgctacgg aaaaaagcaa
     1201 agagcgttta ataaaaatgg gggaagattc ttggagagtt tttcccgtcg gagctcctag
     1261 aatagatact attttaaata atagattacc tagttttaga gatataaaag aaaaatatca
     1321 tctaaaattt gaaaagaaaa aatacgtttt gcaagtattt catccagttg taaccgaact
     1381 agaaaatata gaaaaacaaa ttttagcttt gatcaattca atggaacaac aatctcaacc
     1441 aattatttgc attttaccta atagtgatgc tggtagcgaa attatcagaa aaatgtacca
     1501 gaaatattta aaaacggata ttatttatat tacgaattta gagccggagg aatacctgac
     1561 tatattaaaa gagtgcaaat ttatgatcgg aaattcttct tctgggataa ttgaagctgc
     1621 atcattcaaa ataccagtaa taaatttagg aaccagacaa aatggacgag aacaatctaa
     1681 aaatattata aatgcaaatc taaatgaaaa tgaaattgaa actgctttga ataaaataca
     1741 agatcctcag tttataaaaa gcttgaaaaa tgttaagaac atctacggtg acggcaacag
     1801 ttctagaaga ataacaaaaa cattgaatca aatattgaat gatggggaag atattaagtg
     1861 gattcaaaaa caaatagctt attaaaatat actagtgatc catacattat tattggggct
     1921 ggcggacacg caaaagtcat tattgaaacc ttaagattag aaaataagtt aatatatgga
     1981 ttttttgatg ataagcaaaa taaaatgcaa gatctgaaat atcttgggaa aataaacgaa
     2041 ttgaatagct gggcggatag caaaaaaaaa atctataaat ttattgtagc tattggtgac
     2101 tgtgtagtaa gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca atatggaata
     2161 gctgttcatc caagtgctat agtggcaaaa gaaatttcaa ttggttatgg aactgttatt
     2221 tttgcgaatg ctgtaattaa cccagatgct gtaattggag agcatgctat aataaacaca
     2281 ggtagcattg ttgagcatga ttgtagaatt aataactatg ttcatttatc tccaggtgta
     2341 tgcttagcgg gtggagtaca tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt
     2401 atccagatga agaaaattgg atcttggtgt atgataggcg cagggagcac tattgttaag
     2461 gatattccct cacattcctt agcttatggc aatccagcta aaattaagaa agaaggaatt
     2521 aattttgaat tctagaattt tattatcaag tcctcatatg ggaggaacag aagaaaaata
     2581 tgttgctaag gcttttaaag ataattggat agcgccatta ggtgaaaatg tggataaatt
     2641 tgaagaaagt attacaaaat atactggttc taagggcgga gcttctgcgt taagttcagg
     2701 tacagctgcc atacatcttg ccttaagact gcttaatgtt ggaaaaaacg atattgtctt
     2761 tgctccttca tttactttta tagctacagt aaatcctatt ttatatcaag gggctgtacc
     2821 agtgttcatt gactcagaac ctaatacatg gaatatgagc cctgttgcac tagagagcgc
     2881 attagaagaa gctgaaagaa acaaccaatt acctaaggca atcatcgtag tgaatttata
     2941 tggtcaaatg gctgattatg aacgaatttt agctattgca aaccaattta atgttccagt
     3001 gatagaagat gcagcggaat cgttaggttc tacttataag ggaatttcgt caggcttatt
     3061 tgggacaatt gggatctatt catttaacgg taataagatc atcacaacct caggtggagg
     3121 agctctagtg tctagtgata aaaaatatgt tgagcattca agatttttag caacacaagc
     3181 aagagatcaa gcaccgtatt atcaacatag cgaaataggt tataattacc gtctaagcaa
     3241 tatttctgcc ggtatcggac gtggacaaat ggaagtattg aatgatagag tagctaaacg
     3301 aagagaaatt ttcaatcgat ataaggaagc tttttctgga attgatagaa ttgaaatgat
     3361 gccagaagtg gatggcatgg tgccaaatcg ttggttgtct actttaacga tcaatgatta
     3421 taattctatt gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa acattgaaac
     3481 aagagctttg tggaaaccac tacatttgca accagtattt caaggggtaa aaatgtatta
     3541 ccatgataat aatcttagtg tgtcagaaaa gttatttaat agcggcttat gcttaccttc
     3601 tggaagtaat atgacagata gtgaacaaga atttgttatt gatgagttta aaaaaatatt
     3661 caagtaggtg tgatatttta tgaacatagg aattattggt ttaggttcca tggggaaaag
     3721 aagacttaga ctcattcaag aacactttaa tgatatttta ctatgtggtg ttgatactaa
     3781 tgaggcgaga cgaaaggaag ttgaaaagga atttggaata ccaacttata atagtctaac
     3841 agaggcaaaa aatttgtttg aaataaattc agtttttgtt tgtacatccc ctataaccca
     3901 tgaatttatt ataaaagagg ctttaaattt agattgtaat atctttacgg aaatcaattt
     3961 attaaatgaa tattatgatg aaataatcga agtagctaaa aagaaagata gattacttta
     4021 tctatcttct acatttttg
//
LOCUS       DASJON010000212         4032 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJON010000212 DASJON010000000
VERSION     DASJON010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.217"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            130..1074
                     /locus_tag="U3989_002571"
     CDS             130..1074
                     /locus_tag="U3989_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715988.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1370..2314
                     /locus_tag="U3989_002572"
     CDS             1370..2314
                     /locus_tag="U3989_002572"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1715989.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(2981..3379)
                     /locus_tag="U3989_002573"
     CDS             complement(2981..3379)
                     /locus_tag="U3989_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1715990.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(3376..3612)
                     /locus_tag="U3989_002574"
     CDS             complement(3376..3612)
                     /locus_tag="U3989_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1715991.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(3772..4032)
                     /locus_tag="U3989_002575"
     CDS             complement(3772..4032)
                     /locus_tag="U3989_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715992.1"
                     /translation="MYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTAGAKSATYF
                     FHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         1403 a          635 c          694 g         1300 t
ORIGIN      
        1 ccatagaaag tctttatgaa accaaaaata gctttgcata taatagcagt acagcgtgaa
       61 ctaataagcg aaagaaaccg cttacaaaaa ggagggagtc aattggaact agctgaatta
      121 gacatttttt tgcgtaacga agaatcaatc gaaactctgc agcgtgaaca aggaatgaat
      181 atcaatgatt taaacttagc ttaccatctt cagacaccgc caaaaatcca ccgaggggat
      241 ctatttcaac agggaaatat tcatatcagt aaacaccgtc gatatgcgga agttcccgag
      301 catacacatg attttgttga aatcaattat atgtatagcg gtacatgttt acaaattatt
      361 aatggagaaa ggatagagat ggaagaaagt gacgtggtga tcattgataa agaagctact
      421 caaagaatcg aatacacagg gcaagaggat attttaatca acatcttatt aaaagaagaa
      481 accttatcta cagagatatt gaatcatttg gcaagcacaa ataatctact ttcttctttt
      541 ttgataaatg catcaaaaga aaaaggggaa catacacaat tcattcattt tcgcggcgga
      601 aaaacaagtc gtttgacgca tttgattgaa aatatgctgt tgatttattt tgatacatac
      661 agtcataaga tccgttcgtt aaatctctat acatcactat tactgatcga attgactcat
      721 ttattagaac aacaaagctt ttcatcagca gatggatgcg cagaaaatga gatgcttaga
      781 gtattagatt atatcgataa gcattttact tcttgtacgt taaaagaaac agcagctcat
      841 tttggctaca atccggtcta tcttagtaat aaattgaaaa tgtattttga tgcttccttt
      901 caagaactgg ttctaaagaa aaaattcgac tttgcgttgg aactgattca agaaacgagt
      961 ttttcgttgg aagaaatcgc taatcgtatc ggatataaac aaacgacttc tttattcaaa
     1021 ttattcaaga agtattcaac atgtactccc aatgaatgga gaaataaaca ttaagaagaa
     1081 aatctttcac taaagcatat gaaggaaaat tgttttaggt gcgattgctg tcttattata
     1141 tcgttgcagc gaattttttt ggatttattt tcagttctaa acttttggtt gtattatcag
     1201 gggatatagg tgtaaacatt tgtcatttat ctgttatgcg tattcaggaa ttcgtttcca
     1261 tcaaaaatta taataacaac tattataatt aacatgttga taaaatgaat aaacaagact
     1321 atacttttca tgaaagtaac aaatagttat gaaaaggagc tgtttattca tgaaaaaaac
     1381 aagtcgtaaa gtagtaatcg tcggtacagg ttttgttgga acaagtatcg cttatgcaat
     1441 gatcaaccaa ggaatctcta atgaattagt attgatcgat gtgaatcaag aaaaagcaga
     1501 aggggaagca ttggaccttt tagatggtat ggcttggggc gatgaaaatg tggctgtatg
     1561 gtcaggtggc tatgaagaat gtaaagatgc tgatatcgtt gttatcacag ctggtatcaa
     1621 ccaaaaacct ggtcaatctc gtttagattt agttaaaaca aatgcgtcaa tcatgcgcca
     1681 aatcgtaaaa gaaatcatgg gatcaggatt cgacggtatt atcgttgttg cttcaaaccc
     1741 agtagatatt ttgacatata tcgcttggaa cgaatctggt ctgccaacat cacgtgttat
     1801 cggtactgga acaacattgg atacaactcg tttccgtaaa gaaatcgcat tgaaattaaa
     1861 agttgaccca cgcagcgtcc acggctatat cttaggtgaa catggtgatt cagaagttgc
     1921 tgcatggtca catacaactg tcggtggtaa accagtgttt gaaattgtag aaaaagacca
     1981 tcgtattgcc caagatgaat tagatgtgat tgctgataaa gtccgtaatg ctgcttatga
     2041 gatcattgat cgtaaaaaag caacttacta tggtattggt atgagtactg cacgtatcgt
     2101 aaaagctatc ttgaataacg aacaagctgt attgcctgta tctgcttatt taactggtga
     2161 atacgacgaa aaagatatct ttacaggtgt gccatcaatc gttgacgaaa atggtgttcg
     2221 tgaagttgtt gaattatcaa tcaacgaaga agaaaaagca atgttcagca aatcaacaag
     2281 tgctttaaga gaagtattga acactgtatt ataagaaaca atgaatatga actaaaaatc
     2341 ctaaaactgc ttaacagctg aaatagctgt taagcagttt tttattgata tctaataggt
     2401 aagcaaataa atagaatggt taaaattaat cggtccgaaa atgtatccgt acatatgaac
     2461 atatgaaatt taatctgttg attacaataa gcgaatcgtt atcaatgttt atctgtggtt
     2521 aattaaaaaa tccttccgca acggcaaata caatgattca tatctaattc gttcatattt
     2581 gattaactga ataaagaaac tggtttttat cattcaatta aacgacaaaa aaagtaagaa
     2641 aaactgcaaa atttttttat aattttgcat aagcagattt tgatggaaaa atgacgattt
     2701 ttgataaaaa accgttcgag agaggtttat cattttttaa atctactttg tataatatat
     2761 attatgtaaa ctaaaatata taaaaaatca gcccactagt tctaaaccac tatttcctac
     2821 tattcactct tttgtattcc ttttaaatca attatttcaa aattaatcca tattttccat
     2881 atttttatga aaaaactatt ttgtatgatt aaacataaaa aaatcagcat attccttaat
     2941 gacatgctga tctaattgag aaataaagtt tttaattcgt tcattttatt gcggtttgtc
     3001 gtaaataact ggttatcttg gactttagtg aatcgaaatc caatgaacta gtggtaatca
     3061 ttatgataaa atcaacagct tcttgacgag aaaaagcaac tggacaatta ttcaattgaa
     3121 gaaacaacaa catggcaaca agagcagttc gtttattgcc attatgaaaa ggatgttttt
     3181 tagctaaatt aattgctaaa atcgccgctt tttcatagat ttcaggatag agttcttgat
     3241 tgaatactgc ttgtgaaggc tgtttgacgg ccatatctaa tgcatttgca tcttttacac
     3301 cgatcatttc gcctggcgag tgacgtaaaa taactttcgc gttgattttg ataatttctt
     3361 tagtcgaaag atacctcata aatcaaccaa atcttttaac gcgtcttcat attgattcat
     3421 gatcgaatca actaatgaat caatatcagg ttcttcttgt gctttgatca ttcgtacaga
     3481 accatcagat tgaaaaacat aactgattgc gtcacctgtc tgcacaccca tgaattcttt
     3541 tacttctggt gggatagttg ttacatcgct attgccagat tttctaattt tatacttttt
     3601 catttttttc atatttcgcc accctctata ccattctcat tctatagtaa gtacattata
     3661 tccaagtata tacttttaat caatgtatat acttttattt tgctttttct tacgaaaaaa
     3721 tgacaaagaa aaaagtttgt gacatgactt ttgtcacaaa ctctcgtatt cttaaccaaa
     3781 atattgatga aatacttgtg taataactgg tagaaaataa ataatgatca tagccgctac
     3841 caccaccaac aattgttttc gctgtttctt tcgctttagc atgcttgtcg ttttatgaaa
     3901 aaaataagta gcgcttttag ctcctgcagt caaagtcgat aaaatcaacg ttggtgaata
     3961 tgctgtttga ttgtgcgtca atgacaacaa cttcgtcccg taatcaggat actttagaaa
     4021 tgcttggtac ac
//
LOCUS       DASJON010000213         4031 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJON010000213 DASJON010000000
VERSION     DASJON010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.218"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..163)
                     /locus_tag="U3989_002576"
     CDS             complement(<1..163)
                     /locus_tag="U3989_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1715993.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNE"
     gene            complement(230..1600)
                     /locus_tag="U3989_002577"
     CDS             complement(230..1600)
                     /locus_tag="U3989_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN1715994.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(1678..2214)
                     /locus_tag="U3989_002578"
     CDS             complement(1678..2214)
                     /locus_tag="U3989_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1715995.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            2523..4004
                     /locus_tag="U3989_002579"
     CDS             2523..4004
                     /locus_tag="U3989_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1715996.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         1300 a          809 c          592 g         1330 t
ORIGIN      
        1 tttcatttcc atcaaatttg tcatagacga atttttttac tttcgtccga gtagtcatat
       61 gatggatctt ttccattact tttcccgata caacgactgc tgtcgctaca ctggcagcgg
      121 ctgctacgct taatccgatt gttacttttg ttgatgtttt cataagtatc agctcctttt
      181 tatctatctc catcatagca tagacaaaaa aataaacgaa ctaatccact cagtgaaatc
      241 tcaatccttt agggaaaaag ttctttactg ttccttttac ttgtttccca aatccgacag
      301 gtatcttatc taatactagc aaaacccatc cttgattccc atcacgttga aatgtttctc
      361 ctgctgtgta agattgccat tccttttgag tgatcggcaa gcaaggtata ttctctatct
      421 tttttgtagc aagcgctaat gcatagctag gttcaaatcg attctttttg aaatctccta
      481 aatgcaaacc tgtacgtacg acttttaaac catcgagaga aggggctaac tcaggaactt
      541 cccaaagatg gtcgttgaac actaaaagtc ttccagtagc ttcataatga aaatcatttg
      601 aaaactccgt ccagagtttt tcctgctctt ttgtcatttg cactttactt ttttttcgtg
      661 tcttcttttc tttatgcatc tgattttgtc catgaaaagt cagctttgca acaaaatgtc
      721 cttcaccctg atctttatga ggccatatac gtattgtttt ttccaaacca gctacgcttc
      781 cccattcaga acgtcctgaa ctaacggatt gagtcaaagg aatctcctca atcgttactg
      841 gataattttc tacaagccag ctaatgatct cttcattttc ttctggtgca aacgtacaag
      901 tcgaataaat cagttgtcct ttatttttta gcatttttat tgctgaagac aaaatctctt
      961 gctgacgttt ttgacagtat agcggagatt cttctgtcca ctcttttatt gcatttggat
     1021 cttttcggaa cattccttct cctgaacaag gagcatcaac cacgatccga tcaaaaaagc
     1081 ccgaaaaatg agggacaagt tctgcgggtg catgatttgt cactattgcg ttcgaaactc
     1141 cccatcgttc tatattttct gataaaatct tcgcacgttt cggaaagatc tcattcgtca
     1201 ctaataaccc tttccccttc atttgagcag ctaattgggt tgatttacca ccgggagccg
     1261 cacacaaatc caaaactttt tcaccgggtt ttgctgcagc agctgttcca acgatcattg
     1321 cacttggttc ttggctatat tcatacccag cttgatggag gaaagacttt ccattgacag
     1381 tgccaagaaa tccctcattg ctgtagggag cgggttgtaa ttcttcgcta tggtaagttt
     1441 ggaccatgtc caatcctgcc ggttttagtg gattccaccg aaatcctttt ttcacgcttc
     1501 cttgttcaag cgcagaaaaa aaatcggatg cttcttcgcc taaaagaaga cgatattttt
     1561 tgataaattg ctgtggtaac gttgtcgctt cctccttcat tccaatgttt cgttagacat
     1621 tttacaatct tttttacgaa aaataaaggt ggctgatcta ctttttcatg ttgctgccta
     1681 aggtgactgt tcctttaaga tattttccaa gtattcatcc gtcacctcgt ttttatccca
     1741 aacagcgttc atcatttcaa gaaaatcttg agaagaatca tctgagcgca aacttcccaa
     1801 tgcattaagc cataccaatt caagcgactt ttcttctttg atcttcgtgt gacctttgac
     1861 atatgagaaa aatggccgct taaatagcga atccatgtct aaatcagatt ttgcaatgat
     1921 ttcacgaata ctttcatgga tagaaaaaaa atctttcaat aagtagacaa tgcttaaaac
     1981 atcatcggaa ctgcattcag ctaagtagtt ttcaaaataa acagtgattt cttctaatat
     2041 cttctctttt aacccgctga ttgatttgaa atgtgtataa atcgggtatg tagaacaacc
     2101 gcagaactct gctaactctc ttgcagtaat tgacttcatt ccatattctt ccacgtatac
     2161 catacttttg aacagcaaat attctttact ataatttgtt actctcccca tcattcttac
     2221 ctccgtctct tttcatctac ccataaataa ataacataaa taattctaaa aatattattg
     2281 ataattatca aaacatattt agttatatcc attaaaaatc aatttttccg ccttaaaaac
     2341 aagaatatct tgacaaaata ccctaggaaa taaacaatag aataaacaag gtaacaaaaa
     2401 aattaaataa cgttatataa aaaagaaggc gacactctgt gagaataaca cagactgaaa
     2461 tgactttcca ctcacagttt ttgtctaact atatacaaaa acagaataaa aaggaggtat
     2521 ttatgctaat tgaagcaaga aaaaaagagt tacttgttct acaaatggtc attcaaaaaa
     2581 ataattgttc tttacaagaa ttatccaatg acttacatat tccaaaacgg acaatcaaag
     2641 aactgatccg aaaaataaat gtatcactca agcaattttt attgattgat gaatttattt
     2701 attctaatca taaaggagag atccaaacca aagaaaccga tcaaactaaa aagttagctg
     2761 ttttttctaa gttaaaatta tgctacctaa aagaatccaa tcgttttaac tatttgatgt
     2821 tactaatcaa ttatccacaa actgctgtac caaaaaaata tttacttgaa caactttata
     2881 tatcgccctc ctatctcgaa aaactgacaa aacagctcaa tcattcctta aaaaaattcc
     2941 atttacggat cctatctgta aaaaattgct gtactcttga aggaaatgag ttatctatcc
     3001 gtctctatct ttactttttt ctttcagata catttggagg taacgattgg ccattttcct
     3061 cacttgaatt aaatgcacta aaaatgaaca gtacagatga gcaaatagaa aaaatttcaa
     3121 aacaagaaaa aaatatctac ttgctgttaa ctttattcat attacgaaac aaacaacagg
     3181 catattttcc aaaaacttct tctgaaatca ctgaattgat ggacttattg cagcatacac
     3241 atgattattc agaatacttt acttcttttt cagtgagtcg cccttgtgaa gagacagaaa
     3301 cagagagagc ctactttaat tattttttct attgtttttt tccaaatcaa atctcttttc
     3361 gacaaaaaca ggagattggt tttttatttt ccacaagcaa aaaccttatt tgtcgacaag
     3421 tcggtcaatt gatgacttac ttaactcaac agtttcctct caaccattta gaaaataaaa
     3481 taccaattta tttttatttt cttgtgttgg cagttgtttt tttcgaactg ttgggtgaat
     3541 ccagcagtag tttttctaca ctttgctttc cagaagccac gtccaatcat tctagttctt
     3601 ttatacagga aaagaaatta agagaaaaaa tcgatacaat tttttcaaaa aatggggaaa
     3661 gaaaccaaat cagtcaatac cttagtcaat tgatccaaca cttactacaa acagaaacga
     3721 aagagcagtt actcatccat ttgcagataa ccaaacagtt tacaggaaat tatcatcttc
     3781 aaagccattt gcagcagtta tttcgcaaag atgctgttaa aattaccgca tattcagaac
     3841 atgctgattt gatcattaca gatcagcatg agaaaatttc agatgaaacg gagactttct
     3901 atctggatac aacgcacaat acagtgttat ttggcaagtt aataaaagcg gttcatgaac
     3961 tgtatatgaa aaaaggttat gagaacaaaa caaagaggat gtgacaaaat tcttgtcaca
     4021 tcctcttatt c
//
LOCUS       DASJON010000214         4013 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJON010000214 DASJON010000000
VERSION     DASJON010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.219"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(644..1624)
                     /locus_tag="U3989_002580"
     CDS             complement(644..1624)
                     /locus_tag="U3989_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN1715997.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1637..2515)
                     /locus_tag="U3989_002581"
     CDS             complement(1637..2515)
                     /locus_tag="U3989_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN1715998.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2531..3331)
                     /locus_tag="U3989_002582"
     CDS             complement(2531..3331)
                     /locus_tag="U3989_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HEN1715999.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(3606..3755)
                     /locus_tag="U3989_002583"
     CDS             complement(3606..3755)
                     /locus_tag="U3989_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716000.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
BASE COUNT         1161 a          817 c          628 g         1407 t
ORIGIN      
        1 taaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt
       61 cttcgccaac acaatttgac gaagagcctt ttctttcagg aagacttcgg taatttttag
      121 gagttctacc tattctttta accgtttatt ctctttttct aagcgtgtaa tttcatttct
      181 gcgaatggat ttcatgactt cctctaccga gaaaagtacg ttatccatat ttttcaactt
      241 cttgaactca tcaatacacg ctattagagc gagcttctag ctatagacta acttctttaa
      301 cagaaaaccc ttcttccgtt accagtttta cagcggtaat tttaaatatt tcatcaaatg
      361 aacgtcttgg cataatccag atcttcgctt tctcttaatt tggtgtcaat tttagtataa
      421 cccgtccaac ttatttttta actaagtgcc cggtttttct tgacaactcc acccaaaagt
      481 tagatttttt ggtctaagtt ttagggtcgc ttcattacta gaattcaaca agagatgttt
      541 tttattttat ttaaattatg gggtcagttt ccatttgatt acaaacttct ttgttttctt
      601 tttttaaacg caataataaa ggttttaatt tcagcctttt taattagaaa atcaattttc
      661 ccaaattatt ttctttagct ttcttaacga tttctgtcgc aactaccaca tctaatacag
      721 ccgaaccaac tgttttgaag atcgtgattt gttctccact tgtccgaccg atcaattctt
      781 cattgatcag ttggcctagt tccccatgat agctgtctct ttgaatatac ccatcttgca
      841 gcggtgaaat gaagtcccca gcttctttta gaacaccgtc catggtatca aaaataacaa
      901 catctgcggc tttgattatt tctcgtggaa tttcacacat ttccggtgtg taagcaccga
      961 ctccattgat gtgcgcacct tttttgaccc actcagcaga gaatgtcgca cgttttgaag
     1021 tggtcacact tgtaatgatg tctgctcctt caacgcattc ttgactggtt tttgttgggc
     1081 gcatcgttac agaaaattgt tgcatcattt tttcagcaaa ttggctggca cgctcaaaat
     1141 caatatcaaa aatccgaact tcttctaatt tacgaacggt caacattgct tctaattgtg
     1201 actgtgcttg tccgccggtt ccaatcagcg cgccaatttt agcatcttct cttgcaagaa
     1261 gttctgttgc tgctccttga acagcgcctg ttcttaattg tgtcaaataa gtaccatcca
     1321 aacaagcgga taccataccc gtttctggat ccaatacgat catcgttgct ggtacacttg
     1381 gtaagttttt cttgatgttt tcaggataca cggaaacgat tttgacacct agcgcgcctt
     1441 cgccaccttc tacataagca ggcatatata agctttgtcc gttgcttttt ggcacatcga
     1501 tattggtgcg aagaggcaca cttgcctttc ctgctgaata caatgacagt gcttttttgt
     1561 ccgcatcgat ggcttctctc attgaaaaac ttttgataat atcttctttt ttaagaaata
     1621 acatttgaat cgctccttaa ttagctgctg taactttttt taattgactg cccaacatcg
     1681 tatatgcact gtatagtaag accaaaatca cgatccattt caggatatag gtatctaaag
     1741 atttgactag gaatacagcg accaataccc ccaatacacc gaaggttgaa gtaaaaagtg
     1801 tgatttttct gctgtattca ttaaatttaa tgaattgaac actgccgatc ggtactgaga
     1861 aggtacaagc ccccatcata ataggaaagg ctgcaactgg attcaagcct agcaagtaga
     1921 cagttaccat cgttaacgca taagaaccaa tgccgatatt gttcaatgca ccatagacaa
     1981 agagtaaaaa tcctgccaag attaattttc cgccatacaa ttcactggct gttccgtttg
     2041 atggaatcca gttcatttgt cctgcaaaaa tcaacaatgc agcaatgacc aatcctatgc
     2101 cgacaaacag tttgatcgtt ttctctggta acttcacgac aaatcttggt cctaaatatg
     2161 caccgataat ttggcagata atacaaacag ctagtgtctt gatccctacg ttgatggcag
     2221 tgatgtaaga caatgccatg acggctaccg gtactacaca ttgggtattc aacgttccgg
     2281 gtagtttttt catgctgacc catttcaact ttggataaag gacggttccg atagcaaagt
     2341 cggaaacgcc aaatgttgaa agaaagaaga tgataaatga cgtgaagggc aacagacgca
     2401 tatcccctgg ttcttctttg aaattttgtt tgtttttgaa aaggtctctt agaaagtaaa
     2461 taataaaata tagattgatt gcgacaatta gtgtcagcaa aatattccga atcataaaaa
     2521 attcctccta ctagtaaatc tttctgattc cctcaaagcc ctcaccatat tttgctttaa
     2581 gttcttcagg agattctgca atcactttta gtgtatatgc atcatgtttg acatattctt
     2641 ttgctagatt gaccatggcg tggttttttg tccaagtgac atcggttgat tgcagttctt
     2701 cgttaatggt accaaacacg acttcttgct catcggcgac gatgttgatc catcttgctc
     2761 caaagtcagc aagtttgtct gtttcaaaac catgaatata atacttttct aagggaagat
     2821 catattcatg ggtagaactg aataaaatca gtacaaactt atcaactctt ttttcagctt
     2881 gctgcaatgc ctttagtagt gaatccgtga aattctcatg ccaaacttga atgagcaaac
     2941 tttcttttgc gtttttcacc aaatgctcta ctttatccag aacatactcg ataccatcaa
     3001 ctttccacag catttcttct tcatcttttt ctttgatcat ccctagattc ttggtaagaa
     3061 aagaaagatc atttttaaca cttttttcta atttctctaa aaactcattt gccgagatgg
     3121 catgataaag tttaggttct gatttattaa cgacaatcaa attctttttt aatagttttt
     3181 ccaaaatatt atatactttc gagcgtggca cacctgagat tttacttact tcatatcctg
     3241 taagattacc tttttctaat agtgtggtat acactcttgt ctccatctct gaaaaatcat
     3301 attttttcat gatttgaata atcgtttcca ttgattaatc ctcactttat ttttagtagc
     3361 tactacggta acaactataa attctttttg attatctgtc aagagttagt tcaataacac
     3421 acatataata attagcttaa aaacaatctt tttattaaaa agaaacatta taatatgaat
     3481 gtttgtgaaa aaataataca acttagtcat tctttacaac gatttataga aaaacaacct
     3541 ctttaaatat aaaaggtacg ggttactgat tacttgtaaa tctttacgtt ccagttttat
     3601 tctttctaaa aaatatactc acttcttttc ttagacttct ttttttgatt ctcctcttta
     3661 tggtcttgtc cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa agcatttcta
     3721 ggttttggac aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga
     3781 tatcatcaca ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag
     3841 atttcactgt gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta
     3901 atctaagcga tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg
     3961 gaaaaacttt tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttg
//
LOCUS       DASJON010000215         3925 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJON010000215 DASJON010000000
VERSION     DASJON010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.220"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(573..1130)
                     /locus_tag="U3989_002584"
     CDS             complement(573..1130)
                     /locus_tag="U3989_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716001.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="U3989_002585"
     CDS             complement(1309..1920)
                     /locus_tag="U3989_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716002.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="U3989_002586"
     CDS             complement(2033..2290)
                     /locus_tag="U3989_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716003.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="U3989_002587"
     CDS             complement(2887..3165)
                     /locus_tag="U3989_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716004.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="U3989_002588"
     CDS             complement(3199..3450)
                     /locus_tag="U3989_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716005.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DASJON010000216         3873 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJON010000216 DASJON010000000
VERSION     DASJON010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.221"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1107
                     /gene="uxaC"
                     /locus_tag="U3989_002589"
     CDS             <1..1107
                     /gene="uxaC"
                     /locus_tag="U3989_002589"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1716006.1"
                     /translation="PLYSWSHLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRR
                     EIAKKSNVEVICTTDDPADDLHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVT
                     LLEEAADQTITTYQELIEVLGQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQK
                     ARANKLLSSDEIDAYRTETLIHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGY
                     DGMNDLSLTIPLQKLLNRAEETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQL
                     GSGWWYNDTRAGMRDQLTQFADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGE
                     MVMRGEAPEDEQLLGSLVQAISYTNAKNYFGFFA"
     gene            1266..1949
                     /locus_tag="U3989_002590"
     CDS             1266..1949
                     /locus_tag="U3989_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN1716007.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            1951..2787
                     /locus_tag="U3989_002591"
     CDS             1951..2787
                     /locus_tag="U3989_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1716008.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            2798..>3873
                     /locus_tag="U3989_002592"
     CDS             2798..>3873
                     /locus_tag="U3989_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1716009.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYP"
BASE COUNT         1229 a          760 c          746 g         1138 t
ORIGIN      
        1 ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga cttgttgata
       61 aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc agctcctgat
      121 tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac aacagatgat
      181 cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga tttgaaggtt
      241 ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt taatgcatgg
      301 gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga actgatagaa
      361 gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc cgaccacgct
      421 ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat ttttcaaaaa
      481 gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcgtac agaaacactg
      541 atacatttga cccacttcta tcattctcat aattggacga tgcagctgca catccatgct
      601 tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg ctatgatgga
      661 atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc agaagaaacg
      721 gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc tgcgatttta
      781 gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg ttctggatgg
      841 tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc tgatggtagt
      901 gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc ctacactaga
      961 catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat gcgaggagaa
     1021 gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta tacaaatgct
     1081 aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga atcaaaaaca
     1141 ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg gaccaaattt
     1201 gctccgtctt cttctcttgc tgttctgctt cattgcctat aaacctttat gaaagaagag
     1261 ataacatgcc tactatccat attgccggtg attctactgc ggcagaaaaa gatgaaaaca
     1321 agcgtcccga gacaggttgg ggagaaaaaa tctctgctta tttttcaccc gagatccaga
     1381 tcaataacca agcaaagaat ggacgcagca ccaaatcatt tatccaggaa ggaaggttag
     1441 cccgtctagc ttcacagttt cagccgaatg atttcttatt tgtacaattt ggacataacg
     1501 accagaaaat ccaagaagaa agaggtacgc ttcccttcgg tagctattta aaaaatctgc
     1561 agatattttt tgaatgcgca aaaaaagcga acgtccaaat ggtcttactg acatctgtga
     1621 cgcgacgaga ttacttagaa aacggaacat tgaatccaga tatacttgga gactatccaa
     1681 aagcgatgcg tgcatttgct gagaaacacc atattcctct tttagatgtg ttctcaagat
     1741 cacaagaatt gttccagacc ttctcaaaag aagagacaaa aaaattctac ctgcatctta
     1801 tacctgatac ttgtaaaaat tacccagaag gactaaaaga caatacccac tttagtccag
     1861 aaggcgctga taaagttgcc cgaataattg ttgaattgat aaaagaatcc cgtctgcctc
     1921 ttgctaatta tttacaaaaa ggagtttgag atgtttccat taaatttagg catccgtgcc
     1981 cacgatttag atgttcgaaa tcgtgaagaa atcgttcaaa aagtatcaca gcttcacttg
     2041 tcccatatcc aatttgcacc acaaaaagca tttcctgaac aacttaaatt aactgacatg
     2101 acacttggga gtgctgccta ttttggtgaa tacttccaaa aaaacaatat cgaactttcc
     2161 attttaggat gctatatcaa cctatccagc aaaaatcaag aagttcgtgc acaagcattg
     2221 gcaactttca aacagcaaat tgctctgtgc tctgcctatc aagccaagat gattgctacg
     2281 gaaaccggta gtgtctctgt cggttacaca aaagaaaatt ttactgaaga agcttatcag
     2341 atcgccagaa attctatcat tgaattagtc gctcatgcag aaaattttgg cgtaactgtt
     2401 gctgttgaag cagggatcaa tcatccaatc tataactatc agttagctaa acgtttaatc
     2461 gatgaagtag cttcaccaaa tttaaaaatc attttagatt gtgcgaattt gatgcataaa
     2521 gaaaatcatg ctgatcagga aaaagtgatc cgaggtgcct tggacaattt acatggttat
     2581 attacagcgc ttcatctcaa agactatatc atggaaaatg gaaagatccg catggtacct
     2641 gttggcaagg gctgtttaga tttccgcccg gtactgcatt atatcaaata tgagcgtcct
     2701 ctaatgtacg ccactcttga agctactcct gagatttatg ttcctgaagc aatagaacac
     2761 ttgcagttta tttaccaaga ttgttaagga gaagattatg gaggaacgaa tacagaaaag
     2821 ttatcaaaaa tatcatgcaa atgcttttca gacattgctg catctttatc agggaaatta
     2881 ttggcgtttt gccatgtcag gactcttttt tattatcaaa catcttccat cttggttaat
     2941 gccgatcgct attgccaatg taatcaatgc agcttcatcg aaagattctt ctcagttgca
     3001 ggccatttat ttcaacgctt ttttgatggc ttgtctgatt ggtcaaaata tattgacgaa
     3061 ttattttcat gtaaaatacc atagcacttc gatccgtcaa gtagaagctg gactaagagc
     3121 gacccttatc aagaaaatcc aagaactatc tattccttat caaaaagaat tgcaatctgg
     3181 acgaatccag tcaaagatca tccgtgatgt agaagctatc caaacgttgt cctctcaagt
     3241 atttgtcagc ggattaaata tcgctgtcaa tttattcgta gctttaggta tcacgcttta
     3301 taaaagtcct gttgttttca tgtttttctt attgactgtt cctctcgccg cattcgttac
     3361 tctctttttc cgtaaaagga tcagacagtc caactcagac ttcaggcaag aaatggaaga
     3421 gacttctgca aaagtgatcg aagtgatcga attagcaccg attgctcgag ctcatgggtt
     3481 agaaaaacac gaaacaactc gcttaaataa gcattttact caaatctatg aaaaaggatt
     3541 ccacttagat atcctgcaat ctgttttcgg atcagtcagc tgggctagtt ttcagatatt
     3601 tcagctgatt tgtttgattt tcactgggat attagcttta caagggcgca tccaacccgg
     3661 cgatgtcgtg atgtatcaga cgtatttcac gacgatcgtc aattccgttt ctggaatgat
     3721 cacgctgatt cctacgattt caaaaggcat ggaatctatc acttctattg gcgaactgtt
     3781 gattgctggc gatgtagaaa cttatcacgg taagaaaaaa atccaacatt tggatggaaa
     3841 ttttcgtttt gaggatgttt cttttcttta tcc
//
LOCUS       DASJON010000217         3872 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJON010000217 DASJON010000000
VERSION     DASJON010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.222"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..536)
                     /locus_tag="U3989_002593"
     CDS             complement(<1..536)
                     /locus_tag="U3989_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN1716010.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQL"
     gene            728..1081
                     /locus_tag="U3989_002594"
     CDS             728..1081
                     /locus_tag="U3989_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1716011.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(1155..1811)
                     /locus_tag="U3989_002595"
     CDS             complement(1155..1811)
                     /locus_tag="U3989_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1716012.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            2037..>3872
                     /locus_tag="U3989_002596"
     CDS             2037..>3872
                     /locus_tag="U3989_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1716013.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLN"
BASE COUNT         1263 a          758 c          729 g         1122 t
ORIGIN      
        1 agctgatttt ttggttcatt cttctcactt tgttcttctt catggacatg atgatggtga
       61 ttttctgcta ctttagacgt gattttttct ttcaatacag cgtctttttg aaaataggca
      121 agccagacac caatcacaag ggcaacaaaa aagctgccta tcacccgcca gcttgccatc
      181 tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca cgattggatt gatgatcgga
      241 gcagtcagca taaaagcaaa cgctgtgtga actggtacgc cttttttaac aaattgatga
      301 acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta
      361 aaaatagaga agatacgatt ttcaggaagc catctcttca cacgttctgg ggtcaaaaat
      421 acttgtaagg cgcctgagat aagacagcct aataatacga aaggtaatgc ttcaataacg
      481 atcgataaga aaatcgtact catctgcaaa accgaatttg gaataaaaga aaacatgagt
      541 ttcctccttt ttatttctag ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa
      601 caagttgctc tgtaaatttt tacacaactc ttatttttta ccaaagcact tcaattgact
      661 ttttctgtta ttcccattaa attaaggtct catacatatc acttctatca taaaagggag
      721 aataaaaatg aaacaaacca ctaacttgac tgaagttcaa gacatactgc aacagtcatc
      781 cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg
      841 actagaggaa cttctcacac attatgatat tccggcactt catttggatg catttgaaac
      901 accagaagta gctagtaaat ttgaagtgct gactgcgcca gttgtcctga tttttcatca
      961 aggaaaagaa gtctttcggc aagcacggtt catcgacttt aaaaaaatcc gttatctact
     1021 agatgaactg acagcagaag atgattccct ttcttatgaa gaaatcttcc gaacaaaata
     1081 atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa gttttgcctt catttgttca
     1141 atcgcttttt atttttattc tgctgtattt tcggaatgcg catttgcgtc agaatgcaag
     1201 aaatagatca aggtttgcaa ttcattcgtc aaatcaacat tttggatcaa tacatctgac
     1261 ggagcattca atcgagcagc ggtaaagttc aagattcctt tgattcctgc ttccgttaac
     1321 tgattgacta cttcttgtgc tctttcagcg ggtatcgtta aaattgctac ttcgatttgt
     1381 tggatcttga tttgttctga catatcttcc attggataaa cggggatacc gtctacgatg
     1441 cgcccaacga tttcctcatt cacatcaaaa gcacaactga tacgtatgct attactttga
     1501 tggaatttgt attttagtaa agcacttccc aagttcccta caccgatcag cgcaacattt
     1561 gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca taagatcttc tacatcataa
     1621 ccatacccac gttttcctaa ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta
     1681 tctacttgaa cggcttcact taattcagtt gaagacactt ttttctttcc agcattattt
     1741 aaaatcctta aatagcgata atacaaaggc agtcttttag ctgtcgcctt tggaataata
     1801 tgatctttca cgaaatgacc tccacgtatt gtctaattac atctttgatt tctccatcat
     1861 agcagtattt ataggaaaaa atcaattcca ttgctcagat attgtgaaat tttattcttt
     1921 attcatttat ttatcatttc tgcaaatctc gcagtataat ggaatttatg gtagactact
     1981 aattgaaaaa tagtttttta tttgaggaga attcacaaag tagaaaagag gattccatga
     2041 tattattaca agccaatcag attgctcgcc attttggtgc agaagtctta tttgaaaaca
     2101 tacatttaga aatcagtaca ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa
     2161 aatcgacttt attaaagatc attgctggaa ttgaagcacc tgatgaagga aagatcgcta
     2221 aaaataaaac agcgactctc ggatacctcg ctcaggacac aggacttgat tccaatgaaa
     2281 cagtttggaa cgagatgctg aaagcttttg aacaagttag acaaatggaa gagcggatgc
     2341 gcgagctaga gattttcatt agcgaaggta cgccagacac acctgcttat agttcttatt
     2401 taaaagaata cgaccggctg caacacgatt tttcagatcg aaacggctat ggttatgaaa
     2461 atgaaattcg ttccgtattg catgggttta aattcgattc ttcattttat gatcagaaaa
     2521 tcgatacttt atcaggcgga cagcgcacac gtttagcttt agctcgcatg cttttgcaac
     2581 accctgacat tttgattcta gacgaaccaa cgaaccattt agatatcgag acactttcct
     2641 ggcttgagaa ttatctgcaa agctatactg gtgcattatt gattgtttcc catgaccgtt
     2701 attttttaga taaagtagtc aatgaagtct atgaattaag cagaagaaaa atgcgccatt
     2761 acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga acagttagca agtgaatgga
     2821 aggcttatga aaagcagcaa acagaaatcc ataaattaga agacttcgtt gctcggaacc
     2881 ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg
     2941 accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa ctttctattc agtatagaaa
     3001 agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc tatcggatat gataatgtca
     3061 ccttatctga accaatcaat ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc
     3121 cgaatggtat cgggaaatcg acgttgctga aatcaatcat tcaccagatc ccttttatcc
     3181 gtggaaaaga aacgctaggg acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg
     3241 atctgcatac aaacaagacg atcctagcag aattgtggga tgagcatcct accactccag
     3301 aaaaagagat ccgaagtgct ttaggtgctt tcttattttc tggcgaagat gtagaaaaac
     3361 caatccctct gctaagtggc ggtgaaaaag cacgggttgc tttagccaag ttagcgatga
     3421 acaaagaaaa ttttttgatt ttggatgaac caacaaacca tttagatatt gacaacaaag
     3481 aagtattaga aaatgcattg atcgattatg acggcacact gctttttgtt tcccatgacc
     3541 gatatttcat caatcggatc gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag
     3601 tctatctagg agactatgat tattatttag agaaaaagca ggaagaagaa gaaatcgccg
     3661 aattgttggc acaagatgag ccagaatctg ctcctgctcc cacgaacaaa tctgattatt
     3721 accaaagcaa agaacagcaa aagcttatcc gtagccttca acgaaaaatt actcaggttg
     3781 aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc
     3841 cagaaaacct ggaagatcac gtaaaactca at
//
LOCUS       DASJON010000218         3863 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJON010000218 DASJON010000000
VERSION     DASJON010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.223"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(245..466)
                     /locus_tag="U3989_002597"
     CDS             complement(245..466)
                     /locus_tag="U3989_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716014.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="U3989_002598"
     CDS             complement(564..809)
                     /locus_tag="U3989_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716015.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="U3989_002599"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="U3989_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="U3989_002600"
     CDS             complement(2033..3034)
                     /locus_tag="U3989_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN1716016.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="U3989_002601"
     CDS             complement(3193..3459)
                     /locus_tag="U3989_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1716017.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="U3989_002602"
     CDS             complement(3449..3805)
                     /locus_tag="U3989_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1716018.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DASJON010000219         3851 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJON010000219 DASJON010000000
VERSION     DASJON010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.224"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(3..1157)
                     /locus_tag="U3989_002603"
     CDS             complement(3..1157)
                     /locus_tag="U3989_002603"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN1716019.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1342..3783
                     /locus_tag="U3989_002604"
     CDS             1342..3783
                     /locus_tag="U3989_002604"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN1716020.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1232 a          721 c          840 g         1058 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     1261 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     1321 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     1381 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     1441 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     1501 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     1561 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     1621 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     1681 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     1741 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     1801 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     1861 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     1921 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     1981 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     2041 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     2101 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     2161 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     2221 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     2281 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     2341 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     2401 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     2461 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     2521 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     2581 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     2641 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     2701 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     2761 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     2821 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     2881 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     2941 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     3001 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     3061 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     3121 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     3181 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     3241 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     3301 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     3361 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     3421 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     3481 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     3541 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     3601 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     3661 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     3721 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     3781 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     3841 atcttgcttt t
//
LOCUS       DASJON010000220         3792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJON010000220 DASJON010000000
VERSION     DASJON010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.225"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            14..1141
                     /locus_tag="U3989_002605"
     CDS             14..1141
                     /locus_tag="U3989_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1716021.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="U3989_002606"
     CDS             complement(1359..2501)
                     /locus_tag="U3989_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1716022.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="U3989_002607"
     CDS             complement(2506..2691)
                     /locus_tag="U3989_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1716023.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="U3989_002608"
     CDS             complement(3045..3290)
                     /locus_tag="U3989_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1716024.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="U3989_002609"
     CDS             complement(3284..3685)
                     /locus_tag="U3989_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1716025.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1248 a          685 c          576 g         1283 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa ac
//
LOCUS       DASJON010000221         3763 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.226, whole genome shotgun sequence.
ACCESSION   DASJON010000221 DASJON010000000
VERSION     DASJON010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.226"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..2364
                     /locus_tag="U3989_002610"
     CDS             37..2364
                     /locus_tag="U3989_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1716026.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2490..2819
                     /locus_tag="U3989_002611"
     CDS             2490..2819
                     /locus_tag="U3989_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1716027.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2859..>3763
                     /locus_tag="U3989_002612"
     CDS             2859..>3763
                     /locus_tag="U3989_002612"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1716028.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLG"
BASE COUNT         1325 a          715 c          742 g          981 t
ORIGIN      
        1 aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa
       61 aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa
      121 agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc
      181 ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca
      241 gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct
      301 agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa
      361 gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag
      421 acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat
      481 acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat
      541 aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc
      601 cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc
      661 tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa
      721 agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact
      781 ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat
      841 gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg
      901 gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca
      961 aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc
     1021 actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta
     1081 gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat
     1141 caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt
     1201 gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt
     1261 tcagcgatag aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat
     1321 ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat
     1381 aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct
     1441 gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac
     1501 aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa
     1561 aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca
     1621 aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca
     1681 tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca
     1741 tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt
     1801 tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca
     1861 gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac
     1921 aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact
     1981 tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat
     2041 gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa
     2101 tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg
     2161 attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct
     2221 aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc
     2281 tttgtctttt taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag
     2341 aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg
     2401 ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat
     2461 cacctattac atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca
     2521 gtcaaaacga cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg
     2581 ttactttcgc tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta
     2641 gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag
     2701 aaaatgtgac aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca
     2761 aagatgagca aggtcgtgac gctgtttacc gatttacgga aataaagact tcagattaag
     2821 taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata
     2881 aattgacgac catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat
     2941 taacaaaaac agtagagaat aagcagacag attttgcaga tggggataaa gtcccttatc
     3001 tcatcaccgt aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata
     3061 cagctgatcc tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca
     3121 agattcatac atttcacaca caaaaaataa aacatggttt tgtcttatca gattctggaa
     3181 aagatcttag ttcgtttgct ggaaaaaaat taacgatccg ttaccaaatg acattgaaag
     3241 aaaacagtga aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag
     3301 tagaaacaca tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa
     3361 cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc
     3421 aagaaaacgg gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt
     3481 ccagtaaaga tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg
     3541 agattaaacc gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa
     3601 aaggcagtta tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa
     3661 atccgacgaa taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa
     3721 aaaatggagg aagctttcca aaaacaagcg aagagatgct tgg
//
LOCUS       DASJON010000222         3730 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJON010000222 DASJON010000000
VERSION     DASJON010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.227"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            363..731
                     /locus_tag="U3989_002613"
     CDS             363..731
                     /locus_tag="U3989_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN1716029.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="U3989_002614"
     CDS             911..1852
                     /locus_tag="U3989_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN1716030.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="U3989_002615"
     CDS             1921..2652
                     /locus_tag="U3989_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN1716031.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..>3730
                     /locus_tag="U3989_002616"
     CDS             2796..>3730
                     /locus_tag="U3989_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN1716032.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLR"
BASE COUNT         1249 a          627 c          825 g         1029 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa
//
LOCUS       DASJON010000223         3695 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJON010000223 DASJON010000000
VERSION     DASJON010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.228"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..951)
                     /locus_tag="U3989_002617"
     CDS             complement(<1..951)
                     /locus_tag="U3989_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1716033.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYV"
     gene            complement(938..1903)
                     /locus_tag="U3989_002618"
     CDS             complement(938..1903)
                     /locus_tag="U3989_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1716034.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(1900..3438)
                     /locus_tag="U3989_002619"
     CDS             complement(1900..3438)
                     /locus_tag="U3989_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN1716035.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
BASE COUNT         1041 a          818 c          627 g         1209 t
ORIGIN      
        1 tacataattt ccttcaaaag tggcgactgg tgtcaatttt ggggttttcc ttgaaacaat
       61 atctccacac gcaaaaattt ttggattgct cgtttgcaga taatcattga ctgcaattcc
      121 gtgtttatca aagacgatat tcgcttgctc aagtgctaaa gattctgtat tcggctgtct
      181 cccagtcgca caaaaaatca tatctgctac taattccgtc tcttttccga ctaaacgata
      241 gcgagtacct tctgaaatga tcttttgggt ttcaacacca aaagcaaact gaatgccaga
      301 tgcttccatt tgatggacgg cttcctctac caaagacgcc tcaaattctt ttaatggccg
      361 ttgattatga tggacaatgg ttactttgct ccctgcagca ttggcaatcg tagctagctc
      421 aaaagcaata taaccaccac cgataaaaat gatttcttta ggcagtacag gcaatgataa
      481 aaagtctgca ctagttttca agtattcctt tccctccact ggtaagatcg taggtctttg
      541 cccggttgcc aaaactaagt aatccgcatg gaagacttct tcgttgactt caatagatga
      601 atcatccaaa aaacgagcgg ttcctgaaag atgatcgatt tcagcttctg ctaattgttt
      661 ttttcggctt tctgggactg gatccgtaaa tgttcgtttg aatttttgaa gttcttccca
      721 gttggcagtt gggatctcgt taaaaccttt tccactcaat tgttttaccc gattacgtgc
      781 ctctactgca cttaacaaga cttttttagg atcacatcca cggttaggac aagtcccgcc
      841 ccatagatct tcttcgacca ctagtactgt tttacctgct tctttcaatc cataagcagc
      901 acttaagccg ctcacacctg aaccgatgat gattgcatca tatgttttca tttccttcct
      961 cctctcgact taaaaaagca aagatttctt tacttgtcag cgacccaata cttctttgct
     1021 tttctactat cacaatgctt ggatacaccg aaagaagcga atatacttct ttcataggcg
     1081 tatcaaagca aacttgttga cgagcatttt ctggtatttc atttaagaaa tatccattga
     1141 cgactaaatc ttctactcgc cactctttcg tctgtttcgg ttcatcaaag aaatgccgaa
     1201 cgtagtcgtt tttaggattc atcaataact gctctggtgt gtcacattgg ataatttctc
     1261 catccttcat aaatgcaata cgatcaccta gcttgattgc ttcttccata ttgtgcgtaa
     1321 cgaaaacgat ggtcgtgccc aattcttcgt gtaaggacaa aaccaattct tgtaatgagt
     1381 ttcttgaaat cggatctaat gcgctaaatg gctcgtccat caaaatcaca tctgggcttg
     1441 cagcaatagc tcggatgatc ccgatccgtt gctgttctcc tccagacaac tctctaggca
     1501 tacgatctcg ataaatatca ggagataagc caactttttg aagaagttca tctacacggt
     1561 ctgctctttt ctgcttttcc caacctaaca tttcagggat gacttcaata ttttgcttga
     1621 ctgtcatggt cgggaacaaa gcgatttgct gcagcacata tcccatattc caccgcattt
     1681 tctgcagatt atattcattt aacgattttc ctttgaagta gatgtctcct gctgatggtt
     1741 tagataatcc attgatcatt ttcaaagtag ttgtttttcc actaccggaa ggaccaacca
     1801 aaacaaagat ttctcctttt ttgattgcca gatttaggtc atcgatgaca tactttccat
     1861 catacttttt ttgcacatgt ttgaactcga ttaatttagt cactgatctt cctcctttac
     1921 taatccttca tttttcagga agattcgtgc tacttctgtc ggctttttct tttcgacatt
     1981 gacttggtaa ttcatatcaa tcatttgatc ctctgttatt tttccgctta atttattcag
     2041 tgctttaacg atttcaggat gttctttagc aaaatctttt ttcatcagcg gtgcgccttg
     2101 ataagctggg aataaccctc gatcatcttc tagagtcact agatcgtact ccttgagctg
     2161 gctatctgtg gaataggcat ccacaatatt gacatctcca ttattgattg cttggtaacg
     2221 caaacttggc tccatgcttt gtacacttga aaaattcaag ccatatgctt cttggattcc
     2281 tttatatcca tctgaacgat caataaattc cagtgtgaat cccgctttga tctgatcaga
     2341 gatagctgcc aaatcggaaa tctttttaag ttgatgttcg ttcgcaaaag atctcttgac
     2401 tgccaaggca tacgtatttt ggtaagccat tggttttaat aatgtcatat cgaattgctt
     2461 attcaataat tcttctgctt cgtcatatgt tttttctttt gtcaaatcag cacttttcat
     2521 tgcttcaggt accttcacca agctttctag gacagtacct gtgaactcag gatagatatc
     2581 gatttgacct tgatccagtg cattgaacaa aaaactcgtc ttgccaaaat tcggttttaa
     2641 ttcaacttct gtttttgtgt tgtcttcaat caattcttta tacatttgga tcaggatttc
     2701 aggttccgct cctaattttc cagcaatcgt gatggtttca tttttaaaca aggcagactg
     2761 atataaagaa acgccgccaa ataaacacac cactgctgct aaagtaatga tggaatatcg
     2821 aggtttctta tgctgcaacc agcgaatcag tccgctgaat ataattgcaa gcaaggcaga
     2881 actgatcgca ccaatcaatg tcaaaacagg tgtatttcgg tcaataccta acaaaataaa
     2941 tgtccctaat ccacctgctc cgatcaatgc tgccaaagta gccgtaccga tgattaaaac
     3001 taaggcagtt cggatacctg agataatgac aggtaatgcg atgggaagct ctactttcag
     3061 caagcgtctc attctggaca taccaaatgc taccgctgct tcttcaatag acgggtcaat
     3121 ttcagctaga ccaatatacg tgttttggaa aatcggaagc aaagcgtaaa taacaagtgc
     3181 aatcaatgct ggaacagttc cgatccctac aaatgggata agtaaaccta ataaggcaag
     3241 tgaaggaatc gtttggagca cacccgtgat ctgcaataag acttctgcca tttttttatg
     3301 attggctgcc caaatcgcta aaggaatagc aatcagtgct gcaatcagca aagaaatgag
     3361 agaaatggac agatgttgaa atgcagcttc cagcaactcg ccttttcttt cacttaatgt
     3421 ttgtataaaa ttattcatgc gaccttcctt tctgttattc tttaagtata aacatttctt
     3481 tcctttttcc caactaactt gtcttgcctt aaccttacag cacgaacaaa aaaaagaaaa
     3541 aaatacctct aaatatgctg acaaaaaaag aatccaagca acaaagtcgg atcaactttt
     3601 tggttctcgt tttttcacta cgaataccat aaaaatctat acgaaattgt cacttggaat
     3661 cagctatatc ttttttacac acacaacttt tattt
//
LOCUS       DASJON010000224         3653 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.229, whole genome shotgun sequence.
ACCESSION   DASJON010000224 DASJON010000000
VERSION     DASJON010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.229"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..716
                     /locus_tag="U3989_002620"
     CDS             <1..716
                     /locus_tag="U3989_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1716036.1"
                     /translation="NILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENPQ
                     HWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVTK
                     PFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPIE
                     GLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVGP
                     QVIQTVWGVGYKFDDSGVA"
     gene            716..2164
                     /locus_tag="U3989_002621"
     CDS             716..2164
                     /locus_tag="U3989_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1716037.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2234..2743)
                     /locus_tag="U3989_002622"
     CDS             complement(2234..2743)
                     /locus_tag="U3989_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716038.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(2733..3458)
                     /locus_tag="U3989_002623"
     CDS             complement(2733..3458)
                     /locus_tag="U3989_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716039.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1305 a          613 c          746 g          989 t
ORIGIN      
        1 tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag atgtttttct
       61 taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc gatgcctttt
      121 tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc acactggacg
      181 gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt atcatgctga
      241 cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca gatgattatg
      301 tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg catcgccgta
      361 gtgagttggc agaagcaaga gaagaagcag tagtttcaga tgatcatttt gatatcgaga
      421 ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac cggccaattg
      481 aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag
      541 tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt ggtgatgaac
      601 ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag
      661 tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt gcctaatgcg
      721 ttatttgtat cagcaactct tagctttctg cggaatggtc gcgttgatct tattgatcgt
      781 tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt accagcaact
      841 gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata cgctcccgca
      901 gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag tactgacaga
      961 acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc cgactaatac
     1021 agctttactg aattttacct taacagctga tcaatggaaa aatctgagga atggacgtca
     1081 agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct cttacgcact
     1141 tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc aacctgcgcg
     1201 aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat ttattttttc
     1261 cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta agcgtatcaa
     1321 tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac agctccccgt
     1381 acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga cgaattcatt
     1441 gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga
     1501 tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg aagggttaga
     1561 atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga aaaatgaaac
     1621 agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca
     1681 aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca
     1741 gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg ttgatgatat
     1801 tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc
     1861 gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct atcttgaaac
     1921 gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga aaagtatctg
     1981 ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg aatcaggatt
     2041 aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag aagtagaaag
     2101 cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaga
     2161 gtaaaaaaca agcggtataa aagatcatga atcgatcttc tataccgctt gtttttacta
     2221 taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt tgagcaatag
     2281 tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa taagaaagca
     2341 agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct tcaataacaa
     2401 tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt tcaaaagacg
     2461 tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt tcttgatagg
     2521 ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg tctttaagat
     2581 tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc agttttgcaa
     2641 caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga ggacacaaaa
     2701 taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc aggcctccta
     2761 ctttgcaaat gatggataaa aaaagtgaaa gggaatagac taagaatgag acagcatcca
     2821 ctgagcccaa taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt
     2881 tggaaatcga tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa
     2941 gtcaagatat cacggatatc tggcagttca gtagtcttta agacaagaaa attgatcctt
     3001 tgaagaagcg cttcataaac tgactggaag cttacgatcc acaataaaac caagatacaa
     3061 caaagaaaga gaggaaagaa cagctgccag aaaataaaaa ggaataattt ggttttagaa
     3121 agaccagtca ggtgccaatt gatcaattcc tgcttgtttt gcctgactga atagtaaaag
     3181 aaaaagataa aaaggaaggc aaatagaaaa aataaactga atatgccttt tatataaaat
     3241 cggctagcta attctaaagc cggaagaagc tctgtagatg cagaagcgcc aataaatgaa
     3301 agccgatctt ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt caggcatgct
     3361 atcaataaaa aagcagaaaa gcttgaaaat aaacaataga gtattgtctg ctttttgtaa
     3421 taaattaatc gagcagtagc atagcggatt attttcatac ttttcacctc tataaaaagt
     3481 atagaaaatg aatatgaata aagtatgaaa ttcctttagt gaaatcacga attaaaacca
     3541 gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg ccctattcta ttaccgtaga
     3601 tgcaagcttt tgaacactgt ttattcgaaa agaggagcag cgacaataat ttt
//
LOCUS       DASJON010000225         3628 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJON010000225 DASJON010000000
VERSION     DASJON010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.230"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            293..1138
                     /locus_tag="U3989_002624"
     CDS             293..1138
                     /locus_tag="U3989_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1716040.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1419..2639
                     /locus_tag="U3989_002625"
     CDS             1419..2639
                     /locus_tag="U3989_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN1716041.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2636..>3628
                     /locus_tag="U3989_002626"
     CDS             2636..>3628
                     /locus_tag="U3989_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1716042.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEW"
BASE COUNT         1352 a          535 c          674 g         1067 t
ORIGIN      
        1 ttgtttaaat cttttgctaa gttgaactaa tagagtcttt tatgaatatc gaagttagtt
       61 tttcacctaa cttcgttttt tatagttggt tattataaaa tagtaatata gctgatttgg
      121 attcaattca tatttattta taaatcaaaa atacagagaa tagtatataa atgtcctttc
      181 ctattcttaa aatgatgttt gtacttactt aaattaggta taattagtat atgttttgtg
      241 agaaatttag acttacaata agcagtgaat ttataatcaa cgtataggag acatggatga
      301 aatgaaacta attggtatag atttagacgg tactcttttg aattctgacc agagcattag
      361 tcaaaaaaat gcaagaacaa tgaaacattt accggaagac tgttttccat tcatttgttc
      421 tggaagagaa gtagaagaca ttaataatat tctcaaaaaa tctggtttaa ggataccagc
      481 agtagggtta aatggttctg ttggatttga tggagaaaaa aagatatttg agtcttcctt
      541 cgataaaaat gatgtaaaaa aaattgattc tgtcctatcg aaatttccaa ccaaaatata
      601 tacaaatctc ggaagttatg aatctcagaa ttataaaaat aaaatgaaaa aaatttttca
      661 cgaggtaggg agtgaatttt ctatcaaaga gctaaactat gagcttgagt atgaaaaatc
      721 tatttgttca atcccttata ataatattga ggaaattata gaaaaggatg ctatcagcat
      781 ttacaaattt tttattttta ttcctaatcg tcaattgaaa gctaatatat ttaaccattt
      841 gaaaaagatg ctaaatgtgt ctgttactga atcggcacct gtaaatattg aaatcgtacc
      901 tcaaaatgta tctaaaggtg tggtgtttga tcgtttggaa tcaatctaca atttaaagaa
      961 acctttgaga atcgcgatcg gagatagttt aaatgactta tctatgtttg aaagtgcaga
     1021 tattagtttt gcaatgggaa atagtcatca agaaatcatg aaaaaagctg attatgtcac
     1081 tgtttcaaat gataaagacg gagtatctca agcatttgaa agaattctta ccatataaac
     1141 taattaaggt agtatagaac atagaattct aaatacggaa aaatggagca taaattccag
     1201 tacaaatagt acttcttcag tgtcaaacga agcgaatata acatagatat ttaggattgt
     1261 gacaaaaatc atgtcacaat ctttttttcg aataaactta ttttttgaag tcggccattt
     1321 atcgtaattt ctttgcataa tacgaacaaa ttttcggtta ttgatgaaaa aaaacattga
     1381 aaatggtatg ataaaagaaa aaaaggagga aatttttaat ggtgaaattt atccatgcgg
     1441 cagatttaca tatggatcgg tcatttgaag gactgacaac tcttgataaa actgtacagg
     1501 aaaaactatt gaagacgaat ctgaccgttt tatcaaatat cattgaacag gcaataataa
     1561 ataacgttga ttttgtttta cttgcaggag ataattttca tcaaaatcgt ccttcattaa
     1621 aaatacagaa acatttttca gaacagatga aacggttaga aaagaaccat atacctgtat
     1681 ttataatatt cggaaatcat gatttctatc aaaaagagcg gtattggttc tcctttccta
     1741 aaaatgtcca tttatttaca tccgaaacgg tagagacaaa aaaaataacg ataaaaagtg
     1801 gagaaactgt ctctctcagt ggatttagtt atcgtcaacc gtggattcaa aaagacaaag
     1861 tgatggaatt tccttctaga gaactcacag attaccatat tgggttgtac cacggggaac
     1921 ctggcgtctc tcaaaaagga aactatgctc ctttccaacc ctcaaagatg caagaaaagg
     1981 gatacgatta ttgggcttta gggcatatcc atgtgccaac ggtcttaaat gaaaagcaaa
     2041 caattgttta tccaggtgcg cctcaaggtc atactaaaaa ggaacaagct tctacatcta
     2101 ttctactagt tgaattaagt aaaggaagtt gccaaataca tccgattaaa gtagcagagg
     2161 tctattggaa gacaaaggaa atatcattga ggaaagcaag aacgacaaaa gaggtcattg
     2221 ttcacattcg tcagcagctt tcaaaagtag aggatggatt tagtttaatt gagataaaat
     2281 taaaagatta tgaccatctc aatacagatg ttttggaacg gatacaatca ggagagctat
     2341 tagactattt gttagaggaa ttctcagata ggataaatga tttctttatt tggcgtattt
     2401 ctctcataga gaatactttt tatacgaaga ccccacttgc tgcttctgct aaattgatgg
     2461 aacagttatt tcaatactat cagacacctc aagatttcca acaaatttta aatgaagtat
     2521 atagtcatca agaagcggct agaatattga gcgaactgcc ggaatatcaa gaagaaacac
     2581 ttgaaaaagc caagcagctg cttaatcaag attttttatt tgaggaggat caggaatgaa
     2641 gattttaagg atcgagattg ctgcttttgg aaaatggcgt caaaaaagtt ttgactttta
     2701 ttcaggcaat cagctgatct atgggggaaa cgaagcagga aaatcaacga tttatcaatt
     2761 tattcaagca atattatttg gtttcccttc aaaaggccgt aaaaaaaagg attatacacc
     2821 aaaagacggc tcagcctatg gcggcaaaat atggcttaaa catcctgtat atggtgaatt
     2881 tgctgtcgag cgctacaagc agcaaaatcg cggaaaatcg aaggtgtggc tgggggatca
     2941 agtgggaagt gatgagttac tagaaaaact gatcgcacct ttgacgaaag agttattcca
     3001 gcaggtattt actttccagc aagagcagtt gatggaacta gatcatttga gagaaaaaga
     3061 gctacatgat gcgctgattt ctttaggcat cacgggcagt tctcaagtat tccaaaagcg
     3121 gcaagaatat tatcagaaag cacaacaatt attcaagaaa aaaggccaaa aattactaat
     3181 caatcaaaaa ttaaatgaat ggaaagagct gcaagaaaaa attcatcaaa aacaagaaca
     3241 ggaagcttat tttggcaagt tgatccaaca agaacaggaa tacgagtcgc aacagcagca
     3301 gttattggca caattaaaag aggcgaacaa acaattttca ttagccagac agcaagaact
     3361 taatttttct ttatatgaag aatggcaatc tctcaaaaaa aacagcacta caggtcttcc
     3421 tgatgagcaa cttcttttag atctggagtc tttctccaaa cggtatcagc aaacaaatga
     3481 gcgtttggaa gaattagaag caacactgga aaagcatagc gggatggatc agcagtcagc
     3541 cagatattat ttttatttag agcaggagca ggaactaaaa gatctccagc aacttcaatt
     3601 tacagcggaa caattaaacg aagaatgg
//
LOCUS       DASJON010000226         3606 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJON010000226 DASJON010000000
VERSION     DASJON010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.231"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..191
                     /locus_tag="U3989_002627"
     CDS             <1..191
                     /locus_tag="U3989_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1716043.1"
                     /translation="LVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVT
                     VYEGHFVIAFKSGIEMEI"
     gene            334..558
                     /locus_tag="U3989_002628"
     CDS             334..558
                     /locus_tag="U3989_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1716044.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            573..1442
                     /locus_tag="U3989_002629"
     CDS             573..1442
                     /locus_tag="U3989_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1716045.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1423..2157
                     /locus_tag="U3989_002630"
     CDS             1423..2157
                     /locus_tag="U3989_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1716046.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2190..3098
                     /locus_tag="U3989_002631"
     CDS             2190..3098
                     /locus_tag="U3989_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN1716047.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3095..3575
                     /locus_tag="U3989_002632"
                     /pseudo
     CDS             3095..3575
                     /locus_tag="U3989_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1252 a          521 c          789 g         1044 t
ORIGIN      
        1 ttttagtaga agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat
       61 ttatccgcaa gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa
      121 tccagagcgt tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa
      181 tggaaatatg aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc
      241 ttttgcttgc tgattataat aatattttaa taactattgt gtttatcaac caaatggttt
      301 ataatattct tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa
      361 ctgtgtgagc aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca
      421 cagaatttca ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt
      481 gctgatgtgc taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata
      541 aaaatatcta acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa
      601 cagagaaatt atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa
      661 gaggcaccca tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat
      721 ttgacctgac agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc
      781 ttgttgtgcc tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa
      841 atgggtatca tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag
      901 atacggagca aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa
      961 gtgcaatgta tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct
     1021 tatgcgaatt aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga
     1081 acttttttat atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag
     1141 aggataaata ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac
     1201 tatttgcatg taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa
     1261 cttttgaata taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg
     1321 gtctttctct ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa
     1381 ttgtatcgga gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat
     1441 gatgataata tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct
     1501 ggtgcgggag aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg
     1561 cttgatttag gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc
     1621 tctgtagtag gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat
     1681 tttccacaga ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc
     1741 tttgatgtaa tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata
     1801 aaaaagatat ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct
     1861 gtttttactg ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat
     1921 ttcccggtgg acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag
     1981 gttacaaaat atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt
     2041 ataataaatc agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg
     2101 gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa
     2161 tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag
     2221 tactttcttt agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg
     2281 caaatattaa tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg
     2341 atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga
     2401 tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta
     2461 tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata
     2521 attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa
     2581 gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg
     2641 atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta
     2701 aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa
     2761 tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata
     2821 agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga
     2881 tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg
     2941 tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa
     3001 caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca
     3061 catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca
     3121 cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag
     3181 gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag
     3241 ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa
     3301 tactgataag ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg
     3361 ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact
     3421 tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata
     3481 attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
     3541 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
     3601 tcgtct
//
LOCUS       DASJON010000227         3586 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJON010000227 DASJON010000000
VERSION     DASJON010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.232"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            71..3349
                     /locus_tag="U3989_002633"
     CDS             71..3349
                     /locus_tag="U3989_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN1716048.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1247 a          654 c          791 g          894 t
ORIGIN      
        1 attgacagta aaaataaatt aggtaatact ttaagtaaac gctttctaat ttgttacggg
       61 aggaatacac ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga atctccttat
      121 gctcagtacc ccggttttaa tttctgtcgt tgaagttgct gctaacgaac aacagtctgt
      181 gaacgaagaa aatgcacacg tgaacattga agggatgtca acaatggagg aaataccaag
      241 aactagtgaa gatatggata cggaagaagg aaatgatcct atgatgaaag aatcagaaaa
      301 tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg ttgttttcgg
      361 agaaagcaca agtgctagta ggtatgccat tgaaccatta gaagacggcg tgcggctctc
      421 ttccacgaat aacggcggaa aattccagtc aagcggatca gatggaatga cctattatta
      481 tcaagcaatc cccaaagaca tcaactttag gatgcgggcg acgatcgaaa tagaatcttg
      541 gacctatacg aatgcccaag aaggatttgc tttaatggta agagacgcag taccaaaaaa
      601 tcatttgttt gggcaggcat tttattcaaa ttcatttgcc atactaggca gtcggatcga
      661 atatgtctgg gactcggata gacaagaagt ggtcaacacg agcggcagca aatatacgat
      721 gcgacttgga ttggggacac gggcaatcac aggaatatca tctgaagatc cgcaagctgc
      781 gccagctcct gggagtgtct cagtggaaac aacaccttta gagacttcag cagggttctt
      841 ggaaaaagaa acaggttctt ataatatatt cggaaacggt catgggaatc tgtttccggc
      901 cacgaactcg attactaaac tggacgtaga gatgaaaaaa acgaatactg gtttagaggc
      961 atattattac gatcctgaaa caggagaaga aatggcttct gacatcatgt atgactggga
     1021 aaaattatac gcttcagatg aatcggttat ttatgcagga tttgctgcag caaggaatat
     1081 gacgatcaag gtagaaggaa tcgaagttgc ttactcagat cctgcgacag atcctcctgg
     1141 gcaagaaaga cctactcgct tgattgatcc gatttacact gtgacctcta gtaatcattc
     1201 aggaaaccaa gatcatacta tgtcctttag agcaaatgct gacggattat tgacaattaa
     1261 aaataaagca acaggagagg tgcttaagaa cgcaaaaaac ttggctatca ctgcgaatcg
     1321 ggaatttgat tatcagacaa aactgatgga aggaacgaac gaatttactt tcagttttac
     1381 gccagataaa aactatccgc cagaagaata cgtagagatg actagttatg aaacaaaaga
     1441 gattcttcat acagtggact accgtaaacc taaatattcg acagtttatg taacaccaga
     1501 agggtcagat gcaggaaacg gaagcagaca aaatccatta tctctgacac aagcattgag
     1561 atacgcatat gctgggcaga caatcgtcat ggcgcctgga acttattcct tcgaaagagg
     1621 attgacgatt ccaaaaggcg ttaacgggac aagagaaaac ccgattcagt tgattgcaga
     1681 gaaaaatcca gaaaacaagc gtcctgtact tgattttaaa ggaactggaa atgggatgac
     1741 actgttcagt cactactggg gtttacgagg gtttgacatt acaaatagtg cagctatgca
     1801 aaaaggatta cagatttctg gaaatcataa tttgatcgaa gagattcatg cttatcggaa
     1861 tggaaataca ggaatccaaa tcagcggtag ctcaaatgat cctttcgaag catggcccgc
     1921 gcataactta gtcaagaact gtacatcttt tgaaaatgcc gatccaggat ttgaagatgc
     1981 cgatggattt gcggccaaac tgacaatagg agaaggaaat gtatttgacg gttgtatcgc
     2041 gtatcataat gcagatgacg gttgggacct ttttgctaaa aatgaaacag gttcgatcgg
     2101 aaaagtaatc atcaaaaact cagttgctta tcgaaacggc tgggtaccag gaattgaggg
     2161 agaaggtaac ggtaatggat tcaaaatggg cggttcttct ttaacgggcc ctcatgaatt
     2221 gatcaatagt ttgtctttcg aaaatcttgc gaaaggaatc gattccaata gcggaacaga
     2281 tattatcgtc aatagcagta caagttttaa taacggttca tataatgtgg ctctttatac
     2341 ctcttcagca ggaaacacag actaccatgc ttctggtatc ttatctttcc gaacagagaa
     2401 tcttgagatg agagagcaga tccgcccatt gaatcaaaac gaagatcaaa tctaccactc
     2461 gttgaactac tattggaatg aacgagagaa acaatcagaa aatactcttg gacataccat
     2521 atcaaaagat tggatcgagt ctctaaatac atgttcgaaa gaaggtcagc agcctgttac
     2581 gcgctatgaa aatggaagta tccatgttca tggactgatg cagattaaac cggggaatcg
     2641 tcaaacagaa aaggaaagag tggatggtct tccattatct gtaggtgcta tttttgacgg
     2701 agaaacctca ccgtcttcgg aatatccaga gtatgcaatc gtacaagaac caaatcagac
     2761 aaaaccaaat gaacaaaata atccgattat tatacaagaa gcaaatggtg tggcggagaa
     2821 tgcagaagga aatatcattg aactgaagcc ttttacagtt tttgtgtcaa ctttcacacc
     2881 aaatcaagga acgcctgtct ttgaagatat gcgttatttc ccagaaaaga tccaaatcgt
     2941 tgaaacacag tcaagtgctc aattttctaa tcataccgct attttttcag caccttcaaa
     3001 aggagaatat acgctagaag tcacttatcg ttcggaaata tttgatgggg aaaaatggtc
     3061 tgctcataca gaatctgtta cccagcaaaa agcgatcact gtgcgagaaa ctgccgatgt
     3121 gcccccggga aatgacaaac cagaaagaga aaatccagga aatgaatcac caaatggaaa
     3181 accaaatgga aaaccttctc atccggatag gacagcaaaa gaaagtgaaa aaaatggcac
     3241 tcaaggactc ccaaaaacgg gagaatttaa taacccgttg ctagcattag ctggaggaat
     3301 tttgttaatc ggtgtagttg tttatgtcat gaaacagcga aaaaaataag tggaatttct
     3361 ccttttatga tgtaaaacaa ttgactgagg aaagtaaaaa tagaaggaaa tgagaccaat
     3421 agatgtctca cttccttcta tctttttata gatgaggttc tccttgctgc ttttgattgg
     3481 ctgttcttat tacttgatgc aggacagctt tttcacaatc tctccactaa acggtgttca
     3541 atcagctgac cctcggtatt agctcaacaa acggcaaaat atgagg
//
LOCUS       DASJON010000228         3574 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJON010000228 DASJON010000000
VERSION     DASJON010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.233"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(278..703)
                     /locus_tag="U3989_002634"
     CDS             complement(278..703)
                     /locus_tag="U3989_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716049.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1077..2048
                     /locus_tag="U3989_002635"
     CDS             1077..2048
                     /locus_tag="U3989_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1716050.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2080..3477
                     /locus_tag="U3989_002636"
     CDS             2080..3477
                     /locus_tag="U3989_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1716051.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT         1229 a          584 c          742 g         1019 t
ORIGIN      
        1 agttttgctt ggatttgttt atcttcttct actttttaca taattttgaa caggataaga
       61 ttcaaatgtt ttaaatgaat ctttgactag tttgtgacag ttgtcgcata tgctcaagct
      121 tccttggtca ttaaattctg tatggatatt tttgttgtta ttacatgtac aataatcagg
      181 actatcgatc atccatttcg tctcctttac aatcatcttg cttctttact atagcagaaa
      241 agaaggggag aaacaaaaga agatccaaca gacggcacta tagactgtag atgttgccat
      301 cctcatcctt caaacggtaa ttaccgattt ttgtaaatat gactgttgtt tcttttttat
      361 tttcttttcc tgaaataata tattcgtcat ctttggtagt gatcttgata tctgtcagca
      421 ctgttttttc ttcagatgat tcagaagaat cagttccttg tgtctgatac gtgcgtgtcc
      481 attccatttt tttatctgta aatgtcaact gtcccgcgga taaatccata tctttacgta
      541 cttttagcgt atatacactg ttggtggtat ctgctttttc cgcttttaag acagaagaat
      601 cggttgtttc gtccgaggat tggtcagagc cgccaaacaa atcacagcca gtcaatgtca
      661 aaagtcccgc agacaaaata aatagtacga ttaatttctt caaaatactt cccccatttg
      721 ataggataaa gaaattatac cattggaatg taaaacttca aacggatgga agagaaaaac
      781 gcacaaaaca cccacaaata gagtaaattt tagaataaaa ttttaaaaag gcaaaaaatc
      841 cgccactttc agaaagcgtt ttaaaaaagt gttgcaatct tttaaaaaaa tgctatatta
      901 aaaatgaacc ggttcaaata cttgtttaag tataattaat agtgagagga agttgctcta
      961 ctgaagactt tagttatatg gatattgatg acaagcaaca ctatgcaaag aatgcggtca
     1021 tcataatcga acaacaagaa agagaaatca aatgtaactg gcaggagtga gaatgtatgg
     1081 catcgatccg tgatgtggct aagatggctg gtgtttcagt gggaacagtt tcccgttact
     1141 tgaatggtca gcaactgaag gaaaagaaca tgaaaaaaat agcagaagcg atttcagcgt
     1201 tggactataa agaaaacatc attgctaaag ggttgaagaa taatcgaagc ttctctattg
     1261 gacttttgat aaacgggatt tccagccgtt ttgggtcgga agttgtctca gggatcgaaa
     1321 gagtagctga agaaaatggg tatagcttat tattaagcgg gtttgccgat cagccagaaa
     1381 aaattgaaca aaaaattgaa tacctgatga agcatgtgat cgatgggctg attgtcttct
     1441 tatccgaaga agagtggtct ggatttgaaa tgctcacaaa aatgagtatt ccagttatcg
     1501 cattgaattg tccagaagga ccagcaggta ttgatacaat attagtgaat gatcgagaaa
     1561 gtgtagcaaa agtgatcgct catcatggtg aacaaggaca taaaaaaatc ggatttattg
     1621 ctgccactca aacagactac gtagcaagag aacgtttagc aggagccaaa gaagcggtcc
     1681 aaaatgatcc atcgatccaa ttagaggtct ttacaggaga ctattctaga aaaagcggct
     1741 attacggggc aaaagcatta ttagaaaaag gggcaactgc tatttttgtc agcaattaca
     1801 atatgtcgat tggtgccatc gaatgtttca atcaagaagg tgtcagaatc gggaaggata
     1861 ttgcgttcag tcactatgat tatttagata aaaacgatct gagtatcctt ccgaaaatca
     1921 cgatcacgcc accaacagat aaaatcgggg aacgagcagc agaacagctg ttaaaacgaa
     1981 tcaaaaaaga agaggaagaa aatgaaaaag tgattgtgat ggaaaatatc atacatggaa
     2041 tcaactaaaa cgaactaaaa aagaaaaaga ggtgctttga tgaaaaaata tcaatttcca
     2101 gcaaattttt ggtggggttc tgcagcaagt ggcccacaaa cagaaggaag gatttcaggc
     2161 gatggaaaag gagagaacat ttttgattat tggtacaaaa aagaaccgca aaagttcttt
     2221 gatcaagtag gacctgaaaa aacctcgcaa gtttatacga agtatcaaga ggatgtacag
     2281 ttgatgaaac agactgggca taattcattt cgaacatcta tccaatggag ccgtttgata
     2341 cctgatgaat ctggaacaat caacgaagaa gccgttcgtt tctatcatcg ttattttgat
     2401 gagttgattg aaaacggtat cgaacccttt gtgaatctgt atcatttcga tatgccgatg
     2461 tatctgcaag aaaaaggcgg atggttgaac cgagaaacgg ttgaagccta tgaaaaatat
     2521 gccaaaagtt gttttgaatt gtttggttcg aaagtaaaaa aatggtttac tcataatgaa
     2581 ccaatcgttc cagtcgaagc aggttattta tatcgatggc attatccaga agagtcagat
     2641 atgaaaaaag cgatccaagt tggctatcat gaagcgttgg cttcagcgtt agccatcaaa
     2701 tcttaccatg aaatggagca aggtggtgtt attggtatca tcttgaatct gacaccaagc
     2761 tatccaagag acgaatcaaa ccctgaagat gttaaagcag cgcaaatagc agatgcgttc
     2821 ttcaatcgtt cattccttga tccagctgta aaaggcgagt ttccaagtga attgattgag
     2881 atagtaaaag aacttgatct catgcctgtg atccatgcag aggatttgac ggttatcaaa
     2941 gaaaatacag tagatctttt aggtatcaat tattaccagc cacgccgggt aaaagcaaaa
     3001 gaaacaccaa tcgatactaa aaacggtcca atgcctgagg attatttcga caattatgaa
     3061 atgcctggtc gtaaaatgaa tccttatcgc ggatgggaga tctatgaaca aggaatctat
     3121 gatattttga tcaatgtacg agatcattat ggaaatatcg actgctttat ttctgaaaac
     3181 ggtatgggtg tagaaaacga agcacgattt atcaaagaag atggcatgat ccacgatgat
     3241 tatcggatcg aatttgtcca atctcatctg aaatatgtcc atcaagccat tcaagaaggt
     3301 gccaattgtt taggctatca tatgtggaca tgtatggata actggtcgtg gaccaatgca
     3361 tataagaatc gatatggctt tatttcagta gacttagcac aagatggaaa aagaacaatc
     3421 aaaaaatcgg gtcattggtt caaagacgta tcagataaaa acggatttga ggcttaagaa
     3481 agaatagttg agagcaaatg cgtgaaattc tccattctta ccattttgtg aaaaagaagt
     3541 tcaattattt acgatctttt tcaacttttt tgtc
//
LOCUS       DASJON010000229         3571 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJON010000229 DASJON010000000
VERSION     DASJON010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.234"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..651)
                     /locus_tag="U3989_002637"
     CDS             complement(<1..651)
                     /locus_tag="U3989_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1716052.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELF"
     gene            complement(678..1268)
                     /locus_tag="U3989_002638"
     CDS             complement(678..1268)
                     /locus_tag="U3989_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEN1716053.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(1586..2647)
                     /locus_tag="U3989_002639"
     CDS             complement(1586..2647)
                     /locus_tag="U3989_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1716054.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(3215..>3571)
                     /locus_tag="U3989_002640"
     CDS             complement(3215..>3571)
                     /locus_tag="U3989_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1716055.1"
                     /translation="TGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIA
                     TDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDG
                     NFFDRILNLYQKGSKE"
BASE COUNT         1201 a          685 c          386 g         1299 t
ORIGIN      
        1 aaataattca ggaataaaaa ctttcaattc agctaatgca gacataaaaa gtgctgcaca
       61 aacacataaa ataaaatgga tgaaattttt ttctcccttg aaatatccga ttgtaacaac
      121 agtaactgtc acacacaaaa ttaaattcaa tggcatatta caacctgaat aattccaaaa
      181 aactccacct atcatatctc ctacaattgt tccagttgga taggtgacca tgaatctttc
      241 aattaaacaa agtggcagtg aaatccagaa taatttttca attatagcga atactttata
      301 gtaatcttta attgttaaat atgttgcaca atttatatat actatgaaca tacgagtgtt
      361 atttcgtatc ccccagataa tcaatcctat attttctaaa ttaataactg agctacaaat
      421 agaaactagt aaaaacaaaa ctatcagcat ctcatttttg ctagcatata ttccttttct
      481 gcgtctaaag ctaagtgcat ttaataacac aaagatatta attatatctc ccaaatataa
      541 aattgctctt ggggcgttaa aagtatatac aagaactatg gagattgcaa aaaatgccaa
      601 cgtgaacaat gtccaatctg ctcctgacca tctttttatt gaaatacgca tcgttattca
      661 ccttctttcg ctaaaaatta ttctaaaaat acttttgtta aattaacata ccctattatt
      721 tttgccggta cacctgcaag aattgcacca ttctctagac aatctttgat aacaatagct
      781 cctgccgcaa caactacatt gttagctatt tttacattac caataatctt tgcacccacg
      841 catacacgta cattatttcc aattacagga cacttaggat cgattccatc atttccaata
      901 cagttattcc catataagta acaatttttt cctattatgg cgttaccatt aataacaatt
      961 ccttgtgtat ggtaaaatct tacaccttca tcaaatacat tttcaccaca ctctatacca
     1021 cattttcttc ccaaactatt cattcttctg caagacacaa tatacataat cgcataaatg
     1081 atatttttct ttctctttga ataataataa gatgtaattc tcattcgacg agtataccgc
     1141 caagcgttgt agtccggatg tgatttcatt atttttagta attttgtgtg ttgtccttta
     1201 gattctatat atagattctt ttccatacga agaaccgctt ttaaatattc tttactgcta
     1261 atttccattc atatctccta ttaattcaat ttatacttaa caattttatt cggatttctt
     1321 gcaacaagag catcattcaa tgctactgaa tctacaacga tggatatgtt atcttcatcg
     1381 ttgattccac cggatacaat attacttata aaaatcgtaa cattttttcc aaaagaaggg
     1441 atgtccgtga tgtataatgc ccattttccg atatttccag ttatttaaac ctaacgaatt
     1501 tttacaatag tacaagtatc acaatcatta atatataact ggaatataaa tctccaaaaa
     1561 ttcactatag tacagttttt tatgattaaa atctcctatt tctacccatt ccgcttaaat
     1621 atgctaatgt tttaacccca ataatggatt ggtttagtaa aaaatagtta aatattcgtt
     1681 gtttaattgg attttgttta tatgggtatg agtgaataca ggcaatcaac tcatcgttca
     1741 taagtatctt agacaaatag gagtttaaat caaattcttt attaaaacta gcatatctaa
     1801 ctgccttttg aatacaagta cgacagcgtc ccaacaaaag tctccttgtt ttgtttacaa
     1861 atatctcatt ttctaatata tagcttaaat cgccttcaat ttttttaact aattcaatat
     1921 tacaatcgaa ctcattatct ctaaaattat gtgtcaatga attattatta ttacatctat
     1981 aataatagat acaatcttta ataaaacttg ttatttttga atgttgcaaa tatgaaatat
     2041 ggaatatgat atcttcagat atataatccc tttctgacgg aaacttgata ttgtgctgtt
     2101 gaattatctc agatgaatac agcgcatgcc aaactgacat tggcaatata aaatcgactt
     2161 tataactcgg ctcagtgcca atcattccaa gcaacacttt ttcaattata gcgttatttg
     2221 taaatatcgt actatcatat cttgctggct ttgaaataat attattatca tcataatatt
     2281 cataatatcc agcataacat gtgtctgcat tactcctttt tataccattt aacattgctt
     2341 caatataatt agctgcaaca aaatcatcag aatcaataaa agcaacatat tttcctttta
     2401 catgttcaag tcctgtattt cttgccaatc ccaagccagc attattttta tgaataatac
     2461 gaatccttga atcctttaat gcatagtctt cacatatttt attagatgaa tccttagatc
     2521 catcatccac aattataata tctaggttct tatatgtttg attacacact gattccaaac
     2581 aacttcctac atacttttca acattatata caggtatgat tacactaatt agatcactcg
     2641 aattcatcct tctatccttt cactcacgtt ttctcaatta gtcaatgacg gcatcaacga
     2701 ttttcttaaa ttctctgaaa aaaatagact ctgatcttga tgatgtgaat gacatactat
     2761 cttcttctag aaaaattatt tcctttaaat tatttattaa atcaataata tgatcctcgt
     2821 aagtatgctt tcccttggaa taatgaaaat atacttttgt attacacaat attgcaattt
     2881 tatttcttaa tatgattatt tcactcattc attactgcca catatacaca acttataacc
     2941 accccctgca aagtttaggg taagttatca gataatatct tcctcttcat catccagaat
     3001 acaaaatcta tttatttata gaaatatcat taatgttttt atataatgta ccgtgcttgt
     3061 actcttgctc tattacaagc actaaaacac tactattcat ttgaattctt acacggataa
     3121 aaatttacat cgtctatttc aatatttttt ctctctattt tattcactta ataagttcat
     3181 tacataatcg aaacattttt tattttcctt gtcatcactc cttgctccct ttttgataca
     3241 gatttaaaat tctatcaaag aaatttccat cgttaaattt cgttattgcc aaatccttcg
     3301 aacaacgtga aaattttacg tatttatctt tatcattgat ttttagtatt gcagatgtca
     3361 acgctgatac gtcaccaggt tgaaacaaat atccattttc tccatcacgt actgctgaag
     3421 aaatatctcc tacatctgta gcaattacag gcattccata agaaatcgcc tctagaatag
     3481 atataggcaa tccctcgtta taggacggta aaatcatcac atttgatctt ctatagcact
     3541 cctccttttt ttctcccaca acccaacctg t
//
LOCUS       DASJON010000230         3539 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJON010000230 DASJON010000000
VERSION     DASJON010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.235"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(328..543)
                     /locus_tag="U3989_002641"
     CDS             complement(328..543)
                     /locus_tag="U3989_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716056.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            650..964
                     /locus_tag="U3989_002642"
     CDS             650..964
                     /locus_tag="U3989_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1716057.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1068..2246
                     /locus_tag="U3989_002643"
     CDS             1068..2246
                     /locus_tag="U3989_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1716058.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2649..2942
                     /locus_tag="U3989_002644"
     CDS             2649..2942
                     /locus_tag="U3989_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN1716059.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1201 a          539 c          668 g         1131 t
ORIGIN      
        1 gatacttgaa tttgatacaa ctcgttaaag tattttttcc ctttcctttt cattctatgg
       61 aagtttttta tcgggtcaaa ataaaggatt tccataggct gaaaatagcc tagcgctctt
      121 tgacaaatga atagcaaatc tatcagtaca tgtagatttt gattctctag ttacgccatg
      181 acaaggttag cttttaagcg acaaatttta atatgagaat cgcgactacg ataatgatac
      241 ttccgtaacc aaatcggccc gccattactg gggggaggtg agattcctat gtgctttggc
      301 aaaacagctg atagatcaga aaaactacta tttccatatt tcgattttgg ttggcatatt
      361 tttcatgcca aattctgtga ttttaatagc atcggcaaga tttaattttt cttcagctct
      421 ctctccactt ttaagttttt ttactgtact caagttgatt cctgttccga atgctattgc
      481 attagccgta gatttttcaa gaatttcttt aattattttt atgtctgcaa ttccttgttt
      541 catattaaaa attcctcttt tctagtccgt tttgatggag tactggagtg tttttttaca
      601 attttaaggg gtaccgtggt ggtcgttttg attttgagag agaagggtga tggagttgga
      661 agttaaaaca aatgttaatt ctctggaaat agagttctat tcaattttac aaaaaaaact
      721 ggaagattac acggaaaaat tgcttgcagt cagtcattta cctctggtat taacaaatac
      781 tgatttaaag cgactatttc agataagtga tagtacactg aaccgtttag taaaacttgg
      841 tgagtttcct ccctgttggt acgggattcg aggacactat tttcgtgatg atgttttaga
      901 atggatgaga aagagtgatt cgaatgacta tcgagaaaaa ttgcgactac ttcgatcttt
      961 ataagttaca ttttctttct aagatgcggt acaatgatga catagataga aagatttcca
     1021 aagggtttct gcttgttggg ttccttcaaa ataggaggaa tcaataaatg gaaaaaacag
     1081 atcgattcaa gaagcaaatc agttctactg gtaggatcac atggtacttt caagcatatc
     1141 gaacaacaag acgtggcttt aattcaaaac gtgaggcgca actcgcttat ctggaaatgg
     1201 agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa tgacaagttt agtgttgtag
     1261 gtgagcaatg gttccaatat taccgttcgt taaatgaaca aaaggaaata acctatgata
     1321 agcgagagga acaattaaaa attattgttc gatggattgg tgataaaaag ttgtctgatt
     1381 tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa ggaatgtggc gttaatggaa
     1441 caaatgttgg ctatgccaaa aatagtttac aatcacttat tcaagttcta aatatgattt
     1501 ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc aatgaagtct gtgagaatgc
     1561 caaaatatca gttaacggta actgatttaa aagaagccat aaataatatt gaggacaagt
     1621 ttcttactgt cgatgaattg agaacttttt tgaattatgg cattgttcat gaggaattac
     1681 cgatgtctgt acttttccat gttctatttt acactggctg ccgagtcagc gaggcgcttg
     1741 ctctacagcc tggagatata gatttcaaaa gaaatgaaat tctgttctat aagcaaacgg
     1801 cagttaaagg taagagtaag gattttcgaa ttgaaactac taagacagtt agttctgcta
     1861 gacgagttct agtaactcct ctagttatgg aaaagctaca acagttgatt aaggctttgg
     1921 gtgagatgag gcaacattac aggtttatta tagaagaaac atatttgttt gtgtatcttg
     1981 attctagtaa acgtggtgtt ccttatcgtc gagagtatgt aaatgatcac gtcaaacggt
     2041 gcgtgaatcg ctcaggaatt aataagccat ttcatactca tttagctcgt catacaatgg
     2101 caagcttagt agcggaacac tgtagctggg aagtcttgaa agatcgctta ggccatactg
     2161 accaaacaac ttcgaaaatt tatcgccact taacgtcaaa tgaaaaggta aaacctctga
     2221 ttgcttttag ttctttagag agttaaaaaa acggtgaaag cactggtatt acaggattct
     2281 tgaaaaacga ttttttaaca aattagtcaa tatgtagtca gaggtaataa aaatcctttt
     2341 atatcaatac tttaacgact tttcacaagt atcaaagaaa tccgtcgtta atcattgtta
     2401 tcagactgag aaagaaccct tggtattcaa gggttctttt ttcttagcat gtaatttgaa
     2461 caccctgatc cagttttttc tttaattatt ggtaggggag aataatggat tagaataaat
     2521 ctacctataa gtagtagtaa aaaaaagagc actatgataa tatctttctt aagagtaacc
     2581 gctttaataa aaaatccaga atattctaac ataaatggag tattcttaga atcaaaagga
     2641 gaaagaagat ggtttatact tatacggagc aagaaaagat gaaagtttta gacacattca
     2701 tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa aaagaaatac atcctattga
     2761 aagaagtctt gaaacgcttt gaccatggag tgacttatac agaaacagag gttaattcga
     2821 tactgctgaa cgtattttct agtggcgatt atgtcgaaca aagacgttat ctaatcacat
     2881 ttggcttttt caaaagaagt tcagacggtc gggcatatca gatgatggga attgaaaatt
     2941 agaagtttta atttatactc attgaaaaaa gttcaagtat gcgatattgg ttctacaccg
     3001 catacttgaa ctttttttgt catctgtatc tataatacaa agccaagaac gttattcaaa
     3061 atatggatag ctatgataaa agctaatgag cgattcgcag ttaaataagc aatcgtcagt
     3121 aaaatcgttc caggagcgta catgaaaaaa tcaattggat gcgttacatg aataaagata
     3181 attctctttt atttgtacga ataaatacca aaacgtacct cctaaaatta gattttggac
     3241 agatgccctt taaaaagata acaagatttc gttcttgaaa caatatatat gcttttattc
     3301 caatgaggtt gtgaaaacag tattttgtcc tgagcgttag aaaattgaaa atggggaata
     3361 gcaactcgta ctttaccatt ttttaagtga atacaagatc agctagttga acaccgttta
     3421 ttcgtgatga gggacacaac actgcagtcg acacatttct cttttatgtt caaagataac
     3481 aaatatttaa aacaaagaaa aatagtagat aatcccgtcc taactctttt attcttccc
//
LOCUS       DASJON010000231         3528 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJON010000231 DASJON010000000
VERSION     DASJON010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.236"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="U3989_002645"
     CDS             complement(<1..2232)
                     /locus_tag="U3989_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716060.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="U3989_002646"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="U3989_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          997 a          778 c          551 g         1202 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaa
//
LOCUS       DASJON010000232         3528 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJON010000232 DASJON010000000
VERSION     DASJON010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.237"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            89..562
                     /locus_tag="U3989_002647"
     CDS             89..562
                     /locus_tag="U3989_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEN1716061.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            636..1076
                     /locus_tag="U3989_002648"
     CDS             636..1076
                     /locus_tag="U3989_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN1716062.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            1089..1298
                     /gene="copZ"
                     /locus_tag="U3989_002649"
     CDS             1089..1298
                     /gene="copZ"
                     /locus_tag="U3989_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN1716063.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            1298..3484
                     /locus_tag="U3989_002650"
     CDS             1298..3484
                     /locus_tag="U3989_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN1716064.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
BASE COUNT         1213 a          603 c          813 g          899 t
ORIGIN      
        1 tttttttgcg gcgttataag tggtatgatc gaaccaaatt ccaaagagaa gtgagggaga
       61 agaatgaaga tcatcgtgat tggacaaagt gggtcgggaa aatcgtccct cgcaaggaag
      121 atcaaagaaa tcaccggctt tccgcattca ggcaaaaaag tggtttttgc aagaacagca
      181 atcgtttatg gtatctaatc cgtcatggat cgttgaagga aattatactt ctacgttaga
      241 ggaaaggatt aaagaagcag ataagatcat ttggcttaaa gtgccgcgtt atctcgccat
      301 ctaccgagta gtttggcgtt ctctaaaaag aaagataaac aaaaaatctc ggccagatat
      361 gcctgaatca ttttcggaga aactaaacag gaaatactta gaatttttat cttttatctg
      421 gcattttgaa ggaaacaatg aaccaaaaat acagcagctg atccaagtag ctaatgcaag
      481 aaataaactg atcatcctta aaacaagacg agagaaacaa gaatttttaa ctaaattgaa
      541 aatagagagc aaaaataatt gacagttttt gtttacaagt gtaatctgta ttcataagtt
      601 aatgattaca tttgtaaacg aaaggtgtga aataaatgga aaaagtcaaa acgccagtaa
      661 agattagtga ttcagagtgg gagatcatgc gtgttatttg gacacttgga cagacgacag
      721 cccaagaaat cacgcagatc ttagctgatt cgatggattg gaaaccagca acgatcaaaa
      781 ctttgctggg tcgtttggta aaaaaagaag tgatctggac agaacaagaa ggaaaaaaat
      841 tcatttatca tccagctgtc tcggagatgg aaacagttcg cagtgcgacg gaaaatcttt
      901 tttcccatat ctgtgcaaaa aggattggag agacgattgc ggatctagta gaagaagcca
      961 ctttgactca agaagatatc aatgagattt tgaaacaact ggaacaaaag caaccagtag
     1021 atacgatcga atgtaactgt atcccaggtc aatgtgaatg taaacaacat caataggagg
     1081 aagaaaaaat gaaacaacaa ttttcaatcg aaggtatgag ctgcaatcat tgtgcagcaa
     1141 gagtagaaga aacagtaagc gcacttgacg gcgtgcaaaa agtgaaagta aatttgaaaa
     1201 aagcaaacgg tacggtcaag tttgatgaaa caaaagttca atccgaaaag atttgtcaag
     1261 caatcaatgg attgggttat aaagcagagg tgatttgatg gcaacagcag aaaccaaaat
     1321 ggatacgttc gtcattacag gtatgacttg tgcaaattgt tctgctagga tcgaaaaaga
     1381 gctgaacgga caacctggtg tggtcaatgc cacagtcaac ttggccactg aaaaagcaag
     1441 tgtcaaatat gaagggacga ccactgaaaa attaatccaa agcgtagaaa acatcggcta
     1501 tggtgcaatt ttgtacgatg aagcccacaa acaaaaaatt gcagaagaaa agcaagcgta
     1561 tttgaaaaaa atgctattcg atcttatatt gagtacggtt ttgactttgc cgttgatgtt
     1621 gtcaatgatt gctatgatgt taggaagcca tgcggcgatt gttcactttt ttcattttcc
     1681 aatcgtccag ttagtgctgt ctgctcctgt ccaattttat gtcggtgctc gtttttataa
     1741 aggtgcgtac catgcaatca agtcgaaagc accaaatatg gatgtattgg tagcaattgg
     1801 aacatcggct gcctttgcat tgagcattta caacggattt ttccgtggac acccccaaga
     1861 tctttatttt gaaagcagca gtatgatcat cacgctgatt ttgctcggca aatatttgga
     1921 acatacagca aaaacgaaaa caggtaatgc aatcaaacaa ttgatgtctc ttcaaacaaa
     1981 aaccgcccaa gtgataagaa acgggaaaga agaaacgtta gcgattgaag aagtagtcgt
     2041 tggtgatcaa ctagtcatcc gtcctggtga gcagattcca gcagatggtc gaattatttc
     2101 aggaagcagt gcgatagatg aaagtatgct tactggtgaa aatcttcctg tagaaaagaa
     2161 tccggatgac acgctttttg gcgggacaat caatacgaat ggtctgctac acatggaagt
     2221 cacacaagtc ggcaagcaga cagtattagc gcaaatcatt caaatggtgg aagatgctca
     2281 agggagcaaa gccccaatcc aaaaaatcgc agatcggatc tcagggattt ttgttccgat
     2341 cgtattagtc atcgcgttta tcacgctcat agctacaggt ttgatcactg gtgattggca
     2401 actcgcattg atccatagtg tctctgtatt agtcattgcg tgtccatgtg cattaggttt
     2461 agcgactcca acagctatta tggtaggaac gggtgttggt gcacgaaatg gaattttaat
     2521 taaaggcgga gaagcattag aagcagctgc tcacttagac agcattgttt tggataaaac
     2581 tgggacgatt acagaaggaa agcccaaagt tacagatcta gtcggttcta aagaagtcct
     2641 atctattttt tatactttag aacaagcttc tgaacatcca ctaggtaaag caatcgtgga
     2701 atacggaaaa ctgcaagaag cagcaacata cgatatgatt gattttacag ctcacccagg
     2761 tgctggaatc agcggtacga ttaacggagt acgctatttt gcgggtacac gtaaacgatt
     2821 aattgaattg aacctatcat ttgatgaata ccaagaacac gcgttagaat tagaacagca
     2881 gggaaaaaca gtcatgtttt tagctgatga aaagcaagtg atcgggttga ttgccgtggc
     2941 tgatcaaatc aaactagaag ccaaacaagc aatcaagcag ctccagaata aaggattaga
     3001 tgtttttatg ctgacaggag acaacaaact ggcagccgaa acgattggta aacaagttgg
     3061 tattgatcca aagcatatct ttgctgaagt acttccagag gataaagcag cctacgtaga
     3121 aaaacttcag aaagatggca aaaaagtcgg tatggcaggc gatggaatca atgatgcacc
     3181 ggctttagca ttagctgatg ttggtatggc aatgggaagt ggaacggata tcgcgatgga
     3241 aacagccgat gtgacgctga tgagtagcag tttggcatcc attgcacaaa caatcgagtt
     3301 gtctagggtg acattgagaa aaatcaaaca aaatcttttc tgggcttttg tctataatac
     3361 gattggtatt ccttttgccg cactaggctt tttgaatcca atcattgcag gaggagcgat
     3421 ggcgtttagt tccgtcagtg tattgttgaa ctccctgagc ttaaatcggc acatgaaaaa
     3481 gtaagagaag ggatggagta aaaatgaatg ggaacgatat ggagaaaa
//
LOCUS       DASJON010000233         3378 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.238, whole genome shotgun sequence.
ACCESSION   DASJON010000233 DASJON010000000
VERSION     DASJON010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.238"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="U3989_002651"
     CDS             132..344
                     /locus_tag="U3989_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716065.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..721
                     /locus_tag="U3989_002652"
                     /pseudo
     CDS             506..721
                     /locus_tag="U3989_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            800..1926
                     /locus_tag="U3989_002653"
     CDS             join(800..1028,1028..1926)
                     /locus_tag="U3989_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1716066.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            1964..2275
                     /locus_tag="U3989_002654"
     CDS             1964..2275
                     /locus_tag="U3989_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN1716067.1"
                     /translation="MHTFSKNKLKDINSIDNYYIQFKGFDFNSSTEFKLALYAQFFIE
                     KLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLSYEMISKVLS
                     L"
     gene            2282..2794
                     /locus_tag="U3989_002655"
     CDS             2282..2794
                     /locus_tag="U3989_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716068.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(2898..>3378)
                     /locus_tag="U3989_002656"
     CDS             complement(2898..>3378)
                     /locus_tag="U3989_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1716069.1"
                     /translation="GQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTE
                     AMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNL
                     LTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT         1248 a          480 c          572 g         1078 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 tggtgttagt atcaaatctt agacagcttt tcgtagagac aagaataaat taaacttatc
      781 tacgagagga tgaattttta tgacccgata tgaagaaaat tttaaacaaa tgattgttga
      841 actgaatcaa actggacgtt ctgttcgagg gttagcgaaa gaatatggct tatctgaagc
      901 aacgatttac aaatggaaga atttatattt acctgatcag tccacaggac tgactggaaa
      961 agaagtagct gaactgagaa aagaaaatgc tcgtttaaat gaggaacttg aaatcttaaa
     1021 aaaagccgca gccatattct ctcggaaaac ctaaattcgc ttgttcaatt tattgaaaaa
     1081 tggtgtaagg attacactgt ttctttgtta tgtcggttat tagaaatccc tagaagtgtc
     1141 tattattttt ataagaataa accgttgaca gctacagaaa tcagaaataa taagttgaaa
     1201 aagaagattt caacaatttt ttttacgaat aaacaacgct atggtgccac aaagatccat
     1261 caagttttat taaaagaagg gatttcagta tctcttaaac atgtccaaaa gctaatgaaa
     1321 caattaaatt taaggtcgat tgtagttaaa aaatatagac ctcaaaggtc taataaaccg
     1381 atcatttcaa aagagaatct cttaaatcag gacttttcta ctgaaacgat ctgtgagaaa
     1441 tgggcagctg acattacgta cattcctact aagaaaaatg gttggtgtta cttatcttca
     1501 atcatggatt tacacacgaa aaaaattatt agttatacat tttcaaaacg aatgactgtg
     1561 gattgtgtca ttcaaacatt gaataaagca aaaatacatt atcacattcc agaaggaatg
     1621 attctacaca ctgacttggg cagccaatac acagcaagag aagtggaaca atggcttaaa
     1681 accaacaaaa taaggcattc ttatagtaga aaaggaacac cttatgataa tgctggaatc
     1741 gaatctttcc atgcctcatt gaaaaaagaa gaagtctaca cgactagcta ctcagatttt
     1801 gaagaagcaa atcgagcgct atttagctac attgaaggat tttataaccg aaatcgaatc
     1861 catagctcga ttcactatct aaccccacag gaatttgaag aattagcaaa agaaaaaatg
     1921 gcttaaccgt ctctactaaa atgtgtccaa gatattgact caaatccata cttttagtaa
     1981 aaataaatta aaagatataa attcaattga taattattat atacaattta aaggttttga
     2041 ctttaatagc tctaccgaat ttaagttagc actttatgca caatttttta ttgaaaaatt
     2101 aaataggttt caagaaatag ttgaagatag tgattttaac acttttaata gccataaacc
     2161 gatgagaggt acttatataa aatttctcga aaattataat gatttaaaat ctaccaataa
     2221 ttatcaattt ggaaacttgt cttatgagat gataagtaaa gttctaagtt tgtgatgata
     2281 aatgaaatta aatagtataa atgatttatt tgctattata ggtgttattg ctaacatact
     2341 tttttcgtgt ttgaatttta gatttactca aaagaacaaa aagaacaaac tcagtataga
     2401 acaaatgaaa aaagacattg aaactgattt aaaaaatgac cataaagaaa gaagaaaaat
     2461 tgaagaatat acttctttaa tggaaaagtg taaagcaact gatgcaattt ttagcttttt
     2521 aagaaaaagt gatgagataa tcacacgtga aaatatagat ttagaaaatg agattagtaa
     2581 acttgaatct atcttttccg atataaattc tcttaagtat ttaatcgtcg aagaaaattc
     2641 ggatagcttt ttagaatttg aaaaatatct aacagatctt atagataata gaaataatga
     2701 attaatgaga caaaacattg caagattcct tactaaatta gtttctgaat ggaaaagtat
     2761 tatttctcaa aatatggata aattaagata ttaaggatgt tagttttaaa ataaaggctc
     2821 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gagtcagaat gaaccacatt
     2881 ctggctcaga ttttttgtta tcttacattg attaattgat tgatcaaaaa gattctaatt
     2941 ctcatgttct caaatgattt aaatccgtag gatactcgtt tcattgtctt tatgtgggta
     3001 ttcttcgctt ctatttttcc attggaataa ggatagatca ttgcgttggt gatgccttct
     3061 tcataggtca gaaggttttg aagcttttcc cgaaagctgt catctaacgt ttcgggaagt
     3121 tctgccaata aggagaaaaa taagtcaggg tctttgtctc gaaaggcttc aactaattca
     3181 tgaaaaaagg gatacgcctc ctttaggggc ggagaaaact caagcaatcg atcaatcatc
     3241 attgcttcag taagaaatgg gtattttggt gctcggaaac ttttccatgt tttgtattca
     3301 taatggttga tgtttgcacg attttttagc aaaaagcgcc agttcttttt cagtttttct
     3361 gcctggcttt tctgtccg
//
LOCUS       DASJON010000234         3302 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJON010000234 DASJON010000000
VERSION     DASJON010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.239"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1170
                     /locus_tag="U3989_002657"
     CDS             <1..1170
                     /locus_tag="U3989_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1716070.1"
                     /translation="PIAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFS
                     QNGSQLRTSLLTIAKEWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKM
                     LAKEGFSNMSIFQTAKGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATV
                     DKADTMKVTTCLNPLHTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVES
                     PGIIDPKIFLEEVITVRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQ
                     TLVFIPLTLAAWLRYLIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKM
                     APILSNKEIFGVDLYQAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1161..2243
                     /gene="uxuA"
                     /locus_tag="U3989_002658"
     CDS             1161..2243
                     /gene="uxuA"
                     /locus_tag="U3989_002658"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1716071.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2351..>3302)
                     /locus_tag="U3989_002659"
     CDS             complement(2351..>3302)
                     /locus_tag="U3989_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEN1716072.1"
                     /translation="FKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYE
                     YIDFFIDLSVDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPH
                     VLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQE
                     HSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIG
                     VNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYIL
                     KGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         1068 a          570 c          650 g         1014 t
ORIGIN      
        1 ccgattgcag tcgatataaa aaatggtcca aagaagccat cacatacaat gagtattctt
       61 gctgtgctac tttataaacg ttttttatct ggagcttggc cgattgcact tgtctctaca
      121 gataattttt cacaaaacgg tagtcaatta cgtacttctt tgctaacgat cgcaaaagaa
      181 tggcaaaagg aaggttatgt acaaaataca tttatccaat acttatcgaa tgaagaaatt
      241 gttgcgtttc catggacgat gattgatcgt atcaccccga atccttcctt gtcagttagc
      301 aaaatgttgg caaaagaggg cttttcaaat atgtcgattt tccaaaccgc aaaaggaaat
      361 aatattgctc catttgtcaa tacagaggac atccattatt taatcatgga agatcatttt
      421 cccaatggac gtccgaaatt cgaaaaagct ggggtattct tgactgatcg tgcaacggta
      481 gataaagcag atacgatgaa ggtgacgact tgccttaatc ctttgcatac gtctttagct
      541 attatgggtt gtttactggg gtataggagc atagctgagg agatgaaaga tccagattta
      601 tcgctgctag tttctgaaat cgggtacaat gaaggcctcc cagttgtgga gtctccaggt
      661 atcattgatc caaagatctt tctggaagaa gtaattactg tgcgcttttc taatccaaat
      721 attcctaata tgcctcagcg aatcgccacg gatacctctc aaaaggtggc gattcgttat
      781 ggtgagacga ttaaaaaata tatggaaata gaagttaaag acccgcagac acttgtattc
      841 ataccattga ctctcgctgc ttggcttcga taccttattg gcattgatga tcaaggaaaa
      901 ccattcatcc cgagtccaga cccactattg acagaccttc agcataagct ttccgatatt
      961 caactgggta cgcaagatac atcacatatt catgagaaaa tggctcctat tttgtcaaat
     1021 aaagaaattt ttggtgtaga tctttaccaa gcagagcttg gcagaaaaat agaagaaatg
     1081 ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa cattacagga ttacttacaa
     1141 gaacatggag gaaatttatc atggaaatga cttttagatg gtacggatca caggaagaaa
     1201 agattacctt agatgaaatc agacagattc ctgcggtcaa agggattgta ggcacattga
     1261 tggatatacc agtaggagag gtatggccta gggagaggat caaagaacta aaagaagaaa
     1321 ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat gatgccatta
     1381 aaattggatt accttcccgt gagcagtata tcgaaaacta taagcagaca atccgaaact
     1441 tggcagaata tggaatagaa gtcatctgct ataattttat gcctgtattt gactgggtga
     1501 aatctgacct tgactaccgc ttagaagacg gctcatccac gttagctttt atatctgatg
     1561 atattccaga tgatccaaga gaaatcgtcg cgcgcatcga acattccagc aatgaatttg
     1621 gattgccggg atgggaacct gagcgcttag ctcctatcaa atccttgctt gaagcctatg
     1681 catcagtaga cgaagaaaaa cttcgagaaa attttgccta ttttctccaa tcaatcatcc
     1741 caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg ccatactcga
     1801 tttttggatt agctcgaatc gtaaagaatc gagaagattt agattggttg tgtcgcatag
     1861 tagacagccc ttataacgga atcactcttt gtacagggag tattgcagaa gatccagaaa
     1921 atgatgtata tgggatttta gcagaatttt gccaaagaga tcgaatccca tttgcacatg
     1981 ttcgaaatat taaattttta gctaatcgag atttctatga ggcaccacat aaatccgaat
     2041 acggttcatt agacatgtac cgcatactaa aaacaatgta tgacaacgga tttaacggat
     2101 acatccgtcc tgatcatgga agaatgatct ggaatgagac aggaagacca ggatacggct
     2161 tatatgatcg agcattaggt gctgtttatc taaatggatt atgggaagca atcgaaaaag
     2221 gaaacaaaag taaagataat tagtgaaaaa aactgtaatt ttattatgta aataagaagg
     2281 atgttcaaat tcttgataca taaatccgga ggttgttaat aagcattcgg atttatgtat
     2341 cttatttggt ctaggaagta acttgatttt cttttttcga ttgatccatg agtgcttctt
     2401 cttttaattc aaagatatat tctgagatcc tcataatcac ttttttccag tcttctggtg
     2461 tgatgattcc ttttaaaata tagatttttt tgatatgaga aggcaattgt ttcaaagggc
     2521 agtcgctgat aacgatgttg cagttctcca taaaatcatt ttgtattttg atcgattcat
     2581 ttgaaaaaat ttgttctatc ttaacgatca gatctttact gatataaaaa tcacgcgtat
     2641 agttcacacc aatcacaatt cgcggctgtt ttttgtagcg atcatacaaa tgtactaggt
     2701 cttcaaccat ccattgcaca ttttcctttt ctaaaataac tggtaacaag tgttgatttt
     2761 gttctctgaa atccatataa aattgttcaa tggcggtttg gacttctttc aatacgctat
     2821 gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag ttaagaaatc
     2881 cattataaag tttatttcgt gcgaatgcca atgagtaaag aatcttttct ttttcaggta
     2941 taaacaaatc aaatacagta caaagggact ccaccatttc tcttgaataa agaacatgag
     3001 gaacgtttgc tttttttaaa agtttgtatt gctccaagct tgtttcttcc gattcgattt
     3061 tcgtgataga aagtcgagct aaaatattca ctaagatgcg ttgttcgcga caaattggca
     3121 gattaggtcg caaaatatct acagagagat caataaaaaa gtcaatatat tcatactctg
     3181 tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta
     3241 atagaaaata taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa
     3301 aa
//
LOCUS       DASJON010000235         3280 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJON010000235 DASJON010000000
VERSION     DASJON010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.240"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..544)
                     /locus_tag="U3989_002660"
     CDS             complement(14..544)
                     /locus_tag="U3989_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1716073.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(643..993)
                     /locus_tag="U3989_002661"
     CDS             complement(643..993)
                     /locus_tag="U3989_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN1716074.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1150..2679)
                     /gene="ahpF"
                     /locus_tag="U3989_002662"
     CDS             complement(1150..2679)
                     /gene="ahpF"
                     /locus_tag="U3989_002662"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN1716075.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2689..3252)
                     /gene="ahpC"
                     /locus_tag="U3989_002663"
     CDS             complement(2689..3252)
                     /gene="ahpC"
                     /locus_tag="U3989_002663"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN1716076.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         1035 a          674 c          545 g         1026 t
ORIGIN      
        1 taactaaaaa tgattaacgt atagtcattt ttcttattct gatgaaattg ataatctcct
       61 ggatgcccat gaaaatcaaa ataaagccga atatttggaa tagaagcatc aaacttttga
      121 atggattgaa caataagacg aatccgccaa tcaacaacag aatactacta taaacactcc
      181 aacttttagc ttttgtattc atgctgaaga acaactggaa caagctgttg atgataacag
      241 ctaatccaag aagaatcggc aggatagaaa cgattgcagg tgcaaaagca tagacgatca
      301 gagctaagat caggaaaaaa agaccactga acagaccgat actccaagaa cctgttttcc
      361 ttttaaaacg gtaagcctca aaaagattca acaacccaag caaaacaaga taggcggtga
      421 tcagataacc gataaaatga aaaacagcag aagggtttag cacaatcgca atcccagaaa
      481 taatataaac agcagctctt aataaggcgt atttttgaat attttgttta atcgtctcaa
      541 acatataaga aaacctcctt tgacttattt taacgtgaac tagtcatttt accaaattaa
      601 atgttacaaa atgagcgaag gagaagaata tctctggaaa aatcaacttc tgaggatctt
      661 ttccattttt ttgccttttg ctagctcatc gatcatttta tctaaataac gaatttcttt
      721 catcaagtta tcctcgatgt cttctactcg tacaccacaa acaacacctg taatcaagga
      781 acgtgaagga ttggggacag gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg
      841 tagttgatat tctagttctg tttgggaata acccgtcagc caacaaatga tttcatctac
      901 ttcctctttt atacgatctt ttctctccgc ttttgctaca tacataggat aaacgcttgc
      961 aaaacttgtc gtaaaaatac gattttgcgc cataatcagt cacctttctt tttttcaaat
     1021 tatttactac ttatagtata taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa
     1081 aaaggattgt gacaaaattc ttgtcaccat ccttttttcc gctctctgac tttttacaat
     1141 cttcatagct caagtgcgca tcagataatc gaatgctcct aaagctgcag tagctcctga
     1201 acccattgaa acaatgattt gtttgtatgg actatctgta caatcaccag ctgcgaaaac
     1261 gccggggata ttcgttgcgc catttttctc ggtcactatt tcatttcttg gattcatttc
     1321 gatcgttcta tctagccaat ccgtattagg gagtaagccg actagaatga atactcctga
     1381 aacagctagt gtatgcatct ccatcgtacg gcgatcgata taggagagag attctacctg
     1441 attcgtaccg ctgatttctt tcgtcgctgc atgtttgata acagtgacat tcgcaagaga
     1501 gactagttta tcctgtaaga tttgatctgc ttttaattct ggtaagaatt ctagaacata
     1561 tacatgtttt gctaacccag ccaaatcgat tgctgcttca acaccagaat taccaccacc
     1621 gataacagca atctcttttc ctttaaataa agggccgtca cagtgtgggc aataagcaat
     1681 tcctttattt ttaaactctt tttcgcctgg tacattgata gaacgccagt gcgcacctgt
     1741 cgcgatgatg gcggtttttg ctttaagtga tataccgtta gctaagccaa tattaataaa
     1801 atgttgctct ttatttaagg aaacggcctg ttgattcatc ataatgtcga tagggtaact
     1861 acgcacatga ctttctactt gctgcatcaa ttttggacct tctgtgtaag aggtaccaat
     1921 catattttca atccctagtg tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc
     1981 tgtacggatt cctttacgtg ctgcatagat agctgcacta ctagctgctg gtccaccgcc
     2041 aatcactaat acatcataag gttctttttc aaacaactct tcttcaatca aagggccagt
     2101 gattttttca acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa
     2161 aaacacagtt ggcactgcca tgattttttt atcatcgatt tctttttgga acatcccacc
     2221 ttcaatcatc gtatgagtaa tatttggatt taatacagct aatatattca atgcctgtac
     2281 aacgtcgggg caattatgac aagtcaagct aacataactg tcaaaatgca atgtttgtgt
     2341 tatttttttg attttaagac tcaaagattc actgattttc ggtgcccgtc cactaatttg
     2401 taacatagcc agtaaaaaag aggaaaattc atgaccgagg gggataccag caaaaacgat
     2461 tccactttct ttgccaactg gattgataga aaaactaggt gtgcgcatcg aatctctttt
     2521 ttcccaagag attttaggag acatttctgc tatttccttc gtaaattcag ctagtttttg
     2581 actattttca ctattatcca aactaacaga aaaaacaata ggagattcaa tcagtgctaa
     2641 atattgttcg agattttgtt tagtttcttt gtctaacata tccaccacct aaatctttcc
     2701 aacaagatca aaacttggtt tcaatgtttc gccactttct ttccattttg ccggacaaac
     2761 ttcacctggg tgggtacgta catattgagc agcgcgaatc ttatcaatca gtgcactagc
     2821 gtcacgaccg attccatctg cattgatttc catagcttga acgattccgt ctggatcaat
     2881 aataaatgtt cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga
     2941 aatctgatga gacggatcac cgatcatcac gtattcgatt ttaccgatag catctgatgt
     3001 atcatgccat gctttgtgag taaagtgggt atcggtagag caagaaaaga cttcaacacc
     3061 taaagatttc aatgtactgt attgttcttg taagtcttct aattcagtag gacaaacaaa
     3121 agtaaaatca gctggataga agcaaagaat actccagttc cccataagat ctttttcact
     3181 gacttctaaa aattctcctt gatgataagc atgtgcatta aattcttcga tttttttccc
     3241 aatcaatgac atagtaaaac ctccaataat aattatttat
//
LOCUS       DASJON010000236         3266 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJON010000236 DASJON010000000
VERSION     DASJON010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.242"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..305
                     /locus_tag="U3989_002664"
     CDS             <1..305
                     /locus_tag="U3989_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1716077.1"
                     /translation="FLKTMGADTGKIYRIYKIPAGLSGFFSGLKVAATYCVSGAVVGE
                     WLSAQAGLGYYMIRVKNSYQIDKVFAAIICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            320..1312
                     /locus_tag="U3989_002665"
     CDS             320..1312
                     /locus_tag="U3989_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1716078.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            1324..2061
                     /locus_tag="U3989_002666"
     CDS             1324..2061
                     /locus_tag="U3989_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1716079.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            2051..2773
                     /locus_tag="U3989_002667"
     CDS             2051..2773
                     /locus_tag="U3989_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1716080.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(2857..>3266)
                     /locus_tag="U3989_002668"
     CDS             complement(2857..>3266)
                     /locus_tag="U3989_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1716081.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         1058 a          570 c          710 g          928 t
ORIGIN      
        1 tctttttgaa aacgatgggg gcagatacag ggaagattta tcgaatatat aaaataccag
       61 cgggtttatc cggcttcttt tccggtttaa aagtggctgc tacttattgt gtcagcggag
      121 cagtcgttgg agaatggcta agcgctcaag caggcttagg gtattacatg atacgcgtaa
      181 aaaacagcta ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt
      241 tattgctgaa tggcgcttgt tcattattga aaaaagggta ttatcgtatg ctttatattt
      301 tttaagagaa ggggatcaaa tgaaaaaatt atggctttta ttaccgctac tgttgctttt
      361 atccgcttgt ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa
      421 agtgacactt gttttggatt atgtaccgaa tactaatcat actggcatct acctagcaaa
      481 agaaaaaggc tattataaag aggctggttt aaatgtccaa atcatcgaac caggagataa
      541 ttcgacaagt attggtctag taggtgcgga caaagcacaa tttggtgtaa gttatcaaga
      601 agatgtcact tatgctcacg cagatggaca gaatatacca gtcaaagcaa ttgctacagt
      661 gatcaaacat aatacttctg gattcgccac cctatcagac agtaacatcc attcaccaaa
      721 agattttgaa ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa
      781 agctgtgatg gaaaaagacg gcggggactt ttcaaaactg acaatggtag gaagcaatgg
      841 agaagggcca gaaagcttag gaaaatcctc agatatccaa tggtactttg aaggttggga
      901 tatgatcaaa gcaaaagaag cgggtattga actaaactat atcccattga aagaattaga
      961 tgaacggtta gattactata ctccggtaat tattacgaat gatcaactga tcaaaagtga
     1021 tccagaattg gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg aagctattaa
     1081 ggatccaaat gacagtgcta aactgttgca aaaatatgca aaagaaaatg atcggacgtt
     1141 tttagaagaa tcacaagcat tcttgtcaaa aaattatacg gatgatcctg agaattgggg
     1201 attgatggaa gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa acggattgat
     1261 caagcaagat gtaccttcgc aagaactatt cactaatcag tttataaaat aggagtgata
     1321 gatatgtcaa tccaaatcag ccacttgaat gtagtcattg aaaacctgaa aatattagaa
     1381 gacatccatt tcactatccc taaagatacc tttacatcga ttgtcgcgcc aagcggagcc
     1441 ggtaagtcaa cattgctgaa aactttaaca ggagtccagc ctatgacttc tggaacgatc
     1501 aaagtagacg atcaaaaagt aactggtctc catacagcat ttagttatat gccgcaagaa
     1561 gatatgttac ttccttggct gagcgtctat caaaacgtta cgttatatca aaaaatcaat
     1621 catctaacaa tcaatgaaga acaagtaagg gattatctgg atatctttgg gttaaacggc
     1681 tatgaacatt ttttgccgga acaattatct ggcgggatga agcagcgaac ggccttgtta
     1741 cgaacaatca tgaatccttc ttcttacttg cttcttgatg aaccatttgg agcgttagat
     1801 gctatgacca gagggcagat gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg
     1861 actactttac ttgtgacgca tgatatcgaa gaagcaattt atttatcaga caggattctt
     1921 gtgctttccg cccgtccggc acatgtgatt gctgagattc aagtgcctga aaaacgacgg
     1981 tcacgtgaat ggctactgca gcaatcagag ttgaagcaaa cgatttatca gttattggca
     2041 ggtgaattag atgttaagtg atgctcattg tcatttagat gggagtcaga gtttagcact
     2101 ccttcagcag gaacacacga ttttgacgat catcaattgt gattcgccag aagaatggaa
     2161 ggaaaacaga cagcttgcag caagtaagac tcaagcctta agttacggta tccatccttg
     2221 gaaagcagat agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat
     2281 cggtgaaata gggttagata atatttggac gaatgtaccg atgactacac aaaaaaaagt
     2341 atttgagcga cagcttgctt ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa
     2401 aggatgcgaa aaagagatct taaattatat ccaagcttac cctaatcact atttgatcca
     2461 ttggtattct tctttggact atcaacaaga ctatattgat ttaggctgtt atttcagcat
     2521 tggtcttgat ttgaaaaaga atccagcagt ctggcaacta gctaaagcag tcccaatcga
     2581 ccacttactt attgaaacgg atggactaca tgcagcagag tgggctttag gggggatttt
     2641 ttcagaaaaa gattatgtct cactgcttga agaacagtta aagacaatcg ctgagatcaa
     2701 agagctatca gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac gtttcattag
     2761 attaggtaaa taaaaaagag atgtgacaca actagtgtcg cacctctttt tggctttctt
     2821 tgtataaaag taaactgttt gattaataaa tgaatcttag taccaaccgt ttgcttccca
     2881 gaatgctttc gccgcttccc aagaaccata acgtgaagta acatattgtt ctgctactct
     2941 ttcttggttt gcagcagagt agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc
     3001 gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg cgatccattc
     3061 ttttgcagag cttgtgcttg ctggagtagc agcagtttct gttacagcag gagcaggttg
     3121 tgtttcagca acaggcgctg cttgtttttc cgtttgaacc gtttcttgta caacaggttg
     3181 ttcagcttgg actggtgctt cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga
     3241 gccttctgtt gggattgtta attgtt
//
LOCUS       DASJON010000237         3265 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.243, whole genome shotgun sequence.
ACCESSION   DASJON010000237 DASJON010000000
VERSION     DASJON010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.243"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(85..1032)
                     /locus_tag="U3989_002669"
     CDS             complement(85..1032)
                     /locus_tag="U3989_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN1716082.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1037..1789)
                     /locus_tag="U3989_002670"
     CDS             complement(1037..1789)
                     /locus_tag="U3989_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1716083.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1790..2698)
                     /locus_tag="U3989_002671"
     CDS             complement(1790..2698)
                     /locus_tag="U3989_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEN1716084.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(2698..>3265)
                     /locus_tag="U3989_002672"
     CDS             complement(2698..>3265)
                     /locus_tag="U3989_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1716085.1"
                     /translation="ISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWI
                     IDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTII
                     GEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFT
                     LLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         1075 a          612 c          406 g         1172 t
ORIGIN      
        1 tatcccagat ttgattttac aaaactcatc agtccttatt gacaaagagc ctgaatttac
       61 cctctaatct tatcttagta tgttttaatt gatttcagtt ttcaaataat tagtcattcc
      121 acttaaaata ccatatttcg gttgatagcc aattgactgg atcttagtaa tatctgctag
      181 agagtcatgg atatcacccg aacgctcttc tttgtattca agtgtcaatt ctacaccgag
      241 aatttcattg attgcatgaa tcaattccaa aagtgtggtt gctttacccg ttccaacatt
      301 gaattgtttc cccagtgcag cttcctcatt cgcaactaaa agtaatgctt ggattacatc
      361 atctatataa acaaagtctc tagactggct tccatcacca aataatgtaa atgaagttgc
      421 ttctcctacg atttgtttct tatagcgatc tacgagaata gaaatcacac cactgtatgg
      481 agaattcgga ttttgatttg gaccatagac attgaaaaaa cgaactgcgc taccgggtac
      541 atcatataaa tgacaataat ctagcacata ttgttctgca gcgaatttat caaccgcata
      601 aggcgtcaat gggcgaatca cggactcttc tttttttggt aaagtcggtt catccccata
      661 aacagccgct gaagaactaa acacgatccg ttttaaatca ggttgatact tgcggatcaa
      721 ttctaaaatc attaatacac tttcaaaatt cacacgatgt gtttctactg gtcgggcaac
      781 tgaatctgcc acactagcaa ctgctgctaa atgaaagata taatcaaatt tattagcttt
      841 catgatctct tccatcaagg atttctcagc gacatctccc tcaataaaag tgatattttt
      901 cgtcatatca agattttcta cttttcccat tgataagtca tcgacgacga ttaccacatg
      961 ttcttctcct aaatgattgg ctaaggtaga accaatgaag ccagccccac cggtgataag
     1021 ataacagctc atccttttac ctccgatata aataattata gatatcttga agctccgaag
     1081 aatcttctaa aaatttagga aacaaaaatt ctaaccattt aaaatcttct tcactatcaa
     1141 tatctaaaat tagataatct ctcattttta ttatcccaca ttgcccatca aaaatataat
     1201 tacttttttt tagaaattca ggtttaaaag aatacattga ggcattcatt tcaaaaactt
     1261 caggtgtctg ttgcctagat gtgtatcttg atttattaac tatagtaact tcattttcaa
     1321 ttctttttac catattaaaa tagggattac gtcttgcaga aacaactgaa aagtagacgt
     1381 ctttatctaa attttttaga tgttcatcta ttaattcttc aagattagaa acttcacgta
     1441 aaggtgaagt caaatctaaa tctataatta aatcaaatat attatcttct ctttctagtt
     1501 gtaagaatga atcacgtatc acttcaattt tccctacagt atctgtagct aattcaggtt
     1561 tacgttctac aattttcaca gacggattta acttagtcgt caattctact aactcattac
     1621 tgtccgtatt caaacaagtc gtgcaattat gttcaggatg tttttgttga tagatatcta
     1681 tcattgctaa agcgaaatgg actaggggct ttcctgccaa atatttcaaa tttttatttt
     1741 taaacccctt agacccagct ctaccacaaa tagtaaataa aatttccatt tatttctcct
     1801 cagctatttt caatacttcg tttgcatgat ataaatcatt ccagttttct ttgcctgtta
     1861 ctacgttttc aaagaaatat ttcaactcct tctcatacat atcattagca gattcaggca
     1921 tttctttaac aatgttttct tcaaaactta ttttattatt taataagtca acattgatac
     1981 attcatctaa agtaaacaat tgaaattttc ttgtaggaac tcttccaaaa taatctaaat
     2041 gaatttctat aattttatca tcgtatctca atatatatgt tgctaaatca ttactgttaa
     2101 tttcaagatt cgaatattta ccaaactgtt tttttactga tttagggaaa ccaaataggt
     2161 ttataatata atccaattca tgtatacagt ctaattctac tcctcccccc atagatttaa
     2221 aggcagaata attatttcta taatcagtac cttttctcca atcaggaaga taagtagaac
     2281 aaatagctct tacagaatat acttttttac tttgaatact attacgaaca acttccatga
     2341 tatttttata cctcagtgga gcagcaacat aaatattttt gtaatcataa ttatattgag
     2401 taagatcaat actctcagaa aaagcaggtt tttctaaaaa tatgtaatca actttttcaa
     2461 caattacatt tagtgtttcc agatgcttaa aagtcggatt tgtaataaaa gctatatcat
     2521 aatgtgtatc caaatcgtct ctattttgaa tatacgttat ttcttcaaac tctttttttc
     2581 cactgctact ataagctgtt atatcaaaat taatagaatt cttttttcca tagcttatta
     2641 gattttttag atgtctcttt ccaattgacc ctaacccaaa aaaaattact ttcatcatta
     2701 atttacctca atgtcgttaa ttaagttaat aatatactta tctttttcaa aagtatattg
     2761 gggttccatt ttcttttcca aaagcgtgaa aaattcttta atctcctcta aatatgcatc
     2821 ttcaataata gtatgtgaat aattattatc atgcataacc tcatcgtaca attgaatatc
     2881 tgtcatacta gaagtttctt cattccatga aaatagagaa tccgggttac cttcccattc
     2941 tattaaaatc ttttcaccaa ttatagttaa atttctttta gctactcttg aaacaacatc
     3001 tacacttaaa gttccaacta taccagtttc atgctcaata ataagagtga aactgtccga
     3061 ataatcaata tcaagattgg ataatttctg ggtcattaca gtaaacgatt taactttccc
     3121 aaatgtatct ataatccaag gtaattcaat agcgaatatt tctctacacc cattagtttt
     3181 tttgtttgaa acaaaaaaat tgcgataatc ttcccaagga tgccagtcgg gtaaatactg
     3241 tcctacatgg tattgataag aaatt
//
LOCUS       DASJON010000238         3222 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJON010000238 DASJON010000000
VERSION     DASJON010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.244"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..322
                     /locus_tag="U3989_002673"
     CDS             5..322
                     /locus_tag="U3989_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716086.1"
                     /translation="MGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHPSGSVNG
                     ASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKEKEDEHV
                     ATK"
     gene            306..2786
                     /locus_tag="U3989_002674"
     CDS             306..2786
                     /locus_tag="U3989_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716087.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            2842..>3222
                     /locus_tag="U3989_002675"
     CDS             2842..>3222
                     /locus_tag="U3989_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEN1716088.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQ"
BASE COUNT         1014 a          624 c          653 g          931 t
ORIGIN      
        1 cttattggga gggattattg caagtttgct gttcttcagc tggcaagagc cagtccaagc
       61 agataccaaa agttcaggta cgattacatt ttatgatcta agtccaattc cgcatccaag
      121 tggttcagtc aatggcgcat caaaacagca gacacagcta ccgatttata aagaatctaa
      181 tcaacatggc gtattcccgg agacgaatga acaaaagatc cgttttcttt atttgatcat
      241 tggtatagaa ttatggcttc ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga
      301 tgaacatgtt gctacaaaat aaattttcat tttttcgctc cttctttttt acttcgcttt
      361 ttgttttaag tatatttatc ttttcacctt cggcagaagc tgttggagca acgcctcctg
      421 ccatttctta tgatccatac acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg
      481 cagtcaatgg cgggaagctt gtccaactaa ctggggctaa ccaattgcct aaattgactg
      541 ctacttattt gaacgatggt tcaaatgatc cttggaaagg tggcacggtc cgttttcatt
      601 gggaaagcca tgtgaaacta acaaatgtac ttgatttggt gaatatcact gcacaaggat
      661 ttgcatttgt ggcgatcatc aatttgccgg aacatgtatc aagcagtgat gtattaaaag
      721 caattgactg gaacaacgcc tacttgtata ttgccggtgc ccccatcaag gttactagcg
      781 gaagtttatt atcagcagga agccatacct tgcgttttgg gatggggtcg tgggatacaa
      841 atactctatt gacagatatt attgctatta ttacgcgagg cgaattcaac gtggacgata
      901 tcccgcttca atttgatgtt acagctaatg tcgcgaagat gacagaaaac ggagataaat
      961 atgatacgag tccaaacaaa attttgacca agggtaaatt acaaccttct atgtctaaaa
     1021 aggccgtttt ttctgttgat ttttatgatt cgaataatat tatccaagat cgtatgttca
     1081 attcgatttt agctccagct gcaggtagat tttatcctaa attaactggc ggaaacttga
     1141 atccaggagc atatgcattt tctgcaagtg cagagataaa tacgtggaat agttacatct
     1201 caccaagtga ttcaacagga aaatataata catttgctga taccacagaa tacgtggatg
     1261 gaaataacca gaaccttcca ggctctacca atagtcggac attgacgatg gggctgactg
     1321 aaggacgttt tgatcagttg ccatttagtc gctttgatcg cgtagtcaac tattttacag
     1381 gaaaaaacgt tacacaagga gcaatactga ctcatactcc agcaagcaca agtggattga
     1441 atcagacagc caatatcgtg tacagtggga aagatgcgca aggcggattg ctttctcctg
     1501 ttgctttaaa ggtattggag cagtacaagc tagacggaac ggttttacca acaaacttaa
     1561 cagccgatag tcagtgggga aagctccttt cttctgcatc gtaccaagtc acggccaatt
     1621 ggtcagcaac tgaactaaaa aagggttcga tccattacag gctttattcg aaaggaaccc
     1681 aaaaattagc gacaggatgg acagatcaac ttttccaaac gatccaatca aataacgggg
     1741 gtgtaaatac agctacttct acactacctg cattggcttc agggcaatat tattttgatt
     1801 atcgcttggt ggacgatgta cttactaaca tgtatccgtc ttttgcgtac aaatggcagt
     1861 ctgaacaagc tggaatcacc actcttcctc aaataacagt ggctaatttt ccatctgtga
     1921 gtgcagatag tcagctaaaa aaccttagca gacaacctga gacaggagat cccctgtctg
     1981 ctctatcagg agatacgatt caagaaaatg tgacattcac gttaacaaaa tctggggatg
     2041 cgataaccga caaaaaaatc acgatctcac ttcctgaaaa tactacttat atcaatggtt
     2101 ccttaaaatt aaatggtaca actatcgcag ataccggcat tcaacaagga gtttctgtcc
     2161 cagcagatct attgagcaaa ataggagata ccattcatct tacttacaac tatcaattaa
     2221 atactgtaga tacttcttta caatccgtca gcatactgac aaaggcagct gtactttcca
     2281 gcaatattac attagcagat ggtgctaagc ttcctaaccc agttgtccaa acatctgcta
     2341 aaacaattct tgtccccaaa caagaattaa cattagttaa tgtaccagat gattttacat
     2401 ttggtaacga tctccccaaa ccgttgaaga caagttatta tgaatcaaaa ggcgattttt
     2461 cttttgatgt gcgggatact cggcttccaa gcacgtctcc ttggcaactg acaggtacat
     2521 tgacttcttt atttaagaat gatcaaggtc aagaattaag cggaacaaaa ttatacttca
     2581 atcattctgg atcaaagcaa ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg
     2641 atggaacagc taaaggagag gttctcgttg actttcccga taccgatggt ttactcttag
     2701 aggtaaatag cagtacgaat gctcaacctg gcgcaacgta tcaaggcatg gtcacatggg
     2761 aactgacagc tgggccaacc agctaagtga ttagaatttg tatggatctg tgataactgt
     2821 cacagatcct ctttttcaag gatgagaagg aaacttctgc tgttaaaatc agaaatgctt
     2881 gaaggagtgg acttggaact tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa
     2941 cttttccaac ttttattaga ggaaaaagga gaggtttccg ttaataacgt acttacccgc
     3001 ttatcagttg atcgggctac cttgaaagaa gatctgtttt atttacaagc taacttggaa
     3061 aatatcaaac aattggaaat tgttatccaa gaagggaata ttttattaat cagacatgga
     3121 aatatagcag cagtcgatgt ttattatcat tgttacttgc gctattctac aaaatatcag
     3181 atacttcttt atctgttgga aaatggctca tttgatcggc ag
//
LOCUS       DASJON010000239         3202 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJON010000239 DASJON010000000
VERSION     DASJON010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.245"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..293
                     /locus_tag="U3989_002676"
     CDS             <1..293
                     /locus_tag="U3989_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716089.1"
                     /translation="SYEPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGV
                     LIGTNSKVYQEVKEKIEAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(430..1149)
                     /locus_tag="U3989_002677"
     CDS             complement(430..1149)
                     /locus_tag="U3989_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1716090.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            1802..1897
                     /locus_tag="U3989_002678"
     CDS             1802..1897
                     /locus_tag="U3989_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1716091.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2200..2445)
                     /locus_tag="U3989_002679"
     CDS             complement(2200..2445)
                     /locus_tag="U3989_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716092.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            2790..3074
                     /locus_tag="U3989_002680"
     CDS             2790..3074
                     /locus_tag="U3989_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716093.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT         1065 a          597 c          500 g         1040 t
ORIGIN      
        1 tgagttatga acccgatacc aacacgacca ctaccagcaa tcaggcaaaa ctaaatgcta
       61 tgggaaaaga aatttccatc atgactgaaa tcatcgcaga atttgctgat ttatatctaa
      121 aagatacagg cgttcttatt ggcacaaatt ctaaagtata tcaagaagtg aaagaaaaaa
      181 tcgaagctaa actaaatcca tcaaatcgaa atcgaccaaa gaatcatcaa aaagaaacca
      241 gtcaaacagt cacttctaga aatttcgacc caacaaattt tctaaaacga taaaaaacag
      301 ataggagtta ttcctatctg ttttttattt agaagcccca tacaatatgc tagaatgtac
      361 tagcaatctt agtcggttgt aagggacttg tctacttgct taatgtaagg ggggcgagtt
      421 cttttttatt tattcttcta accagttacg cagagaaatc tttggcaaat ctgacgttag
      481 gcttgaaaaa gtaaattcta atttggcgat tttattgccc ttttttgctt ttatttttgt
      541 gactttaaga tcattgaagt atttttttag ttctcgtaat gcaggattta atactttttg
      601 atcaatgtta cccatttgat agtattctgg gatatccatt aagctcctaa actcttcaat
      661 acgaactaca taataaccag tagaacggta ctgcataagc agtcggaata gagtttttgc
      721 atacgtagaa cgcaacgacg tgaaagcaga taattcaaat ctactaaatt cagctgttaa
      781 accattaata atatattcta ggtccggatt aacactgact tcaacaaact tttttgtttt
      841 attgatttca aaaccagtaa acaaaacaaa gtagcgaata gaatcctcat cttcagtacg
      901 gtaagtaaga ttaagcatat ctttatagag cttttctaaa tcaccgacaa aggcttttgt
      961 agcagtcatc ttataatcac ttaactcttt taattcctcg aaagaaaatc taactttttt
     1021 caaaccacga tctttcatac gagcgcataa tgcaaaaaat aaatccatct gcttagcatt
     1081 gaaatttttc agcggtacta aattcaattc attttgatat ttgactgtta attccgtttt
     1141 ttcatccatt cctttcttcc ccccttacaa ataaataata acctttaaca accaactcgt
     1201 aaataggttg gttgttatta tacaaaagga tattagtact cagataaaaa gttgttagta
     1261 ctcagataaa aagttgttag tactcagata aaaagttgtt agtactcaga taaaaagttg
     1321 ttagtaccct tctgtatccg ttgggagagt aaggctcaac tgccccctaa aaagggttct
     1381 aaaaaatctt taaaaaataa ataaaataaa cgcgcgcgcg cgagcaaaat ttcccgccaa
     1441 aaaatcaccc actttttaaa ataaacaggt tcgcgcatga tattaaactg ccaatctagc
     1501 tccctatggt cgctgtaaga aggagtgatc gctacgctct cacattttac ttgcagcagt
     1561 cgcctttagg ctccttcgcc aaaaataatt cattagggac tcctttgtgg cgtcctgaga
     1621 gctcgtctaa gccgttctaa gacaagattc gataaaaact atgtccttcg ttctaaagcg
     1681 ccttacaggc ggtcagggca agccctaaca accccaaggc aataaaaaac ttaggcttat
     1741 ttttgctttt ttattgtata attgattgtc gcctatccga cagtcttgcg actggaagga
     1801 ggtgtgtttc gtgttcacta actttatttg ctcccttctt gttgggcttt taagcgcttt
     1861 gttcagttac tggttgaata accgaaacaa gaagtaaaac ttaattaagg cgactcagcc
     1921 cacaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtgttt cgtgttcact
     1981 tatttgcaat caaattataa cagaaataaa tatttatttc aaaaatatcc tacaaaagtc
     2041 taaaatccgt aattatttct tatatgcaac atttacaaat aaagtcgctc tgagagctcg
     2101 tatttttcga tttaagacgt tttatttagt ttttggtgta ttttcattgg tccaagctaa
     2161 aaaactctta gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa
     2221 ttcgctccat gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat
     2281 taatttttcc aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc
     2341 gtctgttaaa tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa
     2401 atattcgtaa ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat
     2461 aaaatcactt ctttgccgtc tatactctca aaaaaaatgt cgtttgaaaa actaaataaa
     2521 tttttgatcg tgcattttta aatttggtta tcgattacac gtgcagcact cccccccgaa
     2581 ttttaaagag cataaggaaa cgtgtaccca ctttctaaaa aaagtgggta gataggctgt
     2641 gcctatcacg tttctcagaa aaaaatttga tatcactttt tcgccatcgt gggtagctta
     2701 tgtatagcat tttggcaaaa aacagctata caaatatagt atttgtatgg tatacataaa
     2761 ttatacataa ttgcaaaagg agatttttta tggacatttt agttcggaat attgatccaa
     2821 agtttgttgc aacaatagac aaacgttgcc aagaactcac gaataaaaca ggagaaaaat
     2881 ggagtcgaaa taaatatcta aaagttttaa tcgaaagtga ttttgaccgt caacttacga
     2941 cttacaaaaa agaccaattc gatttattgc tcgataaatt tattgctatc caaacagaaa
     3001 atactcaaat attaagcgag tatgtctaca caaataatca aatcattgaa atgcttatcg
     3061 gaggaatgat ataaccatga gaaaaacttc tgtccaaaaa catgttactt tgtaccctag
     3121 tcatgttgac gttttagatt ccatcatcga aaaaaatcca gatatcaata actactcgga
     3181 agccattcgc tttttatgta tg
//
LOCUS       DASJON010000240         3198 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJON010000240 DASJON010000000
VERSION     DASJON010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.246"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..630)
                     /locus_tag="U3989_002681"
     CDS             complement(<1..630)
                     /locus_tag="U3989_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1716094.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIF"
     gene            complement(650..1129)
                     /locus_tag="U3989_002682"
     CDS             complement(650..1129)
                     /locus_tag="U3989_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HEN1716095.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1146..1607)
                     /locus_tag="U3989_002683"
     CDS             complement(1146..1607)
                     /locus_tag="U3989_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1716096.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(1621..2871)
                     /locus_tag="U3989_002684"
     CDS             complement(1621..2871)
                     /locus_tag="U3989_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEN1716097.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT         1004 a          657 c          432 g         1105 t
ORIGIN      
        1 aaaaataaac tttttttctg gatattgttc tgcaatgttt ttaactgctt ctaccaaatc
       61 gtgtacacct tttcgcttta tcaaaactcc cataaacaga aatcggcaca tttttgaatt
      121 ccactttatg gtatattctg gaactacaat agaattttct acaacaacag ttttacaatt
      181 tggctcaatt tctttaatta tcttcttcca tttttctcca agtacaatcg tggaatcaga
      241 ctctcttaac agcaagcgaa tctgattttg tttttttaca gaaacactat cataccattt
      301 tttaaattcc gatccatgaa gatggatgat tacaggaata tttaataatc taaaccactt
      361 attcagcaca tatttcctac taaaactccc tctgtacgac atgtgcatat gaataatatt
      421 aggtttactc aataaagaga tacctataat tttagcaaat gcatacataa aatataaaga
      481 tttaaatatt gcatttccac ccttataact cggtataaaa gtcatctcga tattcttttt
      541 ttcccaatta aaattgagca tttgtgtaat tatacttgta attccacctt ttaccgagct
      601 gtcattacca atcataagta ctctttgcat aacgctttac tccttatatt tatatttttt
      661 ctattaatac ctctatcgaa ttaataatcg tttccgtgtt agatacatac ttgttaaaca
      721 tccgaactct tgtttcttta acggcatctt ctaaattttc taattcataa caaggataga
      781 taatattcat ctcatcaaat tgtctaatta actgcagttg gtgatcatcg acatgctcac
      841 catacttagc caatcgagga acagcaatga cccttttacc tttttttacg gccccaatta
      901 tagcacctgt acctccatga gtaatcacca tttctgcttt ttgcatagtt tcagtaaact
      961 catccctatt tagaaatgct ttataggaat aatttagtgg tttataatca ctataaccac
     1021 tctgtgcaaa tgtactttcg attattactc ctttttcaat taaatcatca atagctttta
     1081 acaatctatc aaattgaaat ttttgtgaac ctaatgttat aaatatcatt tttttctcct
     1141 attatttaat atatcccacc caaataaata gcattaggat aaaattgtag catagtttcc
     1201 cactgaacat aaaattgatc tgcaaaatga tacatcaatt ttcctgtctg agtagggctg
     1261 gttacttttg caaacgattc aatataaatc agtttttttc ccatcatttt tgttattaag
     1321 cagataggga tcatagctaa aacacctgta caaatcacga gatccggttt ttcctttata
     1381 tatataataa ttgatttgaa taaattaata agtatagtac acaaaaaaaa cttttctttt
     1441 ctatttactt gtctcatgta gtacgttttt tcgccttttg actcagctga atattctgtc
     1501 tgttctgtta taacaaaact ctcatacttc tccatcagtg gttttaacat cattaactgt
     1561 tcataatgcc ccccagagga agccgcaaaa catatctttt tgttcatatt aacctctcta
     1621 tcagtcacgc tgaaaaatat ctctagtgta aactttttct ttaatatcat ccaagcaggt
     1681 atcatatcga ttggcaataa tagaatcaca catcgcctta aactgctcta aatcgttgat
     1741 aaccttactt ccgaaaaaag tctctccatc tttcagtgca ggctcataaa tcaccacagt
     1801 cgcaccttta gcttttacgc gcttcataac tccctggatt gaactctgac gaaaattatc
     1861 actgttgctc ttcatggtca gccggtatat accgattaaa actggatttt ccctgttgac
     1921 atcatactga ttatattcag cgtagttgta atagcccgcc atctgaagaa cacgatctgc
     1981 gatgaaatcc ttacgggtac gattagattc aacgatagcc tcaatcaaat ttccaggaac
     2041 atctgcatag tttgccaaca actgcttggt atcttttggc aagcaataac ctccatatcc
     2101 aaaggatgga ttgttgtagt gagttccaat acgaggatcg agacaaacac cattgattat
     2161 ctgctgagta ttcagcccct tcatttcagc ataagtgtct aattcattga aatagctgac
     2221 tcgcagtgct aaataggtat ttgcaaacag ctttactgct tcggcttcag tgaatcccat
     2281 aaaaagagta tccacgttct ccttaattgc acttttctgc agaagtgttg caaaaacatg
     2341 agcagcattt aacaagcgct catcagtcat atcagtacca acaataatac gagatggata
     2401 cagattatca tataaagcct tgctctcgcg cagaaactct ggactaaaaa taatgttctt
     2461 actccccgtt gtctcacgga ctgattcagt gtagccaaca ggaattgtag acttgatgac
     2521 catgatggta tcaggattca cgtccatcac aacctcaata actgactcaa cagcagaagt
     2581 atcgaaatgc tgagtacgac tgtcatagtt agtcggtgta gcaataacaa cgaaatctgc
     2641 ggatttataa gcgctccttg catccaaagt agcagttaag tcaagttctt tttctgtaag
     2701 atacttttca atatattcat cctgaattgg agacttccgt ttattaatca gttcaatctt
     2761 ttcagggata atgtctaccg cggttacctg gtgatgctgc gccaatagtg tggcgatact
     2821 caaaccaaca taaccggtgc cggctactgc gatgttgtac tttttcttca caatatctcc
     2881 tctttcctct cttaagtatg gagaatgtta ctcctacggc tattcatcat gtcaaaataa
     2941 cacagcttag aatgttgata aaataatttt ccagaatgtg ataaaatctc tttcatttat
     3001 aaaccacaca tctaatttta tctattctcc acaagacatg tagagtattc tttaataaca
     3061 agatacgacc ttactaaata cttcaaaaaa ttatcatatt taattcataa atataaatct
     3121 tcattttaaa tgtatgccca aaaatttatc tgtaatgcgt catcaaaatc tagattccaa
     3181 atctttatca atttttat
//
LOCUS       DASJON010000241         3121 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJON010000241 DASJON010000000
VERSION     DASJON010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.247"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1315
                     /locus_tag="U3989_002685"
     CDS             <1..1315
                     /locus_tag="U3989_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1716098.1"
                     /translation="GKGENIWDHWYELEPEKFFDQVGPEKTSQVYTKYKEDVQLMKET
                     GHNTFRTSIQWSHLIPEGTGEINQKAVSFYRDYFTELLDNGIEPFVNLYHFDMPLPLQ
                     EKGGWLSRETVDAYVSYAAACFELFGDLVKKWFTHNEPIVPVEGGYLYQFHYPNHINM
                     KEAVQVGYHENLASAKVIKAYHEGGHDGEIGIILNLTPSYPRDETNPEDVKAAKIADA
                     FFNRSFLDPAVKGEFPQELIEIVKELAIMPEIHEGDLELIRENTIDLLGINYYQPRRI
                     KAKETPINHEHSPMPDDYFDNYDMPNKKMNPYRGWEIYEKGIYDILTNTRENYGNIKC
                     YISENGMGVEGEERFVNSEGIIEDDYRIEFVTDHLKYVHQAIQEGTNCVGYHMWTCMD
                     NWSWTNAYKNRYGFIAVDLAQDGKRTIKKSGRWFKTVSEKNGFDA"
     gene            1396..1836
                     /locus_tag="U3989_002686"
     CDS             1396..1836
                     /locus_tag="U3989_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1716099.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            1984..2310
                     /locus_tag="U3989_002687"
     CDS             1984..2310
                     /locus_tag="U3989_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1716100.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            2311..3060
                     /locus_tag="U3989_002688"
     CDS             2311..3060
                     /locus_tag="U3989_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716101.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         1058 a          518 c          656 g          889 t
ORIGIN      
        1 tggtaaagga gaaaatattt gggatcattg gtatgaatta gagcctgaaa aattcttcga
       61 tcaagtagga cctgaaaaaa cttcgcaagt ttacacaaaa tacaaagaag atgttcagtt
      121 aatgaaagaa actggccata acacatttcg tacatctatc caatggagtc atttgattcc
      181 agaagggaca ggtgaaatca atcaaaaagc tgtttctttt tatcgagatt attttactga
      241 attgctggat aatggtattg aaccttttgt gaacctttac catttcgata tgccactacc
      301 actgcaagaa aaaggcggat ggttatcaag agaaacggtc gacgcttatg tttcttatgc
      361 agcagcgtgc tttgaactgt ttggagatct ggtcaaaaaa tggtttactc ataatgaacc
      421 aatcgtacct gtagaaggag gctacttgta tcaattccac tatccaaatc atatcaatat
      481 gaaagaagcg gtccaagtag gctatcatga gaatctagca agcgcaaagg taatcaaagc
      541 ctaccatgaa ggcggacatg atggtgagat cggaatcatc ttgaatctga caccaagcta
      601 tccgagagat gaaacgaacc cagaagatgt aaaagcggcg aagatcgctg acgcattctt
      661 caaccgatca ttccttgacc ctgcagtgaa aggagagttt ccacaagagt tgatcgaaat
      721 tgtcaaagaa ttggctataa tgccagagat ccatgaagga gacctagaac tcatccgtga
      781 aaacacgatt gacctgctag gaatcaatta ctatcagcct cgtcgaatca aagcaaaaga
      841 aactccaatc aatcacgaac atagccccat gccagatgat tattttgata attacgacat
      901 gcccaataaa aaaatgaatc catatcgtgg atgggaaatt tacgaaaaag gaatctatga
      961 tattttaacg aatacaagag aaaactatgg taatatcaaa tgctatattt ctgaaaatgg
     1021 gatgggggtc gaaggagaag aacgatttgt taacagcgaa ggaattattg aagatgatta
     1081 ccggatcgag tttgttacgg atcatttgaa atacgtccat caagcaatcc aagaaggaac
     1141 caattgtgtg ggttaccaca tgtggacatg tatggacaac tggtcttgga caaatgctta
     1201 taagaatcgc tatggcttta tcgctgttga tttggcacaa gacggtaaac ggacgatcaa
     1261 aaaatcaggt cgctggttta aaacggtctc tgaaaaaaac ggatttgatg cctgatattt
     1321 tttgataaaa cgttcacaaa atgtttgttc taacactgta atcgtgctac actaacagtg
     1381 aaggaggcgt ttacaatggt agatacttat cgtaatattt tagtagcaat tgatggttct
     1441 gaaaaggcag aaaaagcatt tcttgaggca atcacgattg ccaaacgaaa tcaggcaacg
     1501 ttgcacatcc tgtatgtcaa tgaagtaaca ggcaattact ttggtgattt tgcatttgtt
     1561 acaacaagcc ttcaggaaga attggatgag gttgcggaaa accagatgaa agaacatcga
     1621 aacttggcaa ttgaaaaagg cctgacagat atcgagacat atgttcttta cggttatcca
     1681 aagacactga ttgctaattt ccatgaatcg aaagaaccaa tcgatctgat tgtcatgggg
     1741 gcgacaggtc tgaatgcggt agaacgtgca ctagtaggtt ccactacttc ctatgtagtc
     1801 aaccacgcat catgtaacgt catggttgta aaataaataa attaagggaa cagaaagcaa
     1861 aaaagcataa cgcttctttt gctttctgtt ccttttattt ttttgtaaaa aattacttta
     1921 cctgatgaag taatcatgct attctaatta caacgacaga tggagtaata tggaggaaag
     1981 aaaatgattt caagtgatgg aatacgcgga tacaatgatg cgattatttt atcgatcctt
     2041 aacaaaaacg attcttatgg atatgaaatt tctaaaacga ttcgtgagag gacaaatgat
     2101 tcctatgtga tcaaagagac aacactttac tccgtttttg cccgtttaga aaagaatcaa
     2161 ctgattgttt cgtatcctgg agaagtgact catggaaaaa agcggacata ttataaaatc
     2221 actgaaatag ggcgtaatct tttagaagaa aaaaagcaag agtggctgac gacgaaagac
     2281 gtagtcgatc aatttttgga gggaaattaa atggatacaa tccgtaatta tttagacagt
     2341 ctttttatcg gagtacctca aagtaccgag atagataagc tgaaaactga tttgttggca
     2401 aatatggaag accattatca tgaattgatg ggagaaggaa aaaatgaaca ggaggccatc
     2461 ggtacagtaa tttcgacatt tggttcaatc gatgaattat tagaagaact agatgtcgaa
     2521 aaaaaacatc aagctgacga gacggaaaca aacacagctt ctatttattt aagcgaagca
     2581 gaaaactact ggaaagaata cagagcggca agtctccaag tggcatcagg tgttctattc
     2641 attagcttat cttttgcaag ttttcttttc ttttgttcag caggatatgt gttcatgggt
     2701 atttcttgtc tgattttcgg tatagcatta gctgtcggtt tttttattgc ttctggtatg
     2761 aaaatcacac gtttaaatca ctttttgcac catcggaaaa tacctgaaaa agtattagca
     2821 gaagcaaaag aaaaagaaga agagtatcag cgaagttttg gcttctcact gattgcagga
     2881 attggattat gtattttttc acttttccca cttttggctt ctttaatgtg gtatatggat
     2941 ggtagtatcg gtgcctctat attttttgtt acggtaggaa cgggtgtctt cttgatcatt
     3001 tatggcagtc tagtccgtca ttcttatcgt caattcactc aaagcgctta ttactggtga
     3061 atgtattcag atttgtaaaa atattctatt tatccggcgt agaaaaaaag actgtgacac
     3121 g
//
LOCUS       DASJON010000242         3104 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJON010000242 DASJON010000000
VERSION     DASJON010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.248"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..690)
                     /locus_tag="U3989_002689"
     CDS             complement(<1..690)
                     /locus_tag="U3989_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716102.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEI"
     gene            957..2657
                     /locus_tag="U3989_002690"
     CDS             957..2657
                     /locus_tag="U3989_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN1716103.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2627..2845
                     /locus_tag="U3989_002691"
     CDS             2627..2845
                     /locus_tag="U3989_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN1716104.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2865..>3104
                     /locus_tag="U3989_002692"
     CDS             2865..>3104
                     /locus_tag="U3989_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN1716105.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVIN"
BASE COUNT         1071 a          536 c          580 g          917 t
ORIGIN      
        1 gatttcctct tctcgatacc gggtgccttt taatttccaa atataatctt caatcacata
       61 gattgggata cgatatttac catcgatcaa accaacttga tcaaaagaaa aaatgatccc
      121 ttcctgcatc aaattacgca tttctttcaa taattccaca ctgatttttt caacaggtgt
      181 ttcttttttg aaaaaagatt tttttacatt gatcttatct gacaagatcg aacttaattt
      241 actagcaaaa atcctataaa attcatctaa atagcgataa taaatccgat tcagctgatc
      301 attaagatcg aagtatacaa aattattctg tagtttataa aaaaacggcg aatgaagatg
      361 tgttttcata tgaccaaaat ataataattc tgaaatttct aaaggtgtca attctttcag
      421 catcgttaaa tcagtaaagt cgatccactt caattcatcg aatccacgcc tgttacgaga
      481 agaggaaaag aaacgctgaa tattttctgt accacggatt actttcagcc ctgtatggga
      541 ttcatattct ccagcagaag cagatggatc aagcaagagc aagttttgag gaaaatgcac
      601 gatactgttt gcaaagtcag tagcagtcag cccttttgtt aaaacagcat tacttgtggt
      661 atcaatatgt acataaactg actcagtcat catgttcctc ctcttatttc ctcttaatct
      721 cttttatttt acaacatttg cattaaaaaa gcttgtttct tttattttaa aattccatta
      781 caaaatcacg ttttcattga aaacactctc attttcaatt aatttttgtg atttttgtat
      841 ttttttattt aaaggaggtt ttatgaattg ttttcttatg gaatctacaa agtcgaatac
      901 gagactcatg acctttttat gacgtactgg tatacttgaa gaaaaaggag agcgcgatgg
      961 agataaaaaa agtttacttt tcacagaaaa tgtcgctgta ttcgcctatc cagcaagcaa
     1021 aacaacccct tccatttttt caaagtcatc aagaaagtag agacgctttt tggcgttctg
     1081 caaaaaagca atgtgctttt aaagcagtat cgaaaaaata taccgtctat tttactattg
     1141 ctaaaagtag aggagatgtt atttttaata atctgttgat tgaaggtgaa acttttgaat
     1201 ggaaaaaatt ttttcggtat atggaagctt gcgctcgttt ttttattaaa gaaaactgtt
     1261 gttttttatt tcaatctcct ctatcagaag aatggttgcg tatttttcat aaaaacggct
     1321 atcaaggaac cacacaatta aataaaaagc tggtctatca tacagcactt gttttaggtg
     1381 gaggaggagc gcacggtgct tatcagattg gtgtgtggca ggcgctgaag gaacatgata
     1441 tcaactttga gattatcaca ggtacctcgg taggtgcgtt gaacggcgca ttgattttac
     1501 aaggagatat gaaaaaagct gtgaatttat ggaaaaagct gtcgacaaga caagtgcttg
     1561 cgttaccaga aatggctgca gttgaagacc ttcgtgaacg cttctatcga gaaagacgcc
     1621 aaatgacgaa aacagcacta attgaaggcg gggtttcttc tgctccattg gaaaagatgg
     1681 tcaaagacaa tttggatttg tccttactca aacataatcc caaaatacgt ttatatacgg
     1741 tgtctactaa actgccagaa ctagcggaag tagtgactga tatccaacag acaaaaacag
     1801 aagaaatacc agattggata ctcgcctctg cttcatttta tcccgctatg gcttatcgaa
     1861 agatcggaaa aaataaatat gcagacggtg ggtatcgcaa caaaatacct attgatattg
     1921 cgattaacaa aggagcaaca gaggcatttg tcgtagatgt gcaagggcca ggcccagcga
     1981 aaaaaattcg tatgccagac atttttatcc attggaaatg tcaaacattg tggacactag
     2041 gcagctttct gctttttgat agccaaagga accaattaaa tttacaatta ggctatctgg
     2101 aaatgaaaaa acgattaggc tattactatg ggaactggta tacatttcat tctgttaaac
     2161 ctgcaagaac atactggcga ggatttttgt cttacttaac aaaagaaatt cagttagaat
     2221 tatctttttt caaaagcata aaattttggc aaaaactgcg taatttatat aagaacaggg
     2281 tcgtaccaga gacatgcgga ttggcgatgc tagaactttt agcaaaaaaa cattttttac
     2341 tgcccaatga aatctatcaa gtagacatga tgattcaaat gatctgccaa caagatataa
     2401 aaagtatgcc ggatgattta ttggcacaaa ttggtcaatt aagtactgaa gaatggcgaa
     2461 ggtatcgaaa ataccagcaa aaaatacaaa atgagcgcac gaaaactgtt tattttgatt
     2521 atctgctaca aggaaaacgc aaggatcgtt tgcagcatga attgcttaag caaccagttg
     2581 acacattact gatcctatat ttatattatc tcaaggagga acaaccatgg cacaagaatt
     2641 ttcatatgaa atcgtagaag aaatcgctat attgtcagaa aacaacaagg ggtggcgtaa
     2701 agaactgaac ctagtcagct ggaatggtcg cccgccaaaa ttcgatcttc gagattgggc
     2761 gccagaccat gaaaaaatgg ggaaaggcct gacattgacg aacgaagagt ttgaacaact
     2821 gcaaaaagca atagaaaata tgtaatgagg aaggaccctg tcgaatgaaa agtatgactg
     2881 gttttggcaa agccatccgc gaaacaaaag cgtatcagtt agatattgaa ataaaaagtg
     2941 tcaaccagcg ttttctagat gttcaaatac gaagtcctaa aatcctgaac tttgttgaaa
     3001 acgattttcg tcagcttatt aaacagcagt taagccgcgg tcgaatcgaa gtatttgtga
     3061 atctagctta tattggcaaa aacgaaaaac agatcgtcat caac
//
LOCUS       DASJON010000243         3096 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJON010000243 DASJON010000000
VERSION     DASJON010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.249"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..893)
                     /locus_tag="U3989_002693"
     CDS             complement(324..893)
                     /locus_tag="U3989_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1716106.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="U3989_002694"
     CDS             complement(967..2256)
                     /locus_tag="U3989_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716107.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..>3096
                     /locus_tag="U3989_002695"
     CDS             2514..>3096
                     /locus_tag="U3989_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1716108.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEK"
BASE COUNT          905 a          625 c          536 g         1030 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaag
//
LOCUS       DASJON010000244         3003 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.250, whole genome shotgun sequence.
ACCESSION   DASJON010000244 DASJON010000000
VERSION     DASJON010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.250"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="U3989_002696"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="U3989_002696"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN1716109.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="U3989_002697"
     CDS             complement(1032..1952)
                     /locus_tag="U3989_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1716110.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /gene="xth"
                     /locus_tag="U3989_002698"
     CDS             2065..2817
                     /gene="xth"
                     /locus_tag="U3989_002698"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN1716111.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          850 a          607 c          518 g         1028 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 cat
//
LOCUS       DASJON010000245         2992 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJON010000245 DASJON010000000
VERSION     DASJON010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.251"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(106..1053)
                     /locus_tag="U3989_002699"
     CDS             complement(106..1053)
                     /locus_tag="U3989_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1716112.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="U3989_002700"
     CDS             1299..1763
                     /locus_tag="U3989_002700"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN1716113.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="U3989_002701"
     CDS             complement(1825..2868)
                     /locus_tag="U3989_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN1716114.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          990 a          593 c          571 g          838 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DASJON010000246         2978 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJON010000246 DASJON010000000
VERSION     DASJON010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.252"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            385..543
                     /locus_tag="U3989_002702"
     CDS             385..543
                     /locus_tag="U3989_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1716115.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            615..1840
                     /locus_tag="U3989_002703"
                     /pseudo
     CDS             615..1840
                     /locus_tag="U3989_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1929..2321)
                     /gene="rpsI"
                     /locus_tag="U3989_002704"
     CDS             complement(1929..2321)
                     /gene="rpsI"
                     /locus_tag="U3989_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1716116.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2337..2780)
                     /gene="rplM"
                     /locus_tag="U3989_002705"
     CDS             complement(2337..2780)
                     /gene="rplM"
                     /locus_tag="U3989_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN1716117.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT          999 a          506 c          529 g          944 t
ORIGIN      
        1 accgtattat ctatattgaa ttctcggaat caagataaat ttttttccgt ttttgtttct
       61 tttttaatct tcttcttgtt attaatagtg attaggatga tattcctata ttgatctttg
      121 ataattagat ataatttttg aataacgttc cctgctattc gagctagaaa tacaagtacg
      181 ccatgaccta gttagcgaca actacttaga gatttctttt gttctgggac aacaaaatgc
      241 catgacaata gcagtaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt
      301 gaaattccta tgtgcttacc caaagcggtt caaaaatttt aaactattat tttaggagat
      361 gattttataa aatcgaaagc ctacttgaat aaaaaacaag cctgtgaatt tctaggaatt
      421 gataataata ctttagataa gtggattcgt gtatataatt ttccagtaat aaaaattgat
      481 ggcgtagttc gctttgacgt agaggatatc aaagatttca tgaatacatt caaaaaaaat
      541 tgaaattttc ttctcgtcac atatactata aatagcgaaa tgtactgcct tactttttaa
      601 aggagtgagg aagtttgcct acaaagcttt caaatgggaa atataaaact aatctgaggt
      661 atccaaaaaa attcagagaa atcactggaa ttgcatcaga gaagtatcaa aaagtatttc
      721 caactaggca attagctatt aaggccgaaa gggaaatgaa aaagaagatt gaaacagttt
      781 taagagagga gaatgcaaac tcactagaat tgaaaggaaa aataaaattt aaagattttt
      841 atgaatcaaa atggcttcct agatatgaac taggtcaaac tactaggtca aacagggctc
      901 caagtaatgt aacaattagt aatactaagg atatattccg attgcacatc cttccaatgt
      961 ttggtgaata tgcaatgaat tatctgaatt caaatactga gataatatca gatgaattaa
     1021 caaagaaatc gaaagaatac gcaaatataa aaattatcaa aggctatgtt cgctctatgt
     1081 ttgatattgc cgaaatacta tgttatatcg aattcaaccg aactaccaaa attattcaat
     1141 gtattaccgc gcctaagaaa atggctttag aagaaaaaag gataagggaa gggaatcaag
     1201 cattatcatc agaagagctg actgattgga tagaagcagt aaacaatgac ttaaataatc
     1261 acttgcttac acttcacgat tacacacttt tcatgcttac cttatatcta ggtgatagaa
     1321 aaagtgagac ttatgctctg caatggaaat atattgattt taataaaaat acggttcgtc
     1381 tcaaatatgc tttagataaa tatcaaagaa agacacatac aaaaggttgg aaagacaccg
     1441 ttattcaggc accagaagtt gtgatgacat tattaagaga atggaaatca gctcaggctg
     1501 aacaattgtt gaaactaaaa attatacaaa cacctgacca gtacctcttt acctattcca
     1561 aaccttctgg agaagtaaat tgtcctgttc acgctgacta cttaaactat cgaatcaatg
     1621 caataaaaaa aggcatcctg aattggctca attatctcca cacaaacttc gtcataccta
     1681 tgcaactata gctagggaag gtggagcaaa tatgaatcaa atctctaatg ctttaacgca
     1741 ctcggatatt tctactacaa aaatatacgt gaacacacct gacgtagttg ataaatctgt
     1801 ttttgaagca tttcaaaaag gattgaataa gtgtgattaa catgtgttct caaatgtgtt
     1861 cttattataa aaaaagacac tttccaaata attgaaaagt gtcctgaaat attgatataa
     1921 aaagaatatt aacgttttga gaattgtgaa gctttacgag cttttttaag acctggtttt
     1981 ttacgttcga ccatacgtgc gtcacgagta agtaagccag cacgtttaag agctaaacgg
     2041 aagtcaggat caacttctag caatgcacga gcgataccat gacggatagc tcctgattgt
     2101 ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat atgcgccttt tgtttcagta
     2161 acagcgaaag gttgattgat tacttcacgc aagtcagcat gtggaatgta ttcttcaaca
     2221 tcttttttat ttacagtaat tttgccagtt cctggtacta gacgtacgcg agcaactgcg
     2281 tttttacgac ggcctgtgcc gatatattga acttgtgcca atgaaattcc ctcctattag
     2341 attaagtttg tgatgtctaa aacttctggt tgttgtgctg catgtggatg ttctgctcca
     2401 ccgtatacgt tcaattttgt aaattgtttg cggcctaaag tgtttttagg aagcatacct
     2461 tttacagaag tttcgatcaa acgacgagaa tttttagcac gcaattcgcc agcagtgatt
     2521 gatttcaatc ctcctgggta gttgctgtga cggtaataaa ttttgtcagt cgctttttta
     2581 cctgttaatt tcactttgtc tgcattgatg acgatgacga aatcgccagt atccaaatga
     2641 ggtgtgaaag ttggtttatt tttaccacgt agtacagatg caacaactgt tgaaagacgt
     2701 cccaatggaa catcagttgc gtctactaca taccatttac gttctacttc gcctggtttg
     2761 gccatatatg ttgtacgcac gttattttcc tccaatttaa gttctgatgt ttgacataca
     2821 cttcgagttt ccggggctct ttgtggggca aacaatacca tttaacatat tacccgctaa
     2881 tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac tttacttctt
     2941 ttctttcttt tatataaaag ggtcgtttcc tgtcaaaa
//
LOCUS       DASJON010000247         2958 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJON010000247 DASJON010000000
VERSION     DASJON010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.253"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..280)
                     /locus_tag="U3989_002706"
     CDS             complement(<1..280)
                     /locus_tag="U3989_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN1716118.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="U3989_002707"
                     /pseudo
     CDS             742..2124
                     /locus_tag="U3989_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2359..>2958)
                     /locus_tag="U3989_002708"
     CDS             complement(2359..>2958)
                     /locus_tag="U3989_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1716119.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT         1015 a          562 c          518 g          863 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaactt atctcattca aggcttgatc ttccaagtaa aacaaaaacc agttaagcac
     2281 tctgcctaac tggttatgac taaattttca gttgtatctt tctctaccaa cactaaatgt
     2341 cgaagagcct tttttcgttt atgaatgtaa gaccgcttct cgagccaatg ccaaacaacc
     2401 gagcgttcca gcgttgtctt ctaatgctgg ggtcacgata tatttgctta actcaggtgt
     2461 ttcgacatac ccattgacca attttgcaaa tgcgtcgtgg acgttttgca gcatatggcg
     2521 ttgtttcatc acaccaccgc caaaaatgat cacatctgga gaaaacatca aggttgtatt
     2581 gtaggcacat tgagcaatat aggaggcttc gatttcccaa taaggatcat cttctgcaac
     2641 ctcttgtcct tttttcccta agcgtttttc gatggcgggt ccagcggcca tcccttctaa
     2701 acagttgcca tgaaatggac agctgccagc aaagtcatct tttggatgag ggacgacgag
     2761 catatgcccc atttctggat gactaaaccc ttcgataaag cgcccttctt gtaaggcacc
     2821 gccgccaaca cccgtaccga tcgtatagta aacgacactg ctcaatcctt tgccactgcc
     2881 gaagacatac tcaccatagg cagccgcatt gacatctgtt gtccaagaaa tcgggatcgg
     2941 aaattctttt ttcatggt
//
LOCUS       DASJON010000248         2936 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJON010000248 DASJON010000000
VERSION     DASJON010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.254"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            251..667
                     /locus_tag="U3989_002709"
     CDS             251..667
                     /locus_tag="U3989_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1716120.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            681..1247
                     /locus_tag="U3989_002710"
     CDS             681..1247
                     /locus_tag="U3989_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN1716121.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1270..2256
                     /locus_tag="U3989_002711"
     CDS             1270..2256
                     /locus_tag="U3989_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1716122.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2411..>2936)
                     /gene="scm"
                     /locus_tag="U3989_002712"
     CDS             complement(2411..>2936)
                     /gene="scm"
                     /locus_tag="U3989_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1716123.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT          875 a          544 c          622 g          895 t
ORIGIN      
        1 ttcacaaaaa cttgaaaagt aattttcgta aattccttct tatttcttga agttgcccac
       61 ttctgtcaca acctcttgtt ataaacggtg ttccacctgc tgcttctcgg taataagccc
      121 aaagaattag aaaacatgga aagctgtttt ctattcttcg ttcttattac tcaaagcaag
      181 acacaggtgt cacaacctct tgtttttatg ctaaaattgg gtgagttaat ctggaaaaag
      241 gagaacaaaa atgagcctta aagaatttgc tgaaaaaagt ctgctcaaac cttgggatgt
      301 ttttattatt ttgcttttga ctgttctttc ttttttacca gtcatgattt ttggtcttca
      361 gcaaaacacc gcaacagcag aaaaagaagc ggtactgcgt gtggatggca cagaaatcaa
      421 aacattccct ttgatggctg gagaagagag caacacatat acttacaaag atgaacacgg
      481 agattataac ttgatcgaga tagatggcga tcgagtacgg ataaaagaag cagattgcga
      541 tgaccagatc tgcgtacgtc gaggctgggc aacgaaaaat ggcgaaacca tcgtctgttt
      601 gccacataaa ttagtgatcg aagtccgttc aacagatgga ggtgacgaag acagtctgat
      661 ttattagacc tttacacacc atgagtaaat tacaaaaaat gatttatatt tcgctacttg
      721 ttgcacaagg agtgatcatt ggtttattgg aaaatatgat cccttatcct ttttcttttg
      781 cgcctggtgc aaaactaggc ttagccaatc taatcacgat cgttgccatt tttaccatga
      841 aaaaacaaga cagcttctta ctactttggc tacgactatt cctgactact ttgctaggcg
      901 gaacgatttc cacattcctt tatagtatga gtggtgcttt gcttagttat tttggaatgt
      961 tattggtcaa gcaattagga ccaaaacgtg tcagtatcat cggaattagt gcggccggcg
     1021 gtttcatgca taatgtcgga cagcttttga tggcttcttt cattgcacaa tcatggacag
     1081 tcatgttgta cttgccagtc ctttcagttt tagggatttt atcagggatt gcaatcggga
     1141 tcgcagcgaa ttatctttta cgacatgtgc agacacttca acgattccaa aaagattatg
     1201 agcagggaac caaacaaaaa tggagcccaa tgtttaccaa aaaataatcg tcacacaagg
     1261 agttttttta tgaagctaca tccaatgtgg gaaaactatc ctgctttaag taaagaattg
     1321 acagaaacac taaaacggat ggaagaaact gtccgcctga aaaacaaacc tgtcgaacaa
     1381 gcaatcaaag aaacgatcca tgccggcgga aagctgttgc gtcctgccta tcagctattg
     1441 ttctcacaat ttggaccaga gcaagatcga gacaaagcca tcgctttggc agcttctatc
     1501 gaaatgctcc atacggccac actgatccat gatgatatcg ttgatgaagc ggatctgcga
     1561 cgtggtcagc ctaatatccg tagtcagttt ggtaatgtcg tttctgtcta tgcaggagac
     1621 tatttatttg tctgctgttt taaactattg tctgattact ccacttcctt gaaaagtctc
     1681 caattgaatt ctcgcagtat ggaaaaagta ttgaatggcg aattagggca gatggatgat
     1741 cgctataatt atgaactttc tgtaaaagaa tatttggaaa atatttcagg taagacagct
     1801 gaactgttcc aattaagttg ttctgttggc gcttttgaaa gtggaacaag cgagcgcttc
     1861 gcaaaaaaag caggagatat cggactttct atcgggatgg cctttcagat tatcgatgat
     1921 attttagatt atacaaaaga gagccaggaa atcggcaaac ctgtactaga agacatgcgt
     1981 caaggggtct attctttacc gttgatctac tctttgcaaa aaaacaaacc aaagctacta
     2041 ccttatttag agaagaaagc ggcaatgaca gaagaagatg tcgacgccgt acgcaaaatc
     2101 gttgaacata caggaggcgt ggaagaagct aggaagttag cggcctctta cacagaaaag
     2161 gctttgaagg aaataaagaa attacctgcc acctctcttc gaacaaaaga gaatctcttt
     2221 tcccttaccc aactgatttt ggatcgcaaa gactgaatag ggaaaggttc gatacgtaaa
     2281 taaatttctc agaaggaaat ctacgtgttt tttgacaaat tgaattgtag ttcttgtatt
     2341 tatgataaaa agcaaaaata aagagcaacg tccgttagcg acgttgctct tttttatctt
     2401 gaatattttt ttaccttttc gatttcttat aactcataaa agtgattctt aaaccgatta
     2461 caagtaatat aacgcctgcg agtgttatcc aattattcga ttgacttcca gttttaggaa
     2521 gtgctctttt tgatttgttt ccattgtcat tgcttgtttt attagttaag ttaaagttcg
     2581 tcctgttatc cgaacttgtt tttgagaagg agacacttgc atgacgtgta tcgcttgtgg
     2641 tagacgacgt gtctgtacta tcgcttgaag ttgatgaatc tgagctatca cttgtggtag
     2701 aggatgtatc tgtactgtca cttgaagctg acgtgtccgt gctatcactt gtagtggtag
     2761 atgaatctga gctatcactt gtggtagagg tagtatctgt actgtcactt gaagctgacg
     2821 tgtccgtgct atcacttgtg gtagaggtag tatctgtact gtcacttgaa gctgatgtgt
     2881 ccgtgctatc acttgtggta gaggtagtat ctgtactgtc gcttgaagct gacgtg
//
LOCUS       DASJON010000249         2903 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJON010000249 DASJON010000000
VERSION     DASJON010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.255"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..144
                     /locus_tag="U3989_002713"
     CDS             <1..144
                     /locus_tag="U3989_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1716124.1"
                     /translation="QTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGNEIK
                     IGN"
     gene            160..1002
                     /locus_tag="U3989_002714"
     CDS             160..1002
                     /locus_tag="U3989_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716125.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1065..2867
                     /locus_tag="U3989_002715"
     CDS             1065..2867
                     /locus_tag="U3989_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEN1716126.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1060 a          389 c          597 g          857 t
ORIGIN      
        1 cagactccac agaatttcaa taagaaatta caacgagaaa cggtaacctt agatgagttg
       61 aaagcgattg ctgatgtgct aggtgtcaag tttgtgcagg cttttatatt gcctgatggc
      121 aatgaaataa agataggtaa ttaggaggtg taatttgtat tgaaaatcat aacaataaat
      181 gatgttgagt atgctgtatt tgctgctaat gaaggtactt ccaaaccaca acctcatatc
      241 atagaaacga aatctggtac tattccagaa ggaaaacaac tgagtttatt aaaagaatat
      301 cttaagcaga atgatatttc cccaataaaa ggagcaacaa catattggtg cattgacaag
      361 gttcttaagc ttgattcaag taaagaaaaa actataagtg aaacaatcca taaacaaaaa
      421 tacttatctt taactgaaga aaatattgag aaacagcata agtttgtagg tgcaagctct
      481 aattatggga aagaaggtct aataatacat gatgttttga atgcatttcc tcttcataat
      541 gatttaaata ctattgcgat gaagattgcg gtaattgatg taacaaattc aacacatctt
      601 tctcaatata agagcaggtt gtctctatat gatttggcta aagtaattct ggagattcca
      661 aattttgatg atcggttggc aaaaggagat cctcagttga taaatattat cgcaaggaac
      721 attggtgcag ttaatatgtt ctcatttgca tccaagtact gtacatatca taatgtggaa
      781 gtgtgtggga gagatgatta ctccatcttt gatggtatcg ttaaaaatac tcttccacac
      841 tatatccaag gactaactac gaataagatt gatacgtggc gaagaagttt tgattacgag
      901 gcatttaacg aatgtgttgg aaaactactt gatgaaaata atatacatat tccttttaga
      961 agaagaaagt tagatcactt cttgtggtat gcgaataggt agaatataag aaaaaggcat
     1021 cgtaaaagtg attctggata aaattaatgg gggaggaaat aatagtggga ttatttgatt
     1081 ttctaaagaa aaaacaagga aaggttagta aacacgttga ttatcaactg gcttctagtc
     1141 tatttcgtac tggtaaagtt tttgctgaaa aattggaagt ggacatatca aatgtagatg
     1201 agtttcgtaa aacttttatc gcttttgatg tggaaacaac tggcctaaat tcttcttcag
     1261 atagaattgt agagattggt gcggtgctat ttaaagaagg agttccaatt caatcattcg
     1321 gaactcttgt caatccaaaa gttagaattg cagcctcagc ttcggcaata aataatataa
     1381 ctaatgaaat gctaaactct gctccaaatg agcaggaagt ttacccccag ttggtagaat
     1441 ttttaggaga tgcactagat ggaaaaatca ctatgtgtgc acacaatgca agtttcgatt
     1501 ttggattttt aagtaacaca ttttctagac ttgggtatga tgctaaaatt aagtatgttg
     1561 atactctgag tctctctaga aaacatataa aaggcattgt aaatcataag caagagactg
     1621 tagcggaata tttcggatta ataaatgaca ctgcccatag agcggaatca gatgcagata
     1681 tatgcggaaa gattttttgg ggtgttttag atattattga ggagtcgttg gaagaacaaa
     1741 aaaagcaaat agaaaagatg actccagaca taaaagaact tgaagcttgt gcttttattc
     1801 aagatattat tgagaaaaaa ggatgcgaca ccagttggat caggtatcga aaaaacagta
     1861 ataattatgt cgatgtaact tgtctatata cattcttaaa gtttaagttt gctaagaagg
     1921 gaaattacat cattattaga aaagatgcta caaatggaat tgatttacct atagaacctt
     1981 gtactgtaag tgaaggcggt acaacaaatg tgcgagtcta ttttcgtagc ccctttgact
     2041 taacgcctct ttcaaattat attttcagtg cttatagcga ttgttataat tctatgaatg
     2101 aatatatgag tgttagtaat tacactaaaa aagaagcaga gcaaagcctt agtatgctga
     2161 aatctatttc aaaatcagcg atgaaagagt tattattcga tgcaaaaaat cgtggatatg
     2221 atgagactga agttaaccta aatattgaaa aagtgatatc tagagaagat gtaattatta
     2281 acgggttgca taatcgagtt tcattaaaca agatacgaaa tatagggaat tgggagaaag
     2341 gatttgatgc aggttatcca tattgggaac gtggagaaac tgctaggaaa aatggcaaag
     2401 tagatgaagc tattgcactt tttgataaag ctcgatataa cggctatgat gctcctgcgc
     2461 tatatgattc ttatgctaaa gcttatcgaa aaattaagga ttacgataat gaaattctca
     2521 ttcttgatga aggaattatg cgtaaaacac ggcacgatgt tggtacttta gaggctcgta
     2581 gggataaggc aattaagcta ttatttgcta aacaagaggc agagcggata gctaaggaaa
     2641 aatctgattt tctaaaagcg aacaaaaaag aggatgcgat tagtgagcct agcaagaagc
     2701 gtggaagaca gattattcaa atgactgatg atggaacaat aataaaagaa ttcgatacta
     2761 tagcttctgc tgtaaaagaa gttggcgtta actcaaaaag tattagggat gctgcaaatg
     2821 gagttcaaaa acatgctgga ggatactact ggaaatacaa agattagcag ataatctaag
     2881 ataacctagg atcttcttaa tag
//
LOCUS       DASJON010000250         2894 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJON010000250 DASJON010000000
VERSION     DASJON010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.256"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..941)
                     /locus_tag="U3989_002716"
     CDS             complement(<1..941)
                     /locus_tag="U3989_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1716127.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWL"
     gene            complement(1031..1711)
                     /locus_tag="U3989_002717"
     CDS             complement(1031..1711)
                     /locus_tag="U3989_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1716128.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(1881..2840)
                     /locus_tag="U3989_002718"
     CDS             complement(1881..2840)
                     /locus_tag="U3989_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1716129.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT          783 a          576 c          530 g         1005 t
ORIGIN      
        1 agccatggga aagattgttc ttgtactgca aaaacggaat tttcatttac tttttgtgct
       61 ttttcttttt tgatcgtaaa gttttgtgta aatcgccatt cattactgtc gcctgatttt
      121 gctatgattt ctgcgcggta atcgccggct tttgctttat caccagctaa atcaagattc
      181 aagtgaaaat tcgaatttgg tgcaactaaa aatgcattag acgtattttc aatataaagc
      241 tgattttcat aaaagatctt gctcgtaaat atcaaattgc tgataatcct aggctgagga
      301 ttttggaaac gaagctgaac agttggcttt gcgttgacct cgtccaaatc tcctgataaa
      361 agtttcagat ccggttctat cgtctcttga ctttcttgta aaacgactgc gagattataa
      421 gcaatacgat ttttgatttg tgctccttct gtagcatttt ctgtttcagc ttcttcttta
      481 ggtgaaacat gaatacccgc taatacacgt cccttaaagc tttttgctgg gattttcact
      541 tctataggta cagttgtttc tgcatgtgct gatacattga tttccttttt cgcaatgagc
      601 aaaacgtcac ggacatcaaa cggcaatgtc tcatctaacg ttgggttttt ttgactataa
      661 tcgatcgttc caccatcact cgttaccgcc ggattgacag agacctcaaa tgttcgttct
      721 tcatctgaag aattgctgat aattacttct agtgtctgat tttgttccgg tgccactaat
      781 aaatcaaaat aaccgattgc tttatctatt tggttctctg gaaataccgg tttgattcca
      841 aagtctcctg ctgaatcctt tgcttggctg attgacggaa aaatgaaaaa tagacttaaa
      901 atagaaaaaa acgaaacaaa cagtcgttta ctttgattca ttgtccacca catcctttca
      961 acaacaactg cggtaaaaaa tccttcacct atagtatttt caccgcagtt tgttgaattt
     1021 atttcatact ctatgctact gggccagcca cgaggttcca agtcaaatca gccgtgaatg
     1081 ttccagctgc tgctttcggc actttcagtg tgatatttgc tggatcataa acagaaagcc
     1141 atgttcccag accttgatct tttgctgctg taaatattgg tgtcgctgca cttgctttac
     1201 tcaacccagt tacagtttga ctagttggtc cagtggattc gttatctgac gtacttttaa
     1261 cagtggatgt tccaaaatct aattcagcgc cttgcaaaac ttgtgaaccg tttacaaaat
     1321 ctgaaaccga aactgtcacg ttccaccctt gtgcttggcc atctgcacct acacctcgac
     1381 gatctgatac ttggagataa ggtgtatcgt tttttgaagc gtctacttga tacgtttttg
     1441 ttccgctttc aatctcgtgt gtaccaaatg gcaattcggg aactacatcc agtgttaaag
     1501 gacctgtctg gccagttgct ggattttccg gatctgctgg ctctactggt tttgaaggat
     1561 cagtagggtt aagaggctcc acggtatttg ttggttcagt aaaggtaata tctccttttg
     1621 aagtcgcttg accctcagca aaagctggtg ttgccaagcc gcctagaaga agtgttgaaa
     1681 gtaataatgt cccaatcatg ttctttttca taataaatca ctcctttttc ttagtgacct
     1741 aagtatagta gagacagtat gcaaaaaatc ctcatgaatt catccgctta cttgaaatta
     1801 tgaccctttt tgtataaaag agaaaaaaac aataaaatcg tttacatgaa tattcttcct
     1861 tttttattaa gtttcgtttg tcaaattaag ttagacaaat catcttttgt gacgtaactc
     1921 aaaaatactt ccattggtgt ccgatagtgt aatgattttc taggaatatg atttcgtttc
     1981 gatgccaccg attggataaa ggtttcagat acaccattaa aatccatagc tttatacaaa
     2041 ccatctttac gtaataagcc atttgaatgt tcattcaatc ctcgttgaga aggtgttcct
     2101 ggatccgcaa aataaattga aatatcgttg gtattactaa tctttttcca atttgagaat
     2161 tcttttccac aatcaaatgt gatggattta aataagtttt ttgggacaca acgaaaccat
     2221 tgattcagcc tattttcaat gtctttagcc tgtctgcctt cgggtttcaa ggtaatgatg
     2281 actttagata gccgttcaac taatgtgatg accgcgcttt tatgttttcc acccacgata
     2341 gtgtcacctt ccaggtgacc aaattcatga tcaaactggg tatagtctgt ctttctgtct
     2401 cggattgacc ggcgaaaccc ttgttttcct cttttttctt tatgaccgtt tggttttctt
     2461 tttcccttca ttggtaaatc gtgagtgtca aaggttttat ctttaaacat tcgatacagt
     2521 gtgcgtaccg aacaagcaat aggaaactct gctcgaccaa taaggacatc aggagcccac
     2581 ccttgaacaa cctttttttg aatgtattcc tgttgctcat caggtaaaac aatcggatgt
     2641 cttccgcagt tacgtttgtt tttcttatat tgtttatagt agctcattgc gcttccacca
     2701 gatttaagaa aacgatagac cttataaata gtttgtcgag aacgttgtaa cagattggct
     2761 acttttgaaa caggttgtga ttgattgaaa taagcttcta tcattacgag ttcagttggt
     2821 gtaagatggt tataggtcat ttgtactcac tccttataat tctttcgtcg gaactatttt
     2881 gagtgtagca caaa
//
LOCUS       DASJON010000251         2884 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJON010000251 DASJON010000000
VERSION     DASJON010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.257"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..139)
                     /locus_tag="U3989_002719"
     CDS             complement(<1..139)
                     /locus_tag="U3989_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN1716130.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NL"
     misc_binding    229..574
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            619..1260
                     /locus_tag="U3989_002720"
     CDS             619..1260
                     /locus_tag="U3989_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1716131.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1279..1914
                     /locus_tag="U3989_002721"
     CDS             1279..1914
                     /locus_tag="U3989_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1716132.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            1932..2747
                     /locus_tag="U3989_002722"
     CDS             1932..2747
                     /locus_tag="U3989_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1716133.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT          944 a          482 c          633 g          825 t
ORIGIN      
        1 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
       61 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
      121 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
      181 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
      241 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
      301 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
      361 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
      421 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
      481 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
      541 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
      601 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
      661 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
      721 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
      781 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
      841 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
      901 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
      961 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     1021 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     1081 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     1141 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     1201 actggtattc taacaatcat aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     1261 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     1321 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     1381 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     1441 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     1501 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     1561 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     1621 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     1681 ctttctggcg gacaaaaaca gcgggtagct attgccagag cattggctat gaatccacgg
     1741 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     1801 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     1861 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     1921 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     1981 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     2041 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     2101 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     2161 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     2221 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     2281 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     2341 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     2401 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     2461 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     2521 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     2581 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     2641 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     2701 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     2761 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     2821 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     2881 gttt
//
LOCUS       DASJON010000252         2876 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJON010000252 DASJON010000000
VERSION     DASJON010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.259"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            633..959
                     /locus_tag="U3989_002723"
     CDS             633..959
                     /locus_tag="U3989_002723"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1716134.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            940..1875
                     /locus_tag="U3989_002724"
     CDS             940..1875
                     /locus_tag="U3989_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1716135.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            2504..2755
                     /locus_tag="U3989_002725"
     CDS             2504..2755
                     /locus_tag="U3989_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEN1716136.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT          905 a          570 c          524 g          877 t
ORIGIN      
        1 aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc gtccgcctcg
       61 gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat aagataaatc
      121 atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg taaaaactta
      181 tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt acccacctgc
      241 acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc attataatga
      301 ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa ggcaccaatg
      361 aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac cctttccttg
      421 caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta tagcggcaac
      481 cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc gcaaacccgt
      541 agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc agtccctacc
      601 aaacctgtac tattttttta cagaaggttg ctatgttcaa aatctttcgt aaaaattgtt
      661 gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca gatgcgaatg
      721 gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc aatgaattag
      781 ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc ggtaaatttt
      841 ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct catccgaagt
      901 acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg gatttgtgat
      961 ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat ccgccagctt
     1021 cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga aaagaatcgt
     1081 gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag tgatgtgttc
     1141 ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga atcatttgat
     1201 gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca agcggcagtt
     1261 gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa ccatattaac
     1321 gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc agaacccttc
     1381 tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc aataacagcg
     1441 attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc gaaacacttt
     1501 atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac aaaatcaaca
     1561 cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc caatgctctt
     1621 ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa agcgaatcgt
     1681 ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat ctggaatgtc
     1741 ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc tgcgaaagaa
     1801 gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg atataccatt
     1861 atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg ttgtcttcca
     1921 tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg tatcttcaaa
     1981 tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct tagcatttaa
     2041 gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc tcctagtata
     2101 taggcagact gccaattata actgattcaa tccacattat gataaaatcc accttttcgt
     2161 ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg agtcttttca ttcagtgctt
     2221 tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt acaaaatcag
     2281 aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg caacagattg
     2341 gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg ctgtttccca
     2401 gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag caattgacta
     2461 cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat caaatcaaaa
     2521 atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg actgtatcat
     2581 aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat tggacagttt
     2641 tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct cgactcttgt
     2701 ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa aatagaaaag
     2761 agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct ttgtggatcc
     2821 attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg tatttg
//
LOCUS       DASJON010000253         2863 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJON010000253 DASJON010000000
VERSION     DASJON010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.260"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            120..1754
                     /locus_tag="U3989_002726"
     CDS             120..1754
                     /locus_tag="U3989_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716137.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(2021..>2863)
                     /locus_tag="U3989_002727"
     CDS             complement(2021..>2863)
                     /locus_tag="U3989_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1716138.1"
                     /translation="SLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQ
                     QITIRDLLYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGI
                     LIKLTNQPYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESE
                     TEIHNELGTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGD
                     NYILSQGNIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTK
                     LANS"
BASE COUNT          961 a          405 c          434 g         1063 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 taggaatctt atctaaaatc acttatataa tgggtataat atctattcta ctttgtacaa
     1081 aaatatttcc atggtttgcc ttacaaaata ctcctttaaa tattattcaa gctccttggc
     1141 gttttatgat attttcaaca ttgttcatat ctttttcagt atctctattt atttctccac
     1201 agttggaaaa attttcttat acaaggagaa gaaattttat actattatta atattagcta
     1261 tttcaatttt aaattattca tcagtagtta acatggtgaa agaaatgaat tttcaaggaa
     1321 cttattctaa tgaacaaatg gaaaataaac ttgaagaatt tgagaatttt gattatgtac
     1381 ctataggcta taaaaaagat caattgcttt tgaaaaacca tcaagttaca gaagatggtc
     1441 aagttattta tagtagcttt tctccaaata aagatgaaat tacatttacg tttaaatctt
     1501 caaaaggcaa aacaatagtt ttacctgtat tccggtataa aggattaacg gttactgtta
     1561 attcaaaaaa tgctacagta ctaaaacaag gaggtccttt tgttacagta aaaagtgaaa
     1621 gtggaataaa taaagtaaca atcaggtatt catacacatt tttttctaaa atctcattat
     1681 ttatttctat aatcacgcta attgttcttc ttttttattg cataaaatta aaaataagaa
     1741 caagccatga atgatttgct agcacatatt ttagcaatct aaagatacta ttttagattt
     1801 aataggccct ttgtcaaata atgttggtaa agagaaatct tagaataatt tggacagaac
     1861 agaaagaaga acacctcttt ctgttctgtt tatttggaat taaagtctgt agcaaaaatt
     1921 gatttttaga tttgtcccac actctttcct acgtttagtc gagtactttt tttaagcaaa
     1981 tgctattcaa agaagtagta tttgctatct tttcatcata tcagctattt gccaacttag
     2041 tagttccttc catcaggtca tagatttggt ttcttagatc agcagtaggc agtttgatag
     2101 ttttgtcagc ttttatattg ctttccatga tgatagcatc ctgtgaatct ttgctaaata
     2161 tagcagatgt atcaaaagag tcgatatttc cttgcgacag gatataatta tctccgctat
     2221 ataccccacc actatatgga tgtccattcg cattttcacg ccataatgag aagagtaagt
     2281 ctttggaaac tagttttcca gataaaactt gtgtaaacag cgtatttaaa tcatagacgg
     2341 acatactgat attaccagtc cctaattcgt tatggatctc tgtttcagat tctgtcagtg
     2401 gtttaaagta atcatcctct ttagacattt gataggatag tgcatgattg gatgaatttt
     2461 tgacttggtc ataaaatttt gtatgctgta atcctaaagg ttcgatgata tcatcggtca
     2521 ataatttttc atacggttga tttgttaatt tgatcaaaat tccagtcaat atggtaaagt
     2581 tcaatgaatc ataatgagag gtctgataat ctgtaaattt taagtgctga atcgcaaatt
     2641 gaacgacttc ttcatcagtc atcggtatgg ttggagaagc agttcggtgc aagcctgagc
     2701 gcatatacag caagtcacgg atagtaattt gctggctgcc agcaatttgt ggatagaatt
     2761 gagccagctt agtatctaaa gaaaggtgcc cacgctcgac ttgttttaaa atcaaaagag
     2821 tagtcaatgc tttttgaacc gaaccaatct gataataaag aga
//
LOCUS       DASJON010000254         2841 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJON010000254 DASJON010000000
VERSION     DASJON010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.261"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            111..2276
                     /gene="acm"
                     /locus_tag="U3989_002728"
     CDS             111..2276
                     /gene="acm"
                     /locus_tag="U3989_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN1716139.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2548..>2841)
                     /locus_tag="U3989_002729"
     CDS             complement(2548..>2841)
                     /locus_tag="U3989_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN1716140.1"
                     /translation="KHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTPLALMHD
                     KEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYENL"
BASE COUNT         1082 a          429 c          550 g          780 t
ORIGIN      
        1 aattttttag ttaaaagatt ggaaatgagt gggggaggaa ttgaagaaag gatttttgag
       61 agatgatata gtaggtttta tttttagtta aaatagggga gatagaaaaa atgaaaaagt
      121 gtagaaatat tattttatca atgttattta ttattactaa tataacatca ctgattcctg
      181 ttcatgtcta tgcggatgca ggcagagata tcagcagtaa tgtcacttcg ttgaccgtgg
      241 acccaacgaa tattacagat ggaggaaaca taaaagtaaa attctcgttt gacgaaaaaa
      301 aacaaaatat ccaaccaggt gattatcttt ggatcaattg gccaagtgaa ggaaatattc
      361 gcggtgaagg attccaaaaa gaaatcccat taatgattga aaataaaaat gttggaactt
      421 taacggtcag aaaagattct gcgcaggttg tgtttaatga aaatattaaa aacctagact
      481 cagtagaagg ttgggggcaa tttgagatac aggccagaaa cgtaaccgat accggtgaag
      541 aaaatacggg accattcacc gtgacgagcg gtgataaaac agctactgta aatgtgacaa
      601 agccagcttc tggttcttct tctagcgttt tttattataa aacaggagac atgctacctg
      661 aagatactaa gcacattcga tggtttttga atattaacaa taacggcact tatgttgaac
      721 aaccagttaa aatcagtgat gaaatccaaa gtggacaaag attagatcca agtacgtttg
      781 aaattaatca aatacactta ggtgaacaga aggtttatag aggagaagag ggaatccaac
      841 aatttttaca agatttcccc agcgctacat ttaatttttc agtaactgat aattacattg
      901 aaataactat accaaaaaac tttgtcaatc ttaggaaaat aatggtgagt tataagacga
      961 tcatagaaaa cccagaacaa ataaattttg agaatcattc ggaagcttgg tttaaagaat
     1021 ttaataaacc tgctgttgat ggagaaagtt tcaatcatac agttaaaaat atttcagctt
     1081 ctggtggagt caacggtaca gttagaggcg aattgaagat tttcaaatat atcaatgaca
     1141 cagagattgg tatcccaaat gtgactttcg aattaagacg agcagatgaa cagcctattc
     1201 aaggacagtc tagtatcctg ttgacttcga atgaacaggg agaagctagt atcaaaggcc
     1261 tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc caattggatt gattttgatc
     1321 cactaagttc aaatgaactc aaattctcca taaacgaaaa tgatacagag ggagtcagtt
     1381 tacctattta taacaagaaa aaggttacga acatcacagc aactaaaatt tggaacggtg
     1441 gaaccactcc aagaccgagt atttatttta aattattcag agcaagtaca aacaattggg
     1501 aaccagttcc tgatgcagaa acaaaacgat tagacaatgg aataacttca gtgacttggg
     1561 aagatatcca acagtatgat gatagtggaa acgaatatac gtttaaagtg caagaagtag
     1621 atcaaaacgg aaatgattat gtcccttcag gttatcaaaa aattgaaaac gggctagtcg
     1681 ttacaaatga gaataaagaa gtcatttcag tctcaggaca aaaaacatgg gaagacaatg
     1741 agaaccaaga cggcaaacga ccgacgacga tcacagtaaa tctattagcc gacggtcaac
     1801 cgatccaaca caaagaagta agtgaaaaag atgattggcg ctatcgtttc acaaatctgc
     1861 caaaatacag agacgggcaa gaaatcatct atactgtaac ggaagacaac gtgcccgaat
     1921 acagtacgac gattgacggg tataacatca aaaacagtta tacaccggaa aaaacaaata
     1981 tatctatacc aggtagccat ataacaccgt ggaaaaattt tccagagaag acagagagag
     2041 agaaaattag aaatctattc tctcaaaaat tgcaaatatt tgctcataca ggagaatcca
     2101 gcgaacaaag aagcaattat attagtaaga gagctgtacc taaacagata acaaataaat
     2161 caaaaagcat tcttcctaaa acaagtagta aaaaatctag ttttgctgta atcattggtt
     2221 tattgatagt aactatctgt gctgggatat tcttacaaaa atataaaaaa gtttagttag
     2281 aatataatat tttttgttaa atatgactga aatcaaaaga ataaaattcc gatttataat
     2341 cgaaaaaaga agcattttta tatatgcttc ttttttcgta gaatcaataa atatggattt
     2401 ggctaactat ggaagaatac tcgtattagt tttcagcgtt catactagtt gttacattgt
     2461 ttgattggcc aaaagaattt ctgatatatt ttgtgctaaa taaagaaaaa gacgcttgaa
     2521 ttgggaaata agaaaactac tctatcatta caaattttca taaactggca gatgttctaa
     2581 gtaaacaagt atcttttcaa agtcggaaaa atccataatc aacgtagtgg tattgatatt
     2641 tggatgaaag cctacggtat cttgacgatc aatagcagca tcgatcacga cttggatttt
     2701 tttctctttg tcgtgcatca atgctaatgg agtcactgta cctgctggaa cgcctaggag
     2761 gtcaggaagc tgttcttcag aaacaaaaga taagcgcttt tcttttaatt gttcagctag
     2821 ttgttttaaa tcggcgtgct t
//
LOCUS       DASJON010000255         2768 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJON010000255 DASJON010000000
VERSION     DASJON010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.262"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="U3989_002730"
     CDS             <1..1152
                     /locus_tag="U3989_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716141.1"
                     /translation="KQRELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKR
                     SQQKLYCECGRELKVQYIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDL
                     ALDELLWLKQKNIDFPEGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIY
                     IADYQALENEIKKISEHINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDW
                     QSIVYEEVPVHPNAYFETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWK
                     QYCRYGFTEGFFDSIPRIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQS
                     GNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            1410..2627
                     /locus_tag="U3989_002731"
     CDS             1410..2627
                     /locus_tag="U3989_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1716142.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
BASE COUNT          944 a          549 c          535 g          740 t
ORIGIN      
        1 aaacagcggg aactagtcga taagcaccgc cgatatctaa tcagctctcg ttttatggaa
       61 gaacattatt tagctgcaac agattgggtg ttttctgact tcaagatcaa tccgtttttt
      121 cgaacaaaac gaagccagca aaaattgtat tgtgagtgtg gtagagaatt aaaagtgcaa
      181 tacatcgtta aatcgccaaa aaccggaaag atcctaaaac taggcatcaa ccactttgcc
      241 gatcacctgc atgtctctcc gacagtggca gcttcgatcc atcagggaat gacaaaagtt
      301 gatctggcct tagacgaatt attatggctt aagcagaaga atatcgattt tcctgaagga
      361 ctatggcaaa aatactgttt tgtcttgtac caaaatcgtc ggatgaaaca gccatattta
      421 ccggatataa aattagctca gcggctcgca gaatttcgcc aagtagaaat gccgatttac
      481 atagcggatt atcaagcatt ggaaaatgaa atcaagaaaa taagtgaaca catcaatggg
      541 cagtcaaaaa agcgacagat aaaaaaagaa ctatttgacg actttgcaga agaattagta
      601 aaggatgtag aagaattctt gatcaactat cgcgcatttt tacgtaaaga ttggcaatcc
      661 atcgtatatg aagaagtgcc agtacatcct aatgcttatt ttgaaacatt tatttccgtg
      721 ttgagaaaga caaaacgtca acgaacacca gaagtcactg ctcagatgga atattttgcg
      781 aaaaaccaac gattcatcca accaaaaatc tatctattta tttggaagca atattgtcgc
      841 tatggtttta cagaagggtt cttcgacagc attcctcgga tagtcagaaa tggtttttta
      901 aaagtattgc gtaaagaacg agaagctata caatctgctg ataagaaaga ccgtacagtc
      961 tcaaaagaaa aatggcaact agtagtgaaa gatatccaat ctggaaatgt gcaagagaca
     1021 atcgacaaat ggaaaggaaa acattatcgt tttactgaag ctcagaaaca agcgttggaa
     1081 tattatcaga agttagaaga atcactgcgt ttcaatgatg aggcaagaaa gtatctgaag
     1141 gaattactat aaaaaacata ctaagattag aggggaacat cattgacgaa tgatgttctc
     1201 ctctattttc tatttttaga ttatagtgaa gagtgaaaga aagttcgaac aaagccttga
     1261 ggactacgag tccctatcaa aaaccgaact tctttactgt cattttagaa tcaggtttac
     1321 gtatgttggg gccatgagcc cgtgtatagg aaattggaat cgaaatgaag cagtaaagtt
     1381 ctttcaaaac ttaaaaagga ggaaaacaaa tgccttacat tcttgcatta gatgtttcaa
     1441 tgggacacag ctacagtgtc ctatacaaag acgacacctg cttatttgaa gacgagattg
     1501 aacacactca aagaggattt catgcattac tcgaagagat ccagcacttg cctgagcgtc
     1561 cattgattgt ttttgaagca acggggattt attccagacc agtcgaaaag ttctttcaag
     1621 atcatcactt ttcttactgt ttgttaaacc cgttagaggc gaaaaaacag atggaagaaa
     1681 gcaccttacg cagttggaaa acagataaat cggatgccca ccggctcgca cagactcatc
     1741 ttaaaaatca acgtcagcca aaagaagtac aaagcaattt ttacttagag atgcgcgatt
     1801 tagcccgttt ttatcaagaa attgagaaga aaatcactcg tctgcgcatg gatttacaca
     1861 actgccttca attgactttt ccagagcttg aacagttctt ttcaaacagg ctgacccctt
     1921 acgcattgac attgatccgt ctttttcccc atccagattt tgtattggca tctactcgga
     1981 caaaaataaa aaataaactg atcaacgaaa cacggaaaaa aatctccgcg aatcgtgccg
     2041 agcaaaaggc agatcaaatc atccactatg cacagtgtgc ctatccagct gttgaaaaag
     2101 acagtatcca ctgtcaaaaa acgatctatt acgccgaact tctccaagat ttactcgaac
     2161 aaaaaaaagc actggcgaca caaatgatca aaaaagccga aggctctcct tgctttttgt
     2221 tgtaccaaac gttccctggg attggcgcac tcacagccgc tttattgctc ggtgaattag
     2281 gagatatcac acgatttaaa acccataaac aattgaatgc ctttgtcgga atcgacatcc
     2341 gacggtatca ttcaggtaag tatactggcc aagaccgcat caataagcgt ggtaatccca
     2401 aagcacggaa aattattttc ttcaccattc gaaatatgat tcgccagcaa cgcgcagctc
     2461 ctaatcatat tgtcgattat tactacaaat taaaaaagca acctatccct aagaaagata
     2521 aggttgccac agttgcttgt atgaacaagc ttctgaaatg tatgcacgct atggttaggg
     2581 cacatacaga gtacgactat gcgtacgcgg tctcagtgga ccattaacta aaccacttac
     2641 agtataccac ttctttgccc agatcgaaaa ttttttacga acggggcttt atttggtgtg
     2701 ctttgatttt gtcgctttcg tttacgagct atttttcttg caccttttac ttgactaatc
     2761 gtaggaaa
//
LOCUS       DASJON010000256         2690 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJON010000256 DASJON010000000
VERSION     DASJON010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.263"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1987)
                     /gene="topB"
                     /locus_tag="U3989_002732"
     CDS             complement(<1..1987)
                     /gene="topB"
                     /locus_tag="U3989_002732"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1716143.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKL"
     gene            complement(2161..2571)
                     /locus_tag="U3989_002733"
     CDS             complement(2161..2571)
                     /locus_tag="U3989_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1716144.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
BASE COUNT          667 a          576 c          469 g          978 t
ORIGIN      
        1 acaatttgaa gtcgcattcc ggattcaagc atgtagcgat tttaggataa agcatgacta
       61 ttcccttttt acattttgga catcttccta attgatcgtt tttcttctcc atcgctttta
      121 tctgacgtgt cccgctaatc tcatctgaaa agtctacctt gtcgatttga ttaggaactt
      181 ctgccaataa atgtaggatg aattttttga tattctctaa aaaaacagat ggtgtcccct
      241 cttgtttacc aatttttctc aagtaccctt cccagcgcgc agtcatttct gcgctcgtca
      301 gtaatggttc attggctact gccttacaaa gagtgattcc ttttggcgta acagttattt
      361 cgtttttatt gacttcgatg taatgcttct gcttaagatt ttcaatgata ttggctcgtg
      421 tagcttctgt ccctattcct tctacttctt tgagaatttc ttgcgcttca tcattatcca
      481 gcgtttttcc cgcagttttc attgctgtga tcaacgttcc ttcggtatag ggttttggag
      541 gttgtgtttc tttctccata cttttcagtt ctacttctgc ttcttcattt tctacgatag
      601 gaggaagagt ctgagcttct ttcttttttg acttgatgcg taagatttcc tgccaacctt
      661 gctgtaatgg gatctttccg atgcttttta gctttaattg ctgtacgccc gtcattacgg
      721 tcgtctcttc gtagatataa tctgcagcaa acatagccac agtagtgcgc aaaacttgca
      781 aatagattgc ttgctgtaat ttggaaagct tcgcaaagcg tgcaggagtc ggaatcgtct
      841 ttgtcggaat aattgcatga tgttcttgaa cttttttcgc atcgacatat cgtttctttg
      901 ctttgatctc agttgtctgt acttcttcta ttcctaaaaa ttttttataa ttttgcagat
      961 gtttaaccag atactgatgc tcattttctg taatgtaaaa ggaatccgtt cttggatagc
     1021 ttagaaactt gctttcgtac aatccttgag cagcatcaag tgtagctttg gcacttgctt
     1081 tcattagctg attcatcttt gtctgcaaac tagatagcga aaataaacgt ggactatgca
     1141 acctcttttc ctttttcgta acttcaagaa tccgtccgtt ttgcttacct aaatgcgctc
     1201 caagtttttc tatttccttc gttaactctt cttgggtttt aaatgctttt tttggtacga
     1261 tttttccaga aaactgccct ttgtttgtcg taattaaacc ttctccttca aagtacgttt
     1321 cttttttgaa gtgtttgatt tcttcttgca gctgataaat catatagagt gtcggtgttt
     1381 gtacacgacc aagagaaaaa ctgccatcaa tcccttttcg ttgaagtagc aagctatata
     1441 atggacttcc attcattcca atcagccaat cagcgttctg ccttgcttgc gcttctttat
     1501 attttggaaa atagttcttt ccaggttgga gattcttaaa accttcatag attgcttctt
     1561 tttcaagaga attgatccat aaacgtaaat agttcttttc cttagaaaat gcgtttgctt
     1621 gtcgaataat cgaccacgcg atggcttcac cttctcgatc accatctgtt gcaataatga
     1681 tggtatcggc tgactgcaat tctcgtttta cgatcgaaaa ttgtgcacgt ttgtccattg
     1741 gaacttcgta acggaaggtt tctgggaaaa taggcaaatt ttctaaagac cattttgccc
     1801 aacgctcttc ataagctcct ggagcaacca tatctaccaa atgtccaaaa ccataagtga
     1861 tggttacttc accagcaaac aatggatcgc tgatttcata atacccttca tgccgctttt
     1921 gctgttggaa ggtttcggca taggcttttg cttgtgaagg tttttcagct agtatcaccg
     1981 tcttcatctt ctgacctccg ttacttaatt ttttgcggac ttctctatta tggaacatat
     2041 tttcgcatct ggcaataaaa aaaagatacc gtatagtcga aatagacaca aaaacatcaa
     2101 gcaaacaaac gatgatccgt ttgctgcttg atgtttccag aaaatattca tctttagctg
     2161 tcactcttgg ttaatctgcc agttgatgcc aaatcgatca gttaccatcc catacaactg
     2221 agcccacgga acagcatcca gtggcatctc aatcttacca ccctcagcta agctttcaaa
     2281 taggcgcttc gtttcttctt catttcttgt ctggatgact aaagaaacat tatttcccac
     2341 aactaattca ggaccaattc cttctggcgt atctgcaaac ataactaatg tatcataaat
     2401 gttcagccgt gcattcgtga ttagattttt tgcttcttct ggcaagttca tttgttctat
     2461 tccaggcatt tcaccgtatg tggtgatacc tgatgcttca gtctgaaaaa ctttttcata
     2521 aaaagcgatg gcttcacgcg actcattttt gaagttcaga tagatatcca atgtaaacag
     2581 ctcctctctt ggttaaatct accataagaa agcgtttttt acgagtgaat cgtttgattg
     2641 attcatgaca aaagccttgc gcagcttctt cttgatcttt tttatgatca
//
LOCUS       DASJON010000257         2639 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJON010000257 DASJON010000000
VERSION     DASJON010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.264"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..210)
                     /locus_tag="U3989_002734"
     CDS             complement(<1..210)
                     /locus_tag="U3989_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1716145.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVI"
     gene            336..1540
                     /locus_tag="U3989_002735"
                     /pseudo
     CDS             336..1540
                     /locus_tag="U3989_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1535..1942
                     /locus_tag="U3989_002736"
     CDS             1535..1942
                     /locus_tag="U3989_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1716146.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(2183..>2639)
                     /locus_tag="U3989_002737"
     CDS             complement(2183..>2639)
                     /locus_tag="U3989_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1716147.1"
                     /translation="QHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVFLSAIR
                     RQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLNNHIKV
                     LKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          854 a          394 c          531 g          860 t
ORIGIN      
        1 gataacgtcc gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgatt
       61 agtttttttc ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc
      121 gttgccaatt atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttt
      181 gagtatagta agatgacaat actggctcat aattattttc cttctaggag tgttctcgac
      241 aaacttatac tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta
      301 aattcaaggt atagaaagag gttccctatg aaaaaatacc tattacaatc ttaactggct
      361 atcttggttc aggaaaaacg atacttctaa atcatattct gtacggtgat cacggaaagg
      421 aaattgctgt cattgtaaat gacatagggg aagtaaatgt tgatggggta ttagtcgaca
      481 agaaaggttt taaaagaact ggagaaaaac ttgtttcgat gccgaatgga tgcatttgtt
      541 gtacattacg cgaagatttg attgttgaac tagatcaatt agtgcaattt tctgacttga
      601 atcaaattgt tattgaagct tcaggaatca gcaaaccaat tccaattatt caagcaatta
      661 tattagctaa aaacccttta gacattgatt tgacccaacg attagagatt ggtgccatta
      721 ttatagtagt ggatgtaaaa cgtttttggg ataatttcga aacgggtgaa aagattcagg
      781 aaagaaaagc tgaaggtaat gtagttgaag aagaacagga tattcgtggc ttgttaattg
      841 atcaattcga gtgttgtaac atattacttt taaataatag tgacttagtg tcaaccaaga
      901 cgatttacaa aattaacggt ctaattgcta agttacagtc caatgccaaa ataattagga
      961 caattaaagg tgaagtagac ttatcgaaaa ttctaaatac aaatcttttt aatatagatg
     1021 agacttccta ttcaatgggc tggttcaaag agttagagct tggctatcaa aatcatattc
     1081 ctgaaacaga agaatatggt atctcaagtt ttatctatcg aaatcggtta ctccttgacg
     1141 cacaaaaatt tgcccatctt acggataaaa attttcctgg caatattaca agagcaaaag
     1201 ggtttctttg gtttgcccaa catagtgatt tttcgacgat ctttgaaaca tcgggtagcc
     1261 ataaaagtat tgaatcatta aattattggg tagctagttt acctaaaatt gagtgtcaaa
     1321 tgattataca aaaaaacccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat
     1381 taataaacta gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga
     1441 gggttgttta actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg
     1501 gaaaaattct aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt
     1561 caagataggc gatgtcataa ttattggcat tttgattatt ccctctttta ttcccttatt
     1621 tgtattctct atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt
     1681 tcaaattgat ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt
     1741 gaattatcat cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg
     1801 agttaaagaa gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc
     1861 cggtcaaacg agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag
     1921 caaagattac tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga
     1981 tattattatt aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa
     2041 aagaagcggt gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca
     2101 gtcggttaga ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag
     2161 aataatcgag atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg
     2221 ctttagtcag ttgggtagtg ttcttagttt tcttgaaata ttttcctcga tataaaaaga
     2281 ttcgttctcg gtagttttga aagtttctaa agccataggc aattcgtttt agtactttga
     2341 tatggttgtt caaacattct atagggccgt tggaataggg aactcgcaac gctcgtttga
     2401 tttctgttcg atatttcttg aaggtcgtga acactggttg atagtgctct ggcaactcct
     2461 tgtagtcttc ttttaggagc gcgacgaatt caggaacatc ttgtcttctg atagcggaaa
     2521 gaaaaacctg gtagacttcg tagcctcgct ttaaggagtc atcataatat aatagccgat
     2581 cgactatctc cgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctga
//
LOCUS       DASJON010000258         2621 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.265, whole genome shotgun sequence.
ACCESSION   DASJON010000258 DASJON010000000
VERSION     DASJON010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.265"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(28..972)
                     /locus_tag="U3989_002738"
     CDS             complement(28..972)
                     /locus_tag="U3989_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1716148.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(956..>2621)
                     /locus_tag="U3989_002739"
     CDS             complement(956..>2621)
                     /locus_tag="U3989_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716149.1"
                     /translation="HWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQIAKEQ
                     QEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLKVILL
                     LIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKRTGEE
                     KQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQEKLR
                     QIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEINQLKK
                     KQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPEELTK
                     EKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQKAKL
                     QELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLKNDTL
                     TLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQRKYQ
                     LAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
BASE COUNT          683 a          532 c          436 g          970 t
ORIGIN      
        1 gttttgaatg aaaaaaataa atttttgcta taaatctttt ggtacataga aacttcggtt
       61 gtccataccg aaaatccgat cggtgtattt tcctggttct gtttgacgaa tcgaagtcaa
      121 catcatctgc atcgaagcat cgatattgtc gatttgatgt aatatttctg cttccattat
      181 tcttggacgt acaggtgagc catattctaa taagccatgg tgtgctaaaa ccatatgacg
      241 taatacgaga acatcttcgt cagcttcgct gattttcaac gcaagacagg ctttagttat
      301 ttcttcgtca atcaatacca gatgaccgat caaatttcct gcaagcgtat attctgtcga
      361 cataggaccg gaaagttcaa tcacctttcc taaatcatgc aaaatgatac cagcatacaa
      421 caatgctcga ttcagctcag gatattcatc ggcgattgat ttccctaagt gaagcattgt
      481 caaagtatga taagctaatc cactggcaaa tgcatgatgg tttctttttg ctgccggaaa
      541 ctcaaaaaat tgtttttgat atttattaag tagatagcga acaatacgat tccaatgcgc
      601 attggtgatc tcaaacaaca attgattgat ttcttcttcc atttcttctt tcttcaaagg
      661 ggctctttcc atgtataaac taggctctcc tggctcatct acccttgtta agcgcatatg
      721 aagaatcttt acttgagggt ttccttgata aacctctctt ttaccgttta gaaaaacgac
      781 tgtgccggct gtaaatttat tgatttcctc ctccgaagca tcccagtatt taccatcgat
      841 agtacctgaa gtatcttgga aagtaaaagc aataaatttt tttccgtttt tagcgattct
      901 tacatctgca cttttgatca ggacaaattg ttcaaactgc tcatcgatta ctaaatcacg
      961 aatttttttc attatttgct ccttctaatt gttttacgat ttggttcatc tcatggtaat
     1021 agcttaccat ttctttatca gaagaaaaac aaattatttg atacttttct ccaaatttag
     1081 ctaaaagttc agctaattga tactttcgct gatgatcata atgaagccag ccatcatcaa
     1141 tgatgatcgg gcatatcgaa cggttagctt ctaatgagag gtaagcaaat ctcatcgcca
     1201 taatcagctg atctttcgta cctgtagata actcatagat gggaaattct gtatatccgt
     1261 ctgttaaagt cagtgtatca tttttcagta atatcttctg ataatttcct tttgttaaaa
     1321 tacctaaata atcacctgct gctcttaaca actggggtaa ttgtctttca ctcatctctg
     1381 ttgaaagatc acctaagaat gtcccggcga tttcaagtgc tccccattcc tccagtaatt
     1441 cctgtaattt tgctttttgc cggctagctt gttgataaag ttcgtccaat gtgccgtctc
     1501 tttgcaactc ttctacttga agttggagtc gttgccgatt ttcacttgct tctttttctt
     1561 cttgctggag ttgggattgc ttttctttca gttggcgcag tcttacagat aatttctctt
     1621 ttgtcagctc ttcaggaaat agtggagtaa gcatttccga taattcattg tatcttttca
     1681 tctgctgttc ggcttgtttg gctttttgta aaaagacagg tacttccgaa ggataagata
     1741 agcctgcttg agtcaattgt tcgctgtatt cttgtatcca tactttttgt ttctttttca
     1801 attgattgat ttcttgcttc aataaagtat tttgctgata gcttctggca aatttcatct
     1861 gttccatctc ttgtataaaa cgggaaagca ataccatttt ttctgaaatt gtcttatctt
     1921 gtatcggtaa ccattcttta acaaactcaa atttttggtc gatttgtcgt agtttttctt
     1981 gtatttcctg ttctctttta tgtttccgct caaatacgcc atataatttc tgataatcta
     2041 tgacttcctg tccatattct ttcatgagtt tgagcgtatc catctttcct aagtgccgat
     2101 gatccgcaaa acgatatact tcttgctgaa gaatctgttt ttcttctcca gttctctttt
     2161 gtttttcttc tgcttcagct aattgagcta aggctagatc aagttgtcct agtttttctt
     2221 gccacattcc ttttatctca gagaaatcat ttttagtttt aatttttggt tgccttttgt
     2281 agaagaaata gccaatcatg cctgcaccaa ttagtaaaag gatcactttc aagggcattg
     2341 gtacaaatat acctgcaatg attattgctc cgccagccat cagcatatac ggtttagatg
     2401 aagagactgg tgctatctct gactgatttt gtaataatgc tggataggtc tcttccaagt
     2461 cattcacttc tgcttcaatc atctcttgct gttctttagc tatttggaga cgcacagaaa
     2521 tctggctttc tagatcagca atccttcttt cttcagccag aagatgcttc cattcttctt
     2581 catataaaaa ttcttccggt aacggatttt tctgccaatg c
//
LOCUS       DASJON010000259         2619 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJON010000259 DASJON010000000
VERSION     DASJON010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.266"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..66
                     /locus_tag="U3989_002740"
     CDS             <1..66
                     /locus_tag="U3989_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN1716150.1"
                     /translation="QLMVQEKDKICSLPGGEWIDD"
     gene            56..2227
                     /locus_tag="U3989_002741"
     CDS             56..2227
                     /locus_tag="U3989_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEN1716151.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(2339..>2619)
                     /locus_tag="U3989_002742"
     CDS             complement(2339..>2619)
                     /locus_tag="U3989_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN1716152.1"
                     /translation="GAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWG
                     AGYNILAIPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT          808 a          526 c          491 g          794 t
ORIGIN      
        1 caactcatgg tccaagaaaa agacaagata tgttcattgc caggaggtga atggattgac
       61 gattaaatgg gaaaaatggc tgtgtggcgt ttactccttt gtgatactaa gtgtaggtgc
      121 tccgatattt gtagtagcta atcaattgca aaacagctac cttcttatcc taactattgg
      181 tgtcctatgc cttattgtct gctgtattcc taaaaaatgg gtacgtttgc tgctttcttt
      241 gccagtaatg ataggttgtt tgtttttgta ttttccttcg aagacatttt ccttattatg
      301 gatcttacag tttttttcgt cagtcacaga ggaattcttc catctatccc aaggtaaact
      361 ttattatttg ccagaaaaga cagctttttt tctcataatg ttgctgatct atttatttat
      421 tacattgacc gtggattacg actatttgta cgcacccttt ctttctttga tggcttactt
      481 catgatgctt accgtttttc gggaaaaaga tttactgtac gaaatgattg cggtagtaac
      541 tgttttgttc gtgtatctgg cagcaaggca atttttctcc atgcggttgt tgtcgggaaa
      601 cactcgattc cagtcattga ctacagttct gcttatttgt ttcttaacta ttagcgctat
      661 cttgccttta tatcttccga aagtacacca atctctcgag gaaacgagtg aaccgatccg
      721 agaatatctt aatgatgaag ggctttatga tacactccat gagtatcaat taaccggaag
      781 tgcaagaaca ggcttcagtg agaatgatga acagcttggt ggacctttat atgataatgg
      841 cgaagttgtt tttacagcga accaaaaagg aaatggctat tggaagatcg aaaataaaaa
      901 tcattacacc ggttctggat ggatagaaga tcaggacgca cccatccaac caatccaaga
      961 attccctgtt gacatcacga gctaccaaac cgttatggaa gacaaagaaa acattacatt
     1021 gaccctaaaa aaacaagctt tcttacctta tccttatgga aatactacct tgactgattt
     1081 ttcattagct agtggacaaa cgctttacta tttgcctgaa agccagcgat tcattcctgt
     1141 cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa aggttagttt ttacgagaga
     1201 agaattaaaa agcagtcagt taaaagctga tgatttagaa aatcagcaga caaatcttca
     1261 attgccaaaa agtttgccta aacgtattga ggacttggcg gtcgaattga ccaaaaatga
     1321 agcaacttta attcgtaaag tcgaagctgt cgaacagtat ttaaaaacaa gcggaaattt
     1381 ccgctattcc aaaacggata ccggacatac tccgaaagaa cacgattatg tggattattt
     1441 cctctttgat tcaaaagtcg gctattgtga caacttttca acttctatgg tcgtccttct
     1501 tcgaacactt ggtatcccag ctcgctggac aaaaggtttt acaaatgggc tcagaacagg
     1561 cacagataat caagggaatg aggaatacag cattctcaat agtcatgctc attcttggcc
     1621 agaggtatac tttcctggat ttggctggct tccatttgaa ccaacccctt catttgccaa
     1681 tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc gtccagcctg ctatcgaaca
     1741 accaaatgaa agcgaaaata tggcagaaac tatttccagt gagagatctg aggtcattcc
     1801 atcagaagaa acaacactca caccagctta taaaatcgtc ttattcagcc ttggggtgtg
     1861 tttatacgtc ttgttgttct ttctattgtt attcagaaaa tctctgcgct ggacaagtta
     1921 tcttatctat cttcattttt ttgcttctac taatacgatt gcatatcaaa aacttcttaa
     1981 ccatttggaa acattggtac cgcgcaaacc cgcagaatct ttgcaggaat atagcctgcg
     2041 aatagaacaa gaattcaagc tagatctgcg tttcattcga ttaactactc aatatgaagc
     2101 agaaatatat ggacgacaag cagatccggt aaatttcgat aaaaaaacct atttagaaat
     2161 tacgcatata ataagaaaaa cggtatacaa aaaagttctg acaaaaggga gccgcttcaa
     2221 aaaataagat aaaatttctg gagaatctac ttctggaatg ccgagaacca gctgcttgag
     2281 tatcgattat tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt
     2341 atttgatttt caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga
     2401 cagcaccaac tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg
     2461 gaattgcaag aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg
     2521 tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga
     2581 catccgcaga atcgattgcg atatctgtcc ctgcaccga
//
LOCUS       DASJON010000260         2587 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJON010000260 DASJON010000000
VERSION     DASJON010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.267"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..386
                     /locus_tag="U3989_002743"
     CDS             <1..386
                     /locus_tag="U3989_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1716153.1"
                     /translation="LKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQDYRNE
                     MDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKKYSKEK
                     AGGFMVYKGITLKPRKYDNVRELFK"
     gene            633..950
                     /locus_tag="U3989_002744"
     CDS             633..950
                     /locus_tag="U3989_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEN1716154.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            947..1108
                     /locus_tag="U3989_002745"
     CDS             947..1108
                     /locus_tag="U3989_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1716155.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1111..1410
                     /locus_tag="U3989_002746"
     CDS             1111..1410
                     /locus_tag="U3989_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716156.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            1410..1724
                     /locus_tag="U3989_002747"
     CDS             1410..1724
                     /locus_tag="U3989_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN1716157.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1717..2247
                     /locus_tag="U3989_002748"
     CDS             1717..2247
                     /locus_tag="U3989_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN1716158.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            2244..2513
                     /locus_tag="U3989_002749"
     CDS             2244..2513
                     /locus_tag="U3989_002749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716159.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            2443..>2587
                     /locus_tag="U3989_002750"
     CDS             2443..>2587
                     /locus_tag="U3989_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN1716160.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGA"
BASE COUNT          958 a          378 c          605 g          646 t
ORIGIN      
        1 atctgaaatt taaattgcag acggagttgc aagggatcct taaatgggca atcgatggtt
       61 gcctgttgtg gcagagagag ggcttggaga aaccaaagtc ggttgttgca gcgagtcagg
      121 attatcgaaa cgaaatggat caaataggta catttatcga aacgtgttgc gaaacaggtc
      181 caggactaaa aatatcaggc ggagaattat acaaagttta tcgtgaatgg gcatcagata
      241 atggcgagca tactttcact aacacgaagt ttggcagaga gatttcaaag aaatatagca
      301 aagaaaaagc tggtggattt atggtttata aaggaatcac attaaaacca agaaaatacg
      361 ataacgttag agaacttttt aaataaatct ggagggttct ggagggttga gaacgaaacc
      421 ctccaaccct tagaaacgtt gatatatcag tatttatatt atatttttct ttctttctgg
      481 agggttttaa aaaaataata aagtattaaa gtaaaaaata aaaagtaaat tatataaaaa
      541 agaataggtt tttgggtctg accctccaaa ctctccacca aaatcttcct aaatgctttg
      601 ataccaacga ttaaacagtc tggagggtta cattgaaatc agaacatcaa atacaagacg
      661 agatcagaat tgccttatca aaacatggtt gcacgatttt tcgatccaat gcgggacaag
      721 tgacaacgat cgaagggcgg caattttacg ggatgccaaa agggttccca gatctatgtg
      781 gacatcgtaa ttctgatggg aaagcaattt atatagaagt aaaaaatgaa cgcggtaagt
      841 tgcgtgaaga tcaaaaaagg tttgctgaat ttttaaacgg tcaacctgta ctatacggtg
      901 ttgcacgatc agtggaagaa gcattgaaaa ttgtggagga atcatcatga caacagaaga
      961 agtgattcaa atgcgtattc gaagccttca gcgtgagatt gacgatctgg aacggacaaa
     1021 ggcagtgatg gtcaatgaaa cggcgaggaa ggcaatcgat ttgcacatag agaatttaag
     1081 aagggaaatc catcgattgg aggaatgagc gtggataaga aagcagcaat gaaacgaatc
     1141 atcgaactga cacattctga gaattggcaa gaagacaaag aaatagttgc agaagtccaa
     1201 aagctcggta aatcaatgtg gagtgaaaaa accaaacgga gaacaccgag aaaaattgca
     1261 atctggcatg gtgaccgaat tctagtaaca ggtactgctg aacagttatc tgaaattact
     1321 ggattaagca aaaacattat ctgggataga gctaaacgcg agaacgttga ttcgaaaggt
     1381 cgtcaattta aacattggga gaagaaataa tggaggaact aatcacaaaa gtagagcagt
     1441 gggcaaaaga taagggattg gatcaagctg attccagcaa gcaaatgtta aaaacgatcg
     1501 aagagattgg ggaagttgcc gcttctctag ctagaaaaga tgaatatggt ttaagagacg
     1561 gaattggaga cgtagtagta accttgatta ttttagctat gcaaaatgat atggatttgt
     1621 acgagtgtct gaaccaagca tacaacgaaa tcaaaggacg cacaggaaaa atggtagatg
     1681 gtgtattcgt gaagtcgagt gatttggagg acagcgatga ataaaaaagt attaattgat
     1741 aaacaagcat tgattgatga attaatgaaa atacctggtg tgggatctaa tagtgacgct
     1801 ttagaaacga ttaaacgttt cccatcgtat gaaccgcaga agccagttgt gcctaagttt
     1861 gtggcggaac tactcgacta ttatcgtcag tcaacagatg ttgatttatt agctttattg
     1921 ataactttcc atgattggta ttatcgcaaa actaaagacg gtgagcatga agaagcaatt
     1981 gattggctag ttgatcatcc tgaaatttat atgcgtgcat ggctgtttgg ctacgagctc
     2041 gagaaagagc cgttgtattg cgtaataatc aatggtagat atcttgtcaa agtattcagc
     2101 aatacaaacg ttgtcatatt agttccagca gatgaatttt cacagtacgt tactcaaaaa
     2161 tttcaattaa ctgaaaaaca aatcaaagca attgatgaaa gatactggcc gtttgctgtg
     2221 ccagtggatg aggtggtaga aggatgagcg acccatacat tactaaatta gaaaagttgt
     2281 ccgaagattt tgagaagaat aaaaataaac taactaaaga taaacttata gaactggtta
     2341 ggttggttat cagaggagct attacaagag aaaaggcgca gcaaaacata gaaatttttg
     2401 ctcgtagtgt ccgagatgga ttagagaaga aagttaaaca aaatgaacaa cagacaccgc
     2461 agaataacaa aactaagaaa acgggaactg aatgtactaa agacaaagtt tgaaaaagaa
     2521 tatggaattt cagcagaaga aacatataaa gtggcaagtc agtgtgttgc tgatgcgagc
     2581 ggcgcta
//
LOCUS       DASJON010000261         2584 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.268, whole genome shotgun sequence.
ACCESSION   DASJON010000261 DASJON010000000
VERSION     DASJON010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.268"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(75..299)
                     /locus_tag="U3989_002751"
     CDS             complement(75..299)
                     /locus_tag="U3989_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716161.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(319..741)
                     /locus_tag="U3989_002752"
     CDS             complement(319..741)
                     /locus_tag="U3989_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1716162.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(883..1593)
                     /locus_tag="U3989_002753"
     CDS             complement(883..1593)
                     /locus_tag="U3989_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716163.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1611..2108)
                     /locus_tag="U3989_002754"
     CDS             complement(1611..2108)
                     /locus_tag="U3989_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1716164.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2145..>2584)
                     /locus_tag="U3989_002755"
     CDS             complement(2145..>2584)
                     /locus_tag="U3989_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1716165.1"
                     /translation="RDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPGIIQEK
                     LAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFIIRENNY
                     SNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT          656 a          524 c          423 g          981 t
ORIGIN      
        1 tcatgaacat tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat
       61 tctagtctca aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg
      121 atgaattctt ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc
      181 atttttcccc agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc
      241 tcactatgtt ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact
      301 gtttttcctc ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc
      361 ttcagggtca gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc
      421 ttccatgttc tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg
      481 tcctaatacc tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa
      541 ttcttttgag atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga
      601 aatttctgca ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata
      661 aagcatcaca cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc
      721 ctgtctataa actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat
      781 tcaagaaaaa taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa
      841 aaaaaaacgg caatgaatgc cgctcttctt atttcgattc ttttataatt ctgcttctga
      901 ttcatctttg gttagtgtgt gtctaacact agttacgcca aatgtttgat cgattttttc
      961 cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt tcagcattga tggattcaat
     1021 gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt cacgccagtt
     1081 gagacagcga cagatcgctc cttgttcatc atagtagatg atttcttccg gaagtgccgg
     1141 agcatcggaa tctgcaccta aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg
     1201 cagatttcct gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa aagacaagga
     1261 aatactatta tagatatcta caagcggatt gattgggaaa aattcccgtt cttgatttgc
     1321 acgtttcagt aaagcttcaa tagaagaacg tgcacctttt ttagtcttga atgtggtaaa
     1381 tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat gtttcttctg taaggtaatt
     1441 tcttgcttct tttgctgcct cattcaataa ttgatgatac gtttcttcac tttcttttgt
     1501 tacgtggtta tcgataccat ttacggataa gatttcgatg gttgcttcag gaaatacttc
     1561 ccagaaattg gtatccacta ttactttttt catctgttca cacctcttga ttagaataat
     1621 tgcttgatga atagatagtc tgtttgggga tcatctccca tataaaaaac atgttcgctg
     1681 tatttgataa atcctttttt acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca
     1741 ccgagccaaa tggctgtttt ttgcttttct tttgcttttt caatagctgt atctagtaaa
     1801 aatgtaccga taccttgatg tttaaatttt tttcgtacat agattcgctc gatttctaaa
     1861 aaatgatcag ccatttcctc agattgactc gttcctgtat ttagtttcag gtaacccgca
     1921 ggctcttgat cgatactcac taggaaaaaa tcggtttctt tatcttttag ctcttctgta
     1981 agtttttcta gtgcataagc ttgtgtcaag taatcagcta aatgctccgg tgaattatct
     2041 tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa
     2101 ggcgtcattt ttcgaatttc gtatttattc acggcttctt cctttcaata gatcctttta
     2161 ttgccttttt tgacaaaatt ccaatcttcg ctaacattct tgcatacttt atttaaaaga
     2221 tattccaatt gttttgcttc ttcgtcagaa agaccattta atgcgactgc attggagtag
     2281 ttgttttctc gaatgatgaa aggatagatt tctgctcctt ttttagtcgg atagagtttc
     2341 ttgatttttt tattttcttt atcttccaaa cgttcgatca tcccattgga ttctaatttc
     2401 ttgattgctc gggcagtcgt tgtacgatct actttgatca tttctgctag tttttcttgg
     2461 atgattccag gattttcgca aatacgtact aaataaagat actgtcctct tgttaaatcg
     2521 acttctttga attcgatatt gctaatagaa tctagcgctc gggcaatcat tccgatgtct
     2581 cgaa
//
LOCUS       DASJON010000262         2583 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.269, whole genome shotgun sequence.
ACCESSION   DASJON010000262 DASJON010000000
VERSION     DASJON010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.269"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..635
                     /locus_tag="U3989_002756"
     CDS             <1..635
                     /locus_tag="U3989_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN1716166.1"
                     /translation="FKLDSILIVMVALVVTALTGGLGIQGMLELLGTSFVNNRGMAIF
                     IIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAFNVSFGGVAGF
                     VRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFIGGAGALLVQS
                     TLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGKGQ"
     gene            637..1581
                     /locus_tag="U3989_002757"
     CDS             637..1581
                     /locus_tag="U3989_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN1716167.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1780..2424
                     /gene="pcp"
                     /locus_tag="U3989_002758"
     CDS             1780..2424
                     /gene="pcp"
                     /locus_tag="U3989_002758"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN1716168.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
BASE COUNT          768 a          456 c          601 g          758 t
ORIGIN      
        1 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
       61 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
      121 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
      181 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
      241 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
      301 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
      361 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
      421 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
      481 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
      541 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
      601 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
      661 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
      721 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
      781 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
      841 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
      901 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
      961 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
     1021 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
     1081 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
     1141 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
     1201 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
     1261 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
     1321 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
     1381 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
     1441 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
     1501 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
     1561 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
     1621 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
     1681 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
     1741 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
     1801 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
     1861 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
     1921 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
     1981 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
     2041 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
     2101 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
     2161 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
     2221 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
     2281 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
     2341 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
     2401 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
     2461 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
     2521 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
     2581 caa
//
LOCUS       DASJON010000263         2568 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.270, whole genome shotgun sequence.
ACCESSION   DASJON010000263 DASJON010000000
VERSION     DASJON010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.270"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(242..391)
                     /locus_tag="U3989_002759"
     CDS             complement(242..391)
                     /locus_tag="U3989_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716169.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="U3989_002760"
     CDS             853..1065
                     /locus_tag="U3989_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716170.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..>2568)
                     /locus_tag="U3989_002761"
     CDS             complement(1343..>2568)
                     /locus_tag="U3989_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1716171.1"
                     /translation="PEMILRDRPFRAAIRESWSLTKSRLLAITGQFIVIGGTILLLSS
                     LGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNIVMSTVGIFYIIVDFMDDE
                     GFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCLYNMDYLTSAVQTKPVTVS
                     HRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGHFVVMHDANLRHLTGVNGT
                     PQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKLLIEIKTSKKDSDDLVERF
                     VHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILPFNIVGPPVTPADFLTMEY
                     STINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGIITDEMGLLNESVKQTDEE
                     ITYSDKLLNFVLGVG"
BASE COUNT          874 a          449 c          454 g          791 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggta
//
LOCUS       DASJON010000264         2553 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJON010000264 DASJON010000000
VERSION     DASJON010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.271"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..135
                     /locus_tag="U3989_002762"
     CDS             <1..135
                     /locus_tag="U3989_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1716172.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            360..2222
                     /locus_tag="U3989_002763"
     CDS             360..2222
                     /locus_tag="U3989_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1716173.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2222..>2553
                     /locus_tag="U3989_002764"
     CDS             2222..>2553
                     /locus_tag="U3989_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1716174.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          922 a          383 c          485 g          763 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat ttaaaaaatt tgaaaaagcg tttgattatt
      181 tatattacga cttagtgcaa taaaaaacgc tttttaacag tacatgtagt cgtctttata
      241 atagataaac tctgtaatat tagttaaaaa gctataagtt gcacttagtt aagtgcaaac
      301 atttcatatt gtcgttagtt tagaaaacgc ttatattagc aatgtaagga gagatgataa
      361 tgcagcagct aacaaaaaga gaaaggcagc ttttgattta cttggtggaa atgtctgaac
      421 cggtaacaat caaaaagttg gctgaaaaag aaaatgttag tgttcggacg ataaaatacg
      481 atctagatga tataagagag tggctgaatg agcggaagca agtcctatat agcaaacgta
      541 gtcagggaat ttggatgaat attagtagct ctgatcgaat caaattaaaa agcgaattga
      601 tggatgttaa tcgttttgaa atatttgcag atcaaagtct ccgaatcaaa cgtttactaa
      661 ttatgctttt agtaacaaaa gaagctatta ctggaatgaa aatggccaac gcattagatg
      721 tcagcaaaga tacgatcatg aatgatttag atgtgctgga aaataatttt cttaaggaag
      781 gattgacctt gaatcgtcag gcgcgaaagg gtttttggat cataggagaa gaacgtctag
      841 ttcgattaac gattgaagag attcttcaaa aagagttcac ggattatgac atttataaat
      901 taatgtcatt gttattgaat ggcggtgaga ctgaatactt tgaaatgtat tctgctactg
      961 ctacacctat ccaagaggta tttaatcaag taatcattcg aatgcgacac ttactggaat
     1021 ttgaaaatct agagaaactg aattacgcgg aattactaaa tattctgatt cgagtgacaa
     1081 ttgctaccgt tcgcttacgc aaagaggcta cgattggtcg ttatcaattg gttgctgaac
     1141 aagaattaga caaaaacgat ctttcttatc gcgtcatgca gcaggtcttt gattattatc
     1201 agttgccact gttcgaggat gaatatcatt atatctatag tgatacattc gacacgattc
     1261 accagcaaga cgtcatgaaa ctagttgaga atgtgattta taaagtaagt aaagcaatca
     1321 accattcttt ttatcaagat ccacaattgt tgacgaatct gtatgctcat ttatccatgc
     1381 gattatcgag aaagaaaaaa tttgtcaatg aatataatcc ttttaaagaa gatatcaagc
     1441 agaaataccc ggaattattt gatgcgatca agcaagcagt tcgacaagaa ataagtgaac
     1501 aaaatattct gatcaatgat tctttcgtgg catatattgc cttacacttt ttggtttcct
     1561 acgaaagaga agaagatcta cgaagcgtta gagccgtcta catttgttca acagggttgg
     1621 gtgtgactag tctgatcaag caaaagatca atgaagaatt aaataatatc actatcgtgg
     1681 catttgcttc agtattgaat gcgcaagaaa tgattgctac caaagatcct gatttggtga
     1741 ttagtatttt tccaattaat ggcatcgatc gtcctttcat caaagtccat ccattaccca
     1801 ctgaacagga cttgaaaaaa attcaacaaa taataaaaaa aatacttgct tcttccaatc
     1861 agcaggaaag gcataacttg aatcttagag aaacgtccat caataaagat actttagaag
     1921 actttagcaa ggaattgatt ttgaaagctt atacgattta tgaacgttta cttgtcattt
     1981 tggaagcaga attgatccaa gaatatcgag aagctttttt gcttcatgta ttcttaatgg
     2041 ttcatcgtat cacatttgat caacaatata tggcagaagg tcgtcaagta gaccagatta
     2101 ttttagacaa aggaacactg gtaggaaaaa tcgaagcagt ttttgcagaa aataatttag
     2161 gaatcaatca gtcagaaatc agtgcattat tctcatatat aaaagaagga gcgtcaagtt
     2221 agtgaaaatg tcagacgagg ctttaaaaat tatcgaggaa agtcaaaatc gggaaaagtt
     2281 aaaacaagtg attgatctaa ttgaagagcg attagtagaa catgctattg taccaaccga
     2341 gttgcagtgg accattctaa ttaatcactt gaatgagatg ctgaaacgtt ctcaaagaaa
     2401 ggaaacgatt cccgtagtcg atcgggattt gttcaaagaa gtatccaaag aagccataac
     2461 tatttctgat gaaatagtta gaagtatcgg taatttaacc gatgatgaaa tatatgtttt
     2521 gtcgattcat tttgaaacag caaaaaataa tta
//
LOCUS       DASJON010000265         2547 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJON010000265 DASJON010000000
VERSION     DASJON010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.272"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(262..1872)
                     /locus_tag="U3989_002765"
     CDS             complement(262..1872)
                     /locus_tag="U3989_002765"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN1716175.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT          780 a          529 c          397 g          841 t
ORIGIN      
        1 aaatttgtta agacaattct taaccaacga ccctatttta ccaaacattt cggtagtttt
       61 ctactaaaat caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa taaaatatcc
      121 aatagcgctt taatcgaaaa aagtcttttg ctttctatct tttttcctac aaataataac
      181 aaatcagaaa aagagaaaag tgacaagaaa attgcttatc cccttctctt gctacttttc
      241 tctttctatc aagttcaaaa attattttgt ttcgtgatct gctaatgatg ctccgacaaa
      301 tgctttgatt aatttttgcg ggcgatttgg acgagaaatc aattctgggt ggaattggca
      361 tcccacaaag aattttttct ctggcagctc tacgatttct actaaacggt tatctggtga
      421 gacacctgaa aagactagcc cttgttcttc aaacaattga cgatatttgt tattgaactc
      481 atagcggtga cggtgacgtt cttgcacaac atcggctcca tcatatgctt cagccgtctt
      541 cgttcctttt ttgattttac atgggtataa tccaaggcgt aaagttccac ccaaattctc
      601 gatgttttcc tgatcagcca ttaaatcaat aatattgttt gctgtatctg gattcgtttc
      661 agctgatgac gcatcttcta atccaacgac gttacgtgca aattcgacac aagccatctg
      721 cataccaaga caaataccta aaaacggaac gtcattttcg cgagcataac gaatcgcttc
      781 gattttccct tctactccgc gatcaccaaa tccaccagga accaagattc catctgcgtc
      841 tttcaaacgt tcttctacat tttctctcgt taattcttgt gctttgatcc aatcgatctc
      901 gatgtctgaa ttataggcga aaccagaatg tttcaatgct tcaactactg agatatatgc
      961 atctggtaac tcgacatatt taccgacaag cgcaatccgt acttttttct tcaagttcaa
     1021 gacacgttct tctaattctt tccattctgt catgtctgct tctggtgtat ctagttttaa
     1081 atggtcgcaa acaatttgat ccattccttg tgcttgtaaa actaatggga tggagtaaag
     1141 tgtatctaca tctggcgact cgataactgc ttctggttcg acatcacaaa attgtgccaa
     1201 tttgtttttc acgctttgag aaacaggttg ctctgtacgt accaccaaaa tattcggttg
     1261 gatacctaaa ccacgtagct cttttacact gtgctgcgtt ggtttagttt tcatttctcc
     1321 agcagctttc aagtaaggaa tcaatgttgt atggatatac ataacattat ctgtaccgac
     1381 atctgctttc atttgacgaa gtgcttcaag gaatggcaaa gattctatat cccctactgt
     1441 tccccctact tctgtaatga tcacatctga atcagtcatt ttagctgctc gcatgatttt
     1501 ttctttgatc tcatttgtaa tatgaggaat aacttgaaca gttgctccca agtattcgcc
     1561 cttacgttct ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt tggagtattt
     1621 gttcaaattg atgtcaataa aacgttcgta gtggcccaga tccaaatctg tttcagcacc
     1681 atcatctgtt acgaatactt ctccgtgctg atatgggctc atcgtccccg gatccacgtt
     1741 gatgtatgga tcaaattttt ggatagttac ttttaagccg cggtttttca ataagcgtcc
     1801 taaagaagct gcaacaatcc cttttccgat tgatgaaacg acaccgccgg tcacaaaaat
     1861 atattttgtc ataagttcaa aaactccttt ttaatttatg tgcaggaaaa gtctcagaca
     1921 ataaacaaag ctccctattc ttcaataaga atagggagcc gaaatttctg taaacttctg
     1981 accctctaaa agggtgccca aggtgtataa tacaagctga ctaagggaaa gtcaagtact
     2041 caaatgaata ctatccgctt acaatttttt ctttttcgat atcctttgag aaattatttc
     2101 ttaagtcctt tatgagtgca aaaaaaattt aacccacata ataagcttga tataaacata
     2161 gctaacgtta aacctaagca aatcagaaaa tcgttactga aaaattttaa gtgaaataat
     2221 agataaaaaa ataaaacaaa cattatgata gataatatcc cacctaacaa agaactactt
     2281 tttcctactt gcagcaacag tttatatagc ttatcgtaga aacaatcttt ttctttcacc
     2341 cttatttacc tcctttacgt aaaaaacgta gtccacagtt ctcggatttt tggcatatct
     2401 tttctattaa ataatatcaa aaacaaaaca attccactaa ttttttcaaa gatgaccttt
     2461 ttttcaacaa aagtgccttg tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa
     2521 taaggagatg cgacaaaagt cgtgcct
//
LOCUS       DASJON010000266         2547 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJON010000266 DASJON010000000
VERSION     DASJON010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.273"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            221..>2547
                     /gene="esp"
                     /locus_tag="U3989_002766"
     CDS             221..>2547
                     /gene="esp"
                     /locus_tag="U3989_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN1716176.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          923 a          393 c          509 g          722 t
ORIGIN      
        1 catatctttt tatatgtttt cagctgctga atggttaaaa agcacaaata tccgaaaaaa
       61 acatgaagaa atgtactttt tttgttgtaa tgttcatgaa attctctaag taaattagtt
      121 aacataaggt tagtaagatt cctattctgt gaataatacg tatcagattt atcttttatt
      181 cttactagga aagtaattat taagggagtt gatttgaata atggttagca agaataataa
      241 gagagtattt ctagaaaaaa caaagaaacg agtattgaag tacagtatca aaaaattaag
      301 tgttggggtt gcatctgttt tgattggtgt tggtcttgtc ttgggaacaa ctgaactagt
      361 taaagcacaa gatgaaattt ctccaagcag cccacttgaa acagcgataa gctccgtaca
      421 agtaggtgac aaagtagctt cggggaatac ttttcaggag aatcctggtt atacgaaaaa
      481 ttataacttt tcggatttac aatttagtcc tcaagaacta actggagaca cactaaaagg
      541 caatacaatt ggatttgaag tttacggaaa acataatatt gcagcatcaa ctaaaaactg
      601 ggaaatccgt cttcaattag atgaacgatt agctaaatat gtagaaaaaa ttcaggtcga
      661 tccaaagaaa ggaattggat cttctagacg gacctttgta agaattaatg attcgctggg
      721 tagacctaca aatatttgga aggtcaatta tattcgtgca agtgatggat tatttgctgg
      781 ggcagaaaca accgatacgc aaaccgctcc taatggggta attacgtttg aaaagagcct
      841 agatgaaatt tttaaagaga taggcataga caatctcaag accgatcgtt taatgtatcg
      901 aatctacttg gtaagtcatc aagacgatga taaaattgta ccgggtattg atagtacggg
      961 ctacttttta acagattcag atgatttcta taatagctta gatgtatctg aaaataatcc
     1021 tgaccagttt aaacatggat cagtaagtgc aaaatatgag aaacctaata ctcaaacaaa
     1081 agatggttca ggatccacag gtgctaatgg agctataatc ttagatcata agttaacgaa
     1141 aaattataat ttctcatatt catcttccgc aaagggcacg ccttggtatg ctaattacaa
     1201 aattgatgaa cgattagttc catatgtagc gggtatacag atgcacatgg ttcaggctga
     1261 caaagtaaca tatgatgttt cgtttgaatc cggaacaaaa gtagctgatt tagcaatcga
     1321 gcgacgggaa ggtcatgaaa attatggcat aggttcaata actaataatg atttaactaa
     1381 acttatcgat tttgctaatg caagtccacg tccagtcgtt attcgatatg ttttacaatt
     1441 gactaagcca ttagatgaaa tcttagaaga tatgaaagca acagcacaag ttgaagaaaa
     1501 taaaccattt ggtgaagatt tcatctttga ttcttggttg tcggatacga ataaaaaatt
     1561 aatccagaac acttatggaa caggttatta ttatttgcaa gatattgatg gtgatggaaa
     1621 ccctgacgat aaagaagaga gcggagacac gaatccatat atcgggaaac ctgaattaga
     1681 agaagtatat gatgttgaca caacagttaa ggggaaagta ttcatccacg agttagcggg
     1741 aacaggtcac aaagcccaac ttgttgataa agaaggtact gtattagcag aaaaaactat
     1801 cgctccaaat gaaaaagatg gggctccaat ttcagatact gtagaatttg aatttacggg
     1861 tgtagattca agtaaattaa tcgcgaaaga tgaattaaaa atccaaatcg tttctccagg
     1921 ttttgataaa ccagaagaag gttcaaccgt tattaaggaa tcaccaaaag cggttgataa
     1981 acaaaccgtg gtagttggat ttaaaccaga tgctaaagaa tcaattcgga ataataaaaa
     2041 cttacctgaa gatgcagagt attcatggaa aacagagcct gatacttcta acgttactga
     2101 tagtacgaaa ggtattgtaa ctgttaagat cggaaatcga actttcgacg tggatgtaga
     2161 gtttgctgta aaagcttctc aagctatgga aaatgatgca acatacgtac ctataacaac
     2221 aactccagaa acgacagttc aaagtggaaa acctacattt gataaaccag atgttccttt
     2281 agctaaagat gccttttcaa tttcagatgt ctataataag tacttcggta atgcaagtgt
     2341 tgatgcaaac actggtattg ttacattcac tccagctaaa ggtgtaggag aatcggagcc
     2401 gattactgga acaattccta ttaaaattgt ttaccaagat ggttctgtag gcacgaccga
     2461 tttagcagta actgtaagta aagatattta tgaaaatcca ggagaaaaca ttcctgcagg
     2521 ctaccacaaa gtaaccttca ccgcagg
//
LOCUS       DASJON010000267         2519 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.274, whole genome shotgun sequence.
ACCESSION   DASJON010000267 DASJON010000000
VERSION     DASJON010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.274"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..758
                     /locus_tag="U3989_002767"
     CDS             <1..758
                     /locus_tag="U3989_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1716177.1"
                     /translation="PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEG
                     IKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKP
                     QVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVT
                     ATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKT
                     GESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            892..2235
                     /locus_tag="U3989_002768"
     CDS             892..2235
                     /locus_tag="U3989_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1716178.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2347..>2519
                     /locus_tag="U3989_002769"
     CDS             2347..>2519
                     /locus_tag="U3989_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1716179.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1000 a          325 c          456 g          738 t
ORIGIN      
        1 atccagatgg atcatctgat gaagttgaag tagacgttac agtgacagat actcgttccg
       61 atgcagataa atataatcct gaaggacaaa aagtgactac agacttaaat aaagagccag
      121 atgcatctga gggaattaag aacaaagaag atctaccaaa aggaactact tatacttgga
      181 aagaaaaagt agatgttagt acagctggaa acaaaaaagg tactgtagta gtaacatatc
      241 cagatggatc taaagaagaa gtagaagtta ctatttctgt agtagataaa aaagcaccga
      301 ataaacctca agttgatcct attacagagg gtgaccaaat tgttactggt aaaactgaac
      361 caaatgcaga ggtgacagtt acattaccag acggaagtca acaccatggg acagctgata
      421 aaaatggtaa ttttacagtt aaagttccta aattagaggc agggacaaaa gtgatagtaa
      481 ctgcaactga tgaatctggt aatactagtg aacctactaa tgtagttgtt tcttccaaca
      541 aaaaagacag tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca
      601 gcaatcaaga caaaaataga ggcaaaagcc agtcctcaaa agtttttcct aagacgggag
      661 agagtgacag taatatcttt actattagcg gtggtttaat actgttagga acgttaggat
      721 tattagggta taaaaatcga aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc
      781 tctgagaaga ggaagaatct ttttcttcct ctttttgctt ttttatgatg ggaaataagt
      841 gttagttaaa gatgaatata ctttttatag aaaggaaaag aatgtaatta agtggaaaaa
      901 atagtaatta ttggttcaaa ccattcaggg ctagccgcag ctcgatttct aaacttaagc
      961 gattcaaatt atgaagttat tctaatcgat aaaaaatcag aaattagtta cttggaatgt
     1021 gggacatcgt tatttataaa aggagaagta agtaatttag aagatctttt ttatcaaaat
     1081 agaatgacac taaattctga gataaaaaag ttatttctag agacagaagt tgacaaaatt
     1141 gatattcaaa ataaaaagat tagttgtgta aaaaaagatg gtacaagatt ctctcaagaa
     1201 tatgacaaag ttatattagc tacaggttca aagcaaatta tgctagatat accggggaat
     1261 aatttagcta atattttttc gataaagaat tttccaaatg caaaagagat ttttaaagct
     1321 ttggatgata gtactattaa aaaagtagga attattggag caggatatat tggagttgaa
     1381 attgctgaag ccataagaaa aagagggaaa gaagtatatc ttttcgatgt tgcagataga
     1441 gtgttgtcga cttattacga ccgtagtttt tcagataaag ttgaagaaat actatcaaaa
     1501 aatggaatac atctttgtct atcagaaagt cctaaaaaat atttaggaag aaaaaagatt
     1561 gaaaaaatag tcactacaag tggtacccaa tactctatgg atatggtcgt acaagctata
     1621 ggatttctat ctaatagtcc aattggggaa aaggaactct tgagagatgt tagtggagca
     1681 tatctaacta atgaatacca gcaaacaaat ataaaagata tatatgctat tggggattgt
     1741 gctacaactt tttttacgtc cattaataga agtagtgttg atttttctat ttctaatgct
     1801 ctaagaacag cttatattgc aactcaacat attaacaatc aagattttac accttctggt
     1861 tcccaattta caaatggctt ttctatattt aattacaatt tttacgcagc aggtttgaat
     1921 ttaaaaacag cacgattatt aagtgagtct atagattata tagaaataga agatttcatt
     1981 agacctgctt ttttgagaaa aaatagtcgt gtaaaattaa gaattagctt tgaaaaaaat
     2041 acaaagagaa tagtaggtgt acaactttgt agtcaagaag atttatctgg actattatca
     2101 atgttttctc ttgctataga agaacaaatt acattagaca aattgaaaat gttagattat
     2161 ttatttcatc ctagttttag ccaaccctat aattttattt taaaggcagt aacaggatta
     2221 aacattgaaa cataatcgtt actaaatgaa atcaagaaat atactgtatt actcaattca
     2281 ttgaaaatat ttatcataat ttttaataga aaacactatt aaagagaaaa aagagaggta
     2341 aatcaaatga aaaaatcttt aaaacttgca tcagcagtta tattaatgtc ccaactcctt
     2401 atagcaacac ctttccatgt gttggcaagc gaaaattcaa catacaaaat tgcaaaagaa
     2461 agaaccgaga cgactgaaat gagtaattcc gatgtaacag cagagagtag tgaactttt
//
LOCUS       DASJON010000268         2497 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.275, whole genome shotgun sequence.
ACCESSION   DASJON010000268 DASJON010000000
VERSION     DASJON010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.275"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1149
                     /locus_tag="U3989_002770"
                     /pseudo
     CDS             5..1149
                     /locus_tag="U3989_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="U3989_002771"
     CDS             1320..1778
                     /locus_tag="U3989_002771"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN1716180.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="U3989_002772"
     CDS             1937..2407
                     /locus_tag="U3989_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN1716181.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          830 a          475 c          469 g          723 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctttg
     2461 cgtggcgacg tcctactctc acaaggggca acccctc
//
LOCUS       DASJON010000269         2488 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJON010000269 DASJON010000000
VERSION     DASJON010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.276"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(258..554)
                     /locus_tag="U3989_002773"
     CDS             complement(258..554)
                     /locus_tag="U3989_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN1716182.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            909..1265
                     /locus_tag="U3989_002774"
     CDS             909..1265
                     /locus_tag="U3989_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1716183.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1275..2174
                     /locus_tag="U3989_002775"
     CDS             1275..2174
                     /locus_tag="U3989_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN1716184.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          818 a          425 c          465 g          780 t
ORIGIN      
        1 gaaaatatga gaagctattt tctgtccagc gttcttatta ctcaaaacaa atcgcagttg
       61 tcacaatctc ttctaaacga tgttccatct gctgcttctc ggcaataagc ccactagact
      121 agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt
      181 gtcacaatct cttctaaaca atgttcaatc agctgacctt cggtattagc tcaaagcact
      241 gatttcacaa cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc
      301 atttttcgat aaaaatcggc gggaagctta ctccaccgtt ttagtaattg atagaaataa
      361 ctctcttctg ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt
      421 gaagctaatt ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt
      481 tttacttcaa tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt
      541 atatattcct tcatagtgat tcctccaaag aagtctttta tttgataagt ttactctacc
      601 gaagagttgt taataatccg tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt
      661 tttttatttt ttatagtatt ttttgttatt taagaaagga ataaggttaa acaaataaca
      721 ggttgctttt tgggtctttt tccgttcaag aaactgaacg aagaaaaggg aaagttgtct
      781 cttttgtgat aagagaaatc ataataaacg aatgacttac tttccaataa attgagaaca
      841 gtcagaacca gacaaattag ttgcatctga tgaataaagt gattattcta tgacatgagg
      901 tgatacagat gaaattagaa gtgacaaaag aagcacaaga agtattgaag aataaattag
      961 aagcagacga tcaactttta ttagatatcg aaaacggtga cggtccattt gcagaaagcc
     1021 aagtcagttg ccaattggat acggcatttc gattgatcat tgtcaaaaaa gatacacaaa
     1081 cagatctaag tctttattct gtagtggctg atacaccagt tggaccaatc aaaatcaaag
     1141 acagtgcaat actttacatg gatgatccat ccacacttgc tttagaacca acttataata
     1201 gtttacaatt aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag
     1261 actagaggga taaaatggga aaattggcga tcatcagcga cctacatgtg gacatcaata
     1321 aattcggcga atcagagctg atgcagcttt ggcaagtttt gcaaaagcac aagatcactc
     1381 ggcttcatct agccggagat acggctaata aagtagaccg ttgcctagct gttgtcgaat
     1441 ttttcgtcaa taaaggatta tctacgacat ttcatttcgg caatcacgag ttggcagatg
     1501 tgcccaatga acagatgatg gaccattttc cagacccaca ttttttaaat gaacggtata
     1561 ttgctttaaa tgagcagacc gttcttttag ggatgaatgg ctggtacgac tatcagtttt
     1621 ccgaattgaa agattacgag gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt
     1681 tcatccagcg tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa
     1741 atttgctaga ccagctggaa gcaaaaaaac ttgaggtgat tttatccact catttcgttc
     1801 ctaaacgggc atttatcatg tatcaatctg ctccttatga gcgttggaac aaattaaatg
     1861 catttttagg atcagaatca ttcggagaat tacttgaccg ttattcaaat atcaaacaag
     1921 tagtttttgg ccatacgcat cgtcgctttg aagataaatc catcggtcac acgatttaca
     1981 gctgcagacc ttttggttat tattatgaat ggcagctgac ccgagatttt gtcttgaaag
     2041 aacagttgct agacagctat gatcctatga aattacgtgt cctcttgcgt gcccatcacc
     2101 cagcttttca ggcctatcaa acacaacatt tgcaagagga atttgaacaa gcgatgacaa
     2161 tcgttccgta ttgagacagc attagtaatg tgtaaaagaa aataccttgt aaaacataag
     2221 gataagtaag agcagtagat ccatttatcg tggatatgct gctttttttg cggaaaataa
     2281 tcagcgaatg tttatttgat cggatgattt gtaactcgca gagtactaga tgcaagcgtg
     2341 tattcttttt ttgaacgata atcacaaaaa aaaatataat atacattttt tatttaatta
     2401 taattaatat tgattgaagt ctaggcagtc aagtcgagag tgtaaaatat tttgtgtaaa
     2461 tgaaaaaatc catacaaaaa aggaagtc
//
LOCUS       DASJON010000270         2448 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJON010000270 DASJON010000000
VERSION     DASJON010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.277"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..179
                     /locus_tag="U3989_002776"
     CDS             <1..179
                     /locus_tag="U3989_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1716185.1"
                     /translation="ILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEI
                     FLSYVQEAFYSSLI"
     gene            complement(1045..1575)
                     /locus_tag="U3989_002777"
     CDS             complement(1045..1575)
                     /locus_tag="U3989_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN1716186.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1572..2189)
                     /locus_tag="U3989_002778"
     CDS             complement(1572..2189)
                     /locus_tag="U3989_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1716187.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          806 a          465 c          273 g          904 t
ORIGIN      
        1 ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg aatcagacat
       61 ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg aattacagaa
      121 caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc ttaatttgac
      181 aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt acctttctcc
      241 tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata ttcatcaaac
      301 tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt aatcttcttg
      361 ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga ttcttttctg
      421 ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact agtcctttgt
      481 tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac tccaagactt
      541 ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta ataaatatac
      601 attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct ttacaatcct
      661 aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct ttcttgatag
      721 tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt gctcaaaagt
      781 ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt tagggtcttg
      841 tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta tttggtttca
      901 ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg gagccccccc
      961 tataatctca ttattatttt catgactttt tactaccgtg gaattacaag caacaacaat
     1021 attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc aacaatgttt
     1081 acctataact attttattat ttttattcaa aatcctatga gtattcaatt caaaaatcgt
     1141 atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat ctttgaattg
     1201 aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg acccaatatc
     1261 taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg ctggcccttg
     1321 tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag tatttaatac
     1381 tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt tccctctcga
     1441 tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg catctgaaat
     1501 atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat tctttattat
     1561 caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt ataaaggatt
     1621 acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa cgttcctagc
     1681 ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat ttttcctcca
     1741 tatactattg aggaccatat tttgggtttc tttatatttt catatttaat agacattacc
     1801 atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga taactctttt
     1861 gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc agataaagga
     1921 tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt atagaatatc
     1981 aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag ttccattagt
     2041 atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa atagaccagt
     2101 cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata taatatatct
     2161 gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag tatacactta
     2221 tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta atattcaatt
     2281 tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt tttatacgat
     2341 tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt tttcatccac
     2401 ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgtt
//
LOCUS       DASJON010000271         2414 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJON010000271 DASJON010000000
VERSION     DASJON010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.278"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..462
                     /locus_tag="U3989_002779"
     CDS             <1..462
                     /locus_tag="U3989_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN1716188.1"
                     /translation="VTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEVKTYELTD
                     EDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMIKEAKIFG
                     DFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY"
     gene            complement(507..2351)
                     /locus_tag="U3989_002780"
     CDS             complement(507..2351)
                     /locus_tag="U3989_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716189.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          810 a          389 c          448 g          767 t
ORIGIN      
        1 gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt
       61 cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac
      121 gaattgactg atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat
      181 tgggattgga attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct
      241 atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc
      301 tttggcgatt tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag
      361 aaatatgata aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt
      421 ggtgcgatag aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa
      481 tccacatata tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga
      541 acagtgatcc gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc
      601 gctacgtctt tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg
      661 cctcgactaa ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt
      721 tcctcttgtt cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga
      781 gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg
      841 ataggaagtt cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct
      901 aacttctggt aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc
      961 atttcgaaat tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg
     1021 tccgtatgca ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta
     1081 tttacttctt ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg
     1141 atttttaaga aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga
     1201 ttgtagctga taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt
     1261 gtttgataga tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag
     1321 acgatccaag gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat
     1381 gcaagtgaag tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca
     1441 ggaagttctg caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg
     1501 aacgtacaaa aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag
     1561 atgatgaaag ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt
     1621 tcaaaaagaa taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca
     1681 aagcaactta agagaataag tgagtattca agacgttttt gtttatacat caagtagatc
     1741 gtatagaaaa aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc
     1801 actgattgtg aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt
     1861 ggcagtccag catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata
     1921 taatttgctg gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa
     1981 tctttccgtt ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct
     2041 ttgattagta aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt
     2101 tcgtctttat ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga
     2161 tcatcttttg tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca
     2221 ggagaaaaaa taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata
     2281 atgattatgc tccactttat acgtaaataa aaagattctt ttttattata tatgttatta
     2341 aaaaaagaca taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa
     2401 actaacgtca atta
//
LOCUS       DASJON010000272         2395 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJON010000272 DASJON010000000
VERSION     DASJON010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.279"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            316..570
                     /locus_tag="U3989_002781"
     CDS             316..570
                     /locus_tag="U3989_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1716190.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="U3989_002782"
     CDS             571..888
                     /locus_tag="U3989_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1716191.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="U3989_002783"
     CDS             1447..2382
                     /locus_tag="U3989_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716192.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DASJON010000273         2341 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJON010000273 DASJON010000000
VERSION     DASJON010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.280"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1037)
                     /gene="brxC"
                     /locus_tag="U3989_002784"
     CDS             complement(<1..1037)
                     /gene="brxC"
                     /locus_tag="U3989_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN1716193.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERIL"
     gene            complement(1062..1637)
                     /locus_tag="U3989_002785"
     CDS             complement(1062..1637)
                     /locus_tag="U3989_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HEN1716194.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(1637..2236)
                     /locus_tag="U3989_002786"
     CDS             complement(1637..2236)
                     /locus_tag="U3989_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HEN1716195.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT          717 a          478 c          288 g          858 t
ORIGIN      
        1 ttcaaaatac gttctttaat tacttcatcg gcatttgctg aagataaact cacagttgtg
       61 gcaaaacgat ccttgatctt agaaaaatca ttatcagaat tgttatcaaa gtttgttacg
      121 tgatcaatcg cttcctgact tgtcactaat acccaagcct taccttcaca aattcgaccc
      181 aaatcttcca caacggtttg taactgcaaa gtcaactcac gattaccgcc gatatattgt
      241 ccgacctcat cgactagaaa ggcaatatga tggtctgctt ctttggaatc aagatagctt
      301 ttaactagat ttgcaaagtc atcaatgctt attaaataat ttcctgatgc tttttctaaa
      361 aaattatcaa tagcggcttc actcataaaa tcgattttaa gtgccgcttc tttgatatca
      421 tcttggatcc aatcaaactc atggcgctct tcttgccatt ttgccccatt aatctcttca
      481 aaagcagctt tgaatgcatc aaacttttct tctttaatta accgacgttc aaaatctgct
      541 agatgtggaa tcgtgcttaa tccttgcatt tcgttaaata cctttaaaaa aacattcacg
      601 attccgtttt tgtctttttt accacctgca tcactttttg aatcaatatt aaacagaata
      661 acatctgtat ttatttgtga gcaccgttcc atcgttgcca aagtcagtgg atctttgatt
      721 ttcccatctt ctttgaagta atcaatggct ttcttgtcat ttatttctcg atttgcgagc
      781 aagtaagaaa taatttttaa gaaatgagat ttacctgaac caaagaaccc ctgaatccaa
      841 acacccattt tatctgttgg tgtattgata ctccgttggt aggcatcaaa aattgtttta
      901 aagtgttttt gtagttcttt cgtaaccacg tattcttcta attcttgttg aacattttca
      961 tcctttgctt gaccgacctt aataactcct tgaatatttc ggtcaattgg ttttgcaaaa
     1021 atatctttaa ttagcattta atctcctacc ttccatccca actaaattct aaatgctcga
     1081 taataattat catctttatt ggtattaaga attgtcaaac ttaattcatc atattcccct
     1141 ggatacatca tcacaactgg tacgcgatca aatacctgat gcattttatt taaaatagag
     1201 tgcgctctaa ttaatggata taattcgcca attcctgtca ggaacacaac tgatttttct
     1261 ggtgtatggg catccacata ttcaaaaaat acattttctt cctcaggtaa atccattttc
     1321 aataaacgac caatgctttc cactaatttt tgtaaaccac gtcgttcttc catcttaatc
     1381 gctttttcag tgaaaccgcg tttttctaga tactctaaca taatcacata gagattataa
     1441 actttcactt cataattatc accaacagat gttccagata tatcattaat ataatctctt
     1501 acaattaact cgtctttcgg atcgtagtca aagatataat agcctacttc attacctaaa
     1561 ccacgattca ataaaaaatc ttctttttga attacttcat tcaacttttc caaatgctct
     1621 tgaatcgata atgacattat aaaattcctc ccagtgctag tacaatatct ttacgattct
     1681 tgtcaactaa ccattgaatc aatcgtttat ctaataatat cggagtcatt cgtccatatt
     1741 ctttttcccc tctgactaat cctgtttcaa ttagattcgt ttgataagcg gcaataagcc
     1801 gtttaatcgt ataatctttc cattttgaaa tatcttctga acttctccgc ttctctgtaa
     1861 aaaaagaacg gtaatcaact ggtgttaatt gcaaatttcc tttacgtaga ttttcagcat
     1921 atacctcagt cataaattca ttaaaaagat catcaatcat aaggatggaa atcaaattca
     1981 ctaacttttg attatctata tttaactgat tgaaaagttg caatccagct tcatccagtt
     2041 gactaactct ccttttcacg ttctgataga tttctttttg acggttactg ctcactgcaa
     2101 aaaaaatatt ttcatgttca tttttctctt taatttctat atctgtatat ccttctagtc
     2161 gtaattgaat atataacttg aactctgaaa accaaaagcg tttagaaact agaccagcag
     2221 aatatttttt attcaataga ttgcctccta atcatctata attcaataag taaacaagtg
     2281 ttttttgctc atcgttagca ttcatttttt agtatctact atacaataca tatttattat
     2341 a
//
LOCUS       DASJON010000274         2275 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.281, whole genome shotgun sequence.
ACCESSION   DASJON010000274 DASJON010000000
VERSION     DASJON010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.281"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            111..1724
                     /locus_tag="U3989_002787"
     CDS             111..1724
                     /locus_tag="U3989_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1716196.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          711 a          404 c          445 g          715 t
ORIGIN      
        1 atttctctat attttctgtc tattcaaaat aaacaataat tttccggcac aattatttgc
       61 tgttttttac cataacatag tacgatcaac gtagaaaagg agatgttttt atgaaagttg
      121 ttgtcattgg cggtgtagct ggcggtcctt catttgccac tcgtttccgt cgattgaatg
      181 aagcgcacga aatcattatc tatgaacgcg gagaaaatat ttcttatgca agttgtgctt
      241 tgccttatta tttaggaggt gtgatcactg accgtgactc gctgatcgaa cgtacaccag
      301 aaatattgaa aacaaaaaac aacatcgatg tctttactaa acacgaagta acagcaatcg
      361 atccttctac taagcgatta acagttaaag acctatccac aaatgaagaa ataaaaacag
      421 attacgataa gttgatcatc tcttctggtg ctagaccaga ttatccggat attcccggag
      481 tttttgaagc agaaaacggc tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat
      541 cgttccttga agaaaaaaat ccacaacatg tcgtcattct tggtgcaggt gttatgggtc
      601 tggaattagc tgagaatttc aagcatcgcg gcttagacgt gactttaatc gatcaattgc
      661 cacaagtcgc tttcccttat gatccagaaa ttgctaattt agtttatgac aaattgctga
      721 aagaaggatt agccgttcat ttagaaacaa gagttactga gatccgtgat aaaggtcgag
      781 aaatagtatt atcagatggc tccgtccttt ctgctgatat gctaattttt gctgttggtg
      841 tttctccgaa gaatgaagtg gtgaaagcaa ccggcataca attatcagat acaggtcaga
      901 tcattgttga tgaccagtta caaaccaatc tgccggacat ctatgcgatt ggcgatatta
      961 tcgaaacgac tagtgtagtg actggtcagc cgatccaaag tatgctttcc agtgcggcca
     1021 atcgtcaagg acatatgctg gcagatattt taaatggtac gcctatgcgc tatcgcggat
     1081 atattggtgc aggtgtcgca aaaatctttg atcatacagc aagttatgcg ggaatgacag
     1141 aacatgaatt aaaagcatca ggcataacaa attataaaac tgtttttatc actccctttg
     1201 accatgccta tttctatcca gaagctacaa gattaaatct aaagctgatt tttgatgcag
     1261 atagcggtcg tattttaggt ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg
     1321 gagaactttc tgtagcaatc accggaaact tgacagtctt tgatttgcct gatttggagt
     1381 tgccttactc tccaccgtat tctactaccc gtgacccgtt gaatatagct ggttatgtcg
     1441 caatcaatca aatgacgaat atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg
     1501 atttgaaaga agcgttcttt ttagatatac gtgaacctaa taaagcacct tctggcagta
     1561 tctcggcaac aaaaaatatt ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa
     1621 aagataaaaa agtttacatt actttcagaa gaggattgaa tacttatact tctgcccgaa
     1681 tcttggcagg tttgggtatc aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg
     1741 ggtagccgcg gtctaggagt tatttttact tgaggagtaa aaatgaaaaa gtatataggg
     1801 ttgttggttg gtatgtatat agaatagaag ataaatgtga agaaattgtg cattatttct
     1861 tcacatttat taggttatag tttaagaaag tttaaacatt gcgcaattta attattttag
     1921 ctgtttcttt tcataaaaaa acatgttgtg actctgagac aaatcatagt ccatagtgat
     1981 gtctctagag taactacgcc taatattacg aggcatcata ttaatctatc agcactagta
     2041 cctaaactag catccctttc gaatattttt tcttatataa taaagtttgc tttcactcct
     2101 ctaatctcac caaatcgact ggcttgcgtt gtgtgatact aggaactgat tggtttagaa
     2161 cgacttttct attgtaatca tatttttcgc catcctcgtt gtcttctaat ccttcaatga
     2221 atgatcccta atgtgtgtat taaataattt aatttttttg ttcctattta tttaa
//
LOCUS       DASJON010000275         2271 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJON010000275 DASJON010000000
VERSION     DASJON010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.282"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(138..437)
                     /locus_tag="U3989_002788"
     CDS             complement(138..437)
                     /locus_tag="U3989_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1716197.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            788..1420
                     /locus_tag="U3989_002789"
     CDS             788..1420
                     /locus_tag="U3989_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1716198.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1543..2181
                     /locus_tag="U3989_002790"
     CDS             1543..2181
                     /locus_tag="U3989_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1716199.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          761 a          362 c          331 g          817 t
ORIGIN      
        1 ggataaaata caatataaag aaacatctgt ttctttattt agaataaaaa aatacagact
       61 ctgttcaaat aaatgaacct agcctgcatt atgagattga ctatactttt taattcattg
      121 agatgaataa ctcgttttta taaatgccca aaagtaatcg gtgccgaagc cattcctcga
      181 cctaattctt tttcaaattt tcctattgtt gatatctcca tataaaacgg tgatactggt
      241 ttagacaatt ctcccctgat tgctaacgga cgtaaactac gttccaaatt atttatgact
      301 ttaggataat actgaccttt ttctaagcct gtctttgcgt ctatcaaaag tatacgttcc
      361 ttttctttga tctctggatt taaaacgaga tcatataatt ttagtaccat gttttctatt
      421 tcttcacttt tagacatcac gatttcctcc caaaaatata tttcttccca ctacattcta
      481 taaacaaagt atacgctaca tctagatttt tttaaatcat tactggattg ttaaaacctt
      541 tcaatacact gttatggcaa cgatttttat tttaaaaaca ttctaaatta aatcagtaaa
      601 aatttttatt ctaagatttt tattagaatt acgcctaatt tgtgtctctc agggcaattc
      661 taagaacgtt tcaagagata gcgacactat cgttatcgaa aaaggcgaaa acctatctca
      721 ttttctgcta atgtaagttg gtttagcttt tgttaatatg actttatgaa aatggaggta
      781 attaaatatg atttttccta aagttaatga agcaagcttg aataaaggat ccttttttca
      841 tatctatgaa aacgaagatt ttgaactgta taatatcctt tcacaaaaag aggtcatcga
      901 aaaccaaata atctgccatc caattattta caaatctatt ttcaagtatg cattttttgc
      961 ttccaatgat tttcaaagaa tggagattac cgactgtata ttcgaaaact gtgatttttc
     1021 aggctgtata ttgacaaaaa gcatgttgca tcgcgtgaaa tttgtcaatt gtaaattaac
     1081 aggtactaaa ttgatggaaa gttatattgg gaacacgtta ttcgagaatt gtaaaatgga
     1141 ctatgtaaat ttatctggtt caaacataaa agaaagtacc tttgagcact ctgtcttatc
     1201 aagcgcagat tttgtagact gttccctcac taaaataatg ttttctacta atgatttaga
     1261 aaatgctagc ttttttgata ctgatttaaa aaatatcgat ttaagtagca actcctttga
     1321 aatgattgaa gtacgctcgg aacatctaaa aaattgtacg cttaatcagt tgcaagcact
     1381 tggttttgct agaaaatttt tacaaataaa agtagtataa tcaaaatgaa tatataagat
     1441 aaatttttag caatacaaat atccataaaa aaacattgcc tattttaact cttcatgtat
     1501 actgactcta tcttcaaaaa ctgaatagta ggagttatta tcatgaataa cattttagga
     1561 aaaaaaatag aacttaaaaa agggatattg ggcactattg tcgttgtact cggtttgtta
     1621 gggttgctat ttgcgccatc cattattagc attttttctt taactatcac tggatttata
     1681 gcatttacac ttgtttttgg taaaaaagat acgaaaaaaa tgttctcgaa acctgtcgct
     1741 cctgtcaaaa atatagccaa atattttttt atcaatgtag taatcagtgc cgcagtctcg
     1801 gtatttttgc aacaaatttt gaaatggggt ttaacaggca atccaataaa tgaggctttc
     1861 agcccgttat tatttattat tttgcctatc atgatacttg gagaagaatt gttctcagtg
     1921 tattttctcg ctattttttc tagtaagttc agcattcctg tcgctagttt cttgtcagca
     1981 atcatctttg gatttatcca ttacagtact tatgataatg gaaatatttt acacactgta
     2041 gcacacatcc ttttgataca aggggtggca agattgttat tcaaccaagc tgccattaaa
     2101 agcaactcga tcattacaag ttgggcagtt catgtgatat ttgatttcac agctatattg
     2161 ctagtcgttt tattctccta aatttttata gaaataatac atgctctatt gctaagaaag
     2221 catactttat ttcctttaaa caaaaagaca gatgtatcaa tggattaaaa a
//
LOCUS       DASJON010000276         2261 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.283, whole genome shotgun sequence.
ACCESSION   DASJON010000276 DASJON010000000
VERSION     DASJON010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.283"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..613
                     /locus_tag="U3989_002791"
     CDS             <1..613
                     /locus_tag="U3989_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN1716200.1"
                     /translation="FACDAGMGSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNT
                     LIVTQVELQERAKQKAPSARFVSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEE
                     FLSANIKQIKEILLVHDDRSGSATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSL
                     IVAKESLAEKVQMAAPQSIHLSVNSMVNPQKYESIAQYLKQTA"
     gene            628..1065
                     /locus_tag="U3989_002792"
     CDS             628..1065
                     /locus_tag="U3989_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1716201.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            1078..2229
                     /locus_tag="U3989_002793"
     CDS             1078..2229
                     /locus_tag="U3989_002793"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN1716202.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT          821 a          397 c          484 g          559 t
ORIGIN      
        1 ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct tgagaaaaaa
       61 agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc aactgacaga
      121 tgactcgaat acattgatcg ttactcaggt cgaactacaa gaaagagcaa aacaaaaagc
      181 accgagcgcg cgttttgtct cagtagaaaa ctttttgaat tcacctcgat atgatgaaat
      241 cattcaagaa ttaagcagca agcagaaaac atcagtttct gaaattgaga atgagccagc
      301 agaagagttt ctttcagcaa acatcaaaca aatcaaagag attctacttg tccacgatga
      361 tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa gatatattag acaagaacca
      421 tttaactatc ccgttaagaa aagtcaatat ccaagattta aaagatgaac ctgattcact
      481 gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg gctgcacctc aatccattca
      541 tttatcagtc aacagcatgg tcaatccgca aaaatacgaa tcgattgcac aatatttgaa
      601 acagacagct taagggagga aaacagcatg caaagtttgc cattcgacat gatcgagctc
      661 aatcgatctt ttaaaaccaa agaagaagcg atccgctatt gcggtcagat gcttgtagaa
      721 gcagggggcg ttgatgagga atatatcgat gccatggtag atagagacaa aatgttatct
      781 gtttatatgg ggaattttat cgcgattcct catggaacag atgaagcaaa aaaatatgtg
      841 aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg tagattttgg taccgaagaa
      901 gaagaaaaga ttgcgactgt tttgttcggt attgcaggag tcggagatga gcatttgcaa
      961 cttgtacagc aaatcgcact ttattgcagt gatatggaca atgttgtcca gctagcagac
     1021 gcgctcagca aagaagaagt aacggaaaat ctagcaattg cttaaggaga gagaaaaatg
     1081 agagcagtac attttggtgc aggaaatatc ggtcgaggat ttattggaga ggttttggca
     1141 gctaatggat ttgagattac tttcgtagat gtgaatcaca cgatcattga tgctttaaat
     1201 gaaagaaaag aatacgaaat cgaattagca gatgaatcaa aagaaaaaat cactgtaaaa
     1261 aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga aatcgctgaa
     1321 gcagatttag ttactaccgc cattggcccg aatatcttac cttttattgc tgagttgatt
     1381 gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa catctttaga tatcattgcc
     1441 tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag aagtgtataa atatcttccg
     1501 gaaacatcat ttactgacaa atatatcggc tttccaaatg cagcagtcga tcgaatcgtc
     1561 ccattacaac accatgaaga tcctttattc gtccaagtgg aacctttcaa agaatgggtc
     1621 attgacgata gccaaagaaa aaataaaaaa atccaattaa aaggggtttt atatgtagat
     1681 gatctagaac catatatcga acgcaaacta tttagtgtca atactggtca tgcgactgtc
     1741 gcttatacag gtgccttgtt aggttaccag accattgatg aagcaatgca agatgcgtta
     1801 gttgtggcac aattaaaatc agttttgcag gaaacaggaa gtttgctgat tgccaaatgg
     1861 gggtttgatg cagagcagca tcatgcgtat attgaaaaaa tcattcatcg cttccaaaac
     1921 aaaaatatct ctgatgcgat cacacgtgtc gctcggacac cattacgtaa gttaggttat
     1981 caagaaagat ttacacgtcc agtgagagaa ctacaagaac ataacttgac ttgcccacat
     2041 ttgacggcaa caatggggat tatttttaat tactatgatc ctgaagatga acaaagccgt
     2101 caattgcatg aaatgaagat ccatgaaaac ttggaacaat tgattcaaga agtcacagga
     2161 atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta tgccaagcaa
     2221 gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa a
//
LOCUS       DASJON010000277         2259 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.284, whole genome shotgun sequence.
ACCESSION   DASJON010000277 DASJON010000000
VERSION     DASJON010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.284"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(148..2145)
                     /locus_tag="U3989_002794"
     CDS             complement(148..2145)
                     /locus_tag="U3989_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN1716203.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          769 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaa
//
LOCUS       DASJON010000278         2247 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.285, whole genome shotgun sequence.
ACCESSION   DASJON010000278 DASJON010000000
VERSION     DASJON010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.285"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..545
                     /locus_tag="U3989_002795"
     CDS             <1..545
                     /locus_tag="U3989_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEN1716204.1"
                     /translation="IIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVL
                     GGSDASVPSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPI
                     MAPLADLVGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQ
                     GFLFVLGTIFIMIAIAIGLQ"
     gene            702..1886
                     /gene="iadA"
                     /locus_tag="U3989_002796"
     CDS             702..1886
                     /gene="iadA"
                     /locus_tag="U3989_002796"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN1716205.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            1997..2200
                     /locus_tag="U3989_002797"
     CDS             1997..2200
                     /locus_tag="U3989_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1716206.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT          741 a          380 c          534 g          592 t
ORIGIN      
        1 gaatcattgc tgttcttttc aaattagatg gaatgaaagt caatgacatc gcttcttctt
       61 tccaatctgg tgcagctgat ttagctggta cagcaatcgt agtaggtatg gccaaaggga
      121 ttcttcttgt cttgggagga tctgatgcat ctgttccttc agctttgaat acgattttgc
      181 atgggatcgg gacggcgttg accggtgttc cagctgtcat tggtgcttgg gcgatgtaca
      241 tcttccaaag tttgttcaac ttggttgtta catcaaattc aggacaagca gctttgacta
      301 tgccaatcat ggctccactg gcagatttag taggtgtatc cagacaagta gcagttcttg
      361 cttatcagct tggttcaggt tttatggacg cttttacacc cgtatcagcc agcttgattg
      421 gtgtgctagg tgttgcacgg atcgaatggg caaaatgggc aagattccaa atcaagatgc
      481 aaggattcct gtttgtttta ggaactattt tcatcatgat tgccattgca ataggtctgc
      541 agtagtactg gagacctgta aataaaaaag taatccaaac agtagataag gactccaaga
      601 accgtcattt tacggtaaaa tcaaaggagt aaagatccta tcgtttggat tattttttaa
      661 gatcctatca tcagttatac ttaaagaaag aggaaaaatg tatgaaaatc attcgacaaa
      721 ttgaagtgtt tgcaccagat tacttaggaa aaatggatgt actgattgcc ggtgggaaga
      781 ttttggctgt agaagaacag ctagaaggcg gctacgaagg agtggaagta gaagaacttt
      841 caggtgaggg aaaagtacta accccgggtt tcattgactg tcatttccat attttaggtg
      901 gaggaggaga aggcggttat caaaatcgaa cgccagaagt cacattgagc caattgacta
      961 cagctggtgt aacgacagtt gtcggttgtt tgggaacaga cggagaaggt agagatatga
     1021 ctgcgttgat cagcaaagcg aaaggtctgg aagcagaagg aatctccact tatgtatacg
     1081 aaggttcgta ccgattgccg gtaaaaacag taacagattc tatcatcaaa gactttttga
     1141 caattgataa aattatcgga atcggagaaa tcgcagtatc cgatcaccgt tcttctcagc
     1201 caagttttga agagttcgct cgtgcagtag cagatgctcg tgtaggaggc atgttatccg
     1261 gaaaagctgg gatcatcaat gtccacttag gcggaggaaa aagaaagcta gagctattga
     1321 cacgaatcgt agaagaaaca gaaattccga tcacacaatt tttgcctact catgcaaatc
     1381 gcacaccaga attatttgaa gcttgcgtgg catttgccaa acgtggtggc acgattgact
     1441 tcactgctag cgaagatcct gatttttggg agaagacaga tggtgaagtt cgcttcagca
     1501 aagcactgaa acgattgatc gaggaagatg tttctttaga taatttcacg atgacctcag
     1561 atggacaagg aagcttgcct tattttgatg aaaacaatca tttcctaggg ttaggagtag
     1621 gaagtgccaa ggcattgcta gtagggatca aagaagctgt gcaaaaagaa agtatccctt
     1681 tggaaatcgc cttacgagct ataaccagta atccagcacg tatattgaaa ttggataaaa
     1741 aaggaaaaat cgaaattggt gctgatgcag atctgtgtat cttggataaa gaaacgcttg
     1801 atattgatac agttatcgct aaaggagaaa taatggtcca agaaaaagaa gtcaaagtat
     1861 gggggacttt cgaaaaaagt ttttaatcaa cagctctaac tatctttttt agaaaacgag
     1921 cgttctgttt gtctgtatct gttgttgatg ttaccctgaa ggtagatcaa taaacaaact
     1981 aagaggagga agcacaatgg cagatttaaa aggacgttct aaagatatga aagataaagt
     2041 agtcggtgaa ggaaaagaag catacggcaa agcaacagac gacaagagca aagaaacaga
     2101 agggaaagca caatcactag gtgctgacat caaagaaaaa gctcgaaatt taggcgacga
     2161 agtcaaagac aagttttctc gaaaagacga aaaaaaataa aaaccactta ttactaattg
     2221 aatcaagcaa ataaaaaatc cgaacca
//
LOCUS       DASJON010000279         2230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.286, whole genome shotgun sequence.
ACCESSION   DASJON010000279 DASJON010000000
VERSION     DASJON010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.286"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="U3989_002798"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="U3989_002798"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN1716207.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..1406
                     /locus_tag="U3989_002799"
     CDS             1131..1406
                     /locus_tag="U3989_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716208.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            1833..>2230
                     /locus_tag="U3989_002800"
     CDS             1833..>2230
                     /locus_tag="U3989_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1716209.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWV"
BASE COUNT          719 a          433 c          336 g          742 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtcata
     1381 aactctatgt gaatcagcaa ttttaacttc taactgttct ttattaatta aaaatgttct
     1441 gtaataaaat aaaactttat aacaactata acaaaaccga atgcaaaaca gaagttctat
     1501 aagttataat catagttgtt aggtgattca tctagtaaaa acaccagatt tcaaatttca
     1561 cacaatttaa actagatcaa tcatcaacct tcttcaaatt gttagaaccc tttcccgagg
     1621 agctgcagca taagcaaaaa aacaaacagg aagccaaatg gctaagacat tttttctatt
     1681 agtctagttg cttctcgtcg caagtttaag actttagaaa gttatttcta atgatgtttg
     1741 ggttgttttg atcaatgctt atgcacagct ccttacctat tatttatctc aacagatcta
     1801 acaaattaat ggactattta aggagttttt ttatgcttac gatctattct tctcgcacac
     1861 atagttacca tctgatggca aactggctga aagaccatca aatgccttac agagaacgaa
     1921 ttttcacacc taaacagcct ttaacaagga aagagatatt ttatttattg tcattaacag
     1981 aaaacggatt tgacgatcta ttagctaaac gctcgattga ttataaaaat ttgaaagacc
     2041 aaatcgacca atgtacgacc tcagagcttg tcgatctgat cgtctcgcat tttactttgt
     2101 tacaacgtcc catcattact gacggaaaga aattattagt cggattcaat gaagatagca
     2161 tgcgtctttt tatccctgta caatggcgca gaagtcaact gaaatctgtc tataacgatt
     2221 ttacatgggt
//
LOCUS       DASJON010000280         2225 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.287, whole genome shotgun sequence.
ACCESSION   DASJON010000280 DASJON010000000
VERSION     DASJON010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.287"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1507)
                     /locus_tag="U3989_002801"
     CDS             complement(<1..1507)
                     /locus_tag="U3989_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1716210.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTE"
     gene            complement(1622..2170)
                     /locus_tag="U3989_002802"
     CDS             complement(1622..2170)
                     /locus_tag="U3989_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN1716211.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
BASE COUNT          648 a          507 c          390 g          680 t
ORIGIN      
        1 cttctgtatc aagacctaat cggctagaga tttcaaacgc attgcttcgt ccaggaacgc
       61 caatcagcaa acgatacgtt ggacttaatg tatccacatc aaactccatg ctggcattaa
      121 tcgtatttgc ccgattatat ccataaactt tcaattcagg ataatgcgtt gttgccatca
      181 cataagcaga ctttttacca agatcatcta agattgcgat tgctagagca gcaccttctt
      241 gcggatctgt tcctgcaccg agctcgtcaa ataataccag acttttttca ttgacttttg
      301 ataaaatatc tacagtgttc gtcatatgag aagaaaaggt cgatagactt tgctcaatcg
      361 actgttcgtc tcctatatcc gcaaatactt cctcaaaaat accgatttga ctttcttcac
      421 ctgcaggaat cggtaatcct gcttgcccca tcagttgtaa cagacctaaa gtcttcaacg
      481 tgatcgtttt cccacctgtg ttaggtcctg taatgacgat ggcttgatag tcttttccga
      541 ttgtgatgtc attagaaact actttttcct gatcgatcag tggatgtctt gcttgtttca
      601 agacgacatg attgtcttca ctgattcccg gcacgatggc ttttacttct ttaccaaatc
      661 gtgcttttgc attcatcaga tccattttac caatcacata agtattgtgg atgatttctt
      721 gacgatgggg aacaagttca gcagaaagtt cacttaaaat acgttgaatc tcatttcgtt
      781 cagcgatttg gtattgacgc aaacgattat tcaaatccac cacttgtttt ggttcaataa
      841 ataaagtctg acctgaagca ctttgatcgt ggactacacc gccaaataca ccgcggtatt
      901 cttgtttgac tgggataacg tagcggtcat ttcgcatggt gatgatcgca tcacttaaat
      961 actttgcatt tttcccacgg acgatcccat ctaactgttc acgaacagct tgttcacttc
     1021 gccgaatgtt ctgtcttatg ctctttaact caggagaagc gtcatcagtc acgcgtccgt
     1081 cttcatcgat tgcttccttt aaacggcggc tcaaaacagg gatcgtgact aactgatctg
     1141 cccacatata caaacgtgca aattcgattt cactgtcaga aagatcatca atgaaacgat
     1201 tcaattcgct tgtcgtagac aatactctgg aaacttgtgc aagctccact ccattcaagt
     1261 ctgctccgat ctcaatccgt ttcatatggg gacggatatt ttcaatttta ggtataggga
     1321 tcccgcctcg cagacgttgc actttcaatc catcctcagt ttcttgaagc cagtttgtga
     1381 tcgtttgttt ctcactcact ggtaccaatt ctgccagttc ttcttttcct tgtgcagtca
     1441 ctacaaattg tctgaccatc tgtttgactt tttcaaattc caatgtttca aggattcggt
     1501 tattcatttt atcaccttat ctttcactat gaaaaaagag ctgtgccgga catacgctac
     1561 gcagccttct taaataaact actctgtaca aaaaacgaaa tattttccag cactattttc
     1621 cttaattcaa aatattcgtt acccaaagct catacatctt gttggtaaga aatggcgttc
     1681 tctcaacgat aaaacgagcg aaactatgct cgccgaattg attttggacc acatctaatg
     1741 gaacaaaggc aagtaactgc aaaatcaaaa atatagtaac gtaagccatg attagtgaca
     1801 ggatgcctcc tgcaatccag tctacctgtc tcaagatcgg cacatacgtc aatccatgga
     1861 caaaaacccc cacaaatcgg gtaagcagcc agccaattag caaaataatc agaaaagcag
     1921 ttcctgcata aaaagcttca tctaggtgaa aggcaaatac ctgatcaaaa aatgctaact
     1981 ttgatgttgg ggtgacagct gggtatggaa tgtataattc caaatgactt gctaatggtt
     2041 tgtaaaaatg ctgggcagca ataaatgaca atacatatcc aatcgcgaat atgatttgca
     2101 aagcaaaccc gcgacgtgct ccagaaaaaa acgcaattag caataaaaat aaaatcagca
     2161 aactcagcat gctacgttcc tttctcttct aactttttta aatttttctt attttgataa
     2221 aagga
//
LOCUS       DASJON010000281         2205 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJON010000281 DASJON010000000
VERSION     DASJON010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.288"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="U3989_002803"
     CDS             complement(<1..914)
                     /locus_tag="U3989_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN1716212.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="U3989_002804"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="U3989_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN1716213.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="U3989_002805"
     CDS             2006..2176
                     /locus_tag="U3989_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716214.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          747 a          384 c          421 g          653 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtata
//
LOCUS       DASJON010000282         2189 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.289, whole genome shotgun sequence.
ACCESSION   DASJON010000282 DASJON010000000
VERSION     DASJON010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.289"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..406)
                     /locus_tag="U3989_002806"
     CDS             complement(<1..406)
                     /locus_tag="U3989_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="similar to full-length Gnd, these proteins seems to
                     have a truncated C-terminal 6PGD domainin; in
                     Methylobacillus flagellatus this gene is essential for
                     NAD+-dependent oxidation of 6-phosphogluconate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HEN1716215.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKL"
     gene            405..1253
                     /locus_tag="U3989_002807"
     CDS             405..1253
                     /locus_tag="U3989_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1716216.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1899..2105
                     /locus_tag="U3989_002808"
     CDS             1899..2105
                     /locus_tag="U3989_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN1716217.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT          691 a          383 c          339 g          776 t
ORIGIN      
        1 aaagttttct taacatgttt tcggttatct ctccagcagg aagcattaac cagatgattt
       61 ttttctgctc aaaagtagct agaaattcag agatctcttg ataagcagga atgtcatttt
      121 ctcttgcctg actaacagct gtacttgaaa tgtcgagtcc ttgtactttg tgatggtgct
      181 cttttaagtt taaagctaaa ttcaatccca tttttcccaa accaattaca ccaatttcca
      241 tgatagcgcc tccatttctt ataccctcat tatacaaaaa aatttttttg taaatagatt
      301 ttacaaaaat attttttgtt tgattttttc ctactctcct tgtatcttta acattaatga
      361 gaacggaaaa aatgataatc ttattatgaa aatgaggaat tctcatgagc gtctccataa
      421 gactacaaat caaaacaatg tacaacgact tcagcccaaa agaacaggct atcgccgatt
      481 atattttaga aaatccaagc aaagtaagtc atagtcctat tagcaattta gcaagtgaac
      541 tgggcattgc tgactctact ttttttcaat ttacaaaaaa acttggttat aatggattta
      601 aagattttaa aatggccatg ttgatccaag aaaatgatct atctgctatt tctatccacg
      661 aaaatattca gaaaacagat actgagctca ctatggcgca aaaggtgttc gagtctagta
      721 tttcaacttt aaatgacaca aaaaagttgt tacaacaaaa ggatctaaaa aaagcggcta
      781 ctattatcaa tgaatctaaa cgtgtctatt ttttcggtgt tggcggttca gagattgtag
      841 caactgacgc ttatcataaa tttttgcgtt cccctatcgc aacttctcac agcacggatt
      901 accatattca attgatggaa gcttctttat tgacggaaaa agactgtgct gttttgattt
      961 ctcacacagg gcaatcaaaa gaaacaatcc atattgctga aaccgtaaga aaaactggag
     1021 caaaaacaat cgttattact agccaagcta attcttccct tgctaaactt ggcgatgtcg
     1081 tttttatttc tatttctgaa gaaacagaat tccgatctga agctttagct tcccgtattt
     1141 ctcaactaag cattttggac tctctatatg tgattttgat gttttacaac gaaaaagaat
     1201 cacgtcgttc tatcgctaaa gttcgacata ctatttctga aatcaaagaa tagcaccata
     1261 acagaaaaag cgaaaaatcc ataaaacaaa aaaagccact accaaatatt tggtagtggc
     1321 ttttttggtt ctgcaagggt ggtacagaac atcaatagtc ggtgggttag aacgtatgtc
     1381 atcgcatctt tggagaaggt tgtattggta ttattcgagt tacttgttag atgcgataac
     1441 aaagttagta tacagtagta aaaaatttat cttttctttt ttttattatt tttgtttctt
     1501 attttctaaa aaattaaaaa aaattttggt gaattaagta tttccttgat aacactggct
     1561 tttggcctca ttttaggtta tcatttgtaa ttttatttca cagttgatat gattgttatg
     1621 taaaataaaa aagtttagga tacatcgaat ttttggggaa attcttttgg gggtatccta
     1681 aatcttttct attatattcc catattttct attattggtc tagacgtatg atggtaaact
     1741 ttgtacctat ctgattgctg aatcattaat tgcttatcat tagccatctt tgtttcaagt
     1801 tcgctagatg atcattttat tcacatccta acaaacttgt cacatttata gattcttaat
     1861 ctatttatca tacaattata aaaaaggagg tgctgcttat gaaagctatc catatttctt
     1921 tgcttttttt cgctagtgca ttgattttat ttgctattca tctttgttcc cagccttata
     1981 tcaataacaa cggaatatta gtggaaccag cattcttttg cttaccactc ggctttttgt
     2041 cccttcttgc ctccgcaggc ttcggatttg ttgctttttt taaacgatca aaacaacaaa
     2101 tctgacttat aaaatttacg taaaagagat cgtgacaata atcgtgtgat attcccaaat
     2161 ttcgaataaa aggtgcccct tgccatctc
//
LOCUS       DASJON010000283         2178 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJON010000283 DASJON010000000
VERSION     DASJON010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.290"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..2018
                     /gene="tkt"
                     /locus_tag="U3989_002809"
     CDS             21..2018
                     /gene="tkt"
                     /locus_tag="U3989_002809"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN1716218.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
BASE COUNT          696 a          410 c          498 g          574 t
ORIGIN      
        1 aattaatagg aggggttatt ttgtttgaca aaattgacca acttggcgtt aacacaattc
       61 gtacattaag tattgaagca gtccaaaaag caaactcagg acatcctgga ttgccgatgg
      121 gcgcagcacc tatggcatat gcgttatgga caaaacacct gaaagtaaat ccaacgacat
      181 caaaaaactg ggctgaccgc gaccgtttcg ttctttcggc aggacatggg tcagcaatgt
      241 tgtatagctt gcttcattta gctggctatc aagtgacaat cgatgatttg aaacaattcc
      301 gtcaatggga ttctaaaaca ccaggacatc ctgaagtaca tcatacagat ggtgtagaag
      361 ccactacagg gccattagga caagggattg caatggctgt cggtatggca atggcagaag
      421 cgcaccttgc agcaacgtat aacaaagaaa actttaatgt gatggatcac tatacgtatg
      481 caatatgcgg tgacggagac ttgatggaag gggtatctca agaagcaagt tcgatggcag
      541 gtcatatgaa actagggaaa ttgatcgttt tatacgattc aaacgatatt tcactagacg
      601 gtccaacttc aaaagctttc acagaaaacg ttggtgctcg ctatgaagca tatggctggc
      661 aacatatctt agtgaaagac ggaaatgacc tagaagcaat ctctaaagcg attgaagaag
      721 caaaagctga aacagacaaa ccaacattga tcgaagtaaa aacagtcatc ggctatggtg
      781 ctcctaaaga aggaacatct gctgttcatg gtgctcctct tggtgaagat ggcatcaaga
      841 tggctaaaga agtttatggt tgggagtatc cagatttcac tgttccagaa gaagtagctg
      901 cccgcttcca tcaaacgatg atagaagaag gacaaaaagc tgaagatgca tggaatgaaa
      961 tgtttgccaa ttataaaaaa gcttatcctg aattagcaca acaatttgaa gatgcttttg
     1021 atggcaaact accagaaaac tgggatgcag aattgccaac ttatgaagtt ggaagcagtc
     1081 aagcaagccg tgtttcaagt aaagaagtca tccaagaatt atcgaaagct ataccaagct
     1141 tctggggtgg ttcagcggac ttgtcaggtt ccaataatac aatggtggct gcagataaag
     1201 atttcactcc tgaacattat gaaggacgca acatctggtt cggtgtccgc gaatttgcaa
     1261 tggcttcggc tatgaatggt atccaacttc atggcggaac tcgtatctac ggtggaacat
     1321 tctttgtctt tgtcgattat ctaagaccag cagttcgttt agcagcaatc caacatactc
     1381 ctgttgttta cgtcttaaca catgactcgg ttgctgtcgg agaagacgga ccaacgcacg
     1441 aaccgattga acaactagca tctgttcgct gcatgcctgg tgttcaagtg attcgtccgg
     1501 cagacggaaa tgaaacacgt gctgcttgga aagtagcgat ggagacaaca gatgcgccta
     1561 ctatccttgt attgagtcgt caaaaccttc cagtattgcc ttctacaaaa gaagtagcag
     1621 atgaaatggt gaaaaaaggc gcgtatgttc tttctccatc acaaggagaa acaccagaag
     1681 gtattttgat tgcaacaggt tctgaagttg atttggctgt gaaagctcaa aaagaattgg
     1741 ctgaaaaagg caaagatgtt tctgttgttt ctatgccaag ctttgacctc tttgaaaaac
     1801 aatcttctga atataaagaa agtgtacttc ctaaatcagt gaagaaacgt gtggctatcg
     1861 aagctgctgc atcatttgga tgggaacgct acgtaggaac agagggcgca acgattacaa
     1921 tcgatcattt cggtgcatct gcccctggca ctaaaatttt agaagaattt ggatttacag
     1981 tagaaaatgt cgtgaatacg tacaatcaac tatcatagat ccaagcaatt gtttgactga
     2041 taagaacagc tgtctgcaag ttggagacag ctgttcttta tgtgttagaa gaataaatca
     2101 atattacttt tcgttttcgg ctatgttaca tttaaatgac aaaacaaaga attcttcgga
     2161 ttagtttgtg tttcttga
//
LOCUS       DASJON010000284         2105 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJON010000284 DASJON010000000
VERSION     DASJON010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.291"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            153..1313
                     /locus_tag="U3989_002810"
     CDS             153..1313
                     /locus_tag="U3989_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1716219.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1329..1772
                     /locus_tag="U3989_002811"
     CDS             1329..1772
                     /locus_tag="U3989_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1716220.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          725 a          349 c          412 g          619 t
ORIGIN      
        1 aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg caggacagct
       61 ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
      121 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
      181 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
      241 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
      301 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaacc
      361 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
      421 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
      481 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
      541 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
      601 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
      661 gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt aaaaatatac
      721 aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa gacttagtta
      781 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
      841 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
      901 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
      961 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
     1021 ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa cataagaaaa
     1081 aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat gggaaagtca
     1141 agctggatca attgcctggt ggaacttatc gaatcgtttc agctgatgat cctaaagttg
     1201 ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt gaggggcgaa
     1261 aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa taagtaagga
     1321 gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa tctgttctat
     1381 cgatttggct gcatgctaac ttggattctc atgaattcat tccatcaact tattgggaga
     1441 atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta gcagaaatag
     1501 atcaagaaat tattggtttt gctggggtga ttgatcagtt tatcgcagga atctttgtga
     1561 aaagggaata ccggcaacaa agaatcggta cgcaactaat gaaaaaaata aaagaaacca
     1621 ataaaacact gactttgaca gtctatcaaa aaaatcaatc agctattcat ttctatcttt
     1681 cacaaggttt tacaattcaa acacagcaaa aagatgaagc agtaaatgag gaggaacttg
     1741 tgatgaaatg gaatcaaaac gctgattttt gatgaacaga ataacgaaat aatgctccca
     1801 gcctaatttt ttgctgggag cattatttca ttttgaagtc actaatttaa ctgtgtgaat
     1861 atgcagttgt tacaatcttt gataaacggt gttcaatcag ctgaccctcg gtattagctc
     1921 aaaaaacggc aaaatatgag gaactatttt tccatttgtt tctctaatac tcaggtcaaa
     1981 acgctgattt cacaacctct tgataaacgg tgttcaaacg gctgcttctc ggtaataagc
     2041 ccactagcct agaaaatatg aaaagctatt ttctaactag cgttcttatt actcaaagca
     2101 taacg
//
LOCUS       DASJON010000285         2022 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJON010000285 DASJON010000000
VERSION     DASJON010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.292"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(544..909)
                     /locus_tag="U3989_002812"
     CDS             complement(544..909)
                     /locus_tag="U3989_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716221.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(985..1230)
                     /locus_tag="U3989_002813"
     CDS             complement(985..1230)
                     /locus_tag="U3989_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1716222.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1300..1554)
                     /locus_tag="U3989_002814"
     CDS             complement(1300..1554)
                     /locus_tag="U3989_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716223.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1718..1930)
                     /locus_tag="U3989_002815"
     CDS             complement(1718..1930)
                     /locus_tag="U3989_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716224.1"
                     /translation="MIFQGVITLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIIL
                     SFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          744 a          350 c          364 g          564 t
ORIGIN      
        1 aaaaaagaaa gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa
       61 ctatttttac tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca
      121 actgaactga tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat
      181 cacagtgact acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt
      241 aagcatcgat gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag
      301 atcaaaagaa gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta
      361 aaccacgtca ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag
      421 tacgctttta gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct
      481 tgatctaaga tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt
      541 atattaggta agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac
      601 ttcatcgtgt tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc
      661 ttcgcatgat ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg
      721 tttttccatt tcatccatga caagctgatg atattggaat aatctataag gagaatagtg
      781 aaaaacataa tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca
      841 ttcccgatgt tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca
      901 taatcgcata gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa
      961 aaccagcacc aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat
     1021 taggtaatca acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg
     1081 aatttcttct ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga
     1141 aacagctact tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt
     1201 atccagtaaa atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt
     1261 ataatgacta tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg
     1321 tatcaaataa agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa
     1381 aacactatcg ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg
     1441 aagagtcaaa atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact
     1501 tgcgaccagt gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg
     1561 gactcctcaa cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa
     1621 tgctaggaaa ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg
     1681 ctgataagca ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat
     1741 tgatatctaa gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa
     1801 gaatgatgat cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg
     1861 catatctttt tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt
     1921 gaaaaatcat tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag
     1981 ctgctgccgc aactaaatcg aataaaatcg caataaatgg ca
//
LOCUS       DASJON010000286         2020 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJON010000286 DASJON010000000
VERSION     DASJON010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.293"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U3989_002816"
     CDS             200..742
                     /locus_tag="U3989_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716225.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="U3989_002817"
     CDS             898..1347
                     /locus_tag="U3989_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN1716226.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2020)
                     /locus_tag="U3989_002818"
     CDS             complement(1377..>2020)
                     /locus_tag="U3989_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEN1716227.1"
                     /translation="KRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          730 a          316 c          385 g          589 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca
//
LOCUS       DASJON010000287         2019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.294, whole genome shotgun sequence.
ACCESSION   DASJON010000287 DASJON010000000
VERSION     DASJON010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.294"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..570
                     /locus_tag="U3989_002819"
     CDS             100..570
                     /locus_tag="U3989_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN1716228.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="U3989_002820"
     CDS             792..1895
                     /locus_tag="U3989_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1716229.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DASJON010000288         2018 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJON010000288 DASJON010000000
VERSION     DASJON010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.295"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..344
                     /locus_tag="U3989_002821"
     CDS             <1..344
                     /locus_tag="U3989_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1716230.1"
                     /translation="LEIPAGKIDPGEGQNPEMTAARELEEETGYRAKSLSHLTSMYLS
                     PGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQQAMKDQLICDAKTIYA
                     IQYWELLTKGK"
     gene            357..641
                     /locus_tag="U3989_002822"
     CDS             357..641
                     /locus_tag="U3989_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716231.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            660..1352
                     /locus_tag="U3989_002823"
     CDS             660..1352
                     /locus_tag="U3989_002823"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN1716232.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            1364..1909
                     /locus_tag="U3989_002824"
     CDS             1364..1909
                     /locus_tag="U3989_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1716233.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
BASE COUNT          706 a          354 c          446 g          512 t
ORIGIN      
        1 ttttggagat tccagctgga aaaatcgatc caggagaagg acaaaatccc gaaatgacag
       61 cagctagaga attagaagaa gaaactggct atcgtgcgaa gtcgctgagc catcttactt
      121 cgatgtatct ttcaccaggt tttgcaaatg aagtccttca tatttatcat gcacaagggg
      181 tagagaaggt cgagaatcct ttagcacaag atgaagacga agtactggag ttgtatcatc
      241 taactcttga agaggcgcaa caagcgatga aagatcagtt gatttgtgat gcaaagacga
      301 tttatgcgat tcaatactgg gaactattga ccaaaggaaa gtagagggat cggcttatgg
      361 ccaaaggacc gcttattacg cgaagcgaac ttcgcaagcg acaacaagca caagcaagcg
      421 agtcattaaa aaaacagcga aaagcagaaa cagcttatca gcaagaagaa aaaaagattg
      481 ccagctttta tcgtaaagaa agcaaaaaga ataaaccaat cacgaaaaca aggatcagcg
      541 aacgggaaaa gacgaccaaa tggaactctt ttttgatgaa atctcttatt attgttatcc
      601 taatgttatg tgtggttttt ttagcaatcg catttatata gaaagaagga attaaaaaga
      661 tgaaaatcgg tattatcggc gcaatggaag aagaagtaaa aattttgaga gagaacctaa
      721 gcgaaccatt atcatgggaa cgcgcaggcg ctttatttat ttcaggttca ttaggtaacc
      781 acgaagtgat cgtcgtacgt tccggtatag gaaaagtctt agcatctatt accacaagtc
      841 tgttgatcca acaatacggc gtgaacatgg tgatcaatac aggttcagct ggcggaatcg
      901 gtgaaggctt acaagtaggt gacatcgtta tatcagacaa agtagcttat tttgatgcag
      961 atgcaacagg atttggctac aacccaggac aattaccagg aatgcctcta tattatgaag
     1021 caagcacata tttgcgcagt gaaatggtta aagcagcaaa agcaacgaat ttgaacgcaa
     1081 aagaaggatt gatcgttaca ggtgatacat ttgtcgactc gccagataaa atcaaagaaa
     1141 tcttaacgaa tttccctgaa gcattagctt gcgaaatgga aggagccgct gttggacaaa
     1201 cagctcgtca gttcaatatc ccatttttga tcgtacgtgc aatgagcgac acagcagatc
     1261 actcggcgac acaaagcttt gacgaattta tcgaagacgc aggcaaacga tcagcagaaa
     1321 tggtgatcga atttgtcaaa catctcgtat aaggaggact aagatgaacg cattgatctc
     1381 gattgactac acatatgatt ttgttgcaga ggatggaaag ctgactactg ggacagcagg
     1441 acaagtaatt gaaaaaaagc tgactgctca tacaaagaag tttattgatc aaaaggattt
     1501 tgtcgttttt gccattgatg cgcatgatcc aaaagattcc tttcatccgg agaaccgctt
     1561 atttcctccg cataatgtga taggaacggg tggacgggaa ttgtacggct ctttaaaaag
     1621 tttataccaa gactatgagc aagaggaaaa tgtttattgg atagacaaac ggcattattc
     1681 cgcttttagt ggaacagatc tagacatccg tttgcgagaa cgccagatca cagatattta
     1741 tttgaccggc gtatgtacag atatctgtgt gttgcataca gcagtcgatg cgtataatct
     1801 aggatacaaa ctccatattt tcaaagattc cgtggcaagt ttcgatcccg ttggacatga
     1861 atgggcattg agacattttg agtccacact aggcgcagaa attttataaa aaaacaaagt
     1921 gatttgataa aataaaaagc acaaaataat aaaagaagag cataaaacaa acgactttgt
     1981 tttatgctct tttttatacg ttattttaaa aaaactta
//
LOCUS       DASJON010000289         2003 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJON010000289 DASJON010000000
VERSION     DASJON010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.296"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U3989_002825"
     CDS             <1..378
                     /locus_tag="U3989_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1716234.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U3989_002826"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U3989_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1716235.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U3989_002827"
     CDS             1859..>2003
                     /locus_tag="U3989_002827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716236.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASJON010000290         1996 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.297, whole genome shotgun sequence.
ACCESSION   DASJON010000290 DASJON010000000
VERSION     DASJON010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.297"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..1365)
                     /gene="glnA"
                     /locus_tag="U3989_002828"
     CDS             complement(25..1365)
                     /gene="glnA"
                     /locus_tag="U3989_002828"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN1716237.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1393..1785)
                     /locus_tag="U3989_002829"
     CDS             complement(1393..1785)
                     /locus_tag="U3989_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1716238.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          567 a          442 c          336 g          651 t
ORIGIN      
        1 cctctaattc acggtattcc tgttttaata aagttctaga tattgttctc tttcccactc
       61 ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa aatttgagaa
      121 gatatgttgt cctaatgcgt caatcattac attatcctta cgcaattctt tgatagcatt
      181 atggatagtt gaaggtaagt cctggatatg tgcagcttct ctttcttcct catccattac
      241 ataaatgtta cggtcaacag ctgctggcgg tgtgatctga ttacggatac cgtctagtcc
      301 tgcttgcaac aatactgcca ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa
      361 ttccaaacgt gtggataaac cgcgagattc tggtacacgt actaatggcg aacgattacg
      421 gccactccat gcgacataaa caggcgcttc ataacctgga acgagacgtt tataagaatt
      481 gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa
      541 atgataagct gtttcactta gttccaattc accgtcttta tcatagaaaa catttccttc
      601 atcattgaac aaagacatat tgcaatgcat accagaacca ttgatcccaa ataacggttt
      661 cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa
      721 ggtttggata ttatcacaag catctaccac atctgcatat ttaaagtcaa tttcatgctg
      781 tcctggcgct acttcatgat gtgaagcttc cacttcaaag cctagacttt ctaattccaa
      841 cacgatgtca cggcggcaat tttcacctaa atctgttggt gcaaaatcaa aataaccacc
      901 tttatcattc aaggtcgttg tgatcccacc gtcttcgtct aatttaaata agaagaattc
      961 tggttcaggt cctaaattga aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg
     1021 tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca tccggattat agatatcaca
     1081 aattaaacga gcaacttttc catgagcact ttcccacggg aagatcatcc aagtggagag
     1141 atctggatac aaatacatat cgctttcttc aattcgaaca aatccttcaa tcgaagaacc
     1201 gtcaaacatc attttatttt ctaatacttt gtcgatctgg ctcactggaa cttctacatt
     1261 tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc
     1321 aatgattcgt ttgatttctt cacctgttat ctgtttcttt accatggtat ccctcttcct
     1381 tttttaattt attcagtcaa agaattgtct ttgcaatcct tgcgactgat aatgacctgt
     1441 tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc
     1501 tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg
     1561 tttgatccca gccatattca atccctctga aagatagtct ttaatctcaa gcaacatatc
     1621 aatatcattc agcgaataca tacgtcggtt tccttcgctt cgttccggat gaactaaatc
     1681 ttgttcttca taatagcgaa tttgccgagc agacaaatca gtcagcttca taactgtacc
     1741 aattggaaat acggacatag atcggcgcaa ttccttttct cccatcagac gtcccctttc
     1801 gttttataag ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg catatgttag
     1861 gttttctaac atgagatctc tattttttat aaaataacaa aaaaataaga acacaaaaaa
     1921 ggtatctagt tgattttcgt accagatacc ttttattttt tattatactg tactatcctt
     1981 tattagtgtt tttatt
//
LOCUS       DASJON010000291         1992 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.298, whole genome shotgun sequence.
ACCESSION   DASJON010000291 DASJON010000000
VERSION     DASJON010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.298"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1240)
                     /locus_tag="U3989_002830"
     CDS             complement(<1..1240)
                     /locus_tag="U3989_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEN1716239.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDSEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENF"
     gene            1688..1861
                     /locus_tag="U3989_002831"
     CDS             1688..1861
                     /locus_tag="U3989_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1716240.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
BASE COUNT          542 a          421 c          354 g          675 t
ORIGIN      
        1 ggaaattttc tggatttttc tcaatgatct cgcgaacgaa tttaccaagt tcggtcatat
       61 cttctgcttt aacggagcct ggtttttcaa attgcaaagt atattttttc caatctggca
      121 taataagcgg tcttggatcg attccaccat tggtgatagg attttttgcc attctttggt
      181 caccagtagg cgcgatttct ctgatttctt ctaaaatacg tccttgtgca tcaaataatt
      241 tttctggttc ataggatttc agccaacgga gtaaagcatc tgcatgatcc atatgttctt
      301 gatctacagg aatcggtact tggtgtgctc ggaaggttcc ttcaattggt tccccgtccc
      361 attcttttgg accggtccat ccttttggtg tgcgtacgat caatactggc cattttggca
      421 tcacgacatc tgttgctgtc ttcaggcgag cttctttttg gatggattta attttctcga
      481 ttgcttgatc cattttttca gccataacag gatttaattt ttctgaatca tttccttcaa
      541 taaagaaagg ttcccagcca agtccgttaa agtaatttgc taattctcca tctgatttac
      601 gtgccaagat cgttgggttg gctatttttg caccatttaa atgaaggatc ggtaaaacag
      661 ctccatctat cactggattg ataaagacat ttgagaacca gcttccagct aaaggaccgg
      721 tttctgcttc accatcacca atcacgacag cagagattac ttcaggattg tctaatacag
      781 ctccgactgc atgtgaaaga gagtatccaa gctctccgcc ttcatggata gaacctggtg
      841 tttctggtgc tgcatgagaa gcaatgcctc caggaaatga aaatcgtttg aataattttt
      901 gcattcctgt ctcatcttcc gttacttctg gatagatctc tgtgtaactt ccatctaaat
      961 aagcattcga taccatgact tgtccgccgt gtcctggccc ttcaatataa aacatgttca
     1021 aatcatattt attgatgact cggttcaaat ggacataaat aaaattttgg ccaggaatcg
     1081 ttccccaatg accgattgga tgttttttga catcttcggg tttcagtgta cgtctcagta
     1141 aggggttatc tttaagataa agttgcccaa cagaaagata attagcagca cgccaccaag
     1201 ccgtcatttt atcaaagtat tctttactag aataatccat acataacact ccttttcatt
     1261 attactctta ttctagcgca gatactccca attgaaacct aataagcact tctgttcatt
     1321 tgtttggatt ttatatctag ccaaatgaac aggtataaaa aaccacgaaa tttttccttt
     1381 cgctgacgtt ttttacattg atccaaaaaa cttgtcgtta tcacaatggc tgttccgcag
     1441 ttcatttcgc tcctcatcat gcataatttg ctgaatgacg ctgggccagt taacacattg
     1501 ctggaaaact ggggctggat cgagcaattc ctttcctgat tgatccgttt tatgtaaaag
     1561 tctctgttat ctttgtcaat atgtgaattg gtatcccagc tactatgcta atttctacag
     1621 ggattatcca aaatttggat cgtgaccaaa tcgaagcggc gtaatcggaa atatcaataa
     1681 cttcaacgtg atttttcttt taactgtcaa cggaccgaca aactcaaact tttatggtgc
     1741 aggttccact gatcttctag ttacttggct atacctgact gtcactaaaa tagattacaa
     1801 tctagcttcc gttatcggta tcttgatctt tatctatatg caaaaattta tatactacta
     1861 gaaaaaagaa agtcgaattt tttacttttc tttttctctt ttttggttag ctgacacgcc
     1921 gcaagactaa agtcatggaa ctcttaggta atacgtctta atgaaaacaa aaaaatcccg
     1981 caacccctga tt
//
LOCUS       DASJON010000292         1979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.299, whole genome shotgun sequence.
ACCESSION   DASJON010000292 DASJON010000000
VERSION     DASJON010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.299"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="U3989_002832"
     CDS             complement(325..528)
                     /locus_tag="U3989_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716241.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="U3989_002833"
     CDS             complement(728..1744)
                     /locus_tag="U3989_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1716242.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DASJON010000293         1948 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.300, whole genome shotgun sequence.
ACCESSION   DASJON010000293 DASJON010000000
VERSION     DASJON010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.300"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            180..482
                     /locus_tag="U3989_002834"
     CDS             180..482
                     /locus_tag="U3989_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716243.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(998..1071)
                     /locus_tag="U3989_002835"
     tRNA            complement(998..1071)
                     /locus_tag="U3989_002835"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1035..1037),aa:Ile,seq:cat)
     gene            complement(1626..1940)
                     /locus_tag="U3989_002836"
     CDS             complement(1626..1940)
                     /locus_tag="U3989_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716244.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          708 a          290 c          301 g          649 t
ORIGIN      
        1 tttataaggg tttgagcatt ttttattttc gcagattttc gttaaatgaa tgaaaatggc
       61 attcaaaata gcattcaaaa agaacacttt ttaatatata tgttccatcc atattatcaa
      121 tgatattcct actttacaaa aagaacatta gttcgtatac tctttttgag gtgaacatta
      181 tgttgatgga aggaaaaaca caattatggt ttaaatttga tccttcgaat agatttgtca
      241 aagattttta taaggtgtgg gattcagaag ttttcttttt agcaatcgaa gatagcttat
      301 taatcaatct ctattactct aataagaact actttaaaat tccggcaaca aaaaccagaa
      361 tgaagaaaga tgtgtatttt ttgtttgata tcgtgactga tgtgccagac gcacggagcg
      421 atcatcggcg ttatgactat ttgaagtata ctttcgttga tccagaaagg tataaagatt
      481 aaagtaggct acctaaaaaa ggtagcccgg aacggatttt atcaccatac ttataaaagg
      541 gatatttttt aagttagtat taagattgtg taatatgatg atatctatat tttatagtat
      601 cagtgctata aaatcaaaaa taagtcacta attaactacc actccaatta taagtctttt
      661 tcccattatt ttttacagtt atatggtatg ctttttaatg gcttcaaata taaaagagtt
      721 taaagcgtaa cacacttatg gggaagtggt ttttggggaa cgctttaaac tcttctttat
      781 tattatctca caatataacc caaatgtctt tccatttaaa aatcaaagta aaacttttca
      841 aatatacaga agtataacta tgtgaaacat cctttcatta atccataaaa ggatacataa
      901 aaaagccact catttgagtg gcaatgaaga aaagctttag cttgtataat actcttcaaa
      961 aaattctaac acagaacgat tcaattggct acattaatgt accctgtagg actcgaacct
     1021 acgaccggac ggttatgagc cgtctgctct gaccaactga gctaagggta gtggttgttg
     1081 ccacataaag ccataaacaa tcaaccagta gaatgcgtgg caacaaacct gttatcgcat
     1141 atcttggagt gtgactattt atgagtgata gtgaagatat gcgtctccat atatgtatgg
     1201 ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca
     1261 aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat
     1321 tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa
     1381 gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac
     1441 aattatggtt taagcatgtt ccttcaaata agttttttaa ttataaactc tagagaaatt
     1501 aattcatcct aacataaaat aattgttcac tcaaaagttt ttctagaaca attaaaacag
     1561 caggcttcac attcgctata catttgtaag aagtaataat tcctgtacta aaaggcataa
     1621 ttgctttaat tattttttga cttctcttca aacacaagaa gtagttgtgt aataattgca
     1681 gaaataagga ttccaataat agatataaaa tttgacatgg aaacgtctat aacattaaaa
     1741 gaataaagtg ttgaagaaag taaactgatt gaaataaccc ctccgtaaaa aatagtctgt
     1801 gaatataccc actttttttc agaagacgta cttcgtttgg ttctaaaacc tatccctgca
     1861 tttctactct tatcaaaaaa ccaaatattt agaagtaata atattgctat tattaagata
     1921 aaattactgt aaaaatccaa aagctatc
//
LOCUS       DASJON010000294         1873 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.301, whole genome shotgun sequence.
ACCESSION   DASJON010000294 DASJON010000000
VERSION     DASJON010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.301"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..344
                     /locus_tag="U3989_002837"
     CDS             <1..344
                     /locus_tag="U3989_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN1716245.1"
                     /translation="TGRYLIIGGLIAAAMQTYLPTRIMLTLGSTKILAIIIMLLLAFT
                     MSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDIKNLLMMKRYFTGRFILSLIGLI
                     ALSVIGFTLFI"
     gene            355..1191
                     /locus_tag="U3989_002838"
     CDS             355..1191
                     /locus_tag="U3989_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN1716246.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            1439..>1873
                     /locus_tag="U3989_002839"
     CDS             1439..>1873
                     /locus_tag="U3989_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1716247.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKR"
BASE COUNT          609 a          296 c          371 g          597 t
ORIGIN      
        1 atacaggtcg ttatttgatc attggcggat taatcgcagc tgcgatgcag acttatttac
       61 caactagaat tatgctaacg ctcggctcaa caaagatttt agccattatc atcatgctgt
      121 tattggcctt tacgatgtct ctttgctcag aagcagatgc gtttatagga tcttctttgt
      181 taagtctgtt cggcacagca cctgtagctg cctttttggt ctttggccca atggtcgata
      241 tcaaaaatct actgatgatg aaacgatatt ttacaggacg ctttatttta tcgctgatcg
      301 gactgattgc ccttagcgtt attggattca cattatttat ttaggaggga aaatatgctg
      361 cggtttttga ttttatctgg gtatttcgaa ttgatgatgt acttgcaagt ttctggcaaa
      421 ctcgatcaat atatcaatgt ccattatcgc tacttagcta ttctttcaat gattttatct
      481 gctcttttgg ctttagttca gctgtatttg tgggtcaaaa acggaaaagc agaagaaaag
      541 gcacacgagc acgatcatga ccatgggttg tcaaaacctt atcaacgtgg aatcgcttat
      601 gtactgctag ctttgccagt aatcgtgggg acgttgtttc caacagttag tctcgacact
      661 acgatcgtag aagccaaagg atttaatttc ccagtaagta aagaatcagt aggggatccg
      721 gagatgcaga cacaatactt aaagccggat accagtattt atttcaacaa atcagactac
      781 ttagaccaaa tgaatgagtt gaaagaaaag tatggggata agcaaactat caagatcaca
      841 gatgaaaact atttagaagt tatggagcta atttataatt accctagcga attcattggc
      901 aaaaaaatca gctatgaagg atttgtttac caaacaccga acgatagcaa tacagatatc
      961 ttcttgttcc gattcggcat tatccattgt gtggcagatt caggagtctt tggtttgttg
     1021 acgcatatgc cggaaggagt aacggttaaa aatgatgaat ggtataaaat agaaggaacc
     1081 atccaatcag actattatca accattcaag cgtgaaatcc catctgtagt tgttacaaac
     1141 gctgagaaag tctctgctcc gaaaaatcag tatgtgtatc gttcttttta aaagaacaaa
     1201 ataaataaac atagctcaga cagctgacac gcaaggacgt cggttgtctg gctttttttg
     1261 catgatgtat tcatttgttt gatatttatt ttagaagcag catcctgaaa gtatttgata
     1321 tcggtttcaa aggtgttgga aaagattgtg aagagaatta caatttattc ctaacatgta
     1381 tgttgaaaag ttgtatgaaa tgattttgat agtaaaatat cgagtggagg tactttttat
     1441 ggtaacgcgt atatttattt tggatgatag tttggttttt gaaaaaatgc tagtagaaat
     1501 tttggaagaa tccagaattt tattaagtcc ttggaaattt gagatcgttc agggattgaa
     1561 cgttatacaa tttgttgaat tcattaaaaa taatgaaatc tattcctcag atattttttt
     1621 cctagacatc gatttgaacg cctattatag cggtatcgaa ctaggcgaaa tgcttagaga
     1681 aaagaatgaa aagtgtaaaa ttgtttatct aacgtctcat ccgaataaag caataactgt
     1741 tattaatcga aagattacgc ctagtggtta tttgataaaa gaattcactt atgaaaaaaa
     1801 taaaaaaagc atccaaagtt acttattgaa agaaaaagaa aactatcctg ttcaacatgc
     1861 tacgaaaaaa agg
//
LOCUS       DASJON010000295         1870 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.302, whole genome shotgun sequence.
ACCESSION   DASJON010000295 DASJON010000000
VERSION     DASJON010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.302"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            286..1770
                     /gene="guaB"
                     /locus_tag="U3989_002840"
     CDS             286..1770
                     /gene="guaB"
                     /locus_tag="U3989_002840"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN1716248.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          639 a          333 c          424 g          474 t
ORIGIN      
        1 actaagcagc tttttcacag cctctccact aaacggtgtt caaaagtttg catctgcggt
       61 aataaaccca accaattcaa aaacatgaga agctgttttt gattggctgt tcttattact
      121 tgatgcagga cagctttttt acaatctctt ttaatacaca cagatgtaaa cgctttatat
      181 tggtattcta tgagaaagct aacttgattc caagtagatc attgacttaa atgaaaaaat
      241 caattatttt aatagataaa ctaaattaga ggagtggtac taaaaatgtc caactgggaa
      301 acaaagttca cgaaaaaagg atttactttt gatgatgtct tattgatacc agcagaaagt
      361 cacgtgttac caaacgaagt ggatatgagc gtacaattag ctaaaaatat taagttgaat
      421 attccaatca tcagtgcaag tatggatact gttaccgaca gcaagatggc cattgctatg
      481 gcaagacaag gcggactagg tgtgatccat aaaaacatga cgatcagcca gcaagcggat
      541 gaagtaagaa aagtcaaacg ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg
      601 ccgcagcatc ttgtagcaga cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg
      661 ccaatcgttg aaacacttga aaacagaaaa ttggtaggga taattacaaa ccgcgacatg
      721 cgttttgtaa cagattatca catgccgatt gctgatgtga tgacaaaaga caacttagtg
      781 actgccccag taggtacctc tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc
      841 gaaaaattac caatcgtaga taacgaaggt cgtttaagcg gattgatcac gatcaaagac
      901 atcgagaaag tgatcgaatt tccaaatgca gcaaaagatg aacatggtcg attgcttgta
      961 gcagcagccg ttggtgtaac aagcgataca tttgagcgtg cgaatgcttt attagaagca
     1021 ggggcagatg caatcatcat cgatacagct catggacata gtgcaggtgt gatccgaaaa
     1081 attcaagaaa tccgttcaac ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg
     1141 gctgaagcga caaaagcttt atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga
     1201 ccaggatcga tttgtacgac acgtgtggta gccggtgtag gtgtacctca gctcacagct
     1261 atttatgatg cagcatcagt tgctcgtcaa tatggcaaag cgattattgc agacggaggg
     1321 atcaaatatt ctggtgacat cgtcaaagca ttagctgccg gcggacatgc agtgatgcta
     1381 ggaagtatgt tggcagggac agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt
     1441 cgattcaaaa cataccgagg tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac
     1501 cgttacttcc aaggtagtgt caatgaagca aataaattag taccagaagg aatcgaaggt
     1561 cgggttgctt ataaaggaag tgttgcagat attattttcc aaatgatcgg cggtttgcgt
     1621 tcaggaatgg gttatgttgg ggcagccaat ctacagcaat tacgtgatga agcgcaattt
     1681 atccaaatga gcggcagtgg attaaaagaa tcacaccctc atgatgtaca aatcacaaaa
     1741 gaagcaccaa actattcagt tgaatcttaa aaacattaaa gagcgtggga caaaagtaaa
     1801 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagtagaag caactccttc
     1861 ggaaataaga
//
LOCUS       DASJON010000296         1859 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.303, whole genome shotgun sequence.
ACCESSION   DASJON010000296 DASJON010000000
VERSION     DASJON010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.303"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="U3989_002841"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="U3989_002841"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1716249.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DASJON010000297         1852 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.304, whole genome shotgun sequence.
ACCESSION   DASJON010000297 DASJON010000000
VERSION     DASJON010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.304"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            116..988
                     /locus_tag="U3989_002842"
     CDS             116..988
                     /locus_tag="U3989_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1716250.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            1070..>1852
                     /locus_tag="U3989_002843"
     CDS             1070..>1852
                     /locus_tag="U3989_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1716251.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKI"
BASE COUNT          643 a          269 c          370 g          570 t
ORIGIN      
        1 ttccagacta ccaagacgaa attttcattc cgaaacttga agaagtaaaa caaggacaag
       61 aattgtttgt tcttggctaa tgataagtaa ttaataatag aatcgaggaa aatgtatggt
      121 ttttggggct attgaagctg gtggaacaaa atttgtttgt gccattggag ataaagattt
      181 gaacatattt gaaaaagtca gctttcccac gactacaccc aaagaaacaa tgaatttagt
      241 tttagatttt tttaatcatc ataaagatga tttagaaggt atcggagtgg gatcgttcgg
      301 tcctattgat attcattgtg aatctaaaac gtatggcttc attacatcaa cgccaaaact
      361 tgcttggcag gattttgatt ttattggaag tttaaaaaag caacttaata ttccaactgc
      421 ttggacgact gatgtaaatg cctcttgcta tggtgaatat gtttctggaa atggcagagg
      481 cctttcaagt gttgtttact ataccgtagg aacgggcata ggaggtggcg ctatccaaaa
      541 cggaaacttc attgaaggtt ttagtcatcc agaaatggga catatgcttg taaaacgaca
      601 caataaagac aacttttctg ggacatgtcc attccacgga gactgtttag agggcttagc
      661 tgctggtccg gcaatcgaaa gaagatcagg gaaaaaggga caagaagtga ctgaaaatga
      721 cgttacttgg gaaatagaag cttattacat tgctcagtgt gcatataata caacattgat
      781 gttatcacct gatgtcatta tatttggtgg cggagtaatg aaacaaaaac acttaaaaaa
      841 gaaagtacaa gataagttta ttgagttgat caacggatat gtcgctattc ccgatgttga
      901 caattatatt atgacaccaa aattgttaga taattctggt attgtaggtt gtttagcttt
      961 agcaagagac gttaaagaaa aatcttaatt tggaaaaaag atcgtctcga ctatttgttt
     1021 ggttgatgcg atcttttttt tcaattagta attgaaggga cgcctaaaag tgaaaaaaaa
     1081 aatgaaagat gaagtattag agtatatcaa aaataattta aagtattacg atttttctgc
     1141 tcaagatatt gcaatgaaat tttgtattaa aagaaatgtc gcttcccact atttgaatca
     1201 gttattttca gatggaaaat tactaaagaa tgattctgtt agaccagtaa tgttcaagta
     1261 taatcaacaa aagccaaaag actgcttttc taagtttatt ggagcagaca tatctttgaa
     1321 atccactata gataaatgca aagctactgt aatgtatccc cctaatggcc tcccattaat
     1381 aataaaagga aacagtggcg ttggaaaaag ttttttagca agtttgatct atcagtatgc
     1441 tcttgataga aaagtaattc ataatgacgc aaaatttgtt gttgtgaatt gtgcagatta
     1501 tgcaaataat ccagagctat tgtcagcagt tttatttgga tataaaaaag gagcctttac
     1561 tggggctgaa aaagatactg cagggatgct atcttctgct aatggaggat accttttttt
     1621 agatgaggtt cacaatctaa gtgcagaaaa ccaagagaaa ttgtttcttc ttatggattc
     1681 tcaaaagtat cgtaaacttg gtgaaagtgt gcaatgggag tatgcaaatg ttcgccttat
     1741 cttagccaca acagaagata aaaatacctc tttgttagca acttttagaa gacgcatccc
     1801 agctgaaatc actctgccag attacaataa tagaagtaca tctgaaaaaa tt
//
LOCUS       DASJON010000298         1817 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.305, whole genome shotgun sequence.
ACCESSION   DASJON010000298 DASJON010000000
VERSION     DASJON010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.305"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(485..1057)
                     /locus_tag="U3989_002844"
     CDS             complement(485..1057)
                     /locus_tag="U3989_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1716252.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1073..1678)
                     /locus_tag="U3989_002845"
     CDS             complement(1073..1678)
                     /locus_tag="U3989_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN1716253.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          535 a          367 c          270 g          645 t
ORIGIN      
        1 aaggagactt gaatgccatt attactttaa tcgtagttat ggtaaaatca ataggtaata
       61 tcacaatagg aatgctgttt attatattag cagcagtgct gatttttatg atagttattt
      121 ggttcttaaa tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa
      181 tagagtcaat agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac
      241 ttacgctgaa cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga
      301 tggtatccta atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt
      361 accatacaca ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat
      421 gctaatagac tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc
      481 ttttttaact ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg
      541 ttatagcttc aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct
      601 tcttcggtgt gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg
      661 caaacatttt tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac
      721 tcaacaacgt ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca
      781 aaaccttgat cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg
      841 tgttacgagc aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag
      901 ccaacagctg attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa
      961 tattcttctt ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag
     1021 aagcggttga gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat
     1081 catctatcgt atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat
     1141 ataactcgcg atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca
     1201 aatcgtttat gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc
     1261 aatctacaac tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc
     1321 ggccatttcc ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct
     1381 cttcaaaaga agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa
     1441 ctggtgcaaa tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat
     1501 cgtaaatatc cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt
     1561 cgatttcatt tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt
     1621 cttcaacctt agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc
     1681 gaaatctcct taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg
     1741 attttgttta tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc
     1801 tctattttgc acagtct
//
LOCUS       DASJON010000299         1812 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.306, whole genome shotgun sequence.
ACCESSION   DASJON010000299 DASJON010000000
VERSION     DASJON010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.306"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1170
                     /locus_tag="U3989_002846"
     CDS             <1..1170
                     /locus_tag="U3989_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN1716254.1"
                     /translation="AVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDM
                     VAIGNGTASRESELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEER
                     SAVSIARRLQDPLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVN
                     TASPQLLQHISGLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVP
                     GGKNILDNTGIHPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGE
                     ETLADILAGLTQPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDI
                     GVKQDGLVHISKLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            1190..1672
                     /locus_tag="U3989_002847"
     CDS             1190..1672
                     /locus_tag="U3989_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN1716255.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            1688..>1812
                     /locus_tag="U3989_002848"
     CDS             1688..>1812
                     /locus_tag="U3989_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1716256.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCL"
BASE COUNT          622 a          338 c          410 g          442 t
ORIGIN      
        1 gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag ttatttatcc tcataaacct
       61 gcagcgcaag caaaaagaga agctgctgga ccagctttta tccaattaat taataaatat
      121 caagtagata tggtagctat cggaaatggt acagccagtc gggaatctga actttttgtg
      181 gctgagcagt tgaaatccgc cgaccataaa acctattacg ctatcgtcaa cgaagcaggc
      241 gcttctgttt actccgctag cgaaattgca cgaaaagaat ttccgcatct acaagttgaa
      301 gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc ctttagctga attagtcaaa
      361 atcgatccta aagctgtcgg agtaggccag taccaacatg acgtttctca aaaacgtttg
      421 gctgaacaat tggattttgt tgtggaaaca gcggttaacc aagtaggtgt ggatgtgaat
      481 acagcaagcc cgcagctctt gcagcatata tcaggattga ataaaacaac tgcccaaaac
      541 attgtcattt atcgtgaaga aaacggagaa ttcaccgcac gaactcaatt gaaaaaagtg
      601 ccacgcttag gaccaaaagc atatgaacaa gcaatcggat tcttgcgagt ccctggtggg
      661 aaaaacattt tggacaacac aggtatccat cccgaaagtt attctattgc aaaagaaata
      721 ttaacttcag tccaactttc agaaaaagag cttggtacag aagaagcagt agaaaaattg
      781 acacgattat ccgtagagaa actggctgaa agcctatcag tcggagaaga aacgttagcc
      841 gatattttag ctgggctgac acaacctgga cgagatatgc gtgatgagat gccagcgccc
      901 ttattacgta cagatgtatt gagtatggaa gacttgaaac cagggatgga attgactgga
      961 acggtccgta atgtcattga cttcggcgca tttgttgata tcggtgtgaa gcaagacgga
     1021 cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc atcctacaga tgtcgtgtca
     1081 gtaggagata tcgtaacagt ttggatagaa caagtcgata cgaaaaaagg cagaatcagt
     1141 ttgacgatgc tttcccctta tgaagagtaa caatgaggag taacaaaaat tgacacaaga
     1201 agaactgcag cagttagtag aagagatatc attaaaaagc ttccagcttc ctttcaagca
     1261 ccaagcaatg ttcaatccac gcttacgtac aactggcggg cgctatcatt taaacgatca
     1321 tcatttagat tttaatgcga agctatttga agagtcagac gaagagacga ttgcaggaat
     1381 catcaaacat gaactttgcc attatcatct tcatctagct ggaagagggt atcagcataa
     1441 agatatggat ttcaagtatc ttttgaaaaa aacaggaggg ttgcgctatg caccagcctt
     1501 gacgaagaaa cagataaaga tggagtatta tcgatgccaa gattgttctg ccacgatctg
     1561 ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga agatgtcatg gagaaatcaa
     1621 atggattgag acaaaaagag aagactttaa tgaagcacaa aaaatcagat gaaaggatag
     1681 aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac tggaatcagc tattaaattt
     1741 tttagccgca cctgaagagg gaaattttga ggaactgacg gcacctacac ttattttagc
     1801 tggaaactgt ct
//
LOCUS       DASJON010000300         1781 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.307, whole genome shotgun sequence.
ACCESSION   DASJON010000300 DASJON010000000
VERSION     DASJON010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.307"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..285)
                     /locus_tag="U3989_002849"
     CDS             complement(<1..285)
                     /locus_tag="U3989_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1716257.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMT"
     gene            429..1202
                     /locus_tag="U3989_002850"
     CDS             429..1202
                     /locus_tag="U3989_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1716258.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1373..1739
                     /gene="ssrA"
                     /locus_tag="U3989_002851"
     tmRNA           1373..1739
                     /gene="ssrA"
                     /locus_tag="U3989_002851"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          571 a          337 c          337 g          536 t
ORIGIN      
        1 tgtcataata aaaataggca taattggaaa tactagcaga agcatttcaa tcagaaaata
       61 attcgtatgc ccagatgtct ttaaataata gccgatcaat gccatcagta agcccataag
      121 aaaaaaccag tttccgcttg tcttttgagc cagccgccaa tgagcgtctg tttttgcggc
      181 aagaggcgaa tgatacccat aaaaaggcag atttcctttt gagggcagaa tccgaaaaag
      241 actgccaatt attaagaata agataccaat actgaaaaaa atcattcttt ccctcctaaa
      301 ttctcttctt atagtatact ctaaattgtt gactgaacca atatgttcag gaattcttca
      361 cgaaaggttt gtcttttgtt cattctttgt taagataaac ttattccaat aaaaaaggag
      421 ttttatttgt gtcaaattat cgtgcaatca ctttctttga tcttgatgga acattactag
      481 atgcgaaatc aaaaatcaca cccgaagtgg caaaagctat gaacgcatta aaagaaaaca
      541 atgttctccc cgtcatcgct acaggacgta cagaagcaga aatccactct atcatggaag
      601 atgcgggcat tacttctgcg gtcaccatga atggttcttt cattcgagta gacggcgaag
      661 aaatctattc tcaaacattt tcttcagaag aatgtcaaaa aatgtatgat catgtacagt
      721 tgaacggtca tgaactttct tactataacg atcagaaaat ctggtgtacc ggtcatagcc
      781 aaacaatgat caatgcttac gacttcattc attcagatgt tccggaaatt gatcctttag
      841 gctttaaaga aaacacagta aatatgttgc tgatcttatc tcaatcaggt gacgaatact
      901 atcacgaaca ttttcctgag ctaacttttt atcggaatgg tccttactcg atcgatacag
      961 tgaagaaagg aatctcaaaa ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg
     1021 aaaaagttcc tacttttgga tttggcgatg gtccgaatga ttttgccttg ttggaagctt
     1081 gcgacaacaa aatcgctatg ggcaacgcct atgacggatt aaaagaactt tctaccttta
     1141 tcacaaagaa aaatacagaa ggcggaattg tccacgcatt gaagtatttt gatctgattt
     1201 aagatctttt tttgaagcta cctcttttct taagaaaatc gcttattttt tcttaagaaa
     1261 ggggcagtta ttatataaat aatgggatat gattcgcttt tttattgaat ttatcccaat
     1321 atatgctata ctaattttgt atgaaaaata cacgtaaccc ttgttagcta acggggacgt
     1381 tacggattcg acaggcacag tcgagcttga attgcgtttc gtaggttacg tctacgtaaa
     1441 aacgttacag ttaaatataa ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta
     1501 aaaacagtta gcgtagatcc tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt
     1561 tagtgggata cgtttcaact ttccgtctgt aagttgaaaa agagaacatc agactagcga
     1621 tacagaatgc ctgtcactcg gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa
     1681 cgtagatttt taagtggcga tgtgtttgga cgcgggttcg actcccgccg tctccattgt
     1741 gagatttcag cagatttcgc tagatgctta aatccttatt t
//
LOCUS       DASJON010000301         1772 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.308, whole genome shotgun sequence.
ACCESSION   DASJON010000301 DASJON010000000
VERSION     DASJON010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.308"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..737)
                     /locus_tag="U3989_002852"
     CDS             complement(<1..737)
                     /locus_tag="U3989_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN1716259.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVI"
     gene            1131..1754
                     /locus_tag="U3989_002853"
     CDS             1131..1754
                     /locus_tag="U3989_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1716260.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          587 a          299 c          330 g          556 t
ORIGIN      
        1 tcaatcacga ctttcgcatt tggcagaaca cgtttggtga gctggaagta ggcggcgttc
       61 atgtctgtca ccaagaattc tacttcttct ggattggtac aacctaagaa atagcttgtt
      121 aatcgaggta atttacgcgt aggcaaaaca tctattaatt ttcctgtttc gccatccgcg
      181 caaataaagc tcatcttatc ttctatggaa gcatgcgaac gaaattcgtc aaccatcaat
      241 actctgggga ggatcttctt agattgcttt ggtaaatagc ttttaaactc tttcaatgta
      301 cgaataacgg tggtcaaaga tacctgacag cttttcgcaa taaaagataa agatactttt
      361 tcagtcagta aagaagcaat tttatatcta acatgatttg cgattgaatg tctgggttgg
      421 acaaaataac tttgagccgt ccaatgggtg cgacagtttt tacaggtata gcgttgcttt
      481 tttaggcgca taaccaaagg catatgattg tattgttcaa aacggacaat cgtttccttt
      541 tttccatttt tcactataat tgctttcccg tttccatcta ccacagtaga accacaactt
      601 ctacaagcac gaggagtagg cgagagaaca gcatcgacga ccaacgtctt tttcttctga
      661 agggtctcgt aagagacctc tgtaatcatc aaatctttct ctgttattct cagcattttt
      721 ttgatagaat cattcatata gcgtatcgtc ctctcagttg tttattttgt ggtgatttaa
      781 tcatactaga gaacgatatg ttttttaata cctaaaatga aaatggggct gaagaatcaa
      841 ttctgattca tcagtcccat aaattataga gccaaaataa actaatgtcc ttatttttgt
      901 gttcacattt ctgttaccta agaaatactt aaaggaggtg atgtagccag caacgactac
      961 aacttgaacc cgctatctac agtttatttg ttgtttcttt tgggtatact ctaatggtac
     1021 aaatccatat ttgcttaaag cgttgatttt tcagcggttt ttctttattg caaagtgtac
     1081 cctaagacct gtatactaat agtaaataga ttggatagga atgatggcaa atgtatttag
     1141 cttatttaag agtttcttcc gacgatcaaa gtttagctcg gcaacataag ctaattgaaa
     1201 attggtcagt caaaaataat attgctgaag acgatttaaa aattttcgag gaaaaagtct
     1261 ctggaaaaaa tattgaaaac agaccagagc tgcaagagct tatttctttc atacgtgaac
     1321 gagatacgat tattattcct agtcttgatc gattagcaag aaatagtaaa gatacaaaag
     1381 acttacttca attgtttcgt agtaaaggtg cttctattga aatattagac ctaccgtcgt
     1441 ttaatggggt aactgaccct gcattaagag acttgctaac aaatttagtc atagaagtgt
     1501 ttagctatgt tgctgaaaac gaacggaaaa aaatcaaaga gcgtcaaaaa caaggcattg
     1561 aaattgcaaa gaaaaatggt cgttatactg gtaaaaagat tcaataccat gccaatgcaa
     1621 caggaaaaaa caaattgatt tacgatgaaa ttgttgatgg tctgaagaaa aaagaaacta
     1681 tttctaaaat agcaaagaac gcaggagtta caagaaaaac cgtttatcga gtaaaaagag
     1741 atttaggaat gtaatatgtt ccagttagaa ag
//
LOCUS       DASJON010000302         1761 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.309, whole genome shotgun sequence.
ACCESSION   DASJON010000302 DASJON010000000
VERSION     DASJON010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.309"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1016..1357)
                     /locus_tag="U3989_002854"
     CDS             complement(1016..1357)
                     /locus_tag="U3989_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1716261.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="U3989_002855"
     CDS             complement(1357..1587)
                     /locus_tag="U3989_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1716262.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          593 a          286 c          241 g          641 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat t
//
LOCUS       DASJON010000303         1747 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.310, whole genome shotgun sequence.
ACCESSION   DASJON010000303 DASJON010000000
VERSION     DASJON010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.310"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..>1747
                     /gene="copB"
                     /locus_tag="U3989_002856"
     CDS             33..>1747
                     /gene="copB"
                     /locus_tag="U3989_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN1716263.1"
                     /translation="MHMGHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWL
                     SLILAIPIILFSPMMGMEFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQK
                     SPAMMTLIAMGITVAYIYSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNA
                     VSNASNALQKLAELLPESVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILK
                     GETIVDESAVTGESRGIKKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRK
                     AQGEKSKLESLSDKVAKWLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHA
                     LGLAIPLVVARSTSIAAKNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEV
                     LDDAFNKNEILQYLGALEANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATV
                     KNQQVKIVNEKEVERLGLNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEA
                     KEFIRTLKERKITPVMLTGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGK
                     KVIMVG"
BASE COUNT          595 a          309 c          376 g          467 t
ORIGIN      
        1 tgaaacatga ccatagccag atggaccatt ccatgcacat gggacataac catggagata
       61 tagcaaacag caaacaaatg gaccattcga tgcacatgga tcacgatcat ggcggcatgg
      121 atcattccat gcacatggga aatttcaaac aaaaattttg gctatcgctt atcctagcta
      181 tcccaatcat tcttttttca ccaatgatgg gaatggaatt tccatttcaa gtaacgtttc
      241 ctggttctga ttggctcgta ttgatcttag caactatact atttatctat ggcggtcagc
      301 catttttaag tggagctaag atggaattga aacaaaaaag tcctgctatg atgacactga
      361 tcgcgatggg gattacagtt gcatatattt atagtgtcta ttcgtttatc gcaaatctgc
      421 tcaatccaca tacacacgtc atggatttct tctgggaatt ggctactttg atcgttatta
      481 tgctactagg tcactggatt gaaatgaatg ctgtgtcgaa tgccagcaat gctttacaaa
      541 aactagcaga attactgcca gaatcagtaa aacgcttgac gaaagatgga aaagaagaga
      601 cagtttcatt gaaagaagta aacgaaggtg atcgtttgat cgtccggtct ggtgataaaa
      661 tgccaacaga tggtgtaatc ttgaaaggtg aaacaattgt tgacgaatca gctgtgactg
      721 gcgaatcaag aggaatcaaa aaacaagcca atgacaaagt gattggcggt tctatcaatg
      781 gggatggaac gatcgaaatc gaagtaactg gaactggtga aaatggttat ctagcgaagg
      841 ttatggaaat ggtgcgaaaa gcacaaggag aaaaatccaa gctagagtca ttatctgaca
      901 aagtggcaaa atggctgttt tatgtcgcat taattgtggg tatcctggca ttcatcgcct
      961 ggttgttctt gacagatctt cctaatgcat tagaacgtat ggtaacagtc tttatcatcg
     1021 cctgtcctca tgctctaggt ttagctatcc cattagttgt tgctcgctct acatcaatag
     1081 cagctaaaaa cggattactt ttgaaaaacc gcaatgcact ggagcaagcc aatgatctag
     1141 atgtgatcat gctggacaaa acaggaacac taacagaagg aaaatttact gtaacgggtg
     1201 tcgaagtact tgatgatgcg ttcaataaaa atgaaatcct tcaatattta ggagcattgg
     1261 aagcgaatgc caatcatcca ttggctgttg gtattatgaa ttatttgaaa gaacatgaaa
     1321 tcaagcctta tcaagccgaa aatctaaaaa atctttcagg agttgggcta gaggcaacag
     1381 tcaaaaacca acaagtgaaa atagttaatg aaaaagaagt tgagcgtctc ggattaaacg
     1441 tagaacaagc attattgaaa ccttaccaag aacaaggaaa taccatcagc ttcctgattt
     1501 tagaaaatca tctagctgca atcgttgctt taggtgacgt agtaaaaaca gaagcaaaag
     1561 aatttatccg aacattgaaa gaaagaaaaa tcactcctgt catgcttact ggtgacaata
     1621 aaaacgctgc ccaagcagta gcagattatt tagggatcga agaatattat ggcggtttat
     1681 taccagatga taaagaagct gttgtccaga aataccttga ccaagggaaa aaagtaatca
     1741 tggtggg
//
LOCUS       DASJON010000304         1742 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.311, whole genome shotgun sequence.
ACCESSION   DASJON010000304 DASJON010000000
VERSION     DASJON010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.311"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..403
                     /locus_tag="U3989_002857"
     CDS             5..403
                     /locus_tag="U3989_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1716264.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="U3989_002858"
     CDS             387..1082
                     /locus_tag="U3989_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEN1716265.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          648 a          254 c          336 g          504 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 gg
//
LOCUS       DASJON010000305         1736 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.312, whole genome shotgun sequence.
ACCESSION   DASJON010000305 DASJON010000000
VERSION     DASJON010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.312"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(192..1484)
                     /locus_tag="U3989_002859"
     CDS             complement(192..1484)
                     /locus_tag="U3989_002859"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN1716266.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          498 a          366 c          303 g          569 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaataagaa ggaatgtacg
     1681 aaaattgctt ctcaaatttt tgtgaaattt cgacttattt ttcgaagaag ctactt
//
LOCUS       DASJON010000306         1691 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.313, whole genome shotgun sequence.
ACCESSION   DASJON010000306 DASJON010000000
VERSION     DASJON010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.313"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..385)
                     /gene="zapA"
                     /locus_tag="U3989_002860"
     CDS             complement(<1..385)
                     /gene="zapA"
                     /locus_tag="U3989_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN1716267.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQ"
     gene            587..1504
                     /locus_tag="U3989_002861"
     CDS             587..1504
                     /locus_tag="U3989_002861"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN1716268.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
BASE COUNT          529 a          337 c          302 g          523 t
ORIGIN      
        1 gttgtgcttc cacatgattt ttaatggtat gatctgcttg accattttct ttcaatactt
       61 cccgagcttc gttctcgatt gcttcaatgc gtttcacacg attttcaagc tctgtaaatt
      121 tgatcatttt tttctttaat tctgctgttt cttcttccag ctccaatatt ctttcttgtt
      181 tcttcaattg atccgaaagc gcattgacag ccatcaagat tgctgcttgt tcattatcca
      241 tttgtggtga taattgtttc aattctgcta actgttcatt gatcagcttc gttacgatat
      301 ccatatgatg cttggtttcc cgtccgataa tagtatatgt ttggttggca atcaccgctt
      361 tgtagcgtgt tttttcgtgc gccatacgaa tcatttcctc ctatttgatg aaagttttat
      421 ttagtgtacc atgaagttgg ttctttcacc gttctccaca aatatttttc acatacttaa
      481 ctattatatg ataaacttta aaaaaatgct atgtctcgca atctcttaga aaacgaaaag
      541 tcgtgagttg cggtatagtt ttatgtataa aaacgaggag aattttatga gcaacgtcat
      601 tatcaaagca acaaacgacc aacttagtca aatgaaagca tactatcgct cttatttgtt
      661 aaataaacag attccttata ctagctttgc tgcaaaaaaa gacgggacga ccattaccgc
      721 ttatacatcc ggaaaagtga tgtttcaagg tacgaatgca gcaatagaag ctgataaatg
      781 gggggaggca caggataaac aagccaagcc agcaaaaaca gcctcttctt tgccaaaaaa
      841 tatcagtcaa ttatccgttt taggtagtga tgaggtcgga aatggcagct atttcggtcc
      901 gctaacagta tgtgctgctt atgtgaaacg tgatcagctt gcagaattac gtaaacttgg
      961 tgttagagat tcaaaagagt taaaagatag ccaaatcatc cagcttgctg acatcttaaa
     1021 aaaaaccatc ccctataaac tgcttgtctt atctccagaa aaatacaatg agatccaacc
     1081 aaaatacaat gccgttcgga tgaaagtcgc tttgcacaat caagcgatcc atttgctgct
     1141 aaaaaaaatc gagccagaaa aaccagaagc gattttgatc gatcagttta cgccggaagc
     1201 aaattttaaa aaatatgcgc gtttagaaaa aaatcaatta caagaaaaac tgtacttcgt
     1261 tactaaagga gaacaatatc atttagctgt tgctgctgct tctatcatca gtcgtgccag
     1321 ctttttggaa gagttagaca aagcatcagc tgaagctgga atcacccttc cgtcaggagc
     1381 tggaacgaaa tctgaccaag tagcggcaaa actattggag aaaggcggca tgcctatgct
     1441 tgaaaaatat gccaaacttc attttgccaa tacagaaaaa gctttgaagc tactaaaaaa
     1501 ataatggaac tgtgacaaaa gtcatgtcac agtcccttgt tccgaataaa cggtgttcaa
     1561 tcatctgatc ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt
     1621 tgtttctcta atactcagaa caaaacgttg atttcacaac ctttttctta aacggtgttc
     1681 tcatgccaac t
//
LOCUS       DASJON010000307         1672 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.314, whole genome shotgun sequence.
ACCESSION   DASJON010000307 DASJON010000000
VERSION     DASJON010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.314"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="U3989_002862"
     CDS             complement(<1..182)
                     /locus_tag="U3989_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1716269.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="U3989_002863"
     CDS             517..951
                     /locus_tag="U3989_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1716270.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="U3989_002864"
     CDS             974..1294
                     /locus_tag="U3989_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716271.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="U3989_002865"
     CDS             1435..1626
                     /locus_tag="U3989_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716272.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          634 a          252 c          356 g          430 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gt
//
LOCUS       DASJON010000308         1664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.315, whole genome shotgun sequence.
ACCESSION   DASJON010000308 DASJON010000000
VERSION     DASJON010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.315"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U3989_002866"
     CDS             131..505
                     /locus_tag="U3989_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1716273.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U3989_002867"
     CDS             complement(619..1248)
                     /locus_tag="U3989_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1716274.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U3989_002868"
     tRNA            complement(1382..1454)
                     /locus_tag="U3989_002868"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DASJON010000309         1653 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.316, whole genome shotgun sequence.
ACCESSION   DASJON010000309 DASJON010000000
VERSION     DASJON010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.316"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            138..1604
                     /locus_tag="U3989_002869"
     CDS             138..1604
                     /locus_tag="U3989_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN1716275.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          557 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcctgt ttt
//
LOCUS       DASJON010000310         1646 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.317, whole genome shotgun sequence.
ACCESSION   DASJON010000310 DASJON010000000
VERSION     DASJON010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.317"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..507)
                     /locus_tag="U3989_002870"
     CDS             complement(<1..507)
                     /locus_tag="U3989_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN1716276.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNL"
     gene            1201..1587
                     /locus_tag="U3989_002871"
     CDS             1201..1587
                     /locus_tag="U3989_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1716277.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT          607 a          259 c          203 g          577 t
ORIGIN      
        1 cagattatct attttgattt gctcgatagt tttacttaga tttcctgtat attcaattaa
       61 cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta aaatcagaat
      121 atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat agagtcgttc
      181 tctatcagcc attgcaacat tcaaattgtt catttttagt ttaaaaaaat ctttatcaat
      241 attatttgct ctaaatggac caaataagta atatctggtt ttaaacatgt atagaagaaa
      301 catctcatta aaatcaccag taaagaaatg gaaatcttta gtttttttaa tattatcagt
      361 tagtaacttt ttataatcat aaaaaaaata agtagttcta tctgagcgat actcttttat
      421 aatactgaat tcggaattaa aaaccacgat aggtaacttt aaacaaacat gaagagactt
      481 taataagact tctttatctg aaaacacttt tgtttctctc tttcctttct acatcattaa
      541 atagtatttt atacgttatc taaaatacta ttcctctgtc aaaataagct aacttacttc
      601 attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt acattttatc
      661 accgagaact catcattttt catcatgatg aatactattt acatatatct agttcaaaga
      721 caataattat tatgatccgt tattatctaa gcactataca gataatagga atttttcgtt
      781 tctaaatact ccaatttggg aatccaataa aaattttcgt tcaaatacgt gtctattcga
      841 actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat ctatacacga
      901 ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa aaagctcaag
      961 acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa ttttcttttc
     1021 taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa taaacatcag
     1081 cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat aattgcatta
     1141 gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga ggtgtgcaat
     1201 atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga aaaggtcatt
     1261 ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca tgaacaactt
     1321 gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca accgattaat
     1381 gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct tcctttgttg
     1441 aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttaataaaac tgataaagaa
     1501 attggaaagg ttctgggtat tacaagacag ggtgcgacaa atttaaaaca tcgactttac
     1561 aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg tatgcttctt
     1621 tgctcgtcct cctccttccc caaaaa
//
LOCUS       DASJON010000311         1636 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.318, whole genome shotgun sequence.
ACCESSION   DASJON010000311 DASJON010000000
VERSION     DASJON010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.318"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(183..1502)
                     /locus_tag="U3989_002872"
     CDS             complement(183..1502)
                     /locus_tag="U3989_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN1716278.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DASJON010000312         1625 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.319, whole genome shotgun sequence.
ACCESSION   DASJON010000312 DASJON010000000
VERSION     DASJON010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.319"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(312..1625)
                     /locus_tag="U3989_002873"
     CDS             complement(312..1625)
                     /locus_tag="U3989_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1716279.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT          546 a          359 c          281 g          439 t
ORIGIN      
        1 atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta aaaattgtaa
       61 cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa aaaacagctg
      121 aggaaattcc ctcaactgtt ttttgaaagg attgatttat gcagggaaga atgctgattt
      181 cacaacttct ttataaacgg tgttcactca gctgtccctt ggtattagct caaaaaacgg
      241 caaaatataa gaaactattt tttcgttttt ttctctaata ctcaggccag aacgctgatt
      301 tcacaacctc tttatttcac attcaatatc cacatgaatc ccacaaatac aaagaacaga
      361 ccatacatga ttggagatac ttcttttcct cttttagctg ctaacatggt aattggatag
      421 aagataaaac ctaatgcaat cccatctgaa atactataag tcaaaggcat gccgatcaag
      481 atcaagaatg aagggatcgc aatctctaag tttccccatt cgatttgtcg caaagattgt
      541 gccatcaata ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt aacaactgat
      601 agtaatggag aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat agctgtgata
      661 cctgaacgtc ctccaacggc gataccagct gatgattcta caaatgcacc aacaggagaa
      721 gtaccaaata aggaacccgc aagcatagct gttgaatcag aagctaaagc tttccctaca
      781 cgaggcatct tgttgtcttt catgaaacct gcttggtttg ctaatccaac taaagttcct
      841 gctgtatcaa agaaagtcac aagcaagaat gtcagcacta cgacccacat ttgaagagaa
      901 ttgatgtccc ctacatgttt taatgcaact aaaaatgttg gtttcaaact tggtgctgcg
      961 gcaacaattt cagaaggcat tggaatcaaa cctgtgacca gacctaaaat cgttgtagct
     1021 gccatcccga tgaagatgcc acctggtaca cgccgaacaa gcaagatagc agttacgact
     1081 aatccaaaaa tcgtcaacca agtagaacca acgttgagtg atcctaatcc tactaatgta
     1141 gattcattcg aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat aaatagaccg
     1201 ataccgccag aaatcgcata tttcaaatct gctgggatcg catcaatgat catttcccgc
     1261 agcttgaaga ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc ggctaatgct
     1321 gtttcccatg gaatccccat tcctaaacaa acagaataag caaaaaaagc attgatccca
     1381 agagcaggtg ccgttccaat tggataacgg gctaataccc ccattaaaac acagccgatc
     1441 gcactggcta atgcagtcgc agtaaacact gctccttcat ccattccaga agcacctaat
     1501 acagttggat ttacaaataa gatataagcc attgagataa atgttgtaaa tcctgccagt
     1561 acctcacgtt ttaccgtggt gttcaatttt tctatttcga agtaggaaag tattttttcc
     1621 ttcat
//
LOCUS       DASJON010000313         1620 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.320, whole genome shotgun sequence.
ACCESSION   DASJON010000313 DASJON010000000
VERSION     DASJON010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.320"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            96..1415
                     /locus_tag="U3989_002874"
     CDS             96..1415
                     /locus_tag="U3989_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEN1716280.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            1387..1584
                     /locus_tag="U3989_002875"
     CDS             1387..1584
                     /locus_tag="U3989_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716281.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
BASE COUNT          585 a          246 c          364 g          425 t
ORIGIN      
        1 cagtaaagaa agtataaaaa gttaagggct aaaaaggctg cactaccagt cttttagccc
       61 ttttcatttt gtttttgaac aagtaaaata ttactatgaa agcgaatatt ctcgaagcaa
      121 tcttttttga cgaacaccat aattgggata actttgttga gaaatacggg cgaaagattc
      181 ggctagtcgt caaacgggaa gtaaacaagt ttagatattg tggagatatt caaaaaggat
      241 accgactgtt tgtctgcgag ggatgtcatc aaacaaagtt tgttcccttg aaatgtcgtg
      301 gaaagttctg tccaacgtgt gcaacaggtg aaagccaaag gtggtcagcg gttgtggcga
      361 atgatatgtt taaagtgaca catcggcacg taatctttac gatcgatgag gggttacggg
      421 aaatctttat gatggaaaac tatcgagatt ccttactgaa aggactaatg gatgaagccg
      481 caaaaattat tcttgcctat tttgagaaaa gaaaggtgaa ggcaggtata gtagctacaa
      541 tccacacctt tggatcaaaa ctagaattta atccgcatgt tcatatggtt gttactatgg
      601 gtggcttaac aagagatgga agttgggaag aatacgatta tttacctttt tctatgttaa
      661 gaaaatactg gaaaaatgct gtattgaaac taattagacg aacgttaggt gagtgggaca
      721 agcaaagagt tcaatcaaga ttacaagctg cgtacaagaa caatggagaa gggttttacg
      781 taaatgctcc taaaaggagc aggacaaatc taaagtcact actccaatac atcagtcgtt
      841 atatgaagcg tggcccgatc gcattaagcc gaatcttaat gtatgatggc gacactgtca
      901 tgttcaacta catggacaaa cgaacgaata ctaaagagac gatgaccatg agtatagacg
      961 aattcatagc tgcactgatt cgccatattc aaaataagaa tttcaaaacg attcgtcgct
     1021 atggaatcta tagtcgtaga atcaaaacat taatgaaaaa ggtcatgaag gagtatcaga
     1081 aacgagtaaa aaagctgttt atcaatctga atgaggcctt aagaccaaaa tcctgggaag
     1141 aaagaataac cgaagaattt ggtgaaaatc ccttagagtg ttcttgttgc ggtgagtact
     1201 atgaattcgt tgggatgagt gtgcgcaaaa atggacggtt agttattaaa tatgcgaaag
     1261 ataaccatgc aaagcggttt atgcgagagg agaatcgaaa aattgaccaa aaagaattca
     1321 ggcaacagta tgaagaagaa aaagaaaaaa cttttaaagc aattcgctgg gattgggaac
     1381 gccaacgtga aatttatatg tctaagttgt ggtgaaaaag aatcaatccc aatggatgta
     1441 gtaaaatttt tagatgatgc agatattgca aatgatccga acaatcctcc ccaatttacc
     1501 tgtgaaaaat gtgggaagga gatgtatcca gaatattata gaaacgccct tggaatagag
     1561 tttaagatca gtgatgttca ataagaaggt taatcataca aaaagtccac cggagaattc
//
LOCUS       DASJON010000314         1598 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.321, whole genome shotgun sequence.
ACCESSION   DASJON010000314 DASJON010000000
VERSION     DASJON010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.321"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1598
                     /gene="brxC"
                     /locus_tag="U3989_002876"
     CDS             <1..>1598
                     /gene="brxC"
                     /locus_tag="U3989_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN1716282.1"
                     /translation="QTELKNKLLFVDSPELKLFQDAENYAEVYPFIPYQFNLLGQILT
                     EIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQFYYSLERWIDTAISR
                     VITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVENITSLMIRNINEDRL
                     ETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQNITDSDLIVELSKRI
                     FDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAGNAEMTVNFISPYSD
                     LAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTTYSSVVKNFDAIRSE
                     KSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQSALQKMAEEVYHKL
                     NQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQKRNHLTITMKTIRDR
                     FETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNSIVMIIQKRRESEKI
                     KIEPSAVISPKAKKLVDEIGRELF"
BASE COUNT          611 a          246 c          283 g          458 t
ORIGIN      
        1 caaacagaat tgaaaaataa attactcttt gttgatagtc ctgaattaaa gctcttccaa
       61 gatgctgaaa attacgcaga agtttatcca tttattccct atcaattcaa tttattgggg
      121 caaattctaa ccgagattcg taatcacagc tatcaaggat caaacttatc gtctggtgaa
      181 cgttcattac tagcaatgtt taaagaagcc gctgaacgaa ataaggaaaa tgatacaacc
      241 agtttggttt ctttagatca gttttattac tcgttagaac gttggattga tacagcaatt
      301 agccgagtga ttacacaagc tacaaataat tctcatattc aaagaccaac tgatatagat
      361 aactttaacg taaatgtctt acgaatttta ttcatgatta aatatgttga tcaagccatc
      421 aaaccaaatg ttgaaaatat tacgagcttg atgataagaa atatcaacga agaccgcctg
      481 gaaactaaaa aacgagtaga agaagcattg gaccttttaa ttaaggaaaa ctttgtgcgg
      541 aagaacttag atagttatat tttccaaact gatgaagaac aggaaattac tcgtgagatt
      601 aaaaatcaaa atattacaga tagtgatttg atcgtagaac tatctaaacg aatttttgac
      661 ggcgttctag accgaaatag ttttgcctat gaaaaattag gcatgtcagg tggaaataaa
      721 tttaaaaacc ggtatacgtt tacttatgca caagtgttag atcaacgtcc atacaagcaa
      781 gctggaaatg ctgaaatgac agttaatttt atttcaccat actctgattt agcaggtcaa
      841 gaggcagaac ttgcgcttaa gagttcccgt aacttattat tcgtagaatt gccatcagat
      901 aaagaattta aagatgatat agcaatgtat gctagaatga atctttattt aacaacgact
      961 tattcgagtg tagtaaagaa ctttgacgcg attcgttcgg aaaaatctgg tgaattacag
     1021 gaactaagtg caagtatcaa cgatcaatta aatgaactgt tgaaatctgc aaaatttttc
     1081 tataacggtg cagaaattca actgacaggc tccgacttta aagcaaaatt ccaatcagca
     1141 cttcagaaaa tggctgaaga agtataccat aaattaaacc aaattgatgc gcctaaagaa
     1201 aaagaagata tcttatattt agtgaaagaa agcaaaaatg aaattattga agatccatca
     1261 aatgagcaag cagttcagga aattttcaat tttatcaatg atcaaaagag aaatcaccta
     1321 acgattacga tgaaaacaat tcgtgatcgt tttgaaacaa aacaaccgta tggattcaca
     1381 gaacttgatg tggaatggtg catcgctaaa cttttcatgt ctggtaaaat tgatttactt
     1441 tataacaatg tacaaatttc acggatggat gatccaaata gtattgtaat gattattcaa
     1501 aaacgacgag aaagtgaaaa gataaaaatt gaaccaagcg cggtgatttc gccaaaagca
     1561 aaaaaattgg ttgacgaaat cggtagagaa ctgtttca
//
LOCUS       DASJON010000315         1597 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.322, whole genome shotgun sequence.
ACCESSION   DASJON010000315 DASJON010000000
VERSION     DASJON010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.322"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..164
                     /locus_tag="U3989_002877"
     CDS             <1..164
                     /locus_tag="U3989_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1716283.1"
                     /translation="AKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDCYQECMLAIYL
                     AISKFEIKE"
     gene            678..1010
                     /locus_tag="U3989_002878"
     CDS             678..1010
                     /locus_tag="U3989_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1716284.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            920..1123
                     /locus_tag="U3989_002879"
     CDS             920..1123
                     /locus_tag="U3989_002879"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1716285.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1307..1462
                     /locus_tag="U3989_002880"
     CDS             1307..1462
                     /locus_tag="U3989_002880"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1716286.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          577 a          273 c          282 g          465 t
ORIGIN      
        1 tagcaaagaa tggaaatgaa gaagcagttg tggccattct caaaagattt agcgctttgg
       61 tccataagca atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc
      121 ttgcaatata tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc
      181 tgtgacataa gtaaaaagat agtttcaaat ttcgaacaat aaatttgaag ccatcttctt
      241 tataattata aagtatcgat tttacagcgt tctataatga ttcatagaat ctaaaacgaa
      301 tgattttaat tattaacgtt ttttaattcg tttttagctt aaaaataact tttgtcacag
      361 tctcactcct aaaattactt tatttacgta cgaagtgggc aggaggtcga atccagaact
      421 gggccatggt acttcaacga ggacttctcc tctagaattg aaaagcatgc gatttacctt
      481 ccgtaacgac ggtagattta ttcaactatg tgtccaatca aaatttgtct gatttcaaaa
      541 atagtcaaga gaattttggt tgttaaaaac tcttatctat caacttccac agtttagttg
      601 acaaaccctg atactaagtt gggctatgat acacttttct tgactatcag ttacgttacc
      661 ggaaggaatg aactctattg aaaaaaacat atgatcgtaa atttaaacta aagatttcac
      721 aggatatcct tgagaaaaaa gtaactacca agaaaattgc tgaagaatac aatatctctc
      781 gtccaacaat ttcccgatgg gtttcagaat atcgtcgata cgggaaaaat gcttttgctg
      841 gacaaggaaa aagattacct gataaagcag atttctacat tcttgaacag gaaaataaac
      901 gactaaaaga ggaagacgag tgttctacga acacaaagat cgatacggat ctccagggat
      961 cactcaaaaa ctttacgatg aaggaatcga aatcaataaa cgtgttgtag ctgttcttat
     1021 gcgtgaaatg aatttatgca caagaggatt tcatcgacgc aaatcttctc atggtagaga
     1081 aaaaacaatc aaagaaatgg taaaaaaaat gttgaatcga taatttgaac agaaccaaat
     1141 tgatgctgtt tgggtgacgg atattactta cattccatgt aatgacggtc gcctctattt
     1201 gtccacctat attgacttag caactcgtat tctacgttac tatatggtgg attcacacat
     1261 gaaaaaagag attgtcgtcc gtccattaca aatttattaa ggagaattgc ctacggtaat
     1321 tcattccgat agaggtagcc agcatcggtc ttttatttat caagagctgt tgaccgacca
     1381 ccacatcaca cattcaatga gccaaactgg gactcctgtt gacaatgcag taattgaagc
     1441 gtttcatcga tcaatcaaat gagaattaat cgactccaac aaatataaaa gtaaaataga
     1501 gatgaaggtg ttaatccaag attatttaga agactactac ccaaacaaaa gaatccatac
     1561 aaaatttatg atgactcctt gacaatttga agcgaag
//
LOCUS       DASJON010000316         1550 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.323, whole genome shotgun sequence.
ACCESSION   DASJON010000316 DASJON010000000
VERSION     DASJON010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.323"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(606..962)
                     /locus_tag="U3989_002881"
     CDS             complement(606..962)
                     /locus_tag="U3989_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN1716287.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /gene="spx"
                     /locus_tag="U3989_002882"
     CDS             complement(976..1386)
                     /gene="spx"
                     /locus_tag="U3989_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1716288.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          475 a          279 c          218 g          578 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt
//
LOCUS       DASJON010000317         1542 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.324, whole genome shotgun sequence.
ACCESSION   DASJON010000317 DASJON010000000
VERSION     DASJON010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.324"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..843
                     /locus_tag="U3989_002883"
     CDS             <1..843
                     /locus_tag="U3989_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1716289.1"
                     /translation="KESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQN
                     IVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKV
                     NKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKL
                     KAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLA
                     DTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIY
                     SRDH"
     gene            876..>1542
                     /locus_tag="U3989_002884"
     CDS             876..>1542
                     /locus_tag="U3989_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1716290.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPD"
BASE COUNT          601 a          176 c          271 g          494 t
ORIGIN      
        1 aaagaatctt tttctatagt tcacaatcag gtttattttg gcgggggaat taatttatgg
       61 ttagccaaaa agaatggaat cagacagaga attgctcata gccatgcaac tgaagatgga
      121 aaatctcaaa atattgtaat gaatgtattg cgtaaatttt taaccaaatt gttgttacaa
      181 aatgcaacag actaccttgc cgttagtcaa gaagctggtg aaagtctgtt tcaaaaccac
      241 ccctttgaga tagttcataa tggtattgat ttagagcttt atagtaaaaa ttcagaagct
      301 aaggttaata agagaaaaga attggatatc tcaatgagta cttttgttgt gggaaacatt
      361 ggacgattag agaaacaaaa aaatcaatct tatttgttag agattttttt agaaatacta
      421 aaaatgaatt ctgattctta cttgatttta gtaggtgaag ggtcacttag aaataaactg
      481 aaagccaaag cagtagaatt ggatatagag aaaagagtaa aatttttagg agaaagaaac
      541 gatattcccg agttattatc tgtgatggat gtttttgtaa tgccttctct ttatgaaggt
      601 ctgccaatgg ttggattaga agcacaagca gcaaaattaa aattagtctt agcagatact
      661 atttccaaag atacaaagtt aacaaataat gtggattttg tatcattaga tgaaacacca
      721 caagaatggg caaaaaaagt aataaaagag ccgtatgcta ataaaataac acaagaacta
      781 aagagttatg atgtttcgta cactactgaa caaatggaga gtatatatag tagagatcat
      841 taaaaatgtt caagatattt taggagtgat tatttttgaa aatatgttat ttgatattgg
      901 catacaatga tattggcaac ttaaagagat tggtcaatcg attgaacgaa aatgcagttt
      961 ttattattca tatagataaa aaaactgata tagaaccatt tattgaagca tttaaatcat
     1021 atgataatgt tgaattttta tgtaataaaa accgtgttaa aatttattgg ggtggttttt
     1081 ccattgtaca agcagaaatc aatttagtaa agagagcatt acaaaatgag aaatatttaa
     1141 aatatgtatt attatctggt gctgattatc caatcaaaga taatgagtat atttataatt
     1201 attttaaaaa gaatagttca gtggagttta ttcgtggaat agatctagat caaattaaac
     1261 ataaagaatt ttattataaa catatagatg tatatcaaaa acatgattat cctagaatta
     1321 ataggaataa cacaactgca tttaaaattt ttagagctat aattaatcgt tgtctgagga
     1381 tgattaaact tcccccaaaa attagacacc ataaatttga tttgtatcat ggttctcaat
     1441 ggtgggcttt gtcgaaagaa tgcctaacag aattaataca aatgtatgaa caaaatcaac
     1501 aagactacct gaattttaaa ataggtatgt ttgccccaga tg
//
LOCUS       DASJON010000318         1536 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.325, whole genome shotgun sequence.
ACCESSION   DASJON010000318 DASJON010000000
VERSION     DASJON010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.325"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="U3989_002885"
     rRNA            complement(<1..>1536)
                     /locus_tag="U3989_002885"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DASJON010000319         1525 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.326, whole genome shotgun sequence.
ACCESSION   DASJON010000319 DASJON010000000
VERSION     DASJON010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.326"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..402
                     /locus_tag="U3989_002886"
     CDS             <1..402
                     /locus_tag="U3989_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN1716291.1"
                     /translation="DALVIKKEQGFTGTETKSLFLKDKKDNNYVYLTFTTKQTDFKAL
                     GKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGRPD
                     QTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            439..1449
                     /locus_tag="U3989_002887"
     CDS             439..1449
                     /locus_tag="U3989_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN1716292.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          541 a          242 c          335 g          407 t
ORIGIN      
        1 gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc
       61 ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat
      121 ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg
      181 gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt
      241 ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga
      301 ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag
      361 atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat
      421 acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg
      481 gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta
      541 aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat
      601 aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat
      661 gaaaatatcc aaatgatctt tccgacttgg ggaggagaat tattgattga gattctcgat
      721 tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg
      781 ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac taaccttatt
      841 gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca
      901 agagaaggag aatcgattct acagcaatct tctgaaaagt atcaaaaagt gtggccccat
      961 gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc
     1021 ggagatgcag taaggatgaa gggacgatta ttaggtggtt gtattgatac gatcaggcat
     1081 ttaatcggta ctccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag
     1141 ccgatcttgt ggtttctaga aaactgtgag ctatcgtcag ttgatttaag aagatcactt
     1201 gttcagatga aactggcagg ctggtttgat ggcagctcgg ggatcatgtt cggtagaagt
     1261 gcggcagagg atttgttaga ttataaaatg aaggatgtat acgaagatct gtcaaaggaa
     1321 ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg
     1381 attaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg
     1441 tttatttaaa aaattttttc ctgccttgga gtctatcaaa gaccaataaa tctaaataga
     1501 aaagaaagtg aaagaagctg acaaa
//
LOCUS       DASJON010000320         1513 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.327, whole genome shotgun sequence.
ACCESSION   DASJON010000320 DASJON010000000
VERSION     DASJON010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.327"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            65..403
                     /locus_tag="U3989_002888"
     CDS             65..403
                     /locus_tag="U3989_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716293.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(608..847)
                     /locus_tag="U3989_002889"
     CDS             complement(608..847)
                     /locus_tag="U3989_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1716294.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(823..1170)
                     /locus_tag="U3989_002890"
     CDS             complement(823..1170)
                     /locus_tag="U3989_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1716295.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT          462 a          304 c          261 g          486 t
ORIGIN      
        1 taccttcaat tttgcagata aaacgctttg ggagaaaatt tcaaagacaa caggccatcc
       61 tatcatgccc gattataaac aaaaagctgg ctattccttt tgctctgaca aatttcaatt
      121 gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatca aagaatgccc
      181 gcttcaaata gaaacattgg ttcaacgaat ttcaactaga caagactttg ctattgtaga
      241 acgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa ctcatatcaa
      301 tgtggataaa tggcaacctc ttatctataa attcagagaa tatacaactg ccaaccagtc
      361 gttaactttg aattttagat tccaagaatt caaagagtca taatgataaa catcgttagg
      421 tataaataaa aaagactcgc agcacgagta aaaaacaaat agaagctata cattagaaag
      481 gtcgactgtg accctataga tgcagtatat gcagcttatg agcatttctt ctgttctctt
      541 tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct ttgaaaaatc
      601 gattatgtta taccttctcc attactaatt caggcatctg ctgagtaatg atttttcgcg
      661 caaaagaaat atctgttacc caaggttcga tttgttcttc agtcaagaca agcggcattc
      721 ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt gattctgcca
      781 ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt tccttatttg
      841 aaaacataaa tttgtctttt tcttcattcc actcattggg aaaacacaac gattttgcaa
      901 aaatggttga tgaaaggtct ttttttcttc tactgtctct gcacgggcat taatcatcaa
      961 ttgtcctttt ttaaatccgg taaatcccca cttcatgatt tccggagtga ggatgttttc
     1021 ttcctctttt cttaacgtca caacaagatt tgacggaaaa acttctccag ctgctaattc
     1081 tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa taattctgaa ggagctctga
     1141 ctctagatca aaaaaataac gaccacacat cctaatccct cccaatcttt ttttctatca
     1201 tacactgcaa caagaagttc gcaccaacaa aacgctttca atttttgtag aactaaaaaa
     1261 gttgtgacaa gtgacaaact ctccagtgaa tggtattctc ctgccaactc ctcggtcaca
     1321 agtcacaata ttcaacaaac atgagaagct gctttttgaa attgttcctt gtgactcgga
     1381 gtgaaacggc aggcttacga cctctgggta aacggtgttc aaaagccagt ttctcggtat
     1441 taagccaaat ttatgcaaaa atgtagaaag ctattttcac aaaatttttc ttaatactcg
     1501 aaactaagcg act
//
LOCUS       DASJON010000321         1491 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.328, whole genome shotgun sequence.
ACCESSION   DASJON010000321 DASJON010000000
VERSION     DASJON010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.328"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..>1491
                     /locus_tag="U3989_002891"
     CDS             87..>1491
                     /locus_tag="U3989_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1716296.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENG"
BASE COUNT          435 a          292 c          297 g          467 t
ORIGIN      
        1 tctcttttca ttctaaattt cctaagctac aatgtggtag tacttgtttt ttcaattgtg
       61 gacaaataaa taataggagg aatcatatga ttcagttatt acgttatgca aaagactatc
      121 gtaagcaaat cattttgggc ccgttcttca aatttcttga agctgtcttc gaacttgtcc
      181 tgcctttaat gatggcttcc ttgatcgata atggactaaa aatgaatgac cgaggaaaaa
      241 tcattgaaat gggcttatgg atggtcgcta tgtctgtgat tggtctgatt tgtgctatca
      301 tttgccaata ctatgcttca gtagcttcac aaggatttgg tacagagtta cgtaatcagc
      361 taatcaaaaa aatcaatact ttctctcaca acgagttgaa tcattttgga acagatacct
      421 tgatcacccg aatgacaaac gacatcaatc agcttcagct agctttagca atggtcattc
      481 gtttattgat cagagcaccg tttttaagta tcgggtcagt ggtcatggct tttgtgatcg
      541 actgggaagt aggtctgttt ttcttagcat tattgcctat tttctcaatc attttatttt
      601 ttatcatcaa aaaaaccgtt cctctctacc aaaaagtgca agaaaaacta gatcagctca
      661 atgaacacgt tagtcagaat cttagcggtg ttcgtgtcat ccgcgcattt gcgcagacaa
      721 aaaaagaaac agagaagttc gataaaagca caaatgatct agcgaacaat tatttacgtg
      781 tttccaatat ctctgcccta ttatcacctg ctacaacctt gatcatgaat gtcgggatca
      841 tctgtttgct gacagttggc gggatcaaag taaatattgg tagcttgcaa caaggacaag
      901 ttcttgcttt gatcaattat atgaatcaaa tgcttttggc actgatcgtt gtttcaaacc
      961 tcgtcgtgat cttcacacgt gccgaagctt caggaaatcg ggtgaaagag gtgctagaca
     1021 cagaaaattc gattcttgaa gctgaggtca gtactactcc cgatttttct tctgaagcaa
     1081 ttatcgcttt tgaccatgtt gacttccgtt atactcctga ttctggtctt tctctccaaa
     1141 atattacatt tcagctgaag cgaaatacag tccttggaat cattggtcca accggaagcg
     1201 ggaaaacgac tttgacacaa ttgattccgc gtttctatga tgtcagttcc ggtacagtta
     1261 taatggacgg aatggatgtt cgttcttggc agctggatca tctccgtcag caaattgctc
     1321 ttgttcccca aacttctgta ctatttaccg ggactattcg tgaaaacctt caatggggta
     1381 agaaagacgc cacagaaaaa gaatgttggg aagctctgaa aatcgctcag gcagaagaat
     1441 ttgtccgtca gcttccaaat ggtttagata caaaagtcat ggaaaatggg a
//
LOCUS       DASJON010000322         1440 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.329, whole genome shotgun sequence.
ACCESSION   DASJON010000322 DASJON010000000
VERSION     DASJON010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.329"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U3989_002892"
     CDS             complement(99..1229)
                     /locus_tag="U3989_002892"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN1716297.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          409 a          285 c          233 g          513 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
//
LOCUS       DASJON010000323         1433 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.330, whole genome shotgun sequence.
ACCESSION   DASJON010000323 DASJON010000000
VERSION     DASJON010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.330"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..298
                     /locus_tag="U3989_002893"
     CDS             <1..298
                     /locus_tag="U3989_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEN1716298.1"
                     /translation="DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLC
                     KDLKAYIINTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            474..1217
                     /locus_tag="U3989_002894"
     CDS             474..1217
                     /locus_tag="U3989_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1716299.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1210..>1433
                     /locus_tag="U3989_002895"
     CDS             1210..>1433
                     /locus_tag="U3989_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN1716300.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVA"
BASE COUNT          531 a          204 c          279 g          419 t
ORIGIN      
        1 cgatgaaatc ctttatctaa tgagcatgcc aggttataaa aataaaatag gcctagtatt
       61 gaaagatgaa gagattctcg taaacggcac aattaaaaaa tataaaaaat cactcgaata
      121 tgagtattta tgtaaagatt taaaagctta tatcattaat actgaaaaca ttacatctat
      181 tagcagagta aacgaaatag taacatttgc aaatggagaa caactagaga tgggaaagag
      241 aagtattgat aaactaaaga attttatgca agaaaagata gaaaatgaat cattttaaag
      301 aaaacaaaga ctcatatctt acctgttaga agagtgtttt cctgtagaag agaacaaccc
      361 aagtagcagc ctagtcgttt ctatcctttt ttgttatgca ctctttttga catttactcc
      421 accaataacg aagggtacaa taaaaagtaa taacaaacaa aaggagtaca gaaatgattg
      481 aattcatcaa tgtaagtaaa agttacggga aaaaacaagc attaaaagac ttgtctctct
      541 cgataagaca aggggaaatt tttggatttt taggacataa tggtgccgga aaatcaacta
      601 cgattaaaag tttagttagt attattgaac catcatcggg aacgatttta gtagatggaa
      661 tgaaattgac ggaaaatcga ttgagtatca aacaaaaaat cggttatgtg cctgattccc
      721 cagatatttt tttacaattg acggctggag aatattggga cttgatttca gctgcctatg
      781 aattagacac acaaaaaaaa gaaaagcgtt tagcagaatt gacagcgctt tttgatatgt
      841 atagtcatca aaatgaaaca atcgcaagtt tttcacatgg aatgcgtcaa aaaacgattt
      901 tgatcggcac gttgctgcca gatccagata tttgggtatt ggatgaacct ttgcaaggac
      961 ttgatcctca agcagcattt gatttgaaag aaatgatgaa ggctcatgcc gcaaaaggaa
     1021 aaacagtcat tttttctaca catgtattgg ataccgcaca acagttatgc gataaactgg
     1081 ctattttgaa gaaaggtgaa ttgatttatc agggggcagt tagtgaatta ttgaatggct
     1141 cacctgatga aacgctagaa aagatttatt tgaaaatggc tggaaggcaa gcgacaggag
     1201 atggaccgta tgtataagcg acaattatta gaattaattc gagttaatct tttatatgtt
     1261 aatccacagc tcacgaataa agcaagagag aaaggaaaaa agggaaagcg attaactatc
     1321 tatcttttgt ctcaaaatct tttttcaggg ctgatttttt tactgattta cggatttgct
     1381 atgtttgcta tgaactttgt taaattgcca ggtttcttta cttattacgt ggc
//
LOCUS       DASJON010000324         1428 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.331, whole genome shotgun sequence.
ACCESSION   DASJON010000324 DASJON010000000
VERSION     DASJON010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.331"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            178..774
                     /locus_tag="U3989_002896"
     CDS             178..774
                     /locus_tag="U3989_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1716301.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..>1428
                     /locus_tag="U3989_002897"
     CDS             898..>1428
                     /locus_tag="U3989_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEN1716302.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLV"
BASE COUNT          390 a          229 c          254 g          555 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgtt
//
LOCUS       DASJON010000325         1420 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.332, whole genome shotgun sequence.
ACCESSION   DASJON010000325 DASJON010000000
VERSION     DASJON010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.332"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U3989_002898"
     CDS             complement(150..1337)
                     /locus_tag="U3989_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN1716303.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          309 c          249 g          449 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact
//
LOCUS       DASJON010000326         1362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.334, whole genome shotgun sequence.
ACCESSION   DASJON010000326 DASJON010000000
VERSION     DASJON010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.334"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..624
                     /locus_tag="U3989_002899"
     CDS             <1..624
                     /locus_tag="U3989_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN1716304.1"
                     /translation="DIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSI
                     TLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISS
                     VVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLK
                     SNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            769..1074
                     /locus_tag="U3989_002900"
     CDS             769..1074
                     /locus_tag="U3989_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716305.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          503 a          153 c          244 g          462 t
ORIGIN      
        1 gatattcgaa atttagaaaa catggttttt aatctgagta atggaataat tccaagtgtt
       61 agtggagata cgattcggtc tgaaaaaaat tattctatca ttgtttttga aaagttagca
      121 caaacgtcta taacattagg aatggatata atagaagcgt atcaaagtag agatgctttg
      181 atacaagaaa acgaattagc tgtatcgtta cctgaggtct taaaagtgag ggattctggg
      241 attgtttatt atactaagga aatcggaaaa actaagatag agcatctttc tcccttaatt
      301 tcttctgtcg ttcaattcat cggcttaaat atctataaaa gaattactgt aaaggaaata
      361 gctaattatt tttctgtaag tgaaactaaa ttaagagaaa gctttaagaa tgaaatgcat
      421 attacgattt ataattatat ctctaagcga aaaatttcta cggctaaaat aatgttgaaa
      481 tcaaaccata ctatcagtga agtttcttta ggtttaggat tttcagattc ttcacacttt
      541 tcgagagtat tcaaaaagta cgcaggtgta tctcctaagc aatatcaatt aggtttagtg
      601 gataatttca acaacattag ctgagaggtt ttattttatt atgagactat atcatttacg
      661 tagtttggag gagttgagga aattggattt taacaaaaaa ttttttggga aatttctgat
      721 ttgagttgtt tcgtagaatc tgagcgtcta agtaatggta ataatagtgt gttaaatgaa
      781 tatgacaaag tagtaattaa taaagtaaaa agtttatgtg aagaagctag agaatctatc
      841 agtcatatta atggtagcaa ttttaagcaa acattattta atctgattaa gctagatgct
      901 aaaatatcaa gttatttatt ctttttaata catgatgaat ttatggatta tcaaaaattg
      961 gatcagttaa ttgaaagaga aagctgggaa gattattacg tgggtctaac tttttcagaa
     1021 aagttaaaca actacaagtt aatagattat aagtatttga gtgaaagcaa ttaattattc
     1081 atgtagagta ctagacactt taaatatgga caattcaatt ataaagaaaa agaatcaata
     1141 atttaatgag gctgtgacaa atgtcatttt ttgtttcgct aaaaacgagt gaaaaaatgt
     1201 tgataactcc aatcattcgt ttaagattct aggaatcatt caggaatgct gtgaaaccga
     1261 tactttataa ttataaagaa ggtgatttca aattccgaag aaggaatttg aagtcacctt
     1321 tttacttatg tcacagtctc attcagtaat gcgggataag gc
//
LOCUS       DASJON010000327         1351 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.335, whole genome shotgun sequence.
ACCESSION   DASJON010000327 DASJON010000000
VERSION     DASJON010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.335"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1301
                     /locus_tag="U3989_002901"
     CDS             <1..1301
                     /locus_tag="U3989_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN1716306.1"
                     /translation="VKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDI
                     GRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSL
                     VAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYL
                     NQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKE
                     FTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWF
                     VLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGF
                     EPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLN
                     PVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          450 a          244 c          288 g          369 t
ORIGIN      
        1 cagtaaaagg acatcaacga gttgtaggaa cgaaagcaga caaaatcaaa cacttaccta
       61 taacagagaa agaacatctc cttcctttgg aagaacaatt ttgtgaacat tgcggttcac
      121 agatgaaaga tatcggacgg acaaaagtac gagaagaaat tcgtttccat caggctatgt
      181 tggattgtct cacccattat caacatacct attgttgtaa aagctgcgaa aaagaaggac
      241 tttcttcttt taaaaaagcg atcgttccta aacctttgat ttctaatagc ttaggttcca
      301 atagtttagt ggcggaaacg atccggatga aatttggtca gaaagttcct gcctatcggc
      361 aagagaacta ttggaaacaa acacatgggt tggatatttc gagagataat atcaccaatt
      421 ggcatattaa agcagtgcaa aacgcgttag accctttagg agaacgatta agagtctatc
      481 ttaatcaaga agagattcta cacggggatg aaacaagtta tcgtgtgatt gaaagtgcca
      541 aaacagatac ctattactgg cagttttgta caggaaaaga cagtcagcat cccatcgttt
      601 attatcacca cgacgaaagt cgtgctggag atgttccgaa aacttttctc aaggagttta
      661 cagggtattt acactgtgac ggctatagcg ggtataatgc cgtggaaagt gtacgacttg
      721 tctattgttt cgcgcatgtt cggcggaaat ttttcgaagc aattccaaaa ggaaagaaaa
      781 acacggatat tcccgcagct caagcagtca aacaattgga taaatggttt gtcctagaaa
      841 agaaatggaa agacttttct cctgagaagc gattaagctg tcggcaacaa gaactgcgtc
      901 cattatttat tgctttttat gagtggatgg caacgataga tcctgttgca aaatccaagt
      961 tggatgcagc cgttcaatat gcgtgtaaac tacgaagtgg ttttgaacct atttttgaag
     1021 atggtcgttt agaactcaca aataatcgtg cggagcgaaa tatcaaagag ctggtgatag
     1081 gacggaaaaa ttggttgcat tccacaagtc ttgaaggagc acgaacttca ggaatcattc
     1141 tgagtgtata taaaacagca gaacttaacg gattgaatcc tgtaaagtat ctagagtttc
     1201 tatttgataa gataccaaat cttcccgtac tttcagcaga aactttagat caactgttgc
     1261 cttggaacaa ggatgtacaa caacattttt ctcgtaacta aaaacgccaa acattcccga
     1321 ttctattcta taggtcgggt tatttgacgt a
//
LOCUS       DASJON010000328         1319 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.336, whole genome shotgun sequence.
ACCESSION   DASJON010000328 DASJON010000000
VERSION     DASJON010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.336"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1285
                     /locus_tag="U3989_002902"
     CDS             <1..1285
                     /locus_tag="U3989_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1716307.1"
                     /translation="IKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEIRRCPLC
                     KQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTYIVDRNC
                     FIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWLPRCLLM
                     DEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKVKVIVTD
                     MNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQAKYYRQL
                     KALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFYYQEILQ
                     AFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKIENLHTH
                     IKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          429 a          261 c          245 g          384 t
ORIGIN      
        1 tatcaaacta ctacttggaa tcaaagataa aaacattttc atcacaaatg tggaatcgaa
       61 gagcattaag aatatcaaga gtcttgttgt ttctgcgacg ttaagtaaag aaattcgtcg
      121 ctgtcctttg tgtaaacaga tgaatcatga aggcatgatc gttaagaacg gaaagaaaaa
      181 atcgctcatt caacttaata aatgtgcgaa ccaactgact tatcttgctt tagcgaaaca
      241 acgctaccac tgtcgtggct gccacaccta ttttacggcg aatacctaca ttgtcgatag
      301 aaactgcttt atcgctaaac aagtgcgcta taaaatttta gaagagctaa ctgaaaaaca
      361 agcaatgacg acaattgcta agcattgcgg cgtttcttgg tcgaccgttt ctcggacact
      421 cgcttctctg atccccatga caaaagtaaa aagaaattgg ttaccgcgtt gtttattaat
      481 ggatgaattt cgttcactga aaaatcaagt cggtccctac tccttttctt gtatggatgg
      541 cgatactgga aaattactgg atattcttcc ttcacggaag aaaaaggctc tcgtttctta
      601 ttttatgcaa tttgaacgac gcgcacgatt gaaggtaaag gtcatcgtga cggacatgaa
      661 tgtttcttat gcctctctga taaaggaatg ttttccaaag gcaaaactcg ttgtggatcg
      721 ttttcacatt gtcaaacatc tcattcgtaa atttgaggat attcgcgtac gaatcatgaa
      781 aagctttgat cgaaacgatc caacacaagc caaatattat cgacaattaa aggcgttgtc
      841 tcggcttctg atcaaacgac aggacaagct tgtttatgac aaatggacca aatggcgcaa
      901 ttttggttgg gcgtatctca ccgaaagtga agtggtggat cgtctgcttt cgatctctga
      961 cgaattacga atcgcctatt tttactatca agaaattctt caagcgtttc acgacaaaga
     1021 ggcggacact ttctttaaac ttgtaagaac gatgcccaat tccgttccaa aagaactgca
     1081 ttctataaaa aaagctttta taaactatga atccggcatt cgtttagccc tcgaattgcc
     1141 ttattcaaat gccaaaatag aaaatctgca tacccacatc aaagcattga aacgagtcgc
     1201 ttatggcttt cgaagctttc ggaaaatgaa gaccagaatt ttcttactaa ataatctgat
     1261 tacctatgaa tcaaaaaaca tctaaagtag aaaaggaatt ctaccttaga tgttcttat
//
LOCUS       DASJON010000329         1314 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.337, whole genome shotgun sequence.
ACCESSION   DASJON010000329 DASJON010000000
VERSION     DASJON010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.337"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..>1314
                     /locus_tag="U3989_002903"
     CDS             133..>1314
                     /locus_tag="U3989_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1716308.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     AL"
BASE COUNT          458 a          234 c          266 g          356 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tctt
//
LOCUS       DASJON010000330         1313 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.338, whole genome shotgun sequence.
ACCESSION   DASJON010000330 DASJON010000000
VERSION     DASJON010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.338"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(185..1096)
                     /locus_tag="U3989_002904"
     CDS             complement(185..1096)
                     /locus_tag="U3989_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN1716309.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          384 a          267 c          185 g          477 t
ORIGIN      
        1 gctagcaatc ctctagataa tatttttccc aatataattt cctctaattc ttttgtttaa
       61 gttgtatcat tagtatagca atgtcctagt taacgtgggt tttaaattaa aaagctaacg
      121 ttaccccaaa agtgctagta caataatgct gttcattaat ttcgttggtt tcattccgcc
      181 atccttacct ccttgaatta gtatgtgttg atccgggaaa cactgtcaca attacaccgt
      241 ctatattgtt tcctgatatc tcataacgaa gattagtcgg cacatgaatg gtggtatttt
      301 tagtaacagg ataataaaag atctcatatt tttccccatt tacactaaca gaaatggttt
      361 tttcttcttt taggtaatcc atatattcct cgagaacgaa attcttttct ttcataatcg
      421 cactatgtgg taaaccaaca tagcgaatat gccatggttc atattgaatt cctgttaact
      481 ctgttttgtc ctctggataa cgtaaaatga acccgtattt ccaagcattt tcttctatcc
      541 actttccttc aggggctcgt tccattttcg tcaagcttga tcctacatct agtgataaac
      601 ctgaattatg ctcactataa cctgctggta aggcatactc agcccccatt tcttggtaaa
      661 gcacactttg ctcatcaaag tctcgatagc cactattaat aataaaatga ctaacgccac
      721 cctttacagc atcattgacc atctctgaaa atttttgtgc tatttctttt gacatataaa
      781 tattactatc aagcaacccg tatccattta ttaattcgtc atgtttagat aaattcacga
      841 tatctgactt cacactttct tggcgaacag gatatttact attgattaat agcagatttc
      901 cttgataaac ctgttcttct gtaattgttt tctcttgggt attttcagaa gtcccactat
      961 tttctaaatg ttctttggga ttttgatcat aattttgaaa ttcgactttt tcctgagaaa
     1021 acagtgcttc attaacgtag tcataaccta agtatattaa gaataacaat aacaataaaa
     1081 aaaacaactt cttcattttc agtctcctcc ttaactatat agttggaagt atgaaatatt
     1141 tctagaatct tccccttaaa cggcacaatc tcgtttgaga ttgtgccgtt ttgccccgta
     1201 aattacagtc aattatttcg ccctactgga tttactatta tcaccaatgt agatgaattt
     1261 tcataagtga tacccatgct gaatttaacg aggctgaact gcctgttgac aat
//
LOCUS       DASJON010000331         1297 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.339, whole genome shotgun sequence.
ACCESSION   DASJON010000331 DASJON010000000
VERSION     DASJON010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.339"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(433..>1297)
                     /gene="dnaB"
                     /locus_tag="U3989_002905"
     CDS             complement(433..>1297)
                     /gene="dnaB"
                     /locus_tag="U3989_002905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN1716310.1"
                     /translation="TIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARP
                     AVGKTAFALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSE
                     EEWSNLIVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEG
                     NGKENRQQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIE
                     QDADIVAFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYN
                     KFSSLSPREEF"
BASE COUNT          331 a          291 c          257 g          418 t
ORIGIN      
        1 ttgggcgtat taccgcagat gcaagctttt gaacaccgtt taagaaagag gttgtgagcc
       61 tgccgtttaa ctctgagtca caaggagcaa tttcaaaaaa ctgcttctca tgtttgatga
      121 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttaaggaaa agaggtcgta
      181 agatcagcgt tttgacctga gtattagaga aacaaacgtc aaaatagttc ctcatatttt
      241 gacgtttgct gagctaatac caagggtcag ctgattgaac accgtttatt cggaaaaagg
      301 gactgtgaca agacttttgt cacaatccct ttggagatct gtttaaatta agtactctag
      361 tcgttcctgc ggttactcct tgcatcttct tacgagagct ctcgggattg ctcaaggctc
      421 acaacctctt ctttaaaatt cttctcttgg cgaaagcgac gaaaacttat tgtattcttt
      481 gataaacagt agttcaactg ttcctcgtgc accgcttcgg ttcttctcga tgatgacttc
      541 gatgacgttg tcattttttg gctcgtttcc atcatcgtca tctccgcctt ctctttcata
      601 ataatcatct cgatacaaga aggctacgat gtccgcatct tgttcaattg atccggattc
      661 acgaatatca cttagcactg gtcgtttgtc ttgtcgctgt tctactccac gagataactg
      721 tgaaagagca atgacaggta ctttcagctc tttcgccagt tttttcagtt ggcgagagat
      781 atctgatact tcttgttgac ggttctcttt accgttacct tcaatcaact gcaaataatc
      841 gatcaatatc aagcctagat tgcctttttc ttgcgctagt ttccgacatt tcgcacggat
      901 ttcagagatt ttgatccctg gtgtatcatc gataaaaata tttgctcgag aaagactccc
      961 catcgcaacg atcagattcg accattcttc ttccgaaagc tgacctgtac gtaaatggct
     1021 ggcctcaatc gaaccttctg cacaaagcat ccgatttaca agagattctg cccccatttc
     1081 caaactaaat atcgcaacag aacgatctgt ttttgttcct acattttgag caatattcaa
     1141 tgcaaacgct gttttaccta ccgctggacg tgcagcaaga atgattagtt cttcgggctg
     1201 gaaacctgct gtcattttat ccagaccttg atagccagtt ggtagacctg tgatttcttc
     1261 attgttttga gctagtcgat cgatattttc aatcgtg
//
LOCUS       DASJON010000332         1282 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.340, whole genome shotgun sequence.
ACCESSION   DASJON010000332 DASJON010000000
VERSION     DASJON010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.340"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="U3989_002906"
     CDS             complement(31..1203)
                     /locus_tag="U3989_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEN1716311.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          346 a          266 c          245 g          425 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttatcttt ta
//
LOCUS       DASJON010000333         1276 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.341, whole genome shotgun sequence.
ACCESSION   DASJON010000333 DASJON010000000
VERSION     DASJON010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.341"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..172)
                     /locus_tag="U3989_002907"
     CDS             complement(<1..172)
                     /locus_tag="U3989_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716312.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
     gene            complement(173..1030)
                     /locus_tag="U3989_002908"
     CDS             complement(173..1030)
                     /locus_tag="U3989_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1716313.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          364 a          255 c          186 g          471 t
ORIGIN      
        1 tctgtctttc aacattggag gaagttttta tttcttgaac ttggtgcact tctaattgct
       61 tattttcaga tggtgtaatt gcttgctgct ttttctttgc ttctgcttct ttttttattt
      121 tggcgagtcc tttgttgttc ccaaaaatat caagtccttt gatgtcagcc aattactcca
      181 cctccgcata ccaattctcg tgttcttcca attctttcaa aatactgata aataattcgt
      241 ggcttttgcg atcccattgt tttaagagac ctttctttgt cttctccatc gtaatacctg
      301 atccgtcgta tcgttttaag cgcgcatttt tgagtacaac atttttaatc aaatgttccc
      361 cataaatctc ttgggcttgt ttatacattt cctgatctac tgcgtcaccg ctttccaaca
      421 tgaaaggaat aacaccagca acatataaat ttgtatcaaa ttctttcaca aaaaagtcca
      481 tatactcaag atattgcatc gcatttctca tcgattttac ttgggtctgt aagacgacga
      541 ttgcccaatc cgcaatgtac atcgcggatt tactatatac agaaatagaa ggtgggacat
      601 ctaaatagat gacgtcaaac tcatcaaata atggttctag cttttctttc atatatttga
      661 attgggataa tgaatcatta tattggtctt tgatccaatc atcatacatt tcaaaggaca
      721 tgtctgctgg aagtatatag aggttattga ttactttata gacagcctct ttaggtggaa
      781 gattgtttat caacacgtca gtcacagtat tttcgtaaac aatcggttca ccttctccgc
      841 ctccttcttc gttagtgaag ttcatatgcg ttaagttcat cacatcagaa gtatttcctt
      901 gcatatccaa gtcaattatc aaggttcttt ttccttcatt aactgctgat tcaaacccta
      961 acatttgcgc gcacgtactt ttacctacgc cacctttaaa attaccgatt acatatttct
     1021 tcgctaccac gttcctacct cttttctttt agcaatattc atttgatact attataacat
     1081 atttattttt agttactacc aaaaaaaaga tatatatgta ctaccttata attaataagt
     1141 aaaaggtagt acataaataa ggaaaagctg aaatatattg atttatagct ttcctcaaag
     1201 taatgacgtt atttaattta tcgagaagca gagaatactc gcgactttag tcatgtagac
     1261 gaatcagatt ttcgat
//
LOCUS       DASJON010000334         1274 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.342, whole genome shotgun sequence.
ACCESSION   DASJON010000334 DASJON010000000
VERSION     DASJON010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.342"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..1099
                     /locus_tag="U3989_002909"
     CDS             92..1099
                     /locus_tag="U3989_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1716314.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1111..>1274
                     /locus_tag="U3989_002910"
     CDS             1111..>1274
                     /locus_tag="U3989_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN1716315.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMA"
BASE COUNT          499 a          160 c          240 g          375 t
ORIGIN      
        1 gacatcatta ttgataatta gtttagttat atatttttta aagattaaga atcgaattat
       61 agatgactat aattcatagg aggagatata agtgtttaaa ggaaaaacaa tattagtaac
      121 tggcggaact ggttccattg gaagtgaaat tgttcgtcag cttttaaaat atgaaccaaa
      181 agctatcaga atttatagta gaggtgaaga aaaacaattt tatatgcaac aagagctctc
      241 aaatcacact aatttgagat ttttaattgg tgatatcaga gactctcaaa ggattgatta
      301 tgcatgtcgt gatgtggata ttatttttca tgctgccgca atgaaacacg ttccagcaag
      361 tgaatataat cctatggaag ctgtaaaaac aaatgtgatg ggaactcaaa atgtaataga
      421 tgcagcgatc aaaaataatg taaaacattt tgtaggtatc agtacagata aagtagtgag
      481 tcctactaac accatgggtg cgacaaagtt attgagtgaa aaattaataa tagctgccaa
      541 tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta aggtttggaa atgtcatggg
      601 gtcaagtgga tctgttattc ctctttttag taaacaaata aaaaacaata aagatattac
      661 tttgacagat gttgatatga cacgatttat gatgtcaata ccggatgctg caagattagt
      721 tctgtctgct gctatgaatt caaaatttgg agaaacatat gtcttaaaaa tgccggcaat
      781 caatatttta actttagctc aatcattaat aaaaatatat aatgatacac atgatgaaaa
      841 atatagaggt gaaataaaag ttgtaggtat caggccagga gaaaaattat atgaagagct
      901 aatgactaca gacgaaattg aacgctcata tgaaaatgat aaaatgtata tcattccacc
      961 acactatcat gctactgacg aactgtacaa agattatgaa aaaattaaaa tcactgcata
     1021 ctcttcagat gcaacagaat taatgagtgg acaggaattt attagaatcg caaaagagaa
     1081 taatgtcgtg acttggtaag gagttttatg atgaaatcag aagtatatgt aattgcagaa
     1141 attggtgtaa accataatgg aagtctagat ttagctttag aaagtattga taaagctaaa
     1201 gaatgtggag ctaacgcggt taaatttcaa acatttaaaa cgaataagct agtttccaaa
     1261 aaggctccaa tggc
//
LOCUS       DASJON010000335         1264 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.343, whole genome shotgun sequence.
ACCESSION   DASJON010000335 DASJON010000000
VERSION     DASJON010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.343"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..>1264
                     /locus_tag="U3989_002911"
     CDS             123..>1264
                     /locus_tag="U3989_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716316.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKG"
BASE COUNT          385 a          205 c          280 g          394 t
ORIGIN      
        1 atagaaaaaa gtgattttct aataaattat tcatgtggaa atgtcatttt tactgtataa
       61 ttcatttgat tatccatttt ttattcatca aaattactga aaaaagaaag gaagaaataa
      121 atttgaagaa aacaaattct tcaggaaaac cgaaacgcga cttcactaaa ttatcgacac
      181 cccatacgta tgtcattatt tttggtgtag tcatctttgc atggatactg acttttgtcg
      241 ttccagcagg aaagttcagc acgcaagata ttgaatacaa agatgcaaat ggcgaaacaa
      301 gtactcgtac tgttttacgt caagattcct tccgttatgc ttacgagctg gataagtcgt
      361 atgtgtttga tcaattagaa gaactgcaag atcatccagc agaacgtgaa aaactggatg
      421 ttccggaaaa aggattggaa aaagtcattg ctgacggaga aaaaaatctt acgcaggaaa
      481 aactagatga aatctccttg acagatgatg tgctttacga tcagtacggc gaaaatatct
      541 atgatacttc taaaaaattg cataaaacag ccaaaatatg gggaacagat gattttggcg
      601 gctttggttt cttaaatttt gtttttgaag gactagtatc tggagataag tatggatctg
      661 ctgtagggat cgctgcattg atccttgttg ttggaggtgc ttttggtatt attatgcgta
      721 ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa aactaaggga ctggagcgtt
      781 tagctttacc gctattattc tttgcttttt cattcggtgg cgctacattc gggatggcag
      841 aagaggtaat tccattctca atggtgatgg ttccatttgt cattgcgtta ggatatgact
      901 cgatcgtagc agtaaccgtg acttatgtcg catctcaagt cggaaatgcg acctcttgga
      961 tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg aatccccgtg ttgtctggtg
     1021 cgacattccg attgattatg tgggtagttg ttactgcatt agccgctggt tatatgatga
     1081 tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt gacttacaaa tcagatgatt
     1141 atttccgttc tcatatcaaa aagacatctg atgaaaataa aaaattcatg cttgggcata
     1201 aattgatctt attagagatg ttagttgtcc ttgtctggat cgtttggggc gtgactcaaa
     1261 aagg
//
LOCUS       DASJON010000336         1259 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.344, whole genome shotgun sequence.
ACCESSION   DASJON010000336 DASJON010000000
VERSION     DASJON010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.344"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..963
                     /locus_tag="U3989_002912"
     CDS             4..963
                     /locus_tag="U3989_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1716317.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="U3989_002913"
     tRNA            1152..1225
                     /locus_tag="U3989_002913"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          415 a          239 c          257 g          348 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaa
//
LOCUS       DASJON010000337         1255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.345, whole genome shotgun sequence.
ACCESSION   DASJON010000337 DASJON010000000
VERSION     DASJON010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.345"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            289..1104
                     /locus_tag="U3989_002914"
     CDS             289..1104
                     /locus_tag="U3989_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716318.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          485 a          172 c          202 g          396 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaa
//
LOCUS       DASJON010000338         1220 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.346, whole genome shotgun sequence.
ACCESSION   DASJON010000338 DASJON010000000
VERSION     DASJON010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.346"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1014)
                     /locus_tag="U3989_002915"
     CDS             complement(31..1014)
                     /locus_tag="U3989_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN1716319.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT          336 a          268 c          236 g          380 t
ORIGIN      
        1 acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc
       61 tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg
      121 aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag
      181 tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg
      241 aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa atgcataagg
      301 attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt cggccaattc
      361 ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt gcggagcgat
      421 ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta ctttactcat
      481 ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact
      541 gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg
      601 caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa
      661 gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat
      721 tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc
      781 tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc ttcctcgtac
      841 actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac
      901 atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat
      961 tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt
     1021 gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat
     1081 actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa
     1141 aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg tgttcaaaag
     1201 cttgcatctg cggtaataag
//
LOCUS       DASJON010000339         1191 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.347, whole genome shotgun sequence.
ACCESSION   DASJON010000339 DASJON010000000
VERSION     DASJON010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.347"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..308
                     /locus_tag="U3989_002916"
     CDS             48..308
                     /locus_tag="U3989_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716320.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(670..909)
                     /locus_tag="U3989_002917"
     CDS             complement(670..909)
                     /locus_tag="U3989_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716321.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          422 a          165 c          221 g          383 t
ORIGIN      
        1 ggtattatca ttttaaataa tggaaaaaca ttaaggatgg atgaaatatg aataacgaaa
       61 ctatcatttt aggaaaaaaa tatatttgcc aacctattgg cttgaaacaa aaagtggttg
      121 gagaagtaat cagtaaatta gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg
      181 tcgatcgcaa tgaaatatta gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt
      241 atgagttgga aacaacagaa tatctatata actatagcaa caaatatgaa catactttag
      301 tttcataaag atttaaataa agtgccttca aaagcgagaa gaccaagctt ttgaaggcac
      361 tttatttagt tggcagtgat tgttcatgat ttttgattta ttcaaaaggt tcgcctagag
      421 aatctgtctc tgtaacgagg cctggtggag aaactcgata aaaacctata ctgccagaac
      481 tgtcctattt tcggatagac aaactagtag ttttaaaaag atcatccgat ccaatcattt
      541 caaaaaatgc aaaactgata tcaggatcat ttagctcagg cttgttatcg taaagcattt
      601 gtaaaggttg catttagtta ggagtaaacg cgaaactatc atcattattt gctgaagaag
      661 atgaagaagc taattcagag tttgtttgaa ataaaggaga cgtgctgaca gtttctttac
      721 cctgtttttt gcagaggtag aaacttttat atcggaagtt tctaccggag aatttttctc
      781 atttgtagaa attgttgaat cgatactatt cgaaatttgc gtatctgaag gtatgttctt
      841 tgatgcagaa gtcccacaag cagtaagtaa caataaattt gataaactga taaacagaat
      901 tcttttcatt atgtacacac tccttatagt atcgtttagc atacacctat gctcagtatt
      961 aggcaaaatc aatgcaaaac tatcaagtct tttatagtat ctattagtct tagtaataca
     1021 tatatggaga gggtgatatc ccagttcaaa ccagatacat tggttaccat ctgtattaac
     1081 gattatgcct ctaaacgctt tttttatgaa aaaatttata agtgaaaacg taggtatgat
     1141 attatctaga ggtaattttt ttgggtattt aaatagttgc taggaggata g
//
LOCUS       DASJON010000340         1187 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.348, whole genome shotgun sequence.
ACCESSION   DASJON010000340 DASJON010000000
VERSION     DASJON010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.348"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1164
                     /locus_tag="U3989_002918"
     CDS             <1..1164
                     /locus_tag="U3989_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1716322.1"
                     /translation="GRLDDSPSHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKR
                     TRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVT
                     RILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLY
                     KLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQ
                     LRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIV
                     DELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQ
                     SGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          366 a          258 c          234 g          329 t
ORIGIN      
        1 gggagattgg atgactcacc ttcccattgt gaacactgtg gatcgattcg tattatccgc
       61 aacggttcct atacgacacg cactcagatg ttgaaagtca aagaaaagtt gactatttta
      121 gaattaaaac gtacccgttt tctctgccat gattgtggac ggaccttttc tgcgaaaacg
      181 gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcgat tctcatggaa
      241 ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc ggatgggacg
      301 gtgacacgga tcttgcgtga agcaacgaag cattaccagc cacgtatgaa cttccttccg
      361 acggtgttgt gtatggatga gttcaaatca atgaagtctg tttctggttc catgagtttt
      421 atttgtgttg atgggacaac caatcagcta tttacgatcc ttgaagatcg cagactttac
      481 aaactaactc aatacttcct gcgctttcct agaaaggccc gtttaaaggt caagtaccta
      541 gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa cagtctttcc aaatgcggag
      601 atcgtaactg accgctttca tattgtccaa cacatcaatc gcagcttcaa tcaattacgt
      661 gtccagatca tgaatcgttt tcgaacctca cactctgaaa atcagaagaa gtaccgtcgg
      721 ctcaagcgat attggaaact cttactcaaa gattccacga cattggagcc actcaaacgg
      781 cattatcatc gactgtttaa acgaccaatt agtcaaacag aaatcgttga tgagctgtta
      841 agctacaacg aagaattgcg aactgcgtat catttttgtc aattattacg ctattatttc
      901 gttaaacgag atacggaagg ctttcaagaa acccttaaaa caatttcatc tacgttacct
      961 cagtcgttta aaaagaaatt cacgatcttc catcgctacc aatcgggtat ttcaaatgcc
     1021 tttaaggtgc cccactcaaa cggcgtcact gaggggctga ataacaagat caagctgatt
     1081 aaacgaattg cgtttggtta tcgcaacttt tataactttc gggcacggat ctatttacaa
     1141 caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca cagaacg
//
LOCUS       DASJON010000341         1160 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.349, whole genome shotgun sequence.
ACCESSION   DASJON010000341 DASJON010000000
VERSION     DASJON010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.349"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..>1160
                     /locus_tag="U3989_002919"
     CDS             52..>1160
                     /locus_tag="U3989_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1716323.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTT"
BASE COUNT          351 a          226 c          232 g          351 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt
//
LOCUS       DASJON010000342         1148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.350, whole genome shotgun sequence.
ACCESSION   DASJON010000342 DASJON010000000
VERSION     DASJON010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.350"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1148
                     /locus_tag="U3989_002920"
     CDS             <1..>1148
                     /locus_tag="U3989_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1716324.1"
                     /translation="QMIWKKPKSHRTSAEEWRIASEIKGNWQDPEMKISNVLLEGDAG
                     SGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVDSNKEQDQELLEAIYQTD
                     TLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHYSYYPSEIVRAIEKGYLL
                     EIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCVIVITTNRNYQGNRPLNE
                     SLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAEIIRLLDETAKANAIKGV
                     AGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAILEQALSDSGLLLELQDIL
                     RKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEKKSLSAEE"
BASE COUNT          397 a          222 c          242 g          287 t
ORIGIN      
        1 agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag tgcagaagaa tggcggatcg
       61 cctccgaaat caaagggaat tggcaagatc ctgaaatgaa aatcagtaac gtacttttgg
      121 aaggagatgc cgggtcgggg aagacacaat tagccaaagc actatctgct gatctgcaac
      181 ttccctatac aaaagtgacc tgttttgcag atatggacaa atcagatgtt ttcggagcct
      241 tattacctgt agtagattct aataaggaac aagaccaaga attactagaa gctatttacc
      301 aaacagatac acttgaagct gtactgacac tgatccagaa ccattacggc attgatcctt
      361 tttctgctaa agaaaaatta gcagagctag ttcaaaggat cgaagctgat tatccagatg
      421 caatccatta cagctattac ccttctgaaa tcgttcgagc gattgaaaaa ggctatttat
      481 tagagattca agagccgact gtcatccgag atgcctctgt tcttgtggca cttaactcag
      541 cgctcgaacc aaatgggatg ttgaatttac cgacaggaat cattcgtcgc catccagatt
      601 gcgtcattgt gatcactaca aatcgaaatt accaaggaaa tcgcccatta aatgaatctt
      661 taagagatcg gatgcaacat gcggaaaaaa tggatcttcc tgccttagaa gtcatggcag
      721 aacgtgcgat ggcgaaaact atgatccaaa aacaagaact tctcttaaag atggcagaga
      781 ttatccgttt gttggatgaa actgcaaaag ctaacgccat caaaggtgta gcaggaatgc
      841 gttcttattt ttattgggca aatacctata agcaaggaca agatttactc cagtcgattt
      901 atccaaaagt tttgtataaa ttatccactg attcagatga attggcaatt cttgaacaag
      961 ctttgtctga cagtggattg ctactggaac ttcaggacat attgagaaaa gaaaaatggg
     1021 gtcatcctca atctgagcat acaaaagaaa gaaccattag cgccgaagaa gcaaatgaac
     1081 gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga tgaaaagaaa tcgttgtctg
     1141 ctgaagaa
//
LOCUS       DASJON010000343         1139 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.352, whole genome shotgun sequence.
ACCESSION   DASJON010000343 DASJON010000000
VERSION     DASJON010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.352"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..969
                     /locus_tag="U3989_002921"
     CDS             <1..969
                     /locus_tag="U3989_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1716325.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          387 a          199 c          228 g          325 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatgg
//
LOCUS       DASJON010000344         1073 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.354, whole genome shotgun sequence.
ACCESSION   DASJON010000344 DASJON010000000
VERSION     DASJON010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.354"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1073
                     /locus_tag="U3989_002922"
     rRNA            <1..>1073
                     /locus_tag="U3989_002922"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DASJON010000345         1070 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.355, whole genome shotgun sequence.
ACCESSION   DASJON010000345 DASJON010000000
VERSION     DASJON010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.355"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            116..808
                     /locus_tag="U3989_002923"
     CDS             116..808
                     /locus_tag="U3989_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1716326.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          463 a          153 c          179 g          275 t
ORIGIN      
        1 gagtatttga tagtatttat attaaaatta catatgtaat ttaaaaacgc ttgacattgt
       61 gtttgagatc acttactttt agcatgtaaa agtattggat taaggagcta tgcaaatgaa
      121 aaagaaattt attactggtg ggattgtcgt tgcatttcta tgtctaattg gctacacagg
      181 cacgcggtac gcaaatgaaa ttattttgtg gggtggagag acagatattg atacaatcaa
      241 tgagaatctg acacagcttg atcatacatt gacagataaa gagcaagcga ttacagatct
      301 taccgctcga ctagcacaaa aagaacagac attgcaagat tcaaaggcag atgtccaaga
      361 ataccaaaca aaagtaaatg aattagaaaa ccaaaaaaat caatcggcag cagaaaaaca
      421 aaacttagaa agtcaattag tcagcaagga tgcacaacta caaagcaaac aaaatgaaat
      481 caatgtcaaa ctagatgaga tcaaccaaaa aaatgatgaa atcaatcatc tgaatgcaga
      541 ttggacagca agactacaag aagcacaagc aaaactttca gaattacaaa atactgtaaa
      601 tggtttgaat caacaaatcg caacactgac acaagaaaaa acaaatgtag aaactcagtt
      661 aacagataca caaaagaaat tagacgaagc aactgctgaa aactcaagct tgaaacaata
      721 tattcaaaag ttagaaaaag caaaagatga agtggaagat acagctgata aatctcagca
      781 aattgttgaa gaacatttgc gtaaataatc tttcttaaca agtaaaccta aattagtaaa
      841 gaagcaacag taatctatac taagtagttc agttttggag tacaaaatga caaaatgtac
      901 ttcaaaatgg actacttttt tcattttaac ttacatacca aattacatac aaaatgattt
      961 ataaaatgca attgaaaatg ataattgaat gaaaatttgt gattttgtgg tattatgata
     1021 atataactta tagaaaaaat tgcttattca actaacgggc aggataatga
//
LOCUS       DASJON010000346         1061 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.356, whole genome shotgun sequence.
ACCESSION   DASJON010000346 DASJON010000000
VERSION     DASJON010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.356"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..992)
                     /locus_tag="U3989_002924"
     CDS             complement(36..992)
                     /locus_tag="U3989_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716327.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DASJON010000347         1047 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.357, whole genome shotgun sequence.
ACCESSION   DASJON010000347 DASJON010000000
VERSION     DASJON010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.357"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(857..>1047)
                     /locus_tag="U3989_002925"
     CDS             complement(857..>1047)
                     /locus_tag="U3989_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1716328.1"
                     /translation="WLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLM
                     LNEQNIVLATHPKYFKNI"
BASE COUNT          394 a          129 c          163 g          361 t
ORIGIN      
        1 ggcagtggta tttcttcagt aagattaaaa gagaaagaat atactcaaaa gattattcat
       61 attcctaagc ctgaaattaa gcgttttgag ataaaagact attctgcttt agggctgttc
      121 tcaattggtt actggtgata aaaaataaga atactaaaac tatacgtaga atataagtaa
      181 tcggcatcaa tcctaattat gtgattgaat gccgattttt gttacgcgct atttttaatt
      241 atctgtaaga agaataattt tattttactc atattcgttt tcactttgaa gaagtaaatt
      301 gcaatttctt ataagttaag cagctatgca aaaggctatt tattctattg tgaaatataa
      361 gatgtagttg aaacaggata tatagaatca aaatacgaat cttattttga tttttctagt
      421 tttttgaaaa aagcaccctt tgatcgttgt attagtggaa aagagaaaaa ggagcatact
      481 tatcatgtca caaaactaca actaaatatc taaacaacac caatttgatg cctattgaaa
      541 aaaagtattg cgaaatgaag ccaagtctat tagaaaatga aataatagaa taaaagaaaa
      601 tgggaaaccg ctgaatgatg taaatgaagg taagtatcca cgaactaatt ttgatgaaca
      661 agatatctat tttttatttg tattggagat actagtttaa tatcaaaagt tatcagcagt
      721 cataattagt tatccaatgt aaaaagtaca aacaatagtt gttatgcaga atagtttatg
      781 aaatatatta cataataaga aaacaagcga tatttttctg atcagaacaa ccatttaatt
      841 taaaaagagt taaaagttat atatttttga aatattttgg atgagtagct aaaacaatat
      901 tttgctcatt aagcattaaa tgaagatgca acgatgttag aaagttagct tcttctggat
      961 cttgttttgt taatgcctca aatagttgat aatgttgttc cataattttt tcccacttta
     1021 atccttccgt ttttactcgt agccatc
//
LOCUS       DASJON010000348         1043 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.358, whole genome shotgun sequence.
ACCESSION   DASJON010000348 DASJON010000000
VERSION     DASJON010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.358"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            142..555
                     /locus_tag="U3989_002926"
     CDS             142..555
                     /locus_tag="U3989_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN1716329.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          355 a          156 c          172 g          360 t
ORIGIN      
        1 tttcaaatta gaaagatagc ctttgtatcg tattagatga aatattcaca aagctaacca
       61 ttatacttgc atttttttca aaatagagtt attacgtaaa tagggataaa taaaaaatac
      121 ttattgaaag gatgatcaaa aatggcaaat gtacctagca ttcgcgacat gttccctgat
      181 ttcaatgacg tgtttagtcc agcattcaat gattttttag gtgtttcaag ttatccaaaa
      241 gtagatttag tcgaaaatga aaaagaatac aaactaactg ctgatatgcc tggctgtgac
      301 aaagaagaca cgacagttga atattcagat aatactttga cgatctccgc caatcatgag
      361 tcccatacag aagataaaga agatggcaac tatgtacgga aagaaagaca ttcggtctct
      421 tacaaacgtt cattctatct tcctaatgtt gatgaagaaa aaatcactgg aacctttaaa
      481 aacggtgtgc tgaaattggt attacctaaa acagcttatc agccaaaaga aacaaaaaaa
      541 attgagttaa attagaactt atcgcctctc gatgaatgag gggcgatttt tatatatttg
      601 gataagaacg agtatgttta aattttcaaa atgagcaaaa aaatattttg tttttacgat
      661 aaagatgtta tgatctctct tggtaaaaaa tatgggagtt gatcatttga ttgtcttaag
      721 taaacttttg ttttggatac catttatcat aatgaccagt gctttctttt acttattcaa
      781 aaggcctaac aaatacttac tcttatctgc ttgtccgatg atctatttta tccataagat
      841 tagaggattt tattattacg actctggtat ctcattgttt tgctacgaaa tcggcttatt
      901 agcttcggcc atctttttca tatttgtgtt agaatatttc agaaaaaaat aaacttccag
      961 tcagttttgg ctggaagttt tttatttggt gcagatcttt tcagctgtta ttcatacaag
     1021 ttgatccatt tttctattat taa
//
LOCUS       DASJON010000349         1042 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.359, whole genome shotgun sequence.
ACCESSION   DASJON010000349 DASJON010000000
VERSION     DASJON010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.359"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..941)
                     /locus_tag="U3989_002927"
     CDS             complement(<1..941)
                     /locus_tag="U3989_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN1716330.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILS"
BASE COUNT          411 a          168 c          127 g          336 t
ORIGIN      
        1 atagatagta taaaaattaa aacaatacca agaaacgcat aattcacttt acttctaaaa
       61 aatgttttga gtaaactaaa gatatttaac acatagaaag catagataca tataaaagca
      121 attataccac ttgatacaag tacttgaaga aatccattat gcatgtttcc atgtgctctt
      181 tttagttcat tcacatctgt agtatcaaga tctacttttt tcaaaaattt tgcatcgcta
      241 ttcccataga aatcggtctc tccataacca aataacaaag ttggtcttat aactttaagc
      301 gcagctttcc aaatcgtaaa acgaccagct gaaatttcgg agttatcact agaatgctgt
      361 aatattactt ctttttctgg aagtggagtt ttatctgatt catttgactc tataacttct
      421 acttttctat caatcgatac aggaattgtt gctaaaaaat ccttagcata attagtacca
      481 aaagaaaaat ataccaatag aactaataat atgcttacca tccatttgat ttttacaggt
      541 agtacttctc taatgctctt tctaaatttt gaattggcaa acaacaaaag aaataatact
      601 aaaaaagaac aaattactac ctgggcacct ctagacgaag aaagtgtaat ataaaatatt
      661 tgtacgataa aattcatcat ataaaaaaca cgaatcgttt ttgttatttt tgaaattaat
      721 acactaatta aacttaaaat aatagaagta tatccaaaca tagctccaac atttggactt
      781 gtatagacac caaataaccg atgttcaata aaaccttgtc ttgcaagtaa tcctggcgtt
      841 cctgttggga catgataaga aatcaaaaaa ataaattgat agatagatat tagcgcagct
      901 aaaaaaatta tgataatgaa aacattatta aaatacacca tttttttctt tttttcctct
      961 tcactttgtg aaaagtctgc aggaaaaatt actatcaaag taatcagcaa atatccaaaa
     1021 ttataaatag atggaattaa aa
//
LOCUS       DASJON010000350         1042 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.360, whole genome shotgun sequence.
ACCESSION   DASJON010000350 DASJON010000000
VERSION     DASJON010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.360"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..695
                     /locus_tag="U3989_002928"
     CDS             288..695
                     /locus_tag="U3989_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716331.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..>1042
                     /locus_tag="U3989_002929"
     CDS             709..>1042
                     /locus_tag="U3989_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716332.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLD"
BASE COUNT          390 a          130 c          179 g          343 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga ta
//
LOCUS       DASJON010000351         1040 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.361, whole genome shotgun sequence.
ACCESSION   DASJON010000351 DASJON010000000
VERSION     DASJON010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.361"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1040
                     /locus_tag="U3989_002930"
     CDS             <1..>1040
                     /locus_tag="U3989_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1716333.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQ"
BASE COUNT          363 a          204 c          187 g          286 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa
//
LOCUS       DASJON010000352         1009 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.362, whole genome shotgun sequence.
ACCESSION   DASJON010000352 DASJON010000000
VERSION     DASJON010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.362"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..497)
                     /locus_tag="U3989_002931"
     CDS             complement(<1..497)
                     /locus_tag="U3989_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEN1716334.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          346 a          160 c          165 g          338 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctcttttgt tactatgaat aacataataa gaaagtaaag tggattgatt tgagatcttt
      661 ttatacttca aaaaagataa cacccaactt tcaaaaatat gttttggaag ttgggacaaa
      721 cgagttaata cattttaata cattattttt tattcaaaaa aaatagcaca atggcaaaga
      781 tcgtaagaga gatcataagc cccaatgaat gactaaaaag aaaagcagaa tcacatgcca
      841 tatcatccca tatttcttca agaaaataaa taagcgggaa aaagggaagg aatagtagaa
      901 catcgatcca gcaccatttt tttcttcttt tgaaataaca taaaacaata aacatgaacg
      961 tgcagggaag taaaaacaaa gctttactca taactaacaa aataccagc
//
LOCUS       DASJON010000353         1004 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.363, whole genome shotgun sequence.
ACCESSION   DASJON010000353 DASJON010000000
VERSION     DASJON010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.363"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..261
                     /locus_tag="U3989_002932"
     CDS             <1..261
                     /locus_tag="U3989_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN1716335.1"
                     /translation="EDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRH
                     TNKDVHDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(407..883)
                     /locus_tag="U3989_002933"
     CDS             complement(407..883)
                     /locus_tag="U3989_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN1716336.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
BASE COUNT          315 a          182 c          147 g          360 t
ORIGIN      
        1 gaagataaaa taaaaaaaat gttattaggt aaaacaattg aaaattattt gaatgatttt
       61 cctaaactcc aaagattagc caaagctgct acttggattg gtaatgatga aacgcactat
      121 gttcgtagac atactaataa agatgttcat gatatggatg catttattaa atcagcagcg
      181 caatttatcg cggctgatta tgatgctgat ttagctgatc aatttataaa tggaaataac
      241 tttatgccaa ctactaatta aaagaataaa agagccacag ataccaatta ataaaacggt
      301 tgggatatct tggctctttc atattttttt gacactaata gaatatcatg ctgctaacaa
      361 tgtttacata gctacaatga ccgaacattt agtgaacagt gtaattttag aataaagaga
      421 gttgttcttt gtacttctcg ctattgtaaa aatcaatttc gttttctttt ttcaatcggt
      481 cagcttgtct gtgttcatac tcatctaaaa agttcaatgt ctttctaatt tctgcatgtc
      541 gctgtctgac atacgatgag ctatacccaa cagattcagc aatatcttct aagctcattt
      601 gatcaacata tttcatctta acaatttgat tatcgatacc agaaaagcta tctatcagca
      661 ataacatttc ttctttttgc tcgatcaaca attccaattc attttctatc ttttggatat
      721 tctcttctaa agaagaagtt ctcgaattct tttcgatgcg gacgtttgct aaatcaccac
      781 agacccatcg attcaattca agcttacttt tattaagatt ccactttaag taaagaatct
      841 gctcatctag ttcttggtaa tcttttaacc aatgaaatct cacaaacgcc accccttatg
      901 gtagaatagt attgcgctgc tatctcgaaa ggggtagctt ttttattttt tcttagccgt
      961 tccattctgg attgcctttt gttccaaacg tcgttttttc ttct
//
LOCUS       DASJON010000354          961 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.364, whole genome shotgun sequence.
ACCESSION   DASJON010000354 DASJON010000000
VERSION     DASJON010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.364"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..459
                     /locus_tag="U3989_002934"
                     /pseudo
     CDS             172..459
                     /locus_tag="U3989_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="U3989_002935"
     CDS             473..904
                     /locus_tag="U3989_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716337.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DASJON010000355          948 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.365, whole genome shotgun sequence.
ACCESSION   DASJON010000355 DASJON010000000
VERSION     DASJON010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.365"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..480
                     /locus_tag="U3989_002936"
     CDS             <1..480
                     /locus_tag="U3989_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716338.1"
                     /translation="EVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIK
                     LHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSI
                     MLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            467..>948
                     /locus_tag="U3989_002937"
     CDS             467..>948
                     /locus_tag="U3989_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1716339.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     "
BASE COUNT          309 a          126 c          133 g          380 t
ORIGIN      
        1 gaagtttttg tcggagataa atgtatcttt tttgatcaac ctttattatc tgatggttat
       61 ggcatagatg aaacagatat tttcgctaaa gtaaatagta tattttctgg aaaaaaagaa
      121 actttaatta agcttcatcc tagagcaagg acaaatcgtt atggtgaaaa aaacatattg
      181 aattccagtt taccgtttga agtcttgcta cttgaaaatg acttctcttc aacacttttg
      241 attagtccta tatctacaat ttctttttca ccatctatga tgtttgaaaa gacgatcaac
      301 tctattatgt tggctaaatt aattttaaat gaatatacag cattatctga agataaaaaa
      361 gaaacattga caaatattgt tcttttctgt gaacattata accgtctatc agaagaaaaa
      421 attttactcc cagaaaactg ggcagaatta agaaagttgg tagagtatgg gattatttaa
      481 cacactttcc aagtcaaaat tagttaagtc cagtttctgg tatactatag ggaatttttt
      541 tattaaggga atttcattca taactattcc tatttttgta aatattacta ctcaagcaga
      601 atatggtttg attaataatt tcacttcatt tgtatcaatt ttttcgattc ttgttgggct
      661 aagcctaaat tcggcaatta ataatgcaag ttttgactat aaagatgaca taaatgaatt
      721 tatgtcatcg gtactttctc tagctacact ttcgttaatt gtgttcttat taatagggaa
      781 catatatttt ttgttttcaa atgctttttt tgatatgaac caacttgtat ttaatttgat
      841 gttattacaa agttttgcta gttttgtaat tagttatatc cagtcttatt ttacgattaa
      901 ctttaaccat tttaaatatt tgattttatc ttttatatct actttttt
//
LOCUS       DASJON010000356          934 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.366, whole genome shotgun sequence.
ACCESSION   DASJON010000356 DASJON010000000
VERSION     DASJON010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.366"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..423
                     /locus_tag="U3989_002938"
     CDS             <1..423
                     /locus_tag="U3989_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN1716340.1"
                     /translation="RWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAE
                     LTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNH
                     EVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            423..>934
                     /locus_tag="U3989_002939"
     CDS             423..>934
                     /locus_tag="U3989_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716341.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          308 a          145 c          232 g          249 t
ORIGIN      
        1 cgttggagac aagaacagga acacagtttc caagattgga gagaaggaca agaatcagac
       61 tatttggcat ggtttgaatc aatcaaggat attttaaaga cgattgatcc aggtggagtc
      121 atgttagccg aactaacgga tgcgcgagta gatattcaag gagttcgtca tgcgtctatt
      181 tcagagcgtt tgctggcaga tatggactat ttatatcaga aattgcgagc aacgcttttc
      241 acgattgaat acggggagat tgaagtgact gatattttgc aggatgatct cttttcagat
      301 aatcatgaag tcgaaaaagt tggaactgta gcattcccga ttgaagaagg agccttgatc
      361 atcgcaaccg ttgatgatcc aaagcaaaat gtgttcactc ttgagaaagt tggggtgatc
      421 taatggctaa aactaaacga atgatggaaa cggatgaaaa aacgggtgta caacgccaat
      481 tctttccaat cacacatgct tccgcagttc ttgggttaga agaaataata gcaggagaag
      541 caacagtttt atctgttaat ggaaaaattg gagccgtcgt cattactaaa gaagatttgg
      601 gattagagaa tgttctcaca gaattaccct atgcaagtga agaagatgac ggcattatca
      661 cggcagaaat gtatcaaaaa attttaaaca gtggagaagg tgactacgtg ttgccagttg
      721 ccactatcga ccgtttgggt ggcataaaag ttggtgaatt attgacgatt gatgagacag
      781 ggaaagtctc tgcagtcaga caatctgatg tcaatttttc gctggagtta aaagaaaaac
      841 tcgattcact gaaaaattat actgctggag aaaacatcac tattgatgaa gatggaacaa
      901 ttagttctac aggtggtagt gggactggtg gtgt
//
LOCUS       DASJON010000357          931 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.367, whole genome shotgun sequence.
ACCESSION   DASJON010000357 DASJON010000000
VERSION     DASJON010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.367"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..423
                     /locus_tag="U3989_002940"
     CDS             <1..423
                     /locus_tag="U3989_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN1716342.1"
                     /translation="RWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAE
                     LMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNH
                     EVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            423..>931
                     /locus_tag="U3989_002941"
     CDS             423..>931
                     /locus_tag="U3989_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716343.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          323 a          124 c          209 g          275 t
ORIGIN      
        1 cgctggagag aagaacagga gaatagtttc caagattgga gagaaggaca agaatcagat
       61 tatttgtcat ggtttgaatc aatcaaggat attttgaagt ctatcgatcc aggtggagta
      121 atgttagccg aattaatgga tgcacgtgta gatcttcaag gtgtacgcca tgaatcgatt
      181 tctgaacgtt tcctatccga tctaaattat ttgtatcaaa aaatgaaagc agcacttttc
      241 acgattgaat acggtgaaat tgaagtgact gatattcttc aagacgatct cttttcagat
      301 aatcacgaag tcgaaaaaat tgaaacagta gaatttccaa ttgaagaagg agctttgatt
      361 attgcaactg ttgatgatcc aaaacagaat gttttcactc ttgagaaagt tggggtgatt
      421 tgatggttaa agtaaagaga atgatggaga ctgatgaatc tggtgttaaa cgtcaattta
      481 tgccaattac acatgtttct gcagttttag gcttagaaaa aatgatatca ggacaatcaa
      541 aagttctttc ggttaatggt aaatatggtg cagtcatttt aacgaaagct gatttaggat
      601 tagagaatgc aattactgaa ctaccttatg caagcgagac aagcgatggt attcttactg
      661 ctgaaatgtt tcaaaagatc gtaaatggag aaggtggaac atatattttg ccgattgcta
      721 ctcctgaaca attaggaggt ataaaagttg gggaactctt agaagttact gaagaaggtg
      781 ttttatctgc aactaagcaa acagacttca atttctctga agaattaaag ggaaaattag
      841 aatctctaaa aatattaaaa gcaggtgcaa atatatcaat tgcagaagat ggaacaatta
      901 gttctacagg tggtagtggg actggtggtg t
//
LOCUS       DASJON010000358          910 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.368, whole genome shotgun sequence.
ACCESSION   DASJON010000358 DASJON010000000
VERSION     DASJON010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.368"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..355
                     /locus_tag="U3989_002942"
     CDS             <1..355
                     /locus_tag="U3989_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716344.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="U3989_002943"
                     /pseudo
     CDS             615..910
                     /locus_tag="U3989_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DASJON010000359          899 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.369, whole genome shotgun sequence.
ACCESSION   DASJON010000359 DASJON010000000
VERSION     DASJON010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.369"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            357..473
                     /locus_tag="U3989_002944"
     CDS             357..473
                     /locus_tag="U3989_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1716345.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          309 a          146 c          144 g          300 t
ORIGIN      
        1 aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac
       61 gcttctgtcc cagcctcttt tttagtgttc tatcgaacac acagtacgtt caataggtaa
      121 actagctata acaatagata ttaaagttaa cggacaagag ggcctttttt aggtagctaa
      181 aacgtaataa tgaaaattta tttccagaga taggagtcaa tcagctagtc tgatcgactg
      241 ttttaacaag ttgactttca ttttaacttt tgataccatt taaaacacaa atcgtaattg
      301 tttcgatttg tgttttttag catgactaca atcaaagaag aaagtgggtc gtccatatgg
      361 aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag cccaaatatc aaaacacgaa
      421 aacgtgcgct gaagatcatc aaagatgtga aacggaatag tgggaaaagg taagaagaag
      481 gaaacttaat taaaaaaata gccgaaaaac gtttttcggc tattttcaat aatcggtttt
      541 tgtgtaaggt ttccgaaaga actgctgttg attttccatt tttaacatat gataaattgg
      601 tagaatcttt cttttcaaat tgataccatt ccctaattcc agttatatag ttaactacag
      661 aaccacaaag cacgtacgtt tataaaagta ttatctgtag ttatttttca attccctttc
      721 aacctcttat tttgaccagt agtattgatc actttacctt ttaacacctt tgttcgtttt
      781 tgaaacattt ttagtcaaga atggaaacgt aactacataa tcaaaggttt tgcgaaatat
      841 ttcatgaaat atttcatgta gaagcgagca atacggttgc aaataaccat tatcgttta
//
LOCUS       DASJON010000360          890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.370, whole genome shotgun sequence.
ACCESSION   DASJON010000360 DASJON010000000
VERSION     DASJON010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.370"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..329)
                     /locus_tag="U3989_002945"
     CDS             complement(<1..329)
                     /locus_tag="U3989_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1716346.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTL"
     regulatory      complement(403..507)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(570..>890)
                     /locus_tag="U3989_002946"
     CDS             complement(570..>890)
                     /locus_tag="U3989_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1716347.1"
                     /translation="PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPV
                     AKAELTFDQQNYLIGAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLS
                     YLLK"
BASE COUNT          290 a          187 c          162 g          251 t
ORIGIN      
        1 catagggtaa gtaatggccc taacaccatg attggcaaca tttgtgaaat cactagtaac
       61 ggataaaaag acatttctaa aaacttccaa taatccattg ccaaggcagt aataaaagaa
      121 agaacgctag ctattaccaa accaagcact gcttcattta atgtcgtgac agaatgggaa
      181 agtaatactt gtgcatctgc cgctaatgca gaaaatacag cagcaggtcc gggtaaaata
      241 aaggcaggta ttttccaaac tatgacaaca atctgccaaa taattaaaat gaatgccata
      301 ctgactgctg gataaagatt tttcttcatt ttgttacctc ctttcttcta atatgaataa
      361 caaaaaacgc ttgaaatggg cacgacatag taacccgctt caagcgtctg ctatgtttct
      421 cccttcacca gtattaactg acaggttcaa tgggtctaag ttcacaactt actctcatgt
      481 cgtctacgac atccccagaa acaggtattc attttttaaa gtgtagcaca agaaggaaaa
      541 agcaacaagc gctttagtta tcatgctgat tatttcaata aataggagag atcacttgcc
      601 aatgtcggat accccatgat atagctatcc aactcttttt tagtcaattt ttgattgatg
      661 acgagtgtca aatcatcgat caattcgtct gcttgctcgc taatcactgc agcaccaatc
      721 aaataatttt gctggtcaaa ggtcaattca gccttagcaa ctggttcatt gacacgatga
      781 taggtgaacc aactggtaag atccatctct accacttgat ctgacgaaga ggcgtgactt
      841 tttgtaactc ctacttcagc aagtttagga ctggcgtaga cgatcgtagg
//
LOCUS       DASJON010000361          877 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.371, whole genome shotgun sequence.
ACCESSION   DASJON010000361 DASJON010000000
VERSION     DASJON010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.371"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..350)
                     /locus_tag="U3989_002947"
     CDS             complement(<1..350)
                     /locus_tag="U3989_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716348.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMY"
     gene            complement(357..>877)
                     /locus_tag="U3989_002948"
     CDS             complement(357..>877)
                     /locus_tag="U3989_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN1716349.1"
                     /translation="LGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNKLVDELTFYFD
                     TVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKVDWKRYAGCVA
                     INSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAAKELAKVNIVA
                     GIAAAAASCALE"
BASE COUNT          295 a          181 c           91 g          310 t
ORIGIN      
        1 aaatacattc ttttccagtt acatttaatt tttaaacaaa attctcccca aaaaggaact
       61 ttttctagtg aaaacttggt aatctctttg taattaattc ttgttaaaga ataattaaac
      121 acttcatttt tacttgttat tttttcattg actataacta gttcatctgg taaaaaaatt
      181 aaccaacaaa catcgacaaa cagtaaatga tataatccat ttccacctcc aagcatattc
      241 aatactggtg aacatgcctt agaaacacga atatagttat ggtttaaata gatattttct
      301 ttaaacattg cttgtttaaa agccatatgc tcttgttcta aacttttcat aaacttttac
      361 tctaatgcac agctagcagc agctgctgca attcctgcta caatatttac cttagctaac
      421 tctttagctg cagcttttcc taattgtttt tctgcaattt ttaataataa atccgcagct
      481 ttttcaaaat taaaagcttt taatgcttca ataaaatctc cctgcgtacc tatagcccaa
      541 gccactcctc ctccaggaag agaattaatg gctacacatc cagcatatcg tttccaatca
      601 actttctcca ttctaggaac aataatatgt tcataaaaga ctcttgctgc tcttccttgt
      661 tcgttatctt gactgattcg ttcacgaata gcaccaaaat cctttatatg gtatacacca
      721 tgttcgtcta aaaaaccaac tgtatcaaaa taaaaagtta attcatctac taatttattc
      781 tttatttcta aatcaactgt attcactttt tctgtggcat acacacttgt tccagtacta
      841 acaaaagtac ttaaacctaa taagaaaaca cctaaaa
//
LOCUS       DASJON010000362          833 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.372, whole genome shotgun sequence.
ACCESSION   DASJON010000362 DASJON010000000
VERSION     DASJON010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.372"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>833
                     /locus_tag="U3989_002949"
     rRNA            <1..>833
                     /locus_tag="U3989_002949"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          207 a          203 c          249 g          174 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct acaatgggaa gtacaacgag tcgcgaagtc
      661 gcgaggctaa gctaatctct taaagcttct ctcagttcgg attgcaggct gcaactcgcc
      721 tgcatgaagc cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg
      781 ggccttgtac acaccgcccg tcacaccacg agagtttgta acacccgaag tcg
//
LOCUS       DASJON010000363          826 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.373, whole genome shotgun sequence.
ACCESSION   DASJON010000363 DASJON010000000
VERSION     DASJON010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.373"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>826
                     /gene="holA"
                     /locus_tag="U3989_002950"
     CDS             <1..>826
                     /gene="holA"
                     /locus_tag="U3989_002950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN1716350.1"
                     /translation="LPFFGDQRLVFVENPYFLTGEKVNNGIEQNTDLLTAYLKEPLES
                     TVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYLMNTLENSSIKMERSA
                     IDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPKTLEHNIFDMTQYILK
                     GKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKIGYQQANIAETLKIHP
                     YRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSFQLFVLRTGQLLRNR"
BASE COUNT          313 a          115 c          156 g          242 t
ORIGIN      
        1 ttgccgtttt tcggagatca acggttagta ttcgtagaga atccttattt tttaacaggt
       61 gaaaaagtaa acaatggaat cgaacaaaat actgatttat tgacggctta tttgaaagaa
      121 ccattagaat cgactgtatt agtattcttt gcgccatatg aaaagttgga tgaacggaaa
      181 aaagtaacga aacaattgaa aaaaacagca attatcatca atgtccaaca gttgaatgaa
      241 aaagaagtac gtcaatatct gatgaatacc ttagagaatt cctcaatcaa aatggaacga
      301 agtgcaattg atttattctt gaggttgacc gacttagatt tatccaaaat gatgagagag
      361 ttacagaaat tggtccttta tggacaaaat caacagcaaa tcacggtaag agaagtagaa
      421 gaattagttc ctaaaacgtt agaacataat atttttgaca tgacccaata tattttaaaa
      481 gggaagacgg aacaagcttt gcgtttatac gaagatttag ttatgcaagg ggaagaaaca
      541 atcaaaatta atgcgatttt gctttctcaa ttacgtttat ttttacagac aaaatttctt
      601 gtaaagattg gttatcagca ggcaaatatt gctgaaacat tgaaaatcca tccatatcgt
      661 gtaaaacttg ccatgcagga agtaaaaaaa ttcgatgaac atttgctggt acagttgttt
      721 gatcaattag ttgagatgga ttatcaaatc aaaacgggac aaatcgaaaa agaattaagt
      781 tttcaactat tcgttttgcg cacagggcag ttgttaagaa atcgat
//
LOCUS       DASJON010000364          811 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.374, whole genome shotgun sequence.
ACCESSION   DASJON010000364 DASJON010000000
VERSION     DASJON010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.374"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..672
                     /locus_tag="U3989_002951"
     CDS             <1..672
                     /locus_tag="U3989_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1716351.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DASJON010000365          807 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.375, whole genome shotgun sequence.
ACCESSION   DASJON010000365 DASJON010000000
VERSION     DASJON010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.375"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(167..541)
                     /locus_tag="U3989_002952"
     CDS             complement(167..541)
                     /locus_tag="U3989_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1716352.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(564..>807)
                     /locus_tag="U3989_002953"
     CDS             complement(564..>807)
                     /locus_tag="U3989_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HEN1716353.1"
                     /translation="NYPWPQMFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVAL
                     STRMYDKVGTALSMTVTEGAIKVDKLDYSINYECGF"
BASE COUNT          265 a          160 c           88 g          294 t
ORIGIN      
        1 attccaataa ataggaagga ttacaaaatt tgaaaccaaa taagtaaaaa atactgtcac
       61 tttgaattaa tttgaaatag taaattctca ttaaactatt gaaatgctga taccaaaaaa
      121 tgaagtactt ttcgttcata ccaacttaga gcacttcgtt atctatttat ttcttcattt
      181 ggcttatctc tataatctca tgatttggtc cgtagaaatt aaagtaacgg attccatttt
      241 cccagaacgg taattccata atatttttat ctaataattc atatcctaaa tcttgaattg
      301 aattgaatac ctcatctatg tcatccgtat ctaatgctag atgatcaatg gctcctctac
      361 actcagtagt tttatcttcc catatttcta ttatcgttcc attcaactcg aaaaagccga
      421 ctttacttcc cacattcatt tcttcattaa tcagactaaa ccctaaggat tcataaaaga
      481 tttttgaagc tccaaaatct tttgttggaa ttccaatatg ttgtaaacca tttaatttca
      541 cttacatcat cccttcaatt gttttaaaat ccacattcat aatttattga atagtctagt
      601 ttatctacct tgattgctcc ctccgtaaca gtcatactta atgctgttcc gactttatca
      661 tacattctag tacttaaagc aacattatca atatataaaa cagctatcgt atcatcaaca
      721 attaacttta gttcatattc tttgcctatt tccaaaaata gtggtcgttg cagcccctta
      781 tcaaacattt gaggccaagg atagttc
//
LOCUS       DASJON010000366          805 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.376, whole genome shotgun sequence.
ACCESSION   DASJON010000366 DASJON010000000
VERSION     DASJON010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.376"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..496
                     /locus_tag="U3989_002954"
     CDS             <1..496
                     /locus_tag="U3989_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1716354.1"
                     /translation="LVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYIL
                     FTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESKE
                     EQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNVS
                     IVES"
BASE COUNT          276 a          153 c          141 g          235 t
ORIGIN      
        1 gttagttatt attgatatgc aaaatcatat tcttgatcca aacagcgaat tttatattcc
       61 agagtcagat caattggcag aacgtattgc tcaaagatta gctaaagcac gccaaaataa
      121 tgaatatatt ctcttcacac gggatatacc agttgaactg aaggacaaag aagaagaacg
      181 agaagatttg caattgatcc ctcttctttc tccacaagaa aatgaacgag tgattaaaaa
      241 gtattacttt acgattccac ctgaaacttt agctgaaatc caggaaactt tatttgaaag
      301 caaagaagaa caaaaagaga tcgaagtagt cgggattgaa acaaacttgt gtgtactctc
      361 taatctgata ggcctgcaga gtgcttttcc tgaagcagac ttttttgttg atccaatgtt
      421 agtctccagt agaaaacacg gagaatccgc tttagagctg ttgaaagatt tcaacgtctc
      481 tatcgtcgaa tcataaaaat atctatcttc atttacgata tttaacacac aataaaaaaa
      541 gcacgcagaa ctgttttagt cctacgtgct ttttctatca aagtagattg gcttttttat
      601 gacacagagt gaaacggcag gctcacgacc tctttcttaa atggtgttca atcagctgac
      661 cctcggtatt agcttaacaa acggcaaaat atgaggaact atttttccgt ttgttaagct
      721 aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtatt caaaagcttg
      781 catctgcagt aataagaaca gccaa
//
LOCUS       DASJON010000367          804 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.377, whole genome shotgun sequence.
ACCESSION   DASJON010000367 DASJON010000000
VERSION     DASJON010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.377"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            14..430
                     /locus_tag="U3989_002955"
     CDS             14..430
                     /locus_tag="U3989_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEN1716355.1"
                     /translation="MMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIP
                     ILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYIT
                     KTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(662..>804)
                     /locus_tag="U3989_002956"
     CDS             complement(662..>804)
                     /locus_tag="U3989_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716356.1"
                     /translation="HNLKKWLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDL
                     LL"
BASE COUNT          309 a          104 c          104 g          287 t
ORIGIN      
        1 gattggagag aaaatgatgg atcaaaaagc gtttatcgaa tctattgatt ctacaatcga
       61 tagattaaaa aaagaaattc gattttataa tagagtaatt ggtatcggaa atattataaa
      121 aatagtctta tcagctagta tcccaatatt aattgatcag gcttccgaac acaggtcttt
      181 actattgatt gtttcaattg cttcagcaat aattacaatt atacaaagtg ggatgtctgc
      241 atttaattat caagataaag tacagacttc gacagagtta ttaatgaaaa ttgaaaaaga
      301 aaagttgtta tatataacga aaacatcccc atataataaa acagatgaag aaaatttcca
      361 tttaatcgtt acaacccttc aaactgaatt aaatgatatt atctctgatt ttaatcaagt
      421 taataactaa aaatttattt aattagaaag tagtgcagat aatactaatt gtacaattaa
      481 gaaaagagat aagactttac attcctaaaa aaatgtaaag tcttatctct tttcttaatg
      541 ctataaattc ataataacat tatggacaat aaatttaaaa tttattcaga ctgtagacaa
      601 actcctaaat ttagagtgtt gtttgcagtc tgacgactca aaaatgagtc gtataaatca
      661 attatagtaa taagtcattg atttctttaa attctttgtc aagttctttt tcatcatatt
      721 tttcatattt atcagcttca tgaagaactt ttttaatttc atgaatagcc tttcgttcaa
      781 ctaaccattt ttttaggtta tgtt
//
LOCUS       DASJON010000368          792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.378, whole genome shotgun sequence.
ACCESSION   DASJON010000368 DASJON010000000
VERSION     DASJON010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.378"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            144..>792
                     /locus_tag="U3989_002957"
     CDS             144..>792
                     /locus_tag="U3989_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1716357.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGN"
BASE COUNT          277 a          137 c          138 g          240 t
ORIGIN      
        1 ttatccttac tttgaacatt aatttcacca aaccgaatga taacaagtgt ttttctgaac
       61 ttttttacca gaatagctat tataaatact ttctgcgctt ttctaggata tactgaaaac
      121 atcaataaaa ggaggcacca ctcatgctta caatcgctta tcttggcaat ggaaagagta
      181 caaatcgcta tcatttgcct ttttcaagaa aacttacgaa caaaatcaat atcaaaaaaa
      241 ttttttcccc atctgaacca gtgtggaaaa cattcgaaga catcgaatat actgatcagc
      301 tgtctgatct ttgggatgat cctgagatcc aattagttgt catcactaca cctagtcaat
      361 ttcattatga atatggaaaa atggcactcg aacatggtaa gaatgtacta gtagagaaac
      421 cttttgcaga aacgtcaaag gaagcagaag agttatttgc attagcaaaa gaaaaaggtc
      481 tatttatcca atgttaccaa aacagacggt tcgattctga cttcttgaca gtccaaaaag
      541 taatcgaaag cggaaaatta ggggatattt tagaagtaga aatgcattat gattatttca
      601 gaccggaagt accagaaaat acgcatgaat tttctactgc gaacagctat ttatatggac
      661 atgcttgtca tacagtagat caagttcttt cttatttcgg cgaaccagat gatatcagct
      721 atgatgtacg tcaactttta gggaaaggac gaatgaatga ttacttcgat ctggattttt
      781 tctatggaaa ta
//
LOCUS       DASJON010000369          791 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.379, whole genome shotgun sequence.
ACCESSION   DASJON010000369 DASJON010000000
VERSION     DASJON010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.379"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="U3989_002958"
     CDS             7..624
                     /locus_tag="U3989_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716358.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          308 a           97 c          146 g          240 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt a
//
LOCUS       DASJON010000370          788 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.380, whole genome shotgun sequence.
ACCESSION   DASJON010000370 DASJON010000000
VERSION     DASJON010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.380"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..530
                     /locus_tag="U3989_002959"
     CDS             45..530
                     /locus_tag="U3989_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716359.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          243 a          139 c          132 g          274 t
ORIGIN      
        1 agtgatatac tgaataaaga taatctattt taggaggagc tatcatgcat aaagggattt
       61 acgcaggctt gttgggaact gtaatacttt tttctgcctg tggatataaa acaaagaatt
      121 ctgttaaaga atcttcagca gccatttcaa caactgaggt tggttcaact aaaagtactt
      181 ctactacttc cattactaat acttctactg gtaatgtaga ttcgcaacat agccaatcaa
      241 gtattcagtc gtttagcatt tcttcaacat ctgaaatgca agaaagcact gtaccttctc
      301 ttccattaga aaactctgac aaagcacttc tatttcttat tgcccaccgt tccgaattac
      361 aaagtgacga tattgttatt agcttttatc aaaaaattga tcgagattat ttgttcacag
      421 cttccagcaa acaaatccgt tcccaaggag gatctggaag cgttggtttt tatcgagtgt
      481 cacccgaagg atcgattacg atgacagatg caaatggaac accattttga ttagatgata
      541 ttatacggat ttacagtact agcaagtcat tattggtatc attttttctg agaaagttat
      601 acggagtata aataagcatt tgcattaacc taataattcg ttacagctat ttctcttatg
      661 aatatactca cttctctact tgctctcttg ataaattttt ggtatctact tcattttcgg
      721 tcaactgagt taatgaaatt tattgtcggg cggttttcta gattcaattt ttctatattt
      781 ggatagaa
//
LOCUS       DASJON010000371          781 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.381, whole genome shotgun sequence.
ACCESSION   DASJON010000371 DASJON010000000
VERSION     DASJON010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.381"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..255
                     /locus_tag="U3989_002960"
     CDS             52..255
                     /locus_tag="U3989_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716360.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            252..524
                     /locus_tag="U3989_002961"
     CDS             252..524
                     /locus_tag="U3989_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN1716361.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            525..710
                     /locus_tag="U3989_002962"
     CDS             525..710
                     /locus_tag="U3989_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716362.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
BASE COUNT          297 a          109 c          167 g          208 t
ORIGIN      
        1 tttagggggt cttacaaaaa cactaaaatt ttttcaaaaa gaggaggaac ggtggtgaaa
       61 gtcagagaac aattcaagat agtgagcgac gaaggtaatg tttacaaaat aaagatcgtt
      121 aatattaatg aatatcgaga agcagatcac aaatatttgg ttgacttata catgaacgat
      181 aaatatttgg gagaaggatt ttgtggagat tcactttttg atgatgagcg aattttgaag
      241 gaggaaacag aatgatccac gaactaaaaa tactaccaga atattttgaa gcagtgacga
      301 gcggacgcaa acaattcgag atccgcaaga atgatcgtga cttcaaagtt ggtgatcagt
      361 taatactacg tgaatataaa aaatacatta aacatgaatt acctggacta acgagaccaa
      421 catttagtta cactggtgac tcatacaaag cagagatcac ttatattact gattacatgc
      481 agaagtccgg atatgtagtg ttggggatta aaggggtgaa ttaaatgaaa gtatacatct
      541 tagctatcac agagggcaca tggatgttcc cagtcggttc cggaaaaatc tataaatcaa
      601 aaacagctgc ttataaagca tttgaaaaat acaaaaaaga aaacggtggc ggcactaacg
      661 caaagattct agtggctgat aactggcatg aagaaggtga acgtaattga tttacctgtt
      721 cattttcgtg gtactaacta tttttatgct agtaatctta ttcaaaaaaa gatgagtaaa
      781 g
//
LOCUS       DASJON010000372          770 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.382, whole genome shotgun sequence.
ACCESSION   DASJON010000372 DASJON010000000
VERSION     DASJON010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.382"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            254..733
                     /locus_tag="U3989_002963"
     CDS             254..733
                     /locus_tag="U3989_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716363.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT          289 a          116 c          154 g          211 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga
//
LOCUS       DASJON010000373          769 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.383, whole genome shotgun sequence.
ACCESSION   DASJON010000373 DASJON010000000
VERSION     DASJON010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.383"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U3989_002964"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U3989_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN1716364.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="U3989_002965"
     CDS             complement(481..675)
                     /locus_tag="U3989_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716365.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          229 a          161 c          133 g          246 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaag acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacgtt aaaaatgtt
//
LOCUS       DASJON010000374          751 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.384, whole genome shotgun sequence.
ACCESSION   DASJON010000374 DASJON010000000
VERSION     DASJON010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.384"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..>751
                     /locus_tag="U3989_002966"
     CDS             10..>751
                     /locus_tag="U3989_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1716366.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          254 a          131 c          149 g          217 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta g
//
LOCUS       DASJON010000375          725 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.385, whole genome shotgun sequence.
ACCESSION   DASJON010000375 DASJON010000000
VERSION     DASJON010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.385"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..>725
                     /locus_tag="U3989_002967"
     CDS             28..>725
                     /locus_tag="U3989_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN1716367.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQA
                     AYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQ
                     CYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYD
                     QLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DASJON010000376          718 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.386, whole genome shotgun sequence.
ACCESSION   DASJON010000376 DASJON010000000
VERSION     DASJON010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.386"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..316
                     /locus_tag="U3989_002968"
     CDS             <1..316
                     /locus_tag="U3989_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1716368.1"
                     /translation="IKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFI
                     QAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVP
                     VR"
     gene            330..>718
                     /locus_tag="U3989_002969"
     CDS             330..>718
                     /locus_tag="U3989_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716369.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCH"
BASE COUNT          211 a          140 c          130 g          237 t
ORIGIN      
        1 aataaaaaca aatgtggcga atgtctttgt cagtcccgaa gtggcatctt atgccttgca
       61 gttagtgtca gcaacaagaa gccatgctgc catccagctt ggcatcagtc cacgaggaag
      121 cctttccttt attcaggcag caaaagctta tgctttaatc catgggagaa attacgtcac
      181 gcccaaagat ttgcaggatt tagttcctta cgtgttttcc catcgcctga ttttttacga
      241 tcgttcattg aaggaagacg aaaaaaaaca aatcattcat actcttgttg cacaaattcc
      301 tgttcctgtg aggtagattg aggtagataa tggaaaaaat caaagattat ttcattaatt
      361 ttgtgttatt tctggtatat ttcatcattg tgctttttgc tcttgtattc aacaattctt
      421 taggctggtt cttgttggcc ttttttactc tttatgtatc atggagcttg atttcattaa
      481 gcagctttct aaagaaactt tcggttactt gtacattgca gccactcgtt tatcgcaaaa
      541 aaagtatgca ggttattttt tccatagcaa aaaagagaac tttttcttta cctctccctc
      601 gtttacaaat cggcttccca gcaatctttg cagaaaaaaa acaagagatt ttgattctta
      661 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatga
//
LOCUS       DASJON010000377          712 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.387, whole genome shotgun sequence.
ACCESSION   DASJON010000377 DASJON010000000
VERSION     DASJON010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.387"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..569)
                     /locus_tag="U3989_002970"
     CDS             complement(<1..569)
                     /locus_tag="U3989_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1716370.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKV"
BASE COUNT          193 a          142 c           99 g          278 t
ORIGIN      
        1 tgtaccttca aaccaagtac atgattaaga aattggcgtt cttttgttaa gttagtaaca
       61 tttaaatgga ttttttcaaa ataagaacct gttgatagtt tactgaattt ttcttcagat
      121 tttcttaata atgcttcttg atccattgtt tctggaattt ctctttggtc gttcttattc
      181 tcttcatggt aaatttccaa ttcattctct tctgggtcta cgagcaaaat ccccatacgt
      241 ccatgatctt ctaaagcttc tttgattgga taattcattt tacggatacg acaaaggata
      301 tctgccattt cttctgtgct agggataatc aatgaaaaac ggttcagctt tttgatcacc
      361 cccatgtgat cattcgctct aggactttct tccagcagca acatttcact tccagcttct
      421 tctgttccta aaatggctaa ttcgttttct tctcttttca aatcaaagcc aacaacgttc
      481 ttgtaaaaat caatcatttt atctcggtct ttcacccgaa tggctacact ttttaaatag
      541 gtggcagccc ccaattgaaa atctttcata tttcttgctc ccctctacgt caagctttat
      601 tttaacgatg gattcttctt ctttcaagca ataactttga ttttgttaaa aaaaatgtga
      661 gaaaattcgc attttgcttg ttttttgtaa attaatgagt aaaaactctt at
//
LOCUS       DASJON010000378          676 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.388, whole genome shotgun sequence.
ACCESSION   DASJON010000378 DASJON010000000
VERSION     DASJON010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.388"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..105)
                     /locus_tag="U3989_002971"
     CDS             complement(<1..105)
                     /locus_tag="U3989_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1716371.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEE"
     gene            289..>676
                     /locus_tag="U3989_002972"
     CDS             289..>676
                     /locus_tag="U3989_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1716372.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIR"
BASE COUNT          215 a          109 c          132 g          220 t
ORIGIN      
        1 ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat agataatttg
       61 tttacgaatc gcttgataag cttgtgattg caagctgctt ttcatcttta ttcctccaaa
      121 atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg tatgtcacat
      181 taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt ttattgcata
      241 aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat gattgattta
      301 aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat acctcatttt
      361 gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca tttcggtggc
      421 ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa
      481 ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga aagggcttac
      541 caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa
      601 cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc tgaacacttt
      661 gataagatga ttcgac
//
LOCUS       DASJON010000379          670 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.389, whole genome shotgun sequence.
ACCESSION   DASJON010000379 DASJON010000000
VERSION     DASJON010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.389"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..417
                     /locus_tag="U3989_002973"
     CDS             154..417
                     /locus_tag="U3989_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1716373.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="U3989_002974"
     CDS             417..>670
                     /locus_tag="U3989_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1716374.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DASJON010000380          656 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.390, whole genome shotgun sequence.
ACCESSION   DASJON010000380 DASJON010000000
VERSION     DASJON010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.390"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..167)
                     /locus_tag="U3989_002975"
     CDS             complement(<1..167)
                     /locus_tag="U3989_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1716375.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVM"
     gene            complement(154..582)
                     /locus_tag="U3989_002976"
     CDS             complement(154..582)
                     /locus_tag="U3989_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1716376.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT          192 a          142 c           95 g          227 t
ORIGIN      
        1 tacataacca ttcctttttc ttcttcataa actggtttca tggctaaccg tgaaatataa
       61 ttttgatatt ttgacagaac gatggacatg gcaattgaat caccagaagc tgcggctttg
      121 attgtcgtta gtggtatgaa tggtatatat cgtttatttt gcttcatatt tgacacccaa
      181 atactttcct agctcttcca aagccatttg tcgttggtac caaacactgc cgagactttt
      241 cccaataata tcgccaattt cacgatcatt aagaccgatg aagaaggaca taaaaatgat
      301 ttcttgtctt tgactaggta atgctgcaat cgcatttgcc aactcctcat tggaaataaa
      361 gattttcata ccaaggacaa taaaagtacc actactattc aaatttggtt ccaaataata
      421 gaactctgta cctaaccctt tttgttccaa tatttccatt gatatttgcc gttggcgaaa
      481 tctagcgtac ttcttttgaa tatttaacgc ctcattgcag actactttct tacaaaaact
      541 gtcaaacgta tgttgaatgt gggtttcctc tgagtcgccc ataatattct ccccctcaaa
      601 aaattcattc aactcgtttt acttctatcc ctttccatta ttactacaat ctagga
//
LOCUS       DASJON010000381          656 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.391, whole genome shotgun sequence.
ACCESSION   DASJON010000381 DASJON010000000
VERSION     DASJON010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.391"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..514
                     /locus_tag="U3989_002977"
     CDS             <1..514
                     /locus_tag="U3989_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEN1716377.1"
                     /translation="FETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETPFFWRNVQ
                     IHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYYHLEQPNY
                     LSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLYQEKKFGT
                     DPLKKLVTIK"
BASE COUNT          210 a          131 c          125 g          190 t
ORIGIN      
        1 ttttgaaact gccaatcatc agccttatag tgcttgttct cttgcgctag tcatggtaaa
       61 aaatagccag atcgttgatg aattctatac attgatacag cctgagactc cttttttctg
      121 gcgaaatgtc caaattcacg gaatccatca agaagacgta cgcaatgcgc cgaaattccc
      181 agaagtctgg caaactatcc aaccatattt tcaaaaaaat cgtttagtcg ttgcacataa
      241 tgctgctttt gatacaaagg tcttagcggg atgcttagat tattatcatc ttgaacagcc
      301 taattatctt tctttatgta cagtgaaaag tagccgtcgc ctgtttcctg aatttccaaa
      361 tcatcgattg aacactgtgt gcgaaaattt gaacatccaa ttagatcatc accatgatgc
      421 attggaagat agtcgtgcat gtgcggaaat cctgctttat caagaaaaaa aattcgggac
      481 agatccattg aaaaaacttg tgactataaa gtaaaagaat acttatgaaa cacacaaaga
      541 ggtcgtgagc ctgccgtttc actccgagtc acaaggagca atttcaaaaa atagcttctc
      601 atgtttatta aatattgtga ctggtgaccg aggagctggc aggagaacac cgttta
//
LOCUS       DASJON010000382          651 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.392, whole genome shotgun sequence.
ACCESSION   DASJON010000382 DASJON010000000
VERSION     DASJON010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.392"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..428
                     /locus_tag="U3989_002978"
     CDS             <1..428
                     /locus_tag="U3989_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716378.1"
                     /translation="ANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQYQFKDKHLT
                     VLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQKNES
                     TGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT          233 a          117 c          133 g          168 t
ORIGIN      
        1 ctgccaatta cgaaaaaggc gaattggaca tttatttgcc aaaaggaaaa gaagaaaatc
       61 aatttattaa tatcgatgac gtacttagtg cgatttctct cagacaatat caatttaaag
      121 acaagcattt gaccgttttg cttttgactc atgatggcga caaagtagcc cgtttgaaaa
      181 atggaaaagt gatcaccgct tattctgaaa aagtagacaa aaaagtggtc gccgatcgtt
      241 tagcggaatg gaaaaagaca aatgaacaag tcaagaaaac aactgtctct tctcaaaaaa
      301 acgagtcgac aggaacaagt ccttcagatt ctggcagtgg acaaacgata acgggaagtg
      361 aagaaaatca agcctcaatt attacgaatg agtcatcaca aggacaatat caatcctatg
      421 gacaatgaaa aacagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac
      481 ggcaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
      541 aacaccgtgt atcaagaggt cttgagcctg ccgtttaact ctgagccaca aggagcaatt
      601 tcaaaaaata gctccttata tttttcgttt ggttgggctt attaccgcag a
//
LOCUS       DASJON010000383          648 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.393, whole genome shotgun sequence.
ACCESSION   DASJON010000383 DASJON010000000
VERSION     DASJON010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.393"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..630
                     /locus_tag="U3989_002979"
     CDS             <1..630
                     /locus_tag="U3989_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1716379.1"
                     /translation="GETIAFVGPSGSGKSTLLNLLIGFIQPTSGKIFLDDQPLDELDM
                     RSVRNYLAVVPQTTLLFSASIKENITYGLKNVSKERLDEVIEAAQLSSLIDQLPDGLD
                     TLVGEHGNKLSGGQKQRISIARALIRQPKIILLDEATSALDNQSEKKIQQALNYLTET
                     PTTFIVAHRLSTIKEADKIVVIDEGKIVEIGTYEKLLAQKGYFYRLKNE"
BASE COUNT          208 a          132 c          132 g          176 t
ORIGIN      
        1 ggagaaacaa ttgcttttgt tgggccatct ggctctggaa aatctacact ccttaactta
       61 ttgattggat ttatccagcc gacttcagga aaaatttttc tagatgatca gcctttggat
      121 gaactggata tgcgaagtgt gcgaaattat ttagccgttg ttcctcaaac cactttgttg
      181 ttctccgctt cgatcaaaga gaacatcact tatggcttga aaaacgtatc aaaagaacgt
      241 ttagatgaag tcatcgaagc tgcgcaactt tcttcactga ttgaccagct tcctgatggt
      301 ctggatacct tagtaggcga acatggcaac aagctgtctg gtggccaaaa gcaacgtatc
      361 tccattgcac gagcgttgat acgtcagcca aaaatcattt tgctagatga agcaacctca
      421 gctttagata accagtcaga gaaaaaaatc cagcaagccc tgaattacct gacagaaaca
      481 cccactactt ttattgtggc tcatcggctt tcaacgatca aagaggcaga taaaatcgtt
      541 gtgatcgatg aaggaaaaat cgttgaaata ggtacttacg aaaaactgtt agctcaaaaa
      601 ggctatttct accgcttgaa aaatgaatga caaaaaatag gagctatg
//
LOCUS       DASJON010000384          637 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.394, whole genome shotgun sequence.
ACCESSION   DASJON010000384 DASJON010000000
VERSION     DASJON010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.394"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            369..637
                     /locus_tag="U3989_002980"
                     /pseudo
     CDS             369..637
                     /locus_tag="U3989_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          220 a          107 c          115 g          195 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaact
//
LOCUS       DASJON010000385          631 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.395, whole genome shotgun sequence.
ACCESSION   DASJON010000385 DASJON010000000
VERSION     DASJON010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.395"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(224..424)
                     /locus_tag="U3989_002981"
     CDS             complement(224..424)
                     /locus_tag="U3989_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1716380.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          236 a           95 c          103 g          197 t
ORIGIN      
        1 aacaaacgtt ctttttgtaa aggtggaatt tattatacta aataaatagg tgaacaagta
       61 cttgtgccaa attatgtgcc aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat
      121 cctattatga taacatttct tataataaca aacacaacag aagacgtgta atagttagtc
      181 aggaacgtac aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag
      241 acattgtagt ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc
      301 cttgataaag taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag
      361 aaaataggga aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt
      421 tcatacttca atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac
      481 gaaagatata tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg
      541 gaatgaactg gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca
      601 actttccttt tcaaagtacc atataaaaaa a
//
LOCUS       DASJON010000386          628 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.396, whole genome shotgun sequence.
ACCESSION   DASJON010000386 DASJON010000000
VERSION     DASJON010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.396"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>628
                     /locus_tag="U3989_002982"
     CDS             <1..>628
                     /locus_tag="U3989_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1716381.1"
                     /translation="YFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYA
                     LKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAW
                     VPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVV
                     PITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIP"
BASE COUNT          199 a           78 c          119 g          232 t
ORIGIN      
        1 tatattttga tgataaatat gttggcagag tagtcggcgg aacactgtca tttgtgttga
       61 taggtttagt aatttattta agaataatga taaagggtgc tcattttttt gataaaaaac
      121 agtggagata cgctttaaaa atctctttgc cgctaatccc acattcctta gcaaatatag
      181 ttttagcaca attcgataga attatgatta attcttattt aggtcctgct gcggggggaa
      241 tatattccta cattagtaac ataggtatta ttctaagcgt aatatggatg tctactaata
      301 acgcctgggt tccatggttc tatggtgaga tgagtaaaaa taatgaaaaa aaagtgaaga
      361 aagttagtaa ctattattta gtagtcttta ctttgattac gatgattgta atggtagttt
      421 cgattgattt agcaaaattt atggctccga aagactatta ttctgggtta tctttagtgg
      481 ttccgataac cttaggctat ttctttcagt ttttatatag tttaccagtt aatgctgaat
      541 tttatgagaa aaaaacatcg tttattgcga ttggtacagt agcaagtgct atagttaata
      601 tactattaaa catgattttc atacctag
//
LOCUS       DASJON010000387          616 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.397, whole genome shotgun sequence.
ACCESSION   DASJON010000387 DASJON010000000
VERSION     DASJON010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.397"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..>616
                     /locus_tag="U3989_002983"
     CDS             51..>616
                     /locus_tag="U3989_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEN1716382.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          223 a          103 c          120 g          170 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccac
//
LOCUS       DASJON010000388          612 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.398, whole genome shotgun sequence.
ACCESSION   DASJON010000388 DASJON010000000
VERSION     DASJON010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.398"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          188 a          132 c           90 g          202 t
ORIGIN      
        1 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
       61 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
      121 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
      181 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
      241 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
      301 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
      361 caacctcttg gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta
      421 aaatatgaga agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt
      481 tacaacctct gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc
      541 aaaatatgag gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt
      601 tacaacctcc tg
//
LOCUS       DASJON010000389          595 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.399, whole genome shotgun sequence.
ACCESSION   DASJON010000389 DASJON010000000
VERSION     DASJON010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.399"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>595
                     /locus_tag="U3989_002984"
     CDS             <1..>595
                     /locus_tag="U3989_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN1716383.1"
                     /translation="DYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGR
                     KERSVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYP
                     DFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRF
                     PKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          203 a          107 c          111 g          174 t
ORIGIN      
        1 ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat
       61 tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg
      121 tcattatcgg gcgaaaagaa aggtctgttt catgcggtat gagaacactt tatcgtttat
      181 tttctaaagg aatatttgat attgacacac taccgatgaa aggtaaaaga aaacccaatg
      241 gccatcagga aaaacgggga aaacaacagt atcagcgctc aatccatgat cgacctgata
      301 attatcctga tttcaattct gagtttggtc accttgaagg tgatacgatc gttggcattc
      361 atcataaaag tgccgtcatt actttagttg aaagattatc taaagtcatt atcacgatta
      421 aacccaacgg ccgtaaggca ttagatattg aaactgccct taatcaatgg ttttctcgct
      481 tccctaaaaa cttctttaaa tctattacgt ttgactgtgg aaaagaattt tctaactgga
      541 aagccattag taaccaacat gatattgata tatattttgc ggaccctgga acacc
//
LOCUS       DASJON010000390          593 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.400, whole genome shotgun sequence.
ACCESSION   DASJON010000390 DASJON010000000
VERSION     DASJON010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.400"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..580
                     /locus_tag="U3989_002985"
     CDS             <1..580
                     /locus_tag="U3989_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1716384.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DASJON010000391          532 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.401, whole genome shotgun sequence.
ACCESSION   DASJON010000391 DASJON010000000
VERSION     DASJON010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.401"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..508
                     /locus_tag="U3989_002986"
     CDS             <1..508
                     /locus_tag="U3989_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HEN1716385.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          160 a           96 c          114 g          162 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aa
//
LOCUS       DASJON010000392          532 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.402, whole genome shotgun sequence.
ACCESSION   DASJON010000392 DASJON010000000
VERSION     DASJON010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.402"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(35..319)
                     /locus_tag="U3989_002987"
     CDS             complement(35..319)
                     /locus_tag="U3989_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN1716386.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT          147 a          123 c           83 g          179 t
ORIGIN      
        1 agaggttgtg acacgtcttt tgtcacaacc tcttttattt ttgcttcact gtaactgcta
       61 caatatcacc gatttctttt ccgatgtttt tacgaatatc ttttcgtagt ccaaggatgt
      121 agcagactga accatctgga tttttcacgc ccatattcac gatactgcct tcatacggca
      181 cattgtcaaa agttgcttgg actttaactc ttccagcacc gaattctttt cttatatcat
      241 acggaaaaat aatatatgcc ccgcccttgc cgacttcgga agcatagatt tttgattcaa
      301 aagaataaat ttccctcatt ttatttctcc tattttttac atctagtata gcatagcaaa
      361 aaaaagaagg aactgtgaca aaagtcttgt cgcagttcct tttttcgaat aaacggtgtt
      421 ctcctgccaa ctcctcggtc acaagtcaca atagtcaaca aacatgagaa gctgtttttt
      481 gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctcttc ct
//
LOCUS       DASJON010000393          528 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.403, whole genome shotgun sequence.
ACCESSION   DASJON010000393 DASJON010000000
VERSION     DASJON010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.403"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..90)
                     /locus_tag="U3989_002988"
     tRNA            complement(5..90)
                     /locus_tag="U3989_002988"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="U3989_002989"
     tRNA            complement(107..178)
                     /locus_tag="U3989_002989"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="U3989_002990"
     tRNA            complement(190..262)
                     /locus_tag="U3989_002990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="U3989_002991"
     tRNA            complement(280..361)
                     /locus_tag="U3989_002991"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="U3989_002992"
     tRNA            complement(369..441)
                     /locus_tag="U3989_002992"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="U3989_002993"
     tRNA            complement(445..517)
                     /locus_tag="U3989_002993"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DASJON010000394          524 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.404, whole genome shotgun sequence.
ACCESSION   DASJON010000394 DASJON010000000
VERSION     DASJON010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.404"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>524
                     /locus_tag="U3989_002994"
     CDS             <1..>524
                     /locus_tag="U3989_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN1716387.1"
                     /translation="FRKSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGL
                     DFREHRAYRPGDSFNRIDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSHSPKFEPLL
                     DLYFSLWHLRKEKQPELLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQI
                     VLFVPENSPEVSEA"
BASE COUNT          156 a          106 c          105 g          157 t
ORIGIN      
        1 ttcagaaaaa gtgcaaaaag agagatctct tttcctgtga ctgttctgcc tgctttttgt
       61 aagattcaag cagaaaaatt acagagagtt ctggaaaaaa aacagcagct gaacgcgtac
      121 caaagaggtc ttgattttcg ggaacatcgc gcttatcgtc caggtgattc tttcaaccga
      181 atcgattgga aattgtctgc tcatactcaa gagtggttat accgtgaata cgattatcaa
      241 gaagaagaat gttctcctct tttttgcttt tggggttcgc acagtccaaa atttgaacct
      301 ctgctagact tgtatttttc tttatggcat ttgagaaaag aaaagcagcc ggaacttctg
      361 atattaggca accgcgcctt tcatggcaaa gaccccggtc atacctattt tgcttctttg
      421 gaagctggcg atgagaaagc ttttttcgca cacctaaaaa aatatgcaaa aaaacaaatc
      481 gtcctctttg ttcctgagaa ttctcctgaa gtctcagaag ctat
//
LOCUS       DASJON010000395          514 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.405, whole genome shotgun sequence.
ACCESSION   DASJON010000395 DASJON010000000
VERSION     DASJON010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.405"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="U3989_002995"
     CDS             complement(<1..132)
                     /locus_tag="U3989_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1716388.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DASJON010000396          513 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.406, whole genome shotgun sequence.
ACCESSION   DASJON010000396 DASJON010000000
VERSION     DASJON010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.406"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          175 a           98 c           59 g          181 t
ORIGIN      
        1 aacacagtta tttttaaaaa taagaatgct aaatcactct acaataacgg cgaaagcaca
       61 aaaaataaaa aaattattac gttattaaaa aaacagatga aagatcaggt tttattagat
      121 ctcattcttt catcacttga agggatttaa tgaactactt tttcctcttt gtcgttttta
      181 tatattcgta ttccttcaaa cagtaggata gcctctttca atgcttgata tttgaactgt
      241 ccgttactta aaaatttaga tgtgccgccc tcttcctaga acatattctt ttttactttt
      301 tcattaattg acagaccaat cgaacgaaac tgtaaatttc gataggataa tgattggtca
      361 aactcattca ttttcaagac atctcgattc atattcaaga taggaatctt tttatcaaca
      421 tcactttatt cgtattttcc tctaccataa aagcttcacg ctccacaaaa ttgttttata
      481 cactactatt cagcccattc aacgaccaat gaa
//
LOCUS       DASJON010000397          496 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.407, whole genome shotgun sequence.
ACCESSION   DASJON010000397 DASJON010000000
VERSION     DASJON010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.407"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..327
                     /locus_tag="U3989_002996"
     CDS             <1..327
                     /locus_tag="U3989_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1716389.1"
                     /translation="KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQ
                     REGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRK
                     LSEVNN"
BASE COUNT          199 a           66 c           90 g          141 t
ORIGIN      
        1 aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga agataatcca
       61 tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg agatcttatt
      121 cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt taaaggtcga
      181 ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct atataaagaa
      241 ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc ttcattatac
      301 aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg caatattttt
      361 aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga aaagcccttt
      421 gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta aatgcttata
      481 aattcggccc tataat
//
LOCUS       DASJON010000398          496 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.408, whole genome shotgun sequence.
ACCESSION   DASJON010000398 DASJON010000000
VERSION     DASJON010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.408"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..452
                     /locus_tag="U3989_002997"
     CDS             <1..452
                     /locus_tag="U3989_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEN1716390.1"
                     /translation="LVSAENARFVREQLEEQIMIECCSKLDTQTRIVLETILEEQEKA
                     VKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRWLRVTIQGLPWETI
                     MEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYFQQ"
BASE COUNT          164 a           90 c           99 g          143 t
ORIGIN      
        1 atcttgtttc tgcagaaaat gctcgctttg tccgagaaca gttggaagag caaatcatga
       61 ttgaatgttg ttccaaattg gacacacaaa cacgaatagt gctagaaacg attttagaag
      121 aacaagaaaa agcagtcaaa ataaatgacg aacgcgcttt tttccgtgca gataatttat
      181 ttcatgaagc ttgtttcgaa attgcaggaa gaaaagaagt ttggaactgg ttagatgacc
      241 ataataccca tttagaacgt ttccgctggt tacgagtgac cattcagggg cttccatggg
      301 aaacgatcat ggaacaacat tatgatttac tcaaggcttt agttgataaa aaaccagaag
      361 aagctcgatt tcaaacggga ttgcacttac acatgatgct gaaagaacag gaaactgtta
      421 ctcaacgtta tcccgagtat ttccagcagt agcattttct tgcgacctct tctattttta
      481 taattaacct tcgttt
//
LOCUS       DASJON010000399          493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.409, whole genome shotgun sequence.
ACCESSION   DASJON010000399 DASJON010000000
VERSION     DASJON010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.409"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(102..473)
                     /locus_tag="U3989_002998"
     CDS             complement(102..473)
                     /locus_tag="U3989_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716391.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          157 a           86 c           61 g          189 t
ORIGIN      
        1 agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga aagatacaaa
       61 gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag tttgtttatt
      121 attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt ttcattagga
      181 atatagtcag cacattttct tttctctaac attctatacc cactattatg tattcctaag
      241 gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct tccttttccg
      301 ctacttccat tataaataat atcataattt tctttcaaat actcatcaga ttttttaaaa
      361 gcactgtagt atgctcttga aacagcagtt cttctataag attctttaga attatattta
      421 tcatctgatt caatttgttt ggaaatagat aaatattcat taaagtcaaa cattttattc
      481 ccccaaagtt gct
//
LOCUS       DASJON010000400          493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.410, whole genome shotgun sequence.
ACCESSION   DASJON010000400 DASJON010000000
VERSION     DASJON010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.410"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>493)
                     /locus_tag="U3989_002999"
     CDS             complement(<1..>493)
                     /locus_tag="U3989_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716392.1"
                     /translation="NKLSKTTIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFY
                     LFLMGYGLKEWNWKEKVRYKLPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKP
                     YSLFAMYYTFSSFLFLEMLSDKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKR
                     EFPN"
BASE COUNT          194 a           76 c           70 g          153 t
ORIGIN      
        1 gtttggaaat tcacgtttca taaatgtaga tacgcgataa gaaactaaag ggaaattaca
       61 tataacttga gtgacaatca aaaatacagc aagaaaattt cctcttcctt ttatacctaa
      121 tttatcagat agcatttcta aaaataaaaa cgaagagaaa gtataataca ttgcgaagag
      181 agaatatggt ttagaaaacc tcagcgctgt atctgtattt cctctgatga tgagtgaaat
      241 ttgtgtcatc aaaataatca aaccgaataa aacaatcaag ctgataaata cttgaggaag
      301 cttatagcgg accttttctt tccagttcca ttctttcaaa ccataaccca ttaaaaataa
      361 ataaaaaatc cataaaatac tctgtccatt ctgtaatgca aacgggtctt tattaaatag
      421 agtagagcag ccaactaatc caattgttaa aaataatagt agatatttaa ttgttgtttt
      481 cgataattta ttt
//
LOCUS       DASJON010000401          473 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.411, whole genome shotgun sequence.
ACCESSION   DASJON010000401 DASJON010000000
VERSION     DASJON010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.411"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>473
                     /locus_tag="U3989_003000"
     CDS             <1..>473
                     /locus_tag="U3989_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1716393.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DASJON010000402          469 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.412, whole genome shotgun sequence.
ACCESSION   DASJON010000402 DASJON010000000
VERSION     DASJON010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.412"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            93..>469
                     /locus_tag="U3989_003001"
     CDS             93..>469
                     /locus_tag="U3989_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1716394.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSV"
BASE COUNT          108 a           68 c          108 g          185 t
ORIGIN      
        1 tgttggtttt tttgtgagta tattttccta ttttttcaat aatgtatgtt atgctaagtt
       61 cgggctatat tttatacaaa ggaatgatga gaatggaatt tattggtatt ttatgcctga
      121 tattgatttc aacaacgatt ggctcacata tctcgcggag attaggtatc ccagcagtga
      181 ttggacaatt acttgttggt gttttgctag gccaagctgg attgaattgg gtcaaacctg
      241 atattctagt ccatgacttt tcggagattg gtgtgatctt attaatgttt ttagctggtc
      301 tggaaagtga tttatctctt ttgaaaaaat atttccgacc tggtatgttc gttgctttgc
      361 ttgggatcat cttcccggtt gtttttggct ggttgacagg caaagcattc caagtttcgg
      421 gaaatgaagc gattttcttt gggattattc ttgcggcaac ttctgtcag
//
LOCUS       DASJON010000403          468 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.413, whole genome shotgun sequence.
ACCESSION   DASJON010000403 DASJON010000000
VERSION     DASJON010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.413"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>468
                     /locus_tag="U3989_003002"
     CDS             <1..>468
                     /locus_tag="U3989_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN1716395.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DASJON010000404          464 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.414, whole genome shotgun sequence.
ACCESSION   DASJON010000404 DASJON010000000
VERSION     DASJON010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.414"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..449
                     /locus_tag="U3989_003003"
     CDS             <1..449
                     /locus_tag="U3989_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEN1716396.1"
                     /translation="ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVF
                     YPIEKQLGMIEEDPNTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYD
                     AYAKDPVVTRYIRKLFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT          171 a           73 c           90 g          130 t
ORIGIN      
        1 atgagacata tgaaatggtt tttacagcag caaatccagc attggtacat cttattcaat
       61 tagaaaaatt gatgaaaaag tcaagcaaat ggtgcgtgat aatcagagta gtggaatcaa
      121 tcgatgatgt tttttatcct atcgaaaagc aactaggaat gatagaagaa gatcctaata
      181 catcgccagt tgtaatggat caatttgaaa catacttaat tgatgaagga ttcaaagtga
      241 agaaaaaaga ttttacttat tttacacagg aatcaattac tgaagaattg atgaagctat
      301 actatgacgc gtacgcaaaa gatcctgttg tgaccaggta cattcgcaaa ctattcagtg
      361 aaaaacgaga aatccttagt acgactaagc tgacctatag actgctcctt tggtccgcag
      421 ataaaaaaag aacctttcac gactcgtgaa gggttctttt tatg
//
LOCUS       DASJON010000405          458 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.415, whole genome shotgun sequence.
ACCESSION   DASJON010000405 DASJON010000000
VERSION     DASJON010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.415"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            35..205
                     /locus_tag="U3989_003004"
     CDS             35..205
                     /locus_tag="U3989_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716397.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT          176 a           57 c           69 g          156 t
ORIGIN      
        1 aatattggat acagattgcg aaaggagaga gcgaatgatt acaataatta aacctcagga
       61 aaaatcccct gttttgaaat ttaatgagag caatctagcc cataataaaa tgagtataac
      121 tgatacagta tttgatgtaa tggttgagca acaaaaaaaa attaataata acaatagaca
      181 aagaaaagaa attaaccaat attaatgttt tttctgttgc gttttttgca gtcataattt
      241 aaaacctcgc tcaatcttgg gcgaggtctt tttttatttt tctgaaaagt taaagtaaat
      301 aatagcataa aataatatat tttacaaaga ataagtacaa tctagttttt tgctattaaa
      361 tgtgtaataa taaatatgcc atcacaacat gaagaatgaa atccattatt atctagtcta
      421 gtccattctt tttgtttgca gtatttgtga tggctttc
//
LOCUS       DASJON010000406          438 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.416, whole genome shotgun sequence.
ACCESSION   DASJON010000406 DASJON010000000
VERSION     DASJON010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.416"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            71..>438
                     /locus_tag="U3989_003005"
     CDS             71..>438
                     /locus_tag="U3989_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716398.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          167 a           79 c           92 g          100 t
ORIGIN      
        1 tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa aaattgggga
       61 ggtatagaga atgacagata tcgtaaaaat aaaacaaagc aatgtacagg tttatccaca
      121 aactcattgg aatgctatag aaggcaaacc aacaacggtg aagggagata aaggcgatcc
      181 tggccaagca gcaacaatta ccataggaac cgtttctagt ggttcaaccg cttctgttac
      241 gaatgtagga acatcatctg cagctagatt taattttgtt ttaccaaagg gagataaagg
      301 tgatcctgga ataaatgcca caactacagc tgtagctaca actacagcaa atgggttgat
      361 gtcgtccaca gataaaacta agttggatgg aattgcagct ggagcacaga aaaatccagg
      421 taatgctaca actacgac
//
LOCUS       DASJON010000407          437 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.417, whole genome shotgun sequence.
ACCESSION   DASJON010000407 DASJON010000000
VERSION     DASJON010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.417"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..437
                     /locus_tag="U3989_003006"
                     /pseudo
     CDS             1..437
                     /locus_tag="U3989_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DASJON010000408          421 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.418, whole genome shotgun sequence.
ACCESSION   DASJON010000408 DASJON010000000
VERSION     DASJON010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.418"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..355)
                     /locus_tag="U3989_003007"
     CDS             complement(170..355)
                     /locus_tag="U3989_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HEN1716399.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DASJON010000409          420 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.419, whole genome shotgun sequence.
ACCESSION   DASJON010000409 DASJON010000000
VERSION     DASJON010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.419"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..403
                     /locus_tag="U3989_003008"
     CDS             74..403
                     /locus_tag="U3989_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1716400.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DASJON010000410          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.420, whole genome shotgun sequence.
ACCESSION   DASJON010000410 DASJON010000000
VERSION     DASJON010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.420"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..390
                     /locus_tag="U3989_003009"
     CDS             <1..390
                     /locus_tag="U3989_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1716401.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          115 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatt
//
LOCUS       DASJON010000411          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.421, whole genome shotgun sequence.
ACCESSION   DASJON010000411 DASJON010000000
VERSION     DASJON010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.421"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..339)
                     /locus_tag="U3989_003010"
     CDS             complement(<1..339)
                     /locus_tag="U3989_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1716402.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DASJON010000412          403 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.422, whole genome shotgun sequence.
ACCESSION   DASJON010000412 DASJON010000000
VERSION     DASJON010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.422"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..294)
                     /locus_tag="U3989_003011"
     CDS             complement(79..294)
                     /locus_tag="U3989_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN1716403.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
BASE COUNT          161 a           72 c           50 g          120 t
ORIGIN      
        1 gatggtttaa aatgaaaaaa ccttcccaat aaagggaagg ttcatgaaga taataacttt
       61 tttaataatt atagctttct aatatctttc atcaactttt ctagtaatca ttgggaaaaa
      121 tttcaagcag tagatcgcac aaatacctac gacgatataa atgatcttcg caaaaatagt
      181 tgttgaacca ccagcaatca tagctactaa atcaagttcg aacaagccta ccaacagcca
      241 gttcaaccct cccacaatca ataaagttaa tgcaatagca tctaaagttt tcattagaat
      301 tctcctttct taaatgataa aacaattata tatcaaaaag agaaacattg cttatataac
      361 gctcaggaat tttagcaatc tatgcaaaaa gatgaaaatt ttt
//
LOCUS       DASJON010000413          396 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.423, whole genome shotgun sequence.
ACCESSION   DASJON010000413 DASJON010000000
VERSION     DASJON010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.423"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..256)
                     /locus_tag="U3989_003012"
     CDS             complement(32..256)
                     /locus_tag="U3989_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1716404.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          103 a           89 c           58 g          146 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt attcattttc ttctctccct aaataaagtt ttaatttgtt gcgtgtgttc
      301 caccaatttt tctgtatgtg tatctaatct ttcatcgtgt ttctttagtt cttcatgaat
      361 catcaatcga tctgatttgc tcgattctaa atcttt
//
LOCUS       DASJON010000414          394 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.424, whole genome shotgun sequence.
ACCESSION   DASJON010000414 DASJON010000000
VERSION     DASJON010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.424"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..253
                     /locus_tag="U3989_003013"
     CDS             <1..253
                     /locus_tag="U3989_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1716405.1"
                     /translation="KNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERD
                     IDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          153 a           39 c           69 g          133 t
ORIGIN      
        1 taaaaatgtt attggaggac cagatttacc acttgaattg aaaaatattg aagaaactac
       61 tttacaaact tcgaaattag caaatattca tattattgat ccttccatga acaaatggtt
      121 tgatgaaagg gatatagatc aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa
      181 atcggattac tctgataaac tattaaatag aattgataaa gaaattttga aagttggaaa
      241 tatagatgaa tagaatagta cctgttttag agaagagttt attaagtcga gatgcgtttc
      301 gagtaataaa tttaatcatt tgtctgcttt attttatgcc tagtactttt aatgtaacta
      361 atatcccgat gaaaatagct tttgcttggg gtgg
//
LOCUS       DASJON010000415          388 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.425, whole genome shotgun sequence.
ACCESSION   DASJON010000415 DASJON010000000
VERSION     DASJON010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.425"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          131 a           65 c           66 g          126 t
ORIGIN      
        1 actgtaacat acttttgaaa aaagcaaata aatttaacaa aaaacataga attttttatg
       61 taatatttgt tgagaaaagc tagtttttga actcattcct tgtgatttgg agttagatgg
      121 ggatcatgac ttctcggtat taagtcaaat ttatgcaaag ttgtgaggaa ccattttcaa
      181 aaaaatttct tacgactcga aagtaggcga ctttttcaca acttctccac taaacggtgt
      241 tcaatcagct gaccctcggt attagctcaa aaaatagcaa aatatgagga actattttgc
      301 catttgtttc tctaatactc aggtcaggac gctgatttca caacttctca ataaacggtg
      361 ttcaaaagct tgcatctgcg gtaataag
//
LOCUS       DASJON010000416          384 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.426, whole genome shotgun sequence.
ACCESSION   DASJON010000416 DASJON010000000
VERSION     DASJON010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.426"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..118)
                     /locus_tag="U3989_003014"
     CDS             complement(<1..118)
                     /locus_tag="U3989_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1716406.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNL"
     gene            complement(105..347)
                     /gene="yaaA"
                     /locus_tag="U3989_003015"
     CDS             complement(105..347)
                     /gene="yaaA"
                     /locus_tag="U3989_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN1716407.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
BASE COUNT           98 a           89 c           56 g          141 t
ORIGIN      
        1 aaagattcgt ttttccttgc gcattttctc ctaaaaagat gacgaggttc ttggaaaatt
       61 cgatgttcag ctcttcgtaa tttcgataat tttttaaata cagcttattc agcctcatta
      121 tcgtcgtttc ctttttttac cataaaaaaa gttcctactt ctggaacttc aaccatcatg
      181 ccgggataaa gtttgcgtcc acgacgattt tctggttcgc catcgactaa cacagtgttt
      241 tctgccaaat accattttgc ttgtccgcca ctaccgataa ctgtgacttc tttcaacatt
      301 tggcccaacg tcatgaattc cgtatttaga atgacttgtt gtttcaatgc ccatccctca
      361 ttttcattat taaaagtata ctct
//
LOCUS       DASJON010000417          369 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.427, whole genome shotgun sequence.
ACCESSION   DASJON010000417 DASJON010000000
VERSION     DASJON010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.427"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..369
                     /locus_tag="U3989_003016"
                     /pseudo
     CDS             1..369
                     /locus_tag="U3989_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          145 a           74 c           66 g           84 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaaa attctaaata tttctcgtca aacctactat tatcaagcga aaccgatcga
      361 aaatgagtc
//
LOCUS       DASJON010000418          362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.428, whole genome shotgun sequence.
ACCESSION   DASJON010000418 DASJON010000000
VERSION     DASJON010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.428"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>362)
                     /locus_tag="U3989_003017"
     rRNA            complement(<1..>362)
                     /locus_tag="U3989_003017"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DASJON010000419          362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.429, whole genome shotgun sequence.
ACCESSION   DASJON010000419 DASJON010000000
VERSION     DASJON010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.429"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>362
                     /locus_tag="U3989_003018"
     CDS             <1..>362
                     /locus_tag="U3989_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1716408.1"
                     /translation="DEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAVAINVNPDI
                     LIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSYWLKNGEVV
                     EYGDTMEVTTNYENFLKK"
BASE COUNT          116 a           52 c           79 g          115 t
ORIGIN      
        1 gatgaaatgg tcgatgatat cattgaattt gctgacatcg gagaccatat ttatcaacct
       61 gtaaaaactt attcaagcgg gatgttcgtt cgtctagctt ttgctgtagc aatcaatgtg
      121 aatcccgata ttttgattgt ggatgaagca ttggcagtag gcgatcttga atttcaacta
      181 aaatgtatgg aaaaatttac agaaatcaag aactcgggaa agacgatttt attcgtttct
      241 catgatatca gttcgattcg tcgtttttgt gatcgttcct attggttgaa aaacggagaa
      301 gtcgtcgaat atggcgatac gatggaagta acgacgaatt atgaaaactt tttaaagaaa
      361 aa
//
LOCUS       DASJON010000420          345 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.430, whole genome shotgun sequence.
ACCESSION   DASJON010000420 DASJON010000000
VERSION     DASJON010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.430"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          159 a           45 c           52 g           89 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaa
//
LOCUS       DASJON010000421          342 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.431, whole genome shotgun sequence.
ACCESSION   DASJON010000421 DASJON010000000
VERSION     DASJON010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.431"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..269
                     /locus_tag="U3989_003019"
                     /pseudo
     CDS             1..269
                     /locus_tag="U3989_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          124 a           46 c           64 g          108 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc ca
//
LOCUS       DASJON010000422          341 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.432, whole genome shotgun sequence.
ACCESSION   DASJON010000422 DASJON010000000
VERSION     DASJON010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.432"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>341
                     /locus_tag="U3989_003020"
     CDS             <1..>341
                     /locus_tag="U3989_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716409.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DASJON010000423          325 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.433, whole genome shotgun sequence.
ACCESSION   DASJON010000423 DASJON010000000
VERSION     DASJON010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.433"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..208)
                     /locus_tag="U3989_003021"
     CDS             complement(<1..208)
                     /locus_tag="U3989_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716410.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
BASE COUNT          129 a           61 c           29 g          106 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaa
//
LOCUS       DASJON010000424          321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.434, whole genome shotgun sequence.
ACCESSION   DASJON010000424 DASJON010000000
VERSION     DASJON010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.434"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           92 a           67 c           50 g          112 t
ORIGIN      
        1 ctgattgaac atcgtttatt ggagaggttg taaaaaagct gttctgcatc aagtaataag
       61 aacagccaaa taaaaacagc tcctaatgtt tttaacttgg ctggacttat taccgcagat
      121 gcaagctttt gaacaccgtt tattcgaaaa aaaaggactg tgacacggct tttgtcacag
      181 tccccttttt tcgaataatt attgtccccc tgtcaccttc acgatcacaa gtacattatt
      241 cctatgaatt aaattatttt tcttcatttt tcgttgaacc atcttcgttc aacattgctt
      301 ttcttgacag atttacgcgt c
//
LOCUS       DASJON010000425          318 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.435, whole genome shotgun sequence.
ACCESSION   DASJON010000425 DASJON010000000
VERSION     DASJON010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.435"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>318
                     /locus_tag="U3989_003022"
     CDS             <1..>318
                     /locus_tag="U3989_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1716411.1"
                     /translation="IIKHLNQAFNDFRVQEMKRLHQLGQRREAEKVKKNWRFLLKNRQ
                     KINHYDYKTWRSFRAPKYPLLTEAMMIDRLLAFSTPLKDAYTFFHELTEAFRDKESDL
                     FFT"
BASE COUNT          108 a           66 c           59 g           85 t
ORIGIN      
        1 atatcatcaa acatttgaat caggcgttca atgattttcg tgtacaagaa atgaaaagat
       61 tacaccagtt agggcaacga cgggaagccg agaaagtgaa aaagaactgg cgctttcttc
      121 taaaaaaccg tcaaaaaatc aatcactatg attacaaaac atggcgcagt tttcgtgcac
      181 ctaaataccc gctactcact gaagcaatga tgattgatcg attgctggca ttttcaacgc
      241 ctttaaagga cgcctatact tttttccatg agctgaccga agcctttaga gacaaggaat
      301 cagacttatt ttttacct
//
LOCUS       DASJON010000426          311 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.436, whole genome shotgun sequence.
ACCESSION   DASJON010000426 DASJON010000000
VERSION     DASJON010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.436"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U3989_003023"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U3989_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN1716412.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           98 a           46 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc a
//
LOCUS       DASJON010000427          305 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.437, whole genome shotgun sequence.
ACCESSION   DASJON010000427 DASJON010000000
VERSION     DASJON010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.437"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>305
                     /locus_tag="U3989_003024"
     CDS             <1..>305
                     /locus_tag="U3989_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1716413.1"
                     /translation="ITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEERE
                     KEMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQ"
BASE COUNT          126 a           46 c           70 g           63 t
ORIGIN      
        1 atcacagatt tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat
       61 tttgtttctg aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt
      121 gaagaacgtg aaaaagaaat ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag
      181 gcggaagaaa aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg
      241 caaggaaatg tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat
      301 caaga
//
LOCUS       DASJON010000428          302 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.438, whole genome shotgun sequence.
ACCESSION   DASJON010000428 DASJON010000000
VERSION     DASJON010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.438"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          107 a           33 c           48 g          114 t
ORIGIN      
        1 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacattc
       61 taatatattc atatgagcta attttatgat taaatggtaa gtttttcctt gtttgtttta
      121 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga tctgtggggt aaatcattat
      181 acaaaagtaa aattttactt attaacaggc tgtagaaata taaaaaagaa ctaaaattta
      241 actaaattat gtgctattct taaggaaagc caagtgtatg agaaagtttc tgagaatata
      301 at
//
LOCUS       DASJON010000429          299 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.439, whole genome shotgun sequence.
ACCESSION   DASJON010000429 DASJON010000000
VERSION     DASJON010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.439"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="U3989_003025"
     tRNA            complement(21..94)
                     /locus_tag="U3989_003025"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="U3989_003026"
     tRNA            complement(108..181)
                     /locus_tag="U3989_003026"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="U3989_003027"
     tRNA            complement(209..281)
                     /locus_tag="U3989_003027"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
BASE COUNT           82 a           83 c           73 g           61 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatt
//
LOCUS       DASJON010000430          291 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.440, whole genome shotgun sequence.
ACCESSION   DASJON010000430 DASJON010000000
VERSION     DASJON010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.440"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           98 a           51 c           49 g           93 t
ORIGIN      
        1 cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg tactgttcta
       61 tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt ttcaaaataa
      121 aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc tatacatcta
      181 attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa actttggaaa
      241 acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac t
//
LOCUS       DASJON010000431          284 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.441, whole genome shotgun sequence.
ACCESSION   DASJON010000431 DASJON010000000
VERSION     DASJON010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.441"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..>284
                     /locus_tag="U3989_003028"
     CDS             6..>284
                     /locus_tag="U3989_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEN1716414.1"
                     /translation="MTTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDS
                     YGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNT"
BASE COUNT          104 a           40 c           61 g           79 t
ORIGIN      
        1 aagctgtgac tacgagctta caagtggcag aaagcttcga gaaaaaacat caacatgttt
       61 taagagatat tgatgcacta aaaaaagatg tgtccaattt tggacagatg tttgtagaag
      121 gtaatgaacc agattcatat ggcagaaatc gacgagtttt cttcattagt agagatggat
      181 ttttcttgct ggctatgggg tttacaggaa agaaagctat ctacttcaaa caaaaataca
      241 ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa tact
//
LOCUS       DASJON010000432          275 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.442, whole genome shotgun sequence.
ACCESSION   DASJON010000432 DASJON010000000
VERSION     DASJON010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.442"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(58..142)
                     /locus_tag="U3989_003029"
     tRNA            complement(58..142)
                     /locus_tag="U3989_003029"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Leu,seq:caa)
     gene            complement(163..233)
                     /locus_tag="U3989_003030"
     tRNA            complement(163..233)
                     /locus_tag="U3989_003030"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Cys,seq:gca)
BASE COUNT           84 a           61 c           57 g           73 t
ORIGIN      
        1 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
       61 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      121 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      181 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      241 tattatcaat ctataaaata aagaataaat tatag
//
LOCUS       DASJON010000433          268 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.443, whole genome shotgun sequence.
ACCESSION   DASJON010000433 DASJON010000000
VERSION     DASJON010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.443"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..260
                     /locus_tag="U3989_003031"
     CDS             51..260
                     /locus_tag="U3989_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716415.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
BASE COUNT           97 a           26 c           48 g           97 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaa
//
LOCUS       DASJON010000434          264 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.444, whole genome shotgun sequence.
ACCESSION   DASJON010000434 DASJON010000000
VERSION     DASJON010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.444"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..>264)
                     /locus_tag="U3989_003032"
     CDS             complement(34..>264)
                     /locus_tag="U3989_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1716416.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           77 a           39 c           47 g          101 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaa
//
LOCUS       DASJON010000435          264 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.445, whole genome shotgun sequence.
ACCESSION   DASJON010000435 DASJON010000000
VERSION     DASJON010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.445"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           98 a           31 c           41 g           94 t
ORIGIN      
        1 taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc gatatttgag
       61 gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata tgaatgacat
      121 acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca ttcttttata
      181 tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat aaagaggttg
      241 tgaaaaagct gtcctgcatc aagt
//
LOCUS       DASJON010000436          262 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.446, whole genome shotgun sequence.
ACCESSION   DASJON010000436 DASJON010000000
VERSION     DASJON010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.446"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..241)
                     /locus_tag="U3989_003033"
     CDS             complement(<1..241)
                     /locus_tag="U3989_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1716417.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
BASE COUNT           67 a           50 c           48 g           97 t
ORIGIN      
        1 caatgtattc tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat
       61 tttctttgta ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga
      121 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
      181 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      241 tttatgttca ctctccttgt at
//
LOCUS       DASJON010000437          256 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.denovo.447, whole genome shotgun sequence.
ACCESSION   DASJON010000437 DASJON010000000
VERSION     DASJON010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.denovo.447"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          115 a           30 c           41 g           70 t
ORIGIN      
        1 attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa ataaatcaaa
       61 aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa atagtagatt
      121 tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag actaaactta
      181 aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat attttgaatg
      241 tgtttaaacg ttcaaa
//
LOCUS       DASJON010000438         9483 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.112, whole genome shotgun sequence.
ACCESSION   DASJON010000438 DASJON010000000
VERSION     DASJON010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.112"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..998)
                     /locus_tag="U3989_003034"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="U3989_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="U3989_003035"
     CDS             1489..2427
                     /locus_tag="U3989_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1716418.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="U3989_003036"
     CDS             2557..3204
                     /locus_tag="U3989_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HEN1716419.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3897..4835)
                     /locus_tag="U3989_003037"
                     /pseudo
     CDS             complement(3897..4835)
                     /locus_tag="U3989_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4835..5452)
                     /locus_tag="U3989_003038"
     CDS             complement(4835..5452)
                     /locus_tag="U3989_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1716420.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5466..5636)
                     /locus_tag="U3989_003039"
     CDS             complement(5466..5636)
                     /locus_tag="U3989_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716421.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5984..7474)
                     /locus_tag="U3989_003040"
     CDS             complement(5984..7474)
                     /locus_tag="U3989_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1716422.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(7517..7816)
                     /locus_tag="U3989_003041"
     CDS             complement(7517..7816)
                     /locus_tag="U3989_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716423.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8182..8466)
                     /locus_tag="U3989_003042"
     CDS             complement(8182..8466)
                     /locus_tag="U3989_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1716424.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(8985..9428)
                     /gene="vanZ"
                     /locus_tag="U3989_003043"
     CDS             complement(8985..9428)
                     /gene="vanZ"
                     /locus_tag="U3989_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN1716425.1"
                     /translation="MTLIWLVLFKLQYNILSVFNYHQRSLNLTPFTATGNFREMIDNV
                     IIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGATDITDVITNTVGGF
                     LGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINYV"
BASE COUNT         2954 a         1613 c         1484 g         3432 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt
     3541 taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt
     3601 ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc
     3661 tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt
     3721 taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa
     3781 taatttaaaa aataagcaaa aattttcgat agagaatgtg ctattttttg tcaaaggtgt
     3841 ataccttgac tgtgcttgct gttacattaa gtttattttt aagttattaa aaaagaaata
     3901 gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga ttgcaaacta
     3961 cttaaagaaa acaaactcgg actattcgtt ttcttctctt tggtttgaac atcagcaatt
     4021 atcccctctt gattgcctat tttagcttgt ttagaagaaa caaaagctaa aagctcctct
     4081 tgggttttaa aacgctgtgt ggggcttaga acgcccttaa acgacccttg gtttactttt
     4141 atactagctt ccacctcgaa aaaaggttct tttttaaaat tctctatggc ttcctggcgc
     4201 tgaaaaataa ggtataaggt gggcgtttga acacgtccta gtgaaaatgt accttgtacg
     4261 cccttctgtt gtaaatttaa cgtatacaaa gggcttgcgt tcatgccgat caaccaatcg
     4321 gcaatttggc gtgtttgcgc ttcttgataa aagggatagt aattcattcc aggttgcaaa
     4381 ttttgaaaac cgcttcggat tacatctttt tctaagctat tgatccatag tcttttaaat
     4441 gttttatctt ttgaaaaggc atttgcttta tggataatcg accaggcgat attttcacct
     4501 tctctgtcgc tatctgttgc aacaataatt gtatttgcct ttttgagaag ttctgcaaca
     4561 attttaaact gctttccctt atcttttgca acttcaaaat cgtatcgatc aggaaaaatc
     4621 ggcaaagatt caagtttcca attttgccac ttttcgtcat aatgacctgg ttctgctaat
     4681 tccactaaat gcccaaaacc aaaggtgata aacgtttcat ctgtaaatag tgggtctttg
     4741 atctcaaaat aaccgtcttt tttggtgctt tgttttaaag cacttgcgta ggctaatgcc
     4801 tggcttggtt tttcagctaa aataaccgta ctcattaact atccctcttt tcattgtttt
     4861 ttctttgatc gactgtcacg ttatatcttg ctcgatacct tctaaacgtt cggcgattga
     4921 ttccagtttg ttcttcaact tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat
     4981 gctttaaacg tggatcattt tctttaaatt tatgctgacg acctttgaat ttgccttttt
     5041 tcttagcaat ttcgattcct tgtgcctgac gttccttaat tttttttcgt tctgattctg
     5101 cttgatactt gtacaattca atgacaaggc tattaatcaa acgccttaaa ttttcatctt
     5161 caataccatt cattgagggt aaatttaaga cttccagggt tgccccctta atttgaattt
     5221 gattcatcaa ttctgttaat tctttattat ttcgtcctaa tcgatctaat tcagtaacaa
     5281 taacaatatc cccttcacga atatagttaa gcatagcttg taattgtggg cgttcgaccg
     5341 attgaccgct taatttgtct gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc
     5401 gatctaagtt ctgttctttg ctactgacac gtgcataacc aattttagcc attttcaacc
     5461 aacctctaaa attctctcgg ttgcaataac caatcagcaa tatctacttt ttcaatttca
     5521 aattgcttat cagaaattgt cttttcgtaa gcgataaaat cttgcgcata ttgttgctca
     5581 ttaaaaatag ccaccacttc gtcattttct aaaactcgat aaataaattt tttcatttta
     5641 ctcctcctat tatgcccaac ttaaatgacc tattcaccaa gtcaattata ctgctaaaat
     5701 catattagga caaataggta tactctaatg acctataaaa gatagaaaat taaaaaatca
     5761 agtgttcgct tcgctctcac tgcccctcga cgttttagta gcctttccct cacttcgttc
     5821 agtccaagcc aactaaaagt tttcgggcta ctctctcctt ctccccctaa taattaatta
     5881 aaatcttact ctgtatattt ctgctaatca ttcgctaaac agcaaagaaa aaacaaacac
     5941 gtatcataga tataaatgta atggcatagt gcgggtttta ttttcagcct gtatcatagc
     6001 taaacaaatc gagttgtgtg tccgttttag ggcgttctgc tagcttgttt aaagtctctt
     6061 gaatgaatgt atgctctaag tcaaaagaat ttgtcagcgc ctttatatag ctttcttttt
     6121 cttctttttt tactttaatg atcgatagca acaatgattt aacactagca agttgaatgc
     6181 caccatttct tcctggttta atcttaaaga aaatttcctg attcgccttc agtaccttca
     6241 gcaatttatc taatgtccgt tcaggaatgc ctagcacttc tctaatctct tttttggtcg
     6301 tcactaaata aggcttgtat acatcgcttt tttcgctaat ataagccatt aaatcttctt
     6361 tccattctga caaatgaaca cgttgacgtt cgcttctttt tttcttgaat ttaaaccacc
     6421 cttgacggac aaataaatct ttactggtta aatcacttga tacccaagct ttgcaaagaa
     6481 tggtaatgta ttccctatta gccccttgat agttttctga ataggcactt ctaacaattt
     6541 tgattacttc tttttcttct aagggttgat ctaatcgatt attaaactca aacatattat
     6601 attcgcacgt ttcgattgaa tagcctgaac taaagtaggc taaagagagg gtaaacatga
     6661 cgttattacg ccctattaaa cccttttctc ctgaaaattt cgtttcgtgc aataagagat
     6721 taaaccaggg ttcatctact tgttttttgc cttctgtacc gcttaaaacc gttagacttg
     6781 aacgagtaaa gcccttatta tctgtttgtt tgaaagacca atcttgccat tctttgaaag
     6841 aataacggta attaggatca aaaaattcta cattgtccgt tcttggtatg cgagcaatac
     6901 caaaatgatt acacgttaga tcaactggca aagactttcc aaaatattct cggatatttt
     6961 gcgaaattat tttggctgct ttgacagatt taaattctga ttttgaagtc acatagactg
     7021 gcgtttctaa aacaaaatat gcttgataac ctttatcaga tttgataatc atagtaggca
     7081 taaaacctaa atcaatagcg gttgttaaaa tatcgcttgc tgaaatagtt tcttttgccg
     7141 tgtgaatatc aaaatcaata aagaaggtat tgatttgtct taaattgttt tcagaatgtc
     7201 ctttcgtgta tgaacggttt tcgtctgcat acgttccata acgataaacg ttgggtgtcc
     7261 aatgtgtaaa tgtatcttga ttttcttgaa tcgcttcctc ggaagtcaga acaacaccac
     7321 gaccgccaat catgcttgat tttgagcgat acgcaaaaat agcccctttg cttttacctg
     7381 gcttggtagt gattgagcga attttactat ttttaaattt gtactttaac aagccgtcat
     7441 gaagcacagt ttctacaaca aaagggatat tcattcagct gttctccttt cctataaatc
     7501 ctataaaata ggttgtttaa ttaacttggt ttgctttttc attcaactgt ttcaatattg
     7561 catgttttga aaaagatttt tttcctttat aagtcaattt ttttccacta atcgaataaa
     7621 ttattttgtt attttctatt aacttatata tataatcttc cccctccgaa gaaaaatact
     7681 tatctgattt tgtttctaag tagatatttc tcttttctaa ctctttctta aacgtttcta
     7741 gtgtatagat atttgctaat tttcttatct ccaataaact attttttata taagttttac
     7801 attcatcatg attcatacaa actccacctt ctataaatga atacaaaaaa agcaatcaaa
     7861 cgatttccga ttgattgctt aacaattctt aaattcagta gcttagatac ttgaaaactc
     7921 tctgatttcc ctatataatg atagtacggt tatataccgt cttcaaacaa agttaattaa
     7981 ataacttctt acgagggaag agttcatctg actaactgat aagcgttggt ttggcaatct
     8041 tatcgggcta tgcatttata aaatgtcgtc aaacatttta taaatgtgtc atggctcttt
     8101 tttcgtttct attcagttcg ttgtttcgtt atatctagta taccgctttt aaaaaaaata
     8161 agcaacgatt tcgtgcatta ttcacacgaa gtcattgctt ttttcttctt ccatttctaa
     8221 atccaatgtt acttgttctg attctgtttc tggttctggt tctgttggct catttgggat
     8281 taaatccact actagcgttg agttagttaa ctttgcaatt tgttctagtg tttttatggt
     8341 tggatctgat tttcctgatt ctattcgtga ataatttgat ctactcattt ctaattcttg
     8401 ggagaatttt tcttttgatt tggtacctct catctttttt aagctttctc taaatgctag
     8461 ttccaagttt ctcccccctc tttttgtgta ataaaattca cgctttattt aaattatagc
     8521 ttttaatatc ttaataaaca agtgattaat cacttgtgat ttgtgattat tccaaaagta
     8581 ggtaatttca ccaacctttt tcaaaagttt ttatcgacga ttattggact ggatcgtgtt
     8641 tcctaaagcg attctaagcg catgtttaat tttaaagtgg gtaattctct ttaaaacgtc
     8701 ctacaaagat tcaaaaaaag catggctgct agtgggggta ccgtcaggaa aatgcggatt
     8761 tacaacgtta aggaaattta ttaaagagac caattcctca gcactaaaat tgataattgg
     8821 gctttctttg ttactatagt gttaggtaag ctttttactt tatcaccggt tttcccctca
     8881 cttcacacct acgggcgagt ttccccacat cccgttttcc atctaatcca attcattcaa
     8941 tatctttatg tgtatgaaag atcagattgc ttgatttaga catcttacac gtaatttatt
     9001 cttaaatggg tacggtaaac gagcaataat acgagcaaaa gtatacctac aaaaataata
     9061 actctgtcta attttttttg attcatatgc ttattgctta aaccatataa tttcagtcca
     9121 agaaagcctc caacagtatt tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag
     9181 ataaattgaa ttatttcaaa agtaagactt aaaaccagta caaaagcaaa cttaggtaaa
     9241 aatccgattt ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca
     9301 ttatctatca tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt
     9361 tgatgataat taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt
     9421 agtgtcacta aatataaagc tagcggttct gttgcaaagt tttaaataaa gaataaaatc
     9481 cct
//
LOCUS       DASJON010000439         8030 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.132, whole genome shotgun sequence.
ACCESSION   DASJON010000439 DASJON010000000
VERSION     DASJON010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.132"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..577)
                     /locus_tag="U3989_003044"
     CDS             complement(134..577)
                     /locus_tag="U3989_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1716426.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="U3989_003045"
     CDS             complement(795..2135)
                     /locus_tag="U3989_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1716427.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="U3989_003046"
     CDS             2404..3300
                     /locus_tag="U3989_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1716428.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="U3989_003047"
     CDS             complement(3450..4391)
                     /locus_tag="U3989_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1716429.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="U3989_003048"
     CDS             4517..5638
                     /locus_tag="U3989_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1716430.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="U3989_003049"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="U3989_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1716431.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="U3989_003050"
     CDS             7426..7992
                     /locus_tag="U3989_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1716432.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2667 a         1499 c         1590 g         2274 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc
//
LOCUS       DASJON010000440         6365 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.158, whole genome shotgun sequence.
ACCESSION   DASJON010000440 DASJON010000000
VERSION     DASJON010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.158"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            151..1419
                     /locus_tag="U3989_003051"
     CDS             151..1419
                     /locus_tag="U3989_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1716433.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1906..2370
                     /locus_tag="U3989_003052"
     CDS             1906..2370
                     /locus_tag="U3989_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1716434.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2354..2983
                     /locus_tag="U3989_003053"
     CDS             2354..2983
                     /locus_tag="U3989_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1716435.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            3337..4815
                     /gene="msr(C)"
                     /locus_tag="U3989_003054"
     CDS             3337..4815
                     /gene="msr(C)"
                     /locus_tag="U3989_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1716436.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4862..5308
                     /locus_tag="U3989_003055"
     CDS             4862..5308
                     /locus_tag="U3989_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1716437.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5507..6022)
                     /locus_tag="U3989_003056"
     CDS             complement(5507..6022)
                     /locus_tag="U3989_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1716438.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            6139..>6365
                     /locus_tag="U3989_003057"
     CDS             6139..>6365
                     /locus_tag="U3989_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1716439.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVML"
BASE COUNT         2100 a         1098 c         1279 g         1888 t
ORIGIN      
        1 agaatattct tcatttacgt ctttttatgc agaaaaagca aatttattaa aaagaaacaa
       61 atgttattta aatatggaca ttgacttgct tttacttttt tagttacact gttatttagg
      121 aaaagtttca taattcaaag gagaaaaaag atgaaagcaa agaatttatt atttagcgtt
      181 attcttttag gaaatctagc agttgccaac acaacagtgc tagctgaaga atcgacagaa
      241 actgtcgcaa ctcagacatc gagttcttca gaaacaggaa ttgaaaacga tacagaaagt
      301 actttgcagt cagctactgt ctcagaaaat acaatttcaa ccgaagaacc aacaactgaa
      361 acaacagaac aaacacaaac cgaagacagt acatcagcag aagctcagaa caatccagag
      421 aatgcagaag aaatcacgaa ccaattgaca gatgggcaaa cattgacaca tacccatcaa
      481 gcagatttgt ctgaattcga aaactatact ggctatgctt tttatgctcg ctcatctgct
      541 aattctcaac aagcgttcat tgatagtatt gcctcaactg cacaaagttt ggcaagtgcc
      601 aatgacttat atgcatcagt catgattgct caagccatcg ttgaaagtgg ctgggggaac
      661 agtactttgg cttctgcccc taattacaat ctatttggga ttaaaggaag ctataatggc
      721 caatctgtta cgatgcctac ttcggaatat gtgaatgggc aatggattac ggtgaatgcc
      781 gcttttagaa aatatccttc gtacaaagaa tcattgcaag ataacgtaac ggtattgaag
      841 acgacttctt ttcagccagg cgtttactac tattccggtg catggaaaag caatacgaac
      901 tcttacaaag atgctacggc ttggctaaca ggtcgttatg ccactgcgcc taattatggt
      961 tctacattga acaatgtaat cgaaacctat aatctgacac agtatgatac agcaccaact
     1021 tcaacttctc cgatgtatcg tctgtataac cgtcatacag gagaacattt atatacattg
     1081 aatgccggag aaaaaaatta tctgccaact gtaggctggg aatatgaagg gattgcttgg
     1141 caagcaccaa acagcggaca acctgtctac cgtttataca atccttacag tggagaccat
     1201 cattacacaa tggcacaatc tgagatcaac tctttaacaa aaatcggttg gcgttatgaa
     1261 ggtctaagtt tttattctgg tggttccaag ccaatttatc gactatttaa tccaaatgaa
     1321 aaaactggta cccatcacta taccttgagt gctaaggaaa gagattggct aacaccgatg
     1381 ggctggagat atgaaggaat aggattttat ggttattaaa aagaggtagt gaaaaagctg
     1441 ccctgcatca agtagtaaga acagccaaac aaaaacagat tttcatgttt tttgtttggt
     1501 tgaacttatt accgcagatg caagtttttg aacaccgttt actcaaaaaa ggttgtgaca
     1561 acatcttgtc ataacttttt tattactgtt taagcatact gatccaattt ctaatattcg
     1621 acgggattct tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt atctactgca
     1681 gaagagctat cttctatagt ttgataagaa tcaataatct tggttttagc gttgtgcttt
     1741 ctttatcatt ttttccgttc attctacaaa aaatttcttt tctctatact tttctattga
     1801 gagcgttttt tttattacac agctgattct ttgacttttt tcttttttta ttctattatt
     1861 gacaagtcaa tcgttgacga gtcaattatt agaaaggagg aaattatgtc tttagaagag
     1921 attagtcacc ttttatatca aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa
     1981 gaaaatatcg gtataagtct tactagatac gagatcatga tgagactgaa agaagaaaat
     2041 ccattgctac aaagcagttt gcaagaaaag ttgcagattg atcaaggagc agttactcgt
     2101 catctgaagg tattggaagc aaatggctat gtttcgcgtc agagaaatcc tcagaacaac
     2161 cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga aaatcaagca ttgtgaaatg
     2221 gaccataaag atacaggttc cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa
     2281 tttctaacac ttttagataa attgaatacc caactttgca atataacaac agaaacgaaa
     2341 gaggttaata ataatggaac aaaaaaatga tttttcaaca attatgactg gtagaagatc
     2401 cgttcgcgta tatgatgaaa catacaaaat cccacacgaa gaaatgctgg aaatgatcaa
     2461 agaagcgacg actgcacctt cttctgtcaa tatgaaacca tggcgctttg tggtcgctga
     2521 aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga
     2581 tacttcttct gcaatggtca tgatttttgg cgatatgcaa tgttacgaat atggcgaaga
     2641 aatctacaac caagcttacg aatcagggaa gatgccaaaa gaagtcagag atcaacaatt
     2701 agctgcgatc attccttatt acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa
     2761 agtcgattct agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc atggctatga
     2821 aacaaatcca attggtggat tcgaagcaga tcagttggca gaggcatttg gtttggataa
     2881 agaccgttat gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga
     2941 gtctgttcgt ttagcaccag aaaagatcac tatgttcgaa taaaaggttt tcagttacta
     3001 ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga
     3061 aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca
     3121 aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt
     3181 agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta
     3241 ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat
     3301 aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata
     3361 acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc
     3421 tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc
     3481 aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc
     3541 aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc
     3601 agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa
     3661 cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag
     3721 ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat
     3781 tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa
     3841 atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc
     3901 actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag
     3961 aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa
     4021 gtgtccagta aaaagaaaaa gcaacaaatc agactggatc ggttgtcttc ctctaaacaa
     4081 aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa
     4141 aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa
     4201 tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt
     4261 gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt
     4321 gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa
     4381 ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg
     4441 acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt
     4501 gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt
     4561 acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt
     4621 aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta
     4681 gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt
     4741 gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt
     4801 acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag
     4861 attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt
     4921 cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt
     4981 gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt
     5041 gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat
     5101 cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt
     5161 gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat
     5221 gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt
     5281 gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc
     5341 atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg
     5401 ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga
     5461 taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact
     5521 gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc
     5581 tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca
     5641 attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc
     5701 cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt
     5761 tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat
     5821 cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac
     5881 cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa
     5941 ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata
     6001 atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc
     6061 acagtaaagt ttccacgttt tacatgtgca tattgacatg taaaataaat catgtatgat
     6121 gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca
     6181 aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct
     6241 tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct
     6301 acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg
     6361 ctatt
//
LOCUS       DASJON010000441         4804 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.193, whole genome shotgun sequence.
ACCESSION   DASJON010000441 DASJON010000000
VERSION     DASJON010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.193"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..787
                     /gene="vanR"
                     /locus_tag="U3989_003058"
     CDS             92..787
                     /gene="vanR"
                     /locus_tag="U3989_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1716440.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="U3989_003059"
     CDS             765..1919
                     /locus_tag="U3989_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1716441.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="U3989_003060"
     CDS             2134..3102
                     /locus_tag="U3989_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1716442.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="U3989_003061"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="U3989_003061"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1716443.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="U3989_003062"
     CDS             4132..4740
                     /locus_tag="U3989_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN1716444.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1281 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccct
//
LOCUS       DASJON010000442         4286 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.210, whole genome shotgun sequence.
ACCESSION   DASJON010000442 DASJON010000000
VERSION     DASJON010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.210"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            110..607
                     /gene="dfrG"
                     /locus_tag="U3989_003063"
     CDS             110..607
                     /gene="dfrG"
                     /locus_tag="U3989_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1716445.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(679..2631)
                     /locus_tag="U3989_003064"
     CDS             complement(679..2631)
                     /locus_tag="U3989_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1716446.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2638..2874)
                     /locus_tag="U3989_003065"
     CDS             complement(2638..2874)
                     /locus_tag="U3989_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716447.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3276..3566
                     /locus_tag="U3989_003066"
     CDS             3276..3566
                     /locus_tag="U3989_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1716448.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1348 a          724 c          747 g         1467 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcat aatgagaaaa ggttttttga
       61 tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa tgaaagtttc
      121 tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca ttccttggag
      181 gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga taatattagg
      241 taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata ttattctgac
      301 gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag aagatgtttt
      361 tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga tttataattt
      421 gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat tcgaaggaga
      481 tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc aaaaagggat
      541 aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa aaaacttatt
      601 gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag agcttgggat
      661 tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa attgtttatc
      721 tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt taaaagggcg
      781 acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact tcggattgaa
      841 gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc tttcaagaga
      901 tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat taacatttaa
      961 tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac aatcaactga
     1021 ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg catatttgcg
     1081 atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg ttagtaattc
     1141 agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc tgatatctcc
     1201 ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt ttctgctttt
     1261 cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa
     1321 tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc gccattcgtc
     1381 ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag gagcatcaac
     1441 atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt gaagataagc
     1501 attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc aatctaacct
     1561 atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg tatcttcatt
     1621 taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca catatcttga
     1681 taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa accccgtata
     1741 tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca tcttgtccgt
     1801 attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat atcgttctaa
     1861 aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc caactccaat
     1921 tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct tagcgaacat
     1981 tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt ttggtagaac
     2041 attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc tacttttccc
     2101 ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg agcagccttc
     2161 tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta taaggcgttt
     2221 aatttcttca acaacagacc aattagataa aggggttact gtacagtttt gcgatacacc
     2281 tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt tggaactacc
     2341 agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct gttctttccc
     2401 aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt cagaaccgtt
     2461 ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa atcgttttgg
     2521 agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa tcggaatttt
     2581 aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca ttgtctatca
     2641 acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc gcttgttggc
     2701 aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat ccactgcttc
     2761 gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta cttgctctga
     2821 tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt gcataggaaa
     2881 atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt gcattcctac
     2941 aataattatt tcacgttcgc atttttatat acattttaac ttacattttg tggtgtaaat
     3001 taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg caatttgata
     3061 ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt aaatctatac
     3121 tgctgcctct tttttttagt acatgtcctt catcttcgca ggaagtgcca atatgatttt
     3181 aagaaaggta atagacaaaa agtcaaaaag ataaaacaga gtttcttctt tacaaaggga
     3241 atgtatgttc gtataatggt attgaggtga aggttatgga gaaaaaatat ggaacagtag
     3301 ataagatccg tactttgcaa gttagtgcca gaccgttgat ccgtttcagt ttaaatgggg
     3361 tcaattgttt gattgcttcc catagtttga acttcctagc tgaagtagac gaagggatga
     3421 agttagtcgt tacaggttat tataataata gaaagcaatt tgttgtgaga tcctatcatg
     3481 tgttagggaa acccaaaatt gtggtagaat atgagaaaag tctataccca agaaaaaagg
     3541 tccgacaaga tgaaagaata aactagaaaa caagcggctg agacaaaaat gtcctagccg
     3601 cttgttttgg aaagatggat gcagacgatg ggatttgaac ccacacgagc atacgctcac
     3661 aagaacctca atctatatat aactacatgt atttaagtat ttgtgatgaa tcactaaact
     3721 ttccaaactc atttgaggga tattgcttaa agattgaaca gatcaattcg aagccctatt
     3781 agtattcgct caatgggggg atcagaggcc ataaaaagag actagtcatt actagtctca
     3841 ggtagtggaa ttgtaggatt ttaaacgttc ctggagagta ccggtatgta actcgaagag
     3901 gtgattatca tagtcgtaaa agtaaattga agaagcctcg cctttcacac gagaacgtcc
     3961 tggttcgaca gttaaaccta acatttggat ttttgagaca aacgaatcta tatcttgttc
     4021 gtcaatttta aaagcaatat gattgtatgt ctttggcagt ttattcgacg aattctgcat
     4081 tactgcaatc cataggtcct taactaagaa aaatttttct ggatataatg aaaatttttt
     4141 tgttgactat cgtagatttc ttttgcatca aataaggtat ttaacaatta agtatttaaa
     4201 agataacttt taagttatct ttaagtaatg cttaaagcta tgttatactc tattaggaaa
     4261 tataagaagg agtataaaaa tgaaca
//
LOCUS       DASJON010000443         3277 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.241, whole genome shotgun sequence.
ACCESSION   DASJON010000443 DASJON010000000
VERSION     DASJON010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.241"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..188
                     /locus_tag="U3989_003067"
     CDS             <1..188
                     /locus_tag="U3989_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1716449.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="U3989_003068"
     CDS             203..1072
                     /locus_tag="U3989_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1716450.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="U3989_003069"
     CDS             1053..1787
                     /locus_tag="U3989_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1716451.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="U3989_003070"
     CDS             1820..2728
                     /locus_tag="U3989_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN1716452.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="U3989_003071"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="U3989_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          948 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccc
//
LOCUS       DASJON010000444         2878 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.258, whole genome shotgun sequence.
ACCESSION   DASJON010000444 DASJON010000000
VERSION     DASJON010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.258"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..220
                     /locus_tag="U3989_003072"
     CDS             <1..220
                     /locus_tag="U3989_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1716453.1"
                     /translation="YPIKEFIQHLKFQKLLDRYDDLYSMDMFGESIEGHKYKTPYQVV
                     KMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            374..451
                     /locus_tag="U3989_003073"
                     /pseudo
     CDS             374..451
                     /locus_tag="U3989_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            696..812
                     /locus_tag="U3989_003074"
     CDS             696..812
                     /locus_tag="U3989_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN1716454.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            828..2747
                     /gene="tet(M)"
                     /locus_tag="U3989_003075"
     CDS             828..2747
                     /gene="tet(M)"
                     /locus_tag="U3989_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN1716455.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          992 a          422 c          583 g          881 t
ORIGIN      
        1 ttacccaatc aaagaattta tccagcattt gaagtttcaa aaattattag accgctatga
       61 tgatttatac agtatggata tgtttgggga aagcatagag ggacataagt ataagacacc
      121 ttatcaagtc gtgaaaatga taaacccgaa ctcaaaagaa gatatggaac tgttacgaga
      181 agtattaagg aataacaatt ttgatgatga ggacgagtaa cttttttcct cttgcgagaa
      241 gatacagcaa acaaaaagcc cgatcaagag tgcaaagcac caccaatggt ggcaaagctc
      301 ttgacagggc tttttctttt ctgttgtggg caatcaagag gaagaaaggg ataaaatctg
      361 atttgtggta aatcaggcaa aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa
      421 gaacgggagt aattggaaga ttatagaata acaaatattg gtacattatt acagctattt
      481 tgtaatcacg tactctcttt gataaaaaat tggagattcc tttacaaata tgctcttacg
      541 tgctattatt taagtatcta tttaaaagga gttaataaat atgcggcaag gtattcttaa
      601 ataaactgtc aatttgatag tgggaacaaa taattggatg tcctttttta ggagggctta
      661 gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat ccggagaata
      721 tctgtatgct ttgtatgcct atggttatgc ataaaaatcc cagtgataaa agtatttatc
      781 actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta
      841 atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat
      901 ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata
      961 atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt
     1021 gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag
     1081 tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg
     1141 tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct
     1201 tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta
     1261 aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt
     1321 gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc
     1381 ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa
     1441 gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata
     1501 tagggattga taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag
     1561 gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc
     1621 ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag
     1681 aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga
     1741 ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata
     1801 gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc
     1861 tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc
     1921 ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg
     1981 aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag
     2041 aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt
     2101 taaaaaatgc agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca
     2161 ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt
     2221 ctcttggata cttaaatcaa tcgtttcaaa atgcagttat ggaggggata cgctatggct
     2281 gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt aagtatggct
     2341 tatactatag ccctgttagt accccagcag attttcggat gcttgctcct attgtattgg
     2401 aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt tttaaaattt
     2461 atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca
     2521 tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc cctgctcggt
     2581 gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt gtttgtttaa
     2641 cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa
     2701 atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt attttatgtt
     2761 gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa agatttgaat
     2821 taaagtgtaa aggaggagat agttattata aactacaagt ggatattgtg tcctgtat
//
LOCUS       DASJON010000445         1394 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.333, whole genome shotgun sequence.
ACCESSION   DASJON010000445 DASJON010000000
VERSION     DASJON010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.333"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            259..1170
                     /locus_tag="U3989_003076"
     CDS             259..1170
                     /locus_tag="U3989_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN1716456.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          504 a          199 c          278 g          413 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat
     1201 gaacagcatt attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac
     1261 gttaactagg acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta
     1321 tattgggaaa aatattatct agaggattgc tagcttggtt ctgttgcaaa gttttaaatc
     1381 tactatcaaa taag
//
LOCUS       DASJON010000446         1148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.351, whole genome shotgun sequence.
ACCESSION   DASJON010000446 DASJON010000000
VERSION     DASJON010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.351"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..76
                     /locus_tag="U3989_003077"
     CDS             11..76
                     /locus_tag="U3989_003077"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN1716457.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="U3989_003078"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="U3989_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1716458.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DASJON010000447         1098 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1090
            SAMN38756131-rid20573833.guided.353, whole genome shotgun sequence.
ACCESSION   DASJON010000447 DASJON010000000
VERSION     DASJON010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756131
            Sequence Read Archive: SRR27168115
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028268.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028268.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:08:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,045
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 15 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756131-rid20573833.guided.353"
                     /isolate="VE1090"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-07-11"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            382..930
                     /gene="aac(6')"
                     /locus_tag="U3989_003079"
     CDS             382..930
                     /gene="aac(6')"
                     /locus_tag="U3989_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1716459.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          385 a          160 c          217 g          336 t
ORIGIN      
        1 acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg
       61 cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta
      121 tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa
      181 acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt
      241 tctgtataat ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt
      301 ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag
      361 caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg
      421 aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg
      481 gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa
      541 gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg
      601 catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat
      661 tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat
      721 ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg
      781 gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa
      841 atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa
      901 acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc
      961 ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga aattccaact
     1021 tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta
     1081 gtttcgagta gtaagaaa
//
